MD01G1135100.v1.1

Elicitor-responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012403
Physical Location & Seq
Forward (+)
24449843 .. 24452665
2823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1135100.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGCAGTTGGGTTATTGGAGGTGAATCTTGTGAGTGCAAAGGGCCTTGGAGAAACCCATTTATTCAGTCGTATGGATCCGTATGTTGTGATTGAATACAAAGGCCAAGAGAGAAAGAGCAGCGTCGCCAGAGAACAAGGAAGCAGTCCGGAGTGGAACGAGAAATTTACGTTCAGGGCAGAATATCCAGGTTCAGGGGGAGAGTATAAGATCACTCTCAAGATCATGGACCAAGACACCTTCACTTCTGATGACTTTATCGGCCAAGCGACAATATACGTGAAAGAGTTATTGGCACTGGGAGTGCAGAATGGAACTGCCGAACAACATCCTCTTTACTATAGCGTTGTTCGCGCTAATAATACTTATCATGGGGAGATCAAAGTCGGCCTAACTTTCACTCCGCAAAACAGGATGACGGGGAACAATCGTTTGGAGGATGGAAGAACAGTGCTGCGTATCCATAGAGAAACAATACGTCTGCAGATGGGGTTGCATTGCAGAGTAGGGTTTGAGTTCGAGTCCGGGCCCACACTCGATCTGTGGGACCCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

186

Amino Acids

20.92

Weight (kDa)

5.75

Isoelectric Point (pI)

