RchiOBHm_Chr1g0372331
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013923
Physical Location & Seq
Reverse (-)
61080284 .. 61084608
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59632

Sequence Viewer

Length: 2922 bp
ATGCGAGAGCAAATAGTGATGAAGCGGCTGCTGGTGTTGCTGGCCGTGTTGGCTCTGGCTCCGATTCTGGGGAGATCCCTGAACCCGTCGTTGAACGACGACGTTTTGGGGCTGATAGTGTTCAAGGCAGACATTCAAGATCCCAAGGCGAAATTGGGTTCGTGGAATGAAGACGACGACAGTCCATGCGGGTGGGTCGGAGTAAAATGTAATCCGAGATCCAACGCCGTCGTCGAGCTCAATCTCGACGGCTTTTCTCTCTCCGGCCACATAGGCCGGGGCCTTCTTCAGTTACAGTCGCTCCGGAAGCTCTCCCTCTCGAAAAACAACCTCACCGGAACCTTAAGCCCCAAGATTGCTCACATCGACAATCTCCGGGTGCTGGATTTGAGTGGGAATGGTTTCTCCGGGGCCGTGCCGGAGGAGTTTTTCCGGCAATGTGGGTCCCTGAGAGTGGTTTCTTTGGCTGGTAACAAGTTCTCCGGGGAGATTCCGGAGAGCTTGGGGTCGTGTGCGGGTCTTGCGACGATTGACTTGAGTTTGAACCGGTTTTCGGGTTCGGTTCCGGCTGGGATTTGGTCCTTGAATGGGATTAGGTCGCTGGACTTGTCTGGGAATTTGTTGGAGGCTGAAATTCCAGAGGACATTGAAGGGTTGAAGAATCTGCGAGCTATTAATTTGGGGAAGAATCGGTTTTCTGGGCTTGTTCCTGATGGAATTGGGAGCTGTTTGCTGTTGAGGTCTGTTGATCTTAGTGAGAATGGGTTCACTGGGAACCTTCCTCAGACAATGCAGAAGCTTGGTCTCTGTGGCTCTTTGAACGTGCAGAGGAACTCGTTTTCGGGTGAGGTTCCTGAGTGGATTGGAGAGATGAAGAGCCTGCAGACTTTGGACCTTTCCAGCAACCGGTTTTCGGGTCAAGTTCCGAGTTCATTGAGCAATCTTGAAGCTTTAAAGGTGTTGAATTTTTCTGCTAATGGGTTCACTGGGAGCTTGCCCAAGTCTTTGGTTTATTGCACAAACCTTTTGGCTTTGGATTTTAGTAAGAATTCTTTGGAGGGTGATCTTCCTGACTGGATATTCAAGGCGGGTTTGAAGGAGGTTTTGGTTTCGGAGAAGAAGCCAAACGGAAGTAGCCCTTTGTCTTCCCCAACTGGAAGTGCGCTGCAGAAACTGGAGGTGTTGGATTTATCCGGTAATGGTTTTTCTGGTGAGATTACTTCTGCCATTGGGGCGTTGAGCAGCTTACTTGTTTTGAACCTTTCGGACAACGCTCTTGTTGGTCCTGTTCCTGCCATTATTGGAGAGTTGAAGGCCCTGGACAGTCTTGATATGAGTGGGAATCAGCTCAATGGAAGCATTCCTCTGGAAATTGGAGGGGCGTTTTCTTTGAAGGAATTAAGATTGGAAAAGAACTTCCTCACTGGAAAAATTCCGACTTCAATTGAGAACTGTTCTTCTCTTACCACTTTGATTGTATCACAGAATAGACTCTCTGGCCCCATACCTGCTGCAGTAGGCAAACTTAGCAACTTACAATATGTAGATTTGTCTTTCAATAACCTCTCAGGAGCCCTTCCTAAGCAGCTGGCGAATCTTCCCAACATCTTTTCCTTCAATATTTCCCACAACAACCTGCAGGGCGAACTACCTTCGGGTGGCTTTTTCAACACCATCTCCCCTTATAGTGTCTGGGCCAATCCATCTCTTTGCGGCGCTGCAGTTAATAAGTCATGCCCTGCAGTCCTTCCAAAACCCATTGTCCTCAATCCCAATTCCTCTTCTGACTCCAGCACAGGTGCATTGCCTACAAAATTTGGTCATAAAAGAATCATCCTCAGCATTTCTGCTCTCATTGCAATTGGTGCAGCTGCTTTCATTGTCATTGGTGTTATTGCCATCACCGTGCTTAATCTCCGTGTACGGACTTCCACATCTCGTCCTCCAGCTGCCATCACATTTTCAGGTGGGGATGACTTCAGCAATTCCCCCACTACAGATGCCAACTCTGGTAAACTTGTCATGTTTTCGGGTGACCCTGACTTCAGCACTGGTGCTCATGCTCTACTCAACAAGGACTGTGAGCTTGGACGTGGTGGGTTTGGAGCAGTCTACCGGACAGTACTTCGAGATGGACGGTCTGTTGCAATCAAGAAGCTCACTGTTTCAAGTCTTGTCAAGTCTCAGGAAGAATTTGAGAGAGAAGTTAAGAAATTGGGAAAAGTGAGACACGATAATCTTGTGGAGATTGAAGGCTATTACTGGACTCCATCATTGCAGCTCATCATATACGAATATGTATCTGGTGGTACTTTGTATAAGCATCTTCACGATGGAGCAGGCGGAAACTTCCTTTCATGGAATGATAGGTTTAACATAATTCTTGGAACAGCAAAGAGTTTGGCTCACTTGCACCAGATGAACATAATCCACTACAATATAAAATCTAGCAATGTCCTGATCAGCAGCTCAGGTGAACCCAAAGTGGGAGACTTTGGCCTAGCGAGGTTGTTGCCAATGCTTGACCGATACGTTCTGAGCAGCAAGATCCAGAGCGCCCTTGGCTACATGGCACCAGAGTTTGCTTGCAAAACAGTAAAGATAACCGAGAAATGTGATGTCTACGGGTTTGGTGTTTTGGTTCTAGAGGTGGTTACAGGGAAAAGGCCGGTCGAGTACATGGAAGATGATGTTGTGGTGCTCTGTGACATGGTGAGAGGAGCATTAGAAGAAGGCAGGGTAGAGGAATGTATTGATGCTAGGCTCCGGGGAAGTTTCCCTGCCGAAGAAGCTATTCCGGTCATGAAATTAGGGTTGATATGCACATCACAAGTGCCGTCAAACAGACCCGACATGGGAGAGGTTGTCAACATATTGGAGCTGATCAGATGTCCTTCAGAGGGCCAAGACGAATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

973

Amino Acids

103.94

Weight (kDa)

5.92

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 71 1.3e-11 Leucine rich repeat N-terminal domain
LRR_4 PF12799 99 - 133 8.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 149 - 208 3.4e-06 Leucine rich repeat
LRR_14 PF23598 196 - 346 1.3e-10 Leucine-rich repeat region
LRR_8 PF13855 272 - 327 3.2e-06 Leucine rich repeat
LRR_14 PF23598 384 - 547 2.5e-10 Leucine-rich repeat region
LRR_8 PF13855 391 - 450 2.3e-07 Leucine rich repeat
LRR_8 PF13855 487 - 546 2.9e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 692 - 958 7.2e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 693 - 897 1.5e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1516, 1644
AccB1I GGYRCC 1 cut(s) 2572
AccI GTMKAC 2 cut(s) 2112, 2622
AccIII TCCGGA 2 cut(s) 303, 493
AciI CCGC 6 cut(s) 25, 189, 515, 1088, 1713, 2343
AclWI GGATC 4 cut(s) 69, 134, 213, 2542
AcoI YGGCCR 2 cut(s) 42, 265
AcsI RAATTY 7 cut(s) 616, 633, 964, 1048, 1431, 1814, 2192
AcuI CTGAAG 4 cut(s) 272, 1963, 2029, 2880
AfaI GTAC 4 cut(s) 1923, 2124, 2311, 2678
AflII CTTAAG 1 cut(s) 343
AgeI ACCGGT 2 cut(s) 546, 906
AjiI CACGTC 1 cut(s) 2093
AjnI CCWGG 1 cut(s) 1317
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
Alw21I GWGCWC 3 cut(s) 240, 2059, 2703
Alw26I GTCTC 4 cut(s) 809, 2187, 2221, 2484
AlwI GGATC 4 cut(s) 69, 134, 213, 2542
AlwNI CAGNNNCTG 1 cut(s) 1174
Aor13HI TCCGGA 2 cut(s) 303, 493
ApoI RAATTY 7 cut(s) 616, 633, 964, 1048, 1431, 1814, 2192
AseI ATTAAT 1 cut(s) 675
AsiGI ACCGGT 2 cut(s) 546, 906
Asp700I GAANNNNTTC 3 cut(s) 401, 764, 2793
AspLEI GCGC 3 cut(s) 1165, 1718, 2558
AsuC2I CCSGG 5 cut(s) 278, 377, 409, 484, 2768
AsuHPI GGTGA 8 cut(s) 325, 857, 1073, 1223, 1894, 2045, 2486, 2725
AvaII GGWCC 4 cut(s) 444, 579, 892, 1283
BanI GGYRCC 1 cut(s) 2572
BanII GRGCYC 2 cut(s) 240, 1576
BbsI GAAGAC 2 cut(s) 177, 1137
Bbv12I GWGCWC 3 cut(s) 240, 2059, 2703
BbvCI CCTCAGC 1 cut(s) 1838
