pycom07g17270

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011910
Physical Location & Seq
Reverse (-)
19787750 .. 19789770
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17270.1

Sequence Viewer

Length: 1959 bp
ATGCCACTTTATTGCTCGTCTTCTTCAACCTGCTGCAACTCACACTCGCAAGTCACGGTTGCCTCCATGGCAGCTCCACCTCAAACCTTAACCCAACCCCAACTCCTCAAAACCCTAACCAACCTCTTGACCTCCACAAAAACCCTAAACCCAGAAACCCTAAAACCCCACATTCCGCATCTGACCCAACCCGTCCTCCTTTCCATCATCTCCTCCAAAACCCTAGCCTCCCAGCCCACCACCCTCCTCTTCTTCTTCCGGTGGGCCCAGGCCCACACGCCCTCCCTAACCCAATCCCCACAAACCCTCCTCGCCCTCCTCCCCTCCCTCTTCACCCACCACAAGTTCTCCGATGCCAAAGCCCTCCTCGTCCAATTCATCGCCGCCGATCGCCAGAACGATCTCCACCGCCTTATTCTACACCCGGACCCCACCGTCCCGAAGCCCTCGAAGGCCCTTCTGGACACTTCCATTGGGGCATATGTGCTATCCGGGAAACCCCACCTTGCAGCCCAAGTGTTTGATAAAATGAAGCGGCTTCGGCTTCAGCCCAATTTACTCACCTGCAATACTCTGATTAATGGTCTGGTAAGGTACCGGTCCTCGCATTCGATTTCATTGTCTAGAGGTGTGTTTAAGCATGCTGTTGAGTTAGGGGTTAAAGTGAATACGAATACTTTTAACATTTTGATATATGGGTATTGCTTGGAACATAAGTTTAGGGATGCTGTAGAGTTGTTGAGTAGGATGAGTGAATTCAGATGCGTGCCCGATAATGTGAGCTATAATACGATATTGGACTGGCTGTGTAAGAAGGGGCAGTTGAATGAGGCCCGGGACTTGTTGTTGGACATGAAGAATCGAGGTTTGTTTCCGAATAGGAACACGTATAACATTTTGGTTTGTGGGTATTGTAAGATGGGGTGGTTGAAGGAGGCAATGCAGGTGATTGAATCAATGACACGGAACAGTTTGCTGCCGGATATTTGGACGTACAATGTCTTGATTAAGGGGTTGTGTAGGGAGGGTAGGATTGAGGAGGCTCTGAGACTTCGGGTTGAGATGGAAGATTTGAAGTTGATGCCTGATGTTGTCACGTATAACACATTGATTGATGGATATTTTGAGTGGAGGAGCGATTCAGAGGCCTTTAAGTTGATTGAGGAGATGCGTGAAAAGGGAGTGAAAACAAATGCAGTTACTCACAATATAATAGTAAAGTGGTTCTGTAAAGAAGGGAAGCTGGATGAAGCCAGTGATACTTTAAGGAAGATGGAGGAAGATGGGTTTACTCCTGATTGTGTCACTTATAATACTTTGATTAGTGAGTATTGCAAGGCAGGGAAAATGGCAGAAGCATTTAAAATGATGAATGAGATGGGTATGAAAGGTTTGAAGATGGACATCTTTACTCTCAATACTCTTCTTCACACTCTTTGCAGTGACAAGAAGCTCGATGAAGCATTTGAGTTGTTTCATACTTCAACTAAGCGGGGTTATATTCTTGATGAGGTAAGCTATGGAACCTTGATCATGGGATGCTTTAAGAACGAAAAGGCCAACAAGGGTTTTGAGCTTTGGGATGAGATGAAGGAGAAGCAGGTTGTTCCCAGCATTGCCACCTATAACACTGTAATTGGAGGGCTCTGCCAATCTGGAAAAACTGATCGATCATTCGACAAGTTGAATGAGCTTCTAGAGAGGGGTCTAGTCCCTGATGGAACTACATACAACACGATAATTCATGGTTACTGCCATGAGGGGAATGTTGAGAAAGCGTTTCAGTTCCACAACAAAATGGTTGAGAAATCATTCAAGCCAGATGTCTATACATGTAATACTCTTCTTTGTGGGCTATGTAGAGAGGGTATGTTAGAAAAAGCTCTGAAGCTTTTTAACACATGGATTTCGAAAGGGAAAGACATTGATGCTGTTACTTACAACACGCTAAGGAACTGA

Protein Analysis

653

Amino Acids

74.04

Weight (kDa)

8.51

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 158 - 198 2.5e-07 PPR repeat family
PPR_3 PF13812 159 - 194 9.8e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 211 - 268 8.5e-08 Pentatricopeptide repeat domain
PPR_long PF17177 211 - 344 4.8e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 219 - 251 2.4e-07 PPR repeat
PPR_2 PF13041 223 - 265 1.4e-08 PPR repeat family
