Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0011422

Physical Location & Seq

Genomic Coordinates Forward (+)

30834591 .. 30837411

Gene Structure 2821 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1235000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGGAGAAGGAGCTTCTGGAGTTGTTCGACGCCGTGAAGAAGACGGCGGACGCCGCCACATCTTGCGACGGAGGAATCGAGGAAACCCAATGCCTTGAGGCCTTCGAGCAGCTCAGGAATTTTCCCGTCACTCATCAAATCCTCGTCTCAACCCAAGTTGGGAAGCGTCTTCGACATCTCACAAAGCATCCTAGAAAGAAAATCCAGTCTGTTGCTTCTGGCCTGTTGGATAAATGGAAAGAGATTGTTATGATCGAGGTAGAAAAAACTGCGAAAAATGTGAACTTGGAGAGAATAGACTCTCTAAAACGTGATTCTCCACGTGCCGACAGCCCCAGGCCTGAGAAGGTTCAGAAGACATCTGCTGTGAAGGTTGAGAAGGTTTCCAAGGCTGAACCTGTTGAGGTTAAGAAGGTTGATCGTGATGTCAAACCAAGATCTGAGAATGCTTATGGTTCCGGAGCTGTCAAAACAGAAAGGAAGGTTCCAAATCCTAATCCTGTCAGGACTGAGAAAGCTGCTTCAGCGGAGACTGTTAAGGTTGAAATGATAGCCAAAGAAGTGAAGAAACCAGCACTCAACTCCTCTGATCCCCCAAAGTTAACCTCTATGATTAAGTGTAATGATACAGCTCGGGACAAAGTAAGGGTACTGCTGCATGAGGCCTTGTCAAAGGTTGCTGCGGAGGGTGATGAAAGGTTTGCCGATGAGGTGAATGCAGCTGATCCTATTCGAGTTGCTGTCACTGTGGAATCTGTGCTCTTTGAGAATTGGGGTGGTTCCACCGGCTCTCAGAAGGCTAAGTATCGATCTTTGATTTTCAACCTGAAGGACCAAAAGAACCTAGATTTCCGGAGAAAAGTTCTGCTTGGACATATCAAGGCAGAGAGGCTTGTGAACCTGTCAACAGCGGAAATGGCAAGTGATCAGAGGCAAGAGGAGACCAAGAAGCTTGAAGAAAAAGCATTGTTTGAGTGCGAGCGTGGAGGGCCACCAAAAGCGACAACCGATCAATTCAAATGTGGTCGGTGTGGGCAGCGCAAGACCACCTACTACCAAATGCAGACCCGGAGTGCGGATGAACCTATGACAACATATGTAACATGTGTAAACTGCAATAACCGTTGGAAGTTCTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med26 TFIIS_C TFIIS_M

Protein Analysis

380

Amino Acids

42.65

Weight (kDa)

9.24

Isoelectric Point (pI)

