pycom01g15490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013288
Physical Location & Seq
Reverse (-)
15330397 .. 15330978
582 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15490.1

Sequence Viewer

Length: 582 bp
ATGGGAGAGAAAGAGAAGAAGATCAGTAACAACAAGAACAAGAAGCAAAAACATCAACACCCAAATGACCAAAACACAAAAACAGCCTCAGATTTCTCATTCAAGCCAAGCTCTGATGTCAAGGGCCTGCGGTTTGGAGGCCAGTTCGTCGTCAAATCCTTCACAATCCGGCGAGCAAGGCCACTGGAGCTTTTCCAACTCCTTTCTTTCCCACCCAGCAAACCCAGCAACAATAAGCTTCCGTTTCCTTCCACCACAGCTTTTCTCCCCACAAACTTCACAATCTTGGCGCACCACGCCTGGCACACCCTCACATTAGGCCTCGGCACCAAGACGTCCAAGGTGGTTCTCTTCGTTTTCGAAACAGAGGGCATGAAGGCCGCCGTGGACCGCCTTTGGCCGCTGGAAATCCCACTTGGGGAAGTGAACAAGCGGCTCATCAGAGGGCTGAGTGGTTGTGAGATGGCTCGATTTAAATTTAGAAAAGGGTGCATTACCTTTTATGTGTATGCAGTTAGGCAAGTTGGAAACTTGGGTTTCTCAAGTGCTGATGATCGATCAGAACAATTTTGCAGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

21.8

Weight (kDa)

