Rroxscaffold_4G00290920

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005782
Physical Location & Seq
Reverse (-)
11356146 .. 11364060
7915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00290920.1

Sequence Viewer

Length: 4146 bp
ATGATGATCAATCGGGGGCTGTTCGGGTGGTCCCCGCCGCACGTACAGCCGCTGACTCCGGTGTCTGAGGTCTCGGAGCCGCCGGAGTCGCCGTCGCCGTACATGGACATGAGCGGGGACACGTCGGCGCAGCCGGTCGAGCAGGAGGAGGAGATGGAGGAGCCTGAGGAGATCGAGCCGCCGCCCGCTGCCGTGCCGTTTTCGAGGCTGTTCACCTGCGCCGACCGCCTCGATTGGGTTTTGATGACCGTCGGATCGCTGGCGGCCGCCGCGCACGGCACGGCGCTCGTGGTGTACTTGCACTTTTTTGCTAAGATTATTCATGTGCTTTGGATCGATAGGGATTCCAAAGAACCGCCGCCGATGCATATGACGCCGGAGCAGTACCAGAAGTTTATGGATCTTGCTTTAAGCATTGTTTATATCGCTGTGGGTGTTTTTGCTGCTGGTTGGATTGAGGTCTCATGCTGGATTCTGACTGGAGAACGGCAGACTGCTGTCATCAGGTCAAAATATGTTCAAGTATTACTTAACCAGGATATGAGTTTTTTTGATACCTATGGGAACAATGGGGACATCGTGAGCCAAGTTTTGAGTGATGTTCTACTCATTCAGTCTGCTCTCAGTGAAAAAGTTGGAAATTATATTCATAACATGGCGACATTTTTCAGTGGGCTCATTATTGGATTCATCAACTGTTGGCAGATTGCAGCTATAACATTAGCCACTGGTCCATTTATTGTTGCTGCTGGAGGAATATCAAATATATTTCTTCACAGGCTTGCTGAGAATATTCAAGATGCATATGCTGAAGCAGCTAGCATTGCTGAACAGGCAGTCTCTTACATTGGGACATTATATGCATTTACAAATGAAACATTGGCCAAGTATTCTTATGCAACATCACTACAAGCAACTCTTCGATATGGTATATTGATTAGTCTGGTGCAAGGACTAGGACTTGGATTTACATATGGGCTTGCCATTTGTTCATGTGCCTTGCAACTCTGGGTTGGAAGGATCCTGGTTACTCATGGAAAAGCTCATGGTGGTGAAATTATAACAGCTTTATTTGCTGTAATATTAAGTGGCCTTGGGCTGAACCAAGCAGCAACAAACTTCTACTCATTTGATCAAGGTCGAATTGCGGCTTATAGACTGTTTGAGATGATACAAAGATCATCCTCTAATGTTAATCACGAGGGAACTACCCTAGTGGCTGTACAAGGAAATATTGAGTTCCGGAATGTGTATTTCAGTTATCCATCTCGTCCGGAGATTCCTATTTTGAGTAGTTTTTACCTCTCTGTACCTGCTAAGAAAGCTGTGGCACTCGTGGGTAGAAATGGATCTGGAAAGAGCAGTATCATTCCCCTCATGGAGCAGTTTTATAATCCAACATTAGGAGAAGTTCTTTTGGACGGAGAAAATATAAAAAACCTGAATTTGGAATGGCTCAGAAGCCAGATAGGGCTAGTCACACAAGATCCTGCCTTGTTAAGTTTGAGTATAAGAGATAATATTGCTTATGGGCGGGATGCGACAATGGATCAAATTGAAGAAGCTGCTAAAATAGCACATGCACATACATTTATTACTTCGCTTGAGAGATCGTATGAAACACAGGTAGGAAGGGCAGGATTGGCACTGACAGAAGAGCAAAAAATAAAACTTTCTATTGCCAGGGCAGTACTTCTGAATCCATCAATTCTTCTGCTTGATGAGGTCACTGGTGGACTTGATTTTGAAGCTGAAAGAACTGTTCAAGAGGCTCTAGATCTCCTCATGTTGGGACGCTCAACTATAATTATAGCTCGGCGGCTTAGTCTTATAAGGAATGCTGATTACATAGCTGTGATGGAGGAAGGTCAACTAGTTGAAACGGGTACACATGAAGAGTTAATAACCCATGATGGCCTATATGCAGAGCTTCTCAGGTGTGAAGAAGCTGCAAAGCTTCCCAGGAGAATGCCAGTGCGGAATTACAAGGAGACTACAGCTTTCCAAATTGAAATGGATTCTTCAGCAAGTAATAGCTTCCAAGAACCATCATCCCCAAAAATGATGAAATCACCCTCACTTCAGAGGGTTACCGGTATATTTCGAATGGGTGATGTCAATTTTAATGCACAGGACTCACCAAAACCTAAGAGTCCACCAGTAGAGAAAGTTGTGGAAAACGGTCAGGAGCCCTTGGATACAGCAGATAAGGAACCATCAATAAAGCGGCAGGATAGTTTTGAAATGAGACTACCAGAGCTACCGAAGATTGATGTCCAGTCTGCAAATCAACAAACAACAAATGGTTCGGACCCTGAATCCCCTGTTTCACCCCTTTTGACATCTGATCCGAAAAATGAACGTTCCCATTCACAAACCTTTAGCCGCCCTCATAGCCATTCTGATGATGTTCCCATCAAAGTGAAGGGAGCAAAGAGTACACGTTATAAAAAGTCACCATCATTTTGGAGGCTTGCACAGCTTTCTTTTGCAGAGTGGCTTTATGCTGTGTTAGGAAGCATTGGTGCTGCTATCTTTGGTTCCTTTAATCCTCTTCTTGCTTATGTTATCGCACTTGTAGTAACGGCATACTACAGGGGTGATGAAGGTCGTCACTTGAGGCAAGAAGTAGACAAATGGTGCTTGATTATTGCTTGCATGGGTATAGTGACAGTCGTTGCCAATTTCTTGCAACATTTCTACTTTGGTATCATGGGGGAGAAAATGACTGAACGAGTTCGCAGAATGATGTTTTCAGCAATGCTCCGCAATGAGGTGGGATGGTTTGATGAAGAGGAAAACAGTGCTGATACCTTGTCCATGCGCTTGGCAAATGATGCTACATTTGTGCGAGCTGCTTTTAGTAATCGGCTTTCAATATTCATACAGGATAGTGCTGCTATCATCGTGGCTGTTTTGATTGGGATGTTGCTACAATGGCGATTAGCACTTGTGGCTTTGGCAACCCTACCTGTTCTCACTATTTCTGCCATTGCACAGAAATTATGGCTTGCTGGTTTTTCAAGGGGGATTCAGGAGATGCACAGGAAAGCATCTTTGGTTCTGGAGGATGCAGTTAGAAATATTTACACTGTGGTAGCATTCTGTGCTGGTAACAAGGTAATGGAGCTCTACAGATTGCAGCTAAAGAAGATCTTTAAGCAGAGTTTCTTTCATGGAATGGCCATCGGGTTTGCATTTGGTTTTTCACAGTTCCTTCTTTTTGCCTGCAACGCTCTTCTCCTCTGGTACACTGCATACTCCGTGAAACAGAAGTATATGGATCTACCTACTGCTCTCAAGGAGTATATGGTTTTCTCTTTTGCAACATTCGCACTGGTAGAGCCCTTTGGGTTGGCTCCTTACATACTCAAACGACGTAAATCTCTCATTTCAGTATTTGAAATCATAGATCGAGTGCCAAAGATTGAGCCAGATGAAAGCTCTGCCATGAAACCACCTAATGTTTATGGAAGCATTGAGTTGAAAAATGTTGATTTCTGTTATCCAACCCGCCCAGAACTGTTGGTGCTGAGCAATTTCAGTCTCAAAGTAAATGGGGGGCAAACTGTAGCTGTGGTGGGAGTTTCAGGGTCGGGTAAGAGCACTATAATTTCTTTGATCGAGAGATTTTATGATCCAGTTGCTGGTCAAGTGATGCTGGATGGTCGAGATCTGAAGCTTTATAATTTGAGATGGTTGAGGAACCACCTTGGTCTTGTCCAACAGGAGCCCATTATTTTCTCAACAACAATAAGGGAGAATATAATATATGCAAGGCATAATGCTAGTGAAGCTGAAATGAAAGAGGCTGCAAGAATAGCGAATGCTCATCATTTCATCAGCAGCTTGCCTCATGGTTATGATACACATGTGGGGATGAGGGGGGTTGATCTAACCCCAGGACAGAAGCAAAGAATTGCAATCGCTCGTGTTGTTTTAAAAAATGCTCCCATTTTGTTATTGGATGAAGCAAGTTCGTCTATTGAATCTGAGTCAAGTCGAGTGGTGCAGGAGGCCCTTGATACATTAATCATGGGGAACAAAACAACCATTTTGATAGCTCACAGAGCTGCAATGATGAGGCACGTTGACAACATTGTAGTTCTTAATGGAGGGGAGAATTGTGGAAGAAGGGTCCCATGA

Protein Analysis

1381

Amino Acids

153.3

Weight (kDa)

6.0

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 86 - 362 1.1e-40 ABC transporter transmembrane region
ABC_tran PF00005 433 - 577 4.8e-22 ABC transporter