PPR_1 PF12854 255 - 286 2.6e-08 PPR repeat
PPR_2 PF13041 257 - 306 1.5e-18 PPR repeat family
PPR PF01535 260 - 290 1e-06 PPR repeat
PPR_3 PF13812 281 - 337 5.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 288 - 321 2.9e-11 PPR repeat
PPR PF01535 296 - 323 1.3e-06 PPR repeat
PPR_2 PF13041 296 - 341 4.2e-14 PPR repeat family
PPR_1 PF12854 325 - 356 6.9e-12 PPR repeat
PPR_2 PF13041 327 - 374 2.3e-16 PPR repeat family
PPR PF01535 331 - 358 2.4e-07 PPR repeat
PPR_3 PF13812 353 - 410 3.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 360 - 391 4.7e-07 PPR repeat
PPR_2 PF13041 362 - 411 4.4e-15 PPR repeat family
PPR PF01535 365 - 395 8.5e-07 PPR repeat
PPR_long PF17177 381 - 477 1.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 393 - 426 9.5e-09 PPR repeat
PPR_2 PF13041 398 - 442 6.1e-12 PPR repeat family
TPR_24 PF23276 413 - 528 9.3e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 428 - 460 2.2e-14 PPR repeat
PPR_2 PF13041 432 - 480 2.5e-16 PPR repeat family
PPR PF01535 435 - 465 4.2e-08 PPR repeat
PPR_1 PF12854 463 - 493 6.1e-08 PPR repeat
PPR_2 PF13041 468 - 514 1.1e-08 PPR repeat family
PPR_long PF17177 480 - 634 7.4e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 492 - 547 1.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 503 - 551 6.6e-14 PPR repeat family
PPR PF01535 505 - 534 1e-05 PPR repeat
PPR_1 PF12854 534 - 564 3.8e-06 PPR repeat
PPR_2 PF13041 537 - 586 1.5e-13 PPR repeat family
PPR_1 PF12854 568 - 601 3.9e-12 PPR repeat
PPR_2 PF13041 572 - 621 1.4e-18 PPR repeat family
PPR PF01535 576 - 604 1.7e-08 PPR repeat
PPR_1 PF12854 604 - 633 3.1e-09 PPR repeat
PPR_2 PF13041 607 - 650 2.1e-11 PPR repeat family
PPR PF01535 610 - 639 3.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16880
fragaria_vesca FvH4_7g20132
malus_domestica MD01G1131000.v1.1 MD07G1179000.v1.1
prunus_persica Prupe.2G218100_v2.0.a1 Prupe.2G218100_v2.0.a1
pyrus_communis pycom01g13980 pycom07g17270
rosa_chinensis RchiOBHm_Chr1g0364091
rosa_laevigata RLG00000027517
rosa_multiflora Rmu_sc0000071.1_g000021 Rmu_sc0011704.1_g000001
rosa_roxburghii Rroxscaffold_4G00292650
rosa_rugosa Rorug01G0313500
rosa_samantha Rh1AG320900 Rh1BG284100 Rh1CG300500 Rh1DG315500
rosa_wichuraiana Rw1G028450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
AarI CACCTGC 2 cut(s) 572, 934
AasI GACNNNNNNGTC 1 cut(s) 434
Acc36I ACCTGC 4 cut(s) 38, 572, 934, 1591
Acc65I GGTACC 1 cut(s) 594
AccB1I GGYRCC 1 cut(s) 594
AciI CCGC 5 cut(s) 176, 384, 409, 535, 1492
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 2 cut(s) 530, 1907
AfaI GTAC 2 cut(s) 596, 995
AflIII ACRYGT 2 cut(s) 885, 1832
AgeI ACCGGT 1 cut(s) 597