10.05

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7851 PF25236 29 - 186 1.3e-88 Domain of unknown function (DUF7851)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55420
fragaria_vesca FvH4_7g22470
malus_domestica MD01G1134100.v1.1 MD07G1198300.v1.1
prunus_persica Prupe.2G235300_v2.0.a1
pyrus_communis pycom01g15490 pycom07g18540
rosa_chinensis RchiOBHm_Chr1g0367081
rosa_laevigata RLG00000027301
rosa_multiflora Rmu_sc0003467.1_g000026 Rmu_sc0007424.1_g000002
rosa_roxburghii Rroxscaffold_4G00289640
rosa_rugosa Rorug01G0334300
rosa_samantha Rh1AG341200 Rh1BG301700 Rh1CG317900 Rh1DG334100
rosa_wichuraiana Rw1G030310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 326
AciI CCGC 5 cut(s) 130, 381, 391, 401, 433
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 1 cut(s) 476
AcyI GRCGYC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 1 cut(s) 299
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 4 cut(s) 111, 190, 238, 260
AluI AGCT 4 cut(s) 111, 190, 238, 260
AoxI GGCC 6 cut(s) 124, 139, 179, 319, 378, 398
ApoI RAATTY 1 cut(s) 476
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 2 cut(s) 124, 388
AsuII TTCGAA 1 cut(s) 360
AvaII GGWCC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 326
BccI CCATC 1 cut(s) 457
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 301
BfmI CTRYAG 1 cut(s) 578
BisI GCNGC 3 cut(s) 381, 401, 434
BlsI GCNGC 3 cut(s) 382, 402, 435
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 388
BmgT120I GGNCC 2 cut(s) 124, 388
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 301
BpmI CTGGAG 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 526
Bsa29I ATCGAT 1 cut(s) 556
BsaHI GRCGYC 1 cut(s) 335
BsaJI CCNNGG 3 cut(s) 322, 339, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 142, 189
BseBI CCWGG 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 3 cut(s) 322, 339, 384
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 102, 440
BseNI ACTGG 2 cut(s) 142, 189
BseYI CCCAGC 2 cut(s) 215, 224
BshFI GGCC 6 cut(s) 126, 141, 181, 321, 380, 400
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 1 cut(s) 556
BsiSI CCGG 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 418
BsnI GGCC 6 cut(s) 126, 141, 181, 321, 380, 400
Bsp119I TTCGAA 1 cut(s) 360
Bsp143I GATC 3 cut(s) 21, 553, 557
BspACI CCGC 5 cut(s) 130, 381, 391, 401, 433
BspANI GGCC 6 cut(s) 126, 141, 181, 321, 380, 400
BspCNI CTCAG 2 cut(s) 101, 441
BspDI ATCGAT 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 328
BspT104I TTCGAA 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 326
BsrI ACTGG 2 cut(s) 142, 189
BssECI CCNNGG 3 cut(s) 322, 339, 384
BssMI GATC 3 cut(s) 21, 553, 557
BssNI GRCGYC 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 360
BstC8I GCNNGC 2 cut(s) 128, 174
BstDEI CTNAG 2 cut(s) 88, 449
BstDSI CCRYGG 1 cut(s) 384
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 3 cut(s) 24, 556, 560
BstMBI GATC 3 cut(s) 21, 553, 557
BstMWI GCNNNNNNNGC 4 cut(s) 178, 187, 225, 296
BstNI CCWGG 1 cut(s) 301
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 1 cut(s) 578
Bsu15I ATCGAT 1 cut(s) 556
BsuRI GGCC 6 cut(s) 126, 141, 181, 321, 380, 400
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 2 cut(s) 128, 174
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 124, 388
ClaI ATCGAT 1 cut(s) 556
CviAII CATG 1 cut(s) 373
DdeI CTNAG 2 cut(s) 88, 449
DpnI GATC 3 cut(s) 23, 555, 559
DpnII GATC 3 cut(s) 21, 553, 557
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 339
Eco147I AGGCCT 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 388
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 1 cut(s) 376
FaiI YATR 3 cut(s) 374, 504, 510
FatI CATG 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 381, 401, 434
Fsp4HI GCNGC 3 cut(s) 381, 401, 434
GlaI GCGC 1 cut(s) 291
GluI GCNGC 3 cut(s) 381, 401, 434
GsaI CCCAGC 2 cut(s) 219, 228
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 6 cut(s) 126, 141, 181, 321, 380, 400
HapII CCGG 1 cut(s) 169
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 1 cut(s) 376
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HindIII AAGCTT 1 cut(s) 236
HpaII CCGG 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 388, 427
Hpy188I TCNGA 4 cut(s) 91, 115, 443, 562
Hpy8I GTNNAC 2 cut(s) 388, 427
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 3 cut(s) 169, 258, 370
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 3 cut(s) 492, 512, 573
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 187, 225, 296
HpyF3I CTNAG 2 cut(s) 88, 449
HpySE526I ACGT 1 cut(s) 335
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 1 cut(s) 376
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 3 cut(s) 21, 553, 557
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 9 cut(s) 140, 155, 170, 182, 229, 238, 286, 313, 389
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 1 cut(s) 26
MalI GATC 3 cut(s) 23, 555, 559
MboI GATC 3 cut(s) 21, 553, 557
MboII GAAGA 3 cut(s) 28, 31, 343
MluCI AATT 2 cut(s) 476, 566
MmeI TCCRAC 2 cut(s) 220, 505
MnlI CCTC 6 cut(s) 97, 131, 320, 332, 361, 437
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 301
MwoI GCNNNNNNNGC 4 cut(s) 178, 187, 225, 296
NdeII GATC 3 cut(s) 21, 553, 557
NlaIII CATG 1 cut(s) 376
NlaIV GGNNCC 1 cut(s) 328
NmeAIII GCCGAG 1 cut(s) 303
NspV TTCGAA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 321
PkrI GCNGC 3 cut(s) 382, 402, 435
Psp6I CCWGG 1 cut(s) 299
PspFI CCCAGC 2 cut(s) 215, 224
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 124, 388
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 3 cut(s) 381, 401, 434
Sau3AI GATC 3 cut(s) 21, 553, 557
Sau96I GGNCC 2 cut(s) 124, 388
ScrFI CCNGG 1 cut(s) 301
SetI ASST 7 cut(s) 113, 192, 240, 262, 338, 345, 500
SfcI CTRYAG 1 cut(s) 578
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 1 cut(s) 388
SmiI ATTTAAAT 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
Sse9I AATT 2 cut(s) 476, 566
SseBI AGGCCT 1 cut(s) 321
SsiI CCGC 5 cut(s) 130, 381, 391, 401, 433
StuI AGGCCT 1 cut(s) 321
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 339
SwaI ATTTAAAT 1 cut(s) 475
TaiI ACGT 1 cut(s) 338
TaqI TCGA 3 cut(s) 360, 469, 556
TasI AATT 2 cut(s) 476, 566
TauI GCSGC 3 cut(s) 383, 403, 436
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 1 cut(s) 189
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 388
XapI RAATTY 1 cut(s) 476
ZraI GACGTC 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.