MD03G1048400.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003864
Physical Location & Seq
Reverse (-)
3878182 .. 3879871
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1048400.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGGGAGAAGACCCGCTAGATGCTACCGCCAGATCAAGAACAAGCCCTACCCGAAATCCCGCTTCTGCCGCGGTGTGCCAGACCCGAAGATCCGAATCTATGACGTCGGCATGAAGAAGAAGGGCGTCGACGAGTTCCCCTTCTGCATCCACCTCGTCTCCTGGGAGAAGGAAAACGTTTCCAGCGAGGCTCTCGAGGCGGCGCGAATTGCATGCAACAAGTACATGACCAAGTTCGCCGGCAAGGATACCTTCCACCTGAGGGTTAGGGTTCACCCCTTCCATGTCCTCCGAATCAACAAGATGCTTTCGTGCGCCGGAGCCGATAGGCTTCAGACTGGTATGCGCGGAGCTTTCGGGAAGCCGCTAGGTACTTGCGCTAGGGTTGCTATTGGACAGGTCCTGCTCTCTGTGCGTTGCAAGGAAAGCAACAAGAGCCACGCCGCTGAGGCCCTCCGCCGTGCCAAGTTCAAGTTTCCAGGCCGCCAAAAGATCATCGAGAGCAGAAAATGGGGATTCACCAAGTTCAACAGAACTGACTATGTGAGGTTGAAGTCTGAGGGTCGTATCATACCTGATGGTGTCAATGCCAAGCTTTTGGGATGCCATGGATCTTTGGCATTGCGTCGCCCTGGTGGAGCATTCATTGATGCTGCTGTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

222

Amino Acids

24.93

Weight (kDa)

