Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0011422

Physical Location & Seq

Genomic Coordinates Reverse (-)

6412180 .. 6415339

Gene Structure 3160 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00284880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGAGAAGGAGCTGTTGGAGGTGTTTGACGACGTGACGAAGGCGGCCGACGCCGCGAAATCGTGCGACGGTGGCGCCGAGGAAAGTCTCTGTCTTGAGGCCTTGGATCGCCTTAACAATTTTCCCGTCTCTTATCAGCTTCTCGTCTCAACCCAAGTTGGGAAGCGTCTTAAGAATCTCACAAAACATCCTAGAAAGAAAATCCAGACTTTTGCGCTTAGCCTGATGGAATCATGGAAGCAAATTGTCATTGAGGAAACATCAAAAAATGATGTGAAGAATGGCAGGTATGAAAGAAAAGATTCGCTAAAGCGTGAGTCAAATCCCAATAGTCCCAGGCCTGTGAAGATTCAGAAAACTGCTGCTGACAAGGTTGAGAAGGCCCCCAAGGCTGAACAGGGTGACATTAAGAAGGTTGATCGTGATGTTAAACCAAGTTCAGAGAAAGCATATAGTTCTGAAAGTGGGACAACGGATAGTAAGGTTGAAAATCATAATGTTGTCAAGACTGAGAAAGCTCCCTTGGCGGATAATGTCAAGGTTGAAAAAGTAGCCAGAGAAGTGAAGAAACCTGTGTCTGGTCCCCCAAAGTTGACATCTATACCAAAGTCTAATGATCAGGCAAGGGACAGAGTAAGGGGAATGCTGCATGAGGCTTTGTCCAAGGTTTCCCAGGAGTCTGAGGCTAATGAAAGATTTGTGGATTCAGTGAATGCATGCGACCCTATTAGAGTTGCTGTTACAGTGGAGTCTGTGCTGTTTGAGAACTGGGGTGGTTCCAATGGGTCCCAGAAGGCTAAGTACCGATCACTAATTTTTAACCTGAAGGACCAAAAGAACCCAGATTTTCGCAGAAAAGTTCTTCTTGGAGATATCAAGGCAGAGAGGCTTGTAGACATGTCCACAGCTGAAATGGCAAGTGATGAGAGGCAATCAGAGAATAAAAGCTCGAAGAAAAGGCATTGTTTGAGTGTGAGCGTGGAGGTGCACCAAAAGCCACGACTGATCAATTCAAATGTGGTCGATGTGGGAAACGCAAGACCACCTACTACCAGATGCAGACACGGAGTGCCGATGAACCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med26 TFIIS_C TFIIS_M

Protein Analysis

361

Amino Acids

40.33

Weight (kDa)

9.35

Isoelectric Point (pI)

