RchiOBHm_Chr5g0073671

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003864
Physical Location & Seq
Forward (+)
79726680 .. 79728412
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34855

Sequence Viewer

Length: 663 bp
ATGGGTCGCCGCCCCGCCCGTTGCTACCGCCAGATCAAGAACAAGCCGTACCCGAAGTCCCGCTTCTGCCGCGGTGTGCCGGACCCGAAGATCCGAATCTACGACGTCGGCATGAAGAAGAAGGGCGTCGACGAGTTCCCCTTCTGCGTCCACCTCGTCTCCTGGGAGAAGGAGAACGTCTCCAGCGAGGCGCTCGAGGCGGCGCGTATCGCCTGCAACAAGTACATGGCCAAGTTCGCCGGCAAGGACGCGTTCCATCTACGCGTTAGGGTTCACCCGTTCCATGTGCTCAGGATTAACAAGATGCTTTCCTGCGCCGGAGCCGATAGGCTTCAGACCGGTATGAGGGGAGCGTTCGGGAAGCCTCTTGGGACTTGCGCTAGGGTTGATATTGGGCAGGTGCTGCTCTCTGTGAGGTGCAAGGACAGCAACAAGAACAATGCTTGTGAGGCTCTGAGGAGGGCTAAGTTCAAGTTTCCCGGTCGCCAAAAGATCATTGAGAGCAGGAAGTGGGGATTCACCAAGTTCAACCGTGTTGACTATGTAAGATTGAAGTCAGAGGCACGCATTCTTCCTGATGGTGTCAATGCCAAGCTCTTGGGATGCCACGGATCTTTGGCTTTGCGTAAGCCTGGTGAAGCTTTCATTGACGCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16

Protein Analysis

220

Amino Acids

24.84

Weight (kDa)

