MD01G1167900.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013923
Physical Location & Seq
Reverse (-)
27235517 .. 27239553
4037 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1167900.v1.1.491

Sequence Viewer

Length: 2859 bp
ATGCGACCCTTTTCAGGCATGAAACCGCTGCTCCTCCTATTCGCCGTCGTCGTTTTGTCTCCGGTTCTCGCGAGATCTCTGAACCCGTCTCTGAACGACGACGTGTTGGGGCTGATAGTGTTCAAGGCGAATATCCAAGATCCGAAGGGAAAGTTAGCGTCTTGGAGCGAAGACGACGACAGTGCTTGCAGTTGGGTCGGGGTAAAATGCCACCCGAGATCCAACCGGGTCATCGAGCTCAACCTCGACGACTTCTCGCTTTCGGGTCACATCGGGCGCGGCCTTCTGCAACTGCAAGCGCTCCGGAAGCTCTCGCTTTCGAAGAATAATCTCACCGGAAGCTTAACGCCTAACATTGCTCACATCGACAACCTCCGAGTCCTCGATTTGAGTGAGAACAGTTTCTCCGGTGATGTTCCAGAAGAGTTTTTCCGGCAATGTGGGTCACTGAGAATGATTTCTCTGGCGAAAAACAAGTTTTCCGGGAAAATTCCGGAGAGTTTGGGCTCGTGTCCGAGTCTAGCTTCTGTTAACTTGTCGTTGAACCAGTTTTCGGGTTCGGTTCCGGCTGGGGTTTGGTCCTTGAGTGGGATTAGATCACTGGATTTGTCTGATAACTTGTTGGAGGGTGGAATTCCAAAGGGGGTTGAAGGATTGAATAATTTGCGAGGAATTAATTTGGCCAGGAATCGGTTTACTGGGCAAGTTCCAGATGGAATTGGAAACTGGTTGCTCTTGAGGTCCATTGACCTGAGCGAGAATTCGTTCTCGGGTAACCTCCCTCAGACAATGCAGAAACTTGGCCTGTGCAGCTATCTGAATTTGCACCAGAACACGTTTTCCGGTGAGGTTCCAGAGTGGATTGGAGAGGTGAAGAGCCTTGAGACTTTGGACCTTTCTAGCAACCAATTTTCGGGTGAAGTTCCAAGTTCAATAGGCAATCTTGAGTCTTTGAAAGTATTGAATTTTTCTGCAAATGGGTTCACTGGTAGCTTGCCAAAGTCTCTGTCTTACTGCACAAACCTTATGGCTTTAGATTTTAGCAAGAATTCGATGGCGGGTGAGCTTCCTGTGTGGATATTCGCGGCAGGTACAGAGGAAGTTTCACTTTCAGAGAAAAAACTAAGTGGAAGCAAGAACATCCCTCTATCTTCATCAGCTGGAAATGCACTAAAGAATCTCCAAGTCTTGGATTTATCCCTTAATCATTTTTCCGGTGAAATTACGTCTGACATTGGGGCTTTAAGCAGCTTGCACGTTTTGAACCTCTCTGGTAACTCTCTTGCTGGTCCTATTCCTGTGGCCATTGGAGACCTGAAGGTCCTGGACAATCTCGACTTGAGCGAAAATCGGCTCAATGGAACCATTCCAACTTCAATCGAGAATTGCTCTTCTCTAACCACTTTAATTGTATCACAGAACAAATTGACTGGTCCAATACCTGCAGCAATGTCCAAACTTACCAACTTACAAAATGTGGACTTGTCCTTCAATAGCCTCACGGGAGGCCTGCCGAAGCATCTAGCCAATCTTCCCAACCTTCTTTCCTTCAACATTTCCCACAACAACCTCCAGGGTGAACTACCTGCGGGTGCCTTTTTCAACACCATCTCCCCTTCCTCCGTCTCTGGAAATCCATCTCTCTGTGGTTCTGCAGTTAATAAGTCTTGCCCAGGAGTCCTTCCCAAACCCATTGTCCTCAATCCCAATTCCTCTTCTGATTCCAGCACAGGAGAAATCTCATCAAATCTTGGTCATCGAAGAATCTTACTCAGTATTTCTTCGCTTATTGCCATTGCTGCAGCCGCTGTCATTGTCATTGGTGTAATTGCCATTACCGTGCTTAATCTCCGTGTCCGGTCTTCCTCTTCCACAACTCAACCGGCGGCAGCCCTCGCATTTTCAGGTGGGGATGACTTCAGCCATTCCCCTACAACAGATGGAAACTCTGGTAAGCTTGTCATGTTCTCAGGTGAGCCTGACTTCAGCACCAGCGCACATGCACTGCTCAACAAGGACTGTGAGCTTGGGCGAGGCGGGTTTGGAGCAGTCTATCGGACTGTTCTTCGAGATGGACGACCAGTTGCAATCAAGAAGCTCACTGTTTCTAGTCTTGTGAAGTCTCAAGGAGAATTTGAAAGGGAAGTGAAGAAATTGGGGAAAGTGAGGCACGATAATCTTGTGGAGATCGAAGGCTATTACTGGACTCCATCATTACAGCTCATCATATACGAATATGTTGCCGGTGGTAGTTTATACAAGCATCTTCATGATGGAGCAGGTGGAAACTTCCTGTCGTGGAATGACCGGTTCAACATAATTCTTGGAACTGCGAAAAGTTTGGCTCACTTGCACCAGATGAACATAATTCACTACAATATAAAGTCCAGCAATGTCCTGATTGGCAGCTCAGGTGAACCCAAAGTGGGAGACTTTGGCCTGGCTAGGTTGTTGCCAATGCTTGACCGCTATGTTCTGAGCAGCAAGATTCAGAGCGCCCTTGGCTACATGGCACCAGAGTTTGCTTGCAAAACGGTAAAGATAACCGAGAAATGTGATGTGTACGGGTTTGGGATTTTGGTTCTAGAGATTGTCACCGGGAAAAGACCTGTCGAGTACATGGAAGATGATGTTGTGGTGCTTTGTGACATGGTGAGAGAAGCACTGGAAGAAGGCAGGGTAGAAGAATGCGTTGATGCTCGGCTCCAAGGAATTTTCCCAGCAGAAGAGGCTATACCTGTGATGAAACTAGGCTTGATATGCACGTCACAAGTGCCATCAAACAGACCAGACATGGGGGAGGTCGTCAACATTTTGGAGATGATCCGATGTCCTTCCGAAGGCCAAGAGGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

953

Amino Acids

101.95

Weight (kDa)

5.8

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 70 1.6e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 124 - 184 1.9e-07 Leucine rich repeat
LRR_14 PF23598 192 - 301 5.9e-07 Leucine-rich repeat region
LRR_14 PF23598 391 - 523 2.1e-11 Leucine-rich repeat region
LRR_8 PF13855 442 - 500 1.3e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 672 - 937 6.4e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 673 - 877 1.4e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2270
AasI GACNNNNNNGTC 1 cut(s) 1319
Acc36I ACCTGC 4 cut(s) 1079, 1450, 1594, 2270
AccB1I GGYRCC 2 cut(s) 1592, 2512
AccB7I CCANNNNNTGG 1 cut(s) 1189
AccII CGCG 3 cut(s) 71, 279, 1085
AccIII TCCGGA 2 cut(s) 303, 493
AciI CCGC 9 cut(s) 26, 279, 1058, 1085, 1589, 1806, 1886, 2037, 2467
AclWI GGATC 3 cut(s) 134, 213, 2818
AcoI YGGCCR 2 cut(s) 681, 1302
AcsI RAATTY 8 cut(s) 489, 633, 760, 820, 964, 1048, 2132, 2712
AcuI CTGAAG 3 cut(s) 1337, 1903, 1969
AfaI GTAC 3 cut(s) 1093, 2564, 2618
AfeI AGCGCT 1 cut(s) 300
AfiI CCNNNNNNNGG 9 cut(s) 14, 553, 588, 690, 913, 1189, 2426, 2795, 2840
AflIII ACRYGT 2 cut(s) 102, 834
AgeI ACCGGT 1 cut(s) 2307
AjiI CACGTC 2 cut(s) 103, 2766
AjnI CCWGG 5 cut(s) 683, 1323, 1572, 1672, 2439
AloI GAACNNNNNNTCC 4 cut(s) 389, 421, 824, 856
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 8 cut(s) 63, 93, 878, 1008, 1305, 1630, 2127, 2424
AlwI GGATC 3 cut(s) 134, 213, 2818
AlwNI CAGNNNCTG 1 cut(s) 1808
Ama87I CYCGRG 2 cut(s) 214, 769
Aor13HI TCCGGA 2 cut(s) 303, 493
Aor51HI AGCGCT 1 cut(s) 300
AoxI GGCC 7 cut(s) 280, 681, 802, 1302, 1507, 2437, 2842
