FvH4_7g26240

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003864
Physical Location & Seq
Reverse (-)
19885511 .. 19887400
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26240.t1

Sequence Viewer

Length: 666 bp
ATGGGGAGGAGACCTGCGAGGTGCTACAGACAAATAAAAAACAAGCCGTACCCCAAGTCACGGTTCTGTCGTGGTGTCCCTGATCCTAAGATCAGAATCTATGATGTTGGCATGAAGAAGAAGGGTGTTGATGAGTTCCCCTTCTGTGTTCACCTTGTCAGTTGGGAGAAGGAAAATGTGTCGAGTGAGGCTCTTGAGGCTGCTCGTATTGCATGCAACAAATACATGGCCAAGTTTGCTGGGAAGGATGCTTTCCACTTGAGAGTCAGGGTGCATCCGTTCCATGTTCTGCGCATCAACAAGATGCTTTCATGTGCTGGGGCTGATAGGCTCCAGACTGGAATGAGGGGTGCTTTTGGAAAGCCGCTTGGAACTTGTGCTCGGGTTGCTATTGGACAGGTCCTTCTTTCTGTTCGCTGCAAGGATAGCAACAGTCACCATGCACAGGAGGCCCTCCGTCGTGCCAAGTTCAAGTTTCCTGGTCGCCAAAAGATCATTGTTAGCAGGAAGTGGGGATTCACCAAGTTTAACCGTAATGACTATGTGAAGTTCAAGTCTGAGGGCAAAATTCAACCAGATGGTGTTAATGCTAAGCTTTATGGGTGCCATGGTGCTCTTGCTAACCGTCAACCTGGAAAAGCTTTCCTGTCTGCACCCGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

222

Amino Acids

24.92

Weight (kDa)

