Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0011910

Physical Location & Seq

Genomic Coordinates Forward (+)

12343231 .. 12345629

Gene Structure 2399 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027517

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGACCAACCCAACACCTCGAGAGCAACCCCAAATCCTGGAAACCATCACGAGCCTCTTAATCTCCACCAAAAACCCACAACAACTCCAATCCCTTAAACCCTACATTCCCCACCTAACCGAGCCCCTCCTCCTCTCTCTCCTCCGCTCCAGAACCCTAGCCTCCCACCCCTCCACTCTCCTCTCCTTCTTCCACTGGGCCCAGGCCCACATCCCCCTCCTCTCCCAAACCCCTGGCCCCCTCCTCACCCTCCTCCCCTCTCTCTTCTCCCACAACAAATTCGCCGACGCCAAAACCCTCCTCGTCCAATTCATCTCCTCCGATTCCCAAAACACCCTCCACCGCCTCATCCTCCACCCAGACGACGCCGTTCCGCGCCGCTGCAAGGCCCTCCTCGACACCTCCATCGGCGCCTATGTCCACGTCGGAAAGCCCCACCTCGCCGCGCAGCTCTTCGCGAAAATGAAGAAGCTCCGGTACCGGCCGAATCTCCTCACCTGCAACACTCTGCTCAATGCTCTGGTAAGGTGTGACTCGTCTCATTCCATTGCAATGTGTGTAGATATAGTTAGGGATGCCATTGGACTAGGGGTTACTGTTAATACCGGCACTTTTAACATTTTGATTTACGGGCATTGCTTGGGGAATAAGTTTAGGGATGCCGTCGATTTGTTGAGCAAGATGAATGAGTTTGGATGCCAGCCTGATAATGTGAGCTATAATACGATTTTGGATTGGTTGTGTAAGAAGGGGAAGTTGAGTGAGGCGAGGGAGTTGTTGATGGATATGAAGTTACGAGGGGTGTTGCCGAATAGGAACACGTATAATATTTTGGTTTTCGGGTATTGTAAGATGGGGTGGTTGAGGGAGGCGGTGCAGGTGGTGGAGTTGATGACAGAGAGTAATTTGTTGCCGGATGTTTGGACGTATAATATGTTGATTAGTGGGTTGTGTGGAGCGGGCAGGATGGAGGAGGCGATAAGGCTGAGGGATGAGATGGAGAAGTTGAAGCTGGTGCCGGATGTTGTCACGTATAATACGTTGATTAACGGGTATTTTGAGGGGAACGATGATTCTAAGGCGTTGGAGTTGCTTGAGGAGATGCGTGAACGGGGAGTGAGAGAAAATGAAGTTACTCACAATATAATGGTGAAGTGGTATTGTAAGGAAGGGAAGATGGATGAAGCAAGAGATACTATTAGGAAGATGAAGGAAGAGGGCTTTGCTCCTTGTTCTATCACTTACAATACTTTGATCAACGGGTATTGCAAAGCAGGGAAAATGGAAGAAGCATATAGGGTGATGGATGAGATGTTTAGGAAAGGTTTGGATATGAACACTTTTACCCTTAATACTATTCTTCATGCATTTTGTAGTCAGAAGAAGCTAGACAAGGCATTTACATTGTTCCATACTTCCGTTGAGAGGGGTTATATACTTGATGAGGTGACCTATGGAACTCTGATCATGGGATGCTTTAAGAACGAAAATCCAGACAAGGCTTTGGAGCTTTGGGAAGAGATGAAGGAGAAACAGATCCTTCCTAGCATTGTCACCTACAACTCTATAATTGGAGGCCTATGCCAGTCTGGGAAAACTGATCAAGCACTTGATAAGTTGAATGAGCTTGTAACGACGGATCTAATCCCTGATGAAACTACATACAACACGATAATTCATGGCTATTGCCGTGAGGGTCTGGTTGAGAAAGCATTTGAGTTCCACAACAAAATGGTTGAGAAATCGTTCAAGCCAGATGTCTTTACATGTAATATTCTTCTTTCTGGACTATGCAGTCAGGGTATGTTAGAAAAAGCTCTCAAGCTTTTTAACACCTGGATTTCTAAGGGAAAAGACATTGATGCAGTTACTTACAACATATTGATATCCGGCCTTTGCAAAGAAAGGAGGTTTGGTGATGTTTTTGGTCTTTATGCTGACATGATAGAAAAGAAATTATCTCCGGACCGGTATACATACAGTGCCATTCTCGGTGCACTTACCAATACTGGTGAGATTAAGGAAGCAGAGGAGTTTATATTGAAAATGGGTGAAACGGGGAACTTACATGATTGGTCCTTGAAAATAGACAAGGGGCCAGATGGGGTGACCTGTGAATCTTCAGTAGAATCTGATTCAACCACACTAGCTTATTCAGATCAGATCAATCAGTTGTGTACGCAAGGTAAATACAAGGATGCAATCCACATTTTGCTAGAGTCAAAGCAGAAAGTGACAACTTGGGCTATCTTCCACAATGTCTTTGATAATGAGGGGCATAATGATATAGTCCTCGCCTCCTGGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

