MD01G1131000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011910
Physical Location & Seq
Reverse (-)
24163195 .. 24167127
3933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1131000.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGGCAGCTCCAACTCAAGCCCTAACTCAGCGTCAACTCCTCAAAACCCTAACCAACCTCTTGACCTCCACAAAAATCCTAAACCCAGAACCCCTAAAACCCTACATTCCTCACCTGACCCAACCCCTCCTCCTCTCCATCATCTCCTCCAAAGCCCTAGCCTCTCAACCCACCGCCCTCCTCTTCTTCTTCCGGTGGGTCCAGGATCACACGCCCTCCCTAACCCAATCCCCACAGACCCTCCTCGCCCTCCTCCCCTCCCTCTTCACCCACCACAAGTTCTCCGACGCCAAAGCCCTCCTCGTCCAATTCATCGCCGCCGATCGCAAGAACGATCTCCACCGCCTCATTCTTCACCCGGACCCCACCGTGCCGAAGCCCTCGAAGGCCCTTCTGGACACTTCCATTGGGGCGTATGTGGAATCCGGGAAACCCCACCTTGCAGCCCAAGTGTTTGATAAAATGAAGCGGCTTCGGCTTCAGCCCAATTTGCTCACCTGCAATACTCTGATTAATGGTCTGGTAAGGTACCGGTCATCGCAATCGATTTCATTGTCTAGAGGTGTGTTTAAGCATGCTGTTGAGTTAGGGGTTAAAGTGAATACAAATACTTTTAACATTTTGATATGTGGGTATTGCTTGGAGCATAAGTTTAGGGATGCTGTAGAGTTGTTGAGTAGGATGAGTGAATTCAGATGCATGCCCGATAATGTGAGCTATAATACGATATTGAACTGGCTGTGTAAGAAGGGGCAGTTGAGTGAGGCCCGGGACTTGTTGTTGGACATGAAGAATCGAGGGTTGTTTCCGAATAGGAACACGTTTAACATTTTGGTTTGTGGGTATTGTAAGATGGGATGGTTGAAGGAGGCAATGCAGGTGATTGAGTTGATGACACAGAACAGTTTGTTGCCGGATATTTGGACGTACAATGTCTTGATTAAGGGGTTGTGTAGGGAGGGTAGGATTGAGGAGGCTCTGAGGCTTCGGGTTGAGATGGAAAATTTGAGGTTGATGCCTGATGTTGTCACGTATAACACATTGATTGATGGGTATTTTGAGTGGAGCAGCGATTCAGAGGCCTTTAAGTTGGTTGAGGATATGCATGAAAAGGGAGTGAAAGCAAATGCAGTTACTTATAATATAATAGTAAAGTGGTTCTGTAAAGAAGGGAAGCTGGATGAAGCCAGTGATACTATAAGGAAGATGGAGGAAGATGGGTTTGCTCCTGATTGTGTCACTTATAATACTTTGATTAACGAGTATTGCAAGGCAGGAAAAATGGCAGAAGCATTTAAAATGATGAATAAGATGGGTACGAAAGGTTTGAAGATGGACATCTTTACTCTCAATACTCTTCTTTACACTCTCTGCAGTGAGAAGAAGCTGGACGAGGCATTTGAGTTGCTTCGTACTGCAACTAAGCGGGGTTATATTCTTGATGAGGTAAGCTATGGAACCTTGATCACGGGATGCTTTAAGAACGAAAAGGCCAACAAGGGTTTCAAGCTTTGGGATGAGATGAAGGAGAAGCAGGTTGTTCCCAGCATTGTCACCTATAACACTATAATTGGAGGCCTCTGCCAGTCTGGAAAAACTGATCAAGCATTTGACAAGTTGAATGAGCTTCTAGAGAGGGGTCTAGTCCCGGATGGAATTACATACAACACAATAATTCATGGTTACTGCCGTGAGGGGAATGTTGAGAAAGCATTTCAGTTCCACAACAAAATGGTTGAGAAATCATTCAAGCCAGATGTCTATACATGTAATATTCTTCTTTGTGGGCTATGTAGAGAGGGTATGTTAGAAAAAGCTCTTAAGCTTTTTAACACATGGATTTCGAAAGGGAAAGACATTGATGCAGTTACTTACAACACATTGATATCAAGCCTTTGCAAAGAAGGAAGGTTTGATGATGTTTTTGCTCTTTTTTCGGAAATGGAAGAAAAGAAATTAGCACCAGACCAGTATACATACACTGCCATTCTGGGTGCACTTACCGATACTGGTAGGATTAAGGAAGCAGAAGAATTTATTTTGAAAATGATTGATATGGGAAAGTTACCTGATTGGCCCCCAGAATTGGAGAAGGTTAAAAATGGAGTGGCCGAATCTTCAGTTGAATATGATTCAAGCACAGGAGCCTATTCAGAGAAAATCATTGAGTGTTGCTTTCAAGGTAAATATAAGGATGCAATGCACATTTTGGAAGAGTCAATGCAGAAAGGCATGACGTTAAATAAAGATGTTTACATTAATTTGATGAATGGGCTGATTAAGAGGCGAAAAAGCATATCAAAAGCTGTTAAGCTGTAG