ABC_membrane PF00664 841 - 1100 1.1e-39 ABC transporter transmembrane region
ABC_tran PF00005 1178 - 1326 1.9e-28 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1061, 1392, 1832, 2448, 3687
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 61
Acc36I ACCTGC 2 cut(s) 224, 1321
AccB7I CCANNNNNTGG 1 cut(s) 3647
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 2631
AccII CGCG 1 cut(s) 272
AccIII TCCGGA 2 cut(s) 1242, 1273
AclI AACGTT 1 cut(s) 2362
AcoI YGGCCR 3 cut(s) 264, 882, 3183
AcsI RAATTY 1 cut(s) 1444
AcuI CTGAAG 4 cut(s) 831, 2007, 2066, 3698
AcyI GRCGYC 1 cut(s) 374
AfiI CCNNNNNNNGG 6 cut(s) 235, 1403, 1447, 1789, 2773, 3647
AflIII ACRYGT 3 cut(s) 120, 2441, 3871
AgeI ACCGGT 1 cut(s) 2093
AhlI ACTAGT 1 cut(s) 1873
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 5 cut(s) 534, 1023, 1682, 1961, 3901
AjuI GAANNNNNNNTTGG 2 cut(s) 3041, 3073
Alw21I GWGCWC 2 cut(s) 3132, 3608
Alw26I GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
AlwNI CAGNNNCTG 2 cut(s) 52, 3647
Aor13HI TCCGGA 2 cut(s) 1242, 1273
AoxI GGCC 6 cut(s) 264, 882, 1090, 1915, 3183, 4017
ApoI RAATTY 1 cut(s) 1444
AseI ATTAAT 1 cut(s) 4031
AsiGI ACCGGT 1 cut(s) 2093
Asp700I GAANNNNTTC 1 cut(s) 2736
AspLEI GCGC 5 cut(s) 130, 221, 274, 286, 2826
AspS9I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4138
AsuHPI GGTGA 8 cut(s) 205, 1064, 2064, 2123, 2130, 2322, 2448, 2612
AsuII TTCGAA 1 cut(s) 2104
AsuNHI GCTAGC 1 cut(s) 818
AvaII GGWCC 4 cut(s) 30, 731, 2311, 4138
AxyI CCTNAGG 1 cut(s) 165
BaeI ACNNNNGTAYC 4 cut(s) 2692, 2725, 3858, 3891
BalI TGGCCA 2 cut(s) 884, 3185
BamHI GGATCC 1 cut(s) 1020
BanII GRGCYC 5 cut(s) 678, 2193, 3132, 3348, 3735
BauI CACGAG 4 cut(s) 287, 1199, 1334, 3930
Bbv12I GWGCWC 2 cut(s) 3132, 3608
BceAI ACGGC 8 cut(s) 76, 82, 176, 181, 292, 297, 503, 2601
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 5 cut(s) 536, 1025, 1684, 1963, 3903
BciVI GTATCC 1 cut(s) 2191
BclI TGATCA 2 cut(s) 6, 1132
BcoDI GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
BcuI ACTAGT 1 cut(s) 1873
BfaI CTAG 7 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3789
BfmI CTRYAG 4 cut(s) 1995, 2593, 3133, 3570
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 2 cut(s) 224, 1321
BfuI GTATCC 1 cut(s) 2191
BglII AGATCT 3 cut(s) 1777, 3153, 3673
BlpI GCTNAGC 1 cut(s) 3533
