MD11G1051200.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003864
Physical Location & Seq
Reverse (-)
4381828 .. 4384502
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1051200.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGGGAGAAGACCCGCCAGATGCTACCGCCAGATTAAGAACAAACCCTACCCGAAATCCCGCTTCTGCCGCGGTGTGCCCGACCCCAAGATCCGAATCTATGATGTCGGCATGAAGAAGAAGGGCGTCGACGAGTTCCCCTTCTGCATTCACCTCGTGTCCTGGGAGAAGGAAAACGTTTCCAGCGAGGCTCTTGAGGCGGCGCGAATCGCCTGCAACAAGTACATGACCAAGTTCGCCGGCAAGGACACGTTCCACCTGAGGGTTAGGGTTCACCCCTTCCATGTCCTCCGAATTAACAAGATGCTTTCGTGCGCCGGAGCCGATAGGCTTCAGACTGGTATGCGCGGAGCTTTCGGGAAGCCGCTCGGGACCTGCGCTAGGGTTGCTATTGGGCAGGTGCTGCTCTCTGTTCGTTGCAAGGAAAGCAACAAGAGCCACGCAGCCGAGGCCCTCCGCCGTGCCAAGTTCAAGTTTCCCGGCCGCCAAAAGATCATTGAGAGCAGGAAATGGGGATTCACCAAGTTCAGCAGAGCTGACTATGTGAGGTTGAAGTCTGAGGGACGAATTGTACCTGATGGTGTCAATGCCAAGCTTTTGGGATGCCATGGATCTTTGGCGTTGCGTCGTCCTGGTGGAGCATTCATTGATGCTGCTGTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

222

Amino Acids

24.86

Weight (kDa)