10.36

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 12 - 166 1.3e-35 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 603
AciI CCGC 1 cut(s) 365
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 567
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 60, 445
AgsI TTSAA 3 cut(s) 472, 553, 572
AjnI CCWGG 2 cut(s) 478, 631
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AluBI AGCT 2 cut(s) 595, 641
AluI AGCT 2 cut(s) 595, 641
Alw21I GWGCWC 2 cut(s) 382, 616
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 2 cut(s) 228, 450
ApeKI GCWGC 2 cut(s) 200, 417
ApoI RAATTY 1 cut(s) 567
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 2 cut(s) 400, 451
AsuHPI GGTGA 3 cut(s) 143, 428, 511
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 1 cut(s) 400
BalI TGGCCA 1 cut(s) 230
BanI GGYRCC 1 cut(s) 603
Bbv12I GWGCWC 2 cut(s) 382, 616
BbvI GCAGC 2 cut(s) 187, 404
BccI CCATC 1 cut(s) 572
BceAI ACGGC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 480, 633
BcoDI GTCTC 1 cut(s) 4
BfmI CTRYAG 1 cut(s) 25
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 3 cut(s) 201, 365, 418
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 3 cut(s) 202, 366, 419
Bme1390I CCNGG 2 cut(s) 480, 633
Bme18I GGWCC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 2 cut(s) 400, 451
BmiI GGNNCC 2 cut(s) 332, 605
BmrFI CCNGG 2 cut(s) 480, 633
BmsI GCATC 4 cut(s) 238, 283, 294, 303
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 317
Bpu1102I GCTNAGC 1 cut(s) 591
BpuEI CTTGAG 2 cut(s) 215, 280
BsaBI GATNNNNATC 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 1 cut(s) 607
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 445
Bse1I ACTGG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 2 cut(s) 480, 633
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 2 cut(s) 253, 274
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 60, 445
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 187, 404
BseYI CCCAGC 2 cut(s) 239, 317
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 2 cut(s) 230, 452
BshNI GGYRCC 1 cut(s) 603
BsiHKAI GWGCWC 2 cut(s) 382, 616
BsiHKCI CYCGRG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 60, 445
BsmAI GTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 2 cut(s) 230, 452
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 382, 616
Bsp143I GATC 3 cut(s) 82, 90, 492
Bsp1720I GCTNAGC 1 cut(s) 591
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 1 cut(s) 365
BspANI GGCC 2 cut(s) 230, 452
BspCNI CTCAG 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 332, 605
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 603
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 3 cut(s) 82, 90, 492
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 2 cut(s) 480, 633
Bst4CI ACNGT 4 cut(s) 63, 434, 533, 626
BstAPI GCANNNNNTGC 1 cut(s) 659
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 3 cut(s) 87, 558, 591
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 2 cut(s) 253, 274
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 3 cut(s) 85, 93, 495
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 3 cut(s) 82, 90, 492
BstMWI GCNNNNNNNGC 7 cut(s) 197, 209, 236, 386, 426, 449, 659
BstNI CCWGG 2 cut(s) 480, 633
BstNSI RCATGY 1 cut(s) 216
BstSCI CCNGG 2 cut(s) 478, 631
BstSFI CTRYAG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 187, 404
BsuRI GGCC 2 cut(s) 230, 452
BtgI CCRYGG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 253, 274
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 214
CfoI GCGC 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 400, 451
Csp6I GTAC 1 cut(s) 49
CviAII CATG 7 cut(s) 112, 213, 226, 284, 312, 440, 608
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 87, 558, 591
DpnI GATC 3 cut(s) 84, 92, 494
DpnII GATC 3 cut(s) 82, 90, 492
EaeI YGGCCR 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 607
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 2 cut(s) 400, 451
EcoRII CCWGG 2 cut(s) 478, 631
EcoT14I CCWWGG 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 7 cut(s) 115, 216, 229, 287, 315, 443, 611
FaqI GGGAC 1 cut(s) 62
FatI CATG 7 cut(s) 111, 212, 225, 283, 311, 439, 607
Fnu4HI GCNGC 3 cut(s) 201, 365, 418
FokI GGATG 2 cut(s) 260, 261
Fsp4HI GCNGC 3 cut(s) 201, 365, 418
FspI TGCGCA 1 cut(s) 293
GlaI GCGC 1 cut(s) 293
GluI GCNGC 3 cut(s) 201, 365, 418
GsaI CCCAGC 2 cut(s) 243, 321
GsuI CTGGAG 1 cut(s) 317
HaeIII GGCC 2 cut(s) 230, 452
HhaI GCGC 1 cut(s) 294
Hin1II CATG 7 cut(s) 115, 216, 229, 287, 315, 443, 611
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 629
HindII GTYRAC 1 cut(s) 629
HindIII AAGCTT 2 cut(s) 593, 639
HinfI GANTC 3 cut(s) 96, 264, 516
HphI GGTGA 3 cut(s) 143, 428, 511
Hpy166II GTNNAC 2 cut(s) 151, 629
Hpy188I TCNGA 2 cut(s) 95, 559
Hpy188III TCNNGA 2 cut(s) 194, 334
Hpy8I GTNNAC 2 cut(s) 151, 629
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 5 cut(s) 115, 151, 163, 238, 413
HpyCH4III ACNGT 4 cut(s) 63, 434, 533, 626
HpyCH4V TGCA 6 cut(s) 212, 216, 274, 420, 443, 653
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 209, 236, 386, 426, 449, 659
HpyF3I CTNAG 3 cut(s) 87, 558, 591
Hsp92II CATG 7 cut(s) 115, 216, 229, 287, 315, 443, 611
HspAI GCGC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 82, 90, 492
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 2 cut(s) 187, 404
LweI GCATC 4 cut(s) 238, 283, 294, 303
MaeIII GTNAC 2 cut(s) 57, 434
MalI GATC 3 cut(s) 84, 92, 494
MboI GATC 3 cut(s) 82, 90, 492
MboII GAAGA 2 cut(s) 127, 130
MhlI GDGCHC 2 cut(s) 382, 616
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 1 cut(s) 567
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 7 cut(s) 12, 181, 190, 339, 442, 464, 553
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 528, 585
Msp20I TGGCCA 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 480, 633
MvaI CCWGG 2 cut(s) 480, 633
MwoI GCNNNNNNNGC 7 cut(s) 197, 209, 236, 386, 426, 449, 659
NcoI CCATGG 1 cut(s) 607
NdeII GATC 3 cut(s) 82, 90, 492
NlaIII CATG 7 cut(s) 115, 216, 229, 287, 315, 443, 611
NlaIV GGNNCC 2 cut(s) 332, 605
NmuCI GTSAC 2 cut(s) 57, 434
NsbI TGCGCA 1 cut(s) 293
NspI RCATGY 1 cut(s) 216
PaeI GCATGC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PfeI GAWTC 2 cut(s) 96, 516
PkrI GCNGC 3 cut(s) 202, 366, 419
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 2 cut(s) 478, 631
PspFI CCCAGC 2 cut(s) 239, 317
PspGI CCWGG 2 cut(s) 478, 631
PspN4I GGNNCC 2 cut(s) 332, 605
PspPI GGNCC 2 cut(s) 400, 451
PspPPI RGGWCCY 1 cut(s) 400
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 528, 585
SatI GCNGC 3 cut(s) 201, 365, 418
Sau3AI GATC 3 cut(s) 82, 90, 492
Sau96I GGNCC 2 cut(s) 400, 451
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 2 cut(s) 480, 633
SduI GDGCHC 2 cut(s) 382, 616
SetI ASST 7 cut(s) 16, 23, 156, 402, 597, 634, 643
SfaNI GCATC 4 cut(s) 238, 283, 294, 303
SfcI CTRYAG 1 cut(s) 25
SinI GGWCC 1 cut(s) 400
SmlI CTYRAG 2 cut(s) 194, 259
SmoI CTYRAG 2 cut(s) 194, 259
SphI GCATGC 1 cut(s) 216
Sse9I AATT 1 cut(s) 567
SsiI CCGC 1 cut(s) 365
StyD4I CCNGG 2 cut(s) 478, 631
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 4 cut(s) 63, 434, 533, 626
TaqI TCGA 1 cut(s) 182
TasI AATT 1 cut(s) 567
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 2 cut(s) 96, 516
Tru1I TTAA 2 cut(s) 528, 585
Tru9I TTAA 2 cut(s) 528, 585
TseFI GTSAC 2 cut(s) 57, 434
TseI GCWGC 2 cut(s) 200, 417
Tsp45I GTSAC 2 cut(s) 57, 434
TspDTI ATGAA 2 cut(s) 128, 300
TspGWI ACGGA 2 cut(s) 267, 446
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 1 cut(s) 567
XceI RCATGY 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.