MD01G1171100.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003864
Physical Location & Seq
Reverse (-)
27397301 .. 27401453
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1171100.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGGAAGGAGGCCTGCGAGGTGCTATAGGCAGATTAAGAACAAGCCATACCCGAAATCGCGTTTCTGTCGCGGTGTCCCTGATCCCAAGATCAGAATTTATGATGTTGGAATGAAGAAGAGGGGTGTTGATGAGTTCCCCTTCTGTGTGCATCTTGTGAGTTGGGAGAAGGAGAATGTCTCGAGTGAGGCGCTTGAGGCAGCTCGCATCGCTTGCAACAAGTACATGACAAAGTATGCTGGGAAAGATACTTTCCATTTGAGAGTGAGGGTGCATCCGTTCCATGTTCTGCGCATCAACAAGATGCTTTCATGCGCTGGGGCTGATAGGCTCCAGACTGGAATGAGAGGTGCGTTTGGCAAGCCACTGGGAACATGTGCTCGAGTTGCTATCGGGCAAGTCCTCTTGTCTGTTCGCTGCAGGGATAGCAACAGTCAACATGCACAGGAGGCCCTCCGCCGTGCAAAGTTCAAGTTTCCTGGTCGCCAAAAGATTATTGTCAGCAGGAAGTGGGGTTTCACTAAGTTTAGCCGTGCTGATTACGTGAATTACAAGAAGGAAGGCCGAATCCAGCCAGATGGCGTTAACGCTAAGCTTCTTAGATGCCATGGTCCTCTGGCCAACCGTCAACCCGGAAAAGCATTCCTCACTCAACCTGCCCCATCGACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

225

Amino Acids

25.45

Weight (kDa)

