Rroxscaffold_4G00285050
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013923
Physical Location & Seq
Forward (+)
6500318 .. 6504227
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285050.1

Sequence Viewer

Length: 2868 bp
ATGCGAGAGCAAATAGTGATGAAGCGGTTGCTGGTGTTGCTGGCCGTGTTTTCTCTGGCTCCGATTCTCGGGAGATCCCTGAACCCGTCGTTAAACGACGACGTTTTGGGGCTGATAGTGTTCAAGGCAGACATCCAAGATCCGAAGGCGAAATTGGGTTCGTGGAATGAAGACGACGACAGTCCATGCGGGTGGGTCGGAGTAAAATGTAATCCGAGATCCAACGCCGTCGTCGAGCTCAATCTCGACGGCTTTTCTCTCTCCGGCCACATAGGCCGGGGCCTTCTTCAGTTACAGTCGCTCCGGAAGCTCTCCCTCTCGAAAAACAACCTCACCGGAACCTTAAGCCCCAAGATTGCTCACATCGACAATCTCCGGGTGCTGGATTTGAGTGGGAATGGTTTCTCCGGGGCCGTGCCGGAGGATTTTTTCCGGCAATGTGGGCCCCTGAGAGTGGTTTCTTTGGCTGATAACAAGTTCTCCGGGGAGATTCCGGAGAGCTTGGGGTCGTGTGCGGGTCTTGCGACGATTGACTTGAGTTTGAACCGGTTTTCGGGTTCGGTTCCGGCTGGGATTTGGTCCTTGAATGGGATTAGGTCGCTGGACTTGTCTGGGAATTTGTTGGAGGGTGAAATTCCAGAGGACATTGAAGGGTTGAAGAATCTGCGAGCTATTAATTTGGGGAAGAATCGGTTTTCTGGGGTTGTTCCTGATGGAATTGGGAGCTGTTTGTTGTTGAGGTCTGTTGATCTTAGTGAGAATGGGTTCACTGGGAACCTTCCTCAGACAATGAAGAAGCTTGGTCTGTGTGGCTCTTTGAACGTGCAGAAGAACTCGTTTTCAGGTGAGGTTCCTGAGTGGATTGGAGAGATGAAGAGCCTGCAGACTTTGGACCTTTCCAGCAACCGGTTTTCGGGTCAAGTTCCGAGTTCATTGGGTAATCTTGAAGCTTTAAAGGTGTTGAATTTTTCTGCTAATGGGTTCACCGGGAGCTTGCCCAAGTCTTTGGTTTATTGCACAAACCTTTTGGCTTTGGATTTTAGTAAGAATTCTTTGGAGGGTGACCTTCCTGAGTGGATATTCAAGGCTGGTCTAAAGGAGGTTTTGGTTTCGGAGAAGAAGCCAAGCGGAAATAGTCCTTCGTCTTCCCCAGCTGGAAGTGCGCTTCAGAAACTGGAGGTGTTGGATTTATCCGGTAATGGTTTTTCTGGTGAGATTACTTCTGCCATTGGGGCCTTGAGCAGCTTACATGTTTTGAACCTTTCGGACAACGCTCTTGTTGGTCCTGTTCCTGCCACTATTGGAGAGTTGAAGGCCCTGGACAGTCTTGATATGAGTGGGAATCAACTAAACGGAAGCATTCCTCTGGAAATTGGAGGGGCATTTTCTTTGAAGGAATTGAGATTGGAAATGAACTTCCTCACTGGAAAAATTCCAACTTCAATTGACAACTGTTCTTCTCTTACCACTTTGATTGTATCACAGAACAGACTCTCTGGCCCCATACCTGCTGCAGAGCCCTTCCTAAGCAGCTGGCAGAATCTTCCCAACATCTTTTCCTTCAATATTTCCCACAACAACCTGCAGGGCGAACTACCTTCGGGTGGCTTTTTCAACACCATCTCCCCTTATAGTGTCTGGGCCAATCCATCTCTTTGCGGCGCTGCAGTTAATAAGTCATGCCCTGCAGTCCTTCCAAAACCCATTGTCCTCAATCCCAATTCCTCTTCCGACTCCAGCACAGGTGCATTGCCTACAAAATTTGGTCATAAAAGAATCATCCTCAGCATTTCTGCTCTCATTGCAATTGGTGCAGCCGCTTTCATTGTCATTGGTGTTATTGCCATCACCGTGCTTAATCTCCGTGTACGGACTTCCACATCTCGTCCTCCAGCTGCCATCACATTTTCGGGTGGGGATGACTTCAGCAATTCCCCCACTACAGATGCCAACTCTGGTAAACTTGTCATGTTTTCGGGTGACCCTGACTTCAGCACTGGTGCTCATGCTCTACTCAACAAGGACTGTGAGCTTGGACGTGGTGGGTTTGGAGCAGTCTACCGGACAGTACTTCGAGATGGTCGGTCTGTTGCAATCAAGAAGCTCACTGTTTCAAGTCTTGTCAAGTCTCAGGAAGAATTTGAGAGGGAAGTTAAGAAATTGGGAAAAGTGAGGCACGATAATCTTGTGGAGATTGAAGGCTATTACTGGACTCCATCATTGCAGCTCATCATATACGAATATGTATCTGGTGGTACTTTGTATAAGCATCTTCACGACGGAGCATGCGGAAACTTCCTTTCATGGAATGATAGGTTCAACATAATTCTTGGAACTGCAAAGAGTTTGGCTCACTTGCACCAGATGAACATAATCCACTACAATATAAAATCTAGCAATGTCCTGATCAGCAGCTCAGGTGAACCCAAAGTGGGAGACTTTGGCCTAGCGAGGTTGTTGCCAATGCTTGACCGATATGTTCTGAGCAGCAAGATCCAGAGCGCCCTTGGCTACATGGCACCAGAGTTTGCTTGCAAAACAGTAAAGATAACCGAGAAATGTGATGTCTACGGGTTTGGTGTTTTGGTTCTAGAGGTGGTTACAGGGAAAAGGCCGGTCGAGTACATGGAAGATGATGTTGTGGTGCTCTGTGACATGGTGAGAGGAGCATTAGAAGAAGGCAGGGTAGAGGAATGTATTGATGCTAGGCTCCAGGGAAGTTTCCCTGCCGAAGAAGCTATTCCGGTCATGAAATTAGGGTTGATATGCACGTCACAAGTGCCGTCAAACAGACCCGACATGGGAGAGGTAGTCAACATATTGGAGCTGATCAGATGTCCTTCAGAAGGCCAAGACGAATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

955

Amino Acids

102.14

Weight (kDa)

5.67

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 71 1.3e-11 Leucine rich repeat N-terminal domain
LRR_4 PF12799 99 - 133 7.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 150 - 208 3.8e-06 Leucine rich repeat
LRR_14 PF23598 192 - 345 2.9e-11 Leucine-rich repeat region
LRR_14 PF23598 382 - 492 3.4e-07 Leucine-rich repeat region
LRR_8 PF13855 391 - 450 5.3e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 674 - 940 1.4e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 675 - 879 1.8e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1516, 1590
AccB1I GGYRCC 1 cut(s) 2518
AccI GTMKAC 2 cut(s) 2058, 2568
AccIII TCCGGA 2 cut(s) 303, 493
AciI CCGC 7 cut(s) 25, 189, 515, 1128, 1659, 1818, 2289
AclWI GGATC 4 cut(s) 69, 134, 213, 2488
AcoI YGGCCR 2 cut(s) 42, 265
AcsI RAATTY 7 cut(s) 616, 633, 964, 1048, 1431, 1760, 2138
AcuI CTGAAG 5 cut(s) 272, 1151, 1909, 1975, 2826
AfaI GTAC 4 cut(s) 1869, 2070, 2257, 2624
AflII CTTAAG 1 cut(s) 343
AflIII ACRYGT 1 cut(s) 1249
AgeI ACCGGT 2 cut(s) 546, 906
AjiI CACGTC 2 cut(s) 2039, 2772
AjnI CCWGG 2 cut(s) 1317, 2712
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
Alw21I GWGCWC 3 cut(s) 240, 2005, 2649
Alw26I GTCTC 2 cut(s) 2133, 2430
AlwI GGATC 4 cut(s) 69, 134, 213, 2488
AlwNI CAGNNNCTG 1 cut(s) 1174
Ama87I CYCGRG 1 cut(s) 68
Aor13HI TCCGGA 2 cut(s) 303, 493
ApaI GGGCCC 1 cut(s) 447
ApeKI GCWGC 9 cut(s) 1242, 1511, 1530, 1664, 1814, 1895, 2224, 2412, 2487
