MD07G1233900.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013923
Physical Location & Seq
Reverse (-)
30745738 .. 30749881
4144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1233900.v1.1.491

Sequence Viewer

Length: 2922 bp
ATGCGACCGTTTTCAAGCATGAAACCGCTGCTCCTCCTCTTCGCCGTCGTCGTTTTGGCTCCGGTTCTCGCGAGATCTCTGAACCCGTCTCTAAACGACGACGTGTTGGGTCTGATAGTCTTTAAGGCCGATATCCAAGATCCGAAGGGGAAGCTAGCGTCGTGGAGCGAAGTCGACAACAGTCCTTGCAATTGGGTCGGGGTGAAATGCAACCCGAGATCCAACCGGGTCATCGAGCTCAGCCTCGACGACTTCTCGCTTTCGGGTCACATCGGCCGAGGCCTTCTGCAATTGCAAGCTCTCCGGAAGCTCTCGCTTTCGAAGAACAATCTCACCGGAAGCTTAACCCCTAACTTTACTCACATTGACAACCTCCGAGTCCTTGATTTGAGCGAGAACAGCTTCTCCGGTGGCGTTCCTGAAGAGTTATTCCGGCAATGTGGGTCGCTGAGAGTGATTTCTCTGGCGAAAAACAAGTTTTCCGGGAAAATTCCGGAGAGTTTGGGCTCGTGTGCGAGTCTAGCGGCTGTTAACTTTTCGTTGAACCAGTTTTCGGGTTCGATTCCAGCTGGGGTTTGGTCCTTGAGTGGGCTTAGATCCCTGGATTTGTCTGATAACTTGTTGAAGGGTGAAATTCCAAAGGGGATTGAATTGAATAATTTGCGAGGAGTTAATTTGGCCAGGAATCGGTTTACCGGGCAAGTTCCGGATGGAATTGGAAGCTGTTCGCTCTTGAGGTCCATTGATCTGAGTGAGAATTCGTTCTCGGGTAACCTTCCTCAGACAATGCAGAAACTTGGCCTGTGCAGCTATCTGAATCTGCACCAGAACACATTTTCTGGTGAGGTTCCGGAGTGGATTGGAGAGATGAAAAGCCTGGAGACTTTGGACCTTTCTAGCAACCGGTTTACGGGTGAAGTTCCAAGCTCAATAGGCAATCTTGAGGCTTTGAAGGTATTGAAATTTTCTGCAAATGGGTTCACTGGGAGCTTGCCAAAGTCTATGGCTTATTGCACAAACCTTCTGGCTCTAGATTTTAGTAAGAATTCGATGGCGGGTGAACTTCCGGTGTGGATATTCGATGCGGGTGAAGAGGAAGTTTCACTTTCAGATAAAAAACTAAGCGGAAGCAAGAACATCAATCAATCTTTATCAGCTGAAAATGCACTTCAGAATCTCCAAGTCTTGGATTTATCCCTTAATCATTTTTCGGGTGAAATTGCGTCCGACATTGGGGCCTTAAGCAGCTTGCATACGTTGAACCTTTCTGGTAACTCTCTTGCTGGTCCTATTCCTGTGGCTATTGGAGAGTTGAAGGTCCTGAACAATCTCGACTTGAGCGAGAATCGGCTCAATGGAAGCATCCCTCAGGAAATTGGAGGGGCTTTTTCTTTGAAGGAATTGAGATTGGAGAAGAACTTTCTCACGGGAAAAATTCCAACTTCAATTGAGCACTGTTCTTCTCTGACCACATTAACTGTATCACAGAACAGACTGACTGGTCCATTACCTGCAGCAATGTCCAAACTTACCAACTTACAAATTGTGGACTTGTCTTTCAATAACCTCACGGGAGGCCTGCCGAAGCAGTTAGCCAATCTTCCCAACCTACTTTCCTTCAACATTTCCCACAACAACCTCCAGGGTGAACTACCTACGGGTGCCTTTTTCAATACCATTTCCCCTTCCTCCGTCTCTGGAAATCCATCTCTCTGTGGCTCTGCAGTTAACAAGTCTTGCCCGGGAGTCCTTCCCAAGCCCATTGTCCTCAATCCCAATTCCTCTTCCGACTCCACCACAGGAGAAATCTCATCAAATCTTGGTCATAGAAGAATCTTACTCAGTATTTCTTCGCTTATTGCCATTGCAGCAGCCGCTGTCATTGTCATTGGTGTAATTGCCATTACTGTGCTTAATCTCCGTGTCCGATCTCCCACAACTCAATCGGCGCCAGCCCTTGCATTTTCAGGTGGGGATGACTTCAGCCGTTCCCCAACAACAGATGGCAACTCTGGCAAGCTAGTCATGTTTTCAGGTGAGCCTGATTTCAGTACTGGTGCACATGCACTGCTCAACAAGGACTGTGAGCTTGGTCGTGGCGGGTTTGGAGCAGTCTACCGGACACATCTTCAAGATGGACGGCCAGTTGCAATCAAGAAGCTCACTGTTTCTAGTCTTGTCAAGTCTCAAGAAGAATTCGAAAGGGAAGTGAATAAATTGGGGAAAGTGAGGCACGATAATCTTGTGGAGATCGAAGGCTATTACTGGACTCCATCATTACAGCTCATCATACATGATTATGTTGCCGGTGGTAGTTTATATAAGCATCTTCATGATGGAGCAGGTGGAAACTTTCTGTCATGGAATGATCGGTTCAATATAATTCTTGGAACTGCGAAAGGTTTGGCTCACTTGCACCAGATGAACATAATTCACTACAATATAAAGTCCAGTAATGTCCTGATTGGCTGCTCAGGCGAACCGAAAGTGGGAGACTTTGGCCTAGCGAGGTTGTTGCCAATGCTTGACCGATATGTTTTGAGCAGCAAGATCCAGAGTGCACTCGGCTACATGGCACCAGAGTTTGCTTGCAAAACAGTAAAGATAACCGAGAAATGTGATGTGTACGGGTTTGGGGTTTTGGTTCTAGAGATTGTCACGGGGAAAAGACCAGTCGAGTACATGGAAGATGATGTTGTGGTGCTCTGTGACATGGTGAGAGGAGCACTGGAAGAAGGCAGGGTAGAAGAATGCGTTGATGCTCGGCTCCAAGGAATTTTCCCAGCAGAAGAGGCTATACCTATGATGAAACTAGGCTTGATATGCACGTCACAAGTGCCGTCAAACAGACCAGACATGGGTGAGGTTGTCAACATTTTGGAGCTGATCCGATGTCCTTCAGAAGGCCAAGAGGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

104.57

Weight (kDa)

5.94

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 71 1.5e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 100 - 160 8.1e-07 Leucine rich repeat
LRR_14 PF23598 216 - 299 2.6e-06 Leucine-rich repeat region
LRR_14 PF23598 387 - 547 2.7e-11 Leucine-rich repeat region
LRR_8 PF13855 464 - 523 3.9e-07 Leucine rich repeat
LRR_8 PF13855 488 - 547 9.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 693 - 958 2.9e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 694 - 897 3.9e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2333
AasI GACNNNNNNGTC 1 cut(s) 1500
Acc36I ACCTGC 2 cut(s) 1519, 2333
AccB1I GGYRCC 3 cut(s) 1661, 1948, 2575
AccB7I CCANNNNNTGG 1 cut(s) 1186
AccI GTMKAC 2 cut(s) 174, 2115
AccII CGCG 1 cut(s) 71
AccIII TCCGGA 4 cut(s) 303, 493, 706, 850
AciI CCGC 7 cut(s) 26, 524, 1055, 1085, 1125, 1875, 2100
AclWI GGATC 5 cut(s) 134, 213, 591, 2545, 2881
AcoI YGGCCR 3 cut(s) 274, 678, 2141
AcsI RAATTY 8 cut(s) 489, 633, 757, 962, 1045, 1434, 2195, 2775
AcuI CTGAAG 4 cut(s) 441, 1154, 1966, 2883
AcyI GRCGYC 1 cut(s) 1949
AfaI GTAC 3 cut(s) 2053, 2627, 2681
AfiI CCNNNNNNNGG 9 cut(s) 553, 588, 687, 910, 1186, 1233, 2489, 2858, 2903
AflII CTTAAG 1 cut(s) 1240
AflIII ACRYGT 1 cut(s) 102
AgeI ACCGGT 1 cut(s) 903
AjiI CACGTC 2 cut(s) 103, 2829
AjnI CCWGG 4 cut(s) 600, 680, 876, 1641
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
Alw21I GWGCWC 6 cut(s) 240, 1455, 2062, 2563, 2706, 2728
Alw26I GTCTC 5 cut(s) 93, 875, 1699, 2190, 2487