ApeKI GCWGC 9 cut(s) 28, 810, 1248, 1445, 1799, 1802, 1889, 2406, 2481
ApoI RAATTY 8 cut(s) 489, 633, 760, 820, 964, 1048, 2132, 2712
ArsI GACNNNNNNTTYG 4 cut(s) 992, 1024, 1680, 1712
AseI ATTAAT 1 cut(s) 675
AsiGI ACCGGT 1 cut(s) 2307
Asp700I GAANNNNTTC 3 cut(s) 401, 457, 764
AspLEI GCGC 4 cut(s) 279, 301, 1997, 2498
AspS9I GGNCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
AsuC2I CCSGG 3 cut(s) 227, 484, 2599
AsuII TTCGAA 1 cut(s) 320
AvaI CYCGRG 2 cut(s) 214, 769
AvaII GGWCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
BalI TGGCCA 2 cut(s) 683, 1304
BanI GGYRCC 2 cut(s) 1592, 2512
BanII GRGCYC 2 cut(s) 240, 509
BarI GAAGNNNNNNTAC 2 cut(s) 2718, 2750
BauI CACGAG 1 cut(s) 508
BbsI GAAGAC 2 cut(s) 177, 1854
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 9 cut(s) 15, 822, 1260, 1457, 1786, 1814, 1901, 2418, 2493
BccI CCATC 9 cut(s) 707, 1048, 1616, 1645, 1934, 2066, 2218, 2267, 2785
BceAI ACGGC 1 cut(s) 29
BcgI CGANNNNNNTGC 6 cut(s) 164, 178, 198, 212, 2222, 2256
BciT130I CCWGG 5 cut(s) 685, 1325, 1574, 1674, 2441
BcnI CCSGG 3 cut(s) 227, 484, 2599
BcoDI GTCTC 8 cut(s) 63, 93, 878, 1008, 1305, 1630, 2127, 2424
BfaI CTAG 7 cut(s) 521, 900, 1523, 2109, 2445, 2585, 2750
BfmI CTRYAG 3 cut(s) 1443, 1653, 1800
BfoI RGCGCY 2 cut(s) 302, 2499
BfuAI ACCTGC 4 cut(s) 1079, 1450, 1594, 2270
BglII AGATCT 1 cut(s) 74
Bme1390I CCNGG 8 cut(s) 227, 484, 685, 1325, 1574, 1674, 2441, 2599
Bme18I GGWCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
BmeT110I CYCGRG 2 cut(s) 214, 769
BmgBI CACGTC 2 cut(s) 103, 2766
BmgT120I GGNCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
BmiI GGNNCC 6 cut(s) 564, 852, 1363, 1594, 2514, 2705
BmrFI CCNGG 8 cut(s) 227, 484, 685, 1325, 1574, 1674, 2441, 2599
BmrI ACTGGG 1 cut(s) 708
BmsI GCATC 3 cut(s) 1528, 2272, 2686
BmuI ACTGGG 1 cut(s) 708
BpiI GAAGAC 2 cut(s) 177, 1854
BplI GAGNNNNNCTC 2 cut(s) 1373, 1405
BpmI CTGGAG 1 cut(s) 1556
Bpu10I CCTNAGC 2 cut(s) 752, 2410
Bpu14I TTCGAA 1 cut(s) 320
BpuEI CTTGAG 6 cut(s) 604, 757, 902, 965, 1360, 2109
BpuMI CCSGG 3 cut(s) 227, 484, 2599
BsaI GGTCTC 1 cut(s) 1305
BsaJI CCNNGG 4 cut(s) 1573, 1672, 2500, 2707
BsaWI WCCGGW 9 cut(s) 61, 303, 335, 407, 493, 842, 1214, 1857, 2307
BsaXI ACNNNNNCTCC 6 cut(s) 15, 45, 389, 419, 1595, 1625
Bsc4I CCNNNNNNNGG 9 cut(s) 14, 553, 588, 690, 913, 1189, 2426, 2795, 2840
Bse118I RCCGGY 3 cut(s) 1882, 2243, 2307