10.43

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 12 - 166 1e-35 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 129
AccII CGCG 3 cut(s) 72, 205, 348
AclI AACGTT 1 cut(s) 177
AclWI GGATC 2 cut(s) 85, 619
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 105, 126
AfaI GTAC 2 cut(s) 224, 373
AfiI CCNNNNNNNGG 1 cut(s) 262
AgsI TTSAA 3 cut(s) 472, 529, 553
AjnI CCWGG 3 cut(s) 161, 478, 631
AluBI AGCT 2 cut(s) 353, 595
AluI AGCT 2 cut(s) 353, 595
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 2 cut(s) 85, 619
AlwNI CAGNNNCTG 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 2 cut(s) 450, 481
ApeKI GCWGC 1 cut(s) 653
AspLEI GCGC 4 cut(s) 205, 317, 348, 380
AspS9I GGNCC 2 cut(s) 400, 451
AsuHPI GGTGA 2 cut(s) 266, 511
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 400
AxyI CCTNAGG 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 16
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 640
BccI CCATC 1 cut(s) 572
BceAI ACGGC 1 cut(s) 444
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 195, 229
BciT130I CCWGG 3 cut(s) 163, 480, 633
BciVI GTATCC 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 3 cut(s) 18, 368, 381
BfuI GTATCC 1 cut(s) 241
BglI GCCNNNNNGGC 1 cut(s) 449
BisI GCNGC 6 cut(s) 70, 201, 365, 444, 484, 654
BlsI GCNGC 6 cut(s) 71, 202, 366, 445, 485, 655
Bme1390I CCNGG 3 cut(s) 163, 480, 633
Bme18I GGWCC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 400, 451
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 3 cut(s) 163, 480, 633
BmsI GCATC 5 cut(s) 11, 156, 294, 593, 640
BpiI GAAGAC 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 447
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 2 cut(s) 105, 126
BsaJI CCNNGG 4 cut(s) 70, 162, 607, 631
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 343
Bse21I CCTNAGG 1 cut(s) 260
Bse3DI GCAATG 1 cut(s) 620
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 3 cut(s) 163, 480, 633
BseDI CCNNGG 4 cut(s) 70, 162, 607, 631
BseGI GGATG 2 cut(s) 147, 608
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 3 cut(s) 251, 438, 549
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 1 cut(s) 640
Bsh1236I CGCG 3 cut(s) 72, 205, 348
BshFI GGCC 2 cut(s) 452, 483
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 2 cut(s) 240, 318
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 1 cut(s) 163
BsmBI CGTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 2 cut(s) 452, 483
BsoBI CYCGRG 1 cut(s) 194
Bsp143I GATC 4 cut(s) 33, 90, 492, 611
Bsp19I CCATGG 1 cut(s) 607
BspANI GGCC 2 cut(s) 452, 483
BspCNI CTCAG 3 cut(s) 252, 439, 550
BspFNI CGCG 3 cut(s) 72, 205, 348
BspLI GGNNCC 1 cut(s) 322
BspPI GGATC 2 cut(s) 85, 619
BsrDI GCAATG 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 4 cut(s) 70, 162, 607, 631
BssMI GATC 4 cut(s) 33, 90, 492, 611
BssNI GRCGYC 2 cut(s) 105, 126
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 3 cut(s) 163, 480, 633
BstACI GRCGYC 2 cut(s) 105, 126
BstC8I GCNNGC 2 cut(s) 214, 241
BstDEI CTNAG 3 cut(s) 260, 447, 558
BstDSI CCRYGG 2 cut(s) 70, 607
BstF5I GGATG 2 cut(s) 147, 608
BstFNI CGCG 3 cut(s) 72, 205, 348
BstHHI GCGC 4 cut(s) 205, 317, 348, 380
BstKTI GATC 4 cut(s) 36, 93, 495, 614
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 4 cut(s) 33, 90, 492, 611
BstMWI GCNNNNNNNGC 8 cut(s) 69, 197, 209, 386, 412, 426, 435, 449
BstNI CCWGG 3 cut(s) 163, 480, 633
BstNSI RCATGY 1 cut(s) 216
BstSCI CCNGG 3 cut(s) 161, 478, 631
BstUI CGCG 3 cut(s) 72, 205, 348
BstV1I GCAGC 1 cut(s) 640
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 2 cut(s) 90, 611
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 2 cut(s) 90, 611
Bsu36I CCTNAGG 1 cut(s) 260
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 452, 483
BtgI CCRYGG 2 cut(s) 70, 607
BtsCI GGATG 2 cut(s) 147, 608
Cac8I GCNNGC 2 cut(s) 214, 241
CaiI CAGNNNCTG 1 cut(s) 403
CfoI GCGC 4 cut(s) 205, 317, 348, 380
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 400, 451
Cfr42I CCGCGG 1 cut(s) 73
CseI GACGC 2 cut(s) 115, 614
Csp6I GTAC 2 cut(s) 223, 372
CviAII CATG 5 cut(s) 112, 213, 226, 284, 608
CviQI GTAC 2 cut(s) 223, 372
DdeI CTNAG 3 cut(s) 260, 447, 558
DpnI GATC 4 cut(s) 35, 92, 494, 613
DpnII GATC 4 cut(s) 33, 90, 492, 611
EciI GGCGGA 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 317
Eco81I CCTNAGG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 194
