Rw1G033370
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013923
Physical Location & Seq
Reverse (-)
61467172 .. 61470553
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033370.1

Sequence Viewer

Length: 2832 bp
ATGCGAGAGCAAATAGTGATGAAGCGGCTGCTGGTGTTGCTGGCCGTGTTGGCTCTGGCTCCGATTCTGGGGAGATCCCTGAACCCGTCGTTGAACGACGACGTTTTGGGGCTGATAGTGTTCAAGGCAGACATTCAAGATCCCAAGGCGAAATTGGGTTCGTGGAATGAAGACGACGACAGTCCATGCGGGTGGGTCGGAGTAAAATGTAATCCGAGATCCAACGCCGTCGTCGAGCTCAATCTCGACGGCTTTTCTCTCTCCGGCCACATAGGCCGGGGCCTTCTTCAGTTACAGTCGCTCCGGAAGCTCTCCCTCGCGAAAAACAACCTCACCGGAACCTTAAGCCCCAAGATTGCTCACATCGACAATCTCCGGGTGCTGGATTTGAGTGGGAATGGTTTCTCCGGGGCCGTGCCGGAGGAGTTTTTCCGGCAATGTGGGTCCCTGAGAGTGGTTTCTTTGGCTGGTAACAAGTTCTCCGGGGAGATTCCGGAGAGCCTGGGGTCGTGTGCGGGTCTTGCGACGATTGACTTGAGTTTGAACCGGTTTTCGGGTTCGGTTCCGGCTGGGATTTGGTCCTTGAATGGGATTAGGTCGCTGGACTTGTCTGGGAATTTGTTGGAGGGTGAAATTCCGGAGGACATTGAAGGGTTGAAGAATCTGCGAGCTATTAATTTGGGGAAGAATCGGTTTTCTGGGCTTGTTCCTGATGGAATTGGGAGCTGTTTGCTGTTGAGGTCTGTTGATCTTAGTGAGAATGGGTTCACTGGGAACCTTCCTCAGACAATGCAGAAGCTTGGTCTGTGTGGCTCTTTGAACGTGCAGAGGAACTCGTTTTCGGGTGAGGTTCCTGAGTGGATTGGAGATATGAAGAGCCTGCAGACTTTGGACCTTTCCAGCAACCGGTTTTCGGGTCAAGTTCCGAGTTCATTGAGCAATCTTGAAGCTTTAAAGGTGTTGAATTTTTCTGCTAATGGGTTCACTGGGAGCTTGCCCAAGTCTTTGGTTTATTGCACAAACCTTTTGGCTTTGGATTTTAGTAAGAATTCTTTGGAGGGTGATCTTCCTGACTGGATATTCAAGGCGGGTTTGAAGGAGGTTTTGGTTTCGGAGAAGAAGCCAAACGGAAGTAGCCCTTCGTCTTCCCCAACTGGAAGTGCGCTGCAGAAACTGGAGGTGTTGGATTTATCCGGTAATGGTTTTTCTGGTGAGATTACTTCTGCCATTGGGGCCTTGAGCAGCTTACATGTTTTGAACCTTTCGGACAACGCTCTTGTTGGTCCTGTTCCTGCCATTATTGGAGAGTTGAAGGCCCTGGACAGTCTTGATATGAGTGGGAATCAGCTCAATGGAAGCATTCCTCTGGAAATTGGAGGGGCGATTGTATCACAGAATAGACTCTCTGGCCCCATACCTGCTGCAGTAGGCAAACTTAGCAACTTACAATATGTAGATTTGTCTTTCAATAACCTCTCAGGAGCCCTTCCTAAGCAGCTAGCGAATCTTCCCAACATCTTTTCCTTCAATATTTCCCACAACAACCTGCAGGGCGAACTACCTTCCGGTGGCTTTTTCAACACCATCTCCCCTTATAGTGTCTGGGCCAATCCATCTCTTTGCGGCGCTGCAGTTAATAAGTCATGCCCTGCAGTCCTTCCAAAACCCATTGTCCTCAATCCCAATTCCTCTTCTGACTCCAGCACAGGTGCATTGCCTACAAAATTTGGTCATAAAAGAATCATCCTTAGCATTTCTGCTCTCATTGCAATTGGTGCAGCCGCTTTCATTGTCATTGGTGTTATTGCCATCACCGTGCTTAATCTCCGTGTACGGACTTCCACATCTCGTCCTCCAGCTGCCATCACATTTTCAGGTGGGGATGACTTCAGCAATTCCCCCACTACAGATGCCAACTCTGGTAAACTTGTCATGTTTTCGGGTGACCCTGACTTCAGCACTGGTGCTCATGCTCTACTCAACAAGGACTGTGAGCTTGGACGTGGTGGGTTTGGAGCAGTCTACCGGACAGTACTTCGAGATGGACGGTCTGTTGCAATCAAGAAGCTCACTGTTTCAAGTCTTGTCAAGTCTCAGGAAGAATTTGAGAGAGAAGTTAAGAAATTGGGAAAAGTGAGACACGATAATCTTGTGGAGATTGAAGGCTATTACTGGACTCCATCATTGCAGCTCATCATATACGAATATGTATCTGGTGGTACTTTGTATAAGCATCTTCATGATGGAGCAGGCGGAAACTTCCTTTCATGGAATGATAGGTTTAACATAATTCTTGGAACAGCAAAGAGTTTGGCTCACTTGCACCAGATGAACATAATCCACTACAATATAAAATCTAGCAATGTCCTGATCAGCAGCTCAGGTGAACCCAAAGTAGGAGACTTTGGCCTAGCGAGGTTGTTGCCAATGCTTGACCGATATGTTCTGAGCAGCAAGATCCAGAGCGCCCTTGGCTACATGGCACCAGAGTTTGCTTGCAAAACAGTAAAGATAACCGAGAAATGTGATGTCTACGGGTTTGGTGTTTTGGTTCTAGAGGTGGTTACAGGGAAAAGGCCGGTCGAGTATATGGAAGATGATGTTGTGGTGCTCTGTGACATGGTGAGAGGAGCATTAGAAGAAGGCAGGGTAGAGGAATGTATTGATGCTAGGCTCCAGGGAAGTTTCCCTGCCGAAGAAGCTATTCCGGTCATGAAATTAGGGTTGATATGCACATCACAAGTGCCGTCAAACAGACCCGACATGGGAGAGGTTGTCAACATATTGGAGCTGATCAGATGTCCTTCGGAAGGCCAAGACGAATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

943

Amino Acids

100.5

Weight (kDa)

5.8

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 71 1.3e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 149 - 208 3.3e-06 Leucine rich repeat
LRR_14 PF23598 192 - 348 5.7e-11 Leucine-rich repeat region
LRR_8 PF13855 272 - 327 8.6e-07 Leucine rich repeat
LRR_8 PF13855 391 - 450 4.3e-07 Leucine rich repeat
LRR_8 PF13855 463 - 516 3.8e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 662 - 928 1.3e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 663 - 867 1.4e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1426, 1554
AccB1I GGYRCC 1 cut(s) 2482
AccI GTMKAC 2 cut(s) 2022, 2532
AccII CGCG 1 cut(s) 320
AccIII TCCGGA 3 cut(s) 303, 493, 637
AciI CCGC 7 cut(s) 25, 189, 515, 1088, 1623, 1782, 2253
AclWI GGATC 4 cut(s) 69, 134, 213, 2452
AcoI YGGCCR 2 cut(s) 42, 265
AcsI RAATTY 6 cut(s) 616, 633, 964, 1048, 1724, 2102
AcuI CTGAAG 3 cut(s) 272, 1873, 1939
AfaI GTAC 3 cut(s) 1833, 2034, 2221
AflII CTTAAG 1 cut(s) 343
AflIII ACRYGT 1 cut(s) 1249
AgeI ACCGGT 2 cut(s) 546, 906
AjiI CACGTC 1 cut(s) 2003
AjnI CCWGG 3 cut(s) 501, 1317, 2676
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
Alw21I GWGCWC 3 cut(s) 240, 1969, 2613
Alw26I GTCTC 3 cut(s) 2097, 2131, 2394
AlwI GGATC 4 cut(s) 69, 134, 213, 2452
AlwNI CAGNNNCTG 1 cut(s) 1174
Aor13HI TCCGGA 3 cut(s) 303, 493, 637
ApoI RAATTY 6 cut(s) 616, 633, 964, 1048, 1724, 2102
AseI ATTAAT 1 cut(s) 675
AsiGI ACCGGT 2 cut(s) 546, 906
