Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011422

Physical Location & Seq

Genomic Coordinates Forward (+)

27305320 .. 27307595

Gene Structure 2276 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1169100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGGAGAAGGATCTTCTGGAGCTGTTCGACGCCGTGAAGAAATCGGCGGACGCCGCCACATCTTGCGACGGAGGAGCCGAGGAAAGCGAATGCCTTGAGGCCTTCGAGCAGCTCAATAATTTTCCCGTCACTCATCAAATCCTCGTCTCAACCCAAGTTGGAAAGCGTCTTCGACATCTCACAAAACATCCTAGAAAAAAAATACAATCGGTTGCTTCAGGCCTTTTGGAGAAATGGAAAGAGATTGTTATGAGCGAGGTAGGGAAAAATGTGAAAAATGGAAACTTGGAAAGAATAGACTCTCTAAAACGTGATTCTCCACGTGCCGACAGCCCCAGGCCTGAGAAGGTTCAGAAGACATCTGCTGTGAAGGTTGAGAAGGTTTCCAAGGCTGAACCTGTTGAGGTTAAGAAGGTTGATCGTGATGTCAAACCAAGCTCTGAGAAAGCTTATAGTTCCGGAACTGTCAAAGCAGAAAGAAAGGTTCAAAATGTTAATCCTGTCAAGACTGAGAAAGCTGCTTCAGTGGAGAATGTGAAGGATGAAAAGGTAGCCAAAGACGTGAAGAAACCAGCACTGAACTCTTCTGGTCCCCCAAAGTTAACCTCTATGATTAAGTGTAATGATACAGCTCGGGACAAAGTGAGGGTACTGCTGCATGAGGCTTTGTCCAAGGTTTCTGAGGAGGGTGATGAAAGGTTTGTGGATGAGGTGAATGCAGCTGATCCTATTCGAGTTGCTGTCACTGTGGAATCTGTGCTGTTTGAGAACTGGGGTGGTTCCACCGGCTCCCAGAAGGCTAAGTATCGATCTTTGATTTTCAACCTGAAGGACCAAAAGAATCCAGATTTCCGCAGAAAAGTTCTGCTTGGACATATCGAGGCAGAGAGGCTCGTGGACCTGTCAACAGCGGAAATGGCAAGTGATCAGAGGCAAGAGGAGAACCAAAAGCTTGAACAAAAAGCATTGTTTGAGTGCGAGCGTGGAGGTGCACCAAAAGCCACAACCGATCAATTCAAATGCGGTCGATGTGGACAGCGCAAGACCACTTACTACCAAATGCAGACCCGGAGTGCGGATGAACCTATGACAACGTATGTAACATGTGTAAACTGCAATAATCGTTGGAAGTTCTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med26 TFIIS_C TFIIS_M

Protein Analysis

380

Amino Acids

42.46

Weight (kDa)

9.0

Isoelectric Point (pI)

