Rh1AG332700

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005782
Physical Location & Seq
Forward (+)
56780037 .. 56787936
7900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG332700.1

Sequence Viewer

Length: 3576 bp
ATGGCGACATTTTTCAGTGGGCTCATTATTGGATTCATCAACTGTTGGCAGATTGCGGCTATAACATTAGCCACTGGTCCATTTATTGTTGCTGCTGGAGGAATATCAAATATATTTCTTCACAGGCTTGCTGAGAATATTCAAGATGCATATGCTGAAGCAGCTAGCATTGCTGAACAGGCAGTCTCTTACATTGGGACATTATATGCATTTACAAATGAAACATTGGCCAAGTATTCTTATGCAACATCACTACAAGCAACTCTTCGATATGGTATATTGATTAGTCTGGTGCAAGGACTAGGACTTGGATTTACATATGGGCTTGCCATTTGTTCATGTGCCTTGCAACTCTGGGTTGGAAGGTTCCTGGTTACTGATCGAAAAGCTCATGGTGGTGAAATTATAACAGCTTTATTTGCTGTAATATTAAGTGGCCTTGGGCTGAACCAAGCAGCAACAAACTTCTACTCATTTGATCAAGGTCGAATTGCGGCTTATAGACTGTTTGAGATGATACAAAGATCATCCTCTAATGTAAATCATGAGGGAACTATCCTAGTGGCTGTACAAGGAAATATTGAGTTCCGGAATGTGTATTTCAGTTATCCATCTCGTCCGGAGATTCCTATTTTAAGTAGTTTTTACCTCTCTGTACCTGCTAAGAAAGCTGTGGCACTCGTGGGTAGAAATGGATCTGGAAAGAGCAGTATCATTCCCCTCATGGAGCAGTTTTATAATCCAACATTAGGAGAAGTTCTTTTGGATGGAGAAAATATAAAAAACTTGAATTTGGAATGGCTCAGAAGCCAGATAGGGCTAGTCACACAAGATCCCGCCTTGTTAAGTTTGAGTATAAGAGATAATATTGCTTATGGGCGGGATGCGACAATGGATCAAATTGAAGAAGCTGCTAAAATAGCACATGCGCATACATTTATTACTTCGCTTGAGAGATCGTATGAAACACAGGTAGGAAGGGCAGGATTAGCACTGACAGAAGAGCAAAAAATAAAACTTTCAATTGCCAGGGCAGTACTTCTGAATCCATCAATTCTTCTGCTTGATGAGGTCACTGGTGGACTTGATTTTGAAGCTGAAAGAACTGTTCAAGAGGCTCTAGATCTCCTCATGTTGGGACGTTCAACTATAATTATAGCTCGGCGGCTTAGTCTTATAAGGAATGCTGATTACATAGCTGTGATGGAGGAAGGTCAACTAGTTGAAACGGGTACACATGAAGAGTTAATAACCCATGATGGCCTATATGCAGAGCTTCTCAGGTGTGAAGAAGCTGCAAAGCTTCCCAGGAGAATGCCAGCGCGGAATTACAAGGAGACTACAGCTTTCCAAATTGAAATGGATTCTTCAGCAAGTAATAGCTTCCAAGAACCATCATCCCCAAAAATGATGAAATCACCCTCACTTCAGAGGGTTACTGGTATATTTCGAATGGGTGACGTCAATTTTAATGCACAGGACTCACCAAAACCTAAGAGTCCACCAGTAGAGAAAGTGGTGGAAAACGGTCAGCAGCCCTTGGATACAGCAGATAAGGAACCATCAATAAAGCGGCAGGATAGTTTTGAAATGAGACTACCAGAGCTACCCAAGATTGATGTCCAGTCTGCAAATCAACAAACAACAAATGGTTCGGACCCTGAATCCCCTGTTTCACCCCTTTTGACATCTGATCCGAAAAATGAACGTTCCCATTCACAAACCTTTAGCCGCCCTCATAGCCATTCTGATGATGTTCCCATCAAAGTGAAGGGAGCAAAGAGTACACATTATAAAAAGTCACCATCATTTTGGAGGCTTGCACAGCTTTCTTTTGCAGAGTGGCTTTATGCTGTGTTAGGAAGCATTGGTGCTGCTATCTTTGGTTCCTTTAATCCTCTTCTTGCTTATGTTATCGCACTTGTAGTAACAGCATACTACAGGGGTGATGAAGGTCGTCACTTGAGGCAAGAAGTAGACAAATGGTGCTTGATCATTGCTTGCATGGGTATAGTGACAGTCGTTGCCAATTTCTTGCAACACTTCTACTTTGGTATCATGGGGGAGAAAATGACTGAACGAGTTCGCAGAATGATGTTTTCAGCAATGCTCCGTAATGAGGTGGGATGGTTTGATGAAGAGGAAAACAGTGCTGATACTTTGTCCATGCGCTTGGCAAATGATGCTACATTTGTGCGAGCTGCTTTTAGTAATCGGCTTTCAATATTCATACAGGATAGTGCTGCTATCATTGTGGCTGTTTTGATTGGGATGTTGCTACAATGGCGATTAGCACTTGTGGCTTTGGCAACCCTACCTGTTCTCACTATTTCTGCCATTGCACAGAAATTATGGCTTGCTGGTTTTTCAAGGGGGATTCAGGAGATGCACAGGAAAGCATCTTTGGTTCTCGAGGATGCAGTTAGAAACATTTACACTGTGGTAGCATTCTGTGCTGGTAACAAGGTAATGGAGCTCTACAGATTGCAGCTAAAGAAGATCTTTAAGCAGAGTTTCTTTCATGGAATGGCCATTGGGTTTGCATTTGGTTTTTCACAGTTCCTTCTTTTTGCCTGCAATGCTCTTCTCCTCTGGTACACTGCATACTCCGTGAAACAGGAGTATATGAATCTACCTACTGCTCTCAAGGAGTATATGGTTTTCTCTTTTGCAACATTCGCACTGGTAGAGCCCTTTGGGTTGGCTCCTTACATACTCAAACGACGTAAATCTCTCATTTCGGTATTTGAAATCATAGATCGAGTGCCAAAGATTGAGCCAGATGAAAGCTCCGCCATGAAACCACCTAATGTTTATGGAAGCCTTGAGTTGAAAAATGTTGATTTCTGTTATCCAACCCGCCCGGAACTGTTGGTGCTGAGCAATTTCAGTCTCAAAGTAAACGGGGGTCAAACTGTAGCTGTGGTGGGAGTTTCAGGGTCGGGTAAGAGCACTATAATTTCTTTGATCGAGAGATTTTATGATCCAGTTGCTGGTCAAGTGATGCTGGATGGTCGAGATCTGAAGCTTTATAATTTGAGATGGTTGAGGAACCACCTTGGTCTTGTTCAACAGGAGCCCATTATTTTCTCGACAACAATAAGGGAGAATATAATATATGCAAGGCATAATGCTAGTGAAGCTGAAATGAAAGAGGCTGCAAGAATAGCGAATGCTCATCATTTCATCAGCAGCTTGCCTCATGGTTATGATACACATGTGGGGATGAGAGGGGTTGATCTAACCCCAGGACAGAAGCAAAGAATTGCAATCGCTCGTGTTGTTTTAAAAAATGCTCCCATTTTGTTATTGGACGAAGCAAGTTCGTCTATTGAATCTGAGTCAAGTCGAGTGGTGCAGGAGGCCCTTGATACATTAATCATGGGGAACAAAACAACCATCTTGATAGCTCACAGAGCTGCGATGATGAGGCATGTTGACAACATTGTAGTTCTTAATGGAGGGAGAATTGTGGAAGAAGGGTCCCATGATTCTTTAATGTCAAAGAACGGTCTGTATGTCCGTTTGATGCATCCTCACTTTGGCAAGGGCCTGCGTCAGCACCGCCTTGTTTAG

Protein Analysis

1191

Amino Acids

132.32

Weight (kDa)

7.66

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 2 - 144 5.6e-16 ABC transporter transmembrane region
ABC_tran PF00005 215 - 359 3.9e-22 ABC transporter
ABC_membrane PF00664 623 - 885 8.8e-40 ABC transporter transmembrane region