34.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med26	PF08711	33 - 83	4.3e-11	TFIIS helical bundle-like domain
TFIIS_M	PF07500	205 - 320	2.4e-32	Transcription factor S-II (TFIIS), central domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38560
fragaria_vesca	FvH4_7g26060 FvH4_7g26060 FvH4_7g26060
malus_domestica	MD01G1169100.v1.1 MD07G1235000.v1.1
prunus_persica	Prupe.2G265300_v2.0.a1
pyrus_communis	pycom01g18180 pycom07g21250
rosa_chinensis	RchiOBHm_Chr1g0372471
rosa_laevigata	RLG00000026896
rosa_multiflora	Rmu_sc0005410.1_g000001 Rmu_sc0011709.1_g000008
rosa_roxburghii	Rroxscaffold_4G00284880
rosa_rugosa	Rorug01G0372200
rosa_samantha	Rh1AG380700 Rh1BG344300 Rh1CG357700 Rh1DG375500
rosa_wichuraiana	Rw1G033490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	276
AccB1I	GGYRCC	1 cut(s)	74
AccI	GTMKAC	1 cut(s)	894
AccII	CGCG	1 cut(s)	56
AciI	CCGC	3 cut(s)	44, 54, 527
AclWI	GGATC	1 cut(s)	114
AcoI	YGGCCR	1 cut(s)	45
AcuI	CTGAAG	1 cut(s)	845
AcyI	GRCGYC	2 cut(s)	51, 75
AdeI	CACNNNGTG	1 cut(s)	1069
AfaI	GTAC	1 cut(s)	803
AfiI	CCNNNNNNNGG	2 cut(s)	159, 578
AflII	CTTAAG	1 cut(s)	170
AflIII	ACRYGT	1 cut(s)	897
AgsI	TTSAA	3 cut(s)	488, 545, 1014
AjiI	CACGTC	1 cut(s)	34
AjnI	CCWGG	2 cut(s)	335, 672
AjuI	GAANNNNNNNTTGG	3 cut(s)	31, 506, 538
AluBI	AGCT	5 cut(s)	13, 139, 518, 908, 948
AluI	AGCT	5 cut(s)	13, 139, 518, 908, 948
Alw21I	GWGCWC	1 cut(s)	990
Alw26I	GTCTC	3 cut(s)	92, 133, 151
Alw44I	GTGCAC	1 cut(s)	986
AlwI	GGATC	1 cut(s)	114
AoxI	GGCC	4 cut(s)	45, 99, 338, 381
ApaLI	GTGCAC	1 cut(s)	986
ApeKI	GCWGC	2 cut(s)	362, 646
Asp700I	GAANNNNTTC	1 cut(s)	301
AspLEI	GCGC	2 cut(s)	77, 217
AspS9I	GGNCC	4 cut(s)	382, 581, 786, 829
AsuHPI	GGTGA	1 cut(s)	413
AvaII	GGWCC	3 cut(s)	581, 786, 829
BaeGI	GKGCMC	1 cut(s)	990
BanI	GGYRCC	1 cut(s)	74
BarI	GAAGNNNNNNTAC	2 cut(s)	785, 817
Bbv12I	GWGCWC	1 cut(s)	990
BbvI	GCAGC	2 cut(s)	349, 633
BccI	CCATC	1 cut(s)	220
BciT130I	CCWGG	2 cut(s)	337, 674
BclI	TGATCA	2 cut(s)	616, 1005
BcoDI	GTCTC	3 cut(s)	92, 133, 151
BfaI	CTAG	1 cut(s)	192
BfoI	RGCGCY	1 cut(s)	78
BfrI	CTTAAG	1 cut(s)	170
BfuAI	ACCTGC	1 cut(s)	276
BglI	GCCNNNNNGGC	1 cut(s)	389
