MD07G1200700.v1.1

Fizzy-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009046
Physical Location & Seq
Reverse (-)
27856261 .. 27859401
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1200700.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGATATCGTCACATTCTTCAGACCCAGCAACCCAGCACCTCCTCCTCCAAGCAACCAAGAACCGCATCCGAGAAAACCCGGCAATGGAGTCACCTCAAACGAGGAAATACGGGCTTAACCTCCCCACCGGAATGTCCGAGACACTGCTCCGCCTTGATTCCGTACCGGTTTCCTTTCGGGGGGTTTCGAGCCTCTCAGCGTCCCCCGGTTCTTGTCGGGCGATTTCGAACCTGTCCTCGCCGTCCAAGTCCTCGACTTGCAGCGACAGATTCATCCCCTGCAGATCCTCTTCGCGGCTTCACACATTCGGGCTCAACGAGAAGGCCTCGCCGGTGAAAGAAGGCGGCAACGAGGCGTATGCCAGATTGTTGAAATCGGAGCTTTTCGGGTCTGATTTCGGGTCTTTTTCTCCTGCAGGTGGTGGCAGCGGTGGTGCGGCGTCCCCAATGAGTCCCAGCAAGAACATGCTGCGGTTCAAGACGGAGAACTCTGGACCCAACTCGCCGTATTCGCCTTCCCTTCTGAGAAATGACAGTGGGTTTTCCAGCGAGACAACGCCTCCCAAGCCTCCTCGCAAGGTCCCCAAGACACCCCATAAGGTTCTGGATGCTCCATCACTACAAGATGACTTTTACTTGAACCTTGTGGACTGGTCTTCCCAAAATGTCCTTGCAGTTGGACTGGGGACCAGTGTTTATCTTTGGAGTGCAACAAACAGCAAGGTGACAAAACTGTGCGACTTGGGGCCTAATGACGGCGTGTGCTCTGTCCAGTGGACACGGGAGGGGTCATACTTGTCAATTGGCACAAGTCTTGGTCGAGTTCAGGTTTGGGATGGGACGCAGTGCAAGAGGGTTCGAACGATGGGCGGGCATCAAACAAGGACTGGAGTTTTGGCATGGAACTCACGCATATTAGCTTCAGGAAGCCGAGACCGGAACATACGTCAACACGATCTTCGAGTCCCAGATAATTACATCAGCAAACTTGTTGGCCACAAGTCTGAGGTATGTGGGCTGAAATGGTCCAATGATGACAGGGAACTTGCATCCGGTGGCAATGATAATCAGCTCTTGGTCTGGAACCAGCATTCTCAGCAACCGGCTTTGAGGCTGACAGAGCACACAGCTGCTGTCAAGGCCATATCTTGGTCACCTCACCAGAGTGGCCTTCTCGCATCTGGAGGAGGAACTGCTGACAGATGTATTCGGTTCTGGAACACTACAAACGGCCACCAATTGAACAGTATCGACACAGGAAGCCAGGTCTGCAATCTTGCATGGAGTAAGAATGTGAATGAGATAGTCAGCACTCATGGGTACTCCCAGAATCAAATTATGGTTTGGAAGTATCCATCCATGGGAAAGGTTGCCACTTTAACTGGCCATAGCATGCGAGTTCTGTACCTTGCAATATCACCAGATGGTCAGACAATAGTGACGGGCGCTGGGGATGAGACTCTAAGATTCTGGAACGTCTTCCCATCTGTGAAGACACATACATCAGTGAAGGATACAGGTCTTTGGTCACTAGGACGAACTCAGATTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914
Pfam Domains
Protein Families
WD40

Protein Analysis

518

Amino Acids

56.16

Weight (kDa)

9.55

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 202 - 491 2e-127 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 217 - 320 2.8e-08 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 217 - 315 6.9e-12 WDR19 first beta-propeller
Beta-prop_EML_2 PF23414 220 - 389 5e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 223 - 320 1.4e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 227 - 321 1.5e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 230 - 322 1.5e-06 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 248 - 492 9.5e-15 MABP1/WDR62 second WD40 domain
WDR55 PF24796 254 - 496 7.8e-10 WDR55
Beta-prop_WDR5 PF25175 303 - 419 1e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 304 - 493 5.8e-20 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 305 - 428 1.8e-08 TEP-1 second beta-propeller