ApoI RAATTY 7 cut(s) 616, 633, 964, 1048, 1431, 1760, 2138
AseI ATTAAT 1 cut(s) 675
AsiGI ACCGGT 2 cut(s) 546, 906
Asp700I GAANNNNTTC 3 cut(s) 401, 764, 2739
AspLEI GCGC 3 cut(s) 1165, 1664, 2504
AsuC2I CCSGG 5 cut(s) 278, 377, 409, 484, 988
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 3 cut(s) 579, 892, 1283
BaeGI GKGCMC 1 cut(s) 447
BanI GGYRCC 1 cut(s) 2518
BanII GRGCYC 3 cut(s) 240, 447, 1521
BbsI GAAGAC 2 cut(s) 177, 1137
Bbv12I GWGCWC 3 cut(s) 240, 2005, 2649
BbvCI CCTCAGC 1 cut(s) 1784
BbvI GCAGC 9 cut(s) 1254, 1498, 1542, 1651, 1826, 1882, 2236, 2424, 2499
BccI CCATC 7 cut(s) 707, 1628, 1657, 1853, 1907, 2072, 2224
BceAI ACGGC 5 cut(s) 29, 212, 265, 398, 2767
BcgI CGANNNNNNTGC 2 cut(s) 2440, 2474
BciT130I CCWGG 2 cut(s) 1319, 2714
BclI TGATCA 2 cut(s) 2406, 2829
BcnI CCSGG 5 cut(s) 278, 377, 409, 484, 988
BcoDI GTCTC 2 cut(s) 2133, 2430
BfaI CTAG 4 cut(s) 2394, 2447, 2591, 2706
BfmI CTRYAG 6 cut(s) 881, 1512, 1583, 1665, 1686, 1941
BfoI RGCGCY 2 cut(s) 1665, 2505
BfrI CTTAAG 1 cut(s) 343
BfuAI ACCTGC 2 cut(s) 1516, 1590
BglI GCCNNNNNGGC 2 cut(s) 273, 1232
BmcAI AGTACT 1 cut(s) 2070
Bme1390I CCNGG 7 cut(s) 278, 377, 409, 484, 988, 1319, 2714
Bme18I GGWCC 3 cut(s) 579, 892, 1283
BmeT110I CYCGRG 1 cut(s) 68
BmgBI CACGTC 2 cut(s) 2039, 2772
BmrFI CCNGG 7 cut(s) 278, 377, 409, 484, 988, 1319, 2714
BmrI ACTGGG 1 cut(s) 780
BmsI GCATC 3 cut(s) 1936, 2278, 2692
BmuI ACTGGG 1 cut(s) 780
BoxI GACNNNNGTC 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 177, 1137
BplI GAGNNNNNCTC 2 cut(s) 2335, 2367
BpmI CTGGAG 4 cut(s) 1196, 1720, 1875, 2696
Bpu10I CCTNAGC 3 cut(s) 1526, 1784, 2416
BpuEI CTTGAG 2 cut(s) 556, 1258
BpuMI CCSGG 5 cut(s) 278, 377, 409, 484, 988
BsaJI CCNNGG 6 cut(s) 277, 408, 483, 1317, 2506, 2713
BsaWI WCCGGW 8 cut(s) 303, 335, 493, 546, 906, 1193, 2061, 2743
BsaXI ACNNNNNCTCC 8 cut(s) 285, 315, 464, 494, 1607, 1637, 2796, 2826
Bse118I RCCGGY 3 cut(s) 546, 906, 2614
Bse1I ACTGG 5 cut(s) 775, 1179, 1429, 2002, 2213
Bse3DI GCAATG 5 cut(s) 443, 1748, 1800, 2219, 2404
BseAI TCCGGA 2 cut(s) 303, 493
BseBI CCWGG 2 cut(s) 1319, 2714
BseDI CCNNGG 6 cut(s) 277, 408, 483, 1317, 2506, 2713
BseGI GGATG 3 cut(s) 132, 1779, 1924
BseMI GCAATG 5 cut(s) 443, 1748, 1800, 2219, 2404
BseMII CTCAG 8 cut(s) 440, 797, 846, 1062, 1798, 2144, 2430, 2474