Alw44I GTGCAC 2 cut(s) 2058, 2559
AlwI GGATC 5 cut(s) 134, 213, 591, 2545, 2881
AlwNI CAGNNNCTG 1 cut(s) 1877
Ama87I CYCGRG 3 cut(s) 214, 766, 1741
Aor13HI TCCGGA 4 cut(s) 303, 493, 706, 850
ApaLI GTGCAC 2 cut(s) 2058, 2559
ApeKI GCWGC 8 cut(s) 28, 807, 1245, 1514, 1868, 1871, 2469, 2544
ApoI RAATTY 8 cut(s) 489, 633, 757, 962, 1045, 1434, 2195, 2775
AsiGI ACCGGT 1 cut(s) 903
Asp700I GAANNNNTTC 3 cut(s) 401, 724, 761
AspLEI GCGC 1 cut(s) 1951
AspS9I GGNCC 7 cut(s) 579, 738, 889, 1236, 1286, 1318, 1502
AsuC2I CCSGG 5 cut(s) 227, 484, 697, 1742, 1743
AsuII TTCGAA 2 cut(s) 320, 2199
AsuNHI GCTAGC 1 cut(s) 154
AvaI CYCGRG 3 cut(s) 214, 766, 1741
AvaII GGWCC 6 cut(s) 579, 738, 889, 1286, 1318, 1502
AxyI CCTNAGG 1 cut(s) 1368
BaeGI GKGCMC 2 cut(s) 2062, 2563
BalI TGGCCA 1 cut(s) 680
BanI GGYRCC 3 cut(s) 1661, 1948, 2575
BanII GRGCYC 2 cut(s) 240, 509
BarI GAAGNNNNNNTAC 2 cut(s) 2781, 2813
BauI CACGAG 1 cut(s) 508
Bbv12I GWGCWC 6 cut(s) 240, 1455, 2062, 2563, 2706, 2728
BbvI GCAGC 8 cut(s) 15, 819, 1257, 1526, 1880, 1883, 2456, 2556
BccI CCATC 7 cut(s) 704, 1045, 1714, 1997, 2129, 2281, 2330
BceAI ACGGC 4 cut(s) 29, 1971, 2156, 2824
BcgI CGANNNNNNTGC 4 cut(s) 178, 212, 2497, 2531
BciT130I CCWGG 4 cut(s) 602, 682, 878, 1643
BcnI CCSGG 5 cut(s) 227, 484, 697, 1742, 1743
BcoDI GTCTC 5 cut(s) 93, 875, 1699, 2190, 2487
BfaI CTAG 9 cut(s) 155, 521, 897, 1031, 2021, 2172, 2504, 2648, 2813
BfmI CTRYAG 2 cut(s) 1512, 1722
BfoI RGCGCY 1 cut(s) 1952
BfrI CTTAAG 1 cut(s) 1240
BfuAI ACCTGC 2 cut(s) 1519, 2333
BglII AGATCT 1 cut(s) 74
BlpI GCTNAGC 1 cut(s) 239
BmcAI AGTACT 1 cut(s) 2053
Bme1390I CCNGG 9 cut(s) 227, 484, 602, 682, 697, 878, 1643, 1742, 1743
Bme18I GGWCC 6 cut(s) 579, 738, 889, 1286, 1318, 1502
BmeT110I CYCGRG 3 cut(s) 214, 766, 1741
BmgBI CACGTC 2 cut(s) 103, 2829
BmgT120I GGNCC 7 cut(s) 579, 738, 889, 1236, 1286, 1318, 1502
BmiI GGNNCC 7 cut(s) 60, 849, 1237, 1663, 1950, 2577, 2768
BmrFI CCNGG 9 cut(s) 227, 484, 602, 682, 697, 878, 1643, 1742, 1743
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 4 cut(s) 1072, 1371, 2335, 2749
BmtI GCTAGC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 993
BoxI GACNNNNGTC 1 cut(s) 180
BpmI CTGGAG 2 cut(s) 899, 1625
Bpu10I CCTNAGC 1 cut(s) 2473
Bpu1102I GCTNAGC 1 cut(s) 239
Bpu14I TTCGAA 2 cut(s) 320, 2199
BpuEI CTTGAG 5 cut(s) 604, 754, 962, 1357, 2172
