RchiOBHm_Chr1g0367881

Fizzy-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009046
Physical Location & Seq
Reverse (-)
58088275 .. 58091503
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59226

Sequence Viewer

Length: 1578 bp
ATGACCGTTAGAGCACCCAAGTCTTCGTCTTCCTCCTCACTCCAAACCCCCAAAGCCCTCCTTGCTCCCACACAACGAGGCCTTTTCCGATCTTGGGTTTCTTCCTCCTCCATCAATTTTCCAAACCCAGAAATGGATTCACCGCAAACTCCCCGGAAAACCGGGCTCAACCTCCCAACCGGGATGTCCGAGTCGACTCTGCGGCTCGACTCGCTCTCCAGCTCGTTCCGGGGGATCTCGAGCCTCTCGTCGTCGTCGCCGTCGAAGACCTCGACGTGCAGCGACAGGTTCATTCCGTGCCGGTCGTCGTCGAGGCTCCACCAGTTTGGGCTGAACGACAAGGCGTCGCCGGTGAAGGACGGCGGGAACGAGGCCTACCAGAGGCTGTTGAGATCGGAGCTTTTCGGGTCTGATTTCGATTCTTTTTCTTCTCCTGCAGGTGGAGGAGGACAGGCGGCGTCGTCGCCGATTAGTCCCAGCAAGAACATGTTGCGGTTCAAGACGGAGCATTCCGGGCCAAACTCGCCGTATTCGCCGTCGATTTTGAGGCATGATAGTGGGTTTTCGAATGAGAGCTGTACTCCTCCCAAGCCTCCCAGAAAGGTCCCCAAGACCCCCCATAAGGTTCTGGATGCTCCATCACTTCAAGATGACTTTTACTTGAATCTGGTGGACTGGTCCTCGCAAAATGTCCTTGCAGTTGGATTGGGAACCTCAGTTTATTTATGGAATGCATCAAACAGCAAGGTGACAAGACTGTGTGACTTGGGGCCTAATGATGGCGTCTGCTCTGTTCAATGGACCAAGGAGGGTTCATATTTATCAATTGGTACAAACGGTGGCCAAGTTCAGGTTTGGGATGGGACTCAATGCAAGAAGGTCCGAACAATGAGTGGACATCAAACAAGAACTGGTGTTTTGGCATGGAACTCAAGGATACTAGCTTCAGGAAGCCGAGACCGGAACATCCTTCAACATGATATGCGAGTCTCAAATGACTGCATCAACAAGCTTGTTGGCCACAAGTCTGAGGTATGCGGGCTGAAATGGTCCAACGATGACAGGGAACTTGCTTCTGGTGGCAATGATAATCAGCTCTTGGTGTGGAACCAGCACTCTCAGCAACCTGCGTTGAGACTAACAGAGCACACAGCTGCAGTAAAGGCCATTGCTTGGTCACCCCACCAGAGCGGCCTTCTTGCTTCTGGAGGTGGAACTGCTGACAGATGTATACGCTTCTGGAACACTGCAAATGGCCACCAGTTGAACAGCGTTGACACGGGAAGCCAGGTTTGCAATCTCGCATGGAGTAAGAATGTGAATGAGATAGTTAGCACTCATGGATACTCCCAAAACCAAATTATGGTCTGGAAGTATCCATCCATGTCAAAGGTTGCCACTCTAACTGGCCATAGCATGCGAGTTCTATACCTTGCAATGTCACCAGATGGTCAGACAATAGTCACTGGTGCTGGGGATGAAACTCTCAGATTCTGGAATGTGTTTCCTTCGGTAAAAACTCCGACACCATGCAAAGAAACAGGTCTTTGGTCACTGGGACGAACTCAAATCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914
Pfam Domains
Protein Families
WD40

Protein Analysis

525

Amino Acids

57.05

Weight (kDa)

9.43

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 210 - 499 3.1e-129 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 221 - 311 4.4e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 225 - 342 3e-09 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 226 - 323 1e-11 WDR19 first beta-propeller
Beta-prop_WDR3_2nd PF25172 228 - 500 1.3e-18 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 228 - 397 8.3e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 231 - 328 1e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 235 - 347 2.2e-12 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 257 - 500 2.4e-16 MABP1/WDR62 second WD40 domain
WDR55 PF24796 260 - 504 1.3e-10 WDR55
Beta-prop_WDR5 PF25175 310 - 427 5.9e-18 WDR5 beta-propeller domain
