MD07G1198300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013288
Physical Location & Seq
Reverse (-)
27655254 .. 27655931
678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1198300.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGGAGAGAAAGAGAAGAAGATCAACAACAAAACCAAGAAGCAAAAACACCAACATCCAAATGACCAAACAACAAAAAAAGCGTCAGAATTTTCCTTCAAGCCAAGCTCTGAAGTCAAAGGCCTACGCTTTGGAGGCCAGTTCGTCGTCAAATCCTTCACAATCCGTCGAGCAAGGCCGTTGGAGCTTTTCCAACTCCTCTCATTCCCACCCAGCAAACCCAGCAAAGATAAGCTTCCTTTTCCTTCCACCACAGCTTTTCTCCCCACAAACTTCACTATCTTGGCGCACCAAGCTTGGCACACTCTCACATTAGGCCTTGGCACCAAGAAGTCCAAGGTGGTTCTGTTCGTTTTCGAAACCGAGGCCATGAAGACCGCCGTGGACCGCCTTTGGCAGCCGGAAATCCCACTTGGGGAAGTGAACAAGCGGCTCATCAGAGGGCTGAGTGGCTGTGAGATGGCTAGGTTTAAATTCAGAAAAGGGTGCATTACTTTTTATGTGTATGCAGTCAGGCAAGTTGGAAACTTGGGTTTCTCAAGTGCTGATGATCTCAGAACAATTTTGCAGTCTGTGGTGGCGCTTCAAGATTTCTTGGATCACACTGCTATGCTTGCCATGCCTAACCAGAGAACCATTAGCTATGCAAATGCTCATCCTGTGGCCATGGCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.41

Weight (kDa)

