MD01G1171200.v1.1

Hydroxyacylglutathione hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0017173
Physical Location & Seq
Forward (+)
27402171 .. 27404982
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1171200.v1.1.491

Sequence Viewer

Length: 777 bp
ATGAAAATCCATCACGTAGCTTGCTTGGAGGACAACTACTCGTACCTAATTGTTGACGAGAGTACAAAAGAAGCGGCGGTTGTGGATCCAGTTGAGCCTGAGAAGGTTCTGAAAGCAGCCCAAGAACACGGCGTCGATGTCAAGCTCGTCCTCACCACTCACCACCACTGGGATCACTCTGGTGGGAATGAAAAGATAAAGCAGTTGGTGCCAGGGATTAAGGTGTATGGCGGTTCTATTGATAATGTGAAGGGCTGCACCGATAAGGTAGAAAATGGTGATAAGATTTCGCTCGGGGCTGATGTTCACATATTGTGTCTTCACACACCTTGCCACACTAAAGGTCACATAAGTTATTATGTGACTAGCAAAGAGGGAGAGGACCCAGCTGTTTTTACTGGAGACACACTGTTCATTGCTGGTTGCGGGAAATTTTTCGAAGGAACCGCAGAGCAGATGCATCAATCGCTATGCGTAACTTTGGCTTCATTGCCAAAGCCAACTCAAGTTTACTGTGGCCACGAGTACACTGTGAAGAACTTGCAATTTGCTCAAACGGTTGAACCGGACAATGCGAAGATAAAGCAGAAGCTATCATGGGCACAGCAGCAGCGTGAAGCAGGCCTTCCCACAGTTCCTTCAACCATAGAGGAAGAGATGGAGACAAACCCTTTTATCCGTGCCGATCTACCAGAGCTTCAGGAAAGGGTCGGTTGCAAAACCGCCGTCGAAGCTCTTCGGGAGATAAGGCAGCGGAAGGACAATTGGAGGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
HAGH_C Lactamase_B

Protein Analysis

259

Amino Acids

28.68

Weight (kDa)