Bse1I ACTGG 9 cut(s) 547, 606, 703, 731, 991, 1435, 2081, 2207, 2670
Bse3DI GCAATG 5 cut(s) 354, 443, 1455, 1794, 2398
BseAI TCCGGA 2 cut(s) 303, 493
BseBI CCWGG 5 cut(s) 685, 1325, 1574, 1674, 2441
BseDI CCNNGG 4 cut(s) 1573, 1672, 2500, 2707
BseGI GGATG 2 cut(s) 1140, 1918
BseLI CCNNNNNNNGG 9 cut(s) 14, 553, 588, 690, 913, 1189, 2426, 2795, 2840
BseMI GCAATG 5 cut(s) 354, 443, 1455, 1794, 2398
BseMII CTCAG 7 cut(s) 440, 743, 797, 1786, 1983, 2424, 2468
BseNI ACTGG 9 cut(s) 547, 606, 703, 731, 991, 1435, 2081, 2207, 2670
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 9 cut(s) 15, 822, 1260, 1457, 1786, 1814, 1901, 2418, 2493
BseYI CCCAGC 2 cut(s) 569, 2719
BsgI GTGCAG 2 cut(s) 829, 1000
Bsh1236I CGCG 3 cut(s) 71, 279, 1085
BshFI GGCC 7 cut(s) 282, 683, 804, 1304, 1509, 2439, 2844
BshNI GGYRCC 2 cut(s) 1592, 2512
BshTI ACCGGT 1 cut(s) 2307
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 2 cut(s) 214, 769
BslI CCNNNNNNNGG 9 cut(s) 14, 553, 588, 690, 913, 1189, 2426, 2795, 2840
BsmAI GTCTC 8 cut(s) 63, 93, 878, 1008, 1305, 1630, 2127, 2424
BsmBI CGTCTC 2 cut(s) 93, 1630
BsmI GAATGC 1 cut(s) 2693
BsnI GGCC 7 cut(s) 282, 683, 804, 1304, 1509, 2439, 2844
Bso31I GGTCTC 1 cut(s) 1305
BsoBI CYCGRG 2 cut(s) 214, 769
Bsp119I TTCGAA 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 240, 509
Bsp13I TCCGGA 2 cut(s) 303, 493
Bsp143I GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
Bsp68I TCGCGA 1 cut(s) 71
BspACI CCGC 9 cut(s) 26, 279, 1058, 1085, 1589, 1806, 1886, 2037, 2467
BspANI GGCC 7 cut(s) 282, 683, 804, 1304, 1509, 2439, 2844
BspCNI CTCAG 7 cut(s) 441, 744, 796, 1785, 1982, 2423, 2469
BspEI TCCGGA 2 cut(s) 303, 493
BspFNI CGCG 3 cut(s) 71, 279, 1085
BspHI TCATGA 1 cut(s) 2269
BspLI GGNNCC 6 cut(s) 564, 852, 1363, 1594, 2514, 2705
BspMAI CTGCAG 3 cut(s) 1447, 1657, 1804
BspMI ACCTGC 4 cut(s) 1079, 1450, 1594, 2270
BspPI GGATC 3 cut(s) 134, 213, 2818
BspQI GCTCTTC 2 cut(s) 869, 1396
BspT104I TTCGAA 1 cut(s) 320
BspT107I GGYRCC 2 cut(s) 1592, 2512
BspTNI GGTCTC 1 cut(s) 1305
BsrDI GCAATG 5 cut(s) 354, 443, 1455, 1794, 2398
BsrFI RCCGGY 3 cut(s) 1882, 2243, 2307
BsrI ACTGG 9 cut(s) 547, 606, 703, 731, 991, 1435, 2081, 2207, 2670
BssAI RCCGGY 3 cut(s) 1882, 2243, 2307
BssECI CCNNGG 4 cut(s) 1573, 1672, 2500, 2707
BssMI GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
BssSI CACGAG 1 cut(s) 508
BssT1I CCWWGG 2 cut(s) 2500, 2707
Bst2BI CACGAG 1 cut(s) 508