WD40_Prp19 PF24814 311 - 500 8.5e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 330 - 504 1.8e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 331 - 445 1.5e-17 WDR3 first beta-propeller domain
WD40 PF00400 333 - 370 5.1e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 333 - 424 1.9e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 334 - 435 3.1e-08 TEP-1 second beta-propeller
EIF3I PF24805 338 - 447 3.4e-06 EIF3I
WD40_WDHD1_1st PF24817 340 - 400 2.6e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 341 - 446 7e-22 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 348 - 500 3.1e-06 WDR19 first beta-propeller
WD40_MABP1-WDR62_1st PF24780 380 - 502 2.8e-06 MABP1/WDR62 first WD40 domain
Beta-prop_EML PF23409 381 - 502 6.9e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 382 - 501 1.4e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 407 - 512 3.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 408 - 501 1.3e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 429 - 502 3.9e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 450 - 501 1.7e-08 THOC3 beta-propeller domain
WD40 PF00400 464 - 500 3.4e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 428
Acc36I ACCTGC 2 cut(s) 428, 1135
AccB7I CCANNNNNTGG 2 cut(s) 1173, 1363
AccBSI CCGCTC 1 cut(s) 1191
AccI GTMKAC 2 cut(s) 194, 1231
AciI CCGC 7 cut(s) 143, 202, 363, 455, 493, 1038, 1191
AclWI GGATC 1 cut(s) 242
AcoI YGGCCR 4 cut(s) 841, 1018, 1255, 1408
AcuI CTGAAG 1 cut(s) 930
AcyI GRCGYC 3 cut(s) 344, 458, 783
AfaI GTAC 2 cut(s) 580, 832
AfiI CCNNNNNNNGG 9 cut(s) 94, 133, 381, 440, 622, 779, 850, 1173, 1363
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 6 cut(s) 499, 647, 664, 797, 974, 1267
AhdI GACNNNNNGTC 1 cut(s) 343
AjiI CACGTC 1 cut(s) 276
AjnI CCWGG 1 cut(s) 1287
AluBI AGCT 7 cut(s) 222, 400, 576, 944, 1012, 1096, 1154
AluI AGCT 7 cut(s) 222, 400, 576, 944, 1012, 1096, 1154
Alw21I GWGCWC 2 cut(s) 16, 1149
Alw26I GTCTC 3 cut(s) 951, 994, 1129
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 2 cut(s) 385, 1494
Ama87I CYCGRG 1 cut(s) 238
ApeKI GCWGC 2 cut(s) 279, 1154
AspS9I GGNCC 7 cut(s) 515, 604, 678, 770, 801, 880, 1050
AsuC2I CCSGG 5 cut(s) 154, 163, 181, 230, 514
AsuHPI GGTGA 5 cut(s) 132, 364, 760, 1170, 1434
AsuII TTCGAA 1 cut(s) 566
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 5 cut(s) 604, 678, 801, 880, 1050
BalI TGGCCA 4 cut(s) 843, 1020, 1257, 1410
BanII GRGCYC 1 cut(s) 168
BbsI GAAGAC 3 cut(s) 15, 21, 272
Bbv12I GWGCWC 2 cut(s) 16, 1149
BbvI GCAGC 2 cut(s) 291, 1141
BccI CCATC 6 cut(s) 119, 646, 773, 854, 1387, 1442
BceAI ACGGC 4 cut(s) 244, 376, 511, 520
BciT130I CCWGG 1 cut(s) 1289
BciVI GTATCC 3 cut(s) 930, 1337, 1386
BcnI CCSGG 5 cut(s) 154, 163, 181, 230, 514
BcoDI GTCTC 3 cut(s) 951, 994, 1129
BfaI CTAG 1 cut(s) 941
BfmI CTRYAG 2 cut(s) 435, 1155