770

Amino Acids

86.99

Weight (kDa)

6.1

Isoelectric Point (pI)

28.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	136 - 176	8.7e-07	PPR repeat family
PPR_1	PF12854	197 - 228	6.9e-06	PPR repeat
PPR_2	PF13041	201 - 242	4.5e-09	PPR repeat family
PPR_1	PF12854	232 - 264	6.4e-10	PPR repeat
PPR_2	PF13041	235 - 284	1.5e-17	PPR repeat family
PPR	PF01535	238 - 268	6.3e-07	PPR repeat
PPR_3	PF13812	259 - 315	4.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	266 - 298	2.7e-10	PPR repeat
PPR_2	PF13041	274 - 318	4.5e-12	PPR repeat family
PPR	PF01535	274 - 302	7e-06	PPR repeat
PPR_1	PF12854	302 - 334	5.2e-13	PPR repeat
PPR_2	PF13041	305 - 352	2.3e-17	PPR repeat family
PPR	PF01535	309 - 336	4.5e-07	PPR repeat
PPR_3	PF13812	329 - 388	2.3e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	338 - 369	2.8e-09	PPR repeat
PPR_2	PF13041	340 - 389	1.4e-15	PPR repeat family
PPR	PF01535	343 - 373	1.7e-07	PPR repeat
PPR_long	PF17177	360 - 465	2.5e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	372 - 404	2.2e-06	PPR repeat
PPR_2	PF13041	376 - 410	3.2e-07	PPR repeat family
PPR	PF01535	378 - 408	2.4e-07	PPR repeat
TPR_24	PF23276	396 - 507	2.5e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	407 - 438	3.3e-13	PPR repeat
PPR_2	PF13041	410 - 458	1e-17	PPR repeat family
PPR	PF01535	413 - 443	4.6e-11	PPR repeat
PPR_1	PF12854	441 - 471	2.1e-06	PPR repeat
PPR_2	PF13041	446 - 494	4.5e-10	PPR repeat family
PPR_long	PF17177	458 - 592	4e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	469 - 525	5e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	477 - 509	2.9e-07	PPR repeat
PPR_2	PF13041	481 - 529	1.3e-15	PPR repeat family
PPR	PF01535	483 - 512	2.6e-08	PPR repeat
PPR_1	PF12854	512 - 542	5.5e-08	PPR repeat
PPR_2	PF13041	515 - 557	1e-09	PPR repeat family
TPR_24	PF23276	531 - 647	1e-05	Fungal tetratrico peptide repeats
PPR_1	PF12854	548 - 579	1.1e-12	PPR repeat
PPR_2	PF13041	550 - 598	3.9e-19	PPR repeat family
PPR	PF01535	554 - 582	3.7e-09	PPR repeat
PPR_1	PF12854	582 - 611	3.1e-08	PPR repeat