33.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med26	PF08711	34 - 83	1.2e-10	TFIIS helical bundle-like domain
TFIIS_M	PF07500	208 - 326	3e-31	Transcription factor S-II (TFIIS), central domain
Zn_ribbon_TFIIS	PF01096	339 - 377	9.3e-19	Transcription factor S-II (TFIIS)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38560
fragaria_vesca	FvH4_7g26060 FvH4_7g26060 FvH4_7g26060
malus_domestica	MD01G1169100.v1.1 MD07G1235000.v1.1
prunus_persica	Prupe.2G265300_v2.0.a1
pyrus_communis	pycom01g18180 pycom07g21250
rosa_chinensis	RchiOBHm_Chr1g0372471
rosa_laevigata	RLG00000026896
rosa_multiflora	Rmu_sc0005410.1_g000001 Rmu_sc0011709.1_g000008
rosa_roxburghii	Rroxscaffold_4G00284880
rosa_rugosa	Rorug01G0372200
rosa_samantha	Rh1AG380700 Rh1BG344300 Rh1CG357700 Rh1DG375500
rosa_wichuraiana	Rw1G033490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	2 cut(s)	458, 852
AciI	CCGC	6 cut(s)	47, 54, 527, 683, 911, 1076
AclWI	GGATC	2 cut(s)	584, 719
AcsI	RAATTY	1 cut(s)	118
AcuI	CTGAAG	2 cut(s)	507, 848
AcvI	CACGTG	1 cut(s)	323
AcyI	GRCGYC	2 cut(s)	30, 51
AfaI	GTAC	1 cut(s)	651
AfiI	CCNNNNNNNGG	2 cut(s)	159, 1075
AflIII	ACRYGT	1 cut(s)	1103
AgsI	TTSAA	4 cut(s)	545, 823, 956, 1018
AjnI	CCWGG	1 cut(s)	335
AluBI	AGCT	7 cut(s)	13, 112, 464, 518, 632, 722, 952
AluI	AGCT	7 cut(s)	13, 112, 464, 518, 632, 722, 952
Alw21I	GWGCWC	1 cut(s)	762
Alw26I	GTCTC	3 cut(s)	151, 524, 935
AlwI	GGATC	2 cut(s)	584, 719
Ama87I	CYCGRG	1 cut(s)	633
Aor13HI	TCCGGA	2 cut(s)	458, 852
AoxI	GGCC	5 cut(s)	99, 220, 338, 663, 989
ApeKI	GCWGC	6 cut(s)	109, 518, 655, 680, 719, 1036
ApoI	RAATTY	1 cut(s)	118
AspLEI	GCGC	1 cut(s)	1041
AspS9I	GGNCC	2 cut(s)	832, 989
AsuC2I	CCSGG	1 cut(s)	1069
AsuHPI	GGTGA	2 cut(s)	701, 724
AvaI	CYCGRG	1 cut(s)	633
AvaII	GGWCC	1 cut(s)	832
BbrPI	CACGTG	1 cut(s)	323
BbsI	GAAGAC	3 cut(s)	47, 161, 362
Bbv12I	GWGCWC	1 cut(s)	762
BbvI	GCAGC	6 cut(s)	121, 505, 642, 667, 731, 1048
BceAI	ACGGC	2 cut(s)	17, 60
BciT130I	CCWGG	1 cut(s)	337
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	1 cut(s)	1069
BcoDI	GTCTC	3 cut(s)	151, 524, 935
BfaI	CTAG	2 cut(s)	192, 845
BglII	AGATCT	1 cut(s)	437
BisI	GCNGC	7 cut(s)	54, 110, 519, 656, 681, 720, 1037
BlsI	GCNGC	7 cut(s)	55, 111, 520, 657, 682, 721, 1038
Bme1390I	CCNGG	2 cut(s)	337, 1069
Bme18I	GGWCC	1 cut(s)	832
BmeT110I	CYCGRG	1 cut(s)	633
BmgT120I	GGNCC	2 cut(s)	832, 989
BmiI	GGNNCC	3 cut(s)	457, 486, 781
BmrFI	CCNGG	2 cut(s)	337, 1069
BmsI	GCATC	1 cut(s)	196
BpiI	GAAGAC	3 cut(s)	47, 161, 362
BpmI	CTGGAG	1 cut(s)	38
Bpu10I	CCTNAGC	1 cut(s)	113
BpuEI	CTTGAG	1 cut(s)	116
BpuMI	CCSGG	1 cut(s)	1069
Bsa29I	ATCGAT	1 cut(s)	808
BsaAI	YACGTR	1 cut(s)	323