BfuAI ACCTGC 2 cut(s) 428, 1135
BfuI GTATCC 3 cut(s) 930, 1337, 1386
BisI GCNGC 5 cut(s) 203, 280, 456, 1155, 1192
BlsI GCNGC 5 cut(s) 204, 281, 457, 1156, 1193
Bme1390I CCNGG 6 cut(s) 154, 163, 181, 230, 514, 1289
Bme18I GGWCC 5 cut(s) 604, 678, 801, 880, 1050
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 276
BmgT120I GGNCC 7 cut(s) 515, 604, 678, 770, 801, 880, 1050
BmiI GGNNCC 5 cut(s) 317, 606, 712, 771, 1109
BmrFI CCNGG 6 cut(s) 154, 163, 181, 230, 514, 1289
BmrI ACTGGG 1 cut(s) 1565
BmsI GCATC 3 cut(s) 622, 743, 1011
BmuI ACTGGG 1 cut(s) 1565
BoxI GACNNNNGTC 1 cut(s) 1460
BpiI GAAGAC 3 cut(s) 15, 21, 272
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BpmI CTGGAG 2 cut(s) 202, 1227
Bpu14I TTCGAA 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 916
BpuMI CCSGG 5 cut(s) 154, 163, 181, 230, 514
BsaHI GRCGYC 3 cut(s) 344, 458, 783
BsaI GGTCTC 1 cut(s) 951
BsaJI CCNNGG 3 cut(s) 152, 229, 804
BsaWI WCCGGW 1 cut(s) 960
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 9 cut(s) 94, 133, 381, 440, 622, 779, 850, 1173, 1363
Bse118I RCCGGY 2 cut(s) 300, 349
Bse1I ACTGG 7 cut(s) 322, 680, 916, 1261, 1411, 1471, 1560
Bse3DI GCAATG 3 cut(s) 1090, 1167, 1443
BseBI CCWGG 1 cut(s) 1289
BseDI CCNNGG 3 cut(s) 152, 229, 804
BseGI GGATG 6 cut(s) 189, 637, 865, 966, 1379, 1483
BseLI CCNNNNNNNGG 9 cut(s) 94, 133, 381, 440, 622, 779, 850, 1173, 1363
BseMI GCAATG 3 cut(s) 1090, 1167, 1443
BseMII CTCAG 4 cut(s) 729, 1020, 1133, 1501
BseNI ACTGG 7 cut(s) 322, 680, 916, 1261, 1411, 1471, 1560
BseRI GAGGAG 4 cut(s) 25, 97, 459, 573
BseXI GCAGC 2 cut(s) 291, 1141
BseYI CCCAGC 2 cut(s) 476, 1472
BsgI GTGCAG 1 cut(s) 298
Bsh1285I CGRYCG 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 2 cut(s) 16, 1149
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 8 cut(s) 154, 162, 180, 229, 301, 350, 513, 961
BslFI GGGAC 4 cut(s) 459, 590, 877, 1572
BslI CCNNNNNNNGG 9 cut(s) 94, 133, 381, 440, 622, 779, 850, 1173, 1363
BsmAI GTCTC 3 cut(s) 951, 994, 1129
BsmFI GGGAC 4 cut(s) 459, 590, 877, 1572
BsmI GAATGC 2 cut(s) 508, 736
Bso31I GGTCTC 1 cut(s) 951
BsoBI CYCGRG 1 cut(s) 238
Bsp119I TTCGAA 1 cut(s) 566
Bsp1286I GDGCHC 3 cut(s) 16, 168, 1149
Bsp143I GATC 3 cut(s) 89, 234, 392
BspACI CCGC 7 cut(s) 143, 202, 363, 455, 493, 1038, 1191
BspCNI CTCAG 4 cut(s) 728, 1021, 1132, 1500
BspLI GGNNCC 5 cut(s) 317, 606, 712, 771, 1109
BspMAI CTGCAG 2 cut(s) 439, 1159
BspMI ACCTGC 2 cut(s) 428, 1135
BspPI GGATC 1 cut(s) 242
BspT104I TTCGAA 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 951
BsrBI CCGCTC 1 cut(s) 1191
BsrDI GCAATG 3 cut(s) 1090, 1167, 1443
BsrFI RCCGGY 2 cut(s) 300, 349
BsrI ACTGG 7 cut(s) 322, 680, 916, 1261, 1411, 1471, 1560
BssAI RCCGGY 2 cut(s) 300, 349
BssECI CCNNGG 3 cut(s) 152, 229, 804
BssMI GATC 3 cut(s) 89, 234, 392