BpuMI CCSGG 5 cut(s) 227, 484, 697, 1742, 1743
BsaHI GRCGYC 1 cut(s) 1949
BsaJI CCNNGG 5 cut(s) 277, 600, 1642, 1741, 2770
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 389, 419
Bsc4I CCNNNNNNNGG 9 cut(s) 553, 588, 687, 910, 1186, 1233, 2489, 2858, 2903
Bse118I RCCGGY 2 cut(s) 903, 2306
Bse1I ACTGG 9 cut(s) 547, 988, 1504, 2059, 2144, 2270, 2451, 2672, 2733
Bse21I CCTNAGG 1 cut(s) 1368
Bse3DI GCAATG 3 cut(s) 443, 1524, 1863
BseAI TCCGGA 4 cut(s) 303, 493, 706, 850
BseBI CCWGG 4 cut(s) 602, 682, 878, 1643
BseDI CCNNGG 5 cut(s) 277, 600, 1642, 1741, 2770
BseGI GGATG 3 cut(s) 715, 1362, 1981
BseLI CCNNNNNNNGG 9 cut(s) 553, 588, 687, 910, 1186, 1233, 2489, 2858, 2903
BseMI GCAATG 3 cut(s) 443, 1524, 1863
BseMII CTCAG 7 cut(s) 253, 440, 740, 794, 1382, 1855, 2487
BseNI ACTGG 9 cut(s) 547, 988, 1504, 2059, 2144, 2270, 2451, 2672, 2733
BseRI GAGGAG 4 cut(s) 23, 26, 681, 2736
BseSI GKGCMC 2 cut(s) 2062, 2563
BseX3I CGGCCG 1 cut(s) 274
BseXI GCAGC 8 cut(s) 15, 819, 1257, 1526, 1880, 1883, 2456, 2556
BseYI CCCAGC 2 cut(s) 569, 2782
BsgI GTGCAG 2 cut(s) 806, 826
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 2 cut(s) 8, 277
BshNI GGYRCC 3 cut(s) 1661, 1948, 2575
BshTI ACCGGT 1 cut(s) 903
BsiEI CGRYCG 2 cut(s) 8, 277
BsiHKAI GWGCWC 6 cut(s) 240, 1455, 2062, 2563, 2706, 2728
BsiHKCI CYCGRG 3 cut(s) 214, 766, 1741
BslI CCNNNNNNNGG 9 cut(s) 553, 588, 687, 910, 1186, 1233, 2489, 2858, 2903
BsmAI GTCTC 5 cut(s) 93, 875, 1699, 2190, 2487
BsmBI CGTCTC 2 cut(s) 93, 1699
BsmI GAATGC 1 cut(s) 2756
BsoBI CYCGRG 3 cut(s) 214, 766, 1741
Bsp119I TTCGAA 2 cut(s) 320, 2199
Bsp1286I GDGCHC 7 cut(s) 240, 509, 1455, 2062, 2563, 2706, 2728
Bsp13I TCCGGA 4 cut(s) 303, 493, 706, 850
Bsp1720I GCTNAGC 1 cut(s) 239
Bsp68I TCGCGA 1 cut(s) 71
BspACI CCGC 7 cut(s) 26, 524, 1055, 1085, 1125, 1875, 2100
BspCNI CTCAG 7 cut(s) 252, 441, 741, 793, 1381, 1854, 2486
BspEI TCCGGA 4 cut(s) 303, 493, 706, 850
BspFNI CGCG 1 cut(s) 71
BspHI TCATGA 1 cut(s) 2332
BspLI GGNNCC 7 cut(s) 60, 849, 1237, 1663, 1950, 2577, 2768
BspMAI CTGCAG 2 cut(s) 1516, 1726
BspMI ACCTGC 2 cut(s) 1519, 2333
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 5 cut(s) 134, 213, 591, 2545, 2881
BspT104I TTCGAA 2 cut(s) 320, 2199
BspT107I GGYRCC 3 cut(s) 1661, 1948, 2575
BspTI CTTAAG 1 cut(s) 1240
BsrDI GCAATG 3 cut(s) 443, 1524, 1863