BseNI ACTGG 5 cut(s) 775, 1179, 1429, 2002, 2213
BseRI GAGGAG 1 cut(s) 2679
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 9 cut(s) 1254, 1498, 1542, 1651, 1826, 1882, 2236, 2424, 2499
BseYI CCCAGC 2 cut(s) 569, 1150
BsgI GTGCAG 2 cut(s) 845, 1833
Bsh1285I CGRYCG 1 cut(s) 2619
BshNI GGYRCC 1 cut(s) 2518
BshTI ACCGGT 2 cut(s) 546, 906
BsiEI CGRYCG 1 cut(s) 2619
BsiHKAI GWGCWC 3 cut(s) 240, 2005, 2649
BsiHKCI CYCGRG 1 cut(s) 68
BsmAI GTCTC 2 cut(s) 2133, 2430
BsmI GAATGC 1 cut(s) 1359
BsoBI CYCGRG 1 cut(s) 68
Bsp120I GGGCCC 1 cut(s) 443
Bsp1286I GDGCHC 5 cut(s) 240, 447, 1521, 2005, 2649
Bsp13I TCCGGA 2 cut(s) 303, 493
Bsp143I GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
BspACI CCGC 7 cut(s) 25, 189, 515, 1128, 1659, 1818, 2289
BspCNI CTCAG 8 cut(s) 441, 796, 847, 1063, 1797, 2143, 2429, 2475
BspEI TCCGGA 2 cut(s) 303, 493
BspHI TCATGA 1 cut(s) 2748
BspMAI CTGCAG 5 cut(s) 885, 1516, 1587, 1669, 1690
BspMI ACCTGC 2 cut(s) 1516, 1590
BspPI GGATC 4 cut(s) 69, 134, 213, 2488
BspQI GCTCTTC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 2518
BspTI CTTAAG 1 cut(s) 343
BsrDI GCAATG 5 cut(s) 443, 1748, 1800, 2219, 2404
BsrFI RCCGGY 3 cut(s) 546, 906, 2614
BsrI ACTGG 5 cut(s) 775, 1179, 1429, 2002, 2213
BssAI RCCGGY 3 cut(s) 546, 906, 2614
BssECI CCNNGG 6 cut(s) 277, 408, 483, 1317, 2506, 2713
BssMI GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
BssT1I CCWWGG 1 cut(s) 2506
Bst2UI CCWGG 2 cut(s) 1319, 2714
Bst4CI ACNGT 9 cut(s) 182, 297, 1325, 1454, 1852, 2027, 2068, 2110, 2542
Bst6I CTCTTC 2 cut(s) 869, 1732
BstAFI CTTAAG 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 1811
BstC8I GCNNGC 7 cut(s) 42, 669, 881, 995, 1535, 2287, 2533
BstEII GGTNACC 2 cut(s) 1061, 1979
BstENI CCTNNNNNAGG 1 cut(s) 2844
BstF5I GGATG 3 cut(s) 132, 1779, 1924
BstH2I RGCGCY 2 cut(s) 1665, 2505
BstHHI GCGC 3 cut(s) 1165, 1664, 2504
BstKTI GATC 7 cut(s) 77, 142, 221, 751, 2409, 2496, 2832
BstMAI GTCTC 2 cut(s) 2133, 2430
BstMBI GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
BstMCI CGRYCG 1 cut(s) 2619
BstNI CCWGG 2 cut(s) 1319, 2714
BstNSI RCATGY 2 cut(s) 1253, 2289
BstPAI GACNNNNGTC 1 cut(s) 180
BstPI GGTNACC 2 cut(s) 1061, 1979
BstSCI CCNGG 7 cut(s) 276, 375, 407, 482, 986, 1317, 2712
BstSFI CTRYAG 6 cut(s) 881, 1512, 1583, 1665, 1686, 1941
