MD01G1121900.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015828
Physical Location & Seq
Forward (+)
23528094 .. 23529700
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1121900.v1.1.491

Sequence Viewer

Length: 759 bp
ATGCCAATCAACAGAATTGCAGTTGGAACGCCGGGAGAGGCAAGCCAACCTGATGCAATTAGGGCTGCGTTGGCTGAGTTTTTTTCCATGCTTATTTTTGTCTTTGCTGGGGAAGGCTCTGGCATGGCTTTCAACAAGCTTACTGACAATGCGTCAACCACACCGTCGGGGCTCGTAGCTGCATCATTAGCCCATGCATTTGCACTTTTTGTGGCGGTCTCAGTGGGTGCAAATATTTCTGGTGGTCATGTAAACCCTGCTGTCACATTTGGTGCATTCATTGGTGGAAACATAACACTCTTGAGGGGCATTTTGTATTGGATTGCACAGTTGTTGGGATCAGTTGTCGCTTGCTTACTGCTCAAGTTTGCCACCGGGGGATGGGAAACGGCTGGATTCTCACTATCGTCTGGCGTATCGGTATGGAATGCTCTAGTGTTTGAGATTGTGATGACCTTCGGCTTGGTCTACACAGTGTATGCCACAGCAGTGGATCCAAAGAAGGGGAATGTAGGGATTGTAGCACCAATTGCAATTGGTTTAATTGTTGGTGCAAACATCTTGGCTGGTGGTGCGTTTGATGGTGCATCTATGAACCCAGCTGTGTCATTTGGGCCTGCTGTGGTCAGTTGGTCATGGACCCACCACTGGGTCTATTGGGCCGGCCCACTAATTGGTGCGGCCATTGCAGCTCTTGTCTATGACAACATCTTCATTGGTAATAGGACCCATGAACCCCTTCCCAACAATGATTTTTAG

Protein Analysis

253

Amino Acids

26.03

Weight (kDa)

5.62

Isoelectric Point (pI)