BisI	GCNGC	4 cut(s)	45, 54, 363, 647
BlpI	GCTNAGC	1 cut(s)	218
BlsI	GCNGC	4 cut(s)	46, 55, 364, 648
Bme1390I	CCNGG	2 cut(s)	337, 674
Bme18I	GGWCC	3 cut(s)	581, 786, 829
BmgBI	CACGTC	1 cut(s)	34
BmgT120I	GGNCC	4 cut(s)	382, 581, 786, 829
BmiI	GGNNCC	6 cut(s)	76, 384, 583, 778, 787, 788
BmrFI	CCNGG	2 cut(s)	337, 674
BmrI	ACTGGG	1 cut(s)	778
BmsI	GCATC	1 cut(s)	1046
BmuI	ACTGGG	1 cut(s)	778
Bpu1102I	GCTNAGC	1 cut(s)	218
BpuEI	CTTGAG	1 cut(s)	116
BsaHI	GRCGYC	2 cut(s)	51, 75
BsaJI	CCNNGG	7 cut(s)	78, 102, 335, 387, 522, 663, 672
Bsc4I	CCNNNNNNNGG	2 cut(s)	159, 578
Bse1I	ACTGG	1 cut(s)	773
BseBI	CCWGG	2 cut(s)	337, 674
BseDI	CCNNGG	7 cut(s)	78, 102, 335, 387, 522, 663, 672
BseGI	GGATG	1 cut(s)	187
BseLI	CCNNNNNNNGG	2 cut(s)	159, 578
BseMII	CTCAG	2 cut(s)	501, 672
BseNI	ACTGG	1 cut(s)	773
BseSI	GKGCMC	1 cut(s)	990
BseX3I	CGGCCG	1 cut(s)	45
BseXI	GCAGC	2 cut(s)	349, 633
Bsh1236I	CGCG	1 cut(s)	56
Bsh1285I	CGRYCG	1 cut(s)	48
BshFI	GGCC	4 cut(s)	47, 101, 340, 383
BshNI	GGYRCC	1 cut(s)	74
BsiEI	CGRYCG	1 cut(s)	48
BsiHKAI	GWGCWC	1 cut(s)	990
BslFI	GGGAC	5 cut(s)	318, 481, 567, 641, 772
BslI	CCNNNNNNNGG	2 cut(s)	159, 578
BsmAI	GTCTC	3 cut(s)	92, 133, 151
BsmBI	CGTCTC	2 cut(s)	133, 151
BsmFI	GGGAC	5 cut(s)	318, 481, 567, 641, 772
BsmI	GAATGC	2 cut(s)	648, 718
BsnI	GGCC	4 cut(s)	47, 101, 340, 383
Bsp1286I	GDGCHC	1 cut(s)	990
Bsp143I	GATC	5 cut(s)	106, 418, 616, 806, 1005
Bsp1720I	GCTNAGC	1 cut(s)	218
BspACI	CCGC	3 cut(s)	44, 54, 527
BspANI	GGCC	4 cut(s)	47, 101, 340, 383
BspCNI	CTCAG	2 cut(s)	502, 673
BspFNI	CGCG	1 cut(s)	56
BspLI	GGNNCC	6 cut(s)	76, 384, 583, 778, 787, 788
BspMI	ACCTGC	1 cut(s)	276
BspPI	GGATC	1 cut(s)	114
BspT107I	GGYRCC	1 cut(s)	74
BspTI	CTTAAG	1 cut(s)	170
BsrI	ACTGG	1 cut(s)	773
BssECI	CCNNGG	7 cut(s)	78, 102, 335, 387, 522, 663, 672
BssMI	GATC	5 cut(s)	106, 418, 616, 806, 1005
BssNI	GRCGYC	2 cut(s)	51, 75
BssT1I	CCWWGG	4 cut(s)	102, 387, 522, 663
Bst2UI	CCWGG	2 cut(s)	337, 674
Bst4CI	ACNGT	2 cut(s)	71, 745
BstACI	GRCGYC	2 cut(s)	51, 75
BstAFI	CTTAAG	1 cut(s)	170
BstC8I	GCNNGC	1 cut(s)	718
BstDEI	CTNAG	4 cut(s)	218, 510, 681, 798
BstF5I	GGATG	1 cut(s)	187
BstFNI	CGCG	1 cut(s)	56