PPR_2	PF13041	585 - 634	7.6e-16	PPR repeat family
PPR_1	PF12854	618 - 639	6.3e-06	PPR repeat
PPR_2	PF13041	621 - 667	3.8e-14	PPR repeat family
PPR	PF01535	623 - 652	4.8e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011910)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16880
fragaria_vesca	FvH4_7g20132
malus_domestica	MD01G1131000.v1.1 MD07G1179000.v1.1
prunus_persica	Prupe.2G218100_v2.0.a1 Prupe.2G218100_v2.0.a1
pyrus_communis	pycom01g13980 pycom07g17270
rosa_chinensis	RchiOBHm_Chr1g0364091
rosa_laevigata	RLG00000027517
rosa_multiflora	Rmu_sc0000071.1_g000021 Rmu_sc0011704.1_g000001
rosa_roxburghii	Rroxscaffold_4G00292650
rosa_rugosa	Rorug01G0313500
rosa_samantha	Rh1AG320900 Rh1BG284100 Rh1CG300500 Rh1DG315500
rosa_wichuraiana	Rw1G028450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	506, 868
AasI	GACNNNNNNGTC	1 cut(s)	1794
Acc36I	ACCTGC	2 cut(s)	506, 868
Acc65I	GGTACC	1 cut(s)	477
AccB1I	GGYRCC	3 cut(s)	410, 477, 1015
AccB7I	CCANNNNNTGG	1 cut(s)	37
AccBSI	CCGCTC	2 cut(s)	147, 959
AccI	GTMKAC	1 cut(s)	1973
AccII	CGCG	3 cut(s)	376, 446, 458
AccIII	TCCGGA	1 cut(s)	1963
AciI	CCGC	7 cut(s)	145, 343, 374, 379, 444, 872, 959
AclWI	GGATC	2 cut(s)	1531, 1647
AcoI	YGGCCR	1 cut(s)	482
AcsI	RAATTY	1 cut(s)	278
AcuI	CTGAAG	1 cut(s)	2106
AcyI	GRCGYC	3 cut(s)	288, 366, 411
AfaI	GTAC	2 cut(s)	479, 2179
AfiI	CCNNNNNNNGG	3 cut(s)	37, 385, 1425
AflIII	ACRYGT	2 cut(s)	819, 1766
AgeI	ACCGGT	1 cut(s)	1968
AgsI	TTSAA	6 cut(s)	1009, 1621, 1750, 2044, 2083, 2139
AjiI	CACGTC	1 cut(s)	424
AjnI	CCWGG	5 cut(s)	36, 201, 232, 1835, 2300
AjuI	GAANNNNNNNTTGG	2 cut(s)	1896, 1928
Alw21I	GWGCWC	1 cut(s)	1999
Alw26I	GTCTC	1 cut(s)	543
Alw44I	GTGCAC	1 cut(s)	1995
AlwI	GGATC	2 cut(s)	1531, 1647
Ama87I	CYCGRG	1 cut(s)	18
Aor13HI	TCCGGA	1 cut(s)	1963
AoxI	GGCC	8 cut(s)	198, 204, 235, 387, 482, 1576, 1891, 2096
ApaI	GGGCCC	1 cut(s)	202
ApaLI	GTGCAC	1 cut(s)	1995
ApeKI	GCWGC	2 cut(s)	381, 448
ApoI	RAATTY	1 cut(s)	278
AsiGI	ACCGGT	1 cut(s)	1968
Asp700I	GAANNNNTTC	1 cut(s)	1745
Asp718I	GGTACC	1 cut(s)	477
AspLEI	GCGC	3 cut(s)	378, 413, 448
AspS9I	GGNCC	8 cut(s)	198, 199, 205, 236, 388, 1966, 2076, 2096
AvaI	CYCGRG	1 cut(s)	18
AvaII	GGWCC	2 cut(s)	1966, 2076
BaeGI	GKGCMC	2 cut(s)	202, 1999
BanI	GGYRCC	3 cut(s)	410, 477, 1015
BanII	GRGCYC	2 cut(s)	126, 202
BarI	GAAGNNNNNNTAC	2 cut(s)	461, 493
BauI	CACGAG	1 cut(s)	49
Bbv12I	GWGCWC	1 cut(s)	1999
