Rorug05G0425900

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003864
Physical Location & Seq
Reverse (-)
59176817 .. 59181801
4985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0425900.1

Sequence Viewer

Length: 1227 bp
ATGCAAAGACTGGTTTCAGCGAGGTCTGGGATTACAAGTCTTCTGAGAAATCAGAGACGCGCGGCTTCTTTCACCACCGCTTTACTCTTCGATGATACTCAAATTCAGTTTAAGGAAAGTGTGGGTCAGTTTGCACAAGAACACATTGCTCCGCATGCTTCAAAAGTAGACCAAACTAATAATTTTCCCAAGGAGGTTAACTTATGGAAGCTCATGGGAGAGTTTAATCTTCATGGAATTACTGCCCCAGAGGAATATGGAGGGCTGGGTCTTGGCTACTTGTATCACTGCATTGCTATGGAAGAAATAAGCCGTGCTTCAGGATCTGTTGGACTATCATATGGTGCCCATTCCAACCTGTGCATTAATCAATTGGTGAGGCATGGAAATCCTACTCAGAAAGAGAAGTATTTGCCAAAGCTTATTAGTGGAGAGCATGTAGGGGCTCTTGCAATGAGCGAGCCCAATTCTGGTTCTGACGTAGTTAGCATGAAGTGCAAAGCTGATCGAGCAGATGGGGGTTATATCTTAAATGGGAACAAGATGTGGTGCACCAATGGACCAGTTGCTCAAACATTGGTTGTTTATGCAAAAACGGACATAAATGCGGGCTCAAAGGGAATCACAGCATTCATCATTGAGAAGGGGATGCCCGGATTCAGTACTGCCCAGAAATTAGACAAACTCGGCATGCGAGGAAGCGATACATGTGAGCTTGTCTTTGAGAATTGCTTTGTTCCGAATGAAAATGTTCTTGGCCAGGAAGGAAAAGGAGTCTATGTCATGATGTCAGGGTTAGATTTAGAGAGGCTTGTTTTAGCAGGTGGGCCCCTAGGTATTATGCAGGCATGTATGGATGTTGTCCTTCCTTATGTTCGACAGCGAGAGCAGTTTGGCCGTCCTATTGGGGAGTTTCAGCTTATACAGGGGAAAATTGCTGACATGTATACTTCTTTACAATCTTCAAGGGCCTATGTGTATTCTGTTGCAAGGGAATGTGACAATGGAAGAGTTGACCCAAAGGACTGTGCAGGAGTTATACTATCTACGGCTGAAAGAGCCACGCAGGTTGCTTTGCAGGCTATACAATGCTTAGGTGGTAATGGCTATGTAAATGAGTACCCAACCGGCCGTCTCTTTCGAGATGCCAAACTCTATGAGATTGGAGCAGGGACTAGTGAGATCAGAAGGATGATAATTGGTCGTGGGCTATTTAAGGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

408

Amino Acids

44.53

Weight (kDa)

7.53

Isoelectric Point (pI)

