Rroxscaffold_4G00289160

Fizzy-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009046
Physical Location & Seq
Forward (+)
9849936 .. 9853061
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289160.1

Sequence Viewer

Length: 1344 bp
ATGGATTCACCGCAAACTCCCCGGAAAACCGGGCTCAACCTCCCAACCGGGATGTCCGAGTCGACTCTGAGACTCGACTCGCTCTCCAGCTCGTTCCGGGGGATCTCGAGCCTCTCGTCGTCGTCGCCGTCGAAGACCTCGACGTGCAGCGACAGGTTCATACCGTGCCGGTCGTCGTCGAGGCTCCACCAGTTTGGGCTGAACGACAAGGCGTCGCCGGTGAAGGACGGCGGGAACGAGGCCTACCAGAGGCTGTTGAGATCGGAGCTTTTCGGGTCTGATTTCGATTCTTTTTCTTCTCCTGCAGGTGGAGGAGGACAGGTGGCGTCGTCGCCGATTAGTCCCAGCAAGAACATGTTGCGGTTCAAGACGGAGCATTCCGGGCCAAACTCGCCGTATTCGCCGTCGATTTTGAGGCATGATAGTGGGTTTTCGAATGAGAGCTGTACTCCTCCCAAGCCTCCCAGAAAGGTCCCCAAGACACCCCATAAGGTTCTGGATGCTCCATCACTTCAAGATGACTTTTACTTGAATCTGGTGGACTGGTCCTCGCAAAATGTCCTTGCAGTTGGATTGGGAACCTCAGTTTATTTATGGAATGCATCAAACAGCAAGGTGACAAGACTGTGTGACTTGGGGCCTAATGATGGCGTCTGCTCTGTTCAATGGACCAAGGAGGGTTCGTATTTATCAATTGGTACAAACGGTGGCCAAGTTCAGGTTTGGGATGGGACTCAATGCAAGAAGGTCCGAACAATGAGTGGACATCAAACAAGAACTGGTGTTTTGGCATGGAACTCAAGGATACTAGCTTCAGGAAGCCGAGACCGGAACATCCTTCAACATGATATGCGAGTCTCAAATGACTGCATCAACAAGCTTGTTGGCCACAAGTCTGAGGTATGCGGGCTGAAATGGTCCAACGATGACAGGGAACTTGCTTCTGGTGGCAATGATAATCAGCTCTTGGTGTGGAACCAGCACTCTCAGCAACCTGCGTTGAGACTAACAGAGCACACAGCTGCAGTAAAGGCCATTGCTTGGTCACCCCACCAGAGCGGCCTTCTTGCTTCTGGAGGTGGAACTGCTGACAGATGTATACGCTTCTGGAACACTGCAAATGGCCACCAGTTGAACAGCGTTGACACGGGAAGCCAGGTTGCCACTCTAACTGGCCATAGCATGCGAGTTCTATACCTTGCAATGTCACCAGATGGTCAGACAATAGTCACTGGTGCTGGGGATGAAACTCTCAGATTCTGGAATGTGTTTCCTTCGGTAAAAACTCCGACACCATGCAAAGAAACAGGTCTTTGGTCACTGGGACGAACTCAAATCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914
Pfam Domains
Protein Families
WD40

Protein Analysis

447

Amino Acids

48.42

Weight (kDa)

9.14

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 166 - 390 4.1e-93 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 177 - 267 3.6e-07 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 181 - 279 7.6e-12 WDR19 first beta-propeller
Beta-prop_THOC3 PF25174 181 - 301 1.7e-09 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 184 - 349 1.4e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 187 - 284 1.3e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 189 - 288 7.6e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 191 - 298 2.1e-11 WDR5 beta-propeller domain
WDR55 PF24796 209 - 357 9.7e-06 WDR55
WD40_MABP1-WDR62_2nd PF24782 214 - 379 3e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 266 - 374 3.3e-08 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 266 - 346 2.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 266 - 380 6.8e-18 WDR5 beta-propeller domain
