MD01G1134100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013288
Physical Location & Seq
Reverse (-)
24365544 .. 24366227
684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1134100.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGGAGAGAAAGAGAAGAAGATCAGTAACAACAAGAAGAACAAGAAGCAAAAACACCAACACCCAAATGACCAAACCACAAAAACAGCCTCAGATTTCTCATTCAAGCCAAGCTCTGATGTCAAGGGCCTACGGTTTGGAGGCCAGTTCGTCGTCAAATCCTTCACAATCCGGCGAGCAAGGCCACTGGAGCTTTTCCAACTCCTTTCTTTCCCACCCAGCAAACCCAGCAACAATAAGCTTCCGTTTCCTTCCACCACAGCTTTTCTCCCCACAAACTTCACAATCTTAGCGCACCACGCCTGGCACACCCTCACATTAGGCCTCGGCACCAAGACGTCCAAGGTGGTTCTCTTCGTTTTCGAAACAGAGGGCATGAAGGCCGCCGTGGACCGCCTTTGGCCGCCGGAAATCCCACTTGGGGAAGTGAACAAGCGGCTCATCAGAGGGCTGAGTGGCTGTGAGATGGCTCGATTTAAATTTAGAAAAGGGTGCATTACTTTTTATGTGTATGCAGTTAGGCAAGTTGGAAACTTGGGTTTCTCAAGTGCTGATGATCTCAGAACAATTTTGCAGTCTGTGGTGGCGCTTCAAGATTTCTTGGATCACACTGCTATGCTTGCCATGCCTAACCAGAGATCCATTAGCTATGCAAATGCTCATCCTGTGGCCATGGCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

25.46

Weight (kDa)

10.11

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7851 PF25236 30 - 216 3e-107 Domain of unknown function (DUF7851)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55420
fragaria_vesca FvH4_7g22470
malus_domestica MD01G1134100.v1.1 MD07G1198300.v1.1
prunus_persica Prupe.2G235300_v2.0.a1
pyrus_communis pycom01g15490 pycom07g18540
rosa_chinensis RchiOBHm_Chr1g0367081
rosa_laevigata RLG00000027301
rosa_multiflora Rmu_sc0003467.1_g000026 Rmu_sc0007424.1_g000002
rosa_roxburghii Rroxscaffold_4G00289640
rosa_rugosa Rorug01G0334300
rosa_samantha Rh1AG341200 Rh1BG301700 Rh1CG317900 Rh1DG334100
rosa_wichuraiana Rw1G030310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 329
AciI CCGC 4 cut(s) 384, 394, 404, 436
AclWI GGATC 2 cut(s) 612, 633
AcoI YGGCCR 2 cut(s) 401, 669
AcsI RAATTY 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 2 cut(s) 106, 593
AjnI CCWGG 1 cut(s) 302
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AluBI AGCT 5 cut(s) 114, 193, 241, 263, 648
AluI AGCT 5 cut(s) 114, 193, 241, 263, 648
AlwI GGATC 2 cut(s) 612, 633
AoxI GGCC 7 cut(s) 127, 142, 182, 322, 381, 401, 669
ApoI RAATTY 1 cut(s) 479
AspLEI GCGC 2 cut(s) 295, 589
AspS9I GGNCC 2 cut(s) 127, 391
AsuII TTCGAA 1 cut(s) 363
AvaII GGWCC 1 cut(s) 391
BalI TGGCCA 1 cut(s) 671
BanI GGYRCC 1 cut(s) 329
BccI CCATC 1 cut(s) 460
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 1 cut(s) 304
BfaI CTAG 1 cut(s) 682
BfoI RGCGCY 1 cut(s) 590
BisI GCNGC 3 cut(s) 384, 404, 437
BlsI GCNGC 3 cut(s) 385, 405, 438
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 1 cut(s) 391
BmgT120I GGNCC 2 cut(s) 127, 391
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 529
BsaHI GRCGYC 1 cut(s) 338
BsaJI CCNNGG 4 cut(s) 325, 342, 387, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 145, 192