AgsI TTSAA 9 cut(s) 27, 826, 931, 953, 1075, 1396, 1485, 1687, 1816
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 9 cut(s) 74, 783, 1243, 1453, 1518, 1576, 1693, 1883, 1891
AluI AGCT 9 cut(s) 74, 783, 1243, 1453, 1518, 1576, 1693, 1883, 1891
Alw26I GTCTC 1 cut(s) 1042
Ama87I CYCGRG 1 cut(s) 834
AoxI GGCC 6 cut(s) 264, 270, 453, 831, 1146, 1557
ApaI GGGCCC 1 cut(s) 268
ApeKI GCWGC 4 cut(s) 33, 71, 509, 976
ApoI RAATTY 1 cut(s) 755
AseI ATTAAT 1 cut(s) 579
AsiGI ACCGGT 1 cut(s) 597
Asp700I GAANNNNTTC 1 cut(s) 1811
Asp718I GGTACC 1 cut(s) 594
AspS9I GGNCC 7 cut(s) 264, 265, 271, 427, 454, 600, 832
AsuC2I CCSGG 4 cut(s) 425, 493, 835, 836
AsuHPI GGTGA 3 cut(s) 325, 553, 958
AsuII TTCGAA 1 cut(s) 1910
AvaI CYCGRG 1 cut(s) 834
AvaII GGWCC 2 cut(s) 427, 600
BaeGI GKGCMC 2 cut(s) 268, 771
BanI GGYRCC 1 cut(s) 594
BanII GRGCYC 2 cut(s) 268, 1647
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 4 cut(s) 20, 83, 521, 963
BccI CCATC 9 cut(s) 212, 913, 1057, 1109, 1267, 1277, 1372, 1393, 1712
BciT130I CCWGG 1 cut(s) 269
BclI TGATCA 1 cut(s) 1530
BcnI CCSGG 4 cut(s) 425, 493, 835, 836
BcoDI GTCTC 1 cut(s) 1042
BfaI CTAG 4 cut(s) 224, 624, 1697, 1709
BfmI CTRYAG 1 cut(s) 729
BfuAI ACCTGC 4 cut(s) 38, 572, 934, 1591
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 6 cut(s) 34, 72, 384, 510, 536, 977
BlsI GCNGC 6 cut(s) 35, 73, 385, 511, 537, 978
Bme1390I CCNGG 5 cut(s) 269, 425, 493, 835, 836
Bme18I GGWCC 2 cut(s) 427, 600
BmeT110I CYCGRG 1 cut(s) 834
BmgT120I GGNCC 7 cut(s) 264, 265, 271, 427, 454, 600, 832
BmiI GGNNCC 4 cut(s) 266, 429, 596, 1525
BmrFI CCNGG 5 cut(s) 269, 425, 493, 835, 836
BmsI GCATC 8 cut(s) 187, 343, 715, 752, 1071, 1158, 1529, 1918
BpiI GAAGAC 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 1949
Bpu14I TTCGAA 1 cut(s) 1910
BpuMI CCSGG 4 cut(s) 425, 493, 835, 836
Bsa29I ATCGAT 1 cut(s) 1669
BsaAI YACGTR 2 cut(s) 888, 1098
BsaBI GATNNNNATC 1 cut(s) 1403
BsaJI CCNNGG 3 cut(s) 66, 267, 834
BsaWI WCCGGW 2 cut(s) 258, 597
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 2 cut(s) 806, 1254
Bse3DI GCAATG 2 cut(s) 945, 1614
Bse8I GATNNNNATC 1 cut(s) 1403
BseBI CCWGG 1 cut(s) 269
BseCI ATCGAT 1 cut(s) 1669
BseDI CCNNGG 3 cut(s) 66, 267, 834
BseGI GGATG 5 cut(s) 730, 753, 1252, 1544, 1588
BseJI GATNNNNATC 1 cut(s) 1403
BseMI GCAATG 2 cut(s) 945, 1614
BseMII CTCAG 1 cut(s) 1037
BseNI ACTGG 2 cut(s) 806, 1254
BseRI GAGGAG 9 cut(s) 95, 202, 236, 299, 308, 356, 1052, 1147, 1178
BseSI GKGCMC 2 cut(s) 268, 771
BseXI GCAGC 4 cut(s) 20, 83, 521, 963
BseYI CCCAGC 2 cut(s) 231, 1610
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 6 cut(s) 266, 272, 455, 833, 1148, 1559
BshNI GGYRCC 1 cut(s) 594
BshTI ACCGGT 1 cut(s) 597