Protein Analysis

773

Amino Acids

87.71

Weight (kDa)

8.44

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 136 - 176 2.7e-08 PPR repeat family
PPR_3 PF13812 189 - 246 4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 197 - 229 1.2e-07 PPR repeat
PPR_2 PF13041 201 - 242 9.7e-09 PPR repeat family
PPR_1 PF12854 233 - 264 9.2e-08 PPR repeat
PPR_2 PF13041 235 - 284 1.4e-17 PPR repeat family
PPR PF01535 238 - 268 7.7e-07 PPR repeat
PPR_3 PF13812 259 - 315 1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 266 - 298 3.2e-10 PPR repeat
PPR_2 PF13041 274 - 319 1.1e-12 PPR repeat family
PPR_1 PF12854 303 - 334 8.4e-12 PPR repeat
PPR_2 PF13041 305 - 352 4.3e-16 PPR repeat family
PPR PF01535 309 - 335 3.6e-07 PPR repeat
PPR_3 PF13812 331 - 388 9.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 338 - 368 2.3e-06 PPR repeat
PPR_2 PF13041 340 - 389 2.5e-15 PPR repeat family
PPR_long PF17177 359 - 454 1e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 363 - 422 9.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 371 - 404 8.9e-10 PPR repeat
PPR_2 PF13041 376 - 420 7.3e-14 PPR repeat family
PPR PF01535 378 - 408 1.9e-06 PPR repeat
TPR_24 PF23276 392 - 506 7.8e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 401 - 456 5.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 407 - 438 5.7e-14 PPR repeat
PPR_2 PF13041 410 - 458 3.8e-15 PPR repeat family
PPR PF01535 413 - 443 5.6e-08 PPR repeat
PPR_1 PF12854 441 - 471 6.3e-07 PPR repeat
PPR_2 PF13041 446 - 494 5.4e-09 PPR repeat family
PPR_long PF17177 458 - 594 6.1e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 466 - 577 3.5e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 469 - 525 7.4e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 481 - 520 5.9e-10 PPR repeat family
PPR_1 PF12854 512 - 542 2.8e-09 PPR repeat
PPR_2 PF13041 515 - 559 2e-13 PPR repeat family
PPR PF01535 518 - 548 8e-06 PPR repeat
PPR_3 PF13812 541 - 594 1.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 546 - 579 2.2e-14 PPR repeat
PPR_2 PF13041 550 - 599 1.2e-19 PPR repeat family
PPR PF01535 553 - 582 4.7e-09 PPR repeat
PPR_1 PF12854 582 - 611 9.3e-09 PPR repeat
PPR PF01535 588 - 617 8.9e-06 PPR repeat
PPR_2 PF13041 589 - 625 1.2e-06 PPR repeat family
PPR_3 PF13812 616 - 667 1.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 618 - 649 3.8e-11 PPR repeat