28.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-CoA_dh_N PF02771 32 - 145 7.2e-39 Acyl-CoA dehydrogenase, N-terminal domain
Acyl-CoA_dh_M PF02770 149 - 244 1.3e-25 Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1 PF00441 256 - 404 6.5e-45 Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2 PF08028 273 - 387 3.3e-11 Acyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 2 cut(s) 812, 1057
AccB1I GGYRCC 1 cut(s) 344
AccI GTMKAC 2 cut(s) 168, 947
AccII CGCG 2 cut(s) 60, 62
AciI CCGC 4 cut(s) 62, 78, 152, 608
AclWI GGATC 1 cut(s) 331
AcoI YGGCCR 3 cut(s) 757, 895, 1129
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 2 cut(s) 664, 1121
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 2 cut(s) 707, 942
AgsI TTSAA 2 cut(s) 162, 966
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 1 cut(s) 759
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AluBI AGCT 5 cut(s) 211, 421, 503, 715, 919
AluI AGCT 5 cut(s) 211, 421, 503, 715, 919
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 49, 1139
Alw44I GTGCAC 1 cut(s) 550
AlwI GGATC 1 cut(s) 331
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 5 cut(s) 757, 827, 895, 969, 1129
ApaI GGGCCC 1 cut(s) 831
ApaLI GTGCAC 1 cut(s) 550
ApoI RAATTY 1 cut(s) 102
AseI ATTAAT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 750
AspA2I CCTAGG 1 cut(s) 832
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 4 cut(s) 560, 827, 828, 969
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 2 cut(s) 64, 388
AvaII GGWCC 1 cut(s) 560
AvrII CCTAGG 1 cut(s) 832
BaeGI GKGCMC 3 cut(s) 349, 554, 831
BalI TGGCCA 1 cut(s) 759
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 4 cut(s) 448, 465, 614, 831
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 554
BccI CCATC 1 cut(s) 509
BceAI ACGGC 4 cut(s) 297, 882, 1065, 1116
BciT130I CCWGG 1 cut(s) 761
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 2 cut(s) 49, 1139
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 2 cut(s) 833, 1176
BfuAI ACCTGC 2 cut(s) 812, 1057
BisI GCNGC 1 cut(s) 63
BlnI CCTAGG 1 cut(s) 832
BlsI GCNGC 1 cut(s) 64
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 2 cut(s) 654, 761
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 4 cut(s) 560, 827, 828, 969
BmiI GGNNCC 3 cut(s) 346, 829, 830
BmrFI CCNGG 2 cut(s) 654, 761
BmsI GCATC 2 cut(s) 639, 1135
BpiI GAAGAC 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 1093
BpuMI CCSGG 1 cut(s) 654
BsaJI CCNNGG 2 cut(s) 189, 832
BsaXI ACNNNNNCTCC 4 cut(s) 423, 453, 765, 795
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 2 cut(s) 15, 563
Bse3DI GCAATG 3 cut(s) 144, 291, 459
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 2 cut(s) 189, 832
BseGI GGATG 3 cut(s) 654, 862, 1197
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 3 cut(s) 144, 291, 459
BseMII CTCAG 2 cut(s) 35, 410
BseNI ACTGG 2 cut(s) 15, 563
BseSI GKGCMC 3 cut(s) 349, 554, 831
BseX3I CGGCCG 1 cut(s) 1129
BseYI CCCAGC 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 1050
Bsh1236I CGCG 2 cut(s) 60, 62
Bsh1285I CGRYCG 1 cut(s) 1132
BshFI GGCC 5 cut(s) 759, 829, 897, 971, 1131
BshNI GGYRCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 1132
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 2 cut(s) 654, 1128
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 49, 1139
BsmBI CGTCTC 2 cut(s) 49, 1139
BsmFI GGGAC 1 cut(s) 1186
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 5 cut(s) 759, 829, 897, 971, 1131
Bsp120I GGGCCC 1 cut(s) 827
Bsp1286I GDGCHC 6 cut(s) 349, 448, 465, 554, 614, 831
Bsp143I GATC 3 cut(s) 323, 505, 1182