Bst2UI CCWGG 5 cut(s) 685, 1325, 1574, 1674, 2441
Bst4CI ACNGT 7 cut(s) 182, 401, 1840, 2021, 2062, 2104, 2536
Bst6I CTCTTC 6 cut(s) 417, 869, 1396, 1720, 1873, 2721
BstBI TTCGAA 1 cut(s) 320
BstC8I GCNNGC 6 cut(s) 187, 297, 995, 1253, 1511, 2527
BstDEI CTNAG 8 cut(s) 449, 752, 783, 1124, 1772, 1969, 2410, 2477
BstEII GGTNACC 1 cut(s) 773
BstENI CCTNNNNNAGG 1 cut(s) 2838
BstF5I GGATG 2 cut(s) 1140, 1918
BstFNI CGCG 3 cut(s) 71, 279, 1085
BstH2I RGCGCY 2 cut(s) 302, 2499
BstHHI GCGC 4 cut(s) 279, 301, 1997, 2498
BstKTI GATC 6 cut(s) 77, 142, 221, 599, 2190, 2826
BstMAI GTCTC 8 cut(s) 63, 93, 878, 1008, 1305, 1630, 2127, 2424
BstMBI GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
BstMWI GCNNNNNNNGC 9 cut(s) 307, 810, 1166, 1799, 1805, 1895, 2502, 2729, 2760
BstNI CCWGG 5 cut(s) 685, 1325, 1574, 1674, 2441
BstNSI RCATGY 1 cut(s) 2003
BstPI GGTNACC 1 cut(s) 773
BstSCI CCNGG 8 cut(s) 225, 482, 683, 1323, 1572, 1672, 2439, 2597
BstSFI CTRYAG 3 cut(s) 1443, 1653, 1800
BstUI CGCG 3 cut(s) 71, 279, 1085
BstV1I GCAGC 9 cut(s) 15, 822, 1260, 1457, 1786, 1814, 1901, 2418, 2493
BstV2I GAAGAC 2 cut(s) 177, 1854
BstX2I RGATCY 3 cut(s) 74, 139, 218
BstYI RGATCY 3 cut(s) 74, 139, 218
BsuRI GGCC 7 cut(s) 282, 683, 804, 1304, 1509, 2439, 2844
BtrI CACGTC 2 cut(s) 103, 2766
BtsCI GGATG 2 cut(s) 1140, 1918
BtsI GCAGTG 1 cut(s) 2003
BtsIMutI CAGTG 7 cut(s) 187, 446, 599, 984, 2003, 2100, 2663
BtuMI TCGCGA 1 cut(s) 71
BveI ACCTGC 4 cut(s) 1079, 1450, 1594, 2270
Cac8I GCNNGC 6 cut(s) 187, 297, 995, 1253, 1511, 2527
CaiI CAGNNNCTG 1 cut(s) 1808
CciI TCATGA 1 cut(s) 2269
CfoI GCGC 4 cut(s) 279, 301, 1997, 2498
Cfr10I RCCGGY 3 cut(s) 1882, 2243, 2307
Cfr13I GGNCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
CseI GACGC 1 cut(s) 147
Csp6I GTAC 3 cut(s) 1092, 2563, 2617
CspAI ACCGGT 1 cut(s) 2307
CspCI CAANNNNNGTGG 2 cut(s) 1390, 1425
CviAII CATG 8 cut(s) 19, 1963, 2000, 2270, 2509, 2620, 2650, 2794
CviQI GTAC 3 cut(s) 1092, 2563, 2617
DdeI CTNAG 8 cut(s) 449, 752, 783, 1124, 1772, 1969, 2410, 2477
DpnI GATC 6 cut(s) 76, 141, 220, 598, 2189, 2825
DpnII GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
DrdI GACNNNNNNGTC 1 cut(s) 1319
DseDI GACNNNNNNGTC 1 cut(s) 1319
EaeI YGGCCR 2 cut(s) 681, 1302
Eam1104I CTCTTC 6 cut(s) 417, 869, 1396, 1720, 1873, 2721
EarI CTCTTC 6 cut(s) 417, 869, 1396, 1720, 1873, 2721
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 2500, 2707