BshVI ATCGAT 1 cut(s) 1669
BsiEI CGRYCG 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 834
BsiSI CCGG 6 cut(s) 259, 425, 492, 598, 835, 980
BslFI GGGAC 3 cut(s) 422, 851, 1697
BsmAI GTCTC 1 cut(s) 1042
BsmFI GGGAC 3 cut(s) 422, 851, 1697
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 6 cut(s) 266, 272, 455, 833, 1148, 1559
BsoBI CYCGRG 1 cut(s) 834
Bsp119I TTCGAA 1 cut(s) 1910
Bsp120I GGGCCC 1 cut(s) 264
Bsp1286I GDGCHC 3 cut(s) 268, 771, 1647
Bsp143I GATC 5 cut(s) 388, 400, 1530, 1666, 1670
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 5 cut(s) 176, 384, 409, 535, 1492
BspANI GGCC 6 cut(s) 266, 272, 455, 833, 1148, 1559
BspCNI CTCAG 1 cut(s) 1038
BspDI ATCGAT 1 cut(s) 1669
BspLI GGNNCC 4 cut(s) 266, 429, 596, 1525
BspMI ACCTGC 4 cut(s) 38, 572, 934, 1591
BspT104I TTCGAA 1 cut(s) 1910
BspT107I GGYRCC 1 cut(s) 594
BsrDI GCAATG 2 cut(s) 945, 1614
BsrFI RCCGGY 1 cut(s) 597
BsrI ACTGG 2 cut(s) 806, 1254
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 3 cut(s) 66, 267, 834
BssMI GATC 5 cut(s) 388, 400, 1530, 1666, 1670
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 4 cut(s) 58, 436, 971, 1633
Bst6I CTCTTC 4 cut(s) 254, 335, 1428, 1848
BstBAI YACGTR 2 cut(s) 888, 1098
BstBI TTCGAA 1 cut(s) 1910
BstC8I GCNNGC 2 cut(s) 642, 767
BstDEI CTNAG 3 cut(s) 1046, 1488, 1949
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 5 cut(s) 730, 753, 1252, 1544, 1588
BstKTI GATC 5 cut(s) 391, 403, 1533, 1669, 1673
BstMAI GTCTC 1 cut(s) 1042
BstMBI GATC 5 cut(s) 388, 400, 1530, 1666, 1670
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 68, 541
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 2 cut(s) 644, 1836
BstSCI CCNGG 5 cut(s) 267, 423, 491, 833, 834
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 2 cut(s) 268, 771
BstV1I GCAGC 4 cut(s) 20, 83, 521, 963
BstV2I GAAGAC 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 1669
BsuRI GGCC 6 cut(s) 266, 272, 455, 833, 1148, 1559
BsuTUI ATCGAT 1 cut(s) 1669
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 364
BtsCI GGATG 5 cut(s) 730, 753, 1252, 1544, 1588
BtsI GCAGTG 1 cut(s) 1447
BtsIMutI CAGTG 3 cut(s) 1261, 1447, 1629
BveI ACCTGC 4 cut(s) 38, 572, 934, 1591
Cac8I GCNNGC 2 cut(s) 642, 767
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 7 cut(s) 264, 265, 271, 427, 454, 600, 832
Cfr9I CCCGGG 1 cut(s) 834
ClaI ATCGAT 1 cut(s) 1669
Csp6I GTAC 2 cut(s) 595, 994
CspAI ACCGGT 1 cut(s) 597
CviAII CATG 8 cut(s) 67, 641, 853, 1534, 1745, 1757, 1833, 1902
CviQI GTAC 2 cut(s) 595, 994
DdeI CTNAG 3 cut(s) 1046, 1488, 1949
DpnI GATC 5 cut(s) 390, 402, 1532, 1668, 1672
DpnII GATC 5 cut(s) 388, 400, 1530, 1666, 1670