ABC_tran PF00005 960 - 1108 1.5e-28 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 407, 738, 1178, 1794, 3033
AatII GACGTC 1 cut(s) 1464
Acc16I TGCGCA 1 cut(s) 930
Acc36I ACCTGC 1 cut(s) 667
AccB7I CCANNNNNTGG 1 cut(s) 2993
AccI GTMKAC 1 cut(s) 1977
AccII CGCG 1 cut(s) 1324
AccIII TCCGGA 2 cut(s) 588, 619
AclI AACGTT 1 cut(s) 1708
AclWI GGATC 5 cut(s) 703, 827, 903, 1688, 2978
AcoI YGGCCR 2 cut(s) 228, 2529
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 4 cut(s) 177, 1353, 1412, 3044
AcyI GRCGYC 1 cut(s) 1461
AfaI GTAC 6 cut(s) 570, 657, 1038, 1234, 1786, 2597
AfiI CCNNNNNNNGG 5 cut(s) 749, 1135, 2119, 2993, 3542
AflIII ACRYGT 1 cut(s) 3217
AhlI ACTAGT 1 cut(s) 1219
AjnI CCWGG 4 cut(s) 369, 1028, 1307, 3247
AjuI GAANNNNNNNTTGG 2 cut(s) 2387, 2419
Alw21I GWGCWC 2 cut(s) 2478, 2954
Alw26I GTCTC 4 cut(s) 190, 1331, 1588, 2897
AlwI GGATC 5 cut(s) 703, 827, 903, 1688, 2978
AlwNI CAGNNNCTG 1 cut(s) 2993
Ama87I CYCGRG 1 cut(s) 2411
Aor13HI TCCGGA 2 cut(s) 588, 619
AoxI GGCC 6 cut(s) 228, 436, 1261, 2529, 3363, 3550
ApoI RAATTY 1 cut(s) 790
AseI ATTAAT 1 cut(s) 3377
Asp700I GAANNNNTTC 1 cut(s) 2082
AspLEI GCGC 3 cut(s) 931, 1324, 2172
AspS9I GGNCC 5 cut(s) 77, 1657, 3364, 3483, 3550
AsuC2I CCSGG 1 cut(s) 2864
AsuHPI GGTGA 7 cut(s) 410, 1410, 1469, 1476, 1668, 1794, 1958
AsuII TTCGAA 1 cut(s) 1450
AsuNHI GCTAGC 1 cut(s) 164
AvaI CYCGRG 1 cut(s) 2411
AvaII GGWCC 3 cut(s) 77, 1657, 3483
BaeI ACNNNNGTAYC 4 cut(s) 2038, 2071, 3204, 3237
BalI TGGCCA 2 cut(s) 230, 2531
BanII GRGCYC 4 cut(s) 24, 2478, 2694, 3081
BauI CACGAG 2 cut(s) 680, 3276
Bbv12I GWGCWC 2 cut(s) 2478, 2954
BciT130I CCWGG 4 cut(s) 371, 1030, 1309, 3249
BciVI GTATCC 1 cut(s) 1537
BclI TGATCA 2 cut(s) 478, 1992
BcnI CCSGG 1 cut(s) 2864
BcoDI GTCTC 4 cut(s) 190, 1331, 1588, 2897
BcuI ACTAGT 1 cut(s) 1219
BfaI CTAG 7 cut(s) 165, 302, 560, 821, 1121, 1220, 3135
BfmI CTRYAG 4 cut(s) 1341, 1939, 2479, 2916
BfuAI ACCTGC 1 cut(s) 667
BfuI GTATCC 1 cut(s) 1537
BglII AGATCT 3 cut(s) 1123, 2499, 3019
BlpI GCTNAGC 1 cut(s) 2879
BmcAI AGTACT 1 cut(s) 1038
Bme1390I CCNGG 5 cut(s) 371, 1030, 1309, 2864, 3249
Bme18I GGWCC 3 cut(s) 77, 1657, 3483