BccI CCATC 8 cut(s) 707, 1682, 1711, 1907, 1961, 2126, 2278, 2327
BceAI ACGGC 5 cut(s) 29, 212, 265, 398, 2821
BcgI CGANNNNNNTGC 2 cut(s) 2494, 2528
BciT130I CCWGG 1 cut(s) 1319
BclI TGATCA 2 cut(s) 2460, 2883
BcnI CCSGG 5 cut(s) 278, 377, 409, 484, 2768
BcoDI GTCTC 4 cut(s) 809, 2187, 2221, 2484
BfaI CTAG 4 cut(s) 2448, 2501, 2645, 2760
BfmI CTRYAG 7 cut(s) 881, 1166, 1512, 1637, 1719, 1740, 1995
BfoI RGCGCY 2 cut(s) 1719, 2559
BfrI CTTAAG 1 cut(s) 343
BfuAI ACCTGC 2 cut(s) 1516, 1644
BglI GCCNNNNNGGC 3 cut(s) 50, 273, 1232
BmcAI AGTACT 1 cut(s) 2124
Bme1390I CCNGG 6 cut(s) 278, 377, 409, 484, 1319, 2768
Bme18I GGWCC 4 cut(s) 444, 579, 892, 1283
BmgBI CACGTC 1 cut(s) 2093
BmrFI CCNGG 6 cut(s) 278, 377, 409, 484, 1319, 2768
BmrI ACTGGG 2 cut(s) 780, 996
BmsI GCATC 3 cut(s) 1990, 2332, 2746
BmuI ACTGGG 2 cut(s) 780, 996
BoxI GACNNNNGTC 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 177, 1137
BplI GAGNNNNNCTC 2 cut(s) 2389, 2421
BpmI CTGGAG 3 cut(s) 1196, 1774, 1929
Bpu10I CCTNAGC 3 cut(s) 1581, 1838, 2470
BpuEI CTTGAG 1 cut(s) 556
BpuMI CCSGG 5 cut(s) 278, 377, 409, 484, 2768
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 7 cut(s) 144, 277, 408, 483, 1317, 2560, 2767
BsaWI WCCGGW 8 cut(s) 303, 335, 493, 546, 906, 1193, 2115, 2797
BsaXI ACNNNNNCTCC 8 cut(s) 285, 315, 464, 494, 1661, 1691, 2850, 2880
Bse118I RCCGGY 3 cut(s) 546, 906, 2668
Bse1I ACTGG 8 cut(s) 775, 991, 1079, 1159, 1179, 1429, 2056, 2267
Bse3DI GCAATG 5 cut(s) 443, 1802, 1854, 2273, 2458
BseAI TCCGGA 2 cut(s) 303, 493
BseBI CCWGG 1 cut(s) 1319
BseDI CCNNGG 7 cut(s) 144, 277, 408, 483, 1317, 2560, 2767
BseGI GGATG 2 cut(s) 1833, 1978
BseMI GCAATG 5 cut(s) 443, 1802, 1854, 2273, 2458
BseMII CTCAG 8 cut(s) 440, 797, 846, 1581, 1852, 2198, 2484, 2528
BseNI ACTGG 8 cut(s) 775, 991, 1079, 1159, 1179, 1429, 2056, 2267
BseRI GAGGAG 2 cut(s) 437, 2733
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 2 cut(s) 845, 1887
Bsh1285I CGRYCG 1 cut(s) 2673
BshNI GGYRCC 1 cut(s) 2572
BshTI ACCGGT 2 cut(s) 546, 906
BsiEI CGRYCG 1 cut(s) 2673
BsiHKAI GWGCWC 3 cut(s) 240, 2059, 2703
BslFI GGGAC 1 cut(s) 430
BsmAI GTCTC 4 cut(s) 809, 2187, 2221, 2484
BsmFI GGGAC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 1359
Bso31I GGTCTC 1 cut(s) 809
Bsp1286I GDGCHC 4 cut(s) 240, 1576, 2059, 2703
Bsp13I TCCGGA 2 cut(s) 303, 493
Bsp143I GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
BspACI CCGC 6 cut(s) 25, 189, 515, 1088, 1713, 2343
BspCNI CTCAG 8 cut(s) 441, 796, 847, 1580, 1851, 2197, 2483, 2529
BspEI TCCGGA 2 cut(s) 303, 493
BspHI TCATGA 1 cut(s) 2802
BspMAI CTGCAG 6 cut(s) 885, 1170, 1516, 1641, 1723, 1744