Eco147I AGGCCT 1 cut(s) 1509
Eco24I GRGCYC 2 cut(s) 240, 509
Eco31I GGTCTC 1 cut(s) 1305
Eco47I GGWCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
Eco47III AGCGCT 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 3 cut(s) 1337, 1903, 1969
Eco88I CYCGRG 2 cut(s) 214, 769
Eco91I GGTNACC 1 cut(s) 773
EcoICRI GAGCTC 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 2838
EcoO109I RGGNCCY 1 cut(s) 1321
EcoO65I GGTNACC 1 cut(s) 773
EcoRI GAATTC 3 cut(s) 633, 760, 1048
EcoRII CCWGG 5 cut(s) 683, 1323, 1572, 1672, 2439
EcoT14I CCWWGG 2 cut(s) 2500, 2707
EcoT38I GRGCYC 2 cut(s) 240, 509
ErhI CCWWGG 2 cut(s) 2500, 2707
Esp3I CGTCTC 2 cut(s) 93, 1630
FaeI CATG 8 cut(s) 22, 1966, 2003, 2273, 2512, 2623, 2653, 2797
FalI AAGNNNNNCTT 2 cut(s) 1092, 1124
FatI CATG 8 cut(s) 18, 1962, 1999, 2269, 2508, 2619, 2649, 2793
FauI CCCGC 3 cut(s) 1051, 1582, 2030
FokI GGATG 2 cut(s) 1127, 1925
FriOI GRGCYC 2 cut(s) 240, 509
FspBI CTAG 7 cut(s) 521, 900, 1523, 2109, 2445, 2585, 2750
GlaI GCGC 4 cut(s) 278, 300, 1996, 2497
GsaI CCCAGC 2 cut(s) 573, 2723
GsuI CTGGAG 1 cut(s) 1556
HaeII RGCGCY 2 cut(s) 302, 2499
HaeIII GGCC 7 cut(s) 282, 683, 804, 1304, 1509, 2439, 2844
HgaI GACGC 1 cut(s) 147
HhaI GCGC 4 cut(s) 279, 301, 1997, 2498
Hin1II CATG 8 cut(s) 22, 1966, 2003, 2273, 2512, 2623, 2653, 2797
Hin6I GCGC 4 cut(s) 277, 299, 1995, 2496
HinP1I GCGC 4 cut(s) 277, 299, 1995, 2496
HincII GTYRAC 2 cut(s) 532, 2809
HindII GTYRAC 2 cut(s) 532, 2809
HindIII AAGCTT 2 cut(s) 340, 1955
HpaI GTTAAC 1 cut(s) 532
Hpy166II GTNNAC 8 cut(s) 532, 696, 984, 1480, 1580, 2417, 2563, 2809
Hpy8I GTNNAC 8 cut(s) 532, 696, 984, 1480, 1580, 2417, 2563, 2809
Hpy99I CGWCG 6 cut(s) 50, 53, 101, 104, 179, 251
HpyCH4III ACNGT 7 cut(s) 182, 401, 1840, 2021, 2062, 2104, 2536
HpyCH4IV ACGT 5 cut(s) 102, 836, 1226, 1257, 2765
HpyF10VI GCNNNNNNNGC 9 cut(s) 307, 810, 1166, 1799, 1805, 1895, 2502, 2729, 2760
HpyF3I CTNAG 8 cut(s) 449, 752, 783, 1124, 1772, 1969, 2410, 2477
HpySE526I ACGT 5 cut(s) 102, 836, 1226, 1257, 2765
Hsp92II CATG 8 cut(s) 22, 1966, 2003, 2273, 2512, 2623, 2653, 2797
HspAI GCGC 4 cut(s) 277, 299, 1995, 2496
Kpn2I TCCGGA 2 cut(s) 303, 493
KspAI GTTAAC 1 cut(s) 532
Kzo9I GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
LguI GCTCTTC 2 cut(s) 869, 1396
LmnI GCTCC 6 cut(s) 36, 165, 306, 2045, 2276, 2709
Lsp1109I GCAGC 9 cut(s) 15, 822, 1260, 1457, 1786, 1814, 1901, 2418, 2493