BmeT110I CYCGRG 1 cut(s) 2411
BmgT120I GGNCC 5 cut(s) 77, 1657, 3364, 3483, 3550
BmiI GGNNCC 9 cut(s) 368, 1560, 1659, 1888, 2706, 3053, 3078, 3484, 3485
BmrFI CCNGG 5 cut(s) 371, 1030, 1309, 2864, 3249
BmsI GCATC 9 cut(s) 136, 874, 2173, 2376, 2407, 2408, 2994, 3519, 3541
BmtI GCTAGC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 2879
Bpu14I TTCGAA 1 cut(s) 1450
BpuEI CTTGAG 4 cut(s) 971, 1984, 2630, 2846
BpuMI CCSGG 1 cut(s) 2864
BsaHI GRCGYC 1 cut(s) 1461
BsaJI CCNNGG 6 cut(s) 439, 1029, 1307, 1539, 3058, 3247
BsaWI WCCGGW 2 cut(s) 588, 619
Bsc4I CCNNNNNNNGG 5 cut(s) 749, 1135, 2119, 2993, 3542
Bse1I ACTGG 7 cut(s) 79, 1081, 1444, 1505, 1624, 2688, 2987
Bse3DI GCAATG 5 cut(s) 168, 1995, 2112, 2337, 2584
BseAI TCCGGA 2 cut(s) 588, 619
BseBI CCWGG 4 cut(s) 371, 1030, 1309, 3249
BseDI CCNNGG 6 cut(s) 439, 1029, 1307, 1539, 3058, 3247
BseLI CCNNNNNNNGG 5 cut(s) 749, 1135, 2119, 2993, 3542
BseMI GCAATG 5 cut(s) 168, 1995, 2112, 2337, 2584
BseMII CTCAG 5 cut(s) 123, 817, 1294, 2870, 3330
BseNI ACTGG 7 cut(s) 79, 1081, 1444, 1505, 1624, 2688, 2987
BseRI GAGGAG 2 cut(s) 1118, 2579
BsgI GTGCAG 1 cut(s) 3377
Bsh1236I CGCG 1 cut(s) 1324
BshFI GGCC 6 cut(s) 230, 438, 1263, 2531, 3365, 3552
BsiHKAI GWGCWC 2 cut(s) 2478, 2954
BsiHKCI CYCGRG 1 cut(s) 2411
BsiSI CCGG 3 cut(s) 589, 620, 2864
BslFI GGGAC 3 cut(s) 211, 1152, 3469
BslI CCNNNNNNNGG 5 cut(s) 749, 1135, 2119, 2993, 3542
BsmAI GTCTC 4 cut(s) 190, 1331, 1588, 2897
BsmFI GGGAC 3 cut(s) 211, 1152, 3469
BsmI GAATGC 4 cut(s) 1189, 1320, 2447, 3178
BsnI GGCC 6 cut(s) 230, 438, 1263, 2531, 3365, 3552
BsoBI CYCGRG 1 cut(s) 2411
Bsp119I TTCGAA 1 cut(s) 1450
Bsp1286I GDGCHC 5 cut(s) 24, 2478, 2694, 2954, 3081
Bsp13I TCCGGA 2 cut(s) 588, 619
Bsp1407I TGTACA 1 cut(s) 568
Bsp1720I GCTNAGC 1 cut(s) 2879
BspANI GGCC 6 cut(s) 230, 438, 1263, 2531, 3365, 3552
BspCNI CTCAG 5 cut(s) 124, 816, 1293, 2871, 3331
BspEI TCCGGA 2 cut(s) 588, 619
BspFNI CGCG 1 cut(s) 1324
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 9 cut(s) 368, 1560, 1659, 1888, 2706, 3053, 3078, 3484, 3485
BspMI ACCTGC 1 cut(s) 667
BspOI GCTAGC 1 cut(s) 168
