Rw1G029540

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005782
Physical Location & Seq
Forward (+)
57253219 .. 57261053
7835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029540.1

Sequence Viewer

Length: 4134 bp
ATGGACATGAGCGGGGACACGTCGGCGCAGCCGGTCGAGCAGGAGGAGGAGATGGAGGAGCCCGAGGAGATCGAGCCGCCGCCCGCCGCCGTGCCGTTTTCGAGGCTGTTCACCTGCGCCGACCGCCTCGATTGGGTTTTGATGACCGTCGGATCGCTGGCGGCCGCCGCGCACGGCACGGCGCTCGTGGTGTACTTGCACTTTTTTGCTAAGATTATTCATCTGCTTTGGATCGATAAGCGTTCTGGAGAACCGCCGCCGGAGCAGTACCATATGCCGCCGGAGCAGTACCAGAAGTTTATGGATCTTGCTTTAAGCATTGTTTATATCGCTGTGGGTGTTTTTGCTGCTGGTTGGATTGAGGTCTCATGCTGGATTCTGACTGGAGAACGGCAGACTGCTGTCATCAGGTCAAGATATGTTCAAGTGTTACTTAACCAGGATATGAGTTTTTTTGATACCTATGGGAACAATGGGGACATCGTGAGTCAAGTTTTGAGTGATGTTCTACTCATTCAGTCTGCTCTCAGTGAAAAAGTTGGAAATTATATTCATAACATGGCGACATTTTTCAGTGGGCTCATTATTGGATTCATCAACTGTTGGCAGATTGCGGCTATAACATTAGCCACTGGTCCATTTATTGTTGCTGCTGGAGGAATATCAAATATATTTCTTCACAGGCTTGCTGAGAATATTCAAGATGCATATGCTGAAGCAGCTAGCATTGCTGAACAGGCAGTCTCTTACATTGGGACATTATATGCATTTACAAATGAAACATTGGCCAAGTATTCTTATGCAACATCACTACAAGCAACTCTTCGATATGGTATATTGATTAGTCTGGTGCAAGGACTAGGACTTGGATTTACATATGGGCTTGCCATTTGTTCATGTGCCTTGCAACTCTGGGTTGGAAGGTTCCTGGTTACTGATCGAAAAGCTCATGGTGGTGAAATTATAACAGCTTTATTTGCTGTAATATTAAGTGGCCTTGGGCTGAACCAAGCAGCAACAAACTTCTACTCATTTGATCAAGGTCGAATTGCGGCTTATAGACTGTTTGAGATGATACAAAGATCATCCTCTAATGTAAATCATGAGGGAACTATCCTAGTGGCTGTACAAGGAAATATTGAGTTCCGGAATGTGTATTTCAGTTATCCATCTCGTCCGGAGATTCCTATTTTAAGTAGTTTTTACCTCTCTGTACCTGCTAAGAAAGCTGTGGCACTCGTGGGTAGAAATGGATCTGGAAAGAGCAGTATCATTCCCCTCATGGAGCAGTTTTATAATCCAACATTAGGAGAAGTTCTTTTGGATGGAGAAAATATAAAAAACTTGAATTTGGAATGGCTCAGAAGCCAGATAGGGCTAGTCACACAAGATCCCGCCTTGTTAAGTTTGAGTATAAGAGATAATATTGCTTATGGGCGGGATGCGACAATGGATCAAATTGAAGAAGCTGCTAAAATAGCACATGCGCATACATTTATTACTTCGCTTGAGAGATCGTATGAAACACAGGTAGGAAGGGCAGGATTAGCACTGACAGAAGAGCAAAAAATAAAACTTTCAATTGCCAGGGCAGTACTTCTGAATCCATCAATTCTTCTGCTTGATGAGGTCACTGGTGGACTTGATTTTGAAGCTGAAAGAACTGTTCAAGAGGCTCTAGATCTCCTCATGTTGGGACGTTCAACTATAATTATAGCTCGGCGGCTTAGTCTTATAAGGAATGCTGATTACATAGCTGTGATGGAGGAAGGTCAACTAGTTGAAACGGGTACACATGAAGAGTTAATAACCCATGATGGCCTATATGCAGAGCTTCTCAGGTGTGAAGAAGCTGCAAAGCTTCCCAGGAGAATGCCAGCGCGGAATTACAAGGAGACTACAGCTTTCCAAATTGAAATGGATTCTTCAGCAAGTAATAGCTTCCAAGAACCATCATCCCCAAAAATGATGAAATCACCCTCACTTCAGAGGGTTACTGGTATATTTCGAATGGGTGACGTCAATTTTAATGCACAGGACTCACCAAAACCTAAGAGTCCACCAGTAGAGAAAGTGGTGGAAAACGGTCAGCAGCCCTTGGATACAGCAGATAAGGAACCATCAATAAAGCGGCAGGATAGTTTTGAAATGAGACTACCAGAGCTACCCAAGATTGACGTCCAGTCTGCAAATCAACAAACAACAAATGGTTCGGACCCTGAATCCCCTGTTTCACCCCTTTTGACATCTGATCCGAAAAATGAACGTTCCCATTCACAAACCTTTAGCCGCCCTCATAGCCATTCTGATGATGTTCCCATCAAAGTGAAGGGAGCAAAGAGTACACATTATAAAAAGTCACCATCATTTTGGAGGCTTGCACAGCTTTCTTTTGCAGAGTGGCTTTATGCTGTGTTAGGAAGCATTGGTGCTGCTATCTTTGGTTCCTTTAATCCTCTTCTTGCTTATGTTATCGCACTTGTAGTAACAGCATACTACAGGGGTGATGAAGGTCGTCACTTGAGGCAAGAAGTAGACAAATGGTGCTTGATCATTGCTTGCATGGGTATAGTGACAGTCGTTGCCAATTTCTTGCAACACTTCTACTTTGGTATCATGGGGGAGAAAATGACTGAACGAGTTCGCAGAATGATGTTTTCAGCAATGCTCCGTAATGAGGTGGGATGGTTTGATGAAGAGGAAAACAGTGCTGATACTTTGTCCATGCGCTTGGCAAATGATGCTACATTTGTGCGAGCTGCTTTTAGTAATCGGCTTTCAATATTCATACAGGATAGTGCTGCTATCATTGTGGCTGTTTTGATTGGGATGTTGCTACAATGGCGATTAGCACTTGTGGCTTTGGCAACCCTACCTGTTCTCACTATTTCTGCCATTGCACAGAAATTATGGCTTGCTGGTTTTTCAAGGGGGATTCAGGAGATGCACAGGAAAGCATCTTTGGTTCTCGAGGATGCAGTTAGAAACATTTACACTGTGGTAGCATTCTGTGCTGGTAACAAGGTAATGGAGCTCTACAGATTGCAGCTAAAGAAGATCTTTAAGCAGAGTTTCTTTCATGGAATGGCCATTGGGTTTGCATTTGGTTTTTCACAGTTCCTTCTTTTTGCCTGCAATGCTCTTCTCCTCTGGTACACTGCATACTCCGTGAAACAGGAGTATATGAATCTACCTACTGCTCTCAAGGAGTATATGGTTTTCTCTTTTGCAACATTCGCACTGGTAGAGCCCTTTGGGTTGGCTCCTTACATACTCAAACGACGTAAATCTCTCATTTCGGTATTTGAAATCATAGATCGAGTGCCAAAGATTGAGCCAGATGAAAGCTCCGCCATGAAACCACCTAATGTTTATGGAAGCCTTGAGTTGAAAAATGTTGATTTCTGTTATCCAACCCGCCCGGAACTGTTGGTGCTGAGCAATTTCAGTCTCAAAGTAAACGGGGGTCAAACTGTAGCTGTGGTGGGAGTTTCAGGGTCGGGTAAGAGCACTATAATTTCTTTGATCGAGAGATTTTATGATCCAGTTGCTGGTCAAGTGATGCTGGATGGTCGAGATCTGAAGCTTTATAATTTGAGATGGTTGAGGAACCACCTTGGTCTTGTTCAACAGGAGCCCATTATTTTCTCGACAACAATAAGGGAGAATATAATATATGCAAGGCATAATGCTAGTGAAGCTGAAATGAAAGAGGCTGCAAGAATAGCGAATGCTCATCATTTCATCAGCAGCTTGCCTCATGGTTATGATACACATGTGGGGATGAGAGGGGTTGATCTAACCCCAGGACAGAAGCAAAGAATTGCAATCGCTCGTGTTGTTTTAAAAAATGCTCCCATTTTGTTATTGGACGAAGCAAGTTCGTCTATTGAATCTGAGTCAAGTCGAGTGGTGCAGGAGGCCCTTGATACATTAATCATGGGGAACAAAACAACCATCTTGATAGCTCACAGAGCTGCGATGATGAGGCATGTTGACAACATTGTAGTTCTTAATGGAGGGAGAATTGTGGAAGAAGGGTCCCATGATTCTTTAATGTCAAAGAACGGTCTGTATGTCCGTTTGATGCATCCTCACTTTGGCAAGGGCCTGCGTCAGCACCGCCTTGTTTAG

Protein Analysis

1377

Amino Acids

153.16

Weight (kDa)

6.19

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 53 - 330 7.7e-40 ABC transporter transmembrane region
ABC_tran PF00005 401 - 545 4.7e-22 ABC transporter
ABC_membrane PF00664 809 - 1071 1.1e-39 ABC transporter transmembrane region