BbvCI	CCTCAGC	1 cut(s)	986
BbvI	GCAGC	2 cut(s)	368, 460
BccI	CCATC	9 cut(s)	53, 413, 775, 847, 961, 991, 1171, 1297, 2096
BceAI	ACGGC	3 cut(s)	353, 647, 1674
BciT130I	CCWGG	5 cut(s)	38, 203, 234, 1837, 2302
BclI	TGATCA	3 cut(s)	1254, 1464, 1600
BcoDI	GTCTC	1 cut(s)	543
BfaI	CTAG	6 cut(s)	158, 587, 1388, 1545, 2147, 2216
BfoI	RGCGCY	1 cut(s)	414
BfuAI	ACCTGC	2 cut(s)	506, 868
BisI	GCNGC	4 cut(s)	379, 382, 444, 449
BlsI	GCNGC	4 cut(s)	380, 383, 445, 450
Bme1390I	CCNGG	5 cut(s)	38, 203, 234, 1837, 2302
Bme18I	GGWCC	2 cut(s)	1966, 2076
BmeT110I	CYCGRG	1 cut(s)	18
BmgBI	CACGTC	1 cut(s)	424
BmgT120I	GGNCC	8 cut(s)	198, 199, 205, 236, 388, 1966, 2076, 2096
BmiI	GGNNCC	6 cut(s)	200, 238, 412, 479, 1017, 2097
BmrFI	CCNGG	5 cut(s)	38, 203, 234, 1837, 2302
BmrI	ACTGGG	1 cut(s)	205
BmsI	GCATC	7 cut(s)	565, 649, 686, 1092, 1463, 1852, 2188
BmuI	ACTGGG	1 cut(s)	205
BpmI	CTGGAG	1 cut(s)	133
Bpu10I	CCTNAGC	1 cut(s)	986
BpuEI	CTTGAG	2 cut(s)	1115, 1805
BsaAI	YACGTR	2 cut(s)	822, 1032
BsaHI	GRCGYC	3 cut(s)	288, 366, 411
BsaJI	CCNNGG	3 cut(s)	201, 232, 2301
BsaWI	WCCGGW	3 cut(s)	474, 1963, 1968
BsaXI	ACNNNNNCTCC	4 cut(s)	69, 99, 1900, 1930
Bsc4I	CCNNNNNNNGG	3 cut(s)	37, 385, 1425
Bse118I	RCCGGY	3 cut(s)	480, 605, 1968
Bse1I	ACTGG	3 cut(s)	200, 1585, 2014
Bse3DI	GCAATG	3 cut(s)	546, 558, 634
BseAI	TCCGGA	1 cut(s)	1963
BseBI	CCWGG	5 cut(s)	38, 203, 234, 1837, 2302
BseDI	CCNNGG	3 cut(s)	201, 232, 2301
BseLI	CCNNNNNNNGG	3 cut(s)	37, 385, 1425
BseMI	GCAATG	3 cut(s)	546, 558, 634
BseMII	CTCAG	1 cut(s)	977
BseNI	ACTGG	3 cut(s)	200, 1585, 2014
BseSI	GKGCMC	2 cut(s)	202, 1999
BseX3I	CGGCCG	1 cut(s)	482
BseXI	GCAGC	2 cut(s)	368, 460
BsgI	GTGCAG	1 cut(s)	896
Bsh1236I	CGCG	3 cut(s)	376, 446, 458
Bsh1285I	CGRYCG	1 cut(s)	485
BshFI	GGCC	8 cut(s)	200, 206, 237, 389, 484, 1578, 1893, 2098
BshNI	GGYRCC	3 cut(s)	410, 477, 1015
BshTI	ACCGGT	1 cut(s)	1968
BsiEI	CGRYCG	1 cut(s)	485
BsiHKAI	GWGCWC	1 cut(s)	1999
BsiHKCI	CYCGRG	1 cut(s)	18
BsiSI	CCGG	8 cut(s)	475, 481, 606, 914, 1019, 1890, 1964, 1969
BslI	CCNNNNNNNGG	3 cut(s)	37, 385, 1425
BsmAI	GTCTC	1 cut(s)	543
BsmBI	CGTCTC	1 cut(s)	543
BsnI	GGCC	8 cut(s)	200, 206, 237, 389, 484, 1578, 1893, 2098
BsoBI	CYCGRG	1 cut(s)	18
Bsp120I	GGGCCC	1 cut(s)	198
Bsp1286I	GDGCHC	3 cut(s)	126, 202, 1999
Bsp13I	TCCGGA	1 cut(s)	1963
Bsp143I	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