34.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med26	PF08711	34 - 83	1.1e-10	TFIIS helical bundle-like domain
TFIIS_M	PF07500	208 - 326	1.1e-32	Transcription factor S-II (TFIIS), central domain
Zn_ribbon_TFIIS	PF01096	339 - 377	9.3e-19	Transcription factor S-II (TFIIS)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38560
fragaria_vesca	FvH4_7g26060 FvH4_7g26060 FvH4_7g26060
malus_domestica	MD01G1169100.v1.1 MD07G1235000.v1.1
prunus_persica	Prupe.2G265300_v2.0.a1
pyrus_communis	pycom01g18180 pycom07g21250
rosa_chinensis	RchiOBHm_Chr1g0372471
rosa_laevigata	RLG00000026896
rosa_multiflora	Rmu_sc0005410.1_g000001 Rmu_sc0011709.1_g000008
rosa_roxburghii	Rroxscaffold_4G00284880
rosa_rugosa	Rorug01G0372200
rosa_samantha	Rh1AG380700 Rh1BG344300 Rh1CG357700 Rh1DG375500
rosa_wichuraiana	Rw1G033490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	458
AciI	CCGC	6 cut(s)	47, 54, 853, 911, 1023, 1076
AclWI	GGATC	2 cut(s)	18, 719
AcuI	CTGAAG	3 cut(s)	201, 507, 848
AcvI	CACGTG	1 cut(s)	323
AcyI	GRCGYC	2 cut(s)	30, 51
AfaI	GTAC	1 cut(s)	651
AfiI	CCNNNNNNNGG	1 cut(s)	1075
AflIII	ACRYGT	1 cut(s)	1103
AgsI	TTSAA	4 cut(s)	488, 823, 956, 1018
AjiI	CACGTC	1 cut(s)	562
AjnI	CCWGG	1 cut(s)	335
AluBI	AGCT	8 cut(s)	22, 112, 438, 449, 518, 632, 722, 952
AluI	AGCT	8 cut(s)	22, 112, 438, 449, 518, 632, 722, 952
Alw21I	GWGCWC	1 cut(s)	994
Alw26I	GTCTC	1 cut(s)	151
Alw44I	GTGCAC	1 cut(s)	990
AlwI	GGATC	2 cut(s)	18, 719
Ama87I	CYCGRG	1 cut(s)	633
Aor13HI	TCCGGA	1 cut(s)	458
AoxI	GGCC	3 cut(s)	99, 220, 338
ApaLI	GTGCAC	1 cut(s)	990
ApeKI	GCWGC	4 cut(s)	109, 518, 655, 719
AspLEI	GCGC	1 cut(s)	1041
AspS9I	GGNCC	3 cut(s)	590, 832, 898
AsuC2I	CCSGG	1 cut(s)	1069
AsuHPI	GGTGA	2 cut(s)	701, 724
AvaI	CYCGRG	1 cut(s)	633
AvaII	GGWCC	3 cut(s)	590, 832, 898
BaeGI	GKGCMC	1 cut(s)	994
BauI	CACGAG	1 cut(s)	893
BbrPI	CACGTG	1 cut(s)	323
BbsI	GAAGAC	2 cut(s)	161, 362
Bbv12I	GWGCWC	1 cut(s)	994
BbvI	GCAGC	4 cut(s)	121, 505, 642, 731
BceAI	ACGGC	1 cut(s)	17
BciT130I	CCWGG	1 cut(s)	337
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	1 cut(s)	1069
BcoDI	GTCTC	1 cut(s)	151
BfaI	CTAG	1 cut(s)	192
BisI	GCNGC	5 cut(s)	54, 110, 519, 656, 720
BlsI	GCNGC	5 cut(s)	55, 111, 520, 657, 721
Bme1390I	CCNGG	2 cut(s)	337, 1069
Bme18I	GGWCC	3 cut(s)	590, 832, 898
BmeT110I	CYCGRG	1 cut(s)	633
BmgBI	CACGTC	1 cut(s)	562
BmgT120I	GGNCC	3 cut(s)	590, 832, 898
BmiI	GGNNCC	4 cut(s)	76, 592, 781, 790
BmrFI	CCNGG	2 cut(s)	337, 1069
BmrI	ACTGGG	1 cut(s)	781
BmuI	ACTGGG	1 cut(s)	781
BpiI	GAAGAC	2 cut(s)	161, 362
BpmI	CTGGAG	1 cut(s)	38
BpuEI	CTTGAG	1 cut(s)	116
BpuMI	CCSGG	1 cut(s)	1069
Bsa29I	ATCGAT	1 cut(s)	808
BsaAI	YACGTR	1 cut(s)	323