10.43

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 12 - 166 1e-35 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 383, 388
AccBSI CCGCTC 1 cut(s) 367
AccI GTMKAC 1 cut(s) 129
AccII CGCG 3 cut(s) 72, 205, 348
AclI AACGTT 1 cut(s) 177
AclWI GGATC 2 cut(s) 85, 619
AcoI YGGCCR 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 1 cut(s) 126
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 2 cut(s) 224, 573
AfiI CCNNNNNNNGG 2 cut(s) 262, 381
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 2 cut(s) 472, 553
AjnI CCWGG 2 cut(s) 161, 631
AluBI AGCT 3 cut(s) 353, 536, 595
AluI AGCT 3 cut(s) 353, 536, 595
AlwI GGATC 2 cut(s) 85, 619
AlwNI CAGNNNCTG 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 2 cut(s) 450, 481
ApeKI GCWGC 3 cut(s) 403, 443, 653
AspLEI GCGC 4 cut(s) 205, 317, 348, 380
AspS9I GGNCC 2 cut(s) 372, 451
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 3 cut(s) 143, 266, 511
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 372
AxyI CCTNAGG 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 81
BauI CACGAG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 390, 455, 640
BccI CCATC 1 cut(s) 572
BceAI ACGGC 1 cut(s) 444
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 195, 229
BciT130I CCWGG 2 cut(s) 163, 633
BcnI CCSGG 1 cut(s) 480
BfaI CTAG 1 cut(s) 381
BfuAI ACCTGC 2 cut(s) 383, 388
BisI GCNGC 7 cut(s) 70, 201, 365, 404, 444, 484, 654
BlsI GCNGC 7 cut(s) 71, 202, 366, 405, 445, 485, 655
Bme1390I CCNGG 3 cut(s) 163, 480, 633
Bme18I GGWCC 1 cut(s) 372
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 372, 451
BmiI GGNNCC 2 cut(s) 322, 373
BmrFI CCNGG 3 cut(s) 163, 480, 633
BmsI GCATC 4 cut(s) 11, 294, 593, 640
BpiI GAAGAC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 480
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 4 cut(s) 70, 162, 447, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 381
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 343
Bse21I CCTNAGG 1 cut(s) 260
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 2 cut(s) 163, 633
BseDI CCNNGG 4 cut(s) 70, 162, 447, 607
BseGI GGATG 1 cut(s) 608
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 262, 381
BseMII CTCAG 2 cut(s) 251, 549
BseNI ACTGG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 81
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 3 cut(s) 390, 455, 640
Bsh1236I CGCG 3 cut(s) 72, 205, 348
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 2 cut(s) 452, 483
BsiEI CGRYCG 1 cut(s) 484
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 240, 318, 480
BslFI GGGAC 2 cut(s) 385, 576
BslI CCNNNNNNNGG 2 cut(s) 262, 381
BsmFI GGGAC 2 cut(s) 385, 576
BsmI GAATGC 2 cut(s) 147, 641
BsnI GGCC 2 cut(s) 452, 483
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 3 cut(s) 90, 492, 611
Bsp19I CCATGG 1 cut(s) 607
BspANI GGCC 2 cut(s) 452, 483
BspCNI CTCAG 2 cut(s) 252, 550
BspFNI CGCG 3 cut(s) 72, 205, 348
BspLI GGNNCC 2 cut(s) 322, 373
BspMI ACCTGC 2 cut(s) 383, 388
BspPI GGATC 2 cut(s) 85, 619
BsrBI CCGCTC 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 4 cut(s) 70, 162, 447, 607
BssMI GATC 3 cut(s) 90, 492, 611
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 607
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 163, 633
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 214, 241
BstDEI CTNAG 2 cut(s) 260, 558
BstDSI CCRYGG 2 cut(s) 70, 607
BstENI CCTNNNNNAGG 1 cut(s) 379
BstF5I GGATG 1 cut(s) 608
BstFNI CGCG 3 cut(s) 72, 205, 348
BstHHI GCGC 4 cut(s) 205, 317, 348, 380
BstKTI GATC 3 cut(s) 93, 495, 614
BstMBI GATC 3 cut(s) 90, 492, 611
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 8 cut(s) 69, 197, 209, 386, 403, 426, 435, 449
BstNI CCWGG 2 cut(s) 163, 633
BstSCI CCNGG 3 cut(s) 161, 478, 631
BstSLI GKGCMC 1 cut(s) 81
BstUI CGCG 3 cut(s) 72, 205, 348
BstV1I GCAGC 3 cut(s) 390, 455, 640
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 2 cut(s) 90, 611
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 2 cut(s) 90, 611
BstZI CGGCCG 1 cut(s) 481
Bsu36I CCTNAGG 1 cut(s) 260
BsuRI GGCC 2 cut(s) 452, 483
BtgI CCRYGG 2 cut(s) 70, 607
BtsCI GGATG 1 cut(s) 608
BveI ACCTGC 2 cut(s) 383, 388
Cac8I GCNNGC 2 cut(s) 214, 241
CaiI CAGNNNCTG 1 cut(s) 403
CfoI GCGC 4 cut(s) 205, 317, 348, 380
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 372, 451
Cfr42I CCGCGG 1 cut(s) 73
CseI GACGC 2 cut(s) 115, 614
Csp6I GTAC 2 cut(s) 223, 572
CviAII CATG 4 cut(s) 112, 226, 284, 608
CviQI GTAC 2 cut(s) 223, 572
DdeI CTNAG 2 cut(s) 260, 558