WD40_Prp19 PF24814 267 - 423 2.2e-16 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 287 - 423 3.1e-24 WDR3 first beta-propeller domain
WD40 PF00400 289 - 326 4.1e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 291 - 380 2.6e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 295 - 423 2.6e-20 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 296 - 356 1.9e-07 WDHD1 first WD40 domain
Beta-prop_EML PF23409 296 - 423 5.5e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 297 - 423 3.6e-23 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 301 - 430 2.4e-11 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 316 - 423 3.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 378 - 426 3.5e-06 G protein beta WD-40 repeat protein
WD40 PF00400 384 - 422 1.3e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 386 - 421 4.2e-10 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 2 cut(s) 296, 1003
AccB7I CCANNNNNTGG 1 cut(s) 1041
AccBSI CCGCTC 1 cut(s) 1059
AccI GTMKAC 2 cut(s) 62, 1099
AciI CCGC 5 cut(s) 11, 231, 361, 906, 1059
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 4 cut(s) 709, 886, 1123, 1174
AcuI CTGAAG 1 cut(s) 798
AcyI GRCGYC 3 cut(s) 212, 326, 651
AfaI GTAC 2 cut(s) 448, 700
AfiI CCNNNNNNNGG 5 cut(s) 249, 308, 647, 718, 1041
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 6 cut(s) 367, 515, 532, 665, 842, 1135
AhdI GACNNNNNGTC 1 cut(s) 211
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 1 cut(s) 1155
AluBI AGCT 7 cut(s) 90, 268, 444, 812, 880, 964, 1022
AluI AGCT 7 cut(s) 90, 268, 444, 812, 880, 964, 1022
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 4 cut(s) 64, 819, 862, 997
AlwI GGATC 1 cut(s) 110
AlwNI CAGNNNCTG 2 cut(s) 253, 1260
Ama87I CYCGRG 1 cut(s) 106
AoxI GGCC 9 cut(s) 240, 383, 638, 709, 886, 1032, 1060, 1123, 1174
ApeKI GCWGC 2 cut(s) 147, 1022
AspS9I GGNCC 7 cut(s) 383, 472, 546, 638, 669, 748, 918
AsuC2I CCSGG 5 cut(s) 22, 31, 49, 98, 382
AsuHPI GGTGA 4 cut(s) 232, 628, 1038, 1200
AsuII TTCGAA 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 5 cut(s) 472, 546, 669, 748, 918
BalI TGGCCA 4 cut(s) 711, 888, 1125, 1176
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 2 cut(s) 159, 1009
BccI CCATC 4 cut(s) 514, 641, 722, 1208
BceAI ACGGC 4 cut(s) 112, 244, 379, 388
BciT130I CCWGG 1 cut(s) 1157
BciVI GTATCC 1 cut(s) 798
BcnI CCSGG 5 cut(s) 22, 31, 49, 98, 382
BcoDI GTCTC 4 cut(s) 64, 819, 862, 997
BfaI CTAG 1 cut(s) 809
BfmI CTRYAG 2 cut(s) 303, 1023
BfuAI ACCTGC 2 cut(s) 296, 1003
BfuI GTATCC 1 cut(s) 798
BisI GCNGC 3 cut(s) 148, 1023, 1060
BlsI GCNGC 3 cut(s) 149, 1024, 1061
Bme1390I CCNGG 6 cut(s) 22, 31, 49, 98, 382, 1157