PPR_2 PF13041 621 - 667 1.3e-16 PPR repeat family
PPR PF01535 623 - 652 3.5e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16880
fragaria_vesca FvH4_7g20132
malus_domestica MD01G1131000.v1.1 MD07G1179000.v1.1
prunus_persica Prupe.2G218100_v2.0.a1 Prupe.2G218100_v2.0.a1
pyrus_communis pycom01g13980 pycom07g17270
rosa_chinensis RchiOBHm_Chr1g0364091
rosa_laevigata RLG00000027517
rosa_multiflora Rmu_sc0000071.1_g000021 Rmu_sc0011704.1_g000001
rosa_roxburghii Rroxscaffold_4G00292650
rosa_rugosa Rorug01G0313500
rosa_samantha Rh1AG320900 Rh1BG284100 Rh1CG300500 Rh1DG315500
rosa_wichuraiana Rw1G028450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1140, 1245
AarI CACCTGC 2 cut(s) 506, 868
Acc36I ACCTGC 3 cut(s) 506, 868, 1525
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AccI GTMKAC 1 cut(s) 1973
AciI CCGC 5 cut(s) 174, 318, 343, 469, 1426
AclWI GGATC 1 cut(s) 213
AcoI YGGCCR 1 cut(s) 2109
AcsI RAATTY 3 cut(s) 689, 1003, 2033
AcuI CTGAAG 2 cut(s) 464, 2103
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 4 cut(s) 530, 929, 1318, 1414
AfiI CCNNNNNNNGG 1 cut(s) 2086
AflII CTTAAG 1 cut(s) 1820
AflIII ACRYGT 2 cut(s) 819, 1766
AgeI ACCGGT 1 cut(s) 531
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 1487, 1519
Alw21I GWGCWC 1 cut(s) 1999
Alw44I GTGCAC 1 cut(s) 1995
AlwI GGATC 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 768
AoxI GGCC 7 cut(s) 387, 765, 1080, 1491, 1576, 2075, 2109
ApaLI GTGCAC 1 cut(s) 1995
ApeKI GCWGC 3 cut(s) 5, 443, 1068
ApoI RAATTY 3 cut(s) 689, 1003, 2033
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
AseI ATTAAT 2 cut(s) 513, 2259
AsiGI ACCGGT 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 1745
Asp718I GGTACC 1 cut(s) 528
AspS9I GGNCC 5 cut(s) 199, 361, 388, 766, 2076
AsuC2I CCSGG 5 cut(s) 359, 427, 769, 770, 1649
AsuHPI GGTGA 6 cut(s) 104, 259, 347, 487, 892, 1546
AsuII TTCGAA 1 cut(s) 1844
AvaI CYCGRG 1 cut(s) 768
AvaII GGWCC 2 cut(s) 199, 361
BaeGI GKGCMC 1 cut(s) 1999
BanI GGYRCC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 1999
BbvI GCAGC 3 cut(s) 17, 455, 1080
BceAI ACGGC 1 cut(s) 1674
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 2 cut(s) 1464, 1600
BcnI CCSGG 5 cut(s) 359, 427, 769, 770, 1649
BfaI CTAG 4 cut(s) 158, 558, 1631, 1643
BfmI CTRYAG 3 cut(s) 663, 1372, 2315
BfrI CTTAAG 1 cut(s) 1820
BfuAI ACCTGC 3 cut(s) 506, 868, 1525
BisI GCNGC 5 cut(s) 6, 318, 444, 470, 1069
BlsI GCNGC 5 cut(s) 7, 319, 445, 471, 1070
Bme1390I CCNGG 6 cut(s) 203, 359, 427, 769, 770, 1649