BspPI GGATC 5 cut(s) 703, 827, 903, 1688, 2978
BspQI GCTCTTC 2 cut(s) 996, 2589
BspT104I TTCGAA 1 cut(s) 1450
BsrDI GCAATG 5 cut(s) 168, 1995, 2112, 2337, 2584
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 7 cut(s) 79, 1081, 1444, 1505, 1624, 2688, 2987
BssECI CCNNGG 6 cut(s) 439, 1029, 1307, 1539, 3058, 3247
BssNI GRCGYC 1 cut(s) 1461
BssSI CACGAG 2 cut(s) 680, 3276
BssT1I CCWWGG 3 cut(s) 439, 1539, 3058
Bst2BI CACGAG 2 cut(s) 680, 3276
Bst2UI CCWGG 4 cut(s) 371, 1030, 1309, 3249
Bst6I CTCTTC 6 cut(s) 270, 996, 1236, 1905, 2133, 2589
BstACI GRCGYC 1 cut(s) 1461
BstAPI GCANNNNNTGC 2 cut(s) 2183, 2453
BstAUI TGTACA 1 cut(s) 568
BstBI TTCGAA 1 cut(s) 1450
BstDEI CTNAG 8 cut(s) 132, 663, 803, 1169, 1280, 1494, 2879, 3339
BstFNI CGCG 1 cut(s) 1324
BstHHI GCGC 3 cut(s) 931, 1324, 2172
BstMAI GTCTC 4 cut(s) 190, 1331, 1588, 2897
BstNI CCWGG 4 cut(s) 371, 1030, 1309, 3249
BstNSI RCATGY 3 cut(s) 929, 3221, 3437
BstSCI CCNGG 5 cut(s) 369, 1028, 1307, 2862, 3247
BstSFI CTRYAG 4 cut(s) 1341, 1939, 2479, 2916
BstUI CGCG 1 cut(s) 1324
BstX2I RGATCY 5 cut(s) 695, 832, 1123, 2499, 3019
BstXI CCANNNNNNTGG 2 cut(s) 1812, 2173
BstYI RGATCY 5 cut(s) 695, 832, 1123, 2499, 3019
BsuI GTATCC 1 cut(s) 1537
BsuRI GGCC 6 cut(s) 230, 438, 1263, 2531, 3365, 3552
BtgZI GCGATG 1 cut(s) 3437
BtsI GCAGTG 1 cut(s) 2598
BtsIMutI CAGTG 8 cut(s) 22, 72, 992, 1074, 2155, 2436, 2598, 2681
BveI ACCTGC 1 cut(s) 667
CaiI CAGNNNCTG 1 cut(s) 2993
CciI TCATGA 1 cut(s) 544
CfoI GCGC 3 cut(s) 931, 1324, 2172
Cfr13I GGNCC 5 cut(s) 77, 1657, 3364, 3483, 3550
CseI GACGC 1 cut(s) 3545
Csp6I GTAC 6 cut(s) 569, 656, 1037, 1233, 1785, 2596
CspCI CAANNNNNGTGG 2 cut(s) 3333, 3368
CviQI GTAC 6 cut(s) 569, 656, 1037, 1233, 1785, 2596
DdeI CTNAG 8 cut(s) 132, 663, 803, 1169, 1280, 1494, 2879, 3339
DraI TTTAAA 1 cut(s) 3288
EaeI YGGCCR 2 cut(s) 228, 2529
Eam1104I CTCTTC 6 cut(s) 270, 996, 1236, 1905, 2133, 2589
EarI CTCTTC 6 cut(s) 270, 996, 1236, 1905, 2133, 2589
EciI GGCGGA 1 cut(s) 2782
Ecl136II GAGCTC 1 cut(s) 2476
Eco130I CCWWGG 3 cut(s) 439, 1539, 3058
Eco24I GRGCYC 4 cut(s) 24, 2478, 2694, 3081