Bme18I GGWCC 5 cut(s) 472, 546, 669, 748, 918
BmeRI GACNNNNNGTC 1 cut(s) 211
BmeT110I CYCGRG 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 7 cut(s) 383, 472, 546, 638, 669, 748, 918
BmiI GGNNCC 5 cut(s) 185, 474, 580, 639, 977
BmrFI CCNGG 6 cut(s) 22, 31, 49, 98, 382, 1157
BmrI ACTGGG 1 cut(s) 1331
BmsI GCATC 3 cut(s) 490, 611, 879
BmuI ACTGGG 1 cut(s) 1331
BoxI GACNNNNGTC 1 cut(s) 1226
BpiI GAAGAC 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 433, 465
BpmI CTGGAG 2 cut(s) 70, 1095
Bpu14I TTCGAA 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 784
BpuMI CCSGG 5 cut(s) 22, 31, 49, 98, 382
BsaHI GRCGYC 3 cut(s) 212, 326, 651
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 3 cut(s) 20, 97, 672
BsaWI WCCGGW 1 cut(s) 828
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 306, 336, 668, 698
Bsc4I CCNNNNNNNGG 5 cut(s) 249, 308, 647, 718, 1041
Bse118I RCCGGY 2 cut(s) 168, 217
Bse1I ACTGG 7 cut(s) 190, 548, 784, 1129, 1177, 1237, 1326
Bse3DI GCAATG 3 cut(s) 958, 1035, 1209
BseBI CCWGG 1 cut(s) 1157
BseDI CCNNGG 3 cut(s) 20, 97, 672
BseGI GGATG 5 cut(s) 57, 505, 733, 834, 1249
BseLI CCNNNNNNNGG 5 cut(s) 249, 308, 647, 718, 1041
BseMI GCAATG 3 cut(s) 958, 1035, 1209
BseMII CTCAG 5 cut(s) 59, 597, 888, 1001, 1267
BseNI ACTGG 7 cut(s) 190, 548, 784, 1129, 1177, 1237, 1326
BseRI GAGGAG 2 cut(s) 327, 441
BseXI GCAGC 2 cut(s) 159, 1009
BseYI CCCAGC 2 cut(s) 344, 1238
BsgI GTGCAG 1 cut(s) 166
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 9 cut(s) 242, 385, 640, 711, 888, 1034, 1062, 1125, 1176
BsiEI CGRYCG 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 8 cut(s) 22, 30, 48, 97, 169, 218, 381, 829
BslFI GGGAC 4 cut(s) 327, 458, 745, 1338
BslI CCNNNNNNNGG 5 cut(s) 249, 308, 647, 718, 1041
BsmAI GTCTC 4 cut(s) 64, 819, 862, 997
BsmFI GGGAC 4 cut(s) 327, 458, 745, 1338
BsmI GAATGC 2 cut(s) 376, 604
BsnI GGCC 9 cut(s) 242, 385, 640, 711, 888, 1034, 1062, 1125, 1176
Bso31I GGTCTC 1 cut(s) 819
BsoBI CYCGRG 1 cut(s) 106
Bsp119I TTCGAA 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 36, 1017
Bsp143I GATC 2 cut(s) 102, 260
BspACI CCGC 5 cut(s) 11, 231, 361, 906, 1059
BspANI GGCC 9 cut(s) 242, 385, 640, 711, 888, 1034, 1062, 1125, 1176
BspCNI CTCAG 5 cut(s) 60, 596, 889, 1000, 1266
BspLI GGNNCC 5 cut(s) 185, 474, 580, 639, 977
BspMAI CTGCAG 2 cut(s) 307, 1027
BspMI ACCTGC 2 cut(s) 296, 1003
BspPI GGATC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 819
BsrBI CCGCTC 1 cut(s) 1059
BsrDI GCAATG 3 cut(s) 958, 1035, 1209
BsrFI RCCGGY 2 cut(s) 168, 217
BsrI ACTGG 7 cut(s) 190, 548, 784, 1129, 1177, 1237, 1326
BssAI RCCGGY 2 cut(s) 168, 217
BssECI CCNNGG 3 cut(s) 20, 97, 672
BssMI GATC 2 cut(s) 102, 260