BsaHI	GRCGYC	2 cut(s)	30, 51
BsaI	GGTCTC	1 cut(s)	935
BsaJI	CCNNGG	2 cut(s)	335, 387
BsaWI	WCCGGW	2 cut(s)	458, 852
Bsc4I	CCNNNNNNNGG	2 cut(s)	159, 1075
Bse118I	RCCGGY	1 cut(s)	785
Bse1I	ACTGG	1 cut(s)	205
BseAI	TCCGGA	2 cut(s)	458, 852
BseBI	CCWGG	1 cut(s)	337
BseCI	ATCGAT	1 cut(s)	808
BseDI	CCNNGG	2 cut(s)	335, 387
BseGI	GGATG	2 cut(s)	187, 1084
BseLI	CCNNNNNNNGG	2 cut(s)	159, 1075
BseMII	CTCAG	5 cut(s)	127, 333, 432, 501, 806
BseNI	ACTGG	1 cut(s)	205
BseRI	GAGGAG	2 cut(s)	574, 953
BseXI	GCAGC	6 cut(s)	121, 505, 642, 667, 731, 1048
BshFI	GGCC	5 cut(s)	101, 222, 340, 665, 991
BshVI	ATCGAT	1 cut(s)	808
BsiHKAI	GWGCWC	1 cut(s)	762
BsiHKCI	CYCGRG	1 cut(s)	633
BsiSI	CCGG	4 cut(s)	459, 786, 853, 1069
BslFI	GGGAC	1 cut(s)	650
BslI	CCNNNNNNNGG	2 cut(s)	159, 1075
BsmAI	GTCTC	3 cut(s)	151, 524, 935
BsmBI	CGTCTC	1 cut(s)	151
BsmFI	GGGAC	1 cut(s)	650
BsmI	GAATGC	2 cut(s)	451, 721
BsnI	GGCC	5 cut(s)	101, 222, 340, 665, 991
Bso31I	GGTCTC	1 cut(s)	935
BsoBI	CYCGRG	1 cut(s)	633
Bsp1286I	GDGCHC	1 cut(s)	762
Bsp13I	TCCGGA	2 cut(s)	458, 852
Bsp143I	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
BspACI	CCGC	6 cut(s)	47, 54, 527, 683, 911, 1076
BspANI	GGCC	5 cut(s)	101, 222, 340, 665, 991
BspCNI	CTCAG	5 cut(s)	126, 334, 433, 502, 805
BspDI	ATCGAT	1 cut(s)	808
BspEI	TCCGGA	2 cut(s)	458, 852
BspLI	GGNNCC	3 cut(s)	457, 486, 781
BspPI	GGATC	2 cut(s)	584, 719
BspTNI	GGTCTC	1 cut(s)	935
BsrFI	RCCGGY	1 cut(s)	785
BsrI	ACTGG	1 cut(s)	205
BssAI	RCCGGY	1 cut(s)	785
BssECI	CCNNGG	2 cut(s)	335, 387
BssMI	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
BssNI	GRCGYC	2 cut(s)	30, 51
BssT1I	CCWWGG	1 cut(s)	387
Bst2UI	CCWGG	1 cut(s)	337
Bst4CI	ACNGT	3 cut(s)	535, 748, 1124
BstACI	GRCGYC	2 cut(s)	30, 51
BstBAI	YACGTR	1 cut(s)	323
BstC8I	GCNNGC	1 cut(s)	980
BstDEI	CTNAG	6 cut(s)	113, 342, 441, 510, 792, 801
BstF5I	GGATG	2 cut(s)	187, 1084
BstHHI	GCGC	1 cut(s)	1041
BstKTI	GATC	8 cut(s)	255, 421, 440, 592, 727, 812, 928, 1012
BstMAI	GTCTC	3 cut(s)	151, 524, 935
BstMBI	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
BstMWI	GCNNNNNNNGC	4 cut(s)	53, 524, 917, 988
BstNI	CCWGG	1 cut(s)	337
BstNSI	RCATGY	1 cut(s)	1107
BstSCI	CCNGG	2 cut(s)	335, 1067
BstV1I	GCAGC	6 cut(s)	121, 505, 642, 667, 731, 1048
BstV2I	GAAGAC	3 cut(s)	47, 161, 362
BstX2I	RGATCY	1 cut(s)	437
BstYI	RGATCY	1 cut(s)	437
Bsu15I	ATCGAT	1 cut(s)	808
BsuRI	GGCC	5 cut(s)	101, 222, 340, 665, 991
BsuTUI	ATCGAT	1 cut(s)	808
BtsCI	GGATG	2 cut(s)	187, 1084
BtsIMutI	CAGTG	1 cut(s)	744
Cac8I	GCNNGC	1 cut(s)	980
CfoI	GCGC	1 cut(s)	1041
Cfr10I	RCCGGY	1 cut(s)	785
Cfr13I	GGNCC	2 cut(s)	832, 989
ClaI	ATCGAT	1 cut(s)	808
CseI	GACGC	3 cut(s)	38, 59, 155
