MD01G1136000.v1.1

Fizzy-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009046
Physical Location & Seq
Reverse (-)
24498075 .. 24501529
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1136000.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGATATCATCACATTCTTCAGACCCAGCAACCCGGAAACTCCTCCTCCAAGCAACCAAGAACCGCATCCAACAGAACCCAGCAATGGAGTCACCTCAAACCAGGAAATCGGGGCTCAACCTCCCCACCGGGATGTCCGAGACACTGCTCCGCCTCGATTCCGTTTCGGGTTCTTTTCGTGCGGTTTCGAGCCTCTCAGCGTCGCCGGGTTCTTGTCGGGCGATTTCGAACCTGTCCTCGCCGTCCAAGTCCTCGACTTGCAGCGACAGATTCATCCCCTGCAGATCCTCGTCGCGGCTTCACACGTTCGGGCTCAACGAGAAGGCCTCGCCGGTGAAAGAAGGAGGCAACGAGGCGTATGCCAGGTTGTTGAAATCGGAGCTTTTCGGGTCTGATTTCGGGTCTTTTTCTCCTGCAGGTGGCGGAGGTGGTGGTGCAGCGTCGCCCATGAGTCCCAGCAAGAACATGTTGCGGTTCAAGACGGAGAACTCGGGCCCGAACTCGCCTTACTCGCCGTCCCTTCTGAGAAATGACAGTGGGTTTTCCAGCGAGACTACGCCTCCCAAGCCTCCTCGCAAGGTCCCCAAGACACCCCATAAGGTTCTGGATGCTCCGTCACTACAAGATGACTTTTACTTGAACCTTGTGGACTGGTCCTCCCAAAATGTCCTTGCAGTTGGACTGGGCACCAGTGTTTATCTCTGGAGTGCATCAAACAGCAAGGTGACAAAACTGTGTGACTTGGGGCCTAATGACGGCGTGTGCTCTGTCCAATGGACACGGGAGGGTTCATACTTGTCGATTGGCACAAGTCTTGGTCGAGTTCAGGTTTGGGATGGGACGCAGTGCAAGAGGGTCCGAACAATGGGCGGGCATCAAACAAGGACCGGAGTCTTGGCGTGGAACTCGCGCATATTAGCTTCAGGGAGCCGAGATCGGAATATACTTCAACACGACCTTCGAGTCCCAGACAACTATATCAGCAAGCTTGTTGGCCACAAGTCTGAGGTATGTGGGCTGAAATGGTCCAACGATGACAGGGAACTTGCATCCGGTGGCAATGATAATCAGCTCTTGGTCTGGAACCAGCAGTCTCAGCAACCGGCTTTGAGACTGACAGAGCACACAGCTGCAGTCAAGGCCATTGCTTGGTCACCCCACCAGAGTGGCCTTCTTGCATCTGGAGGCGGAACTGCTGACAGATGTATTCGGTTCTGGAACACTACAAACGGCCACCAATTGAACAGCATCGACACAGGAAGCCAGGTCTGCAATCTTGCATGGAGTAAGAATGTCAATGAGATAGTCAGCACTCATGGGTATTCCCAGAATCAAATTATGGTGTGGAAGTATCCATCCATGGGAAAGGTTGCCAATCTAACCGGTCATAGCATGCGAGTTTTGTACCTTGCAATGTCACCGGATGGTCAGACAATAGTCACTGGTGCCGGGGATGAGACTCTAAGATTCTGGAACGTCTTTCCATCTGTGAAGACACATACGTCAGTGAAGGATACCGGTCTTTGGTCACTAGGACGAACTCAGATTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914
Pfam Domains
Protein Families
WD40

Protein Analysis

518

Amino Acids

55.96

Weight (kDa)

9.6

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 202 - 491 1.8e-128 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 217 - 315 7.7e-08 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 217 - 315 7.9e-12 WDR19 first beta-propeller
Beta-prop_EML_2 PF23414 220 - 389 4.9e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 223 - 319 1.6e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 227 - 321 6.2e-08 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 248 - 492 3.6e-14 MABP1/WDR62 second WD40 domain
WDR55 PF24796 252 - 496 1.7e-10 WDR55
Beta-prop_WDR3_2nd PF25172 302 - 410 1.9e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 302 - 437 1e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 303 - 493 2.3e-20 Prp19 WD40 domain