Bme18I GGWCC 2 cut(s) 199, 361
BmeT110I CYCGRG 1 cut(s) 768
BmgT120I GGNCC 5 cut(s) 199, 361, 388, 766, 2076
BmiI GGNNCC 6 cut(s) 200, 363, 530, 1459, 2078, 2146
BmrFI CCNGG 6 cut(s) 203, 359, 427, 769, 770, 1649
BmsI GCATC 6 cut(s) 649, 686, 1005, 1463, 1852, 2185
Bpu14I TTCGAA 1 cut(s) 1844
BpuMI CCSGG 5 cut(s) 359, 427, 769, 770, 1649
Bsa29I ATCGAT 1 cut(s) 545
BsaAI YACGTR 1 cut(s) 1032
BsaBI GATNNNNATC 1 cut(s) 1337
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 768
BsaWI WCCGGW 2 cut(s) 192, 531
Bsc4I CCNNNNNNNGG 1 cut(s) 2086
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 5 cut(s) 740, 1188, 1585, 1969, 2014
Bse3DI GCAATG 2 cut(s) 879, 2205
Bse8I GATNNNNATC 1 cut(s) 1337
BseBI CCWGG 1 cut(s) 203
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 1 cut(s) 768
BseGI GGATG 8 cut(s) 664, 687, 863, 1186, 1478, 1522, 1657, 2200
BseJI GATNNNNATC 1 cut(s) 1337
BseLI CCNNNNNNNGG 1 cut(s) 2086
BseMI GCAATG 2 cut(s) 879, 2205
BseMII CTCAG 2 cut(s) 41, 971
BseNI ACTGG 5 cut(s) 740, 1188, 1585, 1969, 2014
BseRI GAGGAG 9 cut(s) 29, 119, 122, 136, 170, 233, 242, 290, 986
BseSI GKGCMC 1 cut(s) 1999
BseXI GCAGC 3 cut(s) 17, 455, 1080
BseYI CCCAGC 1 cut(s) 1544
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 7 cut(s) 389, 767, 1082, 1493, 1578, 2077, 2111
BshNI GGYRCC 1 cut(s) 528
BshTI ACCGGT 1 cut(s) 531
BshVI ATCGAT 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 1999
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 7 cut(s) 193, 359, 426, 532, 769, 914, 1649
BslFI GGGAC 2 cut(s) 785, 1631
BslI CCNNNNNNNGG 1 cut(s) 2086
BsmFI GGGAC 2 cut(s) 785, 1631
BsnI GGCC 7 cut(s) 389, 767, 1082, 1493, 1578, 2077, 2111
BsoBI CYCGRG 1 cut(s) 768
Bsp119I TTCGAA 1 cut(s) 1844
Bsp1286I GDGCHC 1 cut(s) 1999
Bsp143I GATC 5 cut(s) 205, 322, 334, 1464, 1600
BspACI CCGC 5 cut(s) 174, 318, 343, 469, 1426
BspANI GGCC 7 cut(s) 389, 767, 1082, 1493, 1578, 2077, 2111
BspCNI CTCAG 2 cut(s) 40, 972
BspDI ATCGAT 1 cut(s) 545
BspLI GGNNCC 6 cut(s) 200, 363, 530, 1459, 2078, 2146
BspMAI CTGCAG 1 cut(s) 1376
BspMI ACCTGC 3 cut(s) 506, 868, 1525
BspPI GGATC 1 cut(s) 213
BspT104I TTCGAA 1 cut(s) 1844
BspT107I GGYRCC 1 cut(s) 528
BspTI CTTAAG 1 cut(s) 1820
BsrDI GCAATG 2 cut(s) 879, 2205
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 5 cut(s) 740, 1188, 1585, 1969, 2014
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 1 cut(s) 768
BssMI GATC 5 cut(s) 205, 322, 334, 1464, 1600
BssNAI GTATAC 1 cut(s) 1974
