pycom07g18540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013288
Physical Location & Seq
Reverse (-)
20988134 .. 20989087
954 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18540.1

Sequence Viewer

Length: 864 bp
ATGCAGTGGCAAAACATTAAAACTCCAATACAATATACCATCCAAAGCTATATTTATCATACATTTAACAAAAACCCACCAAGCAGCACCCACCAAAAACCCAAGATATTCCTCCTCTACAAACAAATCATGGGAGAGAAAGAGAAGAAGATCAACAACAAAAACAAGAAGCAAAAACACCAACATCCAAATGACCAAACAACAAAAACAGCGTCAGAATTTTCCTTCAAGCCAAGCTCTGAAGTCAAAGGCCTACGCTTTGGAGGCCAGTTCGTCGTCAAATCCTTCACAATCCGTCGAGCAAGGCCATTGGAGCTTTTCCAACTCCTCTCATTCCCACCCAGCAAACCCAGAAACGATAAGCTTCCTTTTCCTTCCACCACAGCTTTTCTCCCCACAAACTTCACTATCTTGGCGCACCAAGCTTGGCACACCCTCACATTAGGCCTTGGCACCAAGAAGTCCAAGGTGGTTCTGTTCGTTTTCGAAACCGAGGCCATGAAGGCCGCCGTGGACCGCCTTTGGCCGCCGGAAATCCCACTTGGGAAAGTAAACAAGCGGCTCATCAGAGGGCTGAGTGGCTGTGAGATGGCTAGGTTTAAATTCAGAAAAGGGTGCATTACTTTTTATGTGTATGCAGTCAGGCAAGTTGGAAACTTGGGTTTCTCAAGTGCTGATGATCGATCAGAACAATTTTGCAGTCTGTGGTGGCGCTTCAAGATTTCTTGGATCACACTGCTATGCTTGCCATGCCTAACCAGAGAACCATTAGCTGTGCAAATTCTCATCCTGTGGCCATGGCTCACTAGGAAAATTTTCATATTTTTAACTTCTTTTTTCATGAGCATTATTATCTTCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

33.52

Weight (kDa)