BmcAI AGTACT 1 cut(s) 1692
Bme1390I CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
Bme18I GGWCC 4 cut(s) 30, 731, 2311, 4138
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4138
BmrFI CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
BmsI GCATC 8 cut(s) 354, 790, 1528, 2827, 3030, 3061, 3062, 3648
BmtI GCTAGC 1 cut(s) 822
BoxI GACNNNNGTC 1 cut(s) 497
BpmI CTGGAG 3 cut(s) 501, 771, 3086
Bpu1102I GCTNAGC 1 cut(s) 3533
Bpu14I TTCGAA 1 cut(s) 2104
BpuEI CTTGAG 3 cut(s) 1625, 2638, 3284
Bsa29I ATCGAT 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 374
BsaI GGTCTC 2 cut(s) 76, 466
BsaJI CCNNGG 6 cut(s) 1093, 1683, 1961, 2193, 3712, 3901
BsaWI WCCGGW 4 cut(s) 58, 1242, 1273, 2093
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 6 cut(s) 235, 1403, 1447, 1789, 2773, 3647
Bse118I RCCGGY 2 cut(s) 133, 2093
Bse1I ACTGG 8 cut(s) 484, 733, 1735, 1973, 2159, 2278, 3342, 3641
Bse21I CCTNAGG 1 cut(s) 165
Bse3DI GCAATG 5 cut(s) 822, 2766, 2776, 2991, 4083
BseAI TCCGGA 2 cut(s) 1242, 1273
BseBI CCWGG 5 cut(s) 536, 1025, 1684, 1963, 3903
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 6 cut(s) 1093, 1683, 1961, 2193, 3712, 3901
BseGI GGATG 9 cut(s) 1181, 1543, 2051, 2786, 2931, 3076, 3670, 3885, 3973
BseLI CCNNNNNNNGG 6 cut(s) 235, 1403, 1447, 1789, 2773, 3647
BseMI GCAATG 5 cut(s) 822, 2766, 2776, 2991, 4083
BseMII CTCAG 8 cut(s) 57, 156, 637, 777, 1471, 1948, 3524, 3984
BseNI ACTGG 8 cut(s) 484, 733, 1735, 1973, 2159, 2278, 3342, 3641
BseRI GAGGAG 6 cut(s) 161, 164, 173, 182, 1772, 3233
BseX3I CGGCCG 1 cut(s) 264
BsgI GTGCAG 1 cut(s) 4031
Bsh1236I CGCG 1 cut(s) 272
Bsh1285I CGRYCG 3 cut(s) 138, 226, 267
BshFI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
BshTI ACCGGT 1 cut(s) 2093
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 3 cut(s) 138, 226, 267
BsiHKAI GWGCWC 2 cut(s) 3132, 3608
BsiSI CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 2094
BslFI GGGAC 6 cut(s) 16, 131, 587, 865, 1806, 4124
BslI CCNNNNNNNGG 6 cut(s) 235, 1403, 1447, 1789, 2773, 3647
BsmAI GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
BsmFI GGGAC 6 cut(s) 16, 131, 587, 865, 1806, 4124
BsmI GAATGC 4 cut(s) 1843, 1974, 3101, 3832
BsnI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
Bso31I GGTCTC 2 cut(s) 76, 466
Bsp119I TTCGAA 1 cut(s) 2104