BstH2I	RGCGCY	1 cut(s)	78
BstHHI	GCGC	2 cut(s)	77, 217
BstKTI	GATC	5 cut(s)	109, 421, 619, 809, 1008
BstMAI	GTCTC	3 cut(s)	92, 133, 151
BstMBI	GATC	5 cut(s)	106, 418, 616, 806, 1005
BstMCI	CGRYCG	1 cut(s)	48
BstMWI	GCNNNNNNNGC	7 cut(s)	50, 53, 72, 389, 524, 914, 994
BstNI	CCWGG	2 cut(s)	337, 674
BstNSI	RCATGY	2 cut(s)	720, 901
BstSCI	CCNGG	2 cut(s)	335, 672
BstSLI	GKGCMC	1 cut(s)	990
BstUI	CGCG	1 cut(s)	56
BstV1I	GCAGC	2 cut(s)	349, 633
BstZI	CGGCCG	1 cut(s)	45
BsuRI	GGCC	4 cut(s)	47, 101, 340, 383
BtrI	CACGTC	1 cut(s)	34
BtsCI	GGATG	1 cut(s)	187
BtsIMutI	CAGTG	2 cut(s)	714, 750
BveI	ACCTGC	1 cut(s)	276
Cac8I	GCNNGC	1 cut(s)	718
CfoI	GCGC	2 cut(s)	77, 217
Cfr13I	GGNCC	4 cut(s)	382, 581, 786, 829
CseI	GACGC	2 cut(s)	59, 155
Csp6I	GTAC	1 cut(s)	802
CviAII	CATG	4 cut(s)	234, 650, 717, 898
CviQI	GTAC	1 cut(s)	802
DdeI	CTNAG	4 cut(s)	218, 510, 681, 798
DinI	GGCGCC	1 cut(s)	76
DpnI	GATC	5 cut(s)	108, 420, 618, 808, 1007
DpnII	GATC	5 cut(s)	106, 418, 616, 806, 1005
DraIII	CACNNNGTG	1 cut(s)	1069
EaeI	YGGCCR	1 cut(s)	45
EagI	CGGCCG	1 cut(s)	45
EciI	GGCGGA	1 cut(s)	542
EclXI	CGGCCG	1 cut(s)	45
Eco130I	CCWWGG	4 cut(s)	102, 387, 522, 663
Eco147I	AGGCCT	2 cut(s)	101, 340
Eco32I	GATATC	1 cut(s)	874
Eco47I	GGWCC	3 cut(s)	581, 786, 829
Eco52I	CGGCCG	1 cut(s)	45
Eco57I	CTGAAG	1 cut(s)	845
EcoO109I	RGGNCCY	2 cut(s)	382, 786
EcoRII	CCWGG	2 cut(s)	335, 672
EcoRV	GATATC	1 cut(s)	874
EcoT14I	CCWWGG	4 cut(s)	102, 387, 522, 663
EcoT22I	ATGCAT	1 cut(s)	718
EgeI	GGCGCC	1 cut(s)	76
EheI	GGCGCC	1 cut(s)	76
ErhI	CCWWGG	4 cut(s)	102, 387, 522, 663
Esp3I	CGTCTC	2 cut(s)	133, 151
FaeI	CATG	4 cut(s)	237, 653, 720, 901
FalI	AAGNNNNNCTT	2 cut(s)	849, 881
FaqI	GGGAC	5 cut(s)	318, 481, 567, 641, 772
FatI	CATG	4 cut(s)	233, 649, 716, 897
FbaI	TGATCA	2 cut(s)	616, 1005
FblI	GTMKAC	1 cut(s)	894
Fnu4HI	GCNGC	4 cut(s)	45, 54, 363, 647
FokI	GGATG	1 cut(s)	174
Fsp4HI	GCNGC	4 cut(s)	45, 54, 363, 647
FspBI	CTAG	1 cut(s)	192
GlaI	GCGC	2 cut(s)	76, 216
GluI	GCNGC	4 cut(s)	45, 54, 363, 647
HaeII	RGCGCY	1 cut(s)	78
HaeIII	GGCC	4 cut(s)	47, 101, 340, 383
HgaI	GACGC	2 cut(s)	59, 155
HhaI	GCGC	2 cut(s)	77, 217
