RchiOBHm_Chr1g0365831

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015828
Physical Location & Seq
Forward (+)
56642794 .. 56644087
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59043

Sequence Viewer

Length: 759 bp
ATGGCGATCTCTAGAATTGCAGTTGGGACACCAGGAGAGGCAAGCCACCCTGATGCAATAAGGGCTGCCTTTGCTGAGTTTTTCTCTATGCTTATTTTTGTTTTTGCCGGGGAAGGTTCTGGCATGGCTTTCAGTAAGCTTACTAACAATGGATCAGCCACACCGTCTGGTCTGGTAGCTGCATCATTGTCACATGCGTTTGCGCTATTCGTGGCGGTCTCAGTTGGTGCAAACATTTCTGGAGGCCATGTAAACCCTGCTGTGACATTTGGTGCATTCCTTGGTGGGAACATAACACTTTTGAGGGCCATTCTGTATTGGATTGCGCAGCTTCTGGGATCGGTTGTTGCTTGCTTGTTGCTCAAGTTTGCCACTGGCGGATTGGAAACAGCAGCATTTTCTCTGTCCTCTGGTGTTTCTATATGGAATGCTTTGGTGTTTGAGATCGTGATGACCTTCGGCTTGGTCTACACAGTTTATGCCACAGCAGTGGATCCGAAGAAAGGGAATGTAGGCATTGTAGCACCTATCGCAATTGGTTTGATAGTGGGAGCCAACATCTTGGCTGGTGGTGCTTTTGATGGTGCATCTATGAACCCAGCCGTGTCATTCGGGCCTGCTGTGGTCAGTTGGTCATGGAATCACCACTGGGTCTACTGGGCCGGCCCGTTTATTGGTGCCGCAATTGCTGCTCTTGTCTATGACAACATGTTCATCGGTGATGGAGCTCATGAACCGCTTCCCCACAATGACTTTTAG

Protein Analysis

252

Amino Acids

25.89

Weight (kDa)

5.7

Isoelectric Point (pI)