10.18

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7851 PF25236 71 - 228 9.9e-88 Domain of unknown function (DUF7851)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55420
fragaria_vesca FvH4_7g22470
malus_domestica MD01G1134100.v1.1 MD07G1198300.v1.1
prunus_persica Prupe.2G235300_v2.0.a1
pyrus_communis pycom01g15490 pycom07g18540
rosa_chinensis RchiOBHm_Chr1g0367081
rosa_laevigata RLG00000027301
rosa_multiflora Rmu_sc0003467.1_g000026 Rmu_sc0007424.1_g000002
rosa_roxburghii Rroxscaffold_4G00289640
rosa_rugosa Rorug01G0334300
rosa_samantha Rh1AG341200 Rh1BG301700 Rh1CG317900 Rh1DG334100
rosa_wichuraiana Rw1G030310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 452
AciI CCGC 4 cut(s) 507, 517, 527, 559
AclWI GGATC 1 cut(s) 737
AcoI YGGCCR 2 cut(s) 524, 794
AcsI RAATTY 4 cut(s) 218, 602, 780, 813
AcuI CTGAAG 1 cut(s) 261
AgsI TTSAA 2 cut(s) 229, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 7 cut(s) 48, 237, 316, 364, 386, 425, 773
AluI AGCT 7 cut(s) 48, 237, 316, 364, 386, 425, 773
AlwI GGATC 1 cut(s) 737
AoxI GGCC 8 cut(s) 250, 265, 305, 445, 495, 504, 524, 794
ApeKI GCWGC 1 cut(s) 84
ApoI RAATTY 4 cut(s) 218, 602, 780, 813
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp700I GAANNNNTTC 1 cut(s) 815
AspLEI GCGC 2 cut(s) 418, 714
AspS9I GGNCC 1 cut(s) 514
AsuII TTCGAA 1 cut(s) 486
AvaII GGWCC 1 cut(s) 514
BalI TGGCCA 1 cut(s) 796
BanI GGYRCC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 96
BccI CCATC 2 cut(s) 47, 583
BceAI ACGGC 1 cut(s) 494
BfaI CTAG 2 cut(s) 594, 807
BfoI RGCGCY 1 cut(s) 715
BglI GCCNNNNNGGC 1 cut(s) 503
BisI GCNGC 4 cut(s) 85, 507, 527, 560
BlsI GCNGC 4 cut(s) 86, 508, 528, 561
Bme18I GGWCC 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 454
Bpu14I TTCGAA 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 652
Bsa29I ATCGAT 1 cut(s) 682
BsaJI CCNNGG 5 cut(s) 448, 465, 492, 510, 797
Bse1I ACTGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 682
BseDI CCNNGG 5 cut(s) 448, 465, 492, 510, 797
BseGI GGATG 3 cut(s) 39, 184, 786
BseMII CTCAG 1 cut(s) 566
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 2 cut(s) 104, 317
BseXI GCAGC 1 cut(s) 96
BseYI CCCAGC 1 cut(s) 341
BshFI GGCC 8 cut(s) 252, 267, 307, 447, 497, 506, 526, 796
BshNI GGYRCC 1 cut(s) 452
BshVI ATCGAT 1 cut(s) 682
BsiSI CCGG 1 cut(s) 530
BsnI GGCC 8 cut(s) 252, 267, 307, 447, 497, 506, 526, 796
Bsp119I TTCGAA 1 cut(s) 486
Bsp143I GATC 4 cut(s) 150, 679, 683, 729
Bsp19I CCATGG 1 cut(s) 797
BspACI CCGC 4 cut(s) 507, 517, 527, 559
BspANI GGCC 8 cut(s) 252, 267, 307, 447, 497, 506, 526, 796
BspCNI CTCAG 1 cut(s) 567
BspDI ATCGAT 1 cut(s) 682
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 1 cut(s) 454
BspPI GGATC 1 cut(s) 737
BspT104I TTCGAA 1 cut(s) 486
BspT107I GGYRCC 1 cut(s) 452
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 5 cut(s) 448, 465, 492, 510, 797
BssMI GATC 4 cut(s) 150, 679, 683, 729
BssT1I CCWWGG 3 cut(s) 448, 465, 797
BstBI TTCGAA 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 746
BstDEI CTNAG 1 cut(s) 575
BstDSI CCRYGG 2 cut(s) 510, 797
BstF5I GGATG 3 cut(s) 39, 184, 786
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 2 cut(s) 418, 714
BstKTI GATC 4 cut(s) 153, 682, 686, 732
BstMBI GATC 4 cut(s) 150, 679, 683, 729
BstMWI GCNNNNNNNGC 6 cut(s) 264, 313, 422, 503, 745, 750
BstV1I GCAGC 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 682
BsuRI GGCC 8 cut(s) 252, 267, 307, 447, 497, 506, 526, 796
BsuTUI ATCGAT 1 cut(s) 682
BtgI CCRYGG 2 cut(s) 510, 797
BtsCI GGATG 3 cut(s) 39, 184, 786
BtsI GCAGTG 2 cut(s) 11, 734
BtsIMutI CAGTG 2 cut(s) 11, 734
Cac8I GCNNGC 1 cut(s) 746
CciI TCATGA 1 cut(s) 840
CfoI GCGC 2 cut(s) 418, 714
Cfr13I GGNCC 1 cut(s) 514
ClaI ATCGAT 1 cut(s) 682