BssNAI GTATAC 1 cut(s) 1100
BssNI GRCGYC 3 cut(s) 212, 326, 651
BssT1I CCWWGG 1 cut(s) 672
Bst1107I GTATAC 1 cut(s) 1100
Bst2UI CCWGG 1 cut(s) 1157
Bst4CI ACNGT 3 cut(s) 165, 627, 707
BstACI GRCGYC 3 cut(s) 212, 326, 651
BstBI TTCGAA 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 908, 1184
BstDEI CTNAG 5 cut(s) 68, 583, 897, 987, 1253
BstEII GGTNACC 1 cut(s) 1044
BstENI CCTNNNNNAGG 1 cut(s) 247
BstF5I GGATG 5 cut(s) 57, 505, 733, 834, 1249
BstKTI GATC 2 cut(s) 105, 263
BstMAI GTCTC 4 cut(s) 64, 819, 862, 997
BstMBI GATC 2 cut(s) 102, 260
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 5 cut(s) 382, 391, 400, 988, 1031
BstNI CCWGG 1 cut(s) 1157
BstNSI RCATGY 2 cut(s) 358, 1186
BstPAI GACNNNNGTC 1 cut(s) 1226
BstPI GGTNACC 1 cut(s) 1044
BstSCI CCNGG 6 cut(s) 20, 29, 47, 96, 380, 1155
BstSFI CTRYAG 2 cut(s) 303, 1023
BstV1I GCAGC 2 cut(s) 159, 1009
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 102
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 102
BstZ17I GTATAC 1 cut(s) 1100
BsuI GTATCC 1 cut(s) 798
BsuRI GGCC 9 cut(s) 242, 385, 640, 711, 888, 1034, 1062, 1125, 1176
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 5 cut(s) 57, 505, 733, 834, 1249
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 3 cut(s) 1113, 1230, 1319
BveI ACCTGC 2 cut(s) 296, 1003
Cac8I GCNNGC 2 cut(s) 908, 1184
CaiI CAGNNNCTG 2 cut(s) 253, 1260
Cfr10I RCCGGY 2 cut(s) 168, 217
Cfr13I GGNCC 7 cut(s) 383, 472, 546, 638, 669, 748, 918
CseI GACGC 3 cut(s) 201, 315, 640
Csp6I GTAC 2 cut(s) 447, 699
CviAII CATG 6 cut(s) 355, 419, 792, 845, 1183, 1296
CviQI GTAC 2 cut(s) 447, 699
DdeI CTNAG 5 cut(s) 68, 583, 897, 987, 1253
DpnI GATC 2 cut(s) 104, 262
DpnII GATC 2 cut(s) 102, 260
DriI GACNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 4 cut(s) 709, 886, 1123, 1174
Eam1105I GACNNNNNGTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 672
Eco147I AGGCCT 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 5 cut(s) 472, 546, 669, 748, 918
Eco57I CTGAAG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 106
Eco91I GGTNACC 1 cut(s) 1044
EcoNI CCTNNNNNAGG 1 cut(s) 247
EcoO109I RGGNCCY 2 cut(s) 472, 638
EcoO65I GGTNACC 1 cut(s) 1044
EcoRII CCWGG 1 cut(s) 1155
EcoT14I CCWWGG 1 cut(s) 672
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 672
FaeI CATG 6 cut(s) 358, 422, 795, 848, 1186, 1299
FaqI GGGAC 4 cut(s) 327, 458, 745, 1338
FatI CATG 6 cut(s) 354, 418, 791, 844, 1182, 1295
FauI CCCGC 2 cut(s) 224, 899
FblI GTMKAC 2 cut(s) 62, 1099
Fnu4HI GCNGC 3 cut(s) 148, 1023, 1060
FokI GGATG 5 cut(s) 64, 512, 740, 821, 1256
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 3 cut(s) 148, 1023, 1060
