Rorug01G0373800

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003864
Physical Location & Seq
Forward (+)
48711684 .. 48716038
4355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0373800.1

Sequence Viewer

Length: 1509 bp
ATGCTCACAGAACAAGAAGCACAAAAACTGGCTGAAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAAAAGAAGAGCGTCAAAACAACAAAGTCATGGAACTTCCTTGGGTTTCCAGAAACAGTTAAGAGAAGCCCCCTTGAAAAAGATATCATCGTTGGTGTGATTGACACTGGAATTTGGCCAGAGTCGCACAGCTTTAGTGATGTTGGATTCGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAAGCAATGGCAACTTTACTTGTAACAATAAAATCATCGGAGCACGGTATTACCATCATTCTGGATCCTTTGAAGAAGGAGAATTTGAGTCTCCAAGAGACTCAAAGGGTCATGGAACCCACACTGCATCAACAGCAGCTGGGAACATCGTGAGAGAAGCGAGCCTAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGGGGTGCCATCAGCACATATTGCAGTGTACAAAGCATGTTGGGAAGATGGCTGTGATGACGCTGACGTTCTAGCAGCATTTGATGATGCTGTTTCTGACGGTGTTGACATTCTCTCTGCTTCTGCCGGGAACCAGATTCCACATGATTATTTGGAGGATGGATTTGCAATTGGGTCATTTCACGCTACCATAAATGGGATATTGGTTTCGATGTCCGCTGGTAACGAAGGTCCGAAAAAAAAAACTGTGGGAAATTATGCACCATGGCAACTCTCTGTGGGTGCTGTCAACATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGGACTTTTACCAAACTCATATGACCTCCAGGGTAAATTCTATGATTTAATTTATGGTGGAGACGCACCCAATACAAAGCCAGGTGGTAATAGGATGTCCAGATATTGCAAGAAAGATAGCTTAGAAGAAGATTTGGTCAAAGGTAAAATTGTGCTTTGTGATAGAGGCCCCGATAAGATTGAGGATGGCGATTGGGATGGGTCTGGGGCCTTACAGGCTAATGCAACTGGAATTATTCTAACGGGCGTTAAAGTTGCTGAGGAAATTGTTGACCCTATTCCTCGTCCTGCATCTCACGTCGGATTGGAAGAAAATAGCAATATTTACATGTACATAAACAAAACAAGGAACCCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGGTGAATCCCAACATTCTTAAGCCAGATTTAGTGGCTACAGGAACTGAGATTCTAGCAGCATATGATCAACCAATTGGTGATGATGCTAACAATAGAGCTGAATACAATTTGGAGACTGGGACATCAATGGCGTGCCCCCATGCTACGGCCGTAGCTGCATACGTCAAATCATTTCACCCTGAATGGTCACCAGCTGCTATCAAATCGGCTCTCATGACTACCGCTACACCCATGACAAAACTAGCCCGCACGCTGAATTTGCATATGGAGCTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

502

Amino Acids

53.98

Weight (kDa)