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 9 cut(s) 1254, 1498, 1542, 1651, 1826, 1882, 2236, 2424, 2499
BstV2I GAAGAC 2 cut(s) 177, 1137
BstX2I RGATCY 4 cut(s) 74, 139, 218, 2493
BstXI CCANNNNNNTGG 2 cut(s) 192, 1006
BstYI RGATCY 4 cut(s) 74, 139, 218, 2493
BtrI CACGTC 2 cut(s) 2039, 2772
BtsCI GGATG 3 cut(s) 132, 1779, 1924
BtsIMutI CAGTG 4 cut(s) 768, 1422, 1995, 2106
BveI ACCTGC 2 cut(s) 1516, 1590
Cac8I GCNNGC 7 cut(s) 42, 669, 881, 995, 1535, 2287, 2533
CaiI CAGNNNCTG 1 cut(s) 1174
CciI TCATGA 1 cut(s) 2748
CfoI GCGC 3 cut(s) 1165, 1664, 2504
Cfr10I RCCGGY 3 cut(s) 546, 906, 2614
Csp6I GTAC 4 cut(s) 1868, 2069, 2256, 2623
CspAI ACCGGT 2 cut(s) 546, 906
CspCI CAANNNNNGTGG 2 cut(s) 1456, 1491
CviQI GTAC 4 cut(s) 1868, 2069, 2256, 2623
DpnI GATC 7 cut(s) 76, 141, 220, 750, 2408, 2495, 2831
DpnII GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 2 cut(s) 42, 265
Eam1104I CTCTTC 2 cut(s) 869, 1732
EarI CTCTTC 2 cut(s) 869, 1732
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 2506
Eco24I GRGCYC 3 cut(s) 240, 447, 1521
Eco47I GGWCC 3 cut(s) 579, 892, 1283
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 5 cut(s) 272, 1151, 1909, 1975, 2826
Eco88I CYCGRG 1 cut(s) 68
Eco91I GGTNACC 2 cut(s) 1061, 1979
EcoICRI GAGCTC 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 2844
EcoO109I RGGNCCY 4 cut(s) 280, 444, 1233, 1315
EcoO65I GGTNACC 2 cut(s) 1061, 1979
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 2 cut(s) 1317, 2712
EcoT14I CCWWGG 1 cut(s) 2506
EcoT38I GRGCYC 3 cut(s) 240, 447, 1521
ErhI CCWWGG 1 cut(s) 2506
FauI CCCGC 2 cut(s) 182, 508
FbaI TGATCA 2 cut(s) 2406, 2829
FblI GTMKAC 2 cut(s) 2058, 2568
FokI GGATG 3 cut(s) 119, 1766, 1931
FriOI GRGCYC 3 cut(s) 240, 447, 1521
FspBI CTAG 4 cut(s) 2394, 2447, 2591, 2706
GlaI GCGC 3 cut(s) 1164, 1663, 2503
GsaI CCCAGC 2 cut(s) 573, 1154
GsuI CTGGAG 4 cut(s) 1196, 1720, 1875, 2696
HaeII RGCGCY 2 cut(s) 1665, 2505
HhaI GCGC 3 cut(s) 1165, 1664, 2504
Hin6I GCGC 3 cut(s) 1163, 1662, 2502
HinP1I GCGC 3 cut(s) 1163, 1662, 2502
HincII GTYRAC 1 cut(s) 2815
HindII GTYRAC 1 cut(s) 2815
HindIII AAGCTT 2 cut(s) 797, 948
Hpy166II GTNNAC 8 cut(s) 768, 984, 1868, 1961, 2059, 2423, 2569, 2815
Hpy8I GTNNAC 8 cut(s) 768, 984, 1868, 1961, 2059, 2423, 2569, 2815
Hpy99I CGWCG 9 cut(s) 91, 101, 104, 179, 233, 236, 251, 529, 2282