21.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 14 - 234 6.2e-77 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01470
fragaria_vesca FvH4_7g21520
malus_domestica MD01G1121900.v1.1
prunus_persica Prupe.2G229500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365831
rosa_laevigata RLG00000027388
rosa_multiflora Rmu_sc0004311.1_g000001 Rmu_sc0023137.1_g000003
rosa_roxburghii Rroxscaffold_4G00290830
rosa_rugosa Rorug01G0324900
rosa_samantha Rh1AG333800 Rh1BG294400 Rh1CG311100 Rh1DG326800
rosa_wichuraiana Rw1G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 677
AccI GTMKAC 2 cut(s) 468, 654
AciI CCGC 4 cut(s) 215, 378, 681, 737
AclWI GGATC 4 cut(s) 160, 346, 488, 501
AfiI CCNNNNNNNGG 2 cut(s) 503, 674
AflIII ACRYGT 1 cut(s) 708
AjnI CCWGG 1 cut(s) 31
AleI CACNNNNGTG 1 cut(s) 488
AluBI AGCT 4 cut(s) 139, 179, 331, 728
AluI AGCT 4 cut(s) 139, 179, 331, 728
Alw21I GWGCWC 1 cut(s) 730
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 4 cut(s) 160, 346, 488, 501
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 5 cut(s) 244, 306, 614, 660, 664
ApeKI GCWGC 5 cut(s) 65, 179, 328, 392, 689
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 2 cut(s) 205, 328
AspS9I GGNCC 4 cut(s) 306, 614, 660, 665
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 635, 731
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 1 cut(s) 677
BanII GRGCYC 1 cut(s) 730
Bbv12I GWGCWC 1 cut(s) 730
BbvI GCAGC 5 cut(s) 52, 166, 340, 404, 676
BccI CCATC 2 cut(s) 575, 716
BceAI ACGGC 1 cut(s) 587
BciT130I CCWGG 1 cut(s) 33
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 12
BisI GCNGC 6 cut(s) 66, 180, 329, 393, 681, 690
BlsI GCNGC 6 cut(s) 67, 181, 330, 394, 682, 691
Bme1390I CCNGG 2 cut(s) 33, 109
BmgT120I GGNCC 4 cut(s) 306, 614, 660, 665
BmiI GGNNCC 3 cut(s) 495, 553, 679
BmrFI CCNGG 2 cut(s) 33, 109
BmrI ACTGGG 2 cut(s) 658, 667
BmsI GCATC 3 cut(s) 43, 191, 596
BmuI ACTGGG 2 cut(s) 658, 667
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BpmI CTGGAG 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 109
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 108, 280
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 2 cut(s) 503, 674
Bse118I RCCGGY 1 cut(s) 662
Bse1I ACTGG 3 cut(s) 379, 653, 662
BseBI CCWGG 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 108, 280
BseLI CCNNNNNNNGG 2 cut(s) 503, 674
BseMII CTCAG 2 cut(s) 66, 234
BseNI ACTGG 3 cut(s) 379, 653, 662
BseXI GCAGC 5 cut(s) 52, 166, 340, 404, 676
BseYI CCCAGC 1 cut(s) 598
BshFI GGCC 5 cut(s) 246, 308, 616, 662, 666
BshNI GGYRCC 1 cut(s) 677
BsiHKAI GWGCWC 1 cut(s) 730
BsiSI CCGG 2 cut(s) 108, 663
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 2 cut(s) 503, 674
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 2 cut(s) 275, 433
BsnI GGCC 5 cut(s) 246, 308, 616, 662, 666
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 5 cut(s) 6, 152, 338, 444, 493
BspACI CCGC 4 cut(s) 215, 378, 681, 737
BspANI GGCC 5 cut(s) 246, 308, 616, 662, 666
BspCNI CTCAG 2 cut(s) 67, 233
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 3 cut(s) 495, 553, 679
BspPI GGATC 4 cut(s) 160, 346, 488, 501
BspT107I GGYRCC 1 cut(s) 677
BspTNI GGTCTC 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 662
BsrI ACTGG 3 cut(s) 379, 653, 662
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 2 cut(s) 108, 280
BssMI GATC 5 cut(s) 6, 152, 338, 444, 493
BssT1I CCWWGG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 2 cut(s) 165, 475
BstAPI GCANNNNNTGC 1 cut(s) 689
BstC8I GCNNGC 4 cut(s) 43, 352, 618, 664
BstDEI CTNAG 2 cut(s) 75, 220
BstHHI GCGC 2 cut(s) 205, 328
BstKTI GATC 5 cut(s) 9, 155, 341, 447, 496
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 5 cut(s) 6, 152, 338, 444, 493
BstMWI GCNNNNNNNGC 6 cut(s) 62, 71, 530, 572, 686, 689
BstNI CCWGG 1 cut(s) 33
BstNSI RCATGY 2 cut(s) 197, 712
BstSCI CCNGG 2 cut(s) 31, 107
BstV1I GCAGC 5 cut(s) 52, 166, 340, 404, 676
BstX2I RGATCY 1 cut(s) 493
BstXI CCANNNNNNTGG 2 cut(s) 490, 562
BstYI RGATCY 1 cut(s) 493
BsuRI GGCC 5 cut(s) 246, 308, 616, 662, 666
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 3 cut(s) 372, 495, 646
Cac8I GCNNGC 4 cut(s) 43, 352, 618, 664
CaiI CAGNNNCTG 1 cut(s) 334
CciI TCATGA 1 cut(s) 730
CfoI GCGC 2 cut(s) 205, 328
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 4 cut(s) 306, 614, 660, 665
CviAII CATG 6 cut(s) 124, 194, 248, 636, 709, 731