DpnI GATC 3 cut(s) 92, 494, 613
DpnII GATC 3 cut(s) 90, 492, 611
DraIII CACNNNGTG 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 481
EagI CGGCCG 1 cut(s) 481
EciI GGCGGA 1 cut(s) 446
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 372
Eco52I CGGCCG 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 317
Eco81I CCTNAGG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 2 cut(s) 372, 451
EcoRII CCWGG 2 cut(s) 161, 631
EcoT14I CCWWGG 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 4 cut(s) 115, 229, 287, 611
FaiI YATR 7 cut(s) 102, 113, 227, 285, 344, 543, 609
FaqI GGGAC 2 cut(s) 385, 576
FatI CATG 4 cut(s) 111, 225, 283, 607
FauI CCCGC 2 cut(s) 22, 68
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 7 cut(s) 70, 201, 365, 404, 444, 484, 654
FokI GGATG 1 cut(s) 615
Fsp4HI GCNGC 7 cut(s) 70, 201, 365, 404, 444, 484, 654
FspBI CTAG 1 cut(s) 381
GlaI GCGC 4 cut(s) 204, 316, 347, 379
GluI GCNGC 7 cut(s) 70, 201, 365, 404, 444, 484, 654
HaeIII GGCC 2 cut(s) 452, 483
HapII CCGG 3 cut(s) 240, 318, 480
HgaI GACGC 2 cut(s) 115, 614
HhaI GCGC 4 cut(s) 205, 317, 348, 380
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 115, 229, 287, 611
Hin6I GCGC 4 cut(s) 203, 315, 346, 378
HinP1I GCGC 4 cut(s) 203, 315, 346, 378
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 3 cut(s) 96, 207, 516
HpaII CCGG 3 cut(s) 240, 318, 480
HphI GGTGA 3 cut(s) 143, 266, 511
Hpy166II GTNNAC 3 cut(s) 130, 274, 661
Hpy188I TCNGA 4 cut(s) 95, 293, 336, 559
Hpy188III TCNNGA 3 cut(s) 194, 358, 370
Hpy8I GTNNAC 3 cut(s) 130, 274, 661
Hpy99I CGWCG 3 cut(s) 131, 134, 630
HpyAV CCTTC 4 cut(s) 115, 151, 163, 289
HpyCH4IV ACGT 2 cut(s) 177, 251
HpyCH4V TGCA 3 cut(s) 147, 216, 420
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 197, 209, 386, 403, 426, 435, 449
HpyF3I CTNAG 2 cut(s) 260, 558
HpySE526I ACGT 2 cut(s) 177, 251
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 115, 229, 287, 611
HspAI GCGC 4 cut(s) 203, 315, 346, 378
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 3 cut(s) 90, 492, 611
LmnI GCTCC 3 cut(s) 320, 350, 638
Lsp1109I GCAGC 3 cut(s) 390, 455, 640
LweI GCATC 4 cut(s) 11, 294, 593, 640
MaeI CTAG 1 cut(s) 381
MaeII ACGT 2 cut(s) 177, 251
MalI GATC 3 cut(s) 92, 494, 613
MbiI CCGCTC 1 cut(s) 367
MboI GATC 3 cut(s) 90, 492, 611
MboII GAAGA 3 cut(s) 21, 127, 130
MflI RGATCY 2 cut(s) 90, 611
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 2 cut(s) 294, 567
MnlI CCTC 9 cut(s) 164, 181, 190, 255, 299, 442, 464, 540, 553
MroNI GCCGGC 1 cut(s) 239
MseI TTAA 2 cut(s) 36, 297
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 3 cut(s) 240, 318, 480
MspR9I CCNGG 3 cut(s) 163, 480, 633
Mva1269I GAATGC 2 cut(s) 147, 641
MvaI CCWGG 2 cut(s) 163, 633
MvnI CGCG 3 cut(s) 72, 205, 348
MwoI GCNNNNNNNGC 8 cut(s) 69, 197, 209, 386, 403, 426, 435, 449
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 480
NcoI CCATGG 1 cut(s) 607
NdeII GATC 3 cut(s) 90, 492, 611
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 4 cut(s) 115, 229, 287, 611
NlaIV GGNNCC 2 cut(s) 322, 373
NmeAIII GCCGAG 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 2 cut(s) 147, 641
PdiI GCCGGC 1 cut(s) 241
PfeI GAWTC 3 cut(s) 96, 207, 516
PkrI GCNGC 7 cut(s) 71, 202, 366, 405, 445, 485, 655
PpuMI RGGWCCY 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 161, 631
PspGI CCWGG 2 cut(s) 161, 631
PspN4I GGNNCC 2 cut(s) 322, 373
PspPI GGNCC 2 cut(s) 372, 451
PspPPI RGGWCCY 1 cut(s) 372
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 90, 611
RsaI GTAC 2 cut(s) 224, 573
RsaNI GTAC 2 cut(s) 223, 572
SacII CCGCGG 1 cut(s) 73
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 36, 297
SatI GCNGC 7 cut(s) 70, 201, 365, 404, 444, 484, 654
Sau3AI GATC 3 cut(s) 90, 492, 611
Sau96I GGNCC 2 cut(s) 372, 451
ScrFI CCNGG 3 cut(s) 163, 480, 633
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 4 cut(s) 11, 294, 593, 640
Sfr303I CCGCGG 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 73
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 2 cut(s) 294, 567
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 161, 478, 631
StyI CCWWGG 1 cut(s) 607
TaiI ACGT 2 cut(s) 180, 254
TaqI TCGA 1 cut(s) 129
TasI AATT 2 cut(s) 294, 567
TatI WGTACW 1 cut(s) 222
TauI GCSGC 4 cut(s) 72, 203, 367, 486
TfiI GAWTC 3 cut(s) 96, 207, 516
Tru1I TTAA 2 cut(s) 36, 297
Tru9I TTAA 2 cut(s) 36, 297
TseI GCWGC 3 cut(s) 403, 443, 653
TspDTI ATGAA 2 cut(s) 128, 634
VpaK11BI GGWCC 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.