WD40 PF00400 327 - 362 2.6e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 328 - 427 1.4e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 328 - 437 5.9e-17 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 329 - 417 3.8e-11 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 329 - 499 1.1e-11 G protein beta WD-40 repeat protein
EIF3I PF24805 330 - 439 5.3e-06 EIF3I
WD40_WDHD1_1st PF24817 333 - 392 1.6e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 333 - 438 4.4e-22 THOC3 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 371 - 499 5.4e-06 MABP1/WDR62 first WD40 domain
Beta-prop_EML PF23409 373 - 494 5.4e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 374 - 494 2.2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 397 - 492 1e-08 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 399 - 494 8.7e-11 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 399 - 501 2e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 421 - 495 3.3e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 442 - 494 4.8e-08 THOC3 beta-propeller domain
WD40 PF00400 457 - 492 2.3e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 407
AasI GACNNNNNNGTC 2 cut(s) 962, 1501
Acc36I ACCTGC 1 cut(s) 407
AccB1I GGYRCC 2 cut(s) 686, 1445
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccII CGCG 2 cut(s) 295, 910
AciI CCGC 8 cut(s) 64, 151, 182, 295, 423, 472, 870, 1188
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 2 cut(s) 994, 1231
AcuI CTGAAG 1 cut(s) 906
AfaI GTAC 1 cut(s) 1406
AfiI CCNNNNNNNGG 8 cut(s) 85, 294, 419, 755, 865, 1149, 1361, 1524
AflIII ACRYGT 2 cut(s) 303, 465
AgeI ACCGGT 2 cut(s) 1382, 1517
AgsI TTSAA 5 cut(s) 373, 478, 640, 950, 1243
AjnI CCWGG 3 cut(s) 101, 362, 1263
AleI CACNNNNGTG 1 cut(s) 1164
AluBI AGCT 5 cut(s) 382, 920, 988, 1072, 1130
AluI AGCT 5 cut(s) 382, 920, 988, 1072, 1130
Alw21I GWGCWC 2 cut(s) 767, 1125
Alw26I GTCTC 5 cut(s) 134, 545, 1098, 1105, 1451
AlwI GGATC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 7 cut(s) 324, 493, 746, 994, 1140, 1168, 1231
ApaI GGGCCC 1 cut(s) 497
ApeKI GCWGC 3 cut(s) 261, 437, 1130
AsiGI ACCGGT 2 cut(s) 1382, 1517
AspLEI GCGC 1 cut(s) 912
AspS9I GGNCC 8 cut(s) 493, 494, 580, 654, 746, 856, 885, 1026
AsuC2I CCSGG 4 cut(s) 34, 130, 207, 1450
AsuHPI GGTGA 5 cut(s) 84, 346, 736, 1146, 1410
AsuII TTCGAA 1 cut(s) 227
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 5 cut(s) 580, 654, 856, 885, 1026
BaeGI GKGCMC 2 cut(s) 497, 689
BalI TGGCCA 1 cut(s) 996
BanI GGYRCC 2 cut(s) 686, 1445
BanII GRGCYC 3 cut(s) 117, 315, 497
BbsI GAAGAC 1 cut(s) 1499
Bbv12I GWGCWC 2 cut(s) 767, 1125
BbvI GCAGC 3 cut(s) 273, 449, 1117
BccI CCATC 4 cut(s) 830, 1363, 1418, 1492
BceAI ACGGC 4 cut(s) 226, 499, 772, 1246
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 3 cut(s) 103, 364, 1265