Bsp1286I GDGCHC 6 cut(s) 678, 2193, 3132, 3348, 3608, 3735
Bsp13I TCCGGA 2 cut(s) 1242, 1273
Bsp1407I TGTACA 1 cut(s) 1222
Bsp1720I GCTNAGC 1 cut(s) 3533
BspANI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
BspCNI CTCAG 8 cut(s) 58, 157, 636, 778, 1470, 1947, 3525, 3985
BspDI ATCGAT 1 cut(s) 336
BspEI TCCGGA 2 cut(s) 1242, 1273
BspFNI CGCG 1 cut(s) 272
BspMI ACCTGC 2 cut(s) 224, 1321
BspOI GCTAGC 1 cut(s) 822
BspQI GCTCTTC 2 cut(s) 1650, 3243
BspT104I TTCGAA 1 cut(s) 2104
BspTNI GGTCTC 2 cut(s) 76, 466
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 5 cut(s) 822, 2766, 2776, 2991, 4083
BsrFI RCCGGY 2 cut(s) 133, 2093
BsrGI TGTACA 1 cut(s) 1222
BsrI ACTGG 8 cut(s) 484, 733, 1735, 1973, 2159, 2278, 3342, 3641
BssAI RCCGGY 2 cut(s) 133, 2093
BssECI CCNNGG 6 cut(s) 1093, 1683, 1961, 2193, 3712, 3901
BssNI GRCGYC 1 cut(s) 374
BssSI CACGAG 4 cut(s) 287, 1199, 1334, 3930
BssT1I CCWWGG 3 cut(s) 1093, 2193, 3712
Bst2BI CACGAG 4 cut(s) 287, 1199, 1334, 3930
Bst2UI CCWGG 5 cut(s) 536, 1025, 1684, 1963, 3903
Bst6I CTCTTC 6 cut(s) 924, 1650, 1890, 2559, 2787, 3243
BstACI GRCGYC 1 cut(s) 374
BstAPI GCANNNNNTGC 2 cut(s) 2837, 3107
BstAUI TGTACA 1 cut(s) 1222
BstBAI YACGTR 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 2104
BstEII GGTNACC 1 cut(s) 2089
BstF5I GGATG 9 cut(s) 1181, 1543, 2051, 2786, 2931, 3076, 3670, 3885, 3973
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 5 cut(s) 130, 221, 274, 286, 2826
BstMAI GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
BstMCI CGRYCG 3 cut(s) 138, 226, 267
BstNI CCWGG 5 cut(s) 536, 1025, 1684, 1963, 3903
BstNSI RCATGY 2 cut(s) 1583, 3875
BstPAI GACNNNNGTC 1 cut(s) 497
BstPI GGTNACC 1 cut(s) 2089
BstSCI CCNGG 5 cut(s) 534, 1023, 1682, 1961, 3901
BstSFI CTRYAG 4 cut(s) 1995, 2593, 3133, 3570
BstUI CGCG 1 cut(s) 272
BstX2I RGATCY 8 cut(s) 400, 1020, 1349, 1486, 1777, 3153, 3283, 3673
BstXI CCANNNNNNTGG 2 cut(s) 2466, 2827
BstYI RGATCY 8 cut(s) 400, 1020, 1349, 1486, 1777, 3153, 3283, 3673
BstZI CGGCCG 1 cut(s) 264
Bsu15I ATCGAT 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 165
BsuI GTATCC 1 cut(s) 2191
BsuRI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
BsuTUI ATCGAT 1 cut(s) 336
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 9 cut(s) 1181, 1543, 2051, 2786, 2931, 3076, 3670, 3885, 3973
BtsI GCAGTG 1 cut(s) 3252