ABC_tran PF00005 1146 - 1294 1.9e-28 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 965, 1296, 1736, 2352, 3591
AarI CACCTGC 1 cut(s) 122
AatII GACGTC 2 cut(s) 2022, 2181
Acc16I TGCGCA 1 cut(s) 1488
Acc36I ACCTGC 2 cut(s) 122, 1225
AccB7I CCANNNNNTGG 1 cut(s) 3551
AccBSI CCGCTC 1 cut(s) 12
AccI GTMKAC 1 cut(s) 2535
AccII CGCG 2 cut(s) 170, 1882
AccIII TCCGGA 2 cut(s) 1146, 1177
AclI AACGTT 1 cut(s) 2266
AclWI GGATC 8 cut(s) 160, 239, 312, 1261, 1385, 1461, 2246, 3536
AcoI YGGCCR 3 cut(s) 162, 786, 3087
AcsI RAATTY 1 cut(s) 1348
AcuI CTGAAG 4 cut(s) 735, 1911, 1970, 3602
AcyI GRCGYC 2 cut(s) 2019, 2178
AfaI GTAC 9 cut(s) 194, 269, 290, 1128, 1215, 1596, 1792, 2344, 3155
AfiI CCNNNNNNNGG 6 cut(s) 133, 1307, 1693, 2677, 3551, 4100
AflIII ACRYGT 2 cut(s) 18, 3775
AhdI GACNNNNNGTC 1 cut(s) 2182
AhlI ACTAGT 1 cut(s) 1777
AjiI CACGTC 1 cut(s) 21
AjnI CCWGG 5 cut(s) 438, 927, 1586, 1865, 3805
AjuI GAANNNNNNNTTGG 2 cut(s) 2945, 2977
Alw21I GWGCWC 2 cut(s) 3036, 3512
Alw26I GTCTC 5 cut(s) 370, 748, 1889, 2146, 3455
AlwI GGATC 8 cut(s) 160, 239, 312, 1261, 1385, 1461, 2246, 3536
AlwNI CAGNNNCTG 1 cut(s) 3551
Ama87I CYCGRG 2 cut(s) 62, 2969
Aor13HI TCCGGA 2 cut(s) 1146, 1177
AoxI GGCC 7 cut(s) 162, 786, 994, 1819, 3087, 3921, 4108
ApoI RAATTY 1 cut(s) 1348
AseI ATTAAT 1 cut(s) 3935
Asp700I GAANNNNTTC 1 cut(s) 2640
AspLEI GCGC 7 cut(s) 28, 119, 172, 184, 1489, 1882, 2730
AspS9I GGNCC 5 cut(s) 635, 2215, 3922, 4041, 4108
AsuC2I CCSGG 1 cut(s) 3422
AsuHPI GGTGA 8 cut(s) 103, 968, 1968, 2027, 2034, 2226, 2352, 2516
AsuII TTCGAA 1 cut(s) 2008
AsuNHI GCTAGC 1 cut(s) 722
AvaI CYCGRG 2 cut(s) 62, 2969
AvaII GGWCC 3 cut(s) 635, 2215, 4041
BaeI ACNNNNGTAYC 4 cut(s) 2596, 2629, 3762, 3795
BalI TGGCCA 2 cut(s) 788, 3089
BanII GRGCYC 5 cut(s) 63, 582, 3036, 3252, 3639
BauI CACGAG 3 cut(s) 185, 1238, 3834
Bbv12I GWGCWC 2 cut(s) 3036, 3512
BceAI ACGGC 5 cut(s) 74, 79, 190, 195, 407
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 5 cut(s) 440, 929, 1588, 1867, 3807
BciVI GTATCC 1 cut(s) 2095
BclI TGATCA 2 cut(s) 1036, 2550
BcnI CCSGG 1 cut(s) 3422
BcoDI GTCTC 5 cut(s) 370, 748, 1889, 2146, 3455
BcuI ACTAGT 1 cut(s) 1777
BfaI CTAG 7 cut(s) 723, 860, 1118, 1379, 1679, 1778, 3693
BfmI CTRYAG 4 cut(s) 1899, 2497, 3037, 3474
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 2 cut(s) 122, 1225
BfuI GTATCC 1 cut(s) 2095
BglII AGATCT 3 cut(s) 1681, 3057, 3577