BciVI GTATCC 2 cut(s) 1362, 1507
BcnI CCSGG 4 cut(s) 34, 130, 207, 1450
BcoDI GTCTC 5 cut(s) 134, 545, 1098, 1105, 1451
BfaI CTAG 1 cut(s) 1532
BfmI CTRYAG 3 cut(s) 280, 414, 1131
BfuAI ACCTGC 1 cut(s) 407
BfuI GTATCC 2 cut(s) 1362, 1507
BisI GCNGC 4 cut(s) 262, 296, 438, 1131
BlsI GCNGC 4 cut(s) 263, 297, 439, 1132
Bme1390I CCNGG 7 cut(s) 34, 103, 130, 207, 364, 1265, 1450
Bme18I GGWCC 5 cut(s) 580, 654, 856, 885, 1026
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 8 cut(s) 493, 494, 580, 654, 746, 856, 885, 1026
BmiI GGNNCC 8 cut(s) 495, 582, 688, 747, 857, 929, 1085, 1447
BmrFI CCNGG 7 cut(s) 34, 103, 130, 207, 364, 1265, 1450
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 7 cut(s) 75, 598, 719, 883, 1058, 1187, 1257
BmuI ACTGGG 1 cut(s) 692
BoxI GACNNNNGTC 2 cut(s) 890, 1436
BpiI GAAGAC 1 cut(s) 1499
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 2 cut(s) 724, 1203
Bpu14I TTCGAA 1 cut(s) 227
BpuMI CCSGG 4 cut(s) 34, 130, 207, 1450
BsaJI CCNNGG 2 cut(s) 1359, 1449
BsaWI WCCGGW 5 cut(s) 887, 1052, 1382, 1420, 1517
BsaXI ACNNNNNCTCC 6 cut(s) 30, 60, 544, 574, 641, 671
Bsc4I CCNNNNNNNGG 8 cut(s) 85, 294, 419, 755, 865, 1149, 1361, 1524
Bse118I RCCGGY 4 cut(s) 331, 1102, 1382, 1517
Bse1I ACTGG 4 cut(s) 656, 687, 690, 1447
Bse3DI GCAATG 4 cut(s) 90, 1066, 1143, 1419
BseBI CCWGG 3 cut(s) 103, 364, 1265
BseDI CCNNGG 2 cut(s) 1359, 1449
BseGI GGATG 9 cut(s) 66, 138, 273, 613, 841, 1049, 1355, 1429, 1459
BseLI CCNNNNNNNGG 8 cut(s) 85, 294, 419, 755, 865, 1149, 1361, 1524
BseMI GCAATG 4 cut(s) 90, 1066, 1143, 1419
BseMII CTCAG 4 cut(s) 210, 515, 996, 1109
BseNI ACTGG 4 cut(s) 656, 687, 690, 1447
BseRI GAGGAG 3 cut(s) 32, 35, 561
BseSI GKGCMC 2 cut(s) 497, 689
BseXI GCAGC 3 cut(s) 273, 449, 1117
BseYI CCCAGC 3 cut(s) 25, 79, 455
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 2 cut(s) 295, 910
BshFI GGCC 7 cut(s) 326, 495, 748, 996, 1142, 1170, 1233
BshNI GGYRCC 2 cut(s) 686, 1445
BshTI ACCGGT 2 cut(s) 1382, 1517
BsiHKAI GWGCWC 2 cut(s) 767, 1125
BsiHKCI CYCGRG 1 cut(s) 490
BslFI GGGAC 5 cut(s) 438, 502, 566, 853, 950
BslI CCNNNNNNNGG 8 cut(s) 85, 294, 419, 755, 865, 1149, 1361, 1524
BsmAI GTCTC 5 cut(s) 134, 545, 1098, 1105, 1451
BsmFI GGGAC 5 cut(s) 438, 502, 566, 853, 950
BsnI GGCC 7 cut(s) 326, 495, 748, 996, 1142, 1170, 1233
BsoBI CYCGRG 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 227
Bsp120I GGGCCC 1 cut(s) 493
Bsp1286I GDGCHC 6 cut(s) 117, 315, 497, 689, 767, 1125
Bsp143I GATC 2 cut(s) 284, 934
Bsp19I CCATGG 1 cut(s) 1359
BspACI CCGC 8 cut(s) 64, 151, 182, 295, 423, 472, 870, 1188
BspANI GGCC 7 cut(s) 326, 495, 748, 996, 1142, 1170, 1233
BspCNI CTCAG 4 cut(s) 209, 516, 997, 1108
BspFNI CGCG 2 cut(s) 295, 910
BspLI GGNNCC 8 cut(s) 495, 582, 688, 747, 857, 929, 1085, 1447
