Rh1BG346000

Hydroxyacylglutathione hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0017173
Physical Location & Seq
Forward (+)
47872633 .. 47875630
2998 bp
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UTR
Exon/CDS
Intron
Rh1BG346000.1

Sequence Viewer

Length: 570 bp
ATGAAAATCCATCACGTTGCTTGCTTGGAAGACAATTACGCATACCTAATTATTGACGAGAGCACCAAAGAAGCCGCGGTTGTGGATCCTGTTGAACCCGAAAAGGTTCTTAAAGCAGCTCAAGACCACGGTGCCCAAGTCAAACTCGTCCTCACCACTCACCACCATTGGGATCATGCTGGTGGAAATGAGAAGATTAAGCAGTTGGTGCCTGGTATTAAAGTATATGGGGGTTCTGTTGATAATGTGAAGGGGTGCACTGATGAGGTGGACAATGGTGATAAGATTTCGCTCGGAGCTGATACTCAAATACTCTCGCTTCACACACCTTGTCACACAAAAGGTCATATAAGTTATTATGTGACTAGCAAAGAGGGAGAGGAACCAGCTGTTTTCACCGGAGACACACTGTTTATTGCTGGTTGCGGCAAATTTTTCGAAGGAACAGCTGAACAGATGCATCAGTCACTATGCGTTACTTTGGCTTCATTGCCAAAGCCTACACGAGTTTACTGTGGTCATGAGGTAAAAGAAGAGTCTGATACTTTTTTGCTTCTTTCCTTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
HAGH_C Lactamase_B

Protein Analysis

189

Amino Acids

20.59

Weight (kDa)

5.46

Isoelectric Point (pI)