25.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 4 - 99 1.2e-20 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55470 AT3G55470
fragaria_vesca FvH4_7g22610
malus_domestica MD01G1135100.v1.1 MD07G1199800.v1.1
prunus_persica Prupe.2G236500_v2.0.a1 Prupe.2G236500_v2.0.a1
pyrus_communis pycom07g18660
rosa_chinensis RchiOBHm_Chr1g0367731
rosa_laevigata RLG00000027267
rosa_multiflora Rmu_co8228921.1_g000001 Rmu_sc0016772.1_g000001
rosa_roxburghii Rroxscaffold_4G00289270
rosa_rugosa Rorug01G0336300
rosa_samantha Rh1AG343400 Rh1BG305200 Rh1CG320800 Rh1DG337400
rosa_wichuraiana Rw1G030560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 354
AccIII TCCGGA 1 cut(s) 148
AciI CCGC 1 cut(s) 404
AclWI GGATC 2 cut(s) 71, 84
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 164
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 1 cut(s) 95
AjnI CCWGG 1 cut(s) 187
AjuI GAANNNNNNNTTGG 4 cut(s) 275, 307, 416, 448
AlwI GGATC 2 cut(s) 71, 84
Aor13HI TCCGGA 1 cut(s) 148
AoxI GGCC 5 cut(s) 43, 103, 262, 388, 527
ApaI GGGCCC 1 cut(s) 531
ApeKI GCWGC 2 cut(s) 120, 454
ApoI RAATTY 1 cut(s) 164
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 5 cut(s) 43, 229, 527, 528, 547
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 2 cut(s) 229, 547
BaeGI GKGCMC 1 cut(s) 531
BamHI GGATCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 531
BbvI GCAGC 2 cut(s) 132, 441
BccI CCATC 2 cut(s) 434, 481
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 470
BcnI CCSGG 1 cut(s) 526
BfmI CTRYAG 2 cut(s) 340, 482
BfuI GTATCC 1 cut(s) 470
BisI GCNGC 2 cut(s) 121, 455
BlsI GCNGC 2 cut(s) 122, 456
Bme1390I CCNGG 2 cut(s) 189, 526
Bme18I GGWCC 2 cut(s) 229, 547
BmgT120I GGNCC 5 cut(s) 43, 229, 527, 528, 547
BmiI GGNNCC 4 cut(s) 78, 529, 548, 549
BmrFI CCNGG 2 cut(s) 189, 526
BmrI ACTGGG 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 526
BsaAI YACGTR 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 496
BseAI TCCGGA 1 cut(s) 148
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 3 cut(s) 328, 420, 445
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 496
BseNI ACTGG 1 cut(s) 303
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 2 cut(s) 132, 441
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 5 cut(s) 45, 105, 264, 390, 529
BsiSI CCGG 2 cut(s) 149, 525
BslI CCNNNNNNNGG 1 cut(s) 194
BsnI GGCC 5 cut(s) 45, 105, 264, 390, 529
Bsp120I GGGCCC 1 cut(s) 527
Bsp1286I GDGCHC 1 cut(s) 531
Bsp13I TCCGGA 1 cut(s) 148
Bsp143I GATC 5 cut(s) 76, 210, 222, 378, 538
BspACI CCGC 1 cut(s) 404
BspANI GGCC 5 cut(s) 45, 105, 264, 390, 529
BspEI TCCGGA 1 cut(s) 148
BspFNI CGCG 1 cut(s) 354
BspLI GGNNCC 4 cut(s) 78, 529, 548, 549
BspMAI CTGCAG 1 cut(s) 486
BspPI GGATC 2 cut(s) 71, 84
BsrDI GCAATG 1 cut(s) 496
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 5 cut(s) 76, 210, 222, 378, 538
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 280
BstF5I GGATG 3 cut(s) 328, 420, 445
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 5 cut(s) 79, 213, 225, 381, 541
BstMBI GATC 5 cut(s) 76, 210, 222, 378, 538
BstMWI GCNNNNNNNGC 1 cut(s) 351
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 2 cut(s) 187, 524
BstSFI CTRYAG 2 cut(s) 340, 482
BstSLI GKGCMC 1 cut(s) 531
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 132, 441
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuI GTATCC 1 cut(s) 470
BsuRI GGCC 5 cut(s) 45, 105, 264, 390, 529
BtsCI GGATG 3 cut(s) 328, 420, 445
BtsIMutI CAGTG 2 cut(s) 296, 456
CfoI GCGC 1 cut(s) 356
Cfr13I GGNCC 5 cut(s) 43, 229, 527, 528, 547
CseI GACGC 1 cut(s) 112
CviAII CATG 3 cut(s) 226, 371, 552
CviJI RGCY 5 cut(s) 45, 105, 264, 390, 529
CviKI_1 RGCY 5 cut(s) 45, 105, 264, 390, 529
DpnI GATC 5 cut(s) 78, 212, 224, 380, 540
DpnII GATC 5 cut(s) 76, 210, 222, 378, 538
EaeI YGGCCR 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 531
Eco47I GGWCC 2 cut(s) 229, 547
EcoO109I RGGNCCY 2 cut(s) 43, 547
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 3 cut(s) 229, 374, 555
FaiI YATR 9 cut(s) 74, 84, 207, 227, 277, 342, 372, 465, 553
FatI CATG 3 cut(s) 225, 370, 551
Fnu4HI GCNGC 2 cut(s) 121, 455
FokI GGATG 3 cut(s) 315, 427, 452
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 2 cut(s) 121, 455
GlaI GCGC 1 cut(s) 355
GluI GCNGC 2 cut(s) 121, 455
HaeIII GGCC 5 cut(s) 45, 105, 264, 390, 529
HapII CCGG 2 cut(s) 149, 525
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 356
Hin1II CATG 3 cut(s) 229, 374, 555
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 2 cut(s) 25, 521
HpaII CCGG 2 cut(s) 149, 525
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 2 cut(s) 149, 220
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 3 cut(s) 170, 279, 478
HpyCH4V TGCA 5 cut(s) 38, 307, 484, 496, 501
HpyF10VI GCNNNNNNNGC 1 cut(s) 351
HpySE526I ACGT 3 cut(s) 170, 279, 478
Hsp92II CATG 3 cut(s) 229, 374, 555
HspAI GCGC 1 cut(s) 354
KflI GGGWCCC 1 cut(s) 547
Kpn2I TCCGGA 1 cut(s) 148
Kzo9I GATC 5 cut(s) 76, 210, 222, 378, 538
LpnPI CCDG 9 cut(s) 142, 160, 162, 174, 180, 201, 284, 397, 538
Lsp1109I GCAGC 2 cut(s) 132, 441
MaeII ACGT 3 cut(s) 170, 279, 478
MalI GATC 5 cut(s) 78, 212, 224, 380, 540
MboI GATC 5 cut(s) 76, 210, 222, 378, 538
MboII GAAGA 1 cut(s) 456
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 1 cut(s) 164
MlyI GAGTC 1 cut(s) 530
MnlI CCTC 3 cut(s) 13, 342, 430
MroI TCCGGA 1 cut(s) 148
MspI CCGG 2 cut(s) 149, 525
MspR9I CCNGG 2 cut(s) 189, 526
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 1 cut(s) 351
NciI CCSGG 1 cut(s) 526
NdeII GATC 5 cut(s) 76, 210, 222, 378, 538
NlaIII CATG 3 cut(s) 229, 374, 555
NlaIV GGNNCC 4 cut(s) 78, 529, 548, 549
PfeI GAWTC 1 cut(s) 25
PkrI GCNGC 2 cut(s) 122, 456
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 280
PpuMI RGGWCCY 1 cut(s) 547
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 4 cut(s) 78, 529, 548, 549
PspOMI GGGCCC 1 cut(s) 527
PspPI GGNCC 5 cut(s) 43, 229, 527, 528, 547
PspPPI RGGWCCY 1 cut(s) 547
PstI CTGCAG 1 cut(s) 486
PsuI RGATCY 1 cut(s) 76
SatI GCNGC 2 cut(s) 121, 455
Sau3AI GATC 5 cut(s) 76, 210, 222, 378, 538
Sau96I GGNCC 5 cut(s) 43, 229, 527, 528, 547
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 189, 526
SduI GDGCHC 1 cut(s) 531
SetI ASST 6 cut(s) 24, 173, 193, 242, 282, 481
SfcI CTRYAG 2 cut(s) 340, 482
SinI GGWCC 2 cut(s) 229, 547
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 1 cut(s) 164
SsiI CCGC 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 187, 524
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 3 cut(s) 173, 282, 481
TaqI TCGA 2 cut(s) 519, 537
TasI AATT 1 cut(s) 164
TfiI GAWTC 1 cut(s) 25
TscAI CASTG 2 cut(s) 303, 456
TseI GCWGC 2 cut(s) 120, 454
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 2 cut(s) 303, 456
VpaK11BI GGWCC 2 cut(s) 229, 547
XapI RAATTY 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.