BsaHI	GRCGYC	2 cut(s)	30, 51
BsaJI	CCNNGG	4 cut(s)	78, 335, 387, 672
BsaWI	WCCGGW	1 cut(s)	458
Bsc4I	CCNNNNNNNGG	1 cut(s)	1075
Bse118I	RCCGGY	1 cut(s)	785
Bse1I	ACTGG	1 cut(s)	776
BseAI	TCCGGA	1 cut(s)	458
BseBI	CCWGG	1 cut(s)	337
BseCI	ATCGAT	1 cut(s)	808
BseDI	CCNNGG	4 cut(s)	78, 335, 387, 672
BseGI	GGATG	4 cut(s)	187, 547, 712, 1084
BseLI	CCNNNNNNNGG	1 cut(s)	1075
BseMII	CTCAG	4 cut(s)	333, 432, 501, 672
BseNI	ACTGG	1 cut(s)	776
BseRI	GAGGAG	3 cut(s)	87, 698, 953
BseSI	GKGCMC	1 cut(s)	994
BseXI	GCAGC	4 cut(s)	121, 505, 642, 731
Bsh1285I	CGRYCG	1 cut(s)	1027
BshFI	GGCC	3 cut(s)	101, 222, 340
BshVI	ATCGAT	1 cut(s)	808
BsiEI	CGRYCG	1 cut(s)	1027
BsiHKAI	GWGCWC	1 cut(s)	994
BsiHKCI	CYCGRG	1 cut(s)	633
BsiSI	CCGG	3 cut(s)	459, 786, 1069
BslFI	GGGAC	2 cut(s)	576, 650
BslI	CCNNNNNNNGG	1 cut(s)	1075
BsmAI	GTCTC	1 cut(s)	151
BsmBI	CGTCTC	1 cut(s)	151
BsmFI	GGGAC	2 cut(s)	576, 650
BsmI	GAATGC	2 cut(s)	95, 721
BsnI	GGCC	3 cut(s)	101, 222, 340
BsoBI	CYCGRG	1 cut(s)	633
Bsp1286I	GDGCHC	1 cut(s)	994
Bsp13I	TCCGGA	1 cut(s)	458
Bsp143I	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
BspACI	CCGC	6 cut(s)	47, 54, 853, 911, 1023, 1076
BspANI	GGCC	3 cut(s)	101, 222, 340
BspCNI	CTCAG	4 cut(s)	334, 433, 502, 673
BspDI	ATCGAT	1 cut(s)	808
BspEI	TCCGGA	1 cut(s)	458
BspLI	GGNNCC	4 cut(s)	76, 592, 781, 790
BspPI	GGATC	2 cut(s)	18, 719
BsrFI	RCCGGY	1 cut(s)	785
BsrI	ACTGG	1 cut(s)	776
BssAI	RCCGGY	1 cut(s)	785
BssECI	CCNNGG	4 cut(s)	78, 335, 387, 672
BssMI	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
BssNI	GRCGYC	2 cut(s)	30, 51
BssSI	CACGAG	1 cut(s)	893
BssT1I	CCWWGG	2 cut(s)	387, 672
Bst2BI	CACGAG	1 cut(s)	893
Bst2UI	CCWGG	1 cut(s)	337
Bst4CI	ACNGT	2 cut(s)	466, 748
Bst6I	CTCTTC	1 cut(s)	589
BstACI	GRCGYC	2 cut(s)	30, 51
BstBAI	YACGTR	1 cut(s)	323
BstC8I	GCNNGC	1 cut(s)	980
BstDEI	CTNAG	5 cut(s)	342, 441, 510, 681, 801
BstF5I	GGATG	4 cut(s)	187, 547, 712, 1084
BstHHI	GCGC	1 cut(s)	1041
BstKTI	GATC	6 cut(s)	13, 421, 727, 812, 928, 1012
BstMAI	GTCTC	1 cut(s)	151
BstMBI	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
BstMCI	CGRYCG	1 cut(s)	1027
BstMWI	GCNNNNNNNGC	3 cut(s)	53, 917, 998
BstNI	CCWGG	1 cut(s)	337
BstNSI	RCATGY	1 cut(s)	1107
BstSCI	CCNGG	2 cut(s)	335, 1067
BstSLI	GKGCMC	1 cut(s)	994
BstV1I	GCAGC	4 cut(s)	121, 505, 642, 731
BstV2I	GAAGAC	2 cut(s)	161, 362
BstX2I	RGATCY	1 cut(s)	10
BstYI	RGATCY	1 cut(s)	10
Bsu15I	ATCGAT	1 cut(s)	808
BsuRI	GGCC	3 cut(s)	101, 222, 340
BsuTUI	ATCGAT	1 cut(s)	808
BtrI	CACGTC	1 cut(s)	562
BtsCI	GGATG	4 cut(s)	187, 547, 712, 1084
BtsIMutI	CAGTG	3 cut(s)	531, 575, 744
Cac8I	GCNNGC	1 cut(s)	980
CfoI	GCGC	1 cut(s)	1041
Cfr10I	RCCGGY	1 cut(s)	785