10.1

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7851 PF25236 28 - 214 2.2e-105 Domain of unknown function (DUF7851)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55420
fragaria_vesca FvH4_7g22470
malus_domestica MD01G1134100.v1.1 MD07G1198300.v1.1
prunus_persica Prupe.2G235300_v2.0.a1
pyrus_communis pycom01g15490 pycom07g18540
rosa_chinensis RchiOBHm_Chr1g0367081
rosa_laevigata RLG00000027301
rosa_multiflora Rmu_sc0003467.1_g000026 Rmu_sc0007424.1_g000002
rosa_roxburghii Rroxscaffold_4G00289640
rosa_rugosa Rorug01G0334300
rosa_samantha Rh1AG341200 Rh1BG301700 Rh1CG317900 Rh1DG334100
rosa_wichuraiana Rw1G030310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 323
AciI CCGC 3 cut(s) 378, 388, 430
AclWI GGATC 1 cut(s) 606
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 2 cut(s) 89, 473
AcuI CTGAAG 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 2 cut(s) 100, 587
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 6 cut(s) 108, 187, 235, 257, 296, 642
AluI AGCT 6 cut(s) 108, 187, 235, 257, 296, 642
AlwI GGATC 1 cut(s) 606
AoxI GGCC 6 cut(s) 121, 136, 176, 316, 366, 663
ApeKI GCWGC 1 cut(s) 397
ApoI RAATTY 2 cut(s) 89, 473
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 289, 583
AspS9I GGNCC 1 cut(s) 385
AsuII TTCGAA 1 cut(s) 357
AvaII GGWCC 1 cut(s) 385
BalI TGGCCA 1 cut(s) 665
BanI GGYRCC 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 409
BccI CCATC 1 cut(s) 454
BceAI ACGGC 2 cut(s) 163, 365
BfaI CTAG 2 cut(s) 465, 676
BfoI RGCGCY 1 cut(s) 584
BisI GCNGC 2 cut(s) 398, 431
BlsI GCNGC 2 cut(s) 399, 432
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 380
Bpu14I TTCGAA 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 523
BsaJI CCNNGG 5 cut(s) 319, 336, 363, 381, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 1 cut(s) 139
BseDI CCNNGG 5 cut(s) 319, 336, 363, 381, 666
BseGI GGATG 2 cut(s) 55, 655
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 2 cut(s) 437, 568
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 1 cut(s) 409
BseYI CCCAGC 2 cut(s) 212, 221
BshFI GGCC 6 cut(s) 123, 138, 178, 318, 368, 665
BshNI GGYRCC 1 cut(s) 323
BsiSI CCGG 1 cut(s) 401
BslI CCNNNNNNNGG 1 cut(s) 415
BsnI GGCC 6 cut(s) 123, 138, 178, 318, 368, 665
Bsp119I TTCGAA 1 cut(s) 357
Bsp143I GATC 3 cut(s) 21, 550, 598
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 3 cut(s) 378, 388, 430
BspANI GGCC 6 cut(s) 123, 138, 178, 318, 368, 665
BspCNI CTCAG 2 cut(s) 438, 567
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 1 cut(s) 606
BspT104I TTCGAA 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 323
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 5 cut(s) 319, 336, 363, 381, 666
BssMI GATC 3 cut(s) 21, 550, 598
BssT1I CCWWGG 3 cut(s) 319, 336, 666
BstBI TTCGAA 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 615
BstDEI CTNAG 2 cut(s) 446, 554
BstDSI CCRYGG 2 cut(s) 381, 666
BstF5I GGATG 2 cut(s) 55, 655
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 2 cut(s) 289, 583
BstKTI GATC 3 cut(s) 24, 553, 601
BstMBI GATC 3 cut(s) 21, 550, 598
BstMWI GCNNNNNNNGC 6 cut(s) 135, 184, 222, 293, 614, 619
BstV1I GCAGC 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 380
BsuRI GGCC 6 cut(s) 123, 138, 178, 318, 368, 665
BtgI CCRYGG 2 cut(s) 381, 666
BtsCI GGATG 2 cut(s) 55, 655
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 1 cut(s) 603
Cac8I GCNNGC 1 cut(s) 615
CfoI GCGC 2 cut(s) 289, 583
Cfr13I GGNCC 1 cut(s) 385
CseI GACGC 1 cut(s) 72
CviAII CATG 3 cut(s) 370, 619, 667
DdeI CTNAG 2 cut(s) 446, 554
DpnI GATC 3 cut(s) 23, 552, 600
DpnII GATC 3 cut(s) 21, 550, 598
DraI TTTAAA 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 663
Eco130I CCWWGG 3 cut(s) 319, 336, 666
Eco147I AGGCCT 2 cut(s) 123, 318
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 132
EcoT14I CCWWGG 3 cut(s) 319, 336, 666
ErhI CCWWGG 3 cut(s) 319, 336, 666
FaeI CATG 3 cut(s) 373, 622, 670
FaiI YATR 7 cut(s) 371, 501, 507, 611, 620, 645, 668
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FatI CATG 3 cut(s) 369, 618, 666
Fnu4HI GCNGC 2 cut(s) 398, 431
FokI GGATG 2 cut(s) 42, 642
Fsp4HI GCNGC 2 cut(s) 398, 431
FspBI CTAG 2 cut(s) 465, 676
GlaI GCGC 2 cut(s) 288, 582
GluI GCNGC 2 cut(s) 398, 431
GsaI CCCAGC 2 cut(s) 216, 225
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 6 cut(s) 123, 138, 178, 318, 368, 665
HapII CCGG 1 cut(s) 401
HgaI GACGC 1 cut(s) 72
HhaI GCGC 2 cut(s) 289, 583
Hin1II CATG 3 cut(s) 373, 622, 670
Hin6I GCGC 2 cut(s) 287, 581
HinP1I GCGC 2 cut(s) 287, 581
HindIII AAGCTT 2 cut(s) 233, 294
HpaII CCGG 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 385, 424
Hpy188I TCNGA 5 cut(s) 88, 112, 440, 479, 557
Hpy188III TCNNGA 1 cut(s) 587
Hpy8I GTNNAC 2 cut(s) 385, 424
Hpy99I CGWCG 2 cut(s) 149, 171
HpyAV CCTTC 3 cut(s) 106, 166, 255
HpyCH4V TGCA 4 cut(s) 489, 509, 568, 647
HpyF10VI GCNNNNNNNGC 6 cut(s) 135, 184, 222, 293, 614, 619
HpyF3I CTNAG 2 cut(s) 446, 554
Hsp92II CATG 3 cut(s) 373, 622, 670
HspAI GCGC 2 cut(s) 287, 581
Kzo9I GATC 3 cut(s) 21, 550, 598
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 7 cut(s) 152, 226, 235, 414, 499, 641, 672
Lsp1109I GCAGC 1 cut(s) 409
MaeI CTAG 2 cut(s) 465, 676
MalI GATC 3 cut(s) 23, 552, 600
MboI GATC 3 cut(s) 21, 550, 598
MboII GAAGA 3 cut(s) 28, 31, 385
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 3 cut(s) 89, 473, 561
MluNI TGGCCA 1 cut(s) 665
MmeI TCCRAC 3 cut(s) 162, 217, 502
MnlI CCTC 4 cut(s) 128, 209, 358, 434
Mox20I TGGCCA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 665
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 2 cut(s) 60, 608
Msp20I TGGCCA 1 cut(s) 665
MspI CCGG 1 cut(s) 401
MwoI GCNNNNNNNGC 6 cut(s) 135, 184, 222, 293, 614, 619
NcoI CCATGG 1 cut(s) 666
NdeII GATC 3 cut(s) 21, 550, 598
NlaIII CATG 3 cut(s) 373, 622, 670
NlaIV GGNNCC 1 cut(s) 325
NspV TTCGAA 1 cut(s) 357
PceI AGGCCT 2 cut(s) 123, 318
PkrI GCNGC 2 cut(s) 399, 432
PspFI CCCAGC 2 cut(s) 212, 221
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 60, 608
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 2 cut(s) 398, 431
Sau3AI GATC 3 cut(s) 21, 550, 598
Sau96I GGNCC 1 cut(s) 385
SetI ASST 8 cut(s) 110, 189, 237, 259, 298, 342, 470, 644
SfuI TTCGAA 1 cut(s) 357
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 2 cut(s) 60, 608
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
Sse9I AATT 3 cut(s) 89, 473, 561
SseBI AGGCCT 2 cut(s) 123, 318
SsiI CCGC 3 cut(s) 378, 388, 430
SspMI CTAG 2 cut(s) 465, 676
StuI AGGCCT 2 cut(s) 123, 318
StyI CCWWGG 3 cut(s) 319, 336, 666
TaqI TCGA 2 cut(s) 169, 357
TasI AATT 3 cut(s) 89, 473, 561
TauI GCSGC 1 cut(s) 433
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 610
TseI GCWGC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 1 cut(s) 610
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 2 cut(s) 89, 473
XspI CTAG 2 cut(s) 465, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.