WD40 PF00400 327 - 362 2.6e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 328 - 437 9.4e-17 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 329 - 499 8.9e-12 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 329 - 417 4.7e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 330 - 439 2.2e-06 EIF3I
WD40_WDHD1_1st PF24817 333 - 392 7.2e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 333 - 438 1.2e-21 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 340 - 494 4.3e-06 WDR19 first beta-propeller
WD40_MABP1-WDR62_1st PF24780 372 - 499 3.7e-06 MABP1/WDR62 first WD40 domain
Beta-prop_EML PF23409 373 - 494 1.8e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 374 - 494 4.8e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 375 - 492 2.1e-09 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 399 - 501 1.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 421 - 495 1.2e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 423 - 494 5.5e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 442 - 494 2.8e-08 THOC3 beta-propeller domain
WD40 PF00400 457 - 492 2.5e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 407
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccII CGCG 1 cut(s) 295
AciI CCGC 8 cut(s) 64, 151, 295, 345, 429, 437, 472, 870
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 3 cut(s) 994, 1231, 1384
AcuI CTGAAG 1 cut(s) 906
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 3 cut(s) 165, 1322, 1406
AfiI CCNNNNNNNGG 7 cut(s) 85, 180, 294, 419, 755, 1149, 1361
AgeI ACCGGT 1 cut(s) 166
AgsI TTSAA 4 cut(s) 373, 478, 640, 1243
AjnI CCWGG 1 cut(s) 1263
AleI CACNNNNGTG 1 cut(s) 1164
AluBI AGCT 4 cut(s) 382, 920, 1072, 1130
AluI AGCT 4 cut(s) 382, 920, 1072, 1130
Alw21I GWGCWC 2 cut(s) 767, 1125
Alw26I GTCTC 4 cut(s) 134, 545, 927, 1451
AlwI GGATC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 1133
AoxI GGCC 7 cut(s) 324, 746, 994, 1140, 1168, 1231, 1384
ApeKI GCWGC 4 cut(s) 261, 426, 469, 1130
ArsI GACNNNNNNTTYG 2 cut(s) 877, 909
AsiGI ACCGGT 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 1478
AspLEI GCGC 1 cut(s) 1448
AspS9I GGNCC 5 cut(s) 494, 580, 687, 746, 1026
AsuC2I CCSGG 2 cut(s) 80, 207
AsuHPI GGTGA 6 cut(s) 84, 346, 736, 1146, 1151, 1410
AsuII TTCGAA 2 cut(s) 227, 859
AvaII GGWCC 4 cut(s) 494, 580, 687, 1026
BalI TGGCCA 2 cut(s) 996, 1386
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 3 cut(s) 648, 1471, 1499
Bbv12I GWGCWC 2 cut(s) 767, 1125
BbvI GCAGC 4 cut(s) 273, 438, 456, 1117
BccI CCATC 6 cut(s) 622, 830, 859, 1363, 1418, 1492
BceAI ACGGC 4 cut(s) 226, 490, 772, 1246
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 1 cut(s) 1265
BciVI GTATCC 2 cut(s) 1362, 1507
BcnI CCSGG 2 cut(s) 80, 207