Hin1I	GRCGYC	2 cut(s)	51, 75
Hin1II	CATG	4 cut(s)	237, 653, 720, 901
Hin6I	GCGC	2 cut(s)	75, 215
HinP1I	GCGC	2 cut(s)	75, 215
HincII	GTYRAC	1 cut(s)	594
HindII	GTYRAC	1 cut(s)	594
HinfI	GANTC	8 cut(s)	175, 230, 302, 317, 349, 677, 704, 749
HphI	GGTGA	1 cut(s)	413
Hpy166II	GTNNAC	4 cut(s)	594, 895, 903, 988
Hpy188I	TCNGA	5 cut(s)	354, 442, 460, 682, 937
Hpy188III	TCNNGA	4 cut(s)	95, 205, 422, 505
Hpy8I	GTNNAC	4 cut(s)	594, 895, 903, 988
Hpy99I	CGWCG	3 cut(s)	35, 53, 71
HpyAV	CCTTC	5 cut(s)	34, 373, 406, 787, 820
HpyCH4III	ACNGT	2 cut(s)	71, 745
HpyCH4IV	ACGT	1 cut(s)	33
HpyCH4V	TGCA	4 cut(s)	649, 716, 988, 1059
HpyF10VI	GCNNNNNNNGC	7 cut(s)	50, 53, 72, 389, 524, 914, 994
HpyF3I	CTNAG	4 cut(s)	218, 510, 681, 798
HpySE526I	ACGT	1 cut(s)	33
Hsp92I	GRCGYC	2 cut(s)	51, 75
Hsp92II	CATG	4 cut(s)	237, 653, 720, 901
HspAI	GCGC	2 cut(s)	75, 215
KasI	GGCGCC	1 cut(s)	74
KflI	GGGWCCC	1 cut(s)	786
Ksp22I	TGATCA	2 cut(s)	616, 1005
Kzo9I	GATC	5 cut(s)	106, 418, 616, 806, 1005
LmnI	GCTCC	2 cut(s)	10, 523
Lsp1109I	GCAGC	2 cut(s)	349, 633
LweI	GCATC	1 cut(s)	1046
MaeI	CTAG	1 cut(s)	192
MaeII	ACGT	1 cut(s)	33
MaeIII	GTNAC	3 cut(s)	34, 401, 739
MalI	GATC	5 cut(s)	108, 420, 618, 808, 1007
MboI	GATC	5 cut(s)	106, 418, 616, 806, 1005
MboII	GAAGA	5 cut(s)	289, 358, 577, 854, 964
MhlI	GDGCHC	1 cut(s)	990
MluCI	AATT	4 cut(s)	118, 243, 813, 1009
Mly113I	GGCGCC	1 cut(s)	75
MlyI	GAGTC	3 cut(s)	326, 686, 758
MnlI	CCTC	9 cut(s)	13, 73, 91, 247, 646, 676, 879, 921, 976
Mph1103I	ATGCAT	1 cut(s)	718
MroXI	GAANNNNTTC	1 cut(s)	301
MseI	TTAA	5 cut(s)	114, 171, 408, 429, 819
MspA1I	CMGCKG	1 cut(s)	908
MspCI	CTTAAG	1 cut(s)	170
MspR9I	CCNGG	2 cut(s)	337, 674
Mva1269I	GAATGC	2 cut(s)	648, 718
MvaI	CCWGG	2 cut(s)	337, 674
MvnI	CGCG	1 cut(s)	56
MwoI	GCNNNNNNNGC	7 cut(s)	50, 53, 72, 389, 524, 914, 994
NarI	GGCGCC	1 cut(s)	75
NdeII	GATC	5 cut(s)	106, 418, 616, 806, 1005
NlaIII	CATG	4 cut(s)	237, 653, 720, 901
NlaIV	GGNNCC	6 cut(s)	76, 384, 583, 778, 787, 788
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	2 cut(s)	34, 401
NsiI	ATGCAT	1 cut(s)	718
NspI	RCATGY	2 cut(s)	720, 901
PaeI	GCATGC	1 cut(s)	720
PceI	AGGCCT	2 cut(s)	101, 340