Eco47I GGWCC 3 cut(s) 77, 1657, 3483
Eco53kI GAGCTC 1 cut(s) 2476
Eco57I CTGAAG 4 cut(s) 177, 1353, 1412, 3044
Eco88I CYCGRG 1 cut(s) 2411
EcoICRI GAGCTC 1 cut(s) 2476
EcoO109I RGGNCCY 3 cut(s) 3364, 3483, 3550
EcoRII CCWGG 4 cut(s) 369, 1028, 1307, 3247
EcoT14I CCWWGG 3 cut(s) 439, 1539, 3058
EcoT22I ATGCAT 3 cut(s) 151, 211, 3534
EcoT38I GRGCYC 4 cut(s) 24, 2478, 2694, 3081
ErhI CCWWGG 3 cut(s) 439, 1539, 3058
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 2486, 2518
FaqI GGGAC 3 cut(s) 211, 1152, 3469
FauI CCCGC 3 cut(s) 844, 873, 2867
FauNDI CATATG 2 cut(s) 151, 319
FbaI TGATCA 2 cut(s) 478, 1992
FblI GTMKAC 1 cut(s) 1977
FriOI GRGCYC 4 cut(s) 24, 2478, 2694, 3081
FspAI RTGCGCAY 1 cut(s) 930
FspBI CTAG 7 cut(s) 165, 302, 560, 821, 1121, 1220, 3135
FspI TGCGCA 1 cut(s) 930
GlaI GCGC 3 cut(s) 930, 1323, 2171
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 6 cut(s) 230, 438, 1263, 2531, 3365, 3552
HapII CCGG 3 cut(s) 589, 620, 2864
HgaI GACGC 1 cut(s) 3545
HhaI GCGC 3 cut(s) 931, 1324, 2172
Hin1I GRCGYC 1 cut(s) 1461
Hin6I GCGC 3 cut(s) 929, 1322, 2170
HinP1I GCGC 3 cut(s) 929, 1322, 2170
HincII GTYRAC 2 cut(s) 1217, 3439
HindII GTYRAC 2 cut(s) 1217, 3439
HindIII AAGCTT 2 cut(s) 1301, 3026
HpaII CCGG 3 cut(s) 589, 620, 2864
HphI GGTGA 7 cut(s) 410, 1410, 1469, 1476, 1668, 1794, 1958
Hpy166II GTNNAC 9 cut(s) 1082, 1217, 1235, 1502, 1787, 1978, 2598, 2902, 3439
Hpy188I TCNGA 9 cut(s) 806, 1044, 1431, 1657, 1693, 1698, 1750, 3024, 3340
Hpy8I GTNNAC 9 cut(s) 1082, 1217, 1235, 1502, 1787, 1978, 2598, 2902, 3439
Hpy99I CGWCG 1 cut(s) 2727
HpyAV CCTTC 7 cut(s) 357, 972, 1205, 1765, 1946, 2573, 3473
HpyCH4IV ACGT 4 cut(s) 1141, 1461, 1708, 2725
HpyF3I CTNAG 8 cut(s) 132, 663, 803, 1169, 1280, 1494, 2879, 3339
HpySE526I ACGT 4 cut(s) 1141, 1461, 1708, 2725
Hsp92I GRCGYC 1 cut(s) 1461
HspAI GCGC 3 cut(s) 929, 1322, 2170
KflI GGGWCCC 1 cut(s) 3483
Kpn2I TCCGGA 2 cut(s) 588, 619
Ksp22I TGATCA 2 cut(s) 478, 1992
LguI GCTCTTC 2 cut(s) 996, 2589
LmnI GCTCC 8 cut(s) 727, 1775, 2115, 2473, 2710, 2795, 3076, 3301