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 4 cut(s) 325, 342, 387, 672
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMII CTCAG 3 cut(s) 105, 443, 574
BseNI ACTGG 2 cut(s) 145, 192
BseYI CCCAGC 2 cut(s) 218, 227
BshFI GGCC 7 cut(s) 129, 144, 184, 324, 383, 403, 671
BshNI GGYRCC 1 cut(s) 329
BsiSI CCGG 2 cut(s) 172, 407
BslI CCNNNNNNNGG 1 cut(s) 421
BsnI GGCC 7 cut(s) 129, 144, 184, 324, 383, 403, 671
Bsp119I TTCGAA 1 cut(s) 363
Bsp143I GATC 4 cut(s) 21, 556, 604, 638
Bsp19I CCATGG 1 cut(s) 672
BspACI CCGC 4 cut(s) 384, 394, 404, 436
BspANI GGCC 7 cut(s) 129, 144, 184, 324, 383, 403, 671
BspCNI CTCAG 3 cut(s) 104, 444, 573
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 2 cut(s) 612, 633
BspT104I TTCGAA 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 329
BsrI ACTGG 2 cut(s) 145, 192
BssECI CCNNGG 4 cut(s) 325, 342, 387, 672
BssMI GATC 4 cut(s) 21, 556, 604, 638
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 2 cut(s) 342, 672
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 177, 621
BstDEI CTNAG 4 cut(s) 91, 289, 452, 560
BstDSI CCRYGG 2 cut(s) 387, 672
BstF5I GGATG 1 cut(s) 661
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 2 cut(s) 295, 589
BstKTI GATC 4 cut(s) 24, 559, 607, 641
BstMBI GATC 4 cut(s) 21, 556, 604, 638
BstMWI GCNNNNNNNGC 6 cut(s) 181, 190, 228, 299, 620, 625
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstX2I RGATCY 1 cut(s) 638
BstYI RGATCY 1 cut(s) 638
BsuRI GGCC 7 cut(s) 129, 144, 184, 324, 383, 403, 671
BtgI CCRYGG 2 cut(s) 387, 672
BtsCI GGATG 1 cut(s) 661
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 185, 609
Cac8I GCNNGC 2 cut(s) 177, 621
CfoI GCGC 2 cut(s) 295, 589
Cfr13I GGNCC 2 cut(s) 127, 391
CviAII CATG 3 cut(s) 376, 625, 673
DdeI CTNAG 4 cut(s) 91, 289, 452, 560
DpnI GATC 4 cut(s) 23, 558, 606, 640
DpnII GATC 4 cut(s) 21, 556, 604, 638
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 2 cut(s) 401, 669
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Eco130I CCWWGG 2 cut(s) 342, 672
Eco147I AGGCCT 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 342, 672
ErhI CCWWGG 2 cut(s) 342, 672
FaeI CATG 3 cut(s) 379, 628, 676
FaiI YATR 7 cut(s) 377, 507, 513, 617, 626, 651, 674
FatI CATG 3 cut(s) 375, 624, 672
Fnu4HI GCNGC 3 cut(s) 384, 404, 437
FokI GGATG 1 cut(s) 648
Fsp4HI GCNGC 3 cut(s) 384, 404, 437
FspBI CTAG 1 cut(s) 682
GlaI GCGC 2 cut(s) 294, 588
GluI GCNGC 3 cut(s) 384, 404, 437
GsaI CCCAGC 2 cut(s) 222, 231
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 590
HaeIII GGCC 7 cut(s) 129, 144, 184, 324, 383, 403, 671
HapII CCGG 2 cut(s) 172, 407
HhaI GCGC 2 cut(s) 295, 589
Hin1I GRCGYC 1 cut(s) 338
Hin1II CATG 3 cut(s) 379, 628, 676
Hin6I GCGC 2 cut(s) 293, 587
HinP1I GCGC 2 cut(s) 293, 587
HindIII AAGCTT 1 cut(s) 239
HpaII CCGG 2 cut(s) 172, 407
Hpy166II GTNNAC 2 cut(s) 391, 430