BlpI GCTNAGC 1 cut(s) 3437
BmcAI AGTACT 1 cut(s) 1596
Bme1390I CCNGG 6 cut(s) 440, 929, 1588, 1867, 3422, 3807
Bme18I GGWCC 3 cut(s) 635, 2215, 4041
BmeRI GACNNNNNGTC 1 cut(s) 2182
BmeT110I CYCGRG 2 cut(s) 62, 2969
BmgBI CACGTC 1 cut(s) 21
BmgT120I GGNCC 5 cut(s) 635, 2215, 3922, 4041, 4108
BmrFI CCNGG 6 cut(s) 440, 929, 1588, 1867, 3422, 3807
BmsI GCATC 9 cut(s) 694, 1432, 2731, 2934, 2965, 2966, 3552, 4077, 4099
BmtI GCTAGC 1 cut(s) 726
BoxI GACNNNNGTC 1 cut(s) 401
BpmI CTGGAG 3 cut(s) 267, 405, 675
Bpu1102I GCTNAGC 1 cut(s) 3437
Bpu14I TTCGAA 1 cut(s) 2008
BpuEI CTTGAG 4 cut(s) 1529, 2542, 3188, 3404
BpuMI CCSGG 1 cut(s) 3422
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 2 cut(s) 2019, 2178
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 7 cut(s) 63, 997, 1587, 1865, 2097, 3616, 3805
BsaWI WCCGGW 2 cut(s) 1146, 1177
Bsc4I CCNNNNNNNGG 6 cut(s) 133, 1307, 1693, 2677, 3551, 4100
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 8 cut(s) 388, 637, 1639, 2002, 2063, 2182, 3246, 3545
Bse3DI GCAATG 5 cut(s) 726, 2553, 2670, 2895, 3142
BseAI TCCGGA 2 cut(s) 1146, 1177
BseBI CCWGG 5 cut(s) 440, 929, 1588, 1867, 3807
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 7 cut(s) 63, 997, 1587, 1865, 2097, 3616, 3805
BseLI CCNNNNNNNGG 6 cut(s) 133, 1307, 1693, 2677, 3551, 4100
BseMI GCAATG 5 cut(s) 726, 2553, 2670, 2895, 3142
BseMII CTCAG 6 cut(s) 541, 681, 1375, 1852, 3428, 3888
BseNI ACTGG 8 cut(s) 388, 637, 1639, 2002, 2063, 2182, 3246, 3545
BseRI GAGGAG 6 cut(s) 59, 62, 71, 80, 1676, 3137
BseX3I CGGCCG 1 cut(s) 162
BsgI GTGCAG 1 cut(s) 3935
Bsh1236I CGCG 2 cut(s) 170, 1882
Bsh1285I CGRYCG 3 cut(s) 36, 124, 165
BshFI GGCC 7 cut(s) 164, 788, 996, 1821, 3089, 3923, 4110
BshVI ATCGAT 1 cut(s) 234
BsiEI CGRYCG 3 cut(s) 36, 124, 165
BsiHKAI GWGCWC 2 cut(s) 3036, 3512
BsiHKCI CYCGRG 2 cut(s) 62, 2969
BsiSI CCGG 6 cut(s) 32, 260, 281, 1147, 1178, 3422
BslFI GGGAC 5 cut(s) 29, 491, 769, 1710, 4027
BslI CCNNNNNNNGG 6 cut(s) 133, 1307, 1693, 2677, 3551, 4100
BsmAI GTCTC 5 cut(s) 370, 748, 1889, 2146, 3455
BsmFI GGGAC 5 cut(s) 29, 491, 769, 1710, 4027
BsmI GAATGC 4 cut(s) 1747, 1878, 3005, 3736
BsnI GGCC 7 cut(s) 164, 788, 996, 1821, 3089, 3923, 4110
Bso31I GGTCTC 1 cut(s) 370
BsoBI CYCGRG 2 cut(s) 62, 2969
Bsp119I TTCGAA 1 cut(s) 2008
Bsp1286I GDGCHC 6 cut(s) 63, 582, 3036, 3252, 3512, 3639