LweI GCATC 9 cut(s) 136, 874, 2173, 2376, 2407, 2408, 2994, 3519, 3541
MaeI CTAG 7 cut(s) 165, 302, 560, 821, 1121, 1220, 3135
MaeII ACGT 4 cut(s) 1141, 1461, 1708, 2725
MfeI CAATTG 1 cut(s) 1023
MflI RGATCY 5 cut(s) 695, 832, 1123, 2499, 3019
MhlI GDGCHC 5 cut(s) 24, 2478, 2694, 2954, 3081
MlsI TGGCCA 2 cut(s) 230, 2531
MluNI TGGCCA 2 cut(s) 230, 2531
MlyI GAGTC 3 cut(s) 1475, 1507, 3350
MmeI TCCRAC 3 cut(s) 340, 767, 2879
Mox20I TGGCCA 2 cut(s) 230, 2531
Mph1103I ATGCAT 3 cut(s) 151, 211, 3534
MroI TCCGGA 2 cut(s) 588, 619
MroXI GAANNNNTTC 1 cut(s) 2082
MscI TGGCCA 2 cut(s) 230, 2531
MslI CAYNNNNRTG 5 cut(s) 396, 1199, 1749, 1766, 3207
Msp20I TGGCCA 2 cut(s) 230, 2531
MspI CCGG 3 cut(s) 589, 620, 2864
MspR9I CCNGG 5 cut(s) 371, 1030, 1309, 2864, 3249
MunI CAATTG 1 cut(s) 1023
Mva1269I GAATGC 4 cut(s) 1189, 1320, 2447, 3178
MvaI CCWGG 4 cut(s) 371, 1030, 1309, 3249
MvnI CGCG 1 cut(s) 1324
NciI CCSGG 1 cut(s) 2864
NdeI CATATG 2 cut(s) 151, 319
NheI GCTAGC 1 cut(s) 164
NlaIV GGNNCC 9 cut(s) 368, 1560, 1659, 1888, 2706, 3053, 3078, 3484, 3485
NmeAIII GCCGAG 1 cut(s) 1141
NmuCI GTSAC 6 cut(s) 823, 1072, 1457, 1800, 1958, 2014
NsbI TGCGCA 1 cut(s) 930
NsiI ATGCAT 3 cut(s) 151, 211, 3534
NspI RCATGY 3 cut(s) 929, 3221, 3437
NspV TTCGAA 1 cut(s) 1450
PaeR7I CTCGAG 1 cut(s) 2411
PagI TCATGA 1 cut(s) 544
PciI ACATGT 1 cut(s) 3217
PciSI GCTCTTC 2 cut(s) 996, 2589
PctI GAATGC 4 cut(s) 1189, 1320, 2447, 3178
PdmI GAANNNNTTC 1 cut(s) 2082
PfeI GAWTC 9 cut(s) 33, 625, 1045, 1364, 1664, 2377, 2629, 3335, 3491
PflMI CCANNNNNTGG 1 cut(s) 2993
PleI GAGTC 3 cut(s) 1475, 1506, 3349
PpsI GAGTC 3 cut(s) 1475, 1506, 3349
PpuMI RGGWCCY 1 cut(s) 3483
PscI ACATGT 1 cut(s) 3217
PshBI ATTAAT 1 cut(s) 3377
PsiI TTATAA 5 cut(s) 407, 738, 1178, 1794, 3033
Psp124BI GAGCTC 1 cut(s) 2478
Psp1406I AACGTT 1 cut(s) 1708
Psp5II RGGWCCY 1 cut(s) 3483
Psp6I CCWGG 4 cut(s) 369, 1028, 1307, 3247
PspGI CCWGG 4 cut(s) 369, 1028, 1307, 3247
PspN4I GGNNCC 9 cut(s) 368, 1560, 1659, 1888, 2706, 3053, 3078, 3484, 3485
PspPI GGNCC 5 cut(s) 77, 1657, 3364, 3483, 3550
PspPPI RGGWCCY 1 cut(s) 3483
PstNI CAGNNNCTG 1 cut(s) 2993