BspACI CCGC 4 cut(s) 62, 78, 152, 608
BspANI GGCC 5 cut(s) 759, 829, 897, 971, 1131
BspCNI CTCAG 2 cut(s) 36, 409
BspFNI CGCG 2 cut(s) 60, 62
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 3 cut(s) 346, 829, 830
BspMI ACCTGC 2 cut(s) 812, 1057
BspPI GGATC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 344
BsrDI GCAATG 3 cut(s) 144, 291, 459
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 2 cut(s) 15, 563
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 2 cut(s) 189, 832
BssMI GATC 3 cut(s) 323, 505, 1182
BssNAI GTATAC 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 189, 832
Bst1107I GTATAC 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 2 cut(s) 92, 1003
BstAPI GCANNNNNTGC 1 cut(s) 495
BstC8I GCNNGC 6 cut(s) 156, 461, 610, 692, 846, 1080
BstDEI CTNAG 3 cut(s) 44, 396, 1093
BstF5I GGATG 3 cut(s) 654, 862, 1197
BstFNI CGCG 2 cut(s) 60, 62
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 3 cut(s) 326, 508, 1185
BstMAI GTCTC 2 cut(s) 49, 1139
BstMBI GATC 3 cut(s) 323, 505, 1182
BstMCI CGRYCG 1 cut(s) 1132
BstMWI GCNNNNNNNGC 5 cut(s) 155, 495, 509, 1058, 1079
BstNI CCWGG 1 cut(s) 761
BstNSI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
BstSCI CCNGG 2 cut(s) 652, 759
BstSLI GKGCMC 3 cut(s) 349, 554, 831
BstUI CGCG 2 cut(s) 60, 62
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BstZ17I GTATAC 1 cut(s) 948
BstZI CGGCCG 1 cut(s) 1129
BsuRI GGCC 5 cut(s) 759, 829, 897, 971, 1131
BtsCI GGATG 3 cut(s) 654, 862, 1197
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 286
BveI ACCTGC 2 cut(s) 812, 1057
Cac8I GCNNGC 6 cut(s) 156, 461, 610, 692, 846, 1080
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 783
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 4 cut(s) 560, 827, 828, 969
CseI GACGC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 663, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 2 cut(s) 663, 1120
DdeI CTNAG 3 cut(s) 44, 396, 1093
DpnI GATC 3 cut(s) 325, 507, 1184
DpnII GATC 3 cut(s) 323, 505, 1182
EaeI YGGCCR 3 cut(s) 757, 895, 1129
EagI CGGCCG 1 cut(s) 1129
Eam1104I CTCTTC 2 cut(s) 92, 1003
EarI CTCTTC 2 cut(s) 92, 1003
EclXI CGGCCG 1 cut(s) 1129
Eco130I CCWWGG 2 cut(s) 189, 832
Eco24I GRGCYC 4 cut(s) 448, 465, 614, 831
Eco47I GGWCC 1 cut(s) 560
Eco52I CGGCCG 1 cut(s) 1129
Eco57I CTGAAG 1 cut(s) 303
EcoO109I RGGNCCY 2 cut(s) 828, 969
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 2 cut(s) 189, 832
EcoT38I GRGCYC 4 cut(s) 448, 465, 614, 831
ErhI CCWWGG 2 cut(s) 189, 832
Esp3I CGTCTC 2 cut(s) 49, 1139
FalI AAGNNNNNCTT 2 cut(s) 301, 333
FaqI GGGAC 1 cut(s) 1186
FauI CCCGC 1 cut(s) 601
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 2 cut(s) 168, 947
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 3 cut(s) 661, 869, 1204
FriOI GRGCYC 4 cut(s) 448, 465, 614, 831
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 833, 1176
GlaI GCGC 1 cut(s) 61
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 5 cut(s) 759, 829, 897, 971, 1131
HapII CCGG 2 cut(s) 654, 1128
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 62
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 2 cut(s) 199, 1015
HindII GTYRAC 2 cut(s) 199, 1015
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 3 cut(s) 621, 657, 774
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 2 cut(s) 654, 1128
HphI GGTGA 2 cut(s) 64, 388
Hpy166II GTNNAC 5 cut(s) 169, 199, 552, 948, 1015
Hpy188I TCNGA 6 cut(s) 45, 54, 399, 478, 741, 1187
Hpy188III TCNNGA 3 cut(s) 321, 784, 1142
Hpy8I GTNNAC 5 cut(s) 169, 199, 552, 948, 1015
HpyAV CCTTC 4 cut(s) 637, 758, 875, 1182
HpyCH4III ACNGT 1 cut(s) 1028