BspMAI CTGCAG 3 cut(s) 284, 418, 1135
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 1 cut(s) 279
BspT104I TTCGAA 1 cut(s) 227
BspT107I GGYRCC 2 cut(s) 686, 1445
BsrDI GCAATG 4 cut(s) 90, 1066, 1143, 1419
BsrFI RCCGGY 4 cut(s) 331, 1102, 1382, 1517
BsrI ACTGG 4 cut(s) 656, 687, 690, 1447
BssAI RCCGGY 4 cut(s) 331, 1102, 1382, 1517
BssECI CCNNGG 2 cut(s) 1359, 1449
BssMI GATC 2 cut(s) 284, 934
BssT1I CCWWGG 1 cut(s) 1359
Bst2UI CCWGG 3 cut(s) 103, 364, 1265
Bst4CI ACNGT 2 cut(s) 536, 735
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 3 cut(s) 872, 986, 1394
BstDEI CTNAG 6 cut(s) 196, 524, 1005, 1095, 1463, 1542
BstDSI CCRYGG 1 cut(s) 1359
BstEII GGTNACC 1 cut(s) 1152
BstF5I GGATG 9 cut(s) 66, 138, 273, 613, 841, 1049, 1355, 1429, 1459
BstFNI CGCG 2 cut(s) 295, 910
BstHHI GCGC 1 cut(s) 912
BstKTI GATC 2 cut(s) 287, 937
BstMAI GTCTC 5 cut(s) 134, 545, 1098, 1105, 1451
BstMBI GATC 2 cut(s) 284, 934
BstMWI GCNNNNNNNGC 5 cut(s) 511, 565, 1096, 1139, 1269
BstNI CCWGG 3 cut(s) 103, 364, 1265
BstNSI RCATGY 2 cut(s) 469, 1396
BstPAI GACNNNNGTC 2 cut(s) 890, 1436
BstPI GGTNACC 1 cut(s) 1152
BstSCI CCNGG 7 cut(s) 32, 101, 128, 205, 362, 1263, 1448
BstSFI CTRYAG 3 cut(s) 280, 414, 1131
BstSLI GKGCMC 2 cut(s) 497, 689
BstUI CGCG 2 cut(s) 295, 910
BstV1I GCAGC 3 cut(s) 273, 449, 1117
BstV2I GAAGAC 1 cut(s) 1499
BstX2I RGATCY 1 cut(s) 284
BstXI CCANNNNNNTGG 1 cut(s) 1166
BstYI RGATCY 1 cut(s) 284
BsuI GTATCC 2 cut(s) 1362, 1507
BsuRI GGCC 7 cut(s) 326, 495, 748, 996, 1142, 1170, 1233
BtgI CCRYGG 1 cut(s) 1359
BtsCI GGATG 9 cut(s) 66, 138, 273, 613, 841, 1049, 1355, 1429, 1459
BtsI GCAGTG 2 cut(s) 143, 851
BtsIMutI CAGTG 6 cut(s) 143, 541, 697, 851, 1440, 1512
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 3 cut(s) 872, 986, 1394
CfoI GCGC 1 cut(s) 912
Cfr10I RCCGGY 4 cut(s) 331, 1102, 1382, 1517
Cfr13I GGNCC 8 cut(s) 493, 494, 580, 654, 746, 856, 885, 1026
CseI GACGC 3 cut(s) 189, 429, 850
Csp6I GTAC 1 cut(s) 1405
CspAI ACCGGT 2 cut(s) 1382, 1517
CviAII CATG 6 cut(s) 448, 466, 1281, 1316, 1360, 1393
CviQI GTAC 1 cut(s) 1405
DdeI CTNAG 6 cut(s) 196, 524, 1005, 1095, 1463, 1542
DpnI GATC 2 cut(s) 286, 936
DpnII GATC 2 cut(s) 284, 934
DrdI GACNNNNNNGTC 2 cut(s) 962, 1501
DseDI GACNNNNNNGTC 2 cut(s) 962, 1501
EaeI YGGCCR 2 cut(s) 994, 1231
EciI GGCGGA 3 cut(s) 140, 438, 1203
Eco130I CCWWGG 1 cut(s) 1359
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 3 cut(s) 117, 315, 497
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 5 cut(s) 580, 654, 856, 885, 1026
Eco57I CTGAAG 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 490
Eco91I GGTNACC 1 cut(s) 1152
EcoO109I RGGNCCY 2 cut(s) 580, 746
EcoO65I GGTNACC 1 cut(s) 1152
EcoRII CCWGG 3 cut(s) 101, 362, 1263
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 1359
