pycom01g18330

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003864
Physical Location & Seq
Reverse (-)
17347000 .. 17348642
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18330.4

Sequence Viewer

Length: 675 bp
ATGGGGAGGAGGCCTGCGAGGTGCTATAGGCAGATTAAGAACAAGCCATACCCGAAATCGCGTTTCTGTCGCGGTGTCCCTGATCCCAAGATCAGAATTTATGATGTTGGAATGAAGAAGAGGGGTGTTGATGAGTTCCCCTTCTGTGTCCATCTTGTGAGTTGGGAGAAGGAGAATGTCTCGAGTGAGGCGCTTGAGGCAGCTCGCATCGCTTGCAACAAGTACATGACAAAGTATGCTGGGAAAGATACTTTCCATTTGAGAGTGAGGGTGCATCCGTTCCATGTTCTGCGCATCAACAAGATGCTTTCATGCGCTGGAGCTGATAGGCTCCAGACTGGAATGAGAGGTGCGTTTGGCAAGCCACTGGGAACATGTGCTCGAGTTGCTATCGGGCAAGTCCTCTTGTCTGTTCGCTGCAGGGATAGCAACAGTCAACATGCACAGGAGGCCCTCCGCCGCGCAAAGTTCAAGTTTCCTGGTCGCCAGAAGATTATTGTCAGCAGGAAGTGGGGTTTCACTAAGTTTAGCCGTGCTGATTATGTGAATTACAAGAAGGAAGGCCGAATCCAGCCAGATGGTGTTAACGCTAAGCTTCTTGGATGCCATGGTCCTCTGGCCAATCGTCAACCCGGAAAAGCATTCCTCACTCAACCTTCCTCATCGACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

225

Amino Acids

25.36

Weight (kDa)