BveI ACCTGC 2 cut(s) 224, 1321
CaiI CAGNNNCTG 2 cut(s) 52, 3647
CciNI GCGGCCGC 1 cut(s) 264
CfoI GCGC 5 cut(s) 130, 221, 274, 286, 2826
Cfr10I RCCGGY 2 cut(s) 133, 2093
Cfr13I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4138
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 2 cut(s) 382, 1803
CspAI ACCGGT 1 cut(s) 2093
CspCI CAANNNNNGTGG 2 cut(s) 3987, 4022
DraI TTTAAA 1 cut(s) 3942
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 3 cut(s) 264, 882, 3183
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 6 cut(s) 924, 1650, 1890, 2559, 2787, 3243
EarI CTCTTC 6 cut(s) 924, 1650, 1890, 2559, 2787, 3243
Ecl136II GAGCTC 1 cut(s) 3130
EclXI CGGCCG 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 1093, 2193, 3712
Eco24I GRGCYC 5 cut(s) 678, 2193, 3132, 3348, 3735
Eco31I GGTCTC 2 cut(s) 76, 466
Eco47I GGWCC 4 cut(s) 30, 731, 2311, 4138
Eco52I CGGCCG 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 3130
Eco57I CTGAAG 4 cut(s) 831, 2007, 2066, 3698
Eco81I CCTNAGG 1 cut(s) 165
Eco91I GGTNACC 1 cut(s) 2089
EcoICRI GAGCTC 1 cut(s) 3130
EcoO109I RGGNCCY 2 cut(s) 4018, 4138
EcoO65I GGTNACC 1 cut(s) 2089
EcoRII CCWGG 5 cut(s) 534, 1023, 1682, 1961, 3901
EcoT14I CCWWGG 3 cut(s) 1093, 2193, 3712
EcoT22I ATGCAT 3 cut(s) 369, 805, 865
EcoT38I GRGCYC 5 cut(s) 678, 2193, 3132, 3348, 3735
ErhI CCWWGG 3 cut(s) 1093, 2193, 3712
FalI AAGNNNNNCTT 6 cut(s) 513, 545, 903, 935, 3140, 3172
FaqI GGGAC 6 cut(s) 16, 131, 587, 865, 1806, 4124
FauI CCCGC 5 cut(s) 42, 107, 193, 1527, 3521
FauNDI CATATG 3 cut(s) 369, 805, 973
FbaI TGATCA 2 cut(s) 6, 1132
FblI GTMKAC 1 cut(s) 2631
FokI GGATG 9 cut(s) 1168, 1550, 2038, 2793, 2938, 3083, 3677, 3892, 3980
FriOI GRGCYC 5 cut(s) 678, 2193, 3132, 3348, 3735
FspBI CTAG 7 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3789
GlaI GCGC 5 cut(s) 129, 220, 273, 285, 2825
GsuI CTGGAG 3 cut(s) 501, 771, 3086
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
HapII CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 2094
HgaI GACGC 2 cut(s) 382, 1803
HhaI GCGC 5 cut(s) 130, 221, 274, 286, 2826
Hin1I GRCGYC 1 cut(s) 374
Hin6I GCGC 5 cut(s) 128, 219, 272, 284, 2824
HinP1I GCGC 5 cut(s) 128, 219, 272, 284, 2824
HincII GTYRAC 2 cut(s) 1871, 4093
HindII GTYRAC 2 cut(s) 1871, 4093
HindIII AAGCTT 2 cut(s) 1955, 3680