Bsp13I TCCGGA 2 cut(s) 1146, 1177
Bsp1407I TGTACA 1 cut(s) 1126
Bsp1720I GCTNAGC 1 cut(s) 3437
BspANI GGCC 7 cut(s) 164, 788, 996, 1821, 3089, 3923, 4110
BspCNI CTCAG 6 cut(s) 540, 682, 1374, 1851, 3429, 3889
BspDI ATCGAT 1 cut(s) 234
BspEI TCCGGA 2 cut(s) 1146, 1177
BspFNI CGCG 2 cut(s) 170, 1882
BspHI TCATGA 1 cut(s) 1102
BspMI ACCTGC 2 cut(s) 122, 1225
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 8 cut(s) 160, 239, 312, 1261, 1385, 1461, 2246, 3536
BspQI GCTCTTC 2 cut(s) 1554, 3147
BspT104I TTCGAA 1 cut(s) 2008
BspTNI GGTCTC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 12
BsrDI GCAATG 5 cut(s) 726, 2553, 2670, 2895, 3142
BsrFI RCCGGY 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 1126
BsrI ACTGG 8 cut(s) 388, 637, 1639, 2002, 2063, 2182, 3246, 3545
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 7 cut(s) 63, 997, 1587, 1865, 2097, 3616, 3805
BssNI GRCGYC 2 cut(s) 2019, 2178
BssSI CACGAG 3 cut(s) 185, 1238, 3834
BssT1I CCWWGG 3 cut(s) 997, 2097, 3616
Bst2BI CACGAG 3 cut(s) 185, 1238, 3834
Bst2UI CCWGG 5 cut(s) 440, 929, 1588, 1867, 3807
Bst6I CTCTTC 6 cut(s) 828, 1554, 1794, 2463, 2691, 3147
BstACI GRCGYC 2 cut(s) 2019, 2178
BstAPI GCANNNNNTGC 2 cut(s) 2741, 3011
BstAUI TGTACA 1 cut(s) 1126
BstBI TTCGAA 1 cut(s) 2008
BstFNI CGCG 2 cut(s) 170, 1882
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 7 cut(s) 28, 119, 172, 184, 1489, 1882, 2730
BstMAI GTCTC 5 cut(s) 370, 748, 1889, 2146, 3455
BstMCI CGRYCG 3 cut(s) 36, 124, 165
BstNI CCWGG 5 cut(s) 440, 929, 1588, 1867, 3807
BstNSI RCATGY 3 cut(s) 1487, 3779, 3995
BstPAI GACNNNNGTC 1 cut(s) 401
BstSCI CCNGG 6 cut(s) 438, 927, 1586, 1865, 3420, 3805
BstSFI CTRYAG 4 cut(s) 1899, 2497, 3037, 3474
BstUI CGCG 2 cut(s) 170, 1882
BstX2I RGATCY 6 cut(s) 304, 1253, 1390, 1681, 3057, 3577
BstXI CCANNNNNNTGG 2 cut(s) 2370, 2731
BstYI RGATCY 6 cut(s) 304, 1253, 1390, 1681, 3057, 3577
BstZI CGGCCG 1 cut(s) 162
Bsu15I ATCGAT 1 cut(s) 234
BsuI GTATCC 1 cut(s) 2095
BsuRI GGCC 7 cut(s) 164, 788, 996, 1821, 3089, 3923, 4110
BsuTUI ATCGAT 1 cut(s) 234
BtgZI GCGATG 1 cut(s) 3995
BtrI CACGTC 1 cut(s) 21
BtsI GCAGTG 1 cut(s) 3156
BtsIMutI CAGTG 9 cut(s) 535, 580, 630, 1550, 1632, 2713, 2994, 3156, 3239
BveI ACCTGC 2 cut(s) 122, 1225
CaiI CAGNNNCTG 1 cut(s) 3551
CciI TCATGA 1 cut(s) 1102
CciNI GCGGCCGC 1 cut(s) 162