Hpy188I TCNGA 4 cut(s) 94, 118, 446, 563
Hpy188III TCNNGA 1 cut(s) 593
Hpy8I GTNNAC 2 cut(s) 391, 430
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 172, 261, 373
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 1 cut(s) 338
HpyCH4V TGCA 4 cut(s) 495, 515, 574, 653
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 190, 228, 299, 620, 625
HpyF3I CTNAG 4 cut(s) 91, 289, 452, 560
HpySE526I ACGT 1 cut(s) 338
Hsp92I GRCGYC 1 cut(s) 338
Hsp92II CATG 3 cut(s) 379, 628, 676
HspAI GCGC 2 cut(s) 293, 587
Kzo9I GATC 4 cut(s) 21, 556, 604, 638
LmnI GCTCC 1 cut(s) 190
MaeI CTAG 1 cut(s) 682
MaeII ACGT 1 cut(s) 338
MaeIII GTNAC 1 cut(s) 26
MalI GATC 4 cut(s) 23, 558, 606, 640
MboI GATC 4 cut(s) 21, 556, 604, 638
MboII GAAGA 4 cut(s) 28, 31, 49, 346
MflI RGATCY 1 cut(s) 638
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 2 cut(s) 479, 567
MluNI TGGCCA 1 cut(s) 671
MmeI TCCRAC 2 cut(s) 223, 508
MnlI CCTC 6 cut(s) 100, 134, 323, 335, 364, 440
Mox20I TGGCCA 1 cut(s) 671
MscI TGGCCA 1 cut(s) 671
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 2 cut(s) 66, 614
Msp20I TGGCCA 1 cut(s) 671
MspI CCGG 2 cut(s) 172, 407
MspR9I CCNGG 1 cut(s) 304
MvaI CCWGG 1 cut(s) 304
MwoI GCNNNNNNNGC 6 cut(s) 181, 190, 228, 299, 620, 625
NcoI CCATGG 1 cut(s) 672
NdeII GATC 4 cut(s) 21, 556, 604, 638
NlaIII CATG 3 cut(s) 379, 628, 676
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 1 cut(s) 306
NspV TTCGAA 1 cut(s) 363
PceI AGGCCT 1 cut(s) 324
PkrI GCNGC 3 cut(s) 385, 405, 438
Psp6I CCWGG 1 cut(s) 302
PspFI CCCAGC 2 cut(s) 218, 227
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 2 cut(s) 127, 391
PsuI RGATCY 1 cut(s) 638
RseI CAYNNNNRTG 2 cut(s) 66, 614
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 3 cut(s) 384, 404, 437
Sau3AI GATC 4 cut(s) 21, 556, 604, 638
Sau96I GGNCC 2 cut(s) 127, 391
ScrFI CCNGG 1 cut(s) 304
SetI ASST 7 cut(s) 116, 195, 243, 265, 341, 348, 650
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 1 cut(s) 391
SmiI ATTTAAAT 1 cut(s) 478
SmiMI CAYNNNNRTG 2 cut(s) 66, 614
SmlI CTYRAG 1 cut(s) 544
SmoI CTYRAG 1 cut(s) 544
Sse9I AATT 2 cut(s) 479, 567
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 4 cut(s) 384, 394, 404, 436
SspMI CTAG 1 cut(s) 682
StuI AGGCCT 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 2 cut(s) 342, 672
SwaI ATTTAAAT 1 cut(s) 478
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 1 cut(s) 341
TaqI TCGA 2 cut(s) 363, 472
TasI AATT 2 cut(s) 479, 567
TauI GCSGC 3 cut(s) 386, 406, 439
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 2 cut(s) 192, 616
TspDTI ATGAA 1 cut(s) 392
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 2 cut(s) 192, 616
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 1 cut(s) 479
XspI CTAG 1 cut(s) 682
ZraI GACGTC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.