BssNI GRCGYC 1 cut(s) 288
Bst1107I GTATAC 1 cut(s) 1974
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 370, 905
Bst6I CTCTTC 4 cut(s) 188, 269, 1362, 2208
BstACI GRCGYC 1 cut(s) 288
BstAFI CTTAAG 1 cut(s) 1820
BstBAI YACGTR 1 cut(s) 1032
BstBI TTCGAA 1 cut(s) 1844
BstC8I GCNNGC 2 cut(s) 576, 701
BstDEI CTNAG 3 cut(s) 27, 980, 1422
BstF5I GGATG 8 cut(s) 664, 687, 863, 1186, 1478, 1522, 1657, 2200
BstKTI GATC 5 cut(s) 208, 325, 337, 1467, 1603
BstMBI GATC 5 cut(s) 205, 322, 334, 1464, 1600
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 2 cut(s) 475, 490
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 3 cut(s) 578, 703, 1770
BstSCI CCNGG 6 cut(s) 201, 357, 425, 767, 768, 1647
BstSFI CTRYAG 3 cut(s) 663, 1372, 2315
BstSLI GKGCMC 1 cut(s) 1999
BstV1I GCAGC 3 cut(s) 17, 455, 1080
BstZ17I GTATAC 1 cut(s) 1974
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 7 cut(s) 389, 767, 1082, 1493, 1578, 2077, 2111
BsuTUI ATCGAT 1 cut(s) 545
BtgZI GCGATG 2 cut(s) 298, 522
BtsCI GGATG 8 cut(s) 664, 687, 863, 1186, 1478, 1522, 1657, 2200
BtsI GCAGTG 2 cut(s) 1381, 1980
BtsIMutI CAGTG 3 cut(s) 1195, 1381, 1980
BveI ACCTGC 3 cut(s) 506, 868, 1525
Cac8I GCNNGC 2 cut(s) 576, 701
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 5 cut(s) 199, 361, 388, 766, 2076
Cfr9I CCCGGG 1 cut(s) 768
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 2 cut(s) 20, 296
Csp6I GTAC 4 cut(s) 529, 928, 1317, 1413
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 8 cut(s) 575, 700, 787, 1106, 1679, 1767, 1836, 2233
CviQI GTAC 4 cut(s) 529, 928, 1317, 1413
DdeI CTNAG 3 cut(s) 27, 980, 1422
DpnI GATC 5 cut(s) 207, 324, 336, 1466, 1602
DpnII GATC 5 cut(s) 205, 322, 334, 1464, 1600
DraI TTTAAA 1 cut(s) 1297
EaeI YGGCCR 1 cut(s) 2109
Eam1104I CTCTTC 4 cut(s) 188, 269, 1362, 2208
EarI CTCTTC 4 cut(s) 188, 269, 1362, 2208
Eco147I AGGCCT 2 cut(s) 1082, 1578
Eco32I GATATC 1 cut(s) 1887
Eco47I GGWCC 2 cut(s) 199, 361
Eco57I CTGAAG 2 cut(s) 464, 2103
Eco88I CYCGRG 1 cut(s) 768
EcoO109I RGGNCCY 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 689
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 1887
EcoT22I ATGCAT 2 cut(s) 701, 1107
FaeI CATG 8 cut(s) 578, 703, 790, 1109, 1682, 1770, 1839, 2236
FaqI GGGAC 2 cut(s) 785, 1631
FatI CATG 8 cut(s) 574, 699, 786, 1105, 1678, 1766, 1835, 2232
FauI CCCGC 1 cut(s) 1419
FbaI TGATCA 2 cut(s) 1464, 1600
FblI GTMKAC 1 cut(s) 1973
Fnu4HI GCNGC 5 cut(s) 6, 318, 444, 470, 1069