CfoI GCGC 7 cut(s) 28, 119, 172, 184, 1489, 1882, 2730
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 5 cut(s) 635, 2215, 3922, 4041, 4108
ClaI ATCGAT 1 cut(s) 234
CseI GACGC 1 cut(s) 4103
Csp6I GTAC 9 cut(s) 193, 268, 289, 1127, 1214, 1595, 1791, 2343, 3154
CspCI CAANNNNNGTGG 2 cut(s) 3891, 3926
CviQI GTAC 9 cut(s) 193, 268, 289, 1127, 1214, 1595, 1791, 2343, 3154
DraI TTTAAA 1 cut(s) 3846
DriI GACNNNNNGTC 1 cut(s) 2182
EaeI YGGCCR 3 cut(s) 162, 786, 3087
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 6 cut(s) 828, 1554, 1794, 2463, 2691, 3147
Eam1105I GACNNNNNGTC 1 cut(s) 2182
EarI CTCTTC 6 cut(s) 828, 1554, 1794, 2463, 2691, 3147
EciI GGCGGA 1 cut(s) 3340
Ecl136II GAGCTC 1 cut(s) 3034
EclXI CGGCCG 1 cut(s) 162
Eco130I CCWWGG 3 cut(s) 997, 2097, 3616
Eco24I GRGCYC 5 cut(s) 63, 582, 3036, 3252, 3639
Eco31I GGTCTC 1 cut(s) 370
Eco47I GGWCC 3 cut(s) 635, 2215, 4041
Eco52I CGGCCG 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 3034
Eco57I CTGAAG 4 cut(s) 735, 1911, 1970, 3602
Eco88I CYCGRG 2 cut(s) 62, 2969
EcoICRI GAGCTC 1 cut(s) 3034
EcoO109I RGGNCCY 3 cut(s) 3922, 4041, 4108
EcoRII CCWGG 5 cut(s) 438, 927, 1586, 1865, 3805
EcoT14I CCWWGG 3 cut(s) 997, 2097, 3616
EcoT22I ATGCAT 3 cut(s) 709, 769, 4092
EcoT38I GRGCYC 5 cut(s) 63, 582, 3036, 3252, 3639
ErhI CCWWGG 3 cut(s) 997, 2097, 3616
FalI AAGNNNNNCTT 6 cut(s) 417, 449, 807, 839, 3044, 3076
FaqI GGGAC 5 cut(s) 29, 491, 769, 1710, 4027
FauI CCCGC 5 cut(s) 5, 91, 1402, 1431, 3425
FauNDI CATATG 3 cut(s) 273, 709, 877
FbaI TGATCA 2 cut(s) 1036, 2550
FblI GTMKAC 1 cut(s) 2535
FriOI GRGCYC 5 cut(s) 63, 582, 3036, 3252, 3639
FspAI RTGCGCAY 1 cut(s) 1488
FspBI CTAG 7 cut(s) 723, 860, 1118, 1379, 1679, 1778, 3693
FspI TGCGCA 1 cut(s) 1488
GlaI GCGC 7 cut(s) 27, 118, 171, 183, 1488, 1881, 2729
GsuI CTGGAG 3 cut(s) 267, 405, 675
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 7 cut(s) 164, 788, 996, 1821, 3089, 3923, 4110
HapII CCGG 6 cut(s) 32, 260, 281, 1147, 1178, 3422
HgaI GACGC 1 cut(s) 4103
HhaI GCGC 7 cut(s) 28, 119, 172, 184, 1489, 1882, 2730
Hin1I GRCGYC 2 cut(s) 2019, 2178
Hin6I GCGC 7 cut(s) 26, 117, 170, 182, 1487, 1880, 2728
HinP1I GCGC 7 cut(s) 26, 117, 170, 182, 1487, 1880, 2728
HincII GTYRAC 2 cut(s) 1775, 3997
HindII GTYRAC 2 cut(s) 1775, 3997
HindIII AAGCTT 2 cut(s) 1859, 3584
HpaII CCGG 6 cut(s) 32, 260, 281, 1147, 1178, 3422