24.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 14 - 234 7.1e-78 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01470
fragaria_vesca FvH4_7g21520
malus_domestica MD01G1121900.v1.1
prunus_persica Prupe.2G229500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365831
rosa_laevigata RLG00000027388
rosa_multiflora Rmu_sc0004311.1_g000001 Rmu_sc0023137.1_g000003
rosa_roxburghii Rroxscaffold_4G00290830
rosa_rugosa Rorug01G0324900
rosa_samantha Rh1AG333800 Rh1BG294400 Rh1CG311100 Rh1DG326800
rosa_wichuraiana Rw1G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 674
AccI GTMKAC 1 cut(s) 468
AciI CCGC 2 cut(s) 215, 680
AclWI GGATC 3 cut(s) 346, 488, 501
AcoI YGGCCR 1 cut(s) 681
AfiI CCNNNNNNNGG 5 cut(s) 381, 503, 648, 649, 674
AgsI TTSAA 1 cut(s) 133
AhdI GACNNNNNGTC 1 cut(s) 151
AleI CACNNNNGTG 1 cut(s) 488
AluBI AGCT 4 cut(s) 139, 179, 602, 692
AluI AGCT 4 cut(s) 139, 179, 602, 692
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 3 cut(s) 346, 488, 501
AoxI GGCC 4 cut(s) 614, 660, 664, 681
ApeKI GCWGC 3 cut(s) 65, 179, 689
Asp700I GAANNNNTTC 1 cut(s) 738
AspS9I GGNCC 5 cut(s) 614, 639, 660, 665, 726
AsuC2I CCSGG 2 cut(s) 33, 376
AvaII GGWCC 2 cut(s) 639, 726
BamHI GGATCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 52, 166, 701
BccI CCATC 2 cut(s) 375, 575
BceAI ACGGC 1 cut(s) 405
BcnI CCSGG 2 cut(s) 33, 376
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 434
BisI GCNGC 4 cut(s) 66, 180, 681, 690
BlsI GCNGC 4 cut(s) 67, 181, 682, 691
Bme1390I CCNGG 2 cut(s) 33, 376
Bme18I GGWCC 2 cut(s) 639, 726
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgT120I GGNCC 5 cut(s) 614, 639, 660, 665, 726
BmiI GGNNCC 3 cut(s) 495, 641, 728
BmrFI CCNGG 2 cut(s) 33, 376
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 3 cut(s) 43, 191, 596
BmuI ACTGGG 1 cut(s) 658
BpuEI CTTGAG 2 cut(s) 322, 347
BpuMI CCSGG 2 cut(s) 33, 376
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 1 cut(s) 375
Bsc4I CCNNNNNNNGG 5 cut(s) 381, 503, 648, 649, 674
Bse118I RCCGGY 1 cut(s) 662
Bse1I ACTGG 1 cut(s) 653
Bse3DI GCAATG 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 1 cut(s) 386
BseLI CCNNNNNNNGG 5 cut(s) 381, 503, 648, 649, 674
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 2 cut(s) 66, 234
BseNI ACTGG 1 cut(s) 653
BseXI GCAGC 3 cut(s) 52, 166, 701
BseYI CCCAGC 2 cut(s) 107, 598
BshFI GGCC 4 cut(s) 616, 662, 666, 683
BsiSI CCGG 3 cut(s) 32, 375, 663
BslI CCNNNNNNNGG 5 cut(s) 381, 503, 648, 649, 674
BsmAI GTCTC 1 cut(s) 223
BsmI GAATGC 2 cut(s) 275, 433
BsnI GGCC 4 cut(s) 616, 662, 666, 683
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 338, 493
BspACI CCGC 2 cut(s) 215, 680
BspANI GGCC 4 cut(s) 616, 662, 666, 683
BspCNI CTCAG 2 cut(s) 67, 233
BspLI GGNNCC 3 cut(s) 495, 641, 728
BspPI GGATC 3 cut(s) 346, 488, 501
BspTNI GGTCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 684
BsrFI RCCGGY 1 cut(s) 662
BsrI ACTGG 1 cut(s) 653
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 2 cut(s) 338, 493
Bst4CI ACNGT 3 cut(s) 165, 330, 475
BstAPI GCANNNNNTGC 1 cut(s) 530
BstC8I GCNNGC 4 cut(s) 43, 352, 618, 664
BstDEI CTNAG 2 cut(s) 75, 220
BstF5I GGATG 1 cut(s) 386
BstKTI GATC 2 cut(s) 341, 496
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 2 cut(s) 338, 493
BstMWI GCNNNNNNNGC 7 cut(s) 62, 71, 188, 530, 572, 686, 689
BstSCI CCNGG 2 cut(s) 31, 374
BstV1I GCAGC 3 cut(s) 52, 166, 701
BstX2I RGATCY 1 cut(s) 493
BstXI CCANNNNNNTGG 1 cut(s) 490
BstYI RGATCY 1 cut(s) 493
BsuRI GGCC 4 cut(s) 616, 662, 666, 683
BtsCI GGATG 1 cut(s) 386
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 4 cut(s) 228, 480, 495, 646
Cac8I GCNNGC 4 cut(s) 43, 352, 618, 664
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 5 cut(s) 614, 639, 660, 665, 726
CseI GACGC 1 cut(s) 141
CviAII CATG 6 cut(s) 88, 124, 194, 248, 636, 731
DdeI CTNAG 2 cut(s) 75, 220
DpnI GATC 2 cut(s) 340, 495
DpnII GATC 2 cut(s) 338, 493