Asp700I GAANNNNTTC 3 cut(s) 401, 764, 2703
AspLEI GCGC 3 cut(s) 1165, 1628, 2468
AsuC2I CCSGG 4 cut(s) 278, 377, 409, 484
AsuHPI GGTGA 9 cut(s) 325, 641, 857, 1073, 1223, 1804, 1955, 2396, 2635
AsuNHI GCTAGC 1 cut(s) 1498
AvaII GGWCC 4 cut(s) 444, 579, 892, 1283
BanI GGYRCC 1 cut(s) 2482
BanII GRGCYC 2 cut(s) 240, 1486
BbsI GAAGAC 2 cut(s) 177, 1137
Bbv12I GWGCWC 3 cut(s) 240, 1969, 2613
BccI CCATC 8 cut(s) 707, 1592, 1621, 1817, 1871, 2036, 2188, 2237
BceAI ACGGC 5 cut(s) 29, 212, 265, 398, 2731
BcgI CGANNNNNNTGC 2 cut(s) 2404, 2438
BciT130I CCWGG 3 cut(s) 503, 1319, 2678
BclI TGATCA 2 cut(s) 2370, 2793
BcnI CCSGG 4 cut(s) 278, 377, 409, 484
BcoDI GTCTC 3 cut(s) 2097, 2131, 2394
BfaI CTAG 5 cut(s) 1499, 2358, 2411, 2555, 2670
BfmI CTRYAG 7 cut(s) 881, 1166, 1422, 1547, 1629, 1650, 1905
BfoI RGCGCY 2 cut(s) 1629, 2469
BfrI CTTAAG 1 cut(s) 343
BfuAI ACCTGC 2 cut(s) 1426, 1554
BglI GCCNNNNNGGC 3 cut(s) 50, 273, 1232
BmcAI AGTACT 1 cut(s) 2034
Bme1390I CCNGG 7 cut(s) 278, 377, 409, 484, 503, 1319, 2678
Bme18I GGWCC 4 cut(s) 444, 579, 892, 1283
BmgBI CACGTC 1 cut(s) 2003
BmrFI CCNGG 7 cut(s) 278, 377, 409, 484, 503, 1319, 2678
BmrI ACTGGG 2 cut(s) 780, 996
BmsI GCATC 3 cut(s) 1900, 2242, 2656
BmtI GCTAGC 1 cut(s) 1502
BmuI ACTGGG 2 cut(s) 780, 996
BoxI GACNNNNGTC 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 177, 1137
BplI GAGNNNNNCTC 2 cut(s) 2299, 2331
BpmI CTGGAG 4 cut(s) 1196, 1684, 1839, 2660
Bpu10I CCTNAGC 3 cut(s) 1491, 1748, 2380
BpuEI CTTGAG 2 cut(s) 556, 1258
BpuMI CCSGG 4 cut(s) 278, 377, 409, 484
BsaJI CCNNGG 8 cut(s) 144, 277, 408, 483, 502, 1317, 2470, 2677
BsaXI ACNNNNNCTCC 8 cut(s) 285, 315, 464, 494, 1571, 1601, 2760, 2790
Bse118I RCCGGY 3 cut(s) 546, 906, 2578
Bse1I ACTGG 7 cut(s) 775, 991, 1079, 1159, 1179, 1966, 2177
Bse3DI GCAATG 5 cut(s) 443, 1712, 1764, 2183, 2368
BseAI TCCGGA 3 cut(s) 303, 493, 637
BseBI CCWGG 3 cut(s) 503, 1319, 2678
BseDI CCNNGG 8 cut(s) 144, 277, 408, 483, 502, 1317, 2470, 2677
BseGI GGATG 2 cut(s) 1743, 1888
BseMI GCAATG 5 cut(s) 443, 1712, 1764, 2183, 2368
BseMII CTCAG 7 cut(s) 440, 797, 846, 1491, 2108, 2394, 2438
BseNI ACTGG 7 cut(s) 775, 991, 1079, 1159, 1179, 1966, 2177
BseRI GAGGAG 2 cut(s) 437, 2643
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 2 cut(s) 845, 1797