CseI GACGC 1 cut(s) 201
CviAII CATG 5 cut(s) 130, 499, 750, 798, 841
DdeI CTNAG 1 cut(s) 575
DpnI GATC 4 cut(s) 152, 681, 685, 731
DpnII GATC 4 cut(s) 150, 679, 683, 729
DraI TTTAAA 1 cut(s) 601
EaeI YGGCCR 2 cut(s) 524, 794
Eco130I CCWWGG 3 cut(s) 448, 465, 797
Eco147I AGGCCT 2 cut(s) 252, 447
Eco47I GGWCC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 261
EcoT14I CCWWGG 3 cut(s) 448, 465, 797
ErhI CCWWGG 3 cut(s) 448, 465, 797
FaeI CATG 5 cut(s) 133, 502, 753, 801, 844
FatI CATG 5 cut(s) 129, 498, 749, 797, 840
Fnu4HI GCNGC 4 cut(s) 85, 507, 527, 560
FokI GGATG 3 cut(s) 26, 171, 773
Fsp4HI GCNGC 4 cut(s) 85, 507, 527, 560
FspBI CTAG 2 cut(s) 594, 807
GlaI GCGC 2 cut(s) 417, 713
GluI GCNGC 4 cut(s) 85, 507, 527, 560
GsaI CCCAGC 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 8 cut(s) 252, 267, 307, 447, 497, 506, 526, 796
HapII CCGG 1 cut(s) 530
HgaI GACGC 1 cut(s) 201
HhaI GCGC 2 cut(s) 418, 714
Hin1II CATG 5 cut(s) 133, 502, 753, 801, 844
Hin6I GCGC 2 cut(s) 416, 712
HinP1I GCGC 2 cut(s) 416, 712
HindIII AAGCTT 2 cut(s) 362, 423
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 2 cut(s) 514, 553
Hpy188I TCNGA 5 cut(s) 217, 241, 569, 608, 688
Hpy188III TCNNGA 2 cut(s) 718, 841
Hpy8I GTNNAC 2 cut(s) 514, 553
Hpy99I CGWCG 2 cut(s) 278, 300
HpyAV CCTTC 4 cut(s) 235, 295, 384, 496
HpyCH4V TGCA 5 cut(s) 4, 618, 638, 699, 778
HpyF10VI GCNNNNNNNGC 6 cut(s) 264, 313, 422, 503, 745, 750
HpyF3I CTNAG 1 cut(s) 575
Hsp92II CATG 5 cut(s) 133, 502, 753, 801, 844
HspAI GCGC 2 cut(s) 416, 712
Kzo9I GATC 4 cut(s) 150, 679, 683, 729
LmnI GCTCC 1 cut(s) 313
LpnPI CCDG 7 cut(s) 281, 355, 364, 543, 628, 772, 803
Lsp1109I GCAGC 1 cut(s) 96
MaeI CTAG 2 cut(s) 594, 807
MalI GATC 4 cut(s) 152, 681, 685, 731
MboI GATC 4 cut(s) 150, 679, 683, 729
MboII GAAGA 3 cut(s) 157, 160, 847
MlsI TGGCCA 1 cut(s) 796
MluCI AATT 5 cut(s) 218, 602, 692, 780, 813
MluNI TGGCCA 1 cut(s) 796
MmeI TCCRAC 2 cut(s) 346, 631
MnlI CCTC 7 cut(s) 122, 125, 257, 338, 446, 487, 563
Mox20I TGGCCA 1 cut(s) 796
MroXI GAANNNNTTC 1 cut(s) 815
MscI TGGCCA 1 cut(s) 796
MseI TTAA 4 cut(s) 18, 66, 600, 827
MslI CAYNNNNRTG 2 cut(s) 189, 739
Msp20I TGGCCA 1 cut(s) 796
MspI CCGG 1 cut(s) 530
MwoI GCNNNNNNNGC 6 cut(s) 264, 313, 422, 503, 745, 750
NcoI CCATGG 1 cut(s) 797
NdeII GATC 4 cut(s) 150, 679, 683, 729
NlaIII CATG 5 cut(s) 133, 502, 753, 801, 844
NlaIV GGNNCC 1 cut(s) 454
NspV TTCGAA 1 cut(s) 486
PagI TCATGA 1 cut(s) 840
PceI AGGCCT 2 cut(s) 252, 447
PdmI GAANNNNTTC 1 cut(s) 815
PkrI GCNGC 4 cut(s) 86, 508, 528, 561
PspFI CCCAGC 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 1 cut(s) 514
RseI CAYNNNNRTG 2 cut(s) 189, 739
SaqAI TTAA 4 cut(s) 18, 66, 600, 827
SatI GCNGC 4 cut(s) 85, 507, 527, 560
Sau3AI GATC 4 cut(s) 150, 679, 683, 729
Sau96I GGNCC 1 cut(s) 514
SetI ASST 9 cut(s) 50, 239, 318, 366, 388, 427, 471, 599, 775
SfiI GGCCNNNNNGGCC 1 cut(s) 503
SfuI TTCGAA 1 cut(s) 486
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 2 cut(s) 189, 739
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 5 cut(s) 218, 602, 692, 780, 813
SseBI AGGCCT 2 cut(s) 252, 447
SsiI CCGC 4 cut(s) 507, 517, 527, 559
SspMI CTAG 2 cut(s) 594, 807
StuI AGGCCT 2 cut(s) 252, 447
StyI CCWWGG 3 cut(s) 448, 465, 797
TaqI TCGA 3 cut(s) 298, 486, 682
TasI AATT 5 cut(s) 218, 602, 692, 780, 813
TauI GCSGC 3 cut(s) 509, 529, 562
Tru1I TTAA 4 cut(s) 18, 66, 600, 827
Tru9I TTAA 4 cut(s) 18, 66, 600, 827
TscAI CASTG 1 cut(s) 741
TseI GCWGC 1 cut(s) 84
TspDTI ATGAA 3 cut(s) 515, 808, 829
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 741
VpaK11BI GGWCC 1 cut(s) 514
XapI RAATTY 4 cut(s) 218, 602, 780, 813
XmnI GAANNNNTTC 1 cut(s) 815
XspI CTAG 2 cut(s) 594, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.