BsrFI RCCGGY 2 cut(s) 903, 2306
BsrI ACTGG 9 cut(s) 547, 988, 1504, 2059, 2144, 2270, 2451, 2672, 2733
BssAI RCCGGY 2 cut(s) 903, 2306
BssECI CCNNGG 5 cut(s) 277, 600, 1642, 1741, 2770
BssNI GRCGYC 1 cut(s) 1949
BssSI CACGAG 1 cut(s) 508
BssT1I CCWWGG 1 cut(s) 2770
Bst2BI CACGAG 1 cut(s) 508
Bst2UI CCWGG 4 cut(s) 602, 682, 878, 1643
Bst4CI ACNGT 8 cut(s) 9, 182, 1457, 1480, 1909, 2084, 2167, 2599
Bst6I CTCTTC 5 cut(s) 44, 417, 1086, 1789, 2784
BstACI GRCGYC 1 cut(s) 1949
BstAFI CTTAAG 1 cut(s) 1240
BstBI TTCGAA 2 cut(s) 320, 2199
BstC8I GCNNGC 8 cut(s) 156, 297, 992, 1250, 1580, 1953, 2018, 2590
BstDEI CTNAG 9 cut(s) 239, 449, 593, 749, 780, 1121, 1368, 1841, 2473
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 2901
BstF5I GGATG 3 cut(s) 715, 1362, 1981
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 1 cut(s) 1952
BstHHI GCGC 1 cut(s) 1951
BstMAI GTCTC 5 cut(s) 93, 875, 1699, 2190, 2487
BstMCI CGRYCG 2 cut(s) 8, 277
BstNI CCWGG 4 cut(s) 602, 682, 878, 1643
BstNSI RCATGY 1 cut(s) 2066
BstPAI GACNNNNGTC 1 cut(s) 180
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 9 cut(s) 225, 482, 600, 680, 695, 876, 1641, 1740, 1741
BstSFI CTRYAG 2 cut(s) 1512, 1722
BstSLI GKGCMC 2 cut(s) 2062, 2563
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 8 cut(s) 15, 819, 1257, 1526, 1880, 1883, 2456, 2556
BstX2I RGATCY 5 cut(s) 74, 139, 218, 596, 2550
BstYI RGATCY 5 cut(s) 74, 139, 218, 596, 2550
BstZI CGGCCG 1 cut(s) 274
Bsu36I CCTNAGG 1 cut(s) 1368
BtrI CACGTC 2 cut(s) 103, 2829
BtsCI GGATG 3 cut(s) 715, 1362, 1981
BtsI GCAGTG 1 cut(s) 2066
BtsIMutI CAGTG 5 cut(s) 981, 1453, 2066, 2163, 2726
BtuMI TCGCGA 1 cut(s) 71
BveI ACCTGC 2 cut(s) 1519, 2333
Cac8I GCNNGC 8 cut(s) 156, 297, 992, 1250, 1580, 1953, 2018, 2590
CaiI CAGNNNCTG 1 cut(s) 1877
CciI TCATGA 1 cut(s) 2332
CfoI GCGC 1 cut(s) 1951
Cfr10I RCCGGY 2 cut(s) 903, 2306
Cfr13I GGNCC 7 cut(s) 579, 738, 889, 1236, 1286, 1318, 1502
Cfr9I CCCGGG 1 cut(s) 1741
CseI GACGC 2 cut(s) 147, 1212
Csp6I GTAC 3 cut(s) 2052, 2626, 2680
CspAI ACCGGT 1 cut(s) 903
CviQI GTAC 3 cut(s) 2052, 2626, 2680
DdeI CTNAG 9 cut(s) 239, 449, 593, 749, 780, 1121, 1368, 1841, 2473
DinI GGCGCC 1 cut(s) 1950
DrdI GACNNNNNNGTC 1 cut(s) 1500
DseDI GACNNNNNNGTC 1 cut(s) 1500
EaeI YGGCCR 3 cut(s) 274, 678, 2141
EagI CGGCCG 1 cut(s) 274
Eam1104I CTCTTC 5 cut(s) 44, 417, 1086, 1789, 2784