10.51

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 12 - 166 4.2e-36 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 664
AccII CGCG 2 cut(s) 61, 72
AciI CCGC 2 cut(s) 72, 457
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 1 cut(s) 96
AfaI GTAC 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 1 cut(s) 472
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 2 cut(s) 203, 595
AluI AGCT 2 cut(s) 203, 595
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 2 cut(s) 181, 381
AoxI GGCC 4 cut(s) 11, 450, 562, 618
ApeKI GCWGC 2 cut(s) 200, 417
ApoI RAATTY 1 cut(s) 96
AspLEI GCGC 3 cut(s) 193, 294, 317
AspS9I GGNCC 2 cut(s) 451, 611
AsuC2I CCSGG 1 cut(s) 633
AvaI CYCGRG 2 cut(s) 181, 381
AvaII GGWCC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 212, 404
BccI CCATC 2 cut(s) 572, 670
BceAI ACGGC 2 cut(s) 444, 516
BciT130I CCWGG 1 cut(s) 480
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 184
BfmI CTRYAG 2 cut(s) 25, 418
BfoI RGCGCY 1 cut(s) 194
BfuAI ACCTGC 1 cut(s) 664
BisI GCNGC 2 cut(s) 201, 418
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 2 cut(s) 202, 419
Bme1390I CCNGG 2 cut(s) 480, 633
Bme18I GGWCC 1 cut(s) 611
BmeT110I CYCGRG 2 cut(s) 181, 381
BmgT120I GGNCC 2 cut(s) 451, 611
BmiI GGNNCC 1 cut(s) 332
BmrFI CCNGG 2 cut(s) 480, 633
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 6 cut(s) 160, 216, 283, 294, 303, 593
BmuI ACTGGG 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 317
Bpu1102I GCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 633
BsaAI YACGTR 1 cut(s) 544
BsaJI CCNNGG 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 343, 372
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 1 cut(s) 274
BseNI ACTGG 2 cut(s) 343, 372
BseXI GCAGC 2 cut(s) 212, 404
BseYI CCCAGC 2 cut(s) 239, 317
Bsh1236I CGCG 2 cut(s) 61, 72
BshFI GGCC 4 cut(s) 13, 452, 564, 620
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 181, 381
BsiSI CCGG 1 cut(s) 633
BslFI GGGAC 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 4 cut(s) 13, 452, 564, 620
BsoBI CYCGRG 2 cut(s) 181, 381
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 2 cut(s) 82, 90
Bsp1720I GCTNAGC 1 cut(s) 591
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 2 cut(s) 72, 457
BspANI GGCC 4 cut(s) 13, 452, 564, 620
BspFNI CGCG 2 cut(s) 61, 72
BspLI GGNNCC 1 cut(s) 332
BspMAI CTGCAG 1 cut(s) 422
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 2 cut(s) 343, 372
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 2 cut(s) 82, 90
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 434, 626
Bst6I CTCTTC 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 544
BstC8I GCNNGC 4 cut(s) 15, 205, 214, 362
BstDEI CTNAG 3 cut(s) 522, 591, 599
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 1 cut(s) 274
BstFNI CGCG 2 cut(s) 61, 72
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 3 cut(s) 193, 294, 317
BstKTI GATC 2 cut(s) 85, 93
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 2 cut(s) 82, 90
BstMWI GCNNNNNNNGC 6 cut(s) 197, 209, 213, 386, 426, 449
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 2 cut(s) 378, 443
BstSCI CCNGG 2 cut(s) 478, 631
BstSFI CTRYAG 2 cut(s) 25, 418
BstUI CGCG 2 cut(s) 61, 72
BstV1I GCAGC 2 cut(s) 212, 404
BstXI CCANNNNNNTGG 1 cut(s) 578
BsuRI GGCC 4 cut(s) 13, 452, 564, 620
BtgI CCRYGG 1 cut(s) 607
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 365
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 4 cut(s) 15, 205, 214, 362
CfoI GCGC 3 cut(s) 193, 294, 317
Cfr13I GGNCC 2 cut(s) 451, 611
Csp6I GTAC 1 cut(s) 223
CviAII CATG 6 cut(s) 226, 284, 312, 375, 440, 608
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 3 cut(s) 522, 591, 599
DpnI GATC 2 cut(s) 84, 92
DpnII GATC 2 cut(s) 82, 90
EaeI YGGCCR 1 cut(s) 618
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 607
Eco147I AGGCCT 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 611
Eco88I CYCGRG 2 cut(s) 181, 381
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 6 cut(s) 229, 287, 315, 378, 443, 611
FaqI GGGAC 1 cut(s) 62
FatI CATG 6 cut(s) 225, 283, 311, 374, 439, 607
Fnu4HI GCNGC 2 cut(s) 201, 418
FokI GGATG 1 cut(s) 261
Fsp4HI GCNGC 2 cut(s) 201, 418
FspI TGCGCA 1 cut(s) 293
GlaI GCGC 3 cut(s) 192, 293, 316
GluI GCNGC 2 cut(s) 201, 418
GsaI CCCAGC 2 cut(s) 243, 321
GsuI CTGGAG 1 cut(s) 317
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 4 cut(s) 13, 452, 564, 620
HapII CCGG 1 cut(s) 633
HhaI GCGC 3 cut(s) 193, 294, 317
Hin1II CATG 6 cut(s) 229, 287, 315, 378, 443, 611
Hin6I GCGC 3 cut(s) 191, 292, 315
HinP1I GCGC 3 cut(s) 191, 292, 315
HincII GTYRAC 3 cut(s) 437, 586, 629
HindII GTYRAC 3 cut(s) 437, 586, 629
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 1 cut(s) 567
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 1 cut(s) 633
Hpy166II GTNNAC 3 cut(s) 437, 586, 629
Hpy188I TCNGA 1 cut(s) 95
Hpy188III TCNNGA 2 cut(s) 181, 334
Hpy8I GTNNAC 3 cut(s) 437, 586, 629
HpyAV CCTTC 4 cut(s) 151, 163, 550, 554
HpyCH4III ACNGT 2 cut(s) 434, 626
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 6 cut(s) 151, 216, 274, 420, 443, 464
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 209, 213, 386, 426, 449
HpyF3I CTNAG 3 cut(s) 522, 591, 599
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 6 cut(s) 229, 287, 315, 378, 443, 611
HspAI GCGC 3 cut(s) 191, 292, 315
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 2 cut(s) 82, 90
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 2 cut(s) 212, 404
LweI GCATC 6 cut(s) 160, 216, 283, 294, 303, 593
MaeII ACGT 1 cut(s) 543
MalI GATC 2 cut(s) 84, 92
MboI GATC 2 cut(s) 82, 90
MboII GAAGA 2 cut(s) 127, 130
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 620
MluCI AATT 2 cut(s) 96, 547
MluNI TGGCCA 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 88
Mox20I TGGCCA 1 cut(s) 620
MscI TGGCCA 1 cut(s) 620
MseI TTAA 2 cut(s) 36, 585
Msp20I TGGCCA 1 cut(s) 620
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 2 cut(s) 480, 633
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 2 cut(s) 61, 72
MwoI GCNNNNNNNGC 6 cut(s) 197, 209, 213, 386, 426, 449
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 607
NdeII GATC 2 cut(s) 82, 90
NlaIII CATG 6 cut(s) 229, 287, 315, 378, 443, 611
NlaIV GGNNCC 1 cut(s) 332
NsbI TGCGCA 1 cut(s) 293
NspI RCATGY 2 cut(s) 378, 443
PaeR7I CTCGAG 2 cut(s) 181, 381
PceI AGGCCT 1 cut(s) 13
PciI ACATGT 1 cut(s) 374
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 567
PkrI GCNGC 2 cut(s) 202, 419
Ppu21I YACGTR 1 cut(s) 544
PscI ACATGT 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 478
PspFI CCCAGC 2 cut(s) 239, 317
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 332
PspPI GGNCC 2 cut(s) 451, 611
PspXI VCTCGAGB 1 cut(s) 381
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PstI CTGCAG 1 cut(s) 422
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SaqAI TTAA 2 cut(s) 36, 585
SatI GCNGC 2 cut(s) 201, 418
Sau3AI GATC 2 cut(s) 82, 90
Sau96I GGNCC 2 cut(s) 451, 611
ScrFI CCNGG 2 cut(s) 480, 633
SduI GDGCHC 1 cut(s) 382
SetI ASST 6 cut(s) 23, 205, 352, 546, 597, 658
SfaNI GCATC 6 cut(s) 160, 216, 283, 294, 303, 593
SfcI CTRYAG 2 cut(s) 25, 418
Sfr274I CTCGAG 2 cut(s) 181, 381
SinI GGWCC 1 cut(s) 611
SlaI CTCGAG 2 cut(s) 181, 381
SmlI CTYRAG 3 cut(s) 181, 194, 381
SmoI CTYRAG 3 cut(s) 181, 194, 381
Sse9I AATT 2 cut(s) 96, 547
SseBI AGGCCT 1 cut(s) 13
SsiI CCGC 2 cut(s) 72, 457
StuI AGGCCT 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 478, 631
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 434, 626
TaiI ACGT 1 cut(s) 546
TaqI TCGA 3 cut(s) 182, 382, 665
TasI AATT 2 cut(s) 96, 547
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 2 cut(s) 36, 585
Tru9I TTAA 2 cut(s) 36, 585
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 200, 417
TspDTI ATGAA 2 cut(s) 128, 300
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 372
VpaK11BI GGWCC 1 cut(s) 611
XapI RAATTY 1 cut(s) 96
XceI RCATGY 2 cut(s) 378, 443
XhoI CTCGAG 2 cut(s) 181, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.