5.46

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 49 - 487 1e-43 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 451
AccB7I CCANNNNNTGG 1 cut(s) 234
AciI CCGC 4 cut(s) 663, 745, 1452, 1477
AclWI GGATC 2 cut(s) 309, 322
AcoI YGGCCR 2 cut(s) 182, 1377
AcsI RAATTY 4 cut(s) 177, 332, 827, 1486
AfaI GTAC 2 cut(s) 476, 1124
AfiI CCNNNNNNNGG 3 cut(s) 234, 642, 1375
AflII CTTAAG 1 cut(s) 1247
AflIII ACRYGT 1 cut(s) 1119
AgsI TTSAA 3 cut(s) 143, 323, 767
AjiI CACGTC 1 cut(s) 1090
AjnI CCWGG 2 cut(s) 819, 871
AluBI AGCT 7 cut(s) 198, 389, 912, 1328, 1385, 1424, 1501
AluI AGCT 7 cut(s) 198, 389, 912, 1328, 1385, 1424, 1501
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 4 cut(s) 342, 345, 846, 1337
AlwI GGATC 2 cut(s) 309, 322
AlwNI CAGNNNCTG 2 cut(s) 389, 1274
AoxI GGCC 5 cut(s) 182, 958, 999, 1377, 1503
ApeKI GCWGC 5 cut(s) 386, 521, 1286, 1385, 1424
ApoI RAATTY 4 cut(s) 177, 332, 827, 1486
AseI ATTAAT 1 cut(s) 1176
AspS9I GGNCC 5 cut(s) 218, 677, 959, 999, 1220
AsuC2I CCSGG 1 cut(s) 574
AsuHPI GGTGA 5 cut(s) 748, 1243, 1319, 1397, 1410
AvaII GGWCC 3 cut(s) 218, 677, 1220
BaeGI GKGCMC 2 cut(s) 1203, 1367
BalI TGGCCA 1 cut(s) 184
BamHI GGATCC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 451
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 1050
BbvI GCAGC 5 cut(s) 398, 533, 1298, 1372, 1411
BccI CCATC 7 cut(s) 35, 312, 463, 488, 599, 971, 983
BceAI ACGGC 2 cut(s) 1364, 1392
BciT130I CCWGG 2 cut(s) 821, 873
BclI TGATCA 1 cut(s) 1294
BcnI CCSGG 1 cut(s) 574
BcoDI GTCTC 4 cut(s) 342, 345, 846, 1337
BfaI CTAG 3 cut(s) 518, 1283, 1472
BfmI CTRYAG 1 cut(s) 1266
BfrI CTTAAG 1 cut(s) 1247
BglI GCCNNNNNGGC 1 cut(s) 1007
BisI GCNGC 5 cut(s) 387, 522, 1287, 1386, 1425
BlsI GCNGC 5 cut(s) 388, 523, 1288, 1387, 1426
Bme1390I CCNGG 3 cut(s) 574, 821, 873
Bme18I GGWCC 3 cut(s) 218, 677, 1220
BmgBI CACGTC 1 cut(s) 1090
BmgT120I GGNCC 5 cut(s) 218, 677, 959, 999, 1220
BmiI GGNNCC 9 cut(s) 316, 367, 453, 578, 961, 1000, 1142, 1191, 1221
BmrFI CCNGG 3 cut(s) 574, 821, 873
BmrI ACTGGG 1 cut(s) 1356
BmsI GCATC 4 cut(s) 386, 523, 1091, 1303
BmuI ACTGGG 1 cut(s) 1356
BpiI GAAGAC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 1 cut(s) 1050
BpuMI CCSGG 1 cut(s) 574
BsaJI CCNNGG 4 cut(s) 106, 710, 759, 820
BsaWI WCCGGW 1 cut(s) 1228
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 642, 1375
Bse1I ACTGG 4 cut(s) 33, 178, 1024, 1351
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 2 cut(s) 821, 873
BseDI CCNNGG 4 cut(s) 106, 710, 759, 820
BseGI GGATG 5 cut(s) 46, 610, 891, 982, 994
BseLI CCNNNNNNNGG 3 cut(s) 234, 642, 1375
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 2 cut(s) 1041, 1266
BseNI ACTGG 4 cut(s) 33, 178, 1024, 1351
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 2 cut(s) 1203, 1367
BseX3I CGGCCG 1 cut(s) 1377