Csp6I	GTAC	1 cut(s)	650
CviAII	CATG	2 cut(s)	659, 1104
CviQI	GTAC	1 cut(s)	650
DdeI	CTNAG	6 cut(s)	113, 342, 441, 510, 792, 801
DpnI	GATC	8 cut(s)	254, 420, 439, 591, 726, 811, 927, 1011
DpnII	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
EciI	GGCGGA	1 cut(s)	62
Eco130I	CCWWGG	1 cut(s)	387
Eco147I	AGGCCT	3 cut(s)	101, 340, 665
Eco31I	GGTCTC	1 cut(s)	935
Eco47I	GGWCC	1 cut(s)	832
Eco57I	CTGAAG	2 cut(s)	507, 848
Eco72I	CACGTG	1 cut(s)	323
Eco88I	CYCGRG	1 cut(s)	633
EcoRII	CCWGG	1 cut(s)	335
EcoT14I	CCWWGG	1 cut(s)	387
ErhI	CCWWGG	1 cut(s)	387
Esp3I	CGTCTC	1 cut(s)	151
FaeI	CATG	2 cut(s)	662, 1107
FaiI	YATR	9 cut(s)	251, 453, 611, 660, 876, 1088, 1096, 1098, 1105
FalI	AAGNNNNNCTT	2 cut(s)	852, 884
FaqI	GGGAC	1 cut(s)	650
FatI	CATG	2 cut(s)	658, 1103
FauNDI	CATATG	1 cut(s)	1096
FbaI	TGATCA	1 cut(s)	925
Fnu4HI	GCNGC	7 cut(s)	54, 110, 519, 656, 681, 720, 1037
FokI	GGATG	2 cut(s)	174, 1091
Fsp4HI	GCNGC	7 cut(s)	54, 110, 519, 656, 681, 720, 1037
FspBI	CTAG	2 cut(s)	192, 845
GlaI	GCGC	1 cut(s)	1040
GluI	GCNGC	7 cut(s)	54, 110, 519, 656, 681, 720, 1037
GsuI	CTGGAG	1 cut(s)	38
HaeIII	GGCC	5 cut(s)	101, 222, 340, 665, 991
HapII	CCGG	4 cut(s)	459, 786, 853, 1069
HgaI	GACGC	3 cut(s)	38, 59, 155
HhaI	GCGC	1 cut(s)	1041
Hin1I	GRCGYC	2 cut(s)	30, 51
Hin1II	CATG	2 cut(s)	662, 1107
Hin6I	GCGC	1 cut(s)	1039
HinP1I	GCGC	1 cut(s)	1039
HincII	GTYRAC	2 cut(s)	603, 906
HindII	GTYRAC	2 cut(s)	603, 906
HindIII	AAGCTT	1 cut(s)	950
HinfI	GANTC	4 cut(s)	75, 299, 314, 752
HpaI	GTTAAC	1 cut(s)	603
HpaII	CCGG	4 cut(s)	459, 786, 853, 1069
HphI	GGTGA	2 cut(s)	701, 724
Hpy166II	GTNNAC	5 cut(s)	283, 603, 898, 906, 1111
Hpy188I	TCNGA	5 cut(s)	354, 442, 589, 795, 930
Hpy188III	TCNNGA	7 cut(s)	17, 115, 422, 459, 505, 635, 853
Hpy8I	GTNNAC	5 cut(s)	283, 603, 898, 906, 1111
Hpy99I	CGWCG	2 cut(s)	32, 71
HpyAV	CCTTC	8 cut(s)	112, 340, 364, 373, 406, 475, 790, 823
HpyCH4III	ACNGT	3 cut(s)	535, 748, 1124
HpyCH4IV	ACGT	2 cut(s)	310, 322
HpyCH4V	TGCA	4 cut(s)	658, 719, 1063, 1116
HpyF10VI	GCNNNNNNNGC	4 cut(s)	53, 524, 917, 988
HpyF3I	CTNAG	6 cut(s)	113, 342, 441, 510, 792, 801
HpySE526I	ACGT	2 cut(s)	310, 322
Hsp92I	GRCGYC	2 cut(s)	30, 51
Hsp92II	CATG	2 cut(s)	662, 1107
HspAI	GCGC	1 cut(s)	1039
Kpn2I	TCCGGA	2 cut(s)	458, 852
Ksp22I	TGATCA	1 cut(s)	925
KspAI	GTTAAC	1 cut(s)	603
Kzo9I	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
LmnI	GCTCC	2 cut(s)	10, 461
Lsp1109I	GCAGC	6 cut(s)	121, 505, 642, 667, 731, 1048
LweI	GCATC	1 cut(s)	196
MaeI	CTAG	2 cut(s)	192, 845
MaeII	ACGT	2 cut(s)	310, 322
MaeIII	GTNAC	3 cut(s)	127, 742, 1099
MalI	GATC	8 cut(s)	254, 420, 439, 591, 726, 811, 927, 1011