10.45

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 293
AccII CGCG 3 cut(s) 61, 72, 462
AciI CCGC 3 cut(s) 72, 457, 460
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 1 cut(s) 96
AfaI GTAC 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 1 cut(s) 472
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 3 cut(s) 203, 323, 595
AluI AGCT 3 cut(s) 203, 323, 595
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 2 cut(s) 181, 381
AoxI GGCC 4 cut(s) 11, 450, 562, 618
ApeKI GCWGC 2 cut(s) 200, 417
ApoI RAATTY 1 cut(s) 96
AspLEI GCGC 4 cut(s) 193, 294, 317, 464
AspS9I GGNCC 2 cut(s) 451, 611
AsuC2I CCSGG 1 cut(s) 633
AvaI CYCGRG 2 cut(s) 181, 381
AvaII GGWCC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 212, 404
BccI CCATC 2 cut(s) 159, 572
BceAI ACGGC 1 cut(s) 516
BciT130I CCWGG 1 cut(s) 480
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 184
BfmI CTRYAG 2 cut(s) 25, 418
BfoI RGCGCY 1 cut(s) 194
BisI GCNGC 3 cut(s) 201, 418, 460
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 3 cut(s) 202, 419, 461
Bme1390I CCNGG 2 cut(s) 480, 633
Bme18I GGWCC 1 cut(s) 611
BmeT110I CYCGRG 2 cut(s) 181, 381
BmgT120I GGNCC 2 cut(s) 451, 611
BmiI GGNNCC 1 cut(s) 332
BmrFI CCNGG 2 cut(s) 480, 633
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 5 cut(s) 216, 283, 294, 303, 593
BmuI ACTGGG 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 2 cut(s) 317, 339
Bpu1102I GCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 633
BsaJI CCNNGG 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 343, 372
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 2 cut(s) 274, 608
BseNI ACTGG 2 cut(s) 343, 372
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 212, 404
BseYI CCCAGC 1 cut(s) 239
Bsh1236I CGCG 3 cut(s) 61, 72, 462
BshFI GGCC 4 cut(s) 13, 452, 564, 620
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 181, 381
BsiSI CCGG 1 cut(s) 633
BslFI GGGAC 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 4 cut(s) 13, 452, 564, 620
BsoBI CYCGRG 2 cut(s) 181, 381
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 2 cut(s) 82, 90
Bsp1720I GCTNAGC 1 cut(s) 591
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 3 cut(s) 72, 457, 460
BspANI GGCC 4 cut(s) 13, 452, 564, 620
BspFNI CGCG 3 cut(s) 61, 72, 462
BspLI GGNNCC 1 cut(s) 332
BspMAI CTGCAG 1 cut(s) 422
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 2 cut(s) 343, 372
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 2 cut(s) 82, 90
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 15, 205, 214, 362
BstDEI CTNAG 2 cut(s) 522, 591
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 2 cut(s) 274, 608
BstFNI CGCG 3 cut(s) 61, 72, 462
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 4 cut(s) 193, 294, 317, 464
BstKTI GATC 2 cut(s) 85, 93
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 2 cut(s) 82, 90
BstMWI GCNNNNNNNGC 6 cut(s) 197, 209, 213, 386, 426, 449
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 2 cut(s) 378, 443
BstSCI CCNGG 2 cut(s) 478, 631
BstSFI CTRYAG 2 cut(s) 25, 418
BstUI CGCG 3 cut(s) 61, 72, 462
BstV1I GCAGC 2 cut(s) 212, 404
BstXI CCANNNNNNTGG 1 cut(s) 578
BsuRI GGCC 4 cut(s) 13, 452, 564, 620
BtgI CCRYGG 1 cut(s) 607
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 274, 608
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 4 cut(s) 15, 205, 214, 362
CfoI GCGC 4 cut(s) 193, 294, 317, 464
Cfr13I GGNCC 2 cut(s) 451, 611
Csp6I GTAC 1 cut(s) 223
CviAII CATG 6 cut(s) 226, 284, 312, 375, 440, 608
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 522, 591
DpnI GATC 2 cut(s) 84, 92
DpnII GATC 2 cut(s) 82, 90
EaeI YGGCCR 1 cut(s) 618
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 607
Eco147I AGGCCT 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 611
Eco88I CYCGRG 2 cut(s) 181, 381
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 6 cut(s) 229, 287, 315, 378, 443, 611
FaqI GGGAC 1 cut(s) 62
FatI CATG 6 cut(s) 225, 283, 311, 374, 439, 607
Fnu4HI GCNGC 3 cut(s) 201, 418, 460
FokI GGATG 2 cut(s) 261, 615
Fsp4HI GCNGC 3 cut(s) 201, 418, 460
FspI TGCGCA 1 cut(s) 293
GlaI GCGC 4 cut(s) 192, 293, 316, 463
GluI GCNGC 3 cut(s) 201, 418, 460
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 2 cut(s) 317, 339
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 4 cut(s) 13, 452, 564, 620
HapII CCGG 1 cut(s) 633
HhaI GCGC 4 cut(s) 193, 294, 317, 464
Hin1II CATG 6 cut(s) 229, 287, 315, 378, 443, 611
Hin6I GCGC 4 cut(s) 191, 292, 315, 462
HinP1I GCGC 4 cut(s) 191, 292, 315, 462
HincII GTYRAC 3 cut(s) 437, 586, 629
HindII GTYRAC 3 cut(s) 437, 586, 629
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 1 cut(s) 567
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 1 cut(s) 633
Hpy166II GTNNAC 3 cut(s) 437, 586, 629
Hpy188I TCNGA 1 cut(s) 95
Hpy188III TCNNGA 2 cut(s) 181, 334
Hpy8I GTNNAC 3 cut(s) 437, 586, 629
HpyAV CCTTC 5 cut(s) 151, 163, 550, 554, 666
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4V TGCA 4 cut(s) 216, 274, 420, 443
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 209, 213, 386, 426, 449
HpyF3I CTNAG 2 cut(s) 522, 591
Hsp92II CATG 6 cut(s) 229, 287, 315, 378, 443, 611
HspAI GCGC 4 cut(s) 191, 292, 315, 462
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 2 cut(s) 82, 90
LmnI GCTCC 2 cut(s) 320, 336
Lsp1109I GCAGC 2 cut(s) 212, 404
LweI GCATC 5 cut(s) 216, 283, 294, 303, 593
MalI GATC 2 cut(s) 84, 92
MboI GATC 2 cut(s) 82, 90
MboII GAAGA 3 cut(s) 127, 130, 502
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 620
MluCI AATT 2 cut(s) 96, 547
MluNI TGGCCA 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 88
Mox20I TGGCCA 1 cut(s) 620
MscI TGGCCA 1 cut(s) 620
MseI TTAA 2 cut(s) 36, 585
Msp20I TGGCCA 1 cut(s) 620
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 2 cut(s) 480, 633
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 3 cut(s) 61, 72, 462
MwoI GCNNNNNNNGC 6 cut(s) 197, 209, 213, 386, 426, 449
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 607
NdeII GATC 2 cut(s) 82, 90
NlaIII CATG 6 cut(s) 229, 287, 315, 378, 443, 611
NlaIV GGNNCC 1 cut(s) 332
NsbI TGCGCA 1 cut(s) 293
NspI RCATGY 2 cut(s) 378, 443
PaeR7I CTCGAG 2 cut(s) 181, 381
PceI AGGCCT 1 cut(s) 13
PciI ACATGT 1 cut(s) 374
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 567
PkrI GCNGC 3 cut(s) 202, 419, 461
PscI ACATGT 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 478
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 332
PspPI GGNCC 2 cut(s) 451, 611
PspXI VCTCGAGB 1 cut(s) 381
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PstI CTGCAG 1 cut(s) 422
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SaqAI TTAA 2 cut(s) 36, 585
SatI GCNGC 3 cut(s) 201, 418, 460
Sau3AI GATC 2 cut(s) 82, 90
Sau96I GGNCC 2 cut(s) 451, 611
ScrFI CCNGG 2 cut(s) 480, 633
SduI GDGCHC 1 cut(s) 382
SetI ASST 6 cut(s) 23, 205, 325, 352, 597, 658
SfaNI GCATC 5 cut(s) 216, 283, 294, 303, 593
SfcI CTRYAG 2 cut(s) 25, 418
Sfr274I CTCGAG 2 cut(s) 181, 381
SinI GGWCC 1 cut(s) 611
SlaI CTCGAG 2 cut(s) 181, 381
SmlI CTYRAG 3 cut(s) 181, 194, 381
SmoI CTYRAG 3 cut(s) 181, 194, 381
Sse9I AATT 2 cut(s) 96, 547
SseBI AGGCCT 1 cut(s) 13
SsiI CCGC 3 cut(s) 72, 457, 460
StuI AGGCCT 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 478, 631
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 182, 382, 665
TasI AATT 2 cut(s) 96, 547
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 2 cut(s) 36, 585
Tru9I TTAA 2 cut(s) 36, 585
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 200, 417
TspDTI ATGAA 2 cut(s) 128, 300
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 372
VpaK11BI GGWCC 1 cut(s) 611
XapI RAATTY 1 cut(s) 96
XceI RCATGY 2 cut(s) 378, 443
XhoI CTCGAG 2 cut(s) 181, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.