BseXI GCAGC 5 cut(s) 398, 533, 1298, 1372, 1411
BseYI CCCAGC 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 1380
BshFI GGCC 5 cut(s) 184, 960, 1001, 1379, 1505
BshNI GGYRCC 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 1380
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 2 cut(s) 573, 1229
BslFI GGGAC 2 cut(s) 807, 1363
BslI CCNNNNNNNGG 3 cut(s) 234, 642, 1375
BsmAI GTCTC 4 cut(s) 342, 345, 846, 1337
BsmBI CGTCTC 1 cut(s) 846
BsmFI GGGAC 2 cut(s) 807, 1363
BsnI GGCC 5 cut(s) 184, 960, 1001, 1379, 1505
Bsp1286I GDGCHC 3 cut(s) 295, 1203, 1367
Bsp1407I TGTACA 2 cut(s) 474, 1122
Bsp143I GATC 2 cut(s) 314, 1294
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 4 cut(s) 663, 745, 1452, 1477
BspANI GGCC 5 cut(s) 184, 960, 1001, 1379, 1505
BspCNI CTCAG 2 cut(s) 1042, 1267
BspHI TCATGA 1 cut(s) 1443
BspLI GGNNCC 9 cut(s) 316, 367, 453, 578, 961, 1000, 1142, 1191, 1221
BspPI GGATC 2 cut(s) 309, 322
BspQI GCTCTTC 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 451
BspTI CTTAAG 1 cut(s) 1247
BsrDI GCAATG 1 cut(s) 262
BsrGI TGTACA 2 cut(s) 474, 1122
BsrI ACTGG 4 cut(s) 33, 178, 1024, 1351
BssECI CCNNGG 4 cut(s) 106, 710, 759, 820
BssMI GATC 2 cut(s) 314, 1294
BssT1I CCWWGG 3 cut(s) 106, 710, 759
Bst2UI CCWGG 2 cut(s) 821, 873
Bst4CI ACNGT 4 cut(s) 124, 297, 548, 694
Bst6I CTCTTC 2 cut(s) 68, 1164
BstAFI CTTAAG 1 cut(s) 1247
BstAPI GCANNNNNTGC 1 cut(s) 467
BstAUI TGTACA 2 cut(s) 474, 1122
BstC8I GCNNGC 5 cut(s) 412, 1363, 1477, 1481, 1503
BstDEI CTNAG 3 cut(s) 913, 1050, 1275
BstDSI CCRYGG 1 cut(s) 710
BstEII GGTNACC 1 cut(s) 1416
BstF5I GGATG 5 cut(s) 46, 610, 891, 982, 994
BstKTI GATC 2 cut(s) 317, 1297
BstMAI GTCTC 4 cut(s) 342, 345, 846, 1337
BstMBI GATC 2 cut(s) 314, 1294
BstMCI CGRYCG 1 cut(s) 1380
BstMWI GCNNNNNNNGC 7 cut(s) 190, 383, 467, 1007, 1385, 1489, 1498
BstNI CCWGG 2 cut(s) 821, 873
BstNSI RCATGY 2 cut(s) 486, 1123
BstPI GGTNACC 1 cut(s) 1416
BstSCI CCNGG 3 cut(s) 572, 819, 871
BstSFI CTRYAG 1 cut(s) 1266
BstSLI GKGCMC 2 cut(s) 1203, 1367
BstV1I GCAGC 5 cut(s) 398, 533, 1298, 1372, 1411
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 1 cut(s) 311
BstYI RGATCY 1 cut(s) 314
BstZI CGGCCG 1 cut(s) 1377
BsuRI GGCC 5 cut(s) 184, 960, 1001, 1379, 1505
BtgI CCRYGG 1 cut(s) 710
BtrI CACGTC 1 cut(s) 1090
BtsCI GGATG 5 cut(s) 46, 610, 891, 982, 994
BtsI GCAGTG 2 cut(s) 372, 477
BtsIMutI CAGTG 3 cut(s) 171, 372, 477
Cac8I GCNNGC 5 cut(s) 412, 1363, 1477, 1481, 1503
CaiI CAGNNNCTG 2 cut(s) 389, 1274
CciI TCATGA 1 cut(s) 1443
Cfr13I GGNCC 5 cut(s) 218, 677, 959, 999, 1220
CseI GACGC 3 cut(s) 67, 515, 863
Csp6I GTAC 2 cut(s) 475, 1123
CviAII CATG 9 cut(s) 96, 362, 483, 590, 711, 1120, 1370, 1444, 1462
CviQI GTAC 2 cut(s) 475, 1123
DdeI CTNAG 3 cut(s) 913, 1050, 1275
DpnI GATC 2 cut(s) 316, 1296