5.8

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 13 - 174 1.2e-12 Metallo-beta-lactamase superfamily
HAGH_C PF16123 175 - 256 1.9e-29 Hydroxyacylglutathione hydrolase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10850
fragaria_vesca FvH4_7g26250 FvH4_7g26250
malus_domestica MD01G1171200.v1.1
prunus_persica Prupe.2G266700_v2.0.a1
pyrus_communis pycom01g18340
rosa_chinensis RchiOBHm_Chr1g0372701
rosa_laevigata RLG00000026878
rosa_multiflora Rmu_sc0034231.1_g000002
rosa_roxburghii Rroxscaffold_4G00284740
rosa_rugosa Rorug01G0373800
rosa_samantha Rh1BG346000
rosa_wichuraiana Rw1G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 208
AciI CCGC 7 cut(s) 74, 77, 231, 426, 447, 723, 754
AclWI GGATC 3 cut(s) 80, 93, 180
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 1 cut(s) 431
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 3 cut(s) 44, 64, 527
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 563, 642
AjnI CCWGG 1 cut(s) 211
AleI CACNNNNGTG 1 cut(s) 180
AluBI AGCT 6 cut(s) 20, 145, 389, 592, 697, 734
AluI AGCT 6 cut(s) 20, 145, 389, 592, 697, 734
Alw26I GTCTC 2 cut(s) 396, 656
AlwI GGATC 3 cut(s) 80, 93, 180
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 2 cut(s) 517, 622
ApeKI GCWGC 5 cut(s) 116, 255, 607, 610, 751
ApoI RAATTY 1 cut(s) 431
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
Asp700I GAANNNNTTC 2 cut(s) 434, 735
AspS9I GGNCC 1 cut(s) 382
AsuHPI GGTGA 3 cut(s) 145, 152, 290
AsuII TTCGAA 1 cut(s) 438
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 382
BaeGI GKGCMC 1 cut(s) 604
BalI TGGCCA 1 cut(s) 519
BamHI GGATCC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 208
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 311
BbvI GCAGC 5 cut(s) 128, 242, 619, 622, 763
BccI CCATC 2 cut(s) 18, 652
BceAI ACGGC 2 cut(s) 145, 710
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 396, 656
BfaI CTAG 1 cut(s) 366
BisI GCNGC 6 cut(s) 75, 117, 256, 608, 611, 752
BlsI GCNGC 6 cut(s) 76, 118, 257, 609, 612, 753
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 382
BmiI GGNNCC 4 cut(s) 87, 210, 384, 445
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 447, 469
BmuI ACTGGG 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 311
BpmI CTGGAG 1 cut(s) 420
Bpu14I TTCGAA 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 489
BsaAI YACGTR 1 cut(s) 16
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 212
BsaWI WCCGGW 1 cut(s) 565
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 89, 173, 403
Bse3DI GCAATG 2 cut(s) 414, 488
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 2 cut(s) 414, 488
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 3 cut(s) 89, 173, 403
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 5 cut(s) 128, 242, 619, 622, 763
BseYI CCCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 241
BshFI GGCC 2 cut(s) 519, 624
BshNI GGYRCC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 2 cut(s) 396, 656
BsnI GGCC 2 cut(s) 519, 624
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 604
Bsp143I GATC 3 cut(s) 85, 172, 685
BspACI CCGC 7 cut(s) 74, 77, 231, 426, 447, 723, 754
BspANI GGCC 2 cut(s) 519, 624
BspCNI CTCAG 1 cut(s) 91
BspLI GGNNCC 4 cut(s) 87, 210, 384, 445
BspPI GGATC 3 cut(s) 80, 93, 180
BspQI GCTCTTC 1 cut(s) 741
BspT104I TTCGAA 1 cut(s) 438
BspT107I GGYRCC 1 cut(s) 208
BsrDI GCAATG 2 cut(s) 414, 488
BsrI ACTGG 3 cut(s) 89, 173, 403
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 3 cut(s) 85, 172, 685
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 521
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 5 cut(s) 411, 515, 532, 559, 634
Bst6I CTCTTC 2 cut(s) 648, 741
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 16
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 2 cut(s) 22, 622
BstDEI CTNAG 1 cut(s) 99
BstKTI GATC 3 cut(s) 88, 175, 688
BstMAI GTCTC 2 cut(s) 396, 656
BstMBI GATC 3 cut(s) 85, 172, 685
BstMWI GCNNNNNNNGC 3 cut(s) 208, 466, 731
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 5 cut(s) 128, 242, 619, 622, 763
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BsuRI GGCC 2 cut(s) 519, 624
BtsIMutI CAGTG 3 cut(s) 166, 407, 528
Cac8I GCNNGC 2 cut(s) 22, 622
Cfr13I GGNCC 1 cut(s) 382
CseI GACGC 1 cut(s) 121
Csp6I GTAC 3 cut(s) 43, 63, 526