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 2583
BshNI GGYRCC 1 cut(s) 2482
BshTI ACCGGT 2 cut(s) 546, 906
BsiEI CGRYCG 1 cut(s) 2583
BsiHKAI GWGCWC 3 cut(s) 240, 1969, 2613
BslFI GGGAC 1 cut(s) 430
BsmAI GTCTC 3 cut(s) 2097, 2131, 2394
BsmFI GGGAC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 1359
Bsp1286I GDGCHC 4 cut(s) 240, 1486, 1969, 2613
Bsp13I TCCGGA 3 cut(s) 303, 493, 637
Bsp143I GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
Bsp68I TCGCGA 1 cut(s) 320
BspACI CCGC 7 cut(s) 25, 189, 515, 1088, 1623, 1782, 2253
BspCNI CTCAG 7 cut(s) 441, 796, 847, 1490, 2107, 2393, 2439
BspEI TCCGGA 3 cut(s) 303, 493, 637
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 2 cut(s) 2239, 2712
BspMAI CTGCAG 6 cut(s) 885, 1170, 1426, 1551, 1633, 1654
BspMI ACCTGC 2 cut(s) 1426, 1554
BspOI GCTAGC 1 cut(s) 1502
BspPI GGATC 4 cut(s) 69, 134, 213, 2452
BspQI GCTCTTC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 2482
BspTI CTTAAG 1 cut(s) 343
BsrDI GCAATG 5 cut(s) 443, 1712, 1764, 2183, 2368
BsrFI RCCGGY 3 cut(s) 546, 906, 2578
BsrI ACTGG 7 cut(s) 775, 991, 1079, 1159, 1179, 1966, 2177
BssAI RCCGGY 3 cut(s) 546, 906, 2578
BssECI CCNNGG 8 cut(s) 144, 277, 408, 483, 502, 1317, 2470, 2677
BssMI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
BssT1I CCWWGG 2 cut(s) 144, 2470
Bst2UI CCWGG 3 cut(s) 503, 1319, 2678
Bst4CI ACNGT 9 cut(s) 182, 297, 1325, 1816, 1991, 2032, 2049, 2074, 2506
Bst6I CTCTTC 2 cut(s) 869, 1696
BstAFI CTTAAG 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 1775
BstC8I GCNNGC 7 cut(s) 42, 669, 881, 995, 1500, 2251, 2497
BstEII GGTNACC 1 cut(s) 1943
BstENI CCTNNNNNAGG 1 cut(s) 2808
BstF5I GGATG 2 cut(s) 1743, 1888
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 2 cut(s) 1629, 2469
BstHHI GCGC 3 cut(s) 1165, 1628, 2468
BstKTI GATC 8 cut(s) 77, 142, 221, 751, 1066, 2373, 2460, 2796
BstMAI GTCTC 3 cut(s) 2097, 2131, 2394
BstMBI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
BstMCI CGRYCG 1 cut(s) 2583
BstNI CCWGG 3 cut(s) 503, 1319, 2678
BstNSI RCATGY 1 cut(s) 1253
BstPAI GACNNNNGTC 1 cut(s) 180
BstPI GGTNACC 1 cut(s) 1943
BstSCI CCNGG 7 cut(s) 276, 375, 407, 482, 501, 1317, 2676
BstSFI CTRYAG 7 cut(s) 881, 1166, 1422, 1547, 1629, 1650, 1905
BstUI CGCG 1 cut(s) 320
BstV2I GAAGAC 2 cut(s) 177, 1137