FokI GGATG 8 cut(s) 671, 694, 870, 1193, 1485, 1529, 1664, 2207
Fsp4HI GCNGC 5 cut(s) 6, 318, 444, 470, 1069
FspBI CTAG 4 cut(s) 158, 558, 1631, 1643
GluI GCNGC 5 cut(s) 6, 318, 444, 470, 1069
GsaI CCCAGC 1 cut(s) 1548
HaeIII GGCC 7 cut(s) 389, 767, 1082, 1493, 1578, 2077, 2111
HapII CCGG 7 cut(s) 193, 359, 426, 532, 769, 914, 1649
HgaI GACGC 2 cut(s) 20, 296
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 8 cut(s) 578, 703, 790, 1109, 1682, 1770, 1839, 2236
HincII GTYRAC 1 cut(s) 35
HindII GTYRAC 1 cut(s) 35
HindIII AAGCTT 2 cut(s) 1508, 1823
HinfI GANTC 6 cut(s) 422, 793, 1073, 2114, 2132, 2216
HpaII CCGG 7 cut(s) 193, 359, 426, 532, 769, 914, 1649
HphI GGTGA 6 cut(s) 104, 259, 347, 487, 892, 1546
Hpy166II GTNNAC 4 cut(s) 35, 1974, 1997, 2254
Hpy188I TCNGA 8 cut(s) 286, 510, 695, 810, 981, 1078, 1939, 2155
Hpy188III TCNNGA 8 cut(s) 61, 395, 558, 937, 1229, 1439, 1590, 1631
Hpy8I GTNNAC 4 cut(s) 35, 1974, 1997, 2254
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 9 cut(s) 379, 401, 742, 859, 1163, 1519, 1898, 1902, 2086
HpyCH4III ACNGT 2 cut(s) 370, 905
HpyCH4IV ACGT 4 cut(s) 821, 926, 1031, 2237
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 490
HpyF3I CTNAG 3 cut(s) 27, 980, 1422
HpySE526I ACGT 4 cut(s) 821, 926, 1031, 2237
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 8 cut(s) 578, 703, 790, 1109, 1682, 1770, 1839, 2236
KpnI GGTACC 1 cut(s) 532
Ksp22I TGATCA 2 cut(s) 1464, 1600
Kzo9I GATC 5 cut(s) 205, 322, 334, 1464, 1600
LmnI GCTCC 5 cut(s) 13, 643, 1065, 1231, 2144
Lsp1109I GCAGC 3 cut(s) 17, 455, 1080
LweI GCATC 6 cut(s) 649, 686, 1005, 1463, 1852, 2185
MaeI CTAG 4 cut(s) 158, 558, 1631, 1643
MaeII ACGT 4 cut(s) 821, 926, 1031, 2237
MaeIII GTNAC 7 cut(s) 1027, 1132, 1237, 1552, 1682, 1867, 2064
MalI GATC 5 cut(s) 207, 324, 336, 1466, 1602
MboI GATC 5 cut(s) 205, 322, 334, 1464, 1600
MhlI GDGCHC 1 cut(s) 1999
MlyI GAGTC 1 cut(s) 2225
MmeI TCCRAC 3 cut(s) 35, 309, 762
Mph1103I ATGCAT 2 cut(s) 701, 1107
MroXI GAANNNNTTC 1 cut(s) 1745
MspCI CTTAAG 1 cut(s) 1820
MspI CCGG 7 cut(s) 193, 359, 426, 532, 769, 914, 1649
MspR9I CCNGG 6 cut(s) 203, 359, 427, 769, 770, 1649
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 475, 490
NciI CCSGG 5 cut(s) 359, 427, 769, 770, 1649
NdeII GATC 5 cut(s) 205, 322, 334, 1464, 1600
NlaIII CATG 8 cut(s) 578, 703, 790, 1109, 1682, 1770, 1839, 2236
NlaIV GGNNCC 6 cut(s) 200, 363, 530, 1459, 2078, 2146
NmuCI GTSAC 3 cut(s) 1027, 1237, 1552
NsiI ATGCAT 2 cut(s) 701, 1107