DpnII GATC 2 cut(s) 314, 1294
EaeI YGGCCR 2 cut(s) 182, 1377
EagI CGGCCG 1 cut(s) 1377
Eam1104I CTCTTC 2 cut(s) 68, 1164
EarI CTCTTC 2 cut(s) 68, 1164
EclXI CGGCCG 1 cut(s) 1377
Eco130I CCWWGG 3 cut(s) 106, 710, 759
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 3 cut(s) 218, 677, 1220
Eco52I CGGCCG 1 cut(s) 1377
Eco91I GGTNACC 1 cut(s) 1416
EcoO109I RGGNCCY 2 cut(s) 959, 999
EcoO65I GGTNACC 1 cut(s) 1416
EcoRII CCWGG 2 cut(s) 819, 871
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 3 cut(s) 106, 710, 759
ErhI CCWWGG 3 cut(s) 106, 710, 759
Esp3I CGTCTC 1 cut(s) 846
FaeI CATG 9 cut(s) 99, 365, 486, 593, 714, 1123, 1373, 1447, 1465
FaqI GGGAC 2 cut(s) 807, 1363
FatI CATG 9 cut(s) 95, 361, 482, 589, 710, 1119, 1369, 1443, 1461
FauI CCCGC 1 cut(s) 1484
FauNDI CATATG 5 cut(s) 245, 811, 1195, 1291, 1494
FbaI TGATCA 1 cut(s) 1294
Fnu4HI GCNGC 5 cut(s) 387, 522, 1287, 1386, 1425
FokI GGATG 5 cut(s) 53, 617, 898, 989, 1001
Fsp4HI GCNGC 5 cut(s) 387, 522, 1287, 1386, 1425
FspBI CTAG 3 cut(s) 518, 1283, 1472
GluI GCNGC 5 cut(s) 387, 522, 1287, 1386, 1425
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 5 cut(s) 184, 960, 1001, 1379, 1505
HapII CCGG 2 cut(s) 573, 1229
HgaI GACGC 3 cut(s) 67, 515, 863
Hin1II CATG 9 cut(s) 99, 365, 486, 593, 714, 1123, 1373, 1447, 1465
HincII GTYRAC 3 cut(s) 553, 736, 1063
HindII GTYRAC 3 cut(s) 553, 736, 1063
HinfI GANTC 7 cut(s) 188, 213, 338, 350, 583, 1234, 1279
HpaII CCGG 2 cut(s) 573, 1229
HphI GGTGA 5 cut(s) 748, 1243, 1319, 1397, 1410
Hpy166II GTNNAC 4 cut(s) 475, 553, 736, 1063
Hpy188I TCNGA 4 cut(s) 290, 544, 681, 1094
Hpy188III TCNNGA 5 cut(s) 116, 312, 400, 891, 1444
Hpy8I GTNNAC 4 cut(s) 475, 553, 736, 1063
Hpy99I CGWCG 1 cut(s) 1094
HpyAV CCTTC 2 cut(s) 320, 668
HpyCH4III ACNGT 4 cut(s) 124, 297, 548, 694
HpyCH4IV ACGT 3 cut(s) 513, 1089, 1392
HpyF10VI GCNNNNNNNGC 7 cut(s) 190, 383, 467, 1007, 1385, 1489, 1498
HpyF3I CTNAG 3 cut(s) 913, 1050, 1275
HpySE526I ACGT 3 cut(s) 513, 1089, 1392
Hsp92II CATG 9 cut(s) 99, 365, 486, 593, 714, 1123, 1373, 1447, 1465
Ksp22I TGATCA 1 cut(s) 1294
Kzo9I GATC 2 cut(s) 314, 1294
LguI GCTCTTC 1 cut(s) 68
LmnI GCTCC 3 cut(s) 290, 1195, 1498
Lsp1109I GCAGC 5 cut(s) 398, 533, 1298, 1372, 1411
LweI GCATC 4 cut(s) 386, 523, 1091, 1303
MaeI CTAG 3 cut(s) 518, 1283, 1472
MaeII ACGT 3 cut(s) 513, 1089, 1392
MaeIII GTNAC 3 cut(s) 272, 668, 1416
MalI GATC 2 cut(s) 316, 1296
MboI GATC 2 cut(s) 314, 1294
MboII GAAGA 8 cut(s) 49, 85, 335, 503, 929, 932, 1112, 1181
MfeI CAATTG 3 cut(s) 615, 767, 1302
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 3 cut(s) 295, 1203, 1367
MlsI TGGCCA 1 cut(s) 184
MluNI TGGCCA 1 cut(s) 184
MlyI GAGTC 3 cut(s) 197, 344, 347
MmeI TCCRAC 2 cut(s) 190, 1072
Mox20I TGGCCA 1 cut(s) 184
MscI TGGCCA 1 cut(s) 184
MseI TTAA 5 cut(s) 126, 839, 1041, 1176, 1248