DdeI CTNAG 2 cut(s) 75, 220
DpnI GATC 5 cut(s) 8, 154, 340, 446, 495
DpnII GATC 5 cut(s) 6, 152, 338, 444, 493
EciI GGCGGA 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 728
Eco130I CCWWGG 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 730
Eco31I GGTCTC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 6 cut(s) 127, 197, 251, 639, 712, 734
FaqI GGGAC 1 cut(s) 40
FatI CATG 6 cut(s) 123, 193, 247, 635, 708, 730
FblI GTMKAC 2 cut(s) 468, 654
Fnu4HI GCNGC 6 cut(s) 66, 180, 329, 393, 681, 690
FriOI GRGCYC 1 cut(s) 730
FseI GGCCGGCC 1 cut(s) 666
Fsp4HI GCNGC 6 cut(s) 66, 180, 329, 393, 681, 690
FspBI CTAG 1 cut(s) 12
FspI TGCGCA 1 cut(s) 327
GlaI GCGC 2 cut(s) 204, 327
GluI GCNGC 6 cut(s) 66, 180, 329, 393, 681, 690
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 5 cut(s) 246, 308, 616, 662, 666
HapII CCGG 2 cut(s) 108, 663
HhaI GCGC 2 cut(s) 205, 328
Hin1II CATG 6 cut(s) 127, 197, 251, 639, 712, 734
Hin6I GCGC 2 cut(s) 203, 326
HinP1I GCGC 2 cut(s) 203, 326
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 1 cut(s) 640
HpaII CCGG 2 cut(s) 108, 663
HphI GGTGA 2 cut(s) 635, 731
Hpy166II GTNNAC 3 cut(s) 253, 469, 655
Hpy188I TCNGA 1 cut(s) 498
Hpy188III TCNNGA 4 cut(s) 12, 240, 448, 731
Hpy8I GTNNAC 3 cut(s) 253, 469, 655
HpyAV CCTTC 2 cut(s) 107, 466
HpyCH4III ACNGT 2 cut(s) 165, 475
HpyCH4V TGCA 6 cut(s) 20, 56, 182, 230, 275, 587
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 71, 530, 572, 686, 689
HpyF3I CTNAG 2 cut(s) 75, 220
Hsp92II CATG 6 cut(s) 127, 197, 251, 639, 712, 734
HspAI GCGC 2 cut(s) 203, 326
KroI GCCGGC 1 cut(s) 662
KroNI GCCGGC 1 cut(s) 664
Kzo9I GATC 5 cut(s) 6, 152, 338, 444, 493
LmnI GCTCC 2 cut(s) 551, 725
Lsp1109I GCAGC 5 cut(s) 52, 166, 340, 404, 676
LweI GCATC 3 cut(s) 43, 191, 596
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 2 cut(s) 189, 262
MalI GATC 5 cut(s) 8, 154, 340, 446, 495
MboI GATC 5 cut(s) 6, 152, 338, 444, 493
MboII GAAGA 1 cut(s) 511
MfeI CAATTG 2 cut(s) 534, 684
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 3 cut(s) 15, 534, 684
MnlI CCTC 4 cut(s) 31, 236, 297, 418
MroNI GCCGGC 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 738
MslI CAYNNNNRTG 2 cut(s) 51, 488
MspI CCGG 2 cut(s) 108, 663
MspR9I CCNGG 2 cut(s) 33, 109
MunI CAATTG 2 cut(s) 534, 684
Mva1269I GAATGC 2 cut(s) 275, 433
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 6 cut(s) 62, 71, 530, 572, 686, 689
NaeI GCCGGC 1 cut(s) 664
NciI CCSGG 1 cut(s) 109
NdeII GATC 5 cut(s) 6, 152, 338, 444, 493
NgoMIV GCCGGC 1 cut(s) 662
NlaIII CATG 6 cut(s) 127, 197, 251, 639, 712, 734
NlaIV GGNNCC 3 cut(s) 495, 553, 679
NmuCI GTSAC 2 cut(s) 189, 262
NsbI TGCGCA 1 cut(s) 327
NspI RCATGY 2 cut(s) 197, 712
OliI CACNNNNGTG 1 cut(s) 488
PagI TCATGA 1 cut(s) 730
PciI ACATGT 1 cut(s) 708
PctI GAATGC 2 cut(s) 275, 433
PdiI GCCGGC 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 1 cut(s) 640
PkrI GCNGC 6 cut(s) 67, 181, 330, 394, 682, 691
PscI ACATGT 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 730
Psp6I CCWGG 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 3 cut(s) 495, 553, 679
PspPI GGNCC 4 cut(s) 306, 614, 660, 665
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 493
RigI GGCCGGCC 1 cut(s) 666
RseI CAYNNNNRTG 2 cut(s) 51, 488
SacI GAGCTC 1 cut(s) 730
SatI GCNGC 6 cut(s) 66, 180, 329, 393, 681, 690
Sau3AI GATC 5 cut(s) 6, 152, 338, 444, 493
Sau96I GGNCC 4 cut(s) 306, 614, 660, 665
ScrFI CCNGG 2 cut(s) 33, 109
SduI GDGCHC 1 cut(s) 730
SetI ASST 7 cut(s) 118, 141, 181, 333, 458, 529, 730
SfaNI GCATC 3 cut(s) 43, 191, 596
SmiMI CAYNNNNRTG 2 cut(s) 51, 488
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 15, 534, 684
SsiI CCGC 4 cut(s) 215, 378, 681, 737
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 730
StyD4I CCNGG 2 cut(s) 31, 107
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 2 cut(s) 165, 475
TasI AATT 3 cut(s) 15, 534, 684
TauI GCSGC 1 cut(s) 683
TfiI GAWTC 1 cut(s) 640
TscAI CASTG 3 cut(s) 379, 495, 653
TseFI GTSAC 2 cut(s) 189, 262
TseI GCWGC 5 cut(s) 65, 179, 328, 392, 689
Tsp45I GTSAC 2 cut(s) 189, 262
TspDTI ATGAA 3 cut(s) 608, 703, 747
TspRI CASTG 3 cut(s) 379, 495, 653
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 2 cut(s) 197, 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XmiI GTMKAC 2 cut(s) 468, 654
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.