HphI GGTGA 8 cut(s) 103, 968, 1968, 2027, 2034, 2226, 2352, 2516
Hpy99I CGWCG 3 cut(s) 25, 152, 3285
HpyAV CCTTC 7 cut(s) 915, 1530, 1763, 2323, 2504, 3131, 4031
HpyCH4IV ACGT 6 cut(s) 20, 1699, 2019, 2178, 2266, 3283
HpySE526I ACGT 6 cut(s) 20, 1699, 2019, 2178, 2266, 3283
Hsp92I GRCGYC 2 cut(s) 2019, 2178
HspAI GCGC 7 cut(s) 26, 117, 170, 182, 1487, 1880, 2728
KflI GGGWCCC 1 cut(s) 4041
Kpn2I TCCGGA 2 cut(s) 1146, 1177
Ksp22I TGATCA 2 cut(s) 1036, 2550
LguI GCTCTTC 2 cut(s) 1554, 3147
LweI GCATC 9 cut(s) 694, 1432, 2731, 2934, 2965, 2966, 3552, 4077, 4099
MaeI CTAG 7 cut(s) 723, 860, 1118, 1379, 1679, 1778, 3693
MaeII ACGT 6 cut(s) 20, 1699, 2019, 2178, 2266, 3283
MbiI CCGCTC 1 cut(s) 12
MfeI CAATTG 1 cut(s) 1581
MflI RGATCY 6 cut(s) 304, 1253, 1390, 1681, 3057, 3577
MhlI GDGCHC 6 cut(s) 63, 582, 3036, 3252, 3512, 3639
MlsI TGGCCA 2 cut(s) 788, 3089
MluNI TGGCCA 2 cut(s) 788, 3089
MlyI GAGTC 4 cut(s) 496, 2033, 2065, 3908
MmeI TCCRAC 6 cut(s) 130, 335, 520, 898, 1325, 3437
Mox20I TGGCCA 2 cut(s) 788, 3089
Mph1103I ATGCAT 3 cut(s) 709, 769, 4092
MroI TCCGGA 2 cut(s) 1146, 1177
MroXI GAANNNNTTC 1 cut(s) 2640
MscI TGGCCA 2 cut(s) 788, 3089
MslI CAYNNNNRTG 5 cut(s) 954, 1757, 2307, 2324, 3765
Msp20I TGGCCA 2 cut(s) 788, 3089
MspI CCGG 6 cut(s) 32, 260, 281, 1147, 1178, 3422
MspR9I CCNGG 6 cut(s) 440, 929, 1588, 1867, 3422, 3807
MunI CAATTG 1 cut(s) 1581
Mva1269I GAATGC 4 cut(s) 1747, 1878, 3005, 3736
MvaI CCWGG 5 cut(s) 440, 929, 1588, 1867, 3807
MvnI CGCG 2 cut(s) 170, 1882
NciI CCSGG 1 cut(s) 3422
NdeI CATATG 3 cut(s) 273, 709, 877
NheI GCTAGC 1 cut(s) 722
NmeAIII GCCGAG 1 cut(s) 1699
NmuCI GTSAC 6 cut(s) 1381, 1630, 2015, 2358, 2516, 2572
NotI GCGGCCGC 1 cut(s) 162
NsbI TGCGCA 1 cut(s) 1488
NsiI ATGCAT 3 cut(s) 709, 769, 4092
NspI RCATGY 3 cut(s) 1487, 3779, 3995
NspV TTCGAA 1 cut(s) 2008
PaeR7I CTCGAG 1 cut(s) 2969
PagI TCATGA 1 cut(s) 1102
PaqCI CACCTGC 1 cut(s) 122
PciI ACATGT 1 cut(s) 3775
PciSI GCTCTTC 2 cut(s) 1554, 3147
PctI GAATGC 4 cut(s) 1747, 1878, 3005, 3736
PdmI GAANNNNTTC 1 cut(s) 2640
PflMI CCANNNNNTGG 1 cut(s) 3551
PleI GAGTC 4 cut(s) 495, 2033, 2064, 3907
PpsI GAGTC 4 cut(s) 495, 2033, 2064, 3907
PpuMI RGGWCCY 1 cut(s) 4041
PscI ACATGT 1 cut(s) 3775
PshAI GACNNNNGTC 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 3935
PsiI TTATAA 5 cut(s) 965, 1296, 1736, 2352, 3591
Psp124BI GAGCTC 1 cut(s) 3036