EcoT38I GRGCYC 3 cut(s) 117, 315, 497
ErhI CCWWGG 1 cut(s) 1359
FaeI CATG 6 cut(s) 451, 469, 1284, 1319, 1363, 1396
FaqI GGGAC 5 cut(s) 438, 502, 566, 853, 950
FatI CATG 6 cut(s) 447, 465, 1280, 1315, 1359, 1392
FauI CCCGC 1 cut(s) 863
Fnu4HI GCNGC 4 cut(s) 262, 296, 438, 1131
FokI GGATG 9 cut(s) 53, 145, 260, 620, 848, 1036, 1342, 1436, 1466
FriOI GRGCYC 3 cut(s) 117, 315, 497
Fsp4HI GCNGC 4 cut(s) 262, 296, 438, 1131
FspBI CTAG 1 cut(s) 1532
GlaI GCGC 1 cut(s) 911
GluI GCNGC 4 cut(s) 262, 296, 438, 1131
GsaI CCCAGC 3 cut(s) 29, 83, 459
GsuI CTGGAG 2 cut(s) 724, 1203
HaeIII GGCC 7 cut(s) 326, 495, 748, 996, 1142, 1170, 1233
HgaI GACGC 3 cut(s) 189, 429, 850
HhaI GCGC 1 cut(s) 912
Hin1II CATG 6 cut(s) 451, 469, 1284, 1319, 1363, 1396
Hin6I GCGC 1 cut(s) 910
HinP1I GCGC 1 cut(s) 910
HindIII AAGCTT 1 cut(s) 986
HphI GGTGA 5 cut(s) 84, 346, 736, 1146, 1410
Hpy166II GTNNAC 1 cut(s) 649
Hpy188III TCNNGA 7 cut(s) 478, 605, 703, 1081, 1182, 1216, 1471
Hpy8I GTNNAC 1 cut(s) 649
Hpy99I CGWCG 3 cut(s) 205, 295, 445
HpyAV CCTTC 6 cut(s) 316, 335, 530, 968, 1181, 1504
HpyCH4III ACNGT 2 cut(s) 536, 735
HpyCH4IV ACGT 3 cut(s) 305, 1476, 1502
HpyF10VI GCNNNNNNNGC 5 cut(s) 511, 565, 1096, 1139, 1269
HpyF3I CTNAG 6 cut(s) 196, 524, 1005, 1095, 1463, 1542
HpySE526I ACGT 3 cut(s) 305, 1476, 1502
Hsp92II CATG 6 cut(s) 451, 469, 1284, 1319, 1363, 1396
HspAI GCGC 1 cut(s) 910
Kzo9I GATC 2 cut(s) 284, 934
LmnI GCTCC 4 cut(s) 153, 379, 616, 927
Lsp1109I GCAGC 3 cut(s) 273, 449, 1117
LweI GCATC 7 cut(s) 75, 598, 719, 883, 1058, 1187, 1257
MaeI CTAG 1 cut(s) 1532
MaeII ACGT 3 cut(s) 305, 1476, 1502
MaeIII GTNAC 8 cut(s) 90, 615, 724, 737, 1152, 1416, 1438, 1527
MalI GATC 2 cut(s) 286, 936
MboI GATC 2 cut(s) 284, 934
MboII GAAGA 2 cut(s) 9, 1504
MfeI CAATTG 1 cut(s) 1238
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 6 cut(s) 117, 315, 497, 689, 767, 1125
MlsI TGGCCA 1 cut(s) 996
MluCI AATT 2 cut(s) 1238, 1335
MluNI TGGCCA 1 cut(s) 996
MlyI GAGTC 5 cut(s) 98, 460, 900, 972, 1453
MmeI TCCRAC 3 cut(s) 94, 658, 1053
Mox20I TGGCCA 1 cut(s) 996
MscI TGGCCA 1 cut(s) 996
MslI CAYNNNNRTG 2 cut(s) 131, 1164
Msp20I TGGCCA 1 cut(s) 996
MspA1I CMGCKG 1 cut(s) 1130
MspR9I CCNGG 7 cut(s) 34, 103, 130, 207, 364, 1265, 1450
MunI CAATTG 1 cut(s) 1238
MvaI CCWGG 3 cut(s) 103, 364, 1265
MvnI CGCG 2 cut(s) 295, 910
MwoI GCNNNNNNNGC 5 cut(s) 511, 565, 1096, 1139, 1269
NciI CCSGG 4 cut(s) 34, 130, 207, 1450
NcoI CCATGG 1 cut(s) 1359
NdeII GATC 2 cut(s) 284, 934
NlaIII CATG 6 cut(s) 451, 469, 1284, 1319, 1363, 1396
NlaIV GGNNCC 8 cut(s) 495, 582, 688, 747, 857, 929, 1085, 1447
NmeAIII GCCGAG 1 cut(s) 956
NmuCI GTSAC 8 cut(s) 90, 615, 724, 737, 1152, 1416, 1438, 1527
NspI RCATGY 2 cut(s) 469, 1396
NspV TTCGAA 1 cut(s) 227