NspI RCATGY 3 cut(s) 578, 703, 1770
NspV TTCGAA 1 cut(s) 1844
PaeI GCATGC 2 cut(s) 578, 703
PaqCI CACCTGC 2 cut(s) 506, 868
PceI AGGCCT 2 cut(s) 1082, 1578
PciI ACATGT 1 cut(s) 1766
PdmI GAANNNNTTC 1 cut(s) 1745
PfeI GAWTC 5 cut(s) 422, 793, 1073, 2114, 2132
PfoI TCCNGGA 3 cut(s) 201, 425, 1647
PinAI ACCGGT 1 cut(s) 531
PkrI GCNGC 5 cut(s) 7, 319, 445, 471, 1070
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 1 cut(s) 2224
PpsI GAGTC 1 cut(s) 2224
Ppu21I YACGTR 1 cut(s) 1032
PscI ACATGT 1 cut(s) 1766
PshBI ATTAAT 2 cut(s) 513, 2259
PsiI TTATAA 2 cut(s) 1140, 1245
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 1544
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 6 cut(s) 200, 363, 530, 1459, 2078, 2146
PspPI GGNCC 5 cut(s) 199, 361, 388, 766, 2076
PstI CTGCAG 1 cut(s) 1376
PvuI CGATCG 1 cut(s) 325
RsaI GTAC 4 cut(s) 530, 929, 1318, 1414
RsaNI GTAC 4 cut(s) 529, 928, 1317, 1413
SatI GCNGC 5 cut(s) 6, 318, 444, 470, 1069
Sau3AI GATC 5 cut(s) 205, 322, 334, 1464, 1600
Sau96I GGNCC 5 cut(s) 199, 361, 388, 766, 2076
SchI GAGTC 1 cut(s) 2225
ScrFI CCNGG 6 cut(s) 203, 359, 427, 769, 770, 1649
SduI GDGCHC 1 cut(s) 1999
SfaNI GCATC 6 cut(s) 649, 686, 1005, 1463, 1852, 2185
SfcI CTRYAG 3 cut(s) 663, 1372, 2315
SfuI TTCGAA 1 cut(s) 1844
SinI GGWCC 2 cut(s) 199, 361
SmaI CCCGGG 1 cut(s) 770
SmlI CTYRAG 2 cut(s) 15, 1820
SmoI CTYRAG 2 cut(s) 15, 1820
SphI GCATGC 2 cut(s) 578, 703
SseBI AGGCCT 2 cut(s) 1082, 1578
SsiI CCGC 5 cut(s) 174, 318, 343, 469, 1426
SspI AATATT 1 cut(s) 1774
SspMI CTAG 4 cut(s) 158, 558, 1631, 1643
StuI AGGCCT 2 cut(s) 1082, 1578
StyD4I CCNGG 6 cut(s) 201, 357, 425, 767, 768, 1647
TaaI ACNGT 2 cut(s) 370, 905
TaiI ACGT 4 cut(s) 824, 929, 1034, 2240
TaqI TCGA 4 cut(s) 383, 545, 796, 1844
TauI GCSGC 2 cut(s) 320, 472
TfiI GAWTC 5 cut(s) 422, 793, 1073, 2114, 2132
TscAI CASTG 3 cut(s) 1195, 1381, 1987
TseFI GTSAC 3 cut(s) 1027, 1237, 1552
TseI GCWGC 3 cut(s) 5, 443, 1068
Tsp45I GTSAC 3 cut(s) 1027, 1237, 1552
TspMI CCCGGG 1 cut(s) 768
TspRI CASTG 3 cut(s) 1195, 1381, 1987
Vha464I CTTAAG 1 cut(s) 1820
VneI GTGCAC 1 cut(s) 1995
VpaK11BI GGWCC 2 cut(s) 199, 361
VspI ATTAAT 2 cut(s) 513, 2259
XapI RAATTY 3 cut(s) 689, 1003, 2033
XbaI TCTAGA 2 cut(s) 557, 1630
XceI RCATGY 3 cut(s) 578, 703, 1770
XmaI CCCGGG 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 1973
XmnI GAANNNNTTC 1 cut(s) 1745
XspI CTAG 4 cut(s) 158, 558, 1631, 1643
Zsp2I ATGCAT 2 cut(s) 701, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.