Bsp68I	TCGCGA	1 cut(s)	458
BspACI	CCGC	7 cut(s)	145, 343, 374, 379, 444, 872, 959
BspANI	GGCC	8 cut(s)	200, 206, 237, 389, 484, 1578, 1893, 2098
BspCNI	CTCAG	1 cut(s)	978
BspEI	TCCGGA	1 cut(s)	1963
BspFNI	CGCG	3 cut(s)	376, 446, 458
BspLI	GGNNCC	6 cut(s)	200, 238, 412, 479, 1017, 2097
BspMI	ACCTGC	2 cut(s)	506, 868
BspPI	GGATC	2 cut(s)	1531, 1647
BspQI	GCTCTTC	1 cut(s)	458
BspT107I	GGYRCC	3 cut(s)	410, 477, 1015
BsrBI	CCGCTC	2 cut(s)	147, 959
BsrDI	GCAATG	3 cut(s)	546, 558, 634
BsrFI	RCCGGY	3 cut(s)	480, 605, 1968
BsrI	ACTGG	3 cut(s)	200, 1585, 2014
BssAI	RCCGGY	3 cut(s)	480, 605, 1968
BssECI	CCNNGG	3 cut(s)	201, 232, 2301
BssMI	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
BssNAI	GTATAC	1 cut(s)	1974
BssNI	GRCGYC	3 cut(s)	288, 366, 411
BssSI	CACGAG	1 cut(s)	49
Bst1107I	GTATAC	1 cut(s)	1974
Bst2BI	CACGAG	1 cut(s)	49
Bst2UI	CCWGG	5 cut(s)	38, 203, 234, 1837, 2302
Bst4CI	ACNGT	2 cut(s)	598, 1982
Bst6I	CTCTTC	4 cut(s)	269, 458, 1209, 1512
BstACI	GRCGYC	3 cut(s)	288, 366, 411
BstBAI	YACGTR	2 cut(s)	822, 1032
BstC8I	GCNNGC	2 cut(s)	701, 961
BstDEI	CTNAG	3 cut(s)	986, 1077, 1845
BstEII	GGTNACC	2 cut(s)	1447, 2107
BstFNI	CGCG	3 cut(s)	376, 446, 458
BstH2I	RGCGCY	1 cut(s)	414
BstHHI	GCGC	3 cut(s)	378, 413, 448
BstKTI	GATC	7 cut(s)	1257, 1467, 1539, 1603, 1642, 2161, 2166
BstMAI	GTCTC	1 cut(s)	543
BstMBI	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
BstMCI	CGRYCG	1 cut(s)	485
BstNI	CCWGG	5 cut(s)	38, 203, 234, 1837, 2302
BstNSI	RCATGY	1 cut(s)	1770
BstPI	GGTNACC	2 cut(s)	1447, 2107
BstSCI	CCNGG	5 cut(s)	36, 201, 232, 1835, 2300
BstSLI	GKGCMC	2 cut(s)	202, 1999
BstUI	CGCG	3 cut(s)	376, 446, 458
BstV1I	GCAGC	2 cut(s)	368, 460
BstX2I	RGATCY	2 cut(s)	1536, 1639
BstXI	CCANNNNNNTGG	1 cut(s)	233
BstYI	RGATCY	2 cut(s)	1536, 1639
BstZ17I	GTATAC	1 cut(s)	1974
BstZI	CGGCCG	1 cut(s)	482
BsuRI	GGCC	8 cut(s)	200, 206, 237, 389, 484, 1578, 1893, 2098
BtrI	CACGTC	1 cut(s)	424
BtsIMutI	CAGTG	2 cut(s)	193, 1987
BtuMI	TCGCGA	1 cut(s)	458
BveI	ACCTGC	2 cut(s)	506, 868
Cac8I	GCNNGC	2 cut(s)	701, 961
CfoI	GCGC	3 cut(s)	378, 413, 448
Cfr10I	RCCGGY	3 cut(s)	480, 605, 1968
Cfr13I	GGNCC	8 cut(s)	198, 199, 205, 236, 388, 1966, 2076, 2096
CpoI	CGGWCCG	1 cut(s)	1966
CseI	GACGC	2 cut(s)	296, 374
Csp6I	GTAC	2 cut(s)	478, 2178
CspAI	ACCGGT	1 cut(s)	1968
CspI	CGGWCCG	1 cut(s)	1966
CviAII	CATG	6 cut(s)	1364, 1468, 1679, 1767, 1942, 2069