Cfr13I	GGNCC	3 cut(s)	590, 832, 898
ClaI	ATCGAT	1 cut(s)	808
CseI	GACGC	3 cut(s)	38, 59, 155
Csp6I	GTAC	1 cut(s)	650
CviAII	CATG	2 cut(s)	659, 1104
CviQI	GTAC	1 cut(s)	650
DdeI	CTNAG	5 cut(s)	342, 441, 510, 681, 801
DpnI	GATC	6 cut(s)	12, 420, 726, 811, 927, 1011
DpnII	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
Eam1104I	CTCTTC	1 cut(s)	589
EarI	CTCTTC	1 cut(s)	589
EciI	GGCGGA	1 cut(s)	62
Eco130I	CCWWGG	2 cut(s)	387, 672
Eco147I	AGGCCT	3 cut(s)	101, 222, 340
Eco47I	GGWCC	3 cut(s)	590, 832, 898
Eco57I	CTGAAG	3 cut(s)	201, 507, 848
Eco72I	CACGTG	1 cut(s)	323
Eco88I	CYCGRG	1 cut(s)	633
EcoRII	CCWGG	1 cut(s)	335
EcoT14I	CCWWGG	2 cut(s)	387, 672
ErhI	CCWWGG	2 cut(s)	387, 672
Esp3I	CGTCTC	1 cut(s)	151
FaeI	CATG	2 cut(s)	662, 1107
FaiI	YATR	8 cut(s)	251, 453, 611, 660, 876, 1088, 1098, 1105
FalI	AAGNNNNNCTT	2 cut(s)	852, 884
FaqI	GGGAC	2 cut(s)	576, 650
FatI	CATG	2 cut(s)	658, 1103
FbaI	TGATCA	1 cut(s)	925
Fnu4HI	GCNGC	5 cut(s)	54, 110, 519, 656, 720
FokI	GGATG	4 cut(s)	174, 554, 719, 1091
Fsp4HI	GCNGC	5 cut(s)	54, 110, 519, 656, 720
FspBI	CTAG	1 cut(s)	192
GlaI	GCGC	1 cut(s)	1040
GluI	GCNGC	5 cut(s)	54, 110, 519, 656, 720
GsuI	CTGGAG	1 cut(s)	38
HaeIII	GGCC	3 cut(s)	101, 222, 340
HapII	CCGG	3 cut(s)	459, 786, 1069
HgaI	GACGC	3 cut(s)	38, 59, 155
HhaI	GCGC	1 cut(s)	1041
Hin1I	GRCGYC	2 cut(s)	30, 51
Hin1II	CATG	2 cut(s)	662, 1107
Hin6I	GCGC	1 cut(s)	1039
HinP1I	GCGC	1 cut(s)	1039
HincII	GTYRAC	2 cut(s)	603, 906
HindII	GTYRAC	2 cut(s)	603, 906
HindIII	AAGCTT	2 cut(s)	447, 950
HinfI	GANTC	4 cut(s)	299, 314, 752, 841
HpaI	GTTAAC	1 cut(s)	603
HpaII	CCGG	3 cut(s)	459, 786, 1069
HphI	GGTGA	2 cut(s)	701, 724
Hpy166II	GTNNAC	6 cut(s)	603, 898, 906, 992, 1034, 1111
Hpy188I	TCNGA	4 cut(s)	354, 442, 682, 930
Hpy188III	TCNNGA	6 cut(s)	17, 422, 459, 505, 635, 845
Hpy8I	GTNNAC	6 cut(s)	603, 898, 906, 992, 1034, 1111
Hpy99I	CGWCG	2 cut(s)	32, 71
HpyAV	CCTTC	8 cut(s)	112, 340, 364, 373, 406, 532, 790, 823
HpyCH4III	ACNGT	2 cut(s)	466, 748
HpyCH4IV	ACGT	4 cut(s)	310, 322, 561, 1094
HpyCH4V	TGCA	5 cut(s)	658, 719, 992, 1063, 1116
HpyF10VI	GCNNNNNNNGC	3 cut(s)	53, 917, 998
HpyF3I	CTNAG	5 cut(s)	342, 441, 510, 681, 801
HpySE526I	ACGT	4 cut(s)	310, 322, 561, 1094
Hsp92I	GRCGYC	2 cut(s)	30, 51
Hsp92II	CATG	2 cut(s)	662, 1107
HspAI	GCGC	1 cut(s)	1039
Kpn2I	TCCGGA	1 cut(s)	458
Ksp22I	TGATCA	1 cut(s)	925
KspAI	GTTAAC	1 cut(s)	603
Kzo9I	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
LmnI	GCTCC	3 cut(s)	19, 74, 794
Lsp1109I	GCAGC	4 cut(s)	121, 505, 642, 731
MaeI	CTAG	1 cut(s)	192
MaeII	ACGT	4 cut(s)	310, 322, 561, 1094
MaeIII	GTNAC	3 cut(s)	127, 742, 1099
MalI	GATC	6 cut(s)	12, 420, 726, 811, 927, 1011