BcoDI GTCTC 4 cut(s) 134, 545, 927, 1451
BfaI CTAG 1 cut(s) 1532
BfmI CTRYAG 2 cut(s) 280, 414
BfoI RGCGCY 1 cut(s) 1449
BfuAI ACCTGC 1 cut(s) 407
BfuI GTATCC 2 cut(s) 1362, 1507
BisI GCNGC 7 cut(s) 262, 296, 346, 427, 438, 470, 1131
BlsI GCNGC 7 cut(s) 263, 297, 347, 428, 439, 471, 1132
Bme1390I CCNGG 3 cut(s) 80, 207, 1265
Bme18I GGWCC 4 cut(s) 494, 580, 687, 1026
BmgT120I GGNCC 5 cut(s) 494, 580, 687, 746, 1026
BmiI GGNNCC 5 cut(s) 496, 582, 688, 747, 1085
BmrFI CCNGG 3 cut(s) 80, 207, 1265
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 5 cut(s) 75, 598, 883, 1058, 1187
BmuI ACTGGG 1 cut(s) 692
BpiI GAAGAC 3 cut(s) 648, 1471, 1499
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 2 cut(s) 909, 1203
Bpu14I TTCGAA 2 cut(s) 227, 859
BpuMI CCSGG 2 cut(s) 80, 207
BsaHI GRCGYC 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 927
BsaJI CCNNGG 2 cut(s) 205, 1359
BsaWI WCCGGW 4 cut(s) 128, 166, 936, 1052
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 7 cut(s) 85, 180, 294, 419, 755, 1149, 1361
Bse118I RCCGGY 3 cut(s) 166, 331, 1102
Bse1I ACTGG 6 cut(s) 656, 687, 690, 772, 892, 1387
Bse3DI GCAATG 2 cut(s) 90, 1066
BseBI CCWGG 1 cut(s) 1265
BseDI CCNNGG 2 cut(s) 205, 1359
BseGI GGATG 7 cut(s) 66, 273, 613, 841, 1049, 1355, 1459
BseLI CCNNNNNNNGG 7 cut(s) 85, 180, 294, 419, 755, 1149, 1361
BseMI GCAATG 2 cut(s) 90, 1066
BseMII CTCAG 4 cut(s) 210, 515, 996, 1109
BseNI ACTGG 6 cut(s) 656, 687, 690, 772, 892, 1387
BseRI GAGGAG 4 cut(s) 32, 35, 561, 1200
BseXI GCAGC 4 cut(s) 273, 438, 456, 1117
BseYI CCCAGC 4 cut(s) 25, 33, 455, 1448
Bsh1236I CGCG 1 cut(s) 295
BshFI GGCC 7 cut(s) 326, 748, 996, 1142, 1170, 1233, 1386
BshTI ACCGGT 1 cut(s) 166
BsiHKAI GWGCWC 2 cut(s) 767, 1125
BsiSI CCGG 8 cut(s) 80, 129, 167, 207, 332, 937, 1053, 1103
BslFI GGGAC 7 cut(s) 187, 427, 438, 566, 700, 853, 950
BslI CCNNNNNNNGG 7 cut(s) 85, 180, 294, 419, 755, 1149, 1361
BsmAI GTCTC 4 cut(s) 134, 545, 927, 1451
BsmFI GGGAC 7 cut(s) 187, 427, 438, 566, 700, 853, 950
BsmI GAATGC 1 cut(s) 1090
BsnI GGCC 7 cut(s) 326, 748, 996, 1142, 1170, 1233, 1386
Bso31I GGTCTC 1 cut(s) 927
Bsp119I TTCGAA 2 cut(s) 227, 859
Bsp1286I GDGCHC 3 cut(s) 315, 767, 1125
Bsp143I GATC 2 cut(s) 284, 955
Bsp19I CCATGG 1 cut(s) 1359
BspACI CCGC 8 cut(s) 64, 151, 295, 345, 429, 437, 472, 870
BspANI GGCC 7 cut(s) 326, 748, 996, 1142, 1170, 1233, 1386
BspCNI CTCAG 4 cut(s) 209, 516, 997, 1108
BspFNI CGCG 1 cut(s) 295
BspLI GGNNCC 5 cut(s) 496, 582, 688, 747, 1085
BspMAI CTGCAG 2 cut(s) 284, 418
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 1 cut(s) 279
BspT104I TTCGAA 2 cut(s) 227, 859
BspTNI GGTCTC 1 cut(s) 927
BsrDI GCAATG 2 cut(s) 90, 1066
BsrFI RCCGGY 3 cut(s) 166, 331, 1102
BsrI ACTGG 6 cut(s) 656, 687, 690, 772, 892, 1387