CviQI	GTAC	2 cut(s)	478, 2178
DdeI	CTNAG	3 cut(s)	986, 1077, 1845
DinI	GGCGCC	1 cut(s)	412
DpnI	GATC	7 cut(s)	1256, 1466, 1538, 1602, 1641, 2160, 2165
DpnII	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
DrdI	GACNNNNNNGTC	1 cut(s)	1794
DseDI	GACNNNNNNGTC	1 cut(s)	1794
EaeI	YGGCCR	1 cut(s)	482
EagI	CGGCCG	1 cut(s)	482
Eam1104I	CTCTTC	4 cut(s)	269, 458, 1209, 1512
EarI	CTCTTC	4 cut(s)	269, 458, 1209, 1512
EclXI	CGGCCG	1 cut(s)	482
Eco147I	AGGCCT	1 cut(s)	1578
Eco24I	GRGCYC	2 cut(s)	126, 202
Eco32I	GATATC	1 cut(s)	1887
Eco47I	GGWCC	2 cut(s)	1966, 2076
Eco52I	CGGCCG	1 cut(s)	482
Eco57I	CTGAAG	1 cut(s)	2106
Eco88I	CYCGRG	1 cut(s)	18
Eco91I	GGTNACC	2 cut(s)	1447, 2107
EcoO109I	RGGNCCY	1 cut(s)	388
EcoO65I	GGTNACC	2 cut(s)	1447, 2107
EcoRII	CCWGG	5 cut(s)	36, 201, 232, 1835, 2300
EcoRV	GATATC	1 cut(s)	1887
EcoT22I	ATGCAT	1 cut(s)	1369
EcoT38I	GRGCYC	2 cut(s)	126, 202
EgeI	GGCGCC	1 cut(s)	412
EheI	GGCGCC	1 cut(s)	412
Esp3I	CGTCTC	1 cut(s)	543
FaeI	CATG	6 cut(s)	1367, 1471, 1682, 1770, 1945, 2072
FatI	CATG	6 cut(s)	1363, 1467, 1678, 1766, 1941, 2068
FauI	CCCGC	1 cut(s)	952
FbaI	TGATCA	3 cut(s)	1254, 1464, 1600
FblI	GTMKAC	1 cut(s)	1973
Fnu4HI	GCNGC	4 cut(s)	379, 382, 444, 449
FriOI	GRGCYC	2 cut(s)	126, 202
Fsp4HI	GCNGC	4 cut(s)	379, 382, 444, 449
FspBI	CTAG	6 cut(s)	158, 587, 1388, 1545, 2147, 2216
GlaI	GCGC	3 cut(s)	377, 412, 447
GluI	GCNGC	4 cut(s)	379, 382, 444, 449
GsuI	CTGGAG	1 cut(s)	133
HaeII	RGCGCY	1 cut(s)	414
HaeIII	GGCC	8 cut(s)	200, 206, 237, 389, 484, 1578, 1893, 2098
HapII	CCGG	8 cut(s)	475, 481, 606, 914, 1019, 1890, 1964, 1969
HgaI	GACGC	2 cut(s)	296, 374
HhaI	GCGC	3 cut(s)	378, 413, 448
Hin1I	GRCGYC	3 cut(s)	288, 366, 411
Hin1II	CATG	6 cut(s)	1367, 1471, 1682, 1770, 1945, 2072
Hin6I	GCGC	3 cut(s)	376, 411, 446
HinP1I	GCGC	3 cut(s)	376, 411, 446
HindIII	AAGCTT	1 cut(s)	1823
HinfI	GANTC	8 cut(s)	323, 487, 533, 1073, 2117, 2129, 2135, 2219
HpaII	CCGG	8 cut(s)	475, 481, 606, 914, 1019, 1890, 1964, 1969
Hpy166II	GTNNAC	5 cut(s)	421, 1109, 1974, 1997, 2178
Hpy188I	TCNGA	7 cut(s)	322, 428, 1380, 1464, 2134, 2158, 2163
Hpy188III	TCNNGA	7 cut(s)	20, 49, 150, 457, 1493, 1785, 1964
Hpy8I	GTNNAC	5 cut(s)	421, 1109, 1974, 1997, 2178
Hpy99I	CGWCG	5 cut(s)	290, 368, 428, 668, 1639
HpyAV	CCTTC	6 cut(s)	196, 742, 1163, 1204, 1519, 1550
HpyCH4III	ACNGT	2 cut(s)	598, 1982