BstX2I RGATCY 4 cut(s) 74, 139, 218, 2457
BstXI CCANNNNNNTGG 2 cut(s) 192, 1006
BstYI RGATCY 4 cut(s) 74, 139, 218, 2457
BtrI CACGTC 1 cut(s) 2003
BtsCI GGATG 2 cut(s) 1743, 1888
BtsIMutI CAGTG 4 cut(s) 768, 984, 1959, 2070
BtuMI TCGCGA 1 cut(s) 320
BveI ACCTGC 2 cut(s) 1426, 1554
Cac8I GCNNGC 7 cut(s) 42, 669, 881, 995, 1500, 2251, 2497
CaiI CAGNNNCTG 1 cut(s) 1174
CciI TCATGA 2 cut(s) 2239, 2712
CfoI GCGC 3 cut(s) 1165, 1628, 2468
Cfr10I RCCGGY 3 cut(s) 546, 906, 2578
Csp6I GTAC 3 cut(s) 1832, 2033, 2220
CspAI ACCGGT 2 cut(s) 546, 906
CviQI GTAC 3 cut(s) 1832, 2033, 2220
DpnI GATC 8 cut(s) 76, 141, 220, 750, 1065, 2372, 2459, 2795
DpnII GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 2 cut(s) 42, 265
Eam1104I CTCTTC 2 cut(s) 869, 1696
EarI CTCTTC 2 cut(s) 869, 1696
EciI GGCGGA 1 cut(s) 2268
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 144, 2470
Eco24I GRGCYC 2 cut(s) 240, 1486
Eco47I GGWCC 4 cut(s) 444, 579, 892, 1283
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 3 cut(s) 272, 1873, 1939
Eco91I GGTNACC 1 cut(s) 1943
EcoICRI GAGCTC 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 2808
EcoO109I RGGNCCY 4 cut(s) 280, 444, 1233, 1315
EcoO65I GGTNACC 1 cut(s) 1943
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 3 cut(s) 501, 1317, 2676
EcoT14I CCWWGG 2 cut(s) 144, 2470
EcoT38I GRGCYC 2 cut(s) 240, 1486
ErhI CCWWGG 2 cut(s) 144, 2470
FalI AAGNNNNNCTT 2 cut(s) 1123, 1155
FaqI GGGAC 1 cut(s) 430
FauI CCCGC 3 cut(s) 182, 508, 1081
FbaI TGATCA 2 cut(s) 2370, 2793
FblI GTMKAC 2 cut(s) 2022, 2532
FokI GGATG 2 cut(s) 1730, 1895
FriOI GRGCYC 2 cut(s) 240, 1486
FspBI CTAG 5 cut(s) 1499, 2358, 2411, 2555, 2670
GlaI GCGC 3 cut(s) 1164, 1627, 2467
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 4 cut(s) 1196, 1684, 1839, 2660
HaeII RGCGCY 2 cut(s) 1629, 2469
HhaI GCGC 3 cut(s) 1165, 1628, 2468
Hin6I GCGC 3 cut(s) 1163, 1626, 2466
HinP1I GCGC 3 cut(s) 1163, 1626, 2466
HincII GTYRAC 1 cut(s) 2779
HindII GTYRAC 1 cut(s) 2779
HindIII AAGCTT 2 cut(s) 797, 948
HphI GGTGA 9 cut(s) 325, 641, 857, 1073, 1223, 1804, 1955, 2396, 2635
Hpy166II GTNNAC 8 cut(s) 768, 984, 1832, 1925, 2023, 2387, 2533, 2779
Hpy8I GTNNAC 8 cut(s) 768, 984, 1832, 1925, 2023, 2387, 2533, 2779
Hpy99I CGWCG 8 cut(s) 91, 101, 104, 179, 233, 236, 251, 529