DraI TTTAAA 1 cut(s) 1363
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 4 cut(s) 254, 335, 1428, 1848
EarI CTCTTC 4 cut(s) 254, 335, 1428, 1848
Eco130I CCWWGG 1 cut(s) 66
Eco147I AGGCCT 1 cut(s) 1148
Eco24I GRGCYC 2 cut(s) 268, 1647
Eco47I GGWCC 2 cut(s) 427, 600
Eco57I CTGAAG 2 cut(s) 530, 1907
Eco88I CYCGRG 1 cut(s) 834
EcoO109I RGGNCCY 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 755
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 2 cut(s) 268, 1647
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 8 cut(s) 70, 644, 856, 1537, 1748, 1760, 1836, 1905
FaqI GGGAC 3 cut(s) 422, 851, 1697
FatI CATG 8 cut(s) 66, 640, 852, 1533, 1744, 1756, 1832, 1901
FauI CCCGC 1 cut(s) 1485
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 1530
Fnu4HI GCNGC 6 cut(s) 34, 72, 384, 510, 536, 977
FokI GGATG 5 cut(s) 737, 760, 1259, 1551, 1595
FriOI GRGCYC 2 cut(s) 268, 1647
Fsp4HI GCNGC 6 cut(s) 34, 72, 384, 510, 536, 977
FspBI CTAG 4 cut(s) 224, 624, 1697, 1709
GluI GCNGC 6 cut(s) 34, 72, 384, 510, 536, 977
GsaI CCCAGC 2 cut(s) 235, 1614
HaeIII GGCC 6 cut(s) 266, 272, 455, 833, 1148, 1559
HapII CCGG 6 cut(s) 259, 425, 492, 598, 835, 980
Hin1II CATG 8 cut(s) 70, 644, 856, 1537, 1748, 1760, 1836, 1905
HindIII AAGCTT 1 cut(s) 1889
HinfI GANTC 3 cut(s) 859, 953, 1139
HpaII CCGG 6 cut(s) 259, 425, 492, 598, 835, 980
HphI GGTGA 3 cut(s) 325, 553, 958
Hpy166II GTNNAC 1 cut(s) 1290
Hpy188I TCNGA 8 cut(s) 183, 352, 576, 761, 876, 1047, 1144, 1887
Hpy188III TCNNGA 9 cut(s) 127, 439, 461, 624, 1003, 1295, 1505, 1656, 1697
Hpy8I GTNNAC 1 cut(s) 1290
HpyAV CCTTC 6 cut(s) 445, 467, 808, 925, 1229, 1585
HpyCH4III ACNGT 4 cut(s) 58, 436, 971, 1633
HpyCH4IV ACGT 3 cut(s) 887, 992, 1097
HpyCH4V TGCA 7 cut(s) 36, 509, 567, 943, 1196, 1335, 1440
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 541
HpyF3I CTNAG 3 cut(s) 1046, 1488, 1949
HpySE526I ACGT 3 cut(s) 887, 992, 1097
Hsp92II CATG 8 cut(s) 70, 644, 856, 1537, 1748, 1760, 1836, 1905
KpnI GGTACC 1 cut(s) 598
Ksp22I TGATCA 1 cut(s) 1530
Kzo9I GATC 5 cut(s) 388, 400, 1530, 1666, 1670
LmnI GCTCC 2 cut(s) 79, 1134
Lsp1109I GCAGC 4 cut(s) 20, 83, 521, 963
LweI GCATC 8 cut(s) 187, 343, 715, 752, 1071, 1158, 1529, 1918
MaeI CTAG 4 cut(s) 224, 624, 1697, 1709
MaeII ACGT 3 cut(s) 887, 992, 1097
MaeIII GTNAC 7 cut(s) 52, 1093, 1198, 1303, 1442, 1748, 1933
MalI GATC 5 cut(s) 390, 402, 1532, 1668, 1672
MboI GATC 5 cut(s) 388, 400, 1530, 1666, 1670
MhlI GDGCHC 3 cut(s) 268, 771, 1647
MluCI AATT 5 cut(s) 374, 553, 755, 1635, 1740
MmeI TCCRAC 1 cut(s) 828
MroXI GAANNNNTTC 1 cut(s) 1811
MspI CCGG 6 cut(s) 259, 425, 492, 598, 835, 980
MspR9I CCNGG 5 cut(s) 269, 425, 493, 835, 836