HpaII CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 2094
HphI GGTGA 8 cut(s) 205, 1064, 2064, 2123, 2130, 2322, 2448, 2612
Hpy99I CGWCG 4 cut(s) 97, 127, 254, 3381
HpyAV CCTTC 7 cut(s) 1011, 1626, 1859, 2419, 2600, 3227, 4128
HpyCH4IV ACGT 6 cut(s) 42, 122, 2362, 2443, 3379, 4089
HpySE526I ACGT 6 cut(s) 42, 122, 2362, 2443, 3379, 4089
Hsp92I GRCGYC 1 cut(s) 374
HspAI GCGC 5 cut(s) 128, 219, 272, 284, 2824
KflI GGGWCCC 1 cut(s) 4138
Kpn2I TCCGGA 2 cut(s) 1242, 1273
Ksp22I TGATCA 2 cut(s) 6, 1132
LguI GCTCTTC 2 cut(s) 1650, 3243
LweI GCATC 8 cut(s) 354, 790, 1528, 2827, 3030, 3061, 3062, 3648
MaeI CTAG 7 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3789
MaeII ACGT 6 cut(s) 42, 122, 2362, 2443, 3379, 4089
MaeIII GTNAC 9 cut(s) 1027, 1477, 1726, 2089, 2454, 2581, 2612, 2668, 3113
MbiI CCGCTC 1 cut(s) 114
MflI RGATCY 8 cut(s) 400, 1020, 1349, 1486, 1777, 3153, 3283, 3673
MhlI GDGCHC 6 cut(s) 678, 2193, 3132, 3348, 3608, 3735
MlsI TGGCCA 2 cut(s) 884, 3185
MluNI TGGCCA 2 cut(s) 884, 3185
MlyI GAGTC 5 cut(s) 49, 95, 2129, 2161, 4004
MmeI TCCRAC 7 cut(s) 232, 431, 616, 994, 1421, 3533, 3748
Mox20I TGGCCA 2 cut(s) 884, 3185
Mph1103I ATGCAT 3 cut(s) 369, 805, 865
MroI TCCGGA 2 cut(s) 1242, 1273
MroXI GAANNNNTTC 1 cut(s) 2736
MscI TGGCCA 2 cut(s) 884, 3185
MslI CAYNNNNRTG 6 cut(s) 107, 1050, 1853, 2403, 2420, 3861
Msp20I TGGCCA 2 cut(s) 884, 3185
MspA1I CMGCKG 2 cut(s) 52, 188
MspI CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 2094
MspR9I CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
Mva1269I GAATGC 4 cut(s) 1843, 1974, 3101, 3832
MvaI CCWGG 5 cut(s) 536, 1025, 1684, 1963, 3903
MvnI CGCG 1 cut(s) 272
NdeI CATATG 3 cut(s) 369, 805, 973
NheI GCTAGC 1 cut(s) 818
NmeAIII GCCGAG 1 cut(s) 1795
NmuCI GTSAC 5 cut(s) 1477, 1726, 2454, 2612, 2668
NotI GCGGCCGC 1 cut(s) 264
NsiI ATGCAT 3 cut(s) 369, 805, 865
NspI RCATGY 2 cut(s) 1583, 3875
NspV TTCGAA 1 cut(s) 2104
PaqCI CACCTGC 1 cut(s) 224
PciI ACATGT 1 cut(s) 3871
PciSI GCTCTTC 2 cut(s) 1650, 3243
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PctI GAATGC 4 cut(s) 1843, 1974, 3101, 3832
PdmI GAANNNNTTC 1 cut(s) 2736
PfeI GAWTC 9 cut(s) 344, 472, 687, 1279, 1699, 2018, 2318, 3031, 3989
PflMI CCANNNNNTGG 1 cut(s) 3647
PinAI ACCGGT 1 cut(s) 2093
PleI GAGTC 5 cut(s) 49, 94, 2129, 2160, 4003
PpsI GAGTC 5 cut(s) 49, 94, 2129, 2160, 4003