EarI CTCTTC 5 cut(s) 44, 417, 1086, 1789, 2784
Ecl136II GAGCTC 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 2770
Eco147I AGGCCT 2 cut(s) 282, 1578
Eco24I GRGCYC 2 cut(s) 240, 509
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 6 cut(s) 579, 738, 889, 1286, 1318, 1502
Eco52I CGGCCG 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 4 cut(s) 441, 1154, 1966, 2883
Eco81I CCTNAGG 1 cut(s) 1368
Eco88I CYCGRG 3 cut(s) 214, 766, 1741
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 2901
EcoO109I RGGNCCY 2 cut(s) 1236, 1318
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 3 cut(s) 757, 1045, 2195
EcoRII CCWGG 4 cut(s) 600, 680, 876, 1641
EcoRV GATATC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 2770
EcoT38I GRGCYC 2 cut(s) 240, 509
EgeI GGCGCC 1 cut(s) 1950
EheI GGCGCC 1 cut(s) 1950
ErhI CCWWGG 1 cut(s) 2770
Esp3I CGTCTC 2 cut(s) 93, 1699
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FauI CCCGC 3 cut(s) 1048, 1078, 2093
FblI GTMKAC 2 cut(s) 174, 2115
FokI GGATG 3 cut(s) 722, 1349, 1988
FriOI GRGCYC 2 cut(s) 240, 509
FspBI CTAG 9 cut(s) 155, 521, 897, 1031, 2021, 2172, 2504, 2648, 2813
GlaI GCGC 1 cut(s) 1950
GsaI CCCAGC 2 cut(s) 573, 2786
GsuI CTGGAG 2 cut(s) 899, 1625
HaeII RGCGCY 1 cut(s) 1952
HgaI GACGC 2 cut(s) 147, 1212
HhaI GCGC 1 cut(s) 1951
Hin1I GRCGYC 1 cut(s) 1949
Hin6I GCGC 1 cut(s) 1949
HinP1I GCGC 1 cut(s) 1949
HincII GTYRAC 4 cut(s) 175, 532, 1729, 2872
HindII GTYRAC 4 cut(s) 175, 532, 1729, 2872
HindIII AAGCTT 1 cut(s) 340
HpaI GTTAAC 2 cut(s) 532, 1729
Hpy99I CGWCG 6 cut(s) 50, 53, 101, 104, 163, 251
HpyCH4III ACNGT 8 cut(s) 9, 182, 1457, 1480, 1909, 2084, 2167, 2599
HpyCH4IV ACGT 3 cut(s) 102, 1256, 2828
HpyF3I CTNAG 9 cut(s) 239, 449, 593, 749, 780, 1121, 1368, 1841, 2473
HpySE526I ACGT 3 cut(s) 102, 1256, 2828
Hsp92I GRCGYC 1 cut(s) 1949
HspAI GCGC 1 cut(s) 1949
KasI GGCGCC 1 cut(s) 1948
Kpn2I TCCGGA 4 cut(s) 303, 493, 706, 850
KspAI GTTAAC 2 cut(s) 532, 1729
LmnI GCTCC 9 cut(s) 36, 64, 165, 987, 2108, 2339, 2723, 2772, 2881
Lsp1109I GCAGC 8 cut(s) 15, 819, 1257, 1526, 1880, 1883, 2456, 2556
LweI GCATC 4 cut(s) 1072, 1371, 2335, 2749
MaeI CTAG 9 cut(s) 155, 521, 897, 1031, 2021, 2172, 2504, 2648, 2813
MaeII ACGT 3 cut(s) 102, 1256, 2828
MaeIII GTNAC 6 cut(s) 266, 770, 1271, 2656, 2708, 2829
MfeI CAATTG 3 cut(s) 190, 290, 1446
MflI RGATCY 5 cut(s) 74, 139, 218, 596, 2550