BssAI RCCGGY 3 cut(s) 166, 331, 1102
BssECI CCNNGG 2 cut(s) 205, 1359
BssMI GATC 2 cut(s) 284, 955
BssNI GRCGYC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 1359
Bst2UI CCWGG 1 cut(s) 1265
Bst4CI ACNGT 3 cut(s) 536, 735, 1247
Bst6I CTCTTC 1 cut(s) 295
BstACI GRCGYC 1 cut(s) 440
BstBI TTCGAA 2 cut(s) 227, 859
BstC8I GCNNGC 2 cut(s) 872, 1394
BstDEI CTNAG 6 cut(s) 196, 524, 1005, 1095, 1463, 1542
BstDSI CCRYGG 1 cut(s) 1359
BstEII GGTNACC 1 cut(s) 1152
BstF5I GGATG 7 cut(s) 66, 273, 613, 841, 1049, 1355, 1459
BstFNI CGCG 1 cut(s) 295
BstH2I RGCGCY 1 cut(s) 1449
BstHHI GCGC 1 cut(s) 1448
BstKTI GATC 2 cut(s) 287, 958
BstMAI GTCTC 4 cut(s) 134, 545, 927, 1451
BstMBI GATC 2 cut(s) 284, 955
BstMWI GCNNNNNNNGC 6 cut(s) 511, 565, 1096, 1120, 1139, 1269
BstNI CCWGG 1 cut(s) 1265
BstNSI RCATGY 2 cut(s) 469, 1396
BstPI GGTNACC 1 cut(s) 1152
BstSCI CCNGG 3 cut(s) 78, 205, 1263
BstSFI CTRYAG 2 cut(s) 280, 414
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 4 cut(s) 273, 438, 456, 1117
BstV2I GAAGAC 3 cut(s) 648, 1471, 1499
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuI GTATCC 2 cut(s) 1362, 1507
BsuRI GGCC 7 cut(s) 326, 748, 996, 1142, 1170, 1233, 1386
BtgI CCRYGG 1 cut(s) 1359
BtsCI GGATG 7 cut(s) 66, 273, 613, 841, 1049, 1355, 1459
BtsI GCAGTG 2 cut(s) 143, 851
BtsIMutI CAGTG 6 cut(s) 143, 541, 697, 779, 851, 1512
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 2 cut(s) 872, 1394
CaiI CAGNNNCTG 1 cut(s) 1133
CfoI GCGC 1 cut(s) 1448
Cfr10I RCCGGY 3 cut(s) 166, 331, 1102
Cfr13I GGNCC 5 cut(s) 494, 580, 687, 746, 1026
CseI GACGC 3 cut(s) 189, 429, 850
Csp6I GTAC 3 cut(s) 164, 1321, 1405
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 6 cut(s) 466, 900, 1281, 1316, 1360, 1393
CviQI GTAC 3 cut(s) 164, 1321, 1405
DdeI CTNAG 6 cut(s) 196, 524, 1005, 1095, 1463, 1542
DpnI GATC 2 cut(s) 286, 957
DpnII GATC 2 cut(s) 284, 955
EaeI YGGCCR 3 cut(s) 994, 1231, 1384
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
EciI GGCGGA 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 1359
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 927
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 4 cut(s) 494, 580, 687, 1026
Eco57I CTGAAG 1 cut(s) 906
Eco91I GGTNACC 1 cut(s) 1152
EcoO109I RGGNCCY 2 cut(s) 580, 746
EcoO65I GGTNACC 1 cut(s) 1152
EcoRII CCWGG 1 cut(s) 1263
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 1359
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 1359
FaeI CATG 6 cut(s) 469, 903, 1284, 1319, 1363, 1396
FaqI GGGAC 7 cut(s) 187, 427, 438, 566, 700, 853, 950
FatI CATG 6 cut(s) 465, 899, 1280, 1315, 1359, 1392
FauI CCCGC 1 cut(s) 863
Fnu4HI GCNGC 7 cut(s) 262, 296, 346, 427, 438, 470, 1131
FokI GGATG 7 cut(s) 53, 260, 620, 848, 1036, 1342, 1466