PsuI RGATCY 5 cut(s) 695, 832, 1123, 2499, 3019
RsaI GTAC 6 cut(s) 570, 657, 1038, 1234, 1786, 2597
RsaNI GTAC 6 cut(s) 569, 656, 1037, 1233, 1785, 2596
RseI CAYNNNNRTG 5 cut(s) 396, 1199, 1749, 1766, 3207
SacI GAGCTC 1 cut(s) 2478
SapI GCTCTTC 2 cut(s) 996, 2589
Sau96I GGNCC 5 cut(s) 77, 1657, 3364, 3483, 3550
ScaI AGTACT 1 cut(s) 1038
SchI GAGTC 3 cut(s) 1475, 1507, 3350
ScrFI CCNGG 5 cut(s) 371, 1030, 1309, 2864, 3249
SduI GDGCHC 5 cut(s) 24, 2478, 2694, 2954, 3081
SfaNI GCATC 9 cut(s) 136, 874, 2173, 2376, 2407, 2408, 2994, 3519, 3541
SfcI CTRYAG 4 cut(s) 1341, 1939, 2479, 2916
Sfr274I CTCGAG 1 cut(s) 2411
SfuI TTCGAA 1 cut(s) 1450
SinI GGWCC 3 cut(s) 77, 1657, 3483
SlaI CTCGAG 1 cut(s) 2411
SmiMI CAYNNNNRTG 5 cut(s) 396, 1199, 1749, 1766, 3207
SmlI CTYRAG 5 cut(s) 950, 1963, 2411, 2645, 2825
SmoI CTYRAG 5 cut(s) 950, 1963, 2411, 2645, 2825
SpeI ACTAGT 1 cut(s) 1219
SspI AATATT 5 cut(s) 139, 429, 580, 868, 2226
SspMI CTAG 7 cut(s) 165, 302, 560, 821, 1121, 1220, 3135
SstI GAGCTC 1 cut(s) 2478
StyD4I CCNGG 5 cut(s) 369, 1028, 1307, 2862, 3247
StyI CCWWGG 3 cut(s) 439, 1539, 3058
TaiI ACGT 4 cut(s) 1144, 1464, 1711, 2728
TatI WGTACW 3 cut(s) 568, 1036, 1784
TauI GCSGC 5 cut(s) 59, 497, 1168, 1576, 1734
TfiI GAWTC 9 cut(s) 33, 625, 1045, 1364, 1664, 2377, 2629, 3335, 3491
TscAI CASTG 8 cut(s) 22, 79, 999, 1081, 2155, 2443, 2605, 2688
TseFI GTSAC 6 cut(s) 823, 1072, 1457, 1800, 1958, 2014
Tsp45I GTSAC 6 cut(s) 823, 1072, 1457, 1800, 1958, 2014
TspGWI ACGGA 3 cut(s) 2102, 2599, 3512
TspRI CASTG 8 cut(s) 22, 79, 999, 1081, 2155, 2443, 2605, 2688
Van91I CCANNNNNTGG 1 cut(s) 2993
VpaK11BI GGWCC 3 cut(s) 77, 1657, 3483
VspI ATTAAT 1 cut(s) 3377
XapI RAATTY 1 cut(s) 790
XbaI TCTAGA 1 cut(s) 1120
XceI RCATGY 3 cut(s) 929, 3221, 3437
XcmI CCANNNNNNNNNTGG 1 cut(s) 3307
XhoI CTCGAG 1 cut(s) 2411
XmiI GTMKAC 1 cut(s) 1977
XmnI GAANNNNTTC 1 cut(s) 2082
XspI CTAG 7 cut(s) 165, 302, 560, 821, 1121, 1220, 3135
ZraI GACGTC 1 cut(s) 1462
ZrmI AGTACT 1 cut(s) 1038
Zsp2I ATGCAT 3 cut(s) 151, 211, 3534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.