PciI	ACATGT	1 cut(s)	897
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	2 cut(s)	648, 718
PdmI	GAANNNNTTC	1 cut(s)	301
PfeI	GAWTC	5 cut(s)	175, 230, 302, 349, 704
PkrI	GCNGC	4 cut(s)	46, 55, 364, 648
PleI	GAGTC	3 cut(s)	325, 685, 757
PluTI	GGCGCC	1 cut(s)	78
PpsI	GAGTC	3 cut(s)	325, 685, 757
PpuMI	RGGWCCY	1 cut(s)	786
PscI	ACATGT	1 cut(s)	897
Psp5II	RGGWCCY	1 cut(s)	786
Psp6I	CCWGG	2 cut(s)	335, 672
PspGI	CCWGG	2 cut(s)	335, 672
PspN4I	GGNNCC	6 cut(s)	76, 384, 583, 778, 787, 788
PspPI	GGNCC	4 cut(s)	382, 581, 786, 829
PspPPI	RGGWCCY	1 cut(s)	786
PvuII	CAGCTG	1 cut(s)	908
RsaI	GTAC	1 cut(s)	803
RsaNI	GTAC	1 cut(s)	802
SaqAI	TTAA	5 cut(s)	114, 171, 408, 429, 819
SatI	GCNGC	4 cut(s)	45, 54, 363, 647
Sau3AI	GATC	5 cut(s)	106, 418, 616, 806, 1005
Sau96I	GGNCC	4 cut(s)	382, 581, 786, 829
SchI	GAGTC	3 cut(s)	326, 686, 758
ScrFI	CCNGG	2 cut(s)	337, 674
SduI	GDGCHC	1 cut(s)	990
SfaNI	GCATC	1 cut(s)	1046
SfoI	GGCGCC	1 cut(s)	76
SinI	GGWCC	3 cut(s)	581, 786, 829
SmlI	CTYRAG	2 cut(s)	95, 170
SmoI	CTYRAG	2 cut(s)	95, 170
SphI	GCATGC	1 cut(s)	720
Sse9I	AATT	4 cut(s)	118, 243, 813, 1009
SseBI	AGGCCT	2 cut(s)	101, 340
SsiI	CCGC	3 cut(s)	44, 54, 527
SspDI	GGCGCC	1 cut(s)	74
SspMI	CTAG	1 cut(s)	192
StuI	AGGCCT	2 cut(s)	101, 340
StyD4I	CCNGG	2 cut(s)	335, 672
StyI	CCWWGG	4 cut(s)	102, 387, 522, 663
TaaI	ACNGT	2 cut(s)	71, 745
TaiI	ACGT	1 cut(s)	36
TaqI	TCGA	2 cut(s)	950, 1023
TasI	AATT	4 cut(s)	118, 243, 813, 1009
TauI	GCSGC	2 cut(s)	47, 56
TfiI	GAWTC	5 cut(s)	175, 230, 302, 349, 704
Tru1I	TTAA	5 cut(s)	114, 171, 408, 429, 819
Tru9I	TTAA	5 cut(s)	114, 171, 408, 429, 819
TscAI	CASTG	2 cut(s)	714, 750
TseFI	GTSAC	2 cut(s)	34, 401
TseI	GCWGC	2 cut(s)	362, 646
Tsp45I	GTSAC	2 cut(s)	34, 401
TspDTI	ATGAA	2 cut(s)	306, 705
TspGWI	ACGGA	2 cut(s)	488, 1080
TspRI	CASTG	2 cut(s)	714, 750
Vha464I	CTTAAG	1 cut(s)	170
VneI	GTGCAC	1 cut(s)	986
VpaK11BI	GGWCC	3 cut(s)	581, 786, 829
XceI	RCATGY	2 cut(s)	720, 901
XcmI	CCANNNNNNNNNTGG	1 cut(s)	910
XmiI	GTMKAC	1 cut(s)	894
XmnI	GAANNNNTTC	1 cut(s)	301
XspI	CTAG	1 cut(s)	192
Zsp2I	ATGCAT	1 cut(s)	718

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.