MboI	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
MboII	GAAGA	6 cut(s)	5, 49, 161, 367, 576, 577
MflI	RGATCY	1 cut(s)	10
MhlI	GDGCHC	1 cut(s)	994
MluCI	AATT	2 cut(s)	118, 1013
MlyI	GAGTC	1 cut(s)	293
MmeI	TCCRAC	2 cut(s)	139, 1106
MroI	TCCGGA	1 cut(s)	458
MseI	TTAA	4 cut(s)	408, 495, 602, 615
MspA1I	CMGCKG	2 cut(s)	722, 911
MspI	CCGG	3 cut(s)	459, 786, 1069
MspR9I	CCNGG	2 cut(s)	337, 1069
Mva1269I	GAATGC	2 cut(s)	95, 721
MvaI	CCWGG	1 cut(s)	337
MwoI	GCNNNNNNNGC	3 cut(s)	53, 917, 998
NciI	CCSGG	1 cut(s)	1069
NdeII	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
NlaIII	CATG	2 cut(s)	662, 1107
NlaIV	GGNNCC	4 cut(s)	76, 592, 781, 790
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	2 cut(s)	127, 742
NspI	RCATGY	1 cut(s)	1107
PceI	AGGCCT	3 cut(s)	101, 222, 340
PciI	ACATGT	1 cut(s)	1103
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	2 cut(s)	95, 721
PfeI	GAWTC	3 cut(s)	314, 752, 841
PkrI	GCNGC	5 cut(s)	55, 111, 520, 657, 721
PleI	GAGTC	1 cut(s)	293
PmaCI	CACGTG	1 cut(s)	323
PmlI	CACGTG	1 cut(s)	323
PpsI	GAGTC	1 cut(s)	293
Ppu21I	YACGTR	1 cut(s)	323
PscI	ACATGT	1 cut(s)	1103
Psp6I	CCWGG	1 cut(s)	335
PspCI	CACGTG	1 cut(s)	323
PspGI	CCWGG	1 cut(s)	335
PspN4I	GGNNCC	4 cut(s)	76, 592, 781, 790
PspPI	GGNCC	3 cut(s)	590, 832, 898
PsuI	RGATCY	1 cut(s)	10
PvuII	CAGCTG	1 cut(s)	722
RsaI	GTAC	1 cut(s)	651
RsaNI	GTAC	1 cut(s)	650
SaqAI	TTAA	4 cut(s)	408, 495, 602, 615
SatI	GCNGC	5 cut(s)	54, 110, 519, 656, 720
Sau3AI	GATC	6 cut(s)	10, 418, 724, 809, 925, 1009
Sau96I	GGNCC	3 cut(s)	590, 832, 898
SchI	GAGTC	1 cut(s)	293
ScrFI	CCNGG	2 cut(s)	337, 1069
SduI	GDGCHC	1 cut(s)	994
SinI	GGWCC	3 cut(s)	590, 832, 898
SmlI	CTYRAG	1 cut(s)	95
SmoI	CTYRAG	1 cut(s)	95
Sse9I	AATT	2 cut(s)	118, 1013
SseBI	AGGCCT	3 cut(s)	101, 222, 340
SsiI	CCGC	6 cut(s)	47, 54, 853, 911, 1023, 1076
SspMI	CTAG	1 cut(s)	192
StuI	AGGCCT	3 cut(s)	101, 222, 340
StyD4I	CCNGG	2 cut(s)	335, 1067
StyI	CCWWGG	2 cut(s)	387, 672
TaaI	ACNGT	2 cut(s)	466, 748
TaiI	ACGT	4 cut(s)	313, 325, 564, 1097
TaqI	TCGA	7 cut(s)	27, 105, 172, 733, 808, 879, 1027
TasI	AATT	2 cut(s)	118, 1013
TauI	GCSGC	1 cut(s)	56
TfiI	GAWTC	3 cut(s)	314, 752, 841
Tru1I	TTAA	4 cut(s)	408, 495, 602, 615
Tru9I	TTAA	4 cut(s)	408, 495, 602, 615
TscAI	CASTG	3 cut(s)	531, 582, 751
TseFI	GTSAC	2 cut(s)	127, 742
TseI	GCWGC	4 cut(s)	109, 518, 655, 719
Tsp45I	GTSAC	2 cut(s)	127, 742
TspDTI	ATGAA	3 cut(s)	558, 708, 1095
TspGWI	ACGGA	1 cut(s)	84
TspRI	CASTG	3 cut(s)	531, 582, 751
VneI	GTGCAC	1 cut(s)	990
VpaK11BI	GGWCC	3 cut(s)	590, 832, 898
XceI	RCATGY	1 cut(s)	1107
XspI	CTAG	1 cut(s)	192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.