HpyCH4IV ACGT 1 cut(s) 480
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 495, 509, 1058, 1079
HpyF3I CTNAG 3 cut(s) 44, 396, 1093
HpySE526I ACGT 1 cut(s) 480
HspAI GCGC 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 323, 505, 1182
LmnI GCTCC 3 cut(s) 154, 1166, 1219
LweI GCATC 2 cut(s) 639, 1135
MaeI CTAG 2 cut(s) 833, 1176
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 998
MalI GATC 3 cut(s) 325, 507, 1184
MboI GATC 3 cut(s) 323, 505, 1182
MboII GAAGA 6 cut(s) 32, 79, 221, 314, 954, 1020
MfeI CAATTG 1 cut(s) 371
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 6 cut(s) 349, 448, 465, 554, 614, 831
MlsI TGGCCA 1 cut(s) 759
MluCI AATT 9 cut(s) 102, 181, 237, 371, 466, 674, 727, 933, 1197
MluNI TGGCCA 1 cut(s) 759
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 2 cut(s) 310, 378
MnlI CCTC 7 cut(s) 15, 187, 244, 254, 372, 689, 801
Mox20I TGGCCA 1 cut(s) 759
MroXI GAANNNNTTC 1 cut(s) 750
MscI TGGCCA 1 cut(s) 759
MseI TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 759
MspI CCGG 2 cut(s) 654, 1128
MspR9I CCNGG 2 cut(s) 654, 761
MunI CAATTG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 629
MvaI CCWGG 1 cut(s) 761
MvnI CGCG 2 cut(s) 60, 62
MwoI GCNNNNNNNGC 5 cut(s) 155, 495, 509, 1058, 1079
NciI CCSGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 340
NdeII GATC 3 cut(s) 323, 505, 1182
NlaIV GGNNCC 3 cut(s) 346, 829, 830
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 998
NspI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
PaeI GCATGC 2 cut(s) 158, 694
PagI TCATGA 1 cut(s) 783
PaqCI CACCTGC 1 cut(s) 812
PciI ACATGT 2 cut(s) 707, 942
PctI GAATGC 1 cut(s) 629
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 2 cut(s) 621, 657
PkrI GCNGC 1 cut(s) 64
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
PscI ACATGT 2 cut(s) 707, 942
PshBI ATTAAT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 759
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 3 cut(s) 346, 829, 830
PspOMI GGGCCC 1 cut(s) 827
PspPI GGNCC 4 cut(s) 560, 827, 828, 969
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 2 cut(s) 664, 1121
RsaNI GTAC 2 cut(s) 663, 1120
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 3 cut(s) 323, 505, 1182
Sau96I GGNCC 4 cut(s) 560, 827, 828, 969
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 2 cut(s) 654, 761
SduI GDGCHC 6 cut(s) 349, 448, 465, 554, 614, 831
SfaNI GCATC 2 cut(s) 639, 1135
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 296
SpeI ACTAGT 1 cut(s) 1175
SphI GCATGC 2 cut(s) 158, 694
Sse9I AATT 9 cut(s) 102, 181, 237, 371, 466, 674, 727, 933, 1197
SsiI CCGC 4 cut(s) 62, 78, 152, 608
SspMI CTAG 2 cut(s) 833, 1176
StyD4I CCNGG 2 cut(s) 652, 759
StyI CCWWGG 2 cut(s) 189, 832
TaaI ACNGT 1 cut(s) 1028
TaiI ACGT 1 cut(s) 483
TaqI TCGA 4 cut(s) 90, 508, 877, 1141
TasI AATT 9 cut(s) 102, 181, 237, 371, 466, 674, 727, 933, 1197
TatI WGTACW 1 cut(s) 662
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 621, 657
Tru1I TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
Tru9I TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 1 cut(s) 998
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 4 cut(s) 221, 506, 622, 759
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 1 cut(s) 293
VneI GTGCAC 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 560
VspI ATTAAT 1 cut(s) 366
XapI RAATTY 1 cut(s) 102
XceI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
XmaJI CCTAGG 1 cut(s) 832
XmiI GTMKAC 2 cut(s) 168, 947
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 2 cut(s) 833, 1176
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.