DriI GACNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 681
Eam1105I GACNNNNNGTC 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 639, 726
EcoO109I RGGNCCY 1 cut(s) 726
EcoT22I ATGCAT 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 174
FaeI CATG 6 cut(s) 91, 127, 197, 251, 639, 734
FatI CATG 6 cut(s) 87, 123, 193, 247, 635, 730
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 4 cut(s) 66, 180, 681, 690
FokI GGATG 1 cut(s) 393
FriOI GRGCYC 1 cut(s) 174
FseI GGCCGGCC 1 cut(s) 666
Fsp4HI GCNGC 4 cut(s) 66, 180, 681, 690
FspBI CTAG 1 cut(s) 434
GluI GCNGC 4 cut(s) 66, 180, 681, 690
GsaI CCCAGC 2 cut(s) 111, 602
HaeIII GGCC 4 cut(s) 616, 662, 666, 683
HapII CCGG 3 cut(s) 32, 375, 663
HgaI GACGC 1 cut(s) 141
Hin1II CATG 6 cut(s) 91, 127, 197, 251, 639, 734
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 1 cut(s) 396
HpaII CCGG 3 cut(s) 32, 375, 663
Hpy166II GTNNAC 3 cut(s) 156, 253, 469
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 3 cut(s) 156, 253, 469
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 4 cut(s) 107, 466, 496, 749
HpyCH4III ACNGT 3 cut(s) 165, 330, 475
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 71, 188, 530, 572, 686, 689
HpyF3I CTNAG 2 cut(s) 75, 220
Hsp92II CATG 6 cut(s) 91, 127, 197, 251, 639, 734
KroI GCCGGC 1 cut(s) 662
KroNI GCCGGC 1 cut(s) 664
Kzo9I GATC 2 cut(s) 338, 493
Lsp1109I GCAGC 3 cut(s) 52, 166, 701
LweI GCATC 3 cut(s) 43, 191, 596
MaeI CTAG 1 cut(s) 434
MaeIII GTNAC 1 cut(s) 262
MalI GATC 2 cut(s) 340, 495
MboI GATC 2 cut(s) 338, 493
MboII GAAGA 1 cut(s) 703
MfeI CAATTG 2 cut(s) 528, 534
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 6 cut(s) 15, 57, 528, 534, 543, 672
MmeI TCCRAC 1 cut(s) 4
MnlI CCTC 2 cut(s) 31, 297
Mph1103I ATGCAT 1 cut(s) 199
MroNI GCCGGC 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 1 cut(s) 542
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 602
MspI CCGG 3 cut(s) 32, 375, 663
MspR9I CCNGG 2 cut(s) 33, 376
MunI CAATTG 2 cut(s) 528, 534
Mva1269I GAATGC 2 cut(s) 275, 433
MwoI GCNNNNNNNGC 7 cut(s) 62, 71, 188, 530, 572, 686, 689
NaeI GCCGGC 1 cut(s) 664
NciI CCSGG 2 cut(s) 33, 376
NdeII GATC 2 cut(s) 338, 493
NgoMIV GCCGGC 1 cut(s) 662
NlaIII CATG 6 cut(s) 91, 127, 197, 251, 639, 734
NlaIV GGNNCC 3 cut(s) 495, 641, 728
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 488
PctI GAATGC 2 cut(s) 275, 433
PdiI GCCGGC 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 1 cut(s) 396
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 4 cut(s) 67, 181, 682, 691
PpuMI RGGWCCY 1 cut(s) 726
Psp5II RGGWCCY 1 cut(s) 726
PspFI CCCAGC 2 cut(s) 107, 598
PspN4I GGNNCC 3 cut(s) 495, 641, 728
PspPI GGNCC 5 cut(s) 614, 639, 660, 665, 726
PspPPI RGGWCCY 1 cut(s) 726
PsuI RGATCY 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 602
RigI GGCCGGCC 1 cut(s) 666
RseI CAYNNNNRTG 1 cut(s) 488
SaqAI TTAA 1 cut(s) 542
SatI GCNGC 4 cut(s) 66, 180, 681, 690
Sau3AI GATC 2 cut(s) 338, 493
Sau96I GGNCC 5 cut(s) 614, 639, 660, 665, 726
ScrFI CCNGG 2 cut(s) 33, 376
SduI GDGCHC 1 cut(s) 174
SetI ASST 6 cut(s) 52, 141, 181, 458, 604, 694
SfaNI GCATC 3 cut(s) 43, 191, 596
SinI GGWCC 2 cut(s) 639, 726
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 301, 362
SmoI CTYRAG 2 cut(s) 301, 362
Sse9I AATT 6 cut(s) 15, 57, 528, 534, 543, 672
SsiI CCGC 2 cut(s) 215, 680
SspI AATATT 1 cut(s) 235
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 2 cut(s) 31, 374
TaaI ACNGT 3 cut(s) 165, 330, 475
TasI AATT 6 cut(s) 15, 57, 528, 534, 543, 672
TauI GCSGC 1 cut(s) 683
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TscAI CASTG 4 cut(s) 228, 480, 495, 653
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 3 cut(s) 65, 179, 689
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 4 cut(s) 268, 608, 703, 747
TspRI CASTG 4 cut(s) 228, 480, 495, 653
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 639, 726
XmiI GTMKAC 1 cut(s) 468
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.