LweI GCATC 3 cut(s) 1528, 2272, 2686
MaeI CTAG 7 cut(s) 521, 900, 1523, 2109, 2445, 2585, 2750
MaeII ACGT 5 cut(s) 102, 836, 1226, 1257, 2765
MaeIII GTNAC 7 cut(s) 266, 444, 773, 1274, 2593, 2645, 2766
MalI GATC 6 cut(s) 76, 141, 220, 598, 2189, 2825
MboI GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
MflI RGATCY 3 cut(s) 74, 139, 218
MhlI GDGCHC 2 cut(s) 240, 509
MlsI TGGCCA 2 cut(s) 683, 1304
MluNI TGGCCA 2 cut(s) 683, 1304
MlyI GAGTC 5 cut(s) 387, 526, 956, 1686, 2200
MmeI TCCRAC 3 cut(s) 246, 603, 1394
Mox20I TGGCCA 2 cut(s) 683, 1304
MroI TCCGGA 2 cut(s) 303, 493
MroXI GAANNNNTTC 3 cut(s) 401, 457, 764
MscI TGGCCA 2 cut(s) 683, 1304
MseI TTAA 8 cut(s) 344, 531, 675, 1203, 1244, 1406, 1659, 1845
MslI CAYNNNNRTG 2 cut(s) 1838, 2268
Msp20I TGGCCA 2 cut(s) 683, 1304
MspA1I CMGCKG 3 cut(s) 28, 1160, 1808
MspR9I CCNGG 8 cut(s) 227, 484, 685, 1325, 1574, 1674, 2441, 2599
Mva1269I GAATGC 1 cut(s) 2693
MvaI CCWGG 5 cut(s) 685, 1325, 1574, 1674, 2441
MvnI CGCG 3 cut(s) 71, 279, 1085
MwoI GCNNNNNNNGC 9 cut(s) 307, 810, 1166, 1799, 1805, 1895, 2502, 2729, 2760
NciI CCSGG 3 cut(s) 227, 484, 2599
NdeII GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
NlaIII CATG 8 cut(s) 22, 1966, 2003, 2273, 2512, 2623, 2653, 2797
NlaIV GGNNCC 6 cut(s) 564, 852, 1363, 1594, 2514, 2705
NmeAIII GCCGAG 1 cut(s) 2680
NmuCI GTSAC 5 cut(s) 266, 444, 2593, 2645, 2766
NruI TCGCGA 1 cut(s) 71
NspI RCATGY 1 cut(s) 2003
NspV TTCGAA 1 cut(s) 320
PagI TCATGA 1 cut(s) 2269
PaqCI CACCTGC 1 cut(s) 2270
PceI AGGCCT 1 cut(s) 1509
PciSI GCTCTTC 2 cut(s) 869, 1396
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 1341, 2074
PctI GAATGC 1 cut(s) 2693
PdmI GAANNNNTTC 3 cut(s) 401, 457, 764
PfeI GAWTC 5 cut(s) 688, 1177, 1721, 1764, 2488
PflMI CCANNNNNTGG 1 cut(s) 1189
PfoI TCCNGGA 2 cut(s) 482, 1323
PinAI ACCGGT 1 cut(s) 2307
PleI GAGTC 5 cut(s) 386, 525, 955, 1685, 2200
PpsI GAGTC 5 cut(s) 386, 525, 955, 1685, 2200
PpuMI RGGWCCY 1 cut(s) 1321
PshBI ATTAAT 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 1321
Psp6I CCWGG 5 cut(s) 683, 1323, 1572, 1672, 2439
PspEI GGTNACC 1 cut(s) 773
PspFI CCCAGC 2 cut(s) 569, 2719
PspGI CCWGG 5 cut(s) 683, 1323, 1572, 1672, 2439
PspN4I GGNNCC 6 cut(s) 564, 852, 1363, 1594, 2514, 2705
PspPI GGNCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
PspPPI RGGWCCY 1 cut(s) 1321
PstI CTGCAG 3 cut(s) 1447, 1657, 1804