FspBI CTAG 1 cut(s) 809
GluI GCNGC 3 cut(s) 148, 1023, 1060
GsaI CCCAGC 2 cut(s) 348, 1242
GsuI CTGGAG 2 cut(s) 70, 1095
HaeIII GGCC 9 cut(s) 242, 385, 640, 711, 888, 1034, 1062, 1125, 1176
HapII CCGG 8 cut(s) 22, 30, 48, 97, 169, 218, 381, 829
HgaI GACGC 3 cut(s) 201, 315, 640
Hin1I GRCGYC 3 cut(s) 212, 326, 651
Hin1II CATG 6 cut(s) 358, 422, 795, 848, 1186, 1299
HincII GTYRAC 2 cut(s) 63, 1144
HindII GTYRAC 2 cut(s) 63, 1144
HindIII AAGCTT 1 cut(s) 878
HpaII CCGG 8 cut(s) 22, 30, 48, 97, 169, 218, 381, 829
HphI GGTGA 4 cut(s) 232, 628, 1038, 1200
Hpy166II GTNNAC 5 cut(s) 63, 541, 764, 1100, 1144
Hpy188III TCNNGA 8 cut(s) 106, 367, 497, 515, 816, 1074, 1108, 1261
Hpy8I GTNNAC 5 cut(s) 63, 541, 764, 1100, 1144
HpyAV CCTTC 5 cut(s) 217, 739, 848, 1073, 1284
HpyCH4III ACNGT 3 cut(s) 165, 627, 707
HpyCH4IV ACGT 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 5 cut(s) 382, 391, 400, 988, 1031
HpyF3I CTNAG 5 cut(s) 68, 583, 897, 987, 1253
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 3 cut(s) 212, 326, 651
Hsp92II CATG 6 cut(s) 358, 422, 795, 848, 1186, 1299
Kzo9I GATC 2 cut(s) 102, 260
LmnI GCTCC 4 cut(s) 189, 265, 373, 508
Lsp1109I GCAGC 2 cut(s) 159, 1009
LweI GCATC 3 cut(s) 490, 611, 879
MaeI CTAG 1 cut(s) 809
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 6 cut(s) 616, 629, 1044, 1206, 1228, 1317
MalI GATC 2 cut(s) 104, 262
MbiI CCGCTC 1 cut(s) 1059
MboI GATC 2 cut(s) 102, 260
MboII GAAGA 2 cut(s) 145, 288
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 2 cut(s) 36, 1017
MlsI TGGCCA 4 cut(s) 711, 888, 1125, 1176
MluCI AATT 1 cut(s) 693
MluNI TGGCCA 4 cut(s) 711, 888, 1125, 1176
MlyI GAGTC 6 cut(s) 58, 66, 68, 71, 727, 864
MmeI TCCRAC 3 cut(s) 550, 945, 1313
Mox20I TGGCCA 4 cut(s) 711, 888, 1125, 1176
Mph1103I ATGCAT 1 cut(s) 604
MscI TGGCCA 4 cut(s) 711, 888, 1125, 1176
MslI CAYNNNNRTG 1 cut(s) 423
Msp20I TGGCCA 4 cut(s) 711, 888, 1125, 1176
MspA1I CMGCKG 1 cut(s) 1022
MspI CCGG 8 cut(s) 22, 30, 48, 97, 169, 218, 381, 829
MspR9I CCNGG 6 cut(s) 22, 31, 49, 98, 382, 1157
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 2 cut(s) 376, 604
MvaI CCWGG 1 cut(s) 1157
MwoI GCNNNNNNNGC 5 cut(s) 382, 391, 400, 988, 1031
NciI CCSGG 5 cut(s) 22, 31, 49, 98, 382
NdeII GATC 2 cut(s) 102, 260
NlaIII CATG 6 cut(s) 358, 422, 795, 848, 1186, 1299
NlaIV GGNNCC 5 cut(s) 185, 474, 580, 639, 977
NmeAIII GCCGAG 1 cut(s) 848
NmuCI GTSAC 6 cut(s) 616, 629, 1044, 1206, 1228, 1317
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 358, 1186
NspV TTCGAA 1 cut(s) 434
PaeI GCATGC 1 cut(s) 1186
PaeR7I CTCGAG 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 296
PceI AGGCCT 1 cut(s) 242
PciI ACATGT 1 cut(s) 354