10.2

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 12 - 166 4.1e-35 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
AatII GACGTC 1 cut(s) 108
Acc36I ACCTGC 1 cut(s) 388
AccI GTMKAC 1 cut(s) 129
AccII CGCG 4 cut(s) 72, 205, 251, 264
AciI CCGC 7 cut(s) 10, 15, 28, 61, 70, 72, 200
AclWI GGATC 2 cut(s) 85, 619
AcoI YGGCCR 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 105, 126
AfaI GTAC 2 cut(s) 50, 224
AfiI CCNNNNNNNGG 1 cut(s) 345
AflIII ACRYGT 2 cut(s) 249, 262
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 3 cut(s) 472, 529, 553
AjnI CCWGG 2 cut(s) 161, 631
AluBI AGCT 2 cut(s) 595, 641
AluI AGCT 2 cut(s) 595, 641
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 2 cut(s) 163, 184
AlwI GGATC 2 cut(s) 85, 619
AlwNI CAGNNNCTG 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 3 cut(s) 403, 653, 656
AsiGI ACCGGT 1 cut(s) 338
AspLEI GCGC 4 cut(s) 193, 205, 317, 380
AspS9I GGNCC 1 cut(s) 82
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 3 cut(s) 266, 511, 647
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 390, 640, 643
BccI CCATC 2 cut(s) 264, 572
BceAI ACGGC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 163, 633
BcnI CCSGG 1 cut(s) 480
BcoDI GTCTC 2 cut(s) 163, 184
BfaI CTAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 194
BfuAI ACCTGC 1 cut(s) 388
BisI GCNGC 6 cut(s) 10, 70, 201, 404, 654, 657
BlsI GCNGC 6 cut(s) 11, 71, 202, 405, 655, 658
Bme1390I CCNGG 3 cut(s) 163, 480, 633
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 84, 322
BmrFI CCNGG 3 cut(s) 163, 480, 633
BmsI GCATC 2 cut(s) 294, 593
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 480
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 2 cut(s) 105, 126
BsaJI CCNNGG 3 cut(s) 70, 162, 607
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse118I RCCGGY 2 cut(s) 239, 338
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 2 cut(s) 163, 633
BseDI CCNNGG 3 cut(s) 70, 162, 607
BseGI GGATG 1 cut(s) 608
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 304, 446
BseRI GAGGAG 1 cut(s) 472
BseXI GCAGC 3 cut(s) 390, 640, 643
Bsh1236I CGCG 4 cut(s) 72, 205, 251, 264
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 1 cut(s) 230
BshTI ACCGGT 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 5 cut(s) 80, 240, 318, 339, 480
BslFI GGGAC 2 cut(s) 43, 385
BslI CCNNNNNNNGG 1 cut(s) 345
BsmAI GTCTC 2 cut(s) 163, 184
BsmBI CGTCTC 2 cut(s) 163, 184
BsmFI GGGAC 2 cut(s) 43, 385
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 4 cut(s) 33, 90, 492, 611
BspACI CCGC 7 cut(s) 10, 15, 28, 61, 70, 72, 200
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 2 cut(s) 303, 447
BspFNI CGCG 4 cut(s) 72, 205, 251, 264
BspLI GGNNCC 2 cut(s) 84, 322
BspMI ACCTGC 1 cut(s) 388
BspPI GGATC 2 cut(s) 85, 619
BsrFI RCCGGY 2 cut(s) 239, 338
BssAI RCCGGY 2 cut(s) 239, 338
BssECI CCNNGG 3 cut(s) 70, 162, 607
BssMI GATC 4 cut(s) 33, 90, 492, 611
BssNI GRCGYC 2 cut(s) 105, 126
Bst2UI CCWGG 2 cut(s) 163, 633
Bst4CI ACNGT 1 cut(s) 533
BstACI GRCGYC 2 cut(s) 105, 126
BstAPI GCANNNNNTGC 1 cut(s) 403
BstC8I GCNNGC 3 cut(s) 214, 241, 565
BstDEI CTNAG 4 cut(s) 290, 455, 465, 660
BstDSI CCRYGG 2 cut(s) 70, 607
BstF5I GGATG 1 cut(s) 608
BstFNI CGCG 4 cut(s) 72, 205, 251, 264
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 4 cut(s) 193, 205, 317, 380
BstKTI GATC 4 cut(s) 36, 93, 495, 614
BstMAI GTCTC 2 cut(s) 163, 184
BstMBI GATC 4 cut(s) 33, 90, 492, 611
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 7 cut(s) 69, 197, 209, 236, 403, 426, 449
BstNI CCWGG 2 cut(s) 163, 633
BstSCI CCNGG 3 cut(s) 161, 478, 631
BstUI CGCG 4 cut(s) 72, 205, 251, 264
BstV1I GCAGC 3 cut(s) 390, 640, 643
BstX2I RGATCY 2 cut(s) 90, 611
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 2 cut(s) 90, 611
BsuRI GGCC 1 cut(s) 230
BtgI CCRYGG 2 cut(s) 70, 607
BtsCI GGATG 1 cut(s) 608
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 3 cut(s) 214, 241, 565
CaiI CAGNNNCTG 1 cut(s) 403
CfoI GCGC 4 cut(s) 193, 205, 317, 380
Cfr10I RCCGGY 2 cut(s) 239, 338
Cfr13I GGNCC 1 cut(s) 82
Cfr42I CCGCGG 1 cut(s) 73
CseI GACGC 3 cut(s) 115, 136, 257
Csp6I GTAC 2 cut(s) 49, 223
CspAI ACCGGT 1 cut(s) 338
CviAII CATG 3 cut(s) 112, 226, 284
CviQI GTAC 2 cut(s) 49, 223
DdeI CTNAG 4 cut(s) 290, 455, 465, 660
DpnI GATC 4 cut(s) 35, 92, 494, 613
DpnII GATC 4 cut(s) 33, 90, 492, 611
EaeI YGGCCR 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 161, 631