BssNAI GTATAC 1 cut(s) 1232
BssNI GRCGYC 3 cut(s) 344, 458, 783
BssT1I CCWWGG 1 cut(s) 804
Bst1107I GTATAC 1 cut(s) 1232
Bst2UI CCWGG 1 cut(s) 1289
Bst4CI ACNGT 3 cut(s) 7, 759, 839
BstACI GRCGYC 3 cut(s) 344, 458, 783
BstBI TTCGAA 1 cut(s) 566
BstC8I GCNNGC 2 cut(s) 1040, 1418
BstDEI CTNAG 4 cut(s) 715, 1029, 1119, 1487
BstEII GGTNACC 1 cut(s) 1176
BstENI CCTNNNNNAGG 1 cut(s) 379
BstF5I GGATG 6 cut(s) 189, 637, 865, 966, 1379, 1483
BstKTI GATC 3 cut(s) 92, 237, 395
BstMAI GTCTC 3 cut(s) 951, 994, 1129
BstMBI GATC 3 cut(s) 89, 234, 392
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 8 cut(s) 62, 211, 514, 523, 532, 1120, 1163, 1293
BstNI CCWGG 1 cut(s) 1289
BstNSI RCATGY 2 cut(s) 490, 1420
BstPAI GACNNNNGTC 1 cut(s) 1460
BstPI GGTNACC 1 cut(s) 1176
BstSCI CCNGG 6 cut(s) 152, 161, 179, 228, 512, 1287
BstSFI CTRYAG 2 cut(s) 435, 1155
BstV1I GCAGC 2 cut(s) 291, 1141
BstV2I GAAGAC 3 cut(s) 15, 21, 272
BstX2I RGATCY 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 326
BstYI RGATCY 1 cut(s) 234
BstZ17I GTATAC 1 cut(s) 1232
BsuI GTATCC 3 cut(s) 930, 1337, 1386
BtrI CACGTC 1 cut(s) 276
BtsCI GGATG 6 cut(s) 189, 637, 865, 966, 1379, 1483
BtsI GCAGTG 1 cut(s) 1245
BtsIMutI CAGTG 3 cut(s) 1245, 1464, 1553
BveI ACCTGC 2 cut(s) 428, 1135
Cac8I GCNNGC 2 cut(s) 1040, 1418
CaiI CAGNNNCTG 2 cut(s) 385, 1494
Cfr10I RCCGGY 2 cut(s) 300, 349
Cfr13I GGNCC 7 cut(s) 515, 604, 678, 770, 801, 880, 1050
CseI GACGC 3 cut(s) 333, 447, 772
Csp6I GTAC 2 cut(s) 579, 831
CviAII CATG 9 cut(s) 487, 551, 924, 977, 1305, 1340, 1384, 1417, 1530
CviQI GTAC 2 cut(s) 579, 831
DdeI CTNAG 4 cut(s) 715, 1029, 1119, 1487
DpnI GATC 3 cut(s) 91, 236, 394
DpnII GATC 3 cut(s) 89, 234, 392
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 4 cut(s) 841, 1018, 1255, 1408
Eam1105I GACNNNNNGTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 804
Eco147I AGGCCT 2 cut(s) 81, 374
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 951
Eco47I GGWCC 5 cut(s) 604, 678, 801, 880, 1050
Eco57I CTGAAG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 238
Eco91I GGTNACC 1 cut(s) 1176
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 2 cut(s) 604, 770
EcoO65I GGTNACC 1 cut(s) 1176
EcoRII CCWGG 1 cut(s) 1287
EcoT14I CCWWGG 1 cut(s) 804
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 804
FaeI CATG 9 cut(s) 490, 554, 927, 980, 1308, 1343, 1387, 1420, 1533
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 4 cut(s) 459, 590, 877, 1572
FatI CATG 9 cut(s) 486, 550, 923, 976, 1304, 1339, 1383, 1416, 1529
FauI CCCGC 2 cut(s) 356, 1031
FblI GTMKAC 2 cut(s) 194, 1231
Fnu4HI GCNGC 5 cut(s) 203, 280, 456, 1155, 1192
FokI GGATG 6 cut(s) 196, 644, 872, 953, 1366, 1490
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 5 cut(s) 203, 280, 456, 1155, 1192
FspBI CTAG 1 cut(s) 941