Msp20I TGGCCA 1 cut(s) 184
MspA1I CMGCKG 3 cut(s) 389, 665, 1424
MspCI CTTAAG 1 cut(s) 1247
MspI CCGG 2 cut(s) 573, 1229
MspR9I CCNGG 3 cut(s) 574, 821, 873
MunI CAATTG 3 cut(s) 615, 767, 1302
MvaI CCWGG 2 cut(s) 821, 873
MwoI GCNNNNNNNGC 7 cut(s) 190, 383, 467, 1007, 1385, 1489, 1498
NciI CCSGG 1 cut(s) 574
NcoI CCATGG 1 cut(s) 710
NdeI CATATG 5 cut(s) 245, 811, 1195, 1291, 1494
NdeII GATC 2 cut(s) 314, 1294
NlaIII CATG 9 cut(s) 99, 365, 486, 593, 714, 1123, 1373, 1447, 1465
NlaIV GGNNCC 9 cut(s) 316, 367, 453, 578, 961, 1000, 1142, 1191, 1221
NmuCI GTSAC 1 cut(s) 1416
NspI RCATGY 2 cut(s) 486, 1123
PagI TCATGA 1 cut(s) 1443
PciI ACATGT 1 cut(s) 1119
PciSI GCTCTTC 1 cut(s) 68
PfeI GAWTC 4 cut(s) 213, 583, 1234, 1279
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 5 cut(s) 388, 523, 1288, 1387, 1426
PleI GAGTC 3 cut(s) 196, 344, 346
PpsI GAGTC 3 cut(s) 196, 344, 346
PscI ACATGT 1 cut(s) 1119
PshBI ATTAAT 1 cut(s) 1176
Psp6I CCWGG 2 cut(s) 819, 871
PspEI GGTNACC 1 cut(s) 1416
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 2 cut(s) 819, 871
PspN4I GGNNCC 9 cut(s) 316, 367, 453, 578, 961, 1000, 1142, 1191, 1221
PspPI GGNCC 5 cut(s) 218, 677, 959, 999, 1220
PstNI CAGNNNCTG 2 cut(s) 389, 1274
PsuI RGATCY 1 cut(s) 314
PvuII CAGCTG 2 cut(s) 389, 1424
RsaI GTAC 2 cut(s) 476, 1124
RsaNI GTAC 2 cut(s) 475, 1123
SapI GCTCTTC 1 cut(s) 68
SaqAI TTAA 5 cut(s) 126, 839, 1041, 1176, 1248
SatI GCNGC 5 cut(s) 387, 522, 1287, 1386, 1425
Sau3AI GATC 2 cut(s) 314, 1294
Sau96I GGNCC 5 cut(s) 218, 677, 959, 999, 1220
SchI GAGTC 3 cut(s) 197, 344, 347
ScrFI CCNGG 3 cut(s) 574, 821, 873
SduI GDGCHC 3 cut(s) 295, 1203, 1367
SfaNI GCATC 4 cut(s) 386, 523, 1091, 1303
SfcI CTRYAG 1 cut(s) 1266
SinI GGWCC 3 cut(s) 218, 677, 1220
SmlI CTYRAG 1 cut(s) 1247
SmoI CTYRAG 1 cut(s) 1247
SsiI CCGC 4 cut(s) 663, 745, 1452, 1477
SspI AATATT 1 cut(s) 1114
SspMI CTAG 3 cut(s) 518, 1283, 1472
StyD4I CCNGG 3 cut(s) 572, 819, 871
StyI CCWWGG 3 cut(s) 106, 710, 759
TaaI ACNGT 4 cut(s) 124, 297, 548, 694
TaiI ACGT 3 cut(s) 516, 1092, 1395
TaqI TCGA 1 cut(s) 656
TaqII GACCGA 1 cut(s) 206
TatI WGTACW 2 cut(s) 474, 1122
TfiI GAWTC 4 cut(s) 213, 583, 1234, 1279
Tru1I TTAA 5 cut(s) 126, 839, 1041, 1176, 1248
Tru9I TTAA 5 cut(s) 126, 839, 1041, 1176, 1248
TscAI CASTG 3 cut(s) 178, 379, 477
TseFI GTSAC 1 cut(s) 1416
TseI GCWGC 5 cut(s) 386, 521, 1286, 1385, 1424
Tsp45I GTSAC 1 cut(s) 1416
TspDTI ATGAA 1 cut(s) 742
TspRI CASTG 3 cut(s) 178, 379, 477
Van91I CCANNNNNTGG 1 cut(s) 234
Vha464I CTTAAG 1 cut(s) 1247
VpaK11BI GGWCC 3 cut(s) 218, 677, 1220
VspI ATTAAT 1 cut(s) 1176
XapI RAATTY 4 cut(s) 177, 332, 827, 1486
XceI RCATGY 2 cut(s) 486, 1123
XcmI CCANNNNNNNNNTGG 2 cut(s) 231, 766
XspI CTAG 3 cut(s) 518, 1283, 1472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.