OliI CACNNNNGTG 1 cut(s) 1164
PaeI GCATGC 1 cut(s) 1396
PaqCI CACCTGC 1 cut(s) 407
PceI AGGCCT 1 cut(s) 326
PciI ACATGT 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfeI GAWTC 5 cut(s) 158, 270, 1330, 1467, 1546
PflMI CCANNNNNTGG 1 cut(s) 1149
PinAI ACCGGT 2 cut(s) 1382, 1517
PkrI GCNGC 4 cut(s) 263, 297, 439, 1132
PleI GAGTC 5 cut(s) 97, 459, 899, 971, 1453
PpsI GAGTC 5 cut(s) 97, 459, 899, 971, 1453
PpuMI RGGWCCY 1 cut(s) 580
PscI ACATGT 1 cut(s) 465
PshAI GACNNNNGTC 2 cut(s) 890, 1436
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 3 cut(s) 101, 362, 1263
PspEI GGTNACC 1 cut(s) 1152
PspFI CCCAGC 3 cut(s) 25, 79, 455
PspGI CCWGG 3 cut(s) 101, 362, 1263
PspN4I GGNNCC 8 cut(s) 495, 582, 688, 747, 857, 929, 1085, 1447
PspOMI GGGCCC 1 cut(s) 493
PspPI GGNCC 8 cut(s) 493, 494, 580, 654, 746, 856, 885, 1026
PspPPI RGGWCCY 1 cut(s) 580
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PstI CTGCAG 3 cut(s) 284, 418, 1135
PsuI RGATCY 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 1130
RsaI GTAC 1 cut(s) 1406
RsaNI GTAC 1 cut(s) 1405
RseI CAYNNNNRTG 2 cut(s) 131, 1164
SatI GCNGC 4 cut(s) 262, 296, 438, 1131
Sau3AI GATC 2 cut(s) 284, 934
Sau96I GGNCC 8 cut(s) 493, 494, 580, 654, 746, 856, 885, 1026
SbfI CCTGCAGG 1 cut(s) 418
SchI GAGTC 5 cut(s) 98, 460, 900, 972, 1453
ScrFI CCNGG 7 cut(s) 34, 103, 130, 207, 364, 1265, 1450
SdaI CCTGCAGG 1 cut(s) 418
SduI GDGCHC 6 cut(s) 117, 315, 497, 689, 767, 1125
SfaNI GCATC 7 cut(s) 75, 598, 719, 883, 1058, 1187, 1257
SfcI CTRYAG 3 cut(s) 280, 414, 1131
SfuI TTCGAA 1 cut(s) 227
SgrAI CRCCGGYG 1 cut(s) 331
SinI GGWCC 5 cut(s) 580, 654, 856, 885, 1026
SmiMI CAYNNNNRTG 2 cut(s) 131, 1164
SphI GCATGC 1 cut(s) 1396
Sse8387I CCTGCAGG 1 cut(s) 418
Sse9I AATT 2 cut(s) 1238, 1335
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 8 cut(s) 64, 151, 182, 295, 423, 472, 870, 1188
SspMI CTAG 1 cut(s) 1532
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 7 cut(s) 32, 101, 128, 205, 362, 1263, 1448
StyI CCWWGG 1 cut(s) 1359
TaaI ACNGT 2 cut(s) 536, 735
TaiI ACGT 3 cut(s) 308, 1479, 1505
TaqI TCGA 9 cut(s) 156, 188, 227, 254, 800, 820, 961, 1251, 1549
TasI AATT 2 cut(s) 1238, 1335
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 5 cut(s) 158, 270, 1330, 1467, 1546
TscAI CASTG 6 cut(s) 150, 541, 697, 851, 1447, 1512
TseFI GTSAC 8 cut(s) 90, 615, 724, 737, 1152, 1416, 1438, 1527
TseI GCWGC 3 cut(s) 261, 437, 1130
Tsp45I GTSAC 8 cut(s) 90, 615, 724, 737, 1152, 1416, 1438, 1527
TspDTI ATGAA 2 cut(s) 262, 780
TspGWI ACGGA 3 cut(s) 151, 497, 603
TspRI CASTG 6 cut(s) 150, 541, 697, 851, 1447, 1512
Van91I CCANNNNNTGG 1 cut(s) 1149
VpaK11BI GGWCC 5 cut(s) 580, 654, 856, 885, 1026
XceI RCATGY 2 cut(s) 469, 1396
XspI CTAG 1 cut(s) 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.