Psp1406I AACGTT 1 cut(s) 2266
Psp5II RGGWCCY 1 cut(s) 4041
Psp6I CCWGG 5 cut(s) 438, 927, 1586, 1865, 3805
PspGI CCWGG 5 cut(s) 438, 927, 1586, 1865, 3805
PspPI GGNCC 5 cut(s) 635, 2215, 3922, 4041, 4108
PspPPI RGGWCCY 1 cut(s) 4041
PstNI CAGNNNCTG 1 cut(s) 3551
PsuI RGATCY 6 cut(s) 304, 1253, 1390, 1681, 3057, 3577
RsaI GTAC 9 cut(s) 194, 269, 290, 1128, 1215, 1596, 1792, 2344, 3155
RsaNI GTAC 9 cut(s) 193, 268, 289, 1127, 1214, 1595, 1791, 2343, 3154
RseI CAYNNNNRTG 5 cut(s) 954, 1757, 2307, 2324, 3765
SacI GAGCTC 1 cut(s) 3036
SapI GCTCTTC 2 cut(s) 1554, 3147
Sau96I GGNCC 5 cut(s) 635, 2215, 3922, 4041, 4108
ScaI AGTACT 1 cut(s) 1596
SchI GAGTC 4 cut(s) 496, 2033, 2065, 3908
ScrFI CCNGG 6 cut(s) 440, 929, 1588, 1867, 3422, 3807
SduI GDGCHC 6 cut(s) 63, 582, 3036, 3252, 3512, 3639
SfaNI GCATC 9 cut(s) 694, 1432, 2731, 2934, 2965, 2966, 3552, 4077, 4099
SfcI CTRYAG 4 cut(s) 1899, 2497, 3037, 3474
Sfr274I CTCGAG 1 cut(s) 2969
SfuI TTCGAA 1 cut(s) 2008
SinI GGWCC 3 cut(s) 635, 2215, 4041
SlaI CTCGAG 1 cut(s) 2969
SmiMI CAYNNNNRTG 5 cut(s) 954, 1757, 2307, 2324, 3765
SmlI CTYRAG 5 cut(s) 1508, 2521, 2969, 3203, 3383
SmoI CTYRAG 5 cut(s) 1508, 2521, 2969, 3203, 3383
SpeI ACTAGT 1 cut(s) 1777
SspI AATATT 5 cut(s) 697, 987, 1138, 1426, 2784
SspMI CTAG 7 cut(s) 723, 860, 1118, 1379, 1679, 1778, 3693
SstI GAGCTC 1 cut(s) 3036
StyD4I CCNGG 6 cut(s) 438, 927, 1586, 1865, 3420, 3805
StyI CCWWGG 3 cut(s) 997, 2097, 3616
TaiI ACGT 6 cut(s) 23, 1702, 2022, 2181, 2269, 3286
TatI WGTACW 4 cut(s) 192, 1126, 1594, 2342
TscAI CASTG 9 cut(s) 535, 580, 637, 1557, 1639, 2713, 3001, 3163, 3246
TseFI GTSAC 6 cut(s) 1381, 1630, 2015, 2358, 2516, 2572
Tsp45I GTSAC 6 cut(s) 1381, 1630, 2015, 2358, 2516, 2572
TspGWI ACGGA 3 cut(s) 2660, 3157, 4070
TspRI CASTG 9 cut(s) 535, 580, 637, 1557, 1639, 2713, 3001, 3163, 3246
Van91I CCANNNNNTGG 1 cut(s) 3551
VpaK11BI GGWCC 3 cut(s) 635, 2215, 4041
VspI ATTAAT 1 cut(s) 3935
XapI RAATTY 1 cut(s) 1348
XbaI TCTAGA 1 cut(s) 1678
XceI RCATGY 3 cut(s) 1487, 3779, 3995
XcmI CCANNNNNNNNNTGG 1 cut(s) 3865
XhoI CTCGAG 1 cut(s) 2969
XmiI GTMKAC 1 cut(s) 2535
XmnI GAANNNNTTC 1 cut(s) 2640
XspI CTAG 7 cut(s) 723, 860, 1118, 1379, 1679, 1778, 3693
ZraI GACGTC 2 cut(s) 2020, 2179
ZrmI AGTACT 1 cut(s) 1596
Zsp2I ATGCAT 3 cut(s) 709, 769, 4092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.