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 7 cut(s) 262, 296, 346, 427, 438, 470, 1131
FspBI CTAG 1 cut(s) 1532
GlaI GCGC 1 cut(s) 1447
GluI GCNGC 7 cut(s) 262, 296, 346, 427, 438, 470, 1131
GsaI CCCAGC 4 cut(s) 29, 37, 459, 1452
GsuI CTGGAG 2 cut(s) 909, 1203
HaeII RGCGCY 1 cut(s) 1449
HaeIII GGCC 7 cut(s) 326, 748, 996, 1142, 1170, 1233, 1386
HapII CCGG 8 cut(s) 80, 129, 167, 207, 332, 937, 1053, 1103
HgaI GACGC 3 cut(s) 189, 429, 850
HhaI GCGC 1 cut(s) 1448
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 6 cut(s) 469, 903, 1284, 1319, 1363, 1396
Hin6I GCGC 1 cut(s) 1446
HinP1I GCGC 1 cut(s) 1446
HincII GTYRAC 1 cut(s) 950
HindII GTYRAC 1 cut(s) 950
HinfI GANTC 9 cut(s) 89, 158, 270, 451, 963, 1330, 1459, 1467, 1546
HpaII CCGG 8 cut(s) 80, 129, 167, 207, 332, 937, 1053, 1103
HphI GGTGA 6 cut(s) 84, 346, 736, 1146, 1151, 1410
Hpy166II GTNNAC 3 cut(s) 649, 777, 950
Hpy188I TCNGA 9 cut(s) 22, 71, 139, 379, 394, 525, 1006, 1431, 1545
Hpy188III TCNNGA 8 cut(s) 478, 492, 605, 924, 1081, 1182, 1216, 1471
Hpy8I GTNNAC 3 cut(s) 649, 777, 950
HpyAV CCTTC 6 cut(s) 316, 335, 525, 530, 1181, 1504
HpyCH4III ACNGT 3 cut(s) 536, 735, 1247
HpyCH4IV ACGT 2 cut(s) 946, 1476
HpyF10VI GCNNNNNNNGC 6 cut(s) 511, 565, 1096, 1120, 1139, 1269
HpyF3I CTNAG 6 cut(s) 196, 524, 1005, 1095, 1463, 1542
HpySE526I ACGT 2 cut(s) 946, 1476
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 6 cut(s) 469, 903, 1284, 1319, 1363, 1396
HspAI GCGC 1 cut(s) 1446
Kzo9I GATC 2 cut(s) 284, 955
LmnI GCTCC 3 cut(s) 153, 379, 616
Lsp1109I GCAGC 4 cut(s) 273, 438, 456, 1117
LweI GCATC 5 cut(s) 75, 598, 883, 1058, 1187
MaeI CTAG 1 cut(s) 1532
MaeII ACGT 2 cut(s) 946, 1476
MaeIII GTNAC 6 cut(s) 9, 90, 724, 1152, 1438, 1527
MalI GATC 2 cut(s) 286, 957
MboI GATC 2 cut(s) 284, 955
MboII GAAGA 6 cut(s) 9, 282, 648, 950, 1471, 1504
MfeI CAATTG 2 cut(s) 801, 1238
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 3 cut(s) 315, 767, 1125
MlsI TGGCCA 2 cut(s) 996, 1386
MluCI AATT 4 cut(s) 801, 973, 1238, 1335
MluNI TGGCCA 2 cut(s) 996, 1386
MlyI GAGTC 4 cut(s) 98, 460, 972, 1453
MmeI TCCRAC 1 cut(s) 658
Mox20I TGGCCA 2 cut(s) 996, 1386
MroXI GAANNNNTTC 1 cut(s) 1478
MscI TGGCCA 2 cut(s) 996, 1386
MseI TTAA 2 cut(s) 117, 1379
MslI CAYNNNNRTG 2 cut(s) 131, 1164
Msp20I TGGCCA 2 cut(s) 996, 1386
MspA1I CMGCKG 2 cut(s) 429, 1130
MspI CCGG 8 cut(s) 80, 129, 167, 207, 332, 937, 1053, 1103
MspR9I CCNGG 3 cut(s) 80, 207, 1265
MunI CAATTG 2 cut(s) 801, 1238
Mva1269I GAATGC 1 cut(s) 1090
MvaI CCWGG 1 cut(s) 1265
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 6 cut(s) 511, 565, 1096, 1120, 1139, 1269
NciI CCSGG 2 cut(s) 80, 207
NcoI CCATGG 1 cut(s) 1359
NdeII GATC 2 cut(s) 284, 955
NlaIII CATG 6 cut(s) 469, 903, 1284, 1319, 1363, 1396
NlaIV GGNNCC 5 cut(s) 496, 582, 688, 747, 1085