MhlI GDGCHC 7 cut(s) 240, 509, 1455, 2062, 2563, 2706, 2728
MlsI TGGCCA 1 cut(s) 680
MluNI TGGCCA 1 cut(s) 680
Mly113I GGCGCC 1 cut(s) 1949
MlyI GAGTC 5 cut(s) 387, 526, 1755, 1784, 2263
MmeI TCCRAC 4 cut(s) 246, 1251, 1463, 1812
Mox20I TGGCCA 1 cut(s) 680
MroI TCCGGA 4 cut(s) 303, 493, 706, 850
MroXI GAANNNNTTC 3 cut(s) 401, 724, 761
MscI TGGCCA 1 cut(s) 680
MseI TTAA 9 cut(s) 123, 344, 531, 672, 1200, 1241, 1475, 1728, 1914
MslI CAYNNNNRTG 3 cut(s) 1907, 2298, 2331
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 4 cut(s) 28, 569, 1157, 1877
MspCI CTTAAG 1 cut(s) 1240
MspR9I CCNGG 9 cut(s) 227, 484, 602, 682, 697, 878, 1643, 1742, 1743
MunI CAATTG 3 cut(s) 190, 290, 1446
Mva1269I GAATGC 1 cut(s) 2756
MvaI CCWGG 4 cut(s) 602, 682, 878, 1643
MvnI CGCG 1 cut(s) 71
NarI GGCGCC 1 cut(s) 1949
NciI CCSGG 5 cut(s) 227, 484, 697, 1742, 1743
NheI GCTAGC 1 cut(s) 154
NlaIV GGNNCC 7 cut(s) 60, 849, 1237, 1663, 1950, 2577, 2768
NmeAIII GCCGAG 3 cut(s) 302, 2544, 2743
NmuCI GTSAC 4 cut(s) 266, 2656, 2708, 2829
NruI TCGCGA 1 cut(s) 71
NspI RCATGY 1 cut(s) 2066
NspV TTCGAA 2 cut(s) 320, 2199
PagI TCATGA 1 cut(s) 2332
PaqCI CACCTGC 1 cut(s) 2333
PceI AGGCCT 2 cut(s) 282, 1578
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 1338
PctI GAATGC 1 cut(s) 2756
PdmI GAANNNNTTC 3 cut(s) 401, 724, 761
PfeI GAWTC 6 cut(s) 562, 685, 817, 1174, 1345, 1833
PflMI CCANNNNNTGG 1 cut(s) 1186
PfoI TCCNGGA 1 cut(s) 482
PinAI ACCGGT 1 cut(s) 903
PleI GAGTC 5 cut(s) 386, 525, 1754, 1784, 2263
PluTI GGCGCC 1 cut(s) 1952
PpsI GAGTC 5 cut(s) 386, 525, 1754, 1784, 2263
PpuMI RGGWCCY 1 cut(s) 1318
PshAI GACNNNNGTC 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 1318
Psp6I CCWGG 4 cut(s) 600, 680, 876, 1641
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 2 cut(s) 569, 2782
PspGI CCWGG 4 cut(s) 600, 680, 876, 1641
PspN4I GGNNCC 7 cut(s) 60, 849, 1237, 1663, 1950, 2577, 2768
PspPI GGNCC 7 cut(s) 579, 738, 889, 1236, 1286, 1318, 1502
PspPPI RGGWCCY 1 cut(s) 1318
PstI CTGCAG 2 cut(s) 1516, 1726
PstNI CAGNNNCTG 1 cut(s) 1877
PsuI RGATCY 5 cut(s) 74, 139, 218, 596, 2550
PvuII CAGCTG 2 cut(s) 569, 1157
RruI TCGCGA 1 cut(s) 71
RsaI GTAC 3 cut(s) 2053, 2627, 2681
RsaNI GTAC 3 cut(s) 2052, 2626, 2680
RseI CAYNNNNRTG 3 cut(s) 1907, 2298, 2331
SacI GAGCTC 1 cut(s) 240
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 9 cut(s) 123, 344, 531, 672, 1200, 1241, 1475, 1728, 1914