HpyCH4IV	ACGT	5 cut(s)	423, 821, 926, 1031, 1040
HpyF3I	CTNAG	3 cut(s)	986, 1077, 1845
HpySE526I	ACGT	5 cut(s)	423, 821, 926, 1031, 1040
Hsp92I	GRCGYC	3 cut(s)	288, 366, 411
Hsp92II	CATG	6 cut(s)	1367, 1471, 1682, 1770, 1945, 2072
HspAI	GCGC	3 cut(s)	376, 411, 446
KasI	GGCGCC	1 cut(s)	410
Kpn2I	TCCGGA	1 cut(s)	1963
KpnI	GGTACC	1 cut(s)	481
Ksp22I	TGATCA	3 cut(s)	1254, 1464, 1600
Kzo9I	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
LguI	GCTCTTC	1 cut(s)	458
LmnI	GCTCC	5 cut(s)	152, 477, 956, 1231, 1507
Lsp1109I	GCAGC	2 cut(s)	368, 460
LweI	GCATC	7 cut(s)	565, 649, 686, 1092, 1463, 1852, 2188
MaeI	CTAG	6 cut(s)	158, 587, 1388, 1545, 2147, 2216
MaeII	ACGT	5 cut(s)	423, 821, 926, 1031, 1040
MalI	GATC	7 cut(s)	1256, 1466, 1538, 1602, 1641, 2160, 2165
MbiI	CCGCTC	2 cut(s)	147, 959
MboI	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
MflI	RGATCY	2 cut(s)	1536, 1639
MhlI	GDGCHC	3 cut(s)	126, 202, 1999
MluCI	AATT	6 cut(s)	278, 308, 904, 1569, 1674, 1955
Mly113I	GGCGCC	1 cut(s)	411
MlyI	GAGTC	2 cut(s)	527, 2228
MmeI	TCCRAC	2 cut(s)	406, 1065
Mph1103I	ATGCAT	1 cut(s)	1369
MroI	TCCGGA	1 cut(s)	1963
MroXI	GAANNNNTTC	1 cut(s)	1745
MseI	TTAA	9 cut(s)	59, 96, 600, 615, 1047, 1350, 1479, 1830, 2019
MslI	CAYNNNNRTG	1 cut(s)	551
MspA1I	CMGCKG	1 cut(s)	381
MspI	CCGG	8 cut(s)	475, 481, 606, 914, 1019, 1890, 1964, 1969
MspR9I	CCNGG	5 cut(s)	38, 203, 234, 1837, 2302
MvaI	CCWGG	5 cut(s)	38, 203, 234, 1837, 2302
MvnI	CGCG	3 cut(s)	376, 446, 458
NarI	GGCGCC	1 cut(s)	411
NdeII	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
NlaIII	CATG	6 cut(s)	1367, 1471, 1682, 1770, 1945, 2072
NlaIV	GGNNCC	6 cut(s)	200, 238, 412, 479, 1017, 2097
NmuCI	GTSAC	6 cut(s)	530, 1027, 1447, 1552, 2107, 2233
NruI	TCGCGA	1 cut(s)	458
NsiI	ATGCAT	1 cut(s)	1369
NspI	RCATGY	1 cut(s)	1770
PaeR7I	CTCGAG	1 cut(s)	18
PaqCI	CACCTGC	2 cut(s)	506, 868
PceI	AGGCCT	1 cut(s)	1578
PciI	ACATGT	1 cut(s)	1766
PciSI	GCTCTTC	1 cut(s)	458
PcsI	WCGNNNNNNNCGW	1 cut(s)	1037
PdmI	GAANNNNTTC	1 cut(s)	1745
PfeI	GAWTC	6 cut(s)	323, 487, 1073, 2117, 2129, 2135
PflMI	CCANNNNNTGG	1 cut(s)	37
PfoI	TCCNGGA	1 cut(s)	36
PinAI	ACCGGT	1 cut(s)	1968
PkrI	GCNGC	4 cut(s)	380, 383, 445, 450
PleI	GAGTC	2 cut(s)	527, 2227
PluTI	GGCGCC	1 cut(s)	414
PpsI	GAGTC	2 cut(s)	527, 2227
Ppu21I	YACGTR	2 cut(s)	822, 1032
PscI	ACATGT	1 cut(s)	1766
Psp6I	CCWGG	5 cut(s)	36, 201, 232, 1835, 2300