GluI GCNGC 5 cut(s) 203, 280, 456, 1155, 1192
GsaI CCCAGC 2 cut(s) 480, 1476
GsuI CTGGAG 2 cut(s) 202, 1227
HapII CCGG 8 cut(s) 154, 162, 180, 229, 301, 350, 513, 961
HgaI GACGC 3 cut(s) 333, 447, 772
Hin1I GRCGYC 3 cut(s) 344, 458, 783
Hin1II CATG 9 cut(s) 490, 554, 927, 980, 1308, 1343, 1387, 1420, 1533
HincII GTYRAC 2 cut(s) 195, 1276
HindII GTYRAC 2 cut(s) 195, 1276
HindIII AAGCTT 1 cut(s) 1010
HinfI GANTC 9 cut(s) 137, 191, 196, 209, 419, 664, 865, 987, 1491
HpaII CCGG 8 cut(s) 154, 162, 180, 229, 301, 350, 513, 961
HphI GGTGA 5 cut(s) 132, 364, 760, 1170, 1434
Hpy166II GTNNAC 5 cut(s) 195, 673, 896, 1232, 1276
Hpy188III TCNNGA 9 cut(s) 238, 499, 629, 647, 948, 1206, 1240, 1369, 1495
Hpy8I GTNNAC 5 cut(s) 195, 673, 896, 1232, 1276
HpyAV CCTTC 5 cut(s) 349, 871, 980, 1205, 1518
HpyCH4III ACNGT 3 cut(s) 7, 759, 839
HpyCH4IV ACGT 1 cut(s) 275
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 211, 514, 523, 532, 1120, 1163, 1293
HpyF3I CTNAG 4 cut(s) 715, 1029, 1119, 1487
HpySE526I ACGT 1 cut(s) 275
Hsp92I GRCGYC 3 cut(s) 344, 458, 783
Hsp92II CATG 9 cut(s) 490, 554, 927, 980, 1308, 1343, 1387, 1420, 1533
Kzo9I GATC 3 cut(s) 89, 234, 392
LmnI GCTCC 5 cut(s) 70, 321, 397, 505, 640
Lsp1109I GCAGC 2 cut(s) 291, 1141
LweI GCATC 3 cut(s) 622, 743, 1011
MaeI CTAG 1 cut(s) 941
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 6 cut(s) 748, 761, 1176, 1440, 1462, 1551
MalI GATC 3 cut(s) 91, 236, 394
MbiI CCGCTC 1 cut(s) 1191
MboI GATC 3 cut(s) 89, 234, 392
MboII GAAGA 5 cut(s) 15, 21, 93, 277, 420
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 3 cut(s) 16, 168, 1149
MlsI TGGCCA 4 cut(s) 843, 1020, 1257, 1410
MluCI AATT 3 cut(s) 115, 825, 1359
MluNI TGGCCA 4 cut(s) 843, 1020, 1257, 1410
MlyI GAGTC 5 cut(s) 190, 200, 203, 859, 996
MmeI TCCRAC 3 cut(s) 682, 1077, 1547
Mox20I TGGCCA 4 cut(s) 843, 1020, 1257, 1410
Mph1103I ATGCAT 1 cut(s) 736
MscI TGGCCA 4 cut(s) 843, 1020, 1257, 1410
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 4 cut(s) 843, 1020, 1257, 1410
MspA1I CMGCKG 1 cut(s) 1154
MspI CCGG 8 cut(s) 154, 162, 180, 229, 301, 350, 513, 961
MspR9I CCNGG 6 cut(s) 154, 163, 181, 230, 514, 1289
MunI CAATTG 1 cut(s) 825
Mva1269I GAATGC 2 cut(s) 508, 736
MvaI CCWGG 1 cut(s) 1289
MwoI GCNNNNNNNGC 8 cut(s) 62, 211, 514, 523, 532, 1120, 1163, 1293
NciI CCSGG 5 cut(s) 154, 163, 181, 230, 514
NdeII GATC 3 cut(s) 89, 234, 392
NlaIII CATG 9 cut(s) 490, 554, 927, 980, 1308, 1343, 1387, 1420, 1533
NlaIV GGNNCC 5 cut(s) 317, 606, 712, 771, 1109
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 6 cut(s) 748, 761, 1176, 1440, 1462, 1551
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 2 cut(s) 490, 1420
NspV TTCGAA 1 cut(s) 566
PaeI GCATGC 1 cut(s) 1420
PaeR7I CTCGAG 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 428