HpyCH4III ACNGT 9 cut(s) 182, 297, 1325, 1816, 1991, 2032, 2049, 2074, 2506
HpyCH4IV ACGT 3 cut(s) 102, 822, 2002
HpySE526I ACGT 3 cut(s) 102, 822, 2002
HspAI GCGC 3 cut(s) 1163, 1626, 2466
KflI GGGWCCC 1 cut(s) 444
Kpn2I TCCGGA 3 cut(s) 303, 493, 637
Ksp22I TGATCA 2 cut(s) 2370, 2793
Kzo9I GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
LguI GCTCTTC 1 cut(s) 869
LweI GCATC 3 cut(s) 1900, 2242, 2656
MaeI CTAG 5 cut(s) 1499, 2358, 2411, 2555, 2670
MaeII ACGT 3 cut(s) 102, 822, 2002
MaeIII GTNAC 5 cut(s) 291, 470, 1943, 2563, 2615
MalI GATC 8 cut(s) 76, 141, 220, 750, 1065, 2372, 2459, 2795
MboI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
MfeI CAATTG 1 cut(s) 1770
MflI RGATCY 4 cut(s) 74, 139, 218, 2457
MhlI GDGCHC 4 cut(s) 240, 1486, 1969, 2613
MlyI GAGTC 3 cut(s) 1395, 1691, 2170
MmeI TCCRAC 4 cut(s) 178, 246, 603, 1164
MroI TCCGGA 3 cut(s) 303, 493, 637
MroXI GAANNNNTTC 3 cut(s) 401, 764, 2703
MseI TTAA 7 cut(s) 344, 675, 953, 1635, 1821, 2118, 2283
MslI CAYNNNNRTG 3 cut(s) 190, 1814, 2238
MspA1I CMGCKG 1 cut(s) 1859
MspCI CTTAAG 1 cut(s) 343
MspR9I CCNGG 7 cut(s) 278, 377, 409, 484, 503, 1319, 2678
MunI CAATTG 1 cut(s) 1770
Mva1269I GAATGC 1 cut(s) 1359
MvaI CCWGG 3 cut(s) 503, 1319, 2678
MvnI CGCG 1 cut(s) 320
NciI CCSGG 4 cut(s) 278, 377, 409, 484
NdeII GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
NheI GCTAGC 1 cut(s) 1498
NmuCI GTSAC 2 cut(s) 1943, 2615
NruI TCGCGA 1 cut(s) 320
NspI RCATGY 1 cut(s) 1253
PagI TCATGA 2 cut(s) 2239, 2712
PciI ACATGT 1 cut(s) 1249
PciSI GCTCTTC 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 1359
PdmI GAANNNNTTC 3 cut(s) 401, 764, 2703
PfeI GAWTC 8 cut(s) 64, 490, 661, 688, 1342, 1504, 1740, 2822
PinAI ACCGGT 2 cut(s) 546, 906
PleI GAGTC 3 cut(s) 1395, 1691, 2170
PpsI GAGTC 3 cut(s) 1395, 1691, 2170
PpuMI RGGWCCY 1 cut(s) 444
PscI ACATGT 1 cut(s) 1249
PshAI GACNNNNGTC 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 444
Psp6I CCWGG 3 cut(s) 501, 1317, 2676
PspEI GGTNACC 1 cut(s) 1943
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 3 cut(s) 501, 1317, 2676
PspPPI RGGWCCY 1 cut(s) 444
PstI CTGCAG 6 cut(s) 885, 1170, 1426, 1551, 1633, 1654
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 4 cut(s) 74, 139, 218, 2457
PvuII CAGCTG 1 cut(s) 1859
RruI TCGCGA 1 cut(s) 320