PstNI CAGNNNCTG 1 cut(s) 1808
PsuI RGATCY 3 cut(s) 74, 139, 218
PvuII CAGCTG 1 cut(s) 1160
RruI TCGCGA 1 cut(s) 71
RsaI GTAC 3 cut(s) 1093, 2564, 2618
RsaNI GTAC 3 cut(s) 1092, 2563, 2617
RseI CAYNNNNRTG 2 cut(s) 1838, 2268
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 2 cut(s) 869, 1396
SaqAI TTAA 8 cut(s) 344, 531, 675, 1203, 1244, 1406, 1659, 1845
Sau3AI GATC 6 cut(s) 74, 139, 218, 596, 2187, 2823
Sau96I GGNCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
SchI GAGTC 5 cut(s) 387, 526, 956, 1686, 2200
ScrFI CCNGG 8 cut(s) 227, 484, 685, 1325, 1574, 1674, 2441, 2599
SduI GDGCHC 2 cut(s) 240, 509
SfaNI GCATC 3 cut(s) 1528, 2272, 2686
SfcI CTRYAG 3 cut(s) 1443, 1653, 1800
SfuI TTCGAA 1 cut(s) 320
SinI GGWCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
SmiMI CAYNNNNRTG 2 cut(s) 1838, 2268
SmlI CTYRAG 6 cut(s) 583, 736, 881, 944, 1339, 2124
SmoI CTYRAG 6 cut(s) 583, 736, 881, 944, 1339, 2124
SseBI AGGCCT 1 cut(s) 1509
SsiI CCGC 9 cut(s) 26, 279, 1058, 1085, 1589, 1806, 1886, 2037, 2467
SspMI CTAG 7 cut(s) 521, 900, 1523, 2109, 2445, 2585, 2750
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 1 cut(s) 1509
StyD4I CCNGG 8 cut(s) 225, 482, 683, 1323, 1572, 1672, 2439, 2597
StyI CCWWGG 2 cut(s) 2500, 2707
TaaI ACNGT 7 cut(s) 182, 401, 1840, 2021, 2062, 2104, 2536
TaiI ACGT 5 cut(s) 105, 839, 1229, 1260, 2768
TatI WGTACW 1 cut(s) 2616
TauI GCSGC 4 cut(s) 282, 1088, 1808, 1889
TfiI GAWTC 5 cut(s) 688, 1177, 1721, 1764, 2488
Tru1I TTAA 8 cut(s) 344, 531, 675, 1203, 1244, 1406, 1659, 1845
Tru9I TTAA 8 cut(s) 344, 531, 675, 1203, 1244, 1406, 1659, 1845
TscAI CASTG 7 cut(s) 187, 453, 606, 991, 2010, 2107, 2670
TseFI GTSAC 5 cut(s) 266, 444, 2593, 2645, 2766
TseI GCWGC 9 cut(s) 28, 810, 1248, 1445, 1799, 1802, 1889, 2406, 2481
Tsp45I GTSAC 5 cut(s) 266, 444, 2593, 2645, 2766
TspDTI ATGAA 5 cut(s) 35, 1143, 2258, 2375, 2759
TspGWI ACGGA 2 cut(s) 1612, 1841
TspRI CASTG 7 cut(s) 187, 453, 606, 991, 2010, 2107, 2670
Van91I CCANNNNNTGG 1 cut(s) 1189
VpaK11BI GGWCC 6 cut(s) 579, 741, 892, 1289, 1321, 1433
VspI ATTAAT 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 2838
XapI RAATTY 8 cut(s) 489, 633, 760, 820, 964, 1048, 2132, 2712
XbaI TCTAGA 1 cut(s) 2584
XceI RCATGY 1 cut(s) 2003
XmnI GAANNNNTTC 3 cut(s) 401, 457, 764
XspI CTAG 7 cut(s) 521, 900, 1523, 2109, 2445, 2585, 2750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.