MboI	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
MboII	GAAGA	6 cut(s)	49, 52, 161, 367, 577, 968
MflI	RGATCY	1 cut(s)	437
MhlI	GDGCHC	1 cut(s)	762
MluCI	AATT	3 cut(s)	118, 769, 1013
MlyI	GAGTC	1 cut(s)	293
MmeI	TCCRAC	2 cut(s)	207, 1106
MroI	TCCGGA	2 cut(s)	458, 852
MseI	TTAA	4 cut(s)	408, 537, 602, 615
MspA1I	CMGCKG	3 cut(s)	527, 722, 911
MspI	CCGG	4 cut(s)	459, 786, 853, 1069
MspR9I	CCNGG	2 cut(s)	337, 1069
Mva1269I	GAATGC	2 cut(s)	451, 721
MvaI	CCWGG	1 cut(s)	337
MwoI	GCNNNNNNNGC	4 cut(s)	53, 524, 917, 988
NciI	CCSGG	1 cut(s)	1069
NdeI	CATATG	1 cut(s)	1096
NdeII	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
NlaIII	CATG	2 cut(s)	662, 1107
NlaIV	GGNNCC	3 cut(s)	457, 486, 781
NmuCI	GTSAC	2 cut(s)	127, 742
NspI	RCATGY	1 cut(s)	1107
PceI	AGGCCT	3 cut(s)	101, 340, 665
PciI	ACATGT	1 cut(s)	1103
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	2 cut(s)	451, 721
PfeI	GAWTC	3 cut(s)	75, 314, 752
PkrI	GCNGC	7 cut(s)	55, 111, 520, 657, 682, 721, 1038
PleI	GAGTC	1 cut(s)	293
PmaCI	CACGTG	1 cut(s)	323
PmlI	CACGTG	1 cut(s)	323
PpsI	GAGTC	1 cut(s)	293
Ppu21I	YACGTR	1 cut(s)	323
PscI	ACATGT	1 cut(s)	1103
Psp6I	CCWGG	1 cut(s)	335
PspCI	CACGTG	1 cut(s)	323
PspGI	CCWGG	1 cut(s)	335
PspN4I	GGNNCC	3 cut(s)	457, 486, 781
PspPI	GGNCC	2 cut(s)	832, 989
PsuI	RGATCY	1 cut(s)	437
PvuII	CAGCTG	1 cut(s)	722
RsaI	GTAC	1 cut(s)	651
RsaNI	GTAC	1 cut(s)	650
SaqAI	TTAA	4 cut(s)	408, 537, 602, 615
SatI	GCNGC	7 cut(s)	54, 110, 519, 656, 681, 720, 1037
Sau3AI	GATC	8 cut(s)	252, 418, 437, 589, 724, 809, 925, 1009
Sau96I	GGNCC	2 cut(s)	832, 989
SchI	GAGTC	1 cut(s)	293
ScrFI	CCNGG	2 cut(s)	337, 1069
SduI	GDGCHC	1 cut(s)	762
SfaNI	GCATC	1 cut(s)	196
SinI	GGWCC	1 cut(s)	832
SmlI	CTYRAG	1 cut(s)	95
SmoI	CTYRAG	1 cut(s)	95
Sse9I	AATT	3 cut(s)	118, 769, 1013
SseBI	AGGCCT	3 cut(s)	101, 340, 665
SsiI	CCGC	6 cut(s)	47, 54, 527, 683, 911, 1076
SspMI	CTAG	2 cut(s)	192, 845
StuI	AGGCCT	3 cut(s)	101, 340, 665
StyD4I	CCNGG	2 cut(s)	335, 1067
StyI	CCWWGG	1 cut(s)	387
TaaI	ACNGT	3 cut(s)	535, 748, 1124
TaiI	ACGT	2 cut(s)	313, 325
TaqI	TCGA	7 cut(s)	27, 78, 105, 172, 255, 733, 808
TasI	AATT	3 cut(s)	118, 769, 1013
TauI	GCSGC	1 cut(s)	56
TfiI	GAWTC	3 cut(s)	75, 314, 752
Tru1I	TTAA	4 cut(s)	408, 537, 602, 615
Tru9I	TTAA	4 cut(s)	408, 537, 602, 615
TscAI	CASTG	1 cut(s)	751
TseFI	GTSAC	2 cut(s)	127, 742
TseI	GCWGC	6 cut(s)	109, 518, 655, 680, 719, 1036
Tsp45I	GTSAC	2 cut(s)	127, 742
TspDTI	ATGAA	2 cut(s)	708, 1095
TspGWI	ACGGA	1 cut(s)	84
TspRI	CASTG	1 cut(s)	751
VpaK11BI	GGWCC	1 cut(s)	832
XapI	RAATTY	1 cut(s)	118
XceI	RCATGY	1 cut(s)	1107
XspI	CTAG	2 cut(s)	192, 845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.