Sau96I GGNCC 7 cut(s) 579, 738, 889, 1236, 1286, 1318, 1502
ScaI AGTACT 1 cut(s) 2053
SchI GAGTC 5 cut(s) 387, 526, 1755, 1784, 2263
ScrFI CCNGG 9 cut(s) 227, 484, 602, 682, 697, 878, 1643, 1742, 1743
SduI GDGCHC 7 cut(s) 240, 509, 1455, 2062, 2563, 2706, 2728
SfaNI GCATC 4 cut(s) 1072, 1371, 2335, 2749
SfcI CTRYAG 2 cut(s) 1512, 1722
SfoI GGCGCC 1 cut(s) 1950
SfuI TTCGAA 2 cut(s) 320, 2199
SinI GGWCC 6 cut(s) 579, 738, 889, 1286, 1318, 1502
SmaI CCCGGG 1 cut(s) 1743
SmiMI CAYNNNNRTG 3 cut(s) 1907, 2298, 2331
SmlI CTYRAG 6 cut(s) 583, 733, 941, 1240, 1336, 2187
SmoI CTYRAG 6 cut(s) 583, 733, 941, 1240, 1336, 2187
SseBI AGGCCT 2 cut(s) 282, 1578
SsiI CCGC 7 cut(s) 26, 524, 1055, 1085, 1125, 1875, 2100
SspDI GGCGCC 1 cut(s) 1948
SspMI CTAG 9 cut(s) 155, 521, 897, 1031, 2021, 2172, 2504, 2648, 2813
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 2 cut(s) 282, 1578
StyD4I CCNGG 9 cut(s) 225, 482, 600, 680, 695, 876, 1641, 1740, 1741
StyI CCWWGG 1 cut(s) 2770
TaaI ACNGT 8 cut(s) 9, 182, 1457, 1480, 1909, 2084, 2167, 2599
TaiI ACGT 3 cut(s) 105, 1259, 2831
TaqII GACCGA 1 cut(s) 2544
TatI WGTACW 2 cut(s) 2051, 2679
TauI GCSGC 2 cut(s) 527, 1877
TfiI GAWTC 6 cut(s) 562, 685, 817, 1174, 1345, 1833
Tru1I TTAA 9 cut(s) 123, 344, 531, 672, 1200, 1241, 1475, 1728, 1914
Tru9I TTAA 9 cut(s) 123, 344, 531, 672, 1200, 1241, 1475, 1728, 1914
TscAI CASTG 5 cut(s) 988, 1460, 2073, 2170, 2733
TseFI GTSAC 4 cut(s) 266, 2656, 2708, 2829
TseI GCWGC 8 cut(s) 28, 807, 1245, 1514, 1868, 1871, 2469, 2544
Tsp45I GTSAC 4 cut(s) 266, 2656, 2708, 2829
TspDTI ATGAA 5 cut(s) 35, 884, 2321, 2438, 2822
TspGWI ACGGA 2 cut(s) 1681, 1910
TspMI CCCGGG 1 cut(s) 1741
TspRI CASTG 5 cut(s) 988, 1460, 2073, 2170, 2733
Van91I CCANNNNNTGG 1 cut(s) 1186
Vha464I CTTAAG 1 cut(s) 1240
VneI GTGCAC 2 cut(s) 2058, 2559
VpaK11BI GGWCC 6 cut(s) 579, 738, 889, 1286, 1318, 1502
XagI CCTNNNNNAGG 1 cut(s) 2901
XapI RAATTY 8 cut(s) 489, 633, 757, 962, 1045, 1434, 2195, 2775
XbaI TCTAGA 2 cut(s) 1030, 2647
XceI RCATGY 1 cut(s) 2066
XcmI CCANNNNNNNNNTGG 1 cut(s) 573
XmaI CCCGGG 1 cut(s) 1741
XmiI GTMKAC 2 cut(s) 174, 2115
XmnI GAANNNNTTC 3 cut(s) 401, 724, 761
XspI CTAG 9 cut(s) 155, 521, 897, 1031, 2021, 2172, 2504, 2648, 2813
ZrmI AGTACT 1 cut(s) 2053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.