PspEI	GGTNACC	2 cut(s)	1447, 2107
PspGI	CCWGG	5 cut(s)	36, 201, 232, 1835, 2300
PspN4I	GGNNCC	6 cut(s)	200, 238, 412, 479, 1017, 2097
PspOMI	GGGCCC	1 cut(s)	198
PspPI	GGNCC	8 cut(s)	198, 199, 205, 236, 388, 1966, 2076, 2096
PsuI	RGATCY	2 cut(s)	1536, 1639
RruI	TCGCGA	1 cut(s)	458
RsaI	GTAC	2 cut(s)	479, 2179
RsaNI	GTAC	2 cut(s)	478, 2178
RseI	CAYNNNNRTG	1 cut(s)	551
Rsr2I	CGGWCCG	1 cut(s)	1966
RsrII	CGGWCCG	1 cut(s)	1966
SapI	GCTCTTC	1 cut(s)	458
SaqAI	TTAA	9 cut(s)	59, 96, 600, 615, 1047, 1350, 1479, 1830, 2019
SatI	GCNGC	4 cut(s)	379, 382, 444, 449
Sau3AI	GATC	7 cut(s)	1254, 1464, 1536, 1600, 1639, 2158, 2163
Sau96I	GGNCC	8 cut(s)	198, 199, 205, 236, 388, 1966, 2076, 2096
SchI	GAGTC	2 cut(s)	527, 2228
ScrFI	CCNGG	5 cut(s)	38, 203, 234, 1837, 2302
SduI	GDGCHC	3 cut(s)	126, 202, 1999
SfaNI	GCATC	7 cut(s)	565, 649, 686, 1092, 1463, 1852, 2188
SfoI	GGCGCC	1 cut(s)	412
Sfr274I	CTCGAG	1 cut(s)	18
SinI	GGWCC	2 cut(s)	1966, 2076
SlaI	CTCGAG	1 cut(s)	18
SmiMI	CAYNNNNRTG	1 cut(s)	551
SmlI	CTYRAG	3 cut(s)	18, 1094, 1820
SmoI	CTYRAG	3 cut(s)	18, 1094, 1820
Sse9I	AATT	6 cut(s)	278, 308, 904, 1569, 1674, 1955
SseBI	AGGCCT	1 cut(s)	1578
SsiI	CCGC	7 cut(s)	145, 343, 374, 379, 444, 872, 959
SspDI	GGCGCC	1 cut(s)	410
SspI	AATATT	2 cut(s)	829, 1774
SspMI	CTAG	6 cut(s)	158, 587, 1388, 1545, 2147, 2216
StuI	AGGCCT	1 cut(s)	1578
StyD4I	CCNGG	5 cut(s)	36, 201, 232, 1835, 2300
TaaI	ACNGT	2 cut(s)	598, 1982
TaiI	ACGT	5 cut(s)	426, 824, 929, 1034, 1043
TaqI	TCGA	3 cut(s)	19, 396, 666
TasI	AATT	6 cut(s)	278, 308, 904, 1569, 1674, 1955
TauI	GCSGC	2 cut(s)	381, 446
TfiI	GAWTC	6 cut(s)	323, 487, 1073, 2117, 2129, 2135
Tru1I	TTAA	9 cut(s)	59, 96, 600, 615, 1047, 1350, 1479, 1830, 2019
Tru9I	TTAA	9 cut(s)	59, 96, 600, 615, 1047, 1350, 1479, 1830, 2019
TscAI	CASTG	2 cut(s)	200, 1987
TseFI	GTSAC	6 cut(s)	530, 1027, 1447, 1552, 2107, 2233
TseI	GCWGC	2 cut(s)	381, 448
Tsp45I	GTSAC	6 cut(s)	530, 1027, 1447, 1552, 2107, 2233
TspGWI	ACGGA	2 cut(s)	1408, 1652
TspRI	CASTG	2 cut(s)	200, 1987
Van91I	CCANNNNNTGG	1 cut(s)	37
VneI	GTGCAC	1 cut(s)	1995
VpaK11BI	GGWCC	2 cut(s)	1966, 2076
XapI	RAATTY	1 cut(s)	278
XceI	RCATGY	1 cut(s)	1770
XhoI	CTCGAG	1 cut(s)	18
XmiI	GTMKAC	1 cut(s)	1973
XmnI	GAANNNNTTC	1 cut(s)	1745
XspI	CTAG	6 cut(s)	158, 587, 1388, 1545, 2147, 2216
Zsp2I	ATGCAT	1 cut(s)	1369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.