RsaI GTAC 3 cut(s) 1833, 2034, 2221
RsaNI GTAC 3 cut(s) 1832, 2033, 2220
RseI CAYNNNNRTG 3 cut(s) 190, 1814, 2238
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 869
SaqAI TTAA 7 cut(s) 344, 675, 953, 1635, 1821, 2118, 2283
Sau3AI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2370, 2457, 2793
SbfI CCTGCAGG 1 cut(s) 1551
ScaI AGTACT 1 cut(s) 2034
SchI GAGTC 3 cut(s) 1395, 1691, 2170
ScrFI CCNGG 7 cut(s) 278, 377, 409, 484, 503, 1319, 2678
SdaI CCTGCAGG 1 cut(s) 1551
SduI GDGCHC 4 cut(s) 240, 1486, 1969, 2613
SfaNI GCATC 3 cut(s) 1900, 2242, 2656
SfcI CTRYAG 7 cut(s) 881, 1166, 1422, 1547, 1629, 1650, 1905
SfiI GGCCNNNNNGGCC 1 cut(s) 273
SinI GGWCC 4 cut(s) 444, 579, 892, 1283
SmiMI CAYNNNNRTG 3 cut(s) 190, 1814, 2238
SmlI CTYRAG 3 cut(s) 343, 535, 1237
SmoI CTYRAG 3 cut(s) 343, 535, 1237
Sse8387I CCTGCAGG 1 cut(s) 1551
SsiI CCGC 7 cut(s) 25, 189, 515, 1088, 1623, 1782, 2253
SspI AATATT 1 cut(s) 1531
SspMI CTAG 5 cut(s) 1499, 2358, 2411, 2555, 2670
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 7 cut(s) 276, 375, 407, 482, 501, 1317, 2676
StyI CCWWGG 2 cut(s) 144, 2470
TaaI ACNGT 9 cut(s) 182, 297, 1325, 1816, 1991, 2032, 2049, 2074, 2506
TaiI ACGT 3 cut(s) 105, 825, 2005
TaqI TCGA 5 cut(s) 234, 246, 366, 2038, 2583
TaqII GACCGA 1 cut(s) 2451
TatI WGTACW 1 cut(s) 2032
TauI GCSGC 3 cut(s) 28, 1626, 1784
TfiI GAWTC 8 cut(s) 64, 490, 661, 688, 1342, 1504, 1740, 2822
Tru1I TTAA 7 cut(s) 344, 675, 953, 1635, 1821, 2118, 2283
Tru9I TTAA 7 cut(s) 344, 675, 953, 1635, 1821, 2118, 2283
TscAI CASTG 4 cut(s) 775, 991, 1966, 2077
TseFI GTSAC 2 cut(s) 1943, 2615
Tsp45I GTSAC 2 cut(s) 1943, 2615
TspDTI ATGAA 9 cut(s) 35, 183, 887, 921, 1777, 2228, 2256, 2345, 2729
TspGWI ACGGA 3 cut(s) 1143, 1817, 1849
TspRI CASTG 4 cut(s) 775, 991, 1966, 2077
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 4 cut(s) 444, 579, 892, 1283
VspI ATTAAT 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 2808
XapI RAATTY 6 cut(s) 616, 633, 964, 1048, 1724, 2102
XbaI TCTAGA 1 cut(s) 2554
XceI RCATGY 1 cut(s) 1253
XcmI CCANNNNNNNNNTGG 2 cut(s) 151, 2468
XmiI GTMKAC 2 cut(s) 2022, 2532
XmnI GAANNNNTTC 3 cut(s) 401, 764, 2703
XspI CTAG 5 cut(s) 1499, 2358, 2411, 2555, 2670
ZrmI AGTACT 1 cut(s) 2034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.