Ppu21I YACGTR 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 4138
PscI ACATGT 1 cut(s) 3871
PshAI GACNNNNGTC 1 cut(s) 497
PshBI ATTAAT 1 cut(s) 4031
PsiI TTATAA 5 cut(s) 1061, 1392, 1832, 2448, 3687
Psp124BI GAGCTC 1 cut(s) 3132
Psp1406I AACGTT 1 cut(s) 2362
Psp5II RGGWCCY 1 cut(s) 4138
Psp6I CCWGG 5 cut(s) 534, 1023, 1682, 1961, 3901
PspEI GGTNACC 1 cut(s) 2089
PspGI CCWGG 5 cut(s) 534, 1023, 1682, 1961, 3901
PspPI GGNCC 5 cut(s) 30, 731, 2311, 4018, 4138
PspPPI RGGWCCY 1 cut(s) 4138
PstNI CAGNNNCTG 2 cut(s) 52, 3647
PsuI RGATCY 8 cut(s) 400, 1020, 1349, 1486, 1777, 3153, 3283, 3673
RseI CAYNNNNRTG 6 cut(s) 107, 1050, 1853, 2403, 2420, 3861
SacI GAGCTC 1 cut(s) 3132
SapI GCTCTTC 2 cut(s) 1650, 3243
Sau96I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4138
ScaI AGTACT 1 cut(s) 1692
SchI GAGTC 5 cut(s) 49, 95, 2129, 2161, 4004
ScrFI CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
SduI GDGCHC 6 cut(s) 678, 2193, 3132, 3348, 3608, 3735
SfaNI GCATC 8 cut(s) 354, 790, 1528, 2827, 3030, 3061, 3062, 3648
SfcI CTRYAG 4 cut(s) 1995, 2593, 3133, 3570
SfuI TTCGAA 1 cut(s) 2104
SinI GGWCC 4 cut(s) 30, 731, 2311, 4138
SmiMI CAYNNNNRTG 6 cut(s) 107, 1050, 1853, 2403, 2420, 3861
SmlI CTYRAG 3 cut(s) 1604, 2617, 3299
SmoI CTYRAG 3 cut(s) 1604, 2617, 3299
SpeI ACTAGT 1 cut(s) 1873
SspI AATATT 6 cut(s) 793, 1083, 1234, 1522, 2880, 3085
SspMI CTAG 7 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3789
SstI GAGCTC 1 cut(s) 3132
StyD4I CCNGG 5 cut(s) 534, 1023, 1682, 1961, 3901
StyI CCWWGG 3 cut(s) 1093, 2193, 3712
TaiI ACGT 6 cut(s) 45, 125, 2365, 2446, 3382, 4092
TatI WGTACW 4 cut(s) 294, 1222, 1690, 2438
TfiI GAWTC 9 cut(s) 344, 472, 687, 1279, 1699, 2018, 2318, 3031, 3989
TseFI GTSAC 5 cut(s) 1477, 1726, 2454, 2612, 2668
Tsp45I GTSAC 5 cut(s) 1477, 1726, 2454, 2612, 2668
TspGWI ACGGA 2 cut(s) 1437, 3253
Van91I CCANNNNNTGG 1 cut(s) 3647
VpaK11BI GGWCC 4 cut(s) 30, 731, 2311, 4138
VspI ATTAAT 1 cut(s) 4031
XapI RAATTY 1 cut(s) 1444
XbaI TCTAGA 1 cut(s) 1774
XceI RCATGY 2 cut(s) 1583, 3875
XcmI CCANNNNNNNNNTGG 1 cut(s) 3961
XmiI GTMKAC 1 cut(s) 2631
XmnI GAANNNNTTC 1 cut(s) 2736
XspI CTAG 7 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3789
ZrmI AGTACT 1 cut(s) 1692
Zsp2I ATGCAT 3 cut(s) 369, 805, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.