PceI AGGCCT 2 cut(s) 81, 374
PciI ACATGT 1 cut(s) 486
PcsI WCGNNNNNNNCGW 5 cut(s) 245, 257, 260, 269, 366
PctI GAATGC 2 cut(s) 508, 736
PfeI GAWTC 4 cut(s) 137, 419, 664, 1491
PflMI CCANNNNNTGG 2 cut(s) 1173, 1363
PkrI GCNGC 5 cut(s) 204, 281, 457, 1156, 1193
PleI GAGTC 5 cut(s) 190, 199, 203, 859, 995
PpsI GAGTC 5 cut(s) 190, 199, 203, 859, 995
PpuMI RGGWCCY 1 cut(s) 604
PscI ACATGT 1 cut(s) 486
PshAI GACNNNNGTC 1 cut(s) 1460
Psp5II RGGWCCY 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 1287
PspEI GGTNACC 1 cut(s) 1176
PspFI CCCAGC 2 cut(s) 476, 1472
PspGI CCWGG 1 cut(s) 1287
PspN4I GGNNCC 5 cut(s) 317, 606, 712, 771, 1109
PspPI GGNCC 7 cut(s) 515, 604, 678, 770, 801, 880, 1050
PspPPI RGGWCCY 1 cut(s) 604
PsrI GAACNNNNNNTAC 2 cut(s) 359, 391
PstI CTGCAG 2 cut(s) 439, 1159
PstNI CAGNNNCTG 2 cut(s) 385, 1494
PsuI RGATCY 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 1154
RsaI GTAC 2 cut(s) 580, 832
RsaNI GTAC 2 cut(s) 579, 831
RseI CAYNNNNRTG 1 cut(s) 555
SalI GTCGAC 1 cut(s) 193
SatI GCNGC 5 cut(s) 203, 280, 456, 1155, 1192
Sau3AI GATC 3 cut(s) 89, 234, 392
Sau96I GGNCC 7 cut(s) 515, 604, 678, 770, 801, 880, 1050
SbfI CCTGCAGG 1 cut(s) 439
SchI GAGTC 5 cut(s) 190, 200, 203, 859, 996
ScrFI CCNGG 6 cut(s) 154, 163, 181, 230, 514, 1289
SdaI CCTGCAGG 1 cut(s) 439
SduI GDGCHC 3 cut(s) 16, 168, 1149
SfaNI GCATC 3 cut(s) 622, 743, 1011
SfcI CTRYAG 2 cut(s) 435, 1155
Sfr274I CTCGAG 1 cut(s) 238
SfuI TTCGAA 1 cut(s) 566
SgrAI CRCCGGYG 1 cut(s) 349
SinI GGWCC 5 cut(s) 604, 678, 801, 880, 1050
SlaI CTCGAG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 2 cut(s) 238, 931
SmoI CTYRAG 2 cut(s) 238, 931
SphI GCATGC 1 cut(s) 1420
Sse8387I CCTGCAGG 1 cut(s) 439
Sse9I AATT 3 cut(s) 115, 825, 1359
SseBI AGGCCT 2 cut(s) 81, 374
SsiI CCGC 7 cut(s) 143, 202, 363, 455, 493, 1038, 1191
SspMI CTAG 1 cut(s) 941
StuI AGGCCT 2 cut(s) 81, 374
StyD4I CCNGG 6 cut(s) 152, 161, 179, 228, 512, 1287
StyI CCWWGG 1 cut(s) 804
TaaI ACNGT 3 cut(s) 7, 759, 839
TaiI ACGT 1 cut(s) 278
TaqI TCGA 9 cut(s) 194, 207, 239, 263, 272, 311, 417, 539, 566
TasI AATT 3 cut(s) 115, 825, 1359
TatI WGTACW 1 cut(s) 578
TauI GCSGC 3 cut(s) 205, 458, 1194
TfiI GAWTC 4 cut(s) 137, 419, 664, 1491
TscAI CASTG 3 cut(s) 1252, 1471, 1560
TseFI GTSAC 6 cut(s) 748, 761, 1176, 1440, 1462, 1551
TseI GCWGC 2 cut(s) 279, 1154
Tsp45I GTSAC 6 cut(s) 748, 761, 1176, 1440, 1462, 1551
TspDTI ATGAA 3 cut(s) 280, 804, 1494
TspGWI ACGGA 2 cut(s) 285, 518
TspRI CASTG 3 cut(s) 1252, 1471, 1560
Van91I CCANNNNNTGG 2 cut(s) 1173, 1363
VpaK11BI GGWCC 5 cut(s) 604, 678, 801, 880, 1050
XagI CCTNNNNNAGG 1 cut(s) 379
XceI RCATGY 2 cut(s) 490, 1420
XhoI CTCGAG 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 194, 1231
XspI CTAG 1 cut(s) 941
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.