HpyCH4III ACNGT 9 cut(s) 182, 297, 1325, 1454, 1852, 2027, 2068, 2110, 2542
HpyCH4IV ACGT 4 cut(s) 102, 822, 2038, 2771
HpySE526I ACGT 4 cut(s) 102, 822, 2038, 2771
HspAI GCGC 3 cut(s) 1163, 1662, 2502
Kpn2I TCCGGA 2 cut(s) 303, 493
Ksp22I TGATCA 2 cut(s) 2406, 2829
Kzo9I GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
LguI GCTCTTC 1 cut(s) 869
LmnI GCTCC 9 cut(s) 64, 306, 723, 990, 2051, 2282, 2666, 2715, 2824
Lsp1109I GCAGC 9 cut(s) 1254, 1498, 1542, 1651, 1826, 1882, 2236, 2424, 2499
LweI GCATC 3 cut(s) 1936, 2278, 2692
MaeI CTAG 4 cut(s) 2394, 2447, 2591, 2706
MaeII ACGT 4 cut(s) 102, 822, 2038, 2771
MaeIII GTNAC 6 cut(s) 291, 1061, 1979, 2599, 2651, 2772
MalI GATC 7 cut(s) 76, 141, 220, 750, 2408, 2495, 2831
MboI GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
MfeI CAATTG 2 cut(s) 1443, 1806
MflI RGATCY 4 cut(s) 74, 139, 218, 2493
MhlI GDGCHC 5 cut(s) 240, 447, 1521, 2005, 2649
MlyI GAGTC 3 cut(s) 1485, 1727, 2206
MmeI TCCRAC 6 cut(s) 178, 246, 603, 1164, 1460, 1755
MroI TCCGGA 2 cut(s) 303, 493
MroXI GAANNNNTTC 3 cut(s) 401, 764, 2739
MseI TTAA 7 cut(s) 92, 344, 675, 953, 1671, 1857, 2154
MslI CAYNNNNRTG 2 cut(s) 190, 1850
MspA1I CMGCKG 3 cut(s) 1154, 1533, 1895
MspCI CTTAAG 1 cut(s) 343
MspR9I CCNGG 7 cut(s) 278, 377, 409, 484, 988, 1319, 2714
MunI CAATTG 2 cut(s) 1443, 1806
Mva1269I GAATGC 1 cut(s) 1359
MvaI CCWGG 2 cut(s) 1319, 2714
NciI CCSGG 5 cut(s) 278, 377, 409, 484, 988
NdeII GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
NmuCI GTSAC 4 cut(s) 1061, 1979, 2651, 2772
NspI RCATGY 2 cut(s) 1253, 2289
PaeI GCATGC 1 cut(s) 2289
PagI TCATGA 1 cut(s) 2748
PciI ACATGT 1 cut(s) 1249
PciSI GCTCTTC 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 1359
PdmI GAANNNNTTC 3 cut(s) 401, 764, 2739
PfeI GAWTC 8 cut(s) 64, 490, 661, 688, 1342, 1540, 1776, 2858
PinAI ACCGGT 2 cut(s) 546, 906
PleI GAGTC 3 cut(s) 1485, 1727, 2206
PpsI GAGTC 3 cut(s) 1485, 1727, 2206
PscI ACATGT 1 cut(s) 1249
PshAI GACNNNNGTC 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 1317, 2712
PspEI GGTNACC 2 cut(s) 1061, 1979
PspFI CCCAGC 2 cut(s) 569, 1150
PspGI CCWGG 2 cut(s) 1317, 2712
PspOMI GGGCCC 1 cut(s) 443
PstI CTGCAG 5 cut(s) 885, 1516, 1587, 1669, 1690
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 4 cut(s) 74, 139, 218, 2493
PvuII CAGCTG 3 cut(s) 1154, 1533, 1895