PcsI WCGNNNNNNNCGW 5 cut(s) 113, 125, 128, 137, 234
PctI GAATGC 2 cut(s) 376, 604
PfeI GAWTC 4 cut(s) 5, 287, 532, 1257
PflMI CCANNNNNTGG 1 cut(s) 1041
PkrI GCNGC 3 cut(s) 149, 1024, 1061
PleI GAGTC 6 cut(s) 58, 66, 67, 71, 727, 863
PpsI GAGTC 6 cut(s) 58, 66, 67, 71, 727, 863
PpuMI RGGWCCY 1 cut(s) 472
PscI ACATGT 1 cut(s) 354
PshAI GACNNNNGTC 1 cut(s) 1226
Psp5II RGGWCCY 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 1155
PspEI GGTNACC 1 cut(s) 1044
PspFI CCCAGC 2 cut(s) 344, 1238
PspGI CCWGG 1 cut(s) 1155
PspN4I GGNNCC 5 cut(s) 185, 474, 580, 639, 977
PspPI GGNCC 7 cut(s) 383, 472, 546, 638, 669, 748, 918
PspPPI RGGWCCY 1 cut(s) 472
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PstI CTGCAG 2 cut(s) 307, 1027
PstNI CAGNNNCTG 2 cut(s) 253, 1260
PsuI RGATCY 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 1022
RsaI GTAC 2 cut(s) 448, 700
RsaNI GTAC 2 cut(s) 447, 699
RseI CAYNNNNRTG 1 cut(s) 423
SalI GTCGAC 1 cut(s) 61
SatI GCNGC 3 cut(s) 148, 1023, 1060
Sau3AI GATC 2 cut(s) 102, 260
Sau96I GGNCC 7 cut(s) 383, 472, 546, 638, 669, 748, 918
SbfI CCTGCAGG 1 cut(s) 307
SchI GAGTC 6 cut(s) 58, 66, 68, 71, 727, 864
ScrFI CCNGG 6 cut(s) 22, 31, 49, 98, 382, 1157
SdaI CCTGCAGG 1 cut(s) 307
SduI GDGCHC 2 cut(s) 36, 1017
SfaNI GCATC 3 cut(s) 490, 611, 879
SfcI CTRYAG 2 cut(s) 303, 1023
Sfr274I CTCGAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 434
SgrAI CRCCGGYG 1 cut(s) 217
SinI GGWCC 5 cut(s) 472, 546, 669, 748, 918
SlaI CTCGAG 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 2 cut(s) 106, 799
SmoI CTYRAG 2 cut(s) 106, 799
SphI GCATGC 1 cut(s) 1186
Sse8387I CCTGCAGG 1 cut(s) 307
Sse9I AATT 1 cut(s) 693
SseBI AGGCCT 1 cut(s) 242
SsiI CCGC 5 cut(s) 11, 231, 361, 906, 1059
SspMI CTAG 1 cut(s) 809
StuI AGGCCT 1 cut(s) 242
StyD4I CCNGG 6 cut(s) 20, 29, 47, 96, 380, 1155
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 3 cut(s) 165, 627, 707
TaiI ACGT 1 cut(s) 146
TaqI TCGA 9 cut(s) 62, 75, 107, 131, 140, 179, 285, 407, 434
TasI AATT 1 cut(s) 693
TatI WGTACW 1 cut(s) 446
TauI GCSGC 1 cut(s) 1062
TfiI GAWTC 4 cut(s) 5, 287, 532, 1257
TscAI CASTG 3 cut(s) 1120, 1237, 1326
TseFI GTSAC 6 cut(s) 616, 629, 1044, 1206, 1228, 1317
TseI GCWGC 2 cut(s) 147, 1022
Tsp45I GTSAC 6 cut(s) 616, 629, 1044, 1206, 1228, 1317
TspDTI ATGAA 2 cut(s) 148, 1260
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 3 cut(s) 1120, 1237, 1326
Van91I CCANNNNNTGG 1 cut(s) 1041
VpaK11BI GGWCC 5 cut(s) 472, 546, 669, 748, 918
XagI CCTNNNNNAGG 1 cut(s) 247
XceI RCATGY 2 cut(s) 358, 1186
XhoI CTCGAG 1 cut(s) 106
XmiI GTMKAC 2 cut(s) 62, 1099
XspI CTAG 1 cut(s) 809
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.