Esp3I CGTCTC 2 cut(s) 163, 184
FaeI CATG 3 cut(s) 115, 229, 287
FaiI YATR 5 cut(s) 113, 227, 285, 344, 543
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 2 cut(s) 43, 385
FatI CATG 3 cut(s) 111, 225, 283
FauI CCCGC 2 cut(s) 22, 68
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 6 cut(s) 10, 70, 201, 404, 654, 657
FokI GGATG 1 cut(s) 615
Fsp4HI GCNGC 6 cut(s) 10, 70, 201, 404, 654, 657
FspBI CTAG 1 cut(s) 381
GlaI GCGC 4 cut(s) 192, 204, 316, 379
GluI GCNGC 6 cut(s) 10, 70, 201, 404, 654, 657
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 1 cut(s) 230
HapII CCGG 5 cut(s) 80, 240, 318, 339, 480
HgaI GACGC 3 cut(s) 115, 136, 257
HhaI GCGC 4 cut(s) 193, 205, 317, 380
Hin1I GRCGYC 2 cut(s) 105, 126
Hin1II CATG 3 cut(s) 115, 229, 287
Hin6I GCGC 4 cut(s) 191, 203, 315, 378
HinP1I GCGC 4 cut(s) 191, 203, 315, 378
HincII GTYRAC 2 cut(s) 130, 538
HindII GTYRAC 2 cut(s) 130, 538
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 2 cut(s) 96, 516
HpaII CCGG 5 cut(s) 80, 240, 318, 339, 480
HphI GGTGA 3 cut(s) 266, 511, 647
Hpy166II GTNNAC 4 cut(s) 130, 151, 274, 538
Hpy188I TCNGA 4 cut(s) 95, 336, 456, 559
Hpy188III TCNNGA 4 cut(s) 37, 292, 358, 575
Hpy8I GTNNAC 4 cut(s) 130, 151, 274, 538
Hpy99I CGWCG 4 cut(s) 107, 110, 131, 134
HpyAV CCTTC 3 cut(s) 115, 151, 163
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4IV ACGT 2 cut(s) 105, 177
HpyCH4V TGCA 2 cut(s) 216, 420
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 197, 209, 236, 403, 426, 449
HpyF3I CTNAG 4 cut(s) 290, 455, 465, 660
HpySE526I ACGT 2 cut(s) 105, 177
Hsp92I GRCGYC 2 cut(s) 105, 126
Hsp92II CATG 3 cut(s) 115, 229, 287
HspAI GCGC 4 cut(s) 191, 203, 315, 378
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 33, 90, 492, 611
LmnI GCTCC 2 cut(s) 320, 350
Lsp1109I GCAGC 3 cut(s) 390, 640, 643
LweI GCATC 2 cut(s) 294, 593
MaeI CTAG 1 cut(s) 381
MaeII ACGT 2 cut(s) 105, 177
MalI GATC 4 cut(s) 35, 92, 494, 613
MboI GATC 4 cut(s) 33, 90, 492, 611
MboII GAAGA 4 cut(s) 100, 127, 130, 563
MflI RGATCY 2 cut(s) 90, 611
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 1 cut(s) 230
MluI ACGCGT 2 cut(s) 249, 262
MluNI TGGCCA 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 230
MroNI GCCGGC 1 cut(s) 239
MscI TGGCCA 1 cut(s) 230
MseI TTAA 1 cut(s) 297
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 5 cut(s) 80, 240, 318, 339, 480
MspR9I CCNGG 3 cut(s) 163, 480, 633
Mva1269I GAATGC 1 cut(s) 567
MvaI CCWGG 2 cut(s) 163, 633
MvnI CGCG 4 cut(s) 72, 205, 251, 264
MwoI GCNNNNNNNGC 7 cut(s) 69, 197, 209, 236, 403, 426, 449
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 480
NdeII GATC 4 cut(s) 33, 90, 492, 611
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 3 cut(s) 115, 229, 287
NlaIV GGNNCC 2 cut(s) 84, 322
PaeR7I CTCGAG 1 cut(s) 194
PaqCI CACCTGC 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 567
PdiI GCCGGC 1 cut(s) 241
PfeI GAWTC 2 cut(s) 96, 516
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 6 cut(s) 11, 71, 202, 405, 655, 658
Psp6I CCWGG 2 cut(s) 161, 631
PspGI CCWGG 2 cut(s) 161, 631
PspN4I GGNNCC 2 cut(s) 84, 322
PspPI GGNCC 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 194
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 90, 611
RsaI GTAC 2 cut(s) 50, 224
RsaNI GTAC 2 cut(s) 49, 223
SacII CCGCGG 1 cut(s) 73
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 6 cut(s) 10, 70, 201, 404, 654, 657
Sau3AI GATC 4 cut(s) 33, 90, 492, 611
Sau96I GGNCC 1 cut(s) 82
ScrFI CCNGG 3 cut(s) 163, 480, 633
SduI GDGCHC 1 cut(s) 291
SetI ASST 7 cut(s) 108, 156, 180, 402, 419, 597, 643
SfaNI GCATC 2 cut(s) 294, 593
Sfr274I CTCGAG 1 cut(s) 194
Sfr303I CCGCGG 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 73
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 1 cut(s) 82
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SsiI CCGC 7 cut(s) 10, 15, 28, 61, 70, 72, 200
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 161, 478, 631
TaaI ACNGT 1 cut(s) 533
TaiI ACGT 2 cut(s) 108, 180
TaqI TCGA 2 cut(s) 129, 195
TatI WGTACW 1 cut(s) 222
TauI GCSGC 3 cut(s) 12, 72, 203
TfiI GAWTC 2 cut(s) 96, 516
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TseI GCWGC 3 cut(s) 403, 653, 656
TspDTI ATGAA 2 cut(s) 128, 634
TspGWI ACGGA 1 cut(s) 624
VpaK11BI GGWCC 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 1 cut(s) 381
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.