CviAII CATG 1 cut(s) 597
CviQI GTAC 3 cut(s) 43, 63, 526
DdeI CTNAG 1 cut(s) 99
DpnI GATC 3 cut(s) 87, 174, 687
DpnII GATC 3 cut(s) 85, 172, 685
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 2 cut(s) 648, 741
EarI CTCTTC 2 cut(s) 648, 741
Eco147I AGGCCT 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 462
FaeI CATG 1 cut(s) 600
FaiI YATR 7 cut(s) 228, 311, 350, 360, 472, 598, 647
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 1 cut(s) 596
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 6 cut(s) 75, 117, 256, 608, 611, 752
Fsp4HI GCNGC 6 cut(s) 75, 117, 256, 608, 611, 752
FspBI CTAG 1 cut(s) 366
GluI GCNGC 6 cut(s) 75, 117, 256, 608, 611, 752
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 420
HaeIII GGCC 2 cut(s) 519, 624
HapII CCGG 1 cut(s) 566
HgaI GACGC 1 cut(s) 121
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 1 cut(s) 600
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HpaII CCGG 1 cut(s) 566
HphI GGTGA 3 cut(s) 145, 152, 290
Hpy166II GTNNAC 4 cut(s) 55, 307, 511, 528
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 2 cut(s) 701, 740
Hpy8I GTNNAC 4 cut(s) 55, 307, 511, 528
Hpy99I CGWCG 2 cut(s) 137, 731
HpyAV CCTTC 6 cut(s) 97, 244, 434, 635, 648, 751
HpyCH4III ACNGT 5 cut(s) 411, 515, 532, 559, 634
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 4 cut(s) 258, 460, 544, 717
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 466, 731
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 1 cut(s) 600
Kzo9I GATC 3 cut(s) 85, 172, 685
LguI GCTCTTC 1 cut(s) 741
Lsp1109I GCAGC 5 cut(s) 128, 242, 619, 622, 763
LweI GCATC 2 cut(s) 447, 469
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 3 cut(s) 344, 361, 475
MalI GATC 3 cut(s) 87, 174, 687
MboI GATC 3 cut(s) 85, 172, 685
MboII GAAGA 5 cut(s) 311, 547, 589, 665, 728
MfeI CAATTG 1 cut(s) 763
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 604
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 4 cut(s) 48, 431, 545, 763
MluNI TGGCCA 1 cut(s) 519
MnlI CCTC 6 cut(s) 22, 161, 367, 373, 643, 762
Mox20I TGGCCA 1 cut(s) 519
Mph1103I ATGCAT 1 cut(s) 462
MroXI GAANNNNTTC 2 cut(s) 434, 735
MscI TGGCCA 1 cut(s) 519
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 180
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 2 cut(s) 389, 754
MspI CCGG 1 cut(s) 566
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 763
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 208, 466, 731
NdeII GATC 3 cut(s) 85, 172, 685
NlaIII CATG 1 cut(s) 600
NlaIV GGNNCC 4 cut(s) 87, 210, 384, 445
NmuCI GTSAC 2 cut(s) 344, 361
NsiI ATGCAT 1 cut(s) 462
NspV TTCGAA 1 cut(s) 438
OliI CACNNNNGTG 1 cut(s) 180
PceI AGGCCT 1 cut(s) 624
PciSI GCTCTTC 1 cut(s) 741
PdmI GAANNNNTTC 2 cut(s) 434, 735
PkrI GCNGC 6 cut(s) 76, 118, 257, 609, 612, 753
Ppu21I YACGTR 1 cut(s) 16
PpuMI RGGWCCY 1 cut(s) 382
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 211
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 87, 210, 384, 445
PspPI GGNCC 1 cut(s) 382
PspPPI RGGWCCY 1 cut(s) 382
PsuI RGATCY 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 3 cut(s) 44, 64, 527
RsaNI GTAC 3 cut(s) 43, 63, 526
RseI CAYNNNNRTG 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 6 cut(s) 75, 117, 256, 608, 611, 752
Sau3AI GATC 3 cut(s) 85, 172, 685
Sau96I GGNCC 1 cut(s) 382
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 2 cut(s) 447, 469
SfuI TTCGAA 1 cut(s) 438
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
Sse9I AATT 4 cut(s) 48, 431, 545, 763
SseBI AGGCCT 1 cut(s) 624
SsiI CCGC 7 cut(s) 74, 77, 231, 426, 447, 723, 754
SspMI CTAG 1 cut(s) 366
StuI AGGCCT 1 cut(s) 624
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 5 cut(s) 411, 515, 532, 559, 634
TaiI ACGT 1 cut(s) 18
TaqI TCGA 3 cut(s) 135, 438, 729
TasI AATT 4 cut(s) 48, 431, 545, 763
TatI WGTACW 2 cut(s) 62, 525
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 3 cut(s) 173, 414, 535
TseFI GTSAC 2 cut(s) 344, 361
TseI GCWGC 5 cut(s) 116, 255, 607, 610, 751
Tsp45I GTSAC 2 cut(s) 344, 361
TspDTI ATGAA 4 cut(s) 17, 204, 403, 477
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 3 cut(s) 173, 414, 535
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 1 cut(s) 431
XmnI GAANNNNTTC 2 cut(s) 434, 735
XspI CTAG 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.