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 68, 541
NciI CCSGG 4 cut(s) 425, 493, 835, 836
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 481
NdeII GATC 5 cut(s) 388, 400, 1530, 1666, 1670
NlaIII CATG 8 cut(s) 70, 644, 856, 1537, 1748, 1760, 1836, 1905
NlaIV GGNNCC 4 cut(s) 266, 429, 596, 1525
NmuCI GTSAC 4 cut(s) 52, 1093, 1303, 1442
NspI RCATGY 2 cut(s) 644, 1836
NspV TTCGAA 1 cut(s) 1910
PaeI GCATGC 1 cut(s) 644
PaqCI CACCTGC 2 cut(s) 572, 934
PceI AGGCCT 1 cut(s) 1148
PciI ACATGT 1 cut(s) 1832
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 1811
PfeI GAWTC 3 cut(s) 859, 953, 1139
PfoI TCCNGGA 1 cut(s) 491
PinAI ACCGGT 1 cut(s) 597
PkrI GCNGC 6 cut(s) 35, 73, 385, 511, 537, 978
Ple19I CGATCG 1 cut(s) 391
Ppu21I YACGTR 2 cut(s) 888, 1098
PscI ACATGT 1 cut(s) 1832
PshBI ATTAAT 1 cut(s) 579
PsiI TTATAA 1 cut(s) 1311
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 2 cut(s) 231, 1610
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 4 cut(s) 266, 429, 596, 1525
PspOMI GGGCCC 1 cut(s) 264
PspPI GGNCC 7 cut(s) 264, 265, 271, 427, 454, 600, 832
PvuI CGATCG 1 cut(s) 391
RsaI GTAC 2 cut(s) 596, 995
RsaNI GTAC 2 cut(s) 595, 994
SatI GCNGC 6 cut(s) 34, 72, 384, 510, 536, 977
Sau3AI GATC 5 cut(s) 388, 400, 1530, 1666, 1670
Sau96I GGNCC 7 cut(s) 264, 265, 271, 427, 454, 600, 832
ScrFI CCNGG 5 cut(s) 269, 425, 493, 835, 836
SduI GDGCHC 3 cut(s) 268, 771, 1647
SfaNI GCATC 8 cut(s) 187, 343, 715, 752, 1071, 1158, 1529, 1918
SfcI CTRYAG 1 cut(s) 729
SfuI TTCGAA 1 cut(s) 1910
SinI GGWCC 2 cut(s) 427, 600
SmaI CCCGGG 1 cut(s) 836
SphI GCATGC 1 cut(s) 644
Sse9I AATT 5 cut(s) 374, 553, 755, 1635, 1740
SseBI AGGCCT 1 cut(s) 1148
SsiI CCGC 5 cut(s) 176, 384, 409, 535, 1492
SspMI CTAG 4 cut(s) 224, 624, 1697, 1709
StuI AGGCCT 1 cut(s) 1148
StyD4I CCNGG 5 cut(s) 267, 423, 491, 833, 834
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 4 cut(s) 58, 436, 971, 1633
TaiI ACGT 3 cut(s) 890, 995, 1100
TaqI TCGA 7 cut(s) 449, 611, 862, 1455, 1669, 1677, 1910
TasI AATT 5 cut(s) 374, 553, 755, 1635, 1740
TauI GCSGC 2 cut(s) 386, 538
TfiI GAWTC 3 cut(s) 859, 953, 1139
TscAI CASTG 3 cut(s) 1261, 1447, 1636
TseFI GTSAC 4 cut(s) 52, 1093, 1303, 1442
TseI GCWGC 4 cut(s) 33, 71, 509, 976
Tsp45I GTSAC 4 cut(s) 52, 1093, 1303, 1442
TspGWI ACGGA 1 cut(s) 979
TspMI CCCGGG 1 cut(s) 834
TspRI CASTG 3 cut(s) 1261, 1447, 1636
VpaK11BI GGWCC 2 cut(s) 427, 600
VspI ATTAAT 1 cut(s) 579
XapI RAATTY 1 cut(s) 755
XbaI TCTAGA 2 cut(s) 623, 1696
XceI RCATGY 2 cut(s) 644, 1836
XmaI CCCGGG 1 cut(s) 834
XmnI GAANNNNTTC 1 cut(s) 1811
XspI CTAG 4 cut(s) 224, 624, 1697, 1709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.