25.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 13 - 174 1.2e-14 Metallo-beta-lactamase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10850
fragaria_vesca FvH4_7g26250 FvH4_7g26250
malus_domestica MD01G1171200.v1.1
prunus_persica Prupe.2G266700_v2.0.a1
pyrus_communis pycom01g18340
rosa_chinensis RchiOBHm_Chr1g0372701
rosa_laevigata RLG00000026878
rosa_multiflora Rmu_sc0034231.1_g000002
rosa_roxburghii Rroxscaffold_4G00284740
rosa_rugosa Rorug01G0373800
rosa_samantha Rh1BG346000
rosa_wichuraiana Rw1G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 131, 208
AccII CGCG 1 cut(s) 77
AciI CCGC 3 cut(s) 75, 77, 426
AclWI GGATC 3 cut(s) 80, 93, 180
AcsI RAATTY 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 1 cut(s) 95
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 4 cut(s) 119, 299, 389, 449
AluI AGCT 4 cut(s) 119, 299, 389, 449
Alw21I GWGCWC 2 cut(s) 65, 260
Alw26I GTCTC 1 cut(s) 396
Alw44I GTGCAC 1 cut(s) 256
AlwI GGATC 3 cut(s) 80, 93, 180
ApaLI GTGCAC 1 cut(s) 256
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 105
AsuHPI GGTGA 4 cut(s) 145, 152, 290, 388
AsuII TTCGAA 1 cut(s) 438
BaeGI GKGCMC 2 cut(s) 136, 260
BamHI GGATCC 1 cut(s) 85
BanI GGYRCC 2 cut(s) 131, 208
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 65, 260
BbvI GCAGC 1 cut(s) 128
BccI CCATC 1 cut(s) 18
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 396
BfaI CTAG 1 cut(s) 366
BisI GCNGC 3 cut(s) 75, 117, 427
BlsI GCNGC 3 cut(s) 76, 118, 428
Bme1390I CCNGG 1 cut(s) 213
BmiI GGNNCC 4 cut(s) 87, 133, 210, 384
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 2 cut(s) 447, 469
BpiI GAAGAC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 75, 127
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 488
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 75, 127
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 488
BseSI GKGCMC 2 cut(s) 136, 260
BseXI GCAGC 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 77
BshNI GGYRCC 2 cut(s) 131, 208
BsiHKAI GWGCWC 2 cut(s) 65, 260
BsiSI CCGG 1 cut(s) 399
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 1 cut(s) 396
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 3 cut(s) 65, 136, 260
Bsp143I GATC 2 cut(s) 85, 172
BspACI CCGC 3 cut(s) 75, 77, 426
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 4 cut(s) 87, 133, 210, 384
BspPI GGATC 3 cut(s) 80, 93, 180
BspT104I TTCGAA 1 cut(s) 438
BspT107I GGYRCC 2 cut(s) 131, 208
BsrDI GCAATG 1 cut(s) 488
BssECI CCNNGG 2 cut(s) 75, 127
BssMI GATC 2 cut(s) 85, 172
BssSI CACGAG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 3 cut(s) 131, 411, 515
Bst6I CTCTTC 1 cut(s) 528
BstAPI GCANNNNNTGC 1 cut(s) 208
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 22
BstDSI CCRYGG 2 cut(s) 75, 127
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 2 cut(s) 88, 175
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 2 cut(s) 85, 172
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 2 cut(s) 136, 260
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BtgI CCRYGG 2 cut(s) 75, 127
BtsIMutI CAGTG 2 cut(s) 258, 407
Cac8I GCNNGC 1 cut(s) 22
CciI TCATGA 1 cut(s) 520
Cfr42I CCGCGG 1 cut(s) 78
CviAII CATG 2 cut(s) 176, 521
CviJI RGCY 7 cut(s) 74, 119, 299, 389, 449, 485, 499
CviKI_1 RGCY 7 cut(s) 74, 119, 299, 389, 449, 485, 499
DpnI GATC 2 cut(s) 87, 174
DpnII GATC 2 cut(s) 85, 172
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 462
FaeI CATG 2 cut(s) 179, 524
FaiI YATR 9 cut(s) 43, 177, 226, 228, 348, 350, 360, 472, 522
FatI CATG 2 cut(s) 175, 520
Fnu4HI GCNGC 3 cut(s) 75, 117, 427
Fsp4HI GCNGC 3 cut(s) 75, 117, 427
FspBI CTAG 1 cut(s) 366
GluI GCNGC 3 cut(s) 75, 117, 427
HapII CCGG 1 cut(s) 399
Hin1II CATG 2 cut(s) 179, 524
HinfI GANTC 1 cut(s) 536
HpaII CCGG 1 cut(s) 399
HphI GGTGA 4 cut(s) 145, 152, 290, 388
Hpy166II GTNNAC 3 cut(s) 258, 271, 511
Hpy188I TCNGA 2 cut(s) 296, 541
Hpy188III TCNNGA 2 cut(s) 122, 521
Hpy8I GTNNAC 3 cut(s) 258, 271, 511
HpyAV CCTTC 2 cut(s) 244, 434
HpyCH4III ACNGT 3 cut(s) 131, 411, 515
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 258, 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 2 cut(s) 179, 524
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 2 cut(s) 85, 172
LmnI GCTCC 1 cut(s) 296
LpnPI CCDG 7 cut(s) 102, 165, 198, 225, 399, 405, 412
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 2 cut(s) 447, 469
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 4 cut(s) 332, 361, 465, 475
MalI GATC 2 cut(s) 87, 174
MboI GATC 2 cut(s) 85, 172
MboII GAAGA 3 cut(s) 41, 205, 545
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 3 cut(s) 65, 136, 260
MluCI AATT 3 cut(s) 34, 48, 431
MlyI GAGTC 1 cut(s) 545
MnlI CCTC 5 cut(s) 161, 259, 367, 373, 517
Mph1103I ATGCAT 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 3 cut(s) 111, 198, 219
MslI CAYNNNNRTG 1 cut(s) 180
MspA1I CMGCKG 3 cut(s) 77, 389, 449
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 208
NdeII GATC 2 cut(s) 85, 172
NlaIII CATG 2 cut(s) 179, 524
NlaIV GGNNCC 4 cut(s) 87, 133, 210, 384
NmuCI GTSAC 3 cut(s) 332, 361, 465
NsiI ATGCAT 1 cut(s) 462
NspV TTCGAA 1 cut(s) 438
PagI TCATGA 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 105
PkrI GCNGC 3 cut(s) 76, 118, 428
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 87, 133, 210, 384
PsuI RGATCY 1 cut(s) 85
PvuII CAGCTG 2 cut(s) 389, 449
RseI CAYNNNNRTG 1 cut(s) 180
SacII CCGCGG 1 cut(s) 78
SaqAI TTAA 3 cut(s) 111, 198, 219
SatI GCNGC 3 cut(s) 75, 117, 427
Sau3AI GATC 2 cut(s) 85, 172
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 3 cut(s) 65, 136, 260
SfaNI GCATC 2 cut(s) 447, 469
Sfr303I CCGCGG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 438
SgrBI CCGCGG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
Sse9I AATT 3 cut(s) 34, 48, 431
SsiI CCGC 3 cut(s) 75, 77, 426
SspMI CTAG 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 3 cut(s) 131, 411, 515
TaiI ACGT 1 cut(s) 18
TaqI TCGA 1 cut(s) 438
TasI AATT 3 cut(s) 34, 48, 431
TauI GCSGC 2 cut(s) 77, 429
Tru1I TTAA 3 cut(s) 111, 198, 219
Tru9I TTAA 3 cut(s) 111, 198, 219
TscAI CASTG 2 cut(s) 265, 414
TseFI GTSAC 3 cut(s) 332, 361, 465
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 3 cut(s) 332, 361, 465
TspDTI ATGAA 2 cut(s) 17, 477
TspRI CASTG 2 cut(s) 265, 414
VneI GTGCAC 1 cut(s) 256
XapI RAATTY 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.