BspMI ACCTGC 2 cut(s) 1516, 1644
BspPI GGATC 4 cut(s) 69, 134, 213, 2542
BspQI GCTCTTC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 2572
BspTI CTTAAG 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 5 cut(s) 443, 1802, 1854, 2273, 2458
BsrFI RCCGGY 3 cut(s) 546, 906, 2668
BsrI ACTGG 8 cut(s) 775, 991, 1079, 1159, 1179, 1429, 2056, 2267
BssAI RCCGGY 3 cut(s) 546, 906, 2668
BssECI CCNNGG 7 cut(s) 144, 277, 408, 483, 1317, 2560, 2767
BssMI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
BssT1I CCWWGG 2 cut(s) 144, 2560
Bst2UI CCWGG 1 cut(s) 1319
Bst6I CTCTTC 2 cut(s) 869, 1786
BstAFI CTTAAG 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 1865
BstC8I GCNNGC 7 cut(s) 42, 669, 881, 995, 1590, 2341, 2587
BstEII GGTNACC 1 cut(s) 2033
BstF5I GGATG 2 cut(s) 1833, 1978
BstH2I RGCGCY 2 cut(s) 1719, 2559
BstHHI GCGC 3 cut(s) 1165, 1718, 2558
BstKTI GATC 8 cut(s) 77, 142, 221, 751, 1066, 2463, 2550, 2886
BstMAI GTCTC 4 cut(s) 809, 2187, 2221, 2484
BstMBI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
BstMCI CGRYCG 1 cut(s) 2673
BstNI CCWGG 1 cut(s) 1319
BstPAI GACNNNNGTC 1 cut(s) 180
BstPI GGTNACC 1 cut(s) 2033
BstSCI CCNGG 6 cut(s) 276, 375, 407, 482, 1317, 2766
BstSFI CTRYAG 7 cut(s) 881, 1166, 1512, 1637, 1719, 1740, 1995
BstV2I GAAGAC 2 cut(s) 177, 1137
BstX2I RGATCY 4 cut(s) 74, 139, 218, 2547
BstXI CCANNNNNNTGG 2 cut(s) 192, 1006
BstYI RGATCY 4 cut(s) 74, 139, 218, 2547
BtrI CACGTC 1 cut(s) 2093
BtsCI GGATG 2 cut(s) 1833, 1978
BtsIMutI CAGTG 5 cut(s) 768, 984, 1422, 2049, 2160
BveI ACCTGC 2 cut(s) 1516, 1644
Cac8I GCNNGC 7 cut(s) 42, 669, 881, 995, 1590, 2341, 2587
CaiI CAGNNNCTG 1 cut(s) 1174
CciI TCATGA 1 cut(s) 2802
CfoI GCGC 3 cut(s) 1165, 1718, 2558
Cfr10I RCCGGY 3 cut(s) 546, 906, 2668
Csp6I GTAC 4 cut(s) 1922, 2123, 2310, 2677
CspAI ACCGGT 2 cut(s) 546, 906
CspCI CAANNNNNGTGG 2 cut(s) 1456, 1491
CviQI GTAC 4 cut(s) 1922, 2123, 2310, 2677
DpnI GATC 8 cut(s) 76, 141, 220, 750, 1065, 2462, 2549, 2885
DpnII GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 2 cut(s) 42, 265
Eam1104I CTCTTC 2 cut(s) 869, 1786
EarI CTCTTC 2 cut(s) 869, 1786
EciI GGCGGA 1 cut(s) 2358
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 144, 2560
Eco24I GRGCYC 2 cut(s) 240, 1576
Eco31I GGTCTC 1 cut(s) 809
Eco47I GGWCC 4 cut(s) 444, 579, 892, 1283
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 4 cut(s) 272, 1963, 2029, 2880
Eco91I GGTNACC 1 cut(s) 2033
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 3 cut(s) 280, 444, 1315
EcoO65I GGTNACC 1 cut(s) 2033
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 1 cut(s) 1317