NmeAIII GCCGAG 1 cut(s) 956
NmuCI GTSAC 6 cut(s) 9, 90, 724, 1152, 1438, 1527
NspI RCATGY 2 cut(s) 469, 1396
NspV TTCGAA 2 cut(s) 227, 859
OliI CACNNNNGTG 1 cut(s) 1164
PaeI GCATGC 1 cut(s) 1396
PaqCI CACCTGC 1 cut(s) 407
PceI AGGCCT 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 1090
PdmI GAANNNNTTC 1 cut(s) 1478
PfeI GAWTC 5 cut(s) 158, 270, 1330, 1467, 1546
PflMI CCANNNNNTGG 1 cut(s) 1149
PinAI ACCGGT 1 cut(s) 166
PkrI GCNGC 7 cut(s) 263, 297, 347, 428, 439, 471, 1132
PleI GAGTC 4 cut(s) 97, 459, 971, 1453
PpsI GAGTC 4 cut(s) 97, 459, 971, 1453
PpuMI RGGWCCY 1 cut(s) 580
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 1263
PspEI GGTNACC 1 cut(s) 1152
PspFI CCCAGC 4 cut(s) 25, 33, 455, 1448
PspGI CCWGG 1 cut(s) 1263
PspN4I GGNNCC 5 cut(s) 496, 582, 688, 747, 1085
PspPI GGNCC 5 cut(s) 494, 580, 687, 746, 1026
PspPPI RGGWCCY 1 cut(s) 580
PstI CTGCAG 2 cut(s) 284, 418
PstNI CAGNNNCTG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 1130
RsaI GTAC 3 cut(s) 165, 1322, 1406
RsaNI GTAC 3 cut(s) 164, 1321, 1405
RseI CAYNNNNRTG 2 cut(s) 131, 1164
SaqAI TTAA 2 cut(s) 117, 1379
SatI GCNGC 7 cut(s) 262, 296, 346, 427, 438, 470, 1131
Sau3AI GATC 2 cut(s) 284, 955
Sau96I GGNCC 5 cut(s) 494, 580, 687, 746, 1026
SbfI CCTGCAGG 1 cut(s) 418
SchI GAGTC 4 cut(s) 98, 460, 972, 1453
ScrFI CCNGG 3 cut(s) 80, 207, 1265
SdaI CCTGCAGG 1 cut(s) 418
SduI GDGCHC 3 cut(s) 315, 767, 1125
SfaNI GCATC 5 cut(s) 75, 598, 883, 1058, 1187
SfcI CTRYAG 2 cut(s) 280, 414
SfuI TTCGAA 2 cut(s) 227, 859
SgrAI CRCCGGYG 1 cut(s) 331
SinI GGWCC 4 cut(s) 494, 580, 687, 1026
SmiMI CAYNNNNRTG 2 cut(s) 131, 1164
SphI GCATGC 1 cut(s) 1396
Sse8387I CCTGCAGG 1 cut(s) 418
Sse9I AATT 4 cut(s) 801, 973, 1238, 1335
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 8 cut(s) 64, 151, 295, 345, 429, 437, 472, 870
SspMI CTAG 1 cut(s) 1532
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 78, 205, 1263
StyI CCWWGG 1 cut(s) 1359
TaaI ACNGT 3 cut(s) 536, 735, 1247
TaiI ACGT 2 cut(s) 949, 1479
TaqI TCGA 8 cut(s) 188, 227, 254, 820, 859, 961, 1251, 1549
TasI AATT 4 cut(s) 801, 973, 1238, 1335
TauI GCSGC 3 cut(s) 298, 348, 440
TfiI GAWTC 5 cut(s) 158, 270, 1330, 1467, 1546
Tru1I TTAA 2 cut(s) 117, 1379
Tru9I TTAA 2 cut(s) 117, 1379
TscAI CASTG 6 cut(s) 150, 541, 697, 779, 851, 1512
TseFI GTSAC 6 cut(s) 9, 90, 724, 1152, 1438, 1527
TseI GCWGC 4 cut(s) 261, 426, 469, 1130
Tsp45I GTSAC 6 cut(s) 9, 90, 724, 1152, 1438, 1527
TspDTI ATGAA 1 cut(s) 262
TspGWI ACGGA 2 cut(s) 151, 497
TspRI CASTG 6 cut(s) 150, 541, 697, 779, 851, 1512
Van91I CCANNNNNTGG 1 cut(s) 1149
VpaK11BI GGWCC 4 cut(s) 494, 580, 687, 1026
XceI RCATGY 2 cut(s) 469, 1396
XmnI GAANNNNTTC 1 cut(s) 1478
XspI CTAG 1 cut(s) 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.