EcoO109I RGGNCCY 2 cut(s) 400, 451
EcoRII CCWGG 3 cut(s) 161, 478, 631
EcoT14I CCWWGG 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 607
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 5 cut(s) 115, 216, 229, 287, 611
FaiI YATR 9 cut(s) 102, 113, 214, 227, 285, 344, 543, 572, 609
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FatI CATG 5 cut(s) 111, 212, 225, 283, 607
FauI CCCGC 2 cut(s) 22, 68
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 6 cut(s) 70, 201, 365, 444, 484, 654
FokI GGATG 2 cut(s) 134, 615
Fsp4HI GCNGC 6 cut(s) 70, 201, 365, 444, 484, 654
FspBI CTAG 3 cut(s) 18, 368, 381
GlaI GCGC 4 cut(s) 204, 316, 347, 379
GluI GCNGC 6 cut(s) 70, 201, 365, 444, 484, 654
HaeIII GGCC 2 cut(s) 452, 483
HapII CCGG 2 cut(s) 240, 318
HgaI GACGC 2 cut(s) 115, 614
HhaI GCGC 4 cut(s) 205, 317, 348, 380
Hin1I GRCGYC 2 cut(s) 105, 126
Hin1II CATG 5 cut(s) 115, 216, 229, 287, 611
Hin6I GCGC 4 cut(s) 203, 315, 346, 378
HinP1I GCGC 4 cut(s) 203, 315, 346, 378
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 3 cut(s) 96, 294, 516
HpaII CCGG 2 cut(s) 240, 318
HphI GGTGA 2 cut(s) 266, 511
Hpy166II GTNNAC 3 cut(s) 130, 274, 661
Hpy188I TCNGA 4 cut(s) 95, 293, 336, 559
Hpy188III TCNNGA 4 cut(s) 37, 194, 358, 499
Hpy8I GTNNAC 3 cut(s) 130, 274, 661
Hpy99I CGWCG 4 cut(s) 110, 131, 134, 630
HpyAV CCTTC 5 cut(s) 115, 151, 163, 262, 289
HpyCH4IV ACGT 2 cut(s) 105, 177
HpyCH4V TGCA 4 cut(s) 147, 212, 216, 420
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 197, 209, 386, 412, 426, 435, 449
HpyF3I CTNAG 3 cut(s) 260, 447, 558
HpySE526I ACGT 2 cut(s) 105, 177
Hsp92I GRCGYC 2 cut(s) 105, 126
Hsp92II CATG 5 cut(s) 115, 216, 229, 287, 611
HspAI GCGC 4 cut(s) 203, 315, 346, 378
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 33, 90, 492, 611
LmnI GCTCC 3 cut(s) 320, 350, 638
Lsp1109I GCAGC 1 cut(s) 640
LweI GCATC 5 cut(s) 11, 156, 294, 593, 640
MaeI CTAG 3 cut(s) 18, 368, 381
MaeII ACGT 2 cut(s) 105, 177
MalI GATC 4 cut(s) 35, 92, 494, 613
MboI GATC 4 cut(s) 33, 90, 492, 611
MboII GAAGA 4 cut(s) 21, 100, 127, 130
MflI RGATCY 2 cut(s) 90, 611
MluCI AATT 1 cut(s) 207
MnlI CCTC 9 cut(s) 164, 181, 190, 255, 299, 442, 464, 540, 553
MroNI GCCGGC 1 cut(s) 239
MspA1I CMGCKG 2 cut(s) 72, 446
MspI CCGG 2 cut(s) 240, 318
MspR9I CCNGG 3 cut(s) 163, 480, 633
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 3 cut(s) 163, 480, 633
MvnI CGCG 3 cut(s) 72, 205, 348
MwoI GCNNNNNNNGC 8 cut(s) 69, 197, 209, 386, 412, 426, 435, 449
NaeI GCCGGC 1 cut(s) 241
NcoI CCATGG 1 cut(s) 607
NdeII GATC 4 cut(s) 33, 90, 492, 611
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 5 cut(s) 115, 216, 229, 287, 611
NlaIV GGNNCC 1 cut(s) 322
NspI RCATGY 1 cut(s) 216
PaeI GCATGC 1 cut(s) 216
PaeR7I CTCGAG 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 641
PdiI GCCGGC 1 cut(s) 241
PfeI GAWTC 3 cut(s) 96, 294, 516
PkrI GCNGC 6 cut(s) 71, 202, 366, 445, 485, 655
PpuMI RGGWCCY 1 cut(s) 400
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 3 cut(s) 161, 478, 631
PspGI CCWGG 3 cut(s) 161, 478, 631
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 2 cut(s) 400, 451
PspPPI RGGWCCY 1 cut(s) 400
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 90, 611
RsaI GTAC 2 cut(s) 224, 373
RsaNI GTAC 2 cut(s) 223, 372
SacII CCGCGG 1 cut(s) 73
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 6 cut(s) 70, 201, 365, 444, 484, 654
Sau3AI GATC 4 cut(s) 33, 90, 492, 611
Sau96I GGNCC 2 cut(s) 400, 451
ScrFI CCNGG 3 cut(s) 163, 480, 633
SfaNI GCATC 5 cut(s) 11, 156, 294, 593, 640
Sfr274I CTCGAG 1 cut(s) 194
Sfr303I CCGCGG 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 73
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 1 cut(s) 400
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SphI GCATGC 1 cut(s) 216
Sse9I AATT 1 cut(s) 207
SspMI CTAG 3 cut(s) 18, 368, 381
StyD4I CCNGG 3 cut(s) 161, 478, 631
StyI CCWWGG 1 cut(s) 607
TaiI ACGT 2 cut(s) 108, 180
TaqI TCGA 3 cut(s) 129, 195, 498
TasI AATT 1 cut(s) 207
TatI WGTACW 1 cut(s) 222
TauI GCSGC 5 cut(s) 72, 203, 367, 446, 486
TfiI GAWTC 3 cut(s) 96, 294, 516
TseI GCWGC 1 cut(s) 653
TspDTI ATGAA 2 cut(s) 128, 634
VpaK11BI GGWCC 1 cut(s) 400
XceI RCATGY 1 cut(s) 216
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 3 cut(s) 18, 368, 381
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.