EcoT14I CCWWGG 2 cut(s) 144, 2560
EcoT38I GRGCYC 2 cut(s) 240, 1576
ErhI CCWWGG 2 cut(s) 144, 2560
FalI AAGNNNNNCTT 2 cut(s) 1123, 1155
FaqI GGGAC 1 cut(s) 430
FauI CCCGC 3 cut(s) 182, 508, 1081
FbaI TGATCA 2 cut(s) 2460, 2883
FblI GTMKAC 2 cut(s) 2112, 2622
FokI GGATG 2 cut(s) 1820, 1985
FriOI GRGCYC 2 cut(s) 240, 1576
FspBI CTAG 4 cut(s) 2448, 2501, 2645, 2760
GlaI GCGC 3 cut(s) 1164, 1717, 2557
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 3 cut(s) 1196, 1774, 1929
HaeII RGCGCY 2 cut(s) 1719, 2559
HhaI GCGC 3 cut(s) 1165, 1718, 2558
Hin6I GCGC 3 cut(s) 1163, 1716, 2556
HinP1I GCGC 3 cut(s) 1163, 1716, 2556
HincII GTYRAC 1 cut(s) 2869
HindII GTYRAC 1 cut(s) 2869
HindIII AAGCTT 2 cut(s) 797, 948
HphI GGTGA 8 cut(s) 325, 857, 1073, 1223, 1894, 2045, 2486, 2725
Hpy166II GTNNAC 8 cut(s) 768, 984, 1922, 2015, 2113, 2477, 2623, 2869
Hpy8I GTNNAC 8 cut(s) 768, 984, 1922, 2015, 2113, 2477, 2623, 2869
Hpy99I CGWCG 8 cut(s) 91, 101, 104, 179, 233, 236, 251, 529
HpyCH4IV ACGT 4 cut(s) 102, 822, 2092, 2532
HpySE526I ACGT 4 cut(s) 102, 822, 2092, 2532
HspAI GCGC 3 cut(s) 1163, 1716, 2556
KflI GGGWCCC 1 cut(s) 444
Kpn2I TCCGGA 2 cut(s) 303, 493
Ksp22I TGATCA 2 cut(s) 2460, 2883
Kzo9I GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
LguI GCTCTTC 1 cut(s) 869
LweI GCATC 3 cut(s) 1990, 2332, 2746
MaeI CTAG 4 cut(s) 2448, 2501, 2645, 2760
MaeII ACGT 4 cut(s) 102, 822, 2092, 2532
MaeIII GTNAC 5 cut(s) 291, 470, 2033, 2653, 2705
MalI GATC 8 cut(s) 76, 141, 220, 750, 1065, 2462, 2549, 2885
MboI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
MfeI CAATTG 2 cut(s) 1443, 1860
MflI RGATCY 4 cut(s) 74, 139, 218, 2547
MhlI GDGCHC 4 cut(s) 240, 1576, 2059, 2703
MlyI GAGTC 3 cut(s) 1485, 1781, 2260
MmeI TCCRAC 5 cut(s) 178, 246, 603, 1164, 1460
MroI TCCGGA 2 cut(s) 303, 493
MroXI GAANNNNTTC 3 cut(s) 401, 764, 2793
MseI TTAA 8 cut(s) 344, 675, 953, 1400, 1725, 1911, 2208, 2373
MslI CAYNNNNRTG 2 cut(s) 190, 1904
MspA1I CMGCKG 3 cut(s) 1588, 1871, 1949
MspCI CTTAAG 1 cut(s) 343
MspR9I CCNGG 6 cut(s) 278, 377, 409, 484, 1319, 2768
MunI CAATTG 2 cut(s) 1443, 1860
Mva1269I GAATGC 1 cut(s) 1359
MvaI CCWGG 1 cut(s) 1319
NciI CCSGG 5 cut(s) 278, 377, 409, 484, 2768
NdeII GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
NmuCI GTSAC 2 cut(s) 2033, 2705
PagI TCATGA 1 cut(s) 2802
PciSI GCTCTTC 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 1359
PdmI GAANNNNTTC 3 cut(s) 401, 764, 2793
PfeI GAWTC 8 cut(s) 64, 490, 661, 688, 1342, 1594, 1830, 2912
PinAI ACCGGT 2 cut(s) 546, 906
PleI GAGTC 3 cut(s) 1485, 1781, 2260