RsaI GTAC 4 cut(s) 1869, 2070, 2257, 2624
RsaNI GTAC 4 cut(s) 1868, 2069, 2256, 2623
RseI CAYNNNNRTG 2 cut(s) 190, 1850
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 869
SaqAI TTAA 7 cut(s) 92, 344, 675, 953, 1671, 1857, 2154
Sau3AI GATC 7 cut(s) 74, 139, 218, 748, 2406, 2493, 2829
SbfI CCTGCAGG 1 cut(s) 1587
ScaI AGTACT 1 cut(s) 2070
SchI GAGTC 3 cut(s) 1485, 1727, 2206
ScrFI CCNGG 7 cut(s) 278, 377, 409, 484, 988, 1319, 2714
SdaI CCTGCAGG 1 cut(s) 1587
SduI GDGCHC 5 cut(s) 240, 447, 1521, 2005, 2649
SfaNI GCATC 3 cut(s) 1936, 2278, 2692
SfcI CTRYAG 6 cut(s) 881, 1512, 1583, 1665, 1686, 1941
SfiI GGCCNNNNNGGCC 1 cut(s) 273
SinI GGWCC 3 cut(s) 579, 892, 1283
SmiMI CAYNNNNRTG 2 cut(s) 190, 1850
SmlI CTYRAG 3 cut(s) 343, 535, 1237
SmoI CTYRAG 3 cut(s) 343, 535, 1237
SphI GCATGC 1 cut(s) 2289
Sse8387I CCTGCAGG 1 cut(s) 1587
SsiI CCGC 7 cut(s) 25, 189, 515, 1128, 1659, 1818, 2289
SspI AATATT 1 cut(s) 1567
SspMI CTAG 4 cut(s) 2394, 2447, 2591, 2706
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 7 cut(s) 276, 375, 407, 482, 986, 1317, 2712
StyI CCWWGG 1 cut(s) 2506
TaaI ACNGT 9 cut(s) 182, 297, 1325, 1454, 1852, 2027, 2068, 2110, 2542
TaiI ACGT 4 cut(s) 105, 825, 2041, 2774
TaqI TCGA 6 cut(s) 234, 246, 320, 366, 2074, 2619
TaqII GACCGA 2 cut(s) 2073, 2487
TatI WGTACW 2 cut(s) 2068, 2622
TauI GCSGC 2 cut(s) 1662, 1820
TfiI GAWTC 8 cut(s) 64, 490, 661, 688, 1342, 1540, 1776, 2858
Tru1I TTAA 7 cut(s) 92, 344, 675, 953, 1671, 1857, 2154
Tru9I TTAA 7 cut(s) 92, 344, 675, 953, 1671, 1857, 2154
TscAI CASTG 4 cut(s) 775, 1429, 2002, 2113
TseFI GTSAC 4 cut(s) 1061, 1979, 2651, 2772
TseI GCWGC 9 cut(s) 1242, 1511, 1530, 1664, 1814, 1895, 2224, 2412, 2487
Tsp45I GTSAC 4 cut(s) 1061, 1979, 2651, 2772
TspGWI ACGGA 4 cut(s) 1368, 1853, 1885, 2295
TspRI CASTG 4 cut(s) 775, 1429, 2002, 2113
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 3 cut(s) 579, 892, 1283
VspI ATTAAT 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 2844
XapI RAATTY 7 cut(s) 616, 633, 964, 1048, 1431, 1760, 2138
XbaI TCTAGA 1 cut(s) 2590
XceI RCATGY 2 cut(s) 1253, 2289
XcmI CCANNNNNNNNNTGG 1 cut(s) 2504
XmiI GTMKAC 2 cut(s) 2058, 2568
XmnI GAANNNNTTC 3 cut(s) 401, 764, 2739
XspI CTAG 4 cut(s) 2394, 2447, 2591, 2706
ZrmI AGTACT 1 cut(s) 2070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.