PpsI GAGTC 3 cut(s) 1485, 1781, 2260
PpuMI RGGWCCY 1 cut(s) 444
PshAI GACNNNNGTC 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 1317
PspEI GGTNACC 1 cut(s) 2033
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 1 cut(s) 1317
PspPPI RGGWCCY 1 cut(s) 444
PstI CTGCAG 6 cut(s) 885, 1170, 1516, 1641, 1723, 1744
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 4 cut(s) 74, 139, 218, 2547
PvuII CAGCTG 3 cut(s) 1588, 1871, 1949
RsaI GTAC 4 cut(s) 1923, 2124, 2311, 2678
RsaNI GTAC 4 cut(s) 1922, 2123, 2310, 2677
RseI CAYNNNNRTG 2 cut(s) 190, 1904
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 869
SaqAI TTAA 8 cut(s) 344, 675, 953, 1400, 1725, 1911, 2208, 2373
Sau3AI GATC 8 cut(s) 74, 139, 218, 748, 1063, 2460, 2547, 2883
SbfI CCTGCAGG 1 cut(s) 1641
ScaI AGTACT 1 cut(s) 2124
SchI GAGTC 3 cut(s) 1485, 1781, 2260
ScrFI CCNGG 6 cut(s) 278, 377, 409, 484, 1319, 2768
SdaI CCTGCAGG 1 cut(s) 1641
SduI GDGCHC 4 cut(s) 240, 1576, 2059, 2703
SfaNI GCATC 3 cut(s) 1990, 2332, 2746
SfcI CTRYAG 7 cut(s) 881, 1166, 1512, 1637, 1719, 1740, 1995
SfiI GGCCNNNNNGGCC 1 cut(s) 273
SinI GGWCC 4 cut(s) 444, 579, 892, 1283
SmiMI CAYNNNNRTG 2 cut(s) 190, 1904
SmlI CTYRAG 2 cut(s) 343, 535
SmoI CTYRAG 2 cut(s) 343, 535
Sse8387I CCTGCAGG 1 cut(s) 1641
SsiI CCGC 6 cut(s) 25, 189, 515, 1088, 1713, 2343
SspI AATATT 1 cut(s) 1621
SspMI CTAG 4 cut(s) 2448, 2501, 2645, 2760
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 6 cut(s) 276, 375, 407, 482, 1317, 2766
StyI CCWWGG 2 cut(s) 144, 2560
TaiI ACGT 4 cut(s) 105, 825, 2095, 2535
TaqI TCGA 6 cut(s) 234, 246, 320, 366, 2128, 2673
TaqII GACCGA 1 cut(s) 2541
TatI WGTACW 2 cut(s) 2122, 2676
TauI GCSGC 2 cut(s) 28, 1716
TfiI GAWTC 8 cut(s) 64, 490, 661, 688, 1342, 1594, 1830, 2912
Tru1I TTAA 8 cut(s) 344, 675, 953, 1400, 1725, 1911, 2208, 2373
Tru9I TTAA 8 cut(s) 344, 675, 953, 1400, 1725, 1911, 2208, 2373
TscAI CASTG 5 cut(s) 775, 991, 1429, 2056, 2167
TseFI GTSAC 2 cut(s) 2033, 2705
Tsp45I GTSAC 2 cut(s) 2033, 2705
TspDTI ATGAA 8 cut(s) 35, 183, 887, 921, 1867, 2346, 2435, 2819
TspGWI ACGGA 3 cut(s) 1143, 1907, 1939
TspRI CASTG 5 cut(s) 775, 991, 1429, 2056, 2167
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 4 cut(s) 444, 579, 892, 1283
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 7 cut(s) 616, 633, 964, 1048, 1431, 1814, 2192
XbaI TCTAGA 1 cut(s) 2644
XcmI CCANNNNNNNNNTGG 2 cut(s) 151, 2558
XmiI GTMKAC 2 cut(s) 2112, 2622
XmnI GAANNNNTTC 3 cut(s) 401, 764, 2793
XspI CTAG 4 cut(s) 2448, 2501, 2645, 2760
ZrmI AGTACT 1 cut(s) 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.