RLG00000026878

Hydroxyacylglutathione hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017173
Physical Location & Seq
Reverse (-)
6729727 .. 6732056
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026878

Sequence Viewer

Length: 777 bp
ATGAAAATCCATCACGTTGCTTGCTTGGAAGACAATTACGCATACCTAATTATTGACGAGAGTACCAAAGAAGCCGCGGTTGTGGATCCTGTTGAACCCGAAAAGGTTCTTAAAGCAGCCCAAGACCACGGTGCCCAAGTCAAACTCGTCCTCACCACTCACCACCATTGGGATCATGCCGGAGGAAATGAGAAGATAAAGCAGTTGGTGCCTGGCATTAAAGTGTATGGGGGTTCTGTTGATAATGTGAAGGGCTGCACTGACAAGGTGGACAATGGTGATAAGATTTCGCTCGGAGCTGATACTCAAATACTCTCGCTTCACACACCTTGTCACACAAAAGGTCACATAAGTTATTACGTGACAGGCAAAGAGGGAGAGGAACCAGCTGTTTTCACCGGAGACACACTGTTTATTGCTGGTTGCGGCAAATTTTTCGAAGGAACAGCTGAACAGATGCATCAGTCACTCTGCGTAACTCTAGCTTCATTGCCAAAGCCTACACAAGTTTACTGTGGTCATGAGTACACAGTGAAGAACTTGCAATTTGCTCAGACAGTTGAACCAGACAATGCCAAGATAAAGCGGAAGTTATCCTGGGCACAGCAGCAGCGGGAAGCAGGTCTCCCGACAGTTCCCTCAACCATTGAGAGTGAGATGGAAACAAACCCTTTTGTGCGGGCTGATCTGCCAGAGCTTCAGGAGAAGGTCGGTTGCCACAATGCTGTGGACGCCCTACGTGAGATAAGGCAGAGGAAGGACAATTGGAGGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
HAGH_C Lactamase_B

Protein Analysis

259

Amino Acids

28.52

Weight (kDa)

6.0

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 13 - 174 2.3e-13 Metallo-beta-lactamase superfamily
HAGH_C PF16123 175 - 256 1.3e-28 Hydroxyacylglutathione hydrolase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10850
fragaria_vesca FvH4_7g26250 FvH4_7g26250
malus_domestica MD01G1171200.v1.1
prunus_persica Prupe.2G266700_v2.0.a1
pyrus_communis pycom01g18340
rosa_chinensis RchiOBHm_Chr1g0372701
rosa_laevigata RLG00000026878
rosa_multiflora Rmu_sc0034231.1_g000002
rosa_roxburghii Rroxscaffold_4G00284740
rosa_rugosa Rorug01G0373800
rosa_samantha Rh1BG346000
rosa_wichuraiana Rw1G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 131, 208
AccII CGCG 1 cut(s) 77
AciI CCGC 6 cut(s) 75, 77, 426, 586, 613, 679
AclWI GGATC 3 cut(s) 80, 93, 180
AcsI RAATTY 1 cut(s) 431
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 732
AfaI GTAC 2 cut(s) 64, 527
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 95, 563
AjnI CCWGG 2 cut(s) 211, 596
AluBI AGCT 5 cut(s) 299, 389, 449, 485, 697
AluI AGCT 5 cut(s) 299, 389, 449, 485, 697
Alw26I GTCTC 2 cut(s) 396, 629
AlwI GGATC 3 cut(s) 80, 93, 180
ApeKI GCWGC 4 cut(s) 116, 255, 607, 610
ApoI RAATTY 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 105
AsuHPI GGTGA 4 cut(s) 145, 152, 290, 388
AsuII TTCGAA 1 cut(s) 438
BaeGI GKGCMC 2 cut(s) 136, 604
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 85
BanI GGYRCC 2 cut(s) 131, 208
BbsI GAAGAC 1 cut(s) 36
BbvI GCAGC 4 cut(s) 128, 242, 619, 622
BccI CCATC 2 cut(s) 18, 652
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 2 cut(s) 213, 598
BcoDI GTCTC 2 cut(s) 396, 629
BfaI CTAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 611
BisI GCNGC 6 cut(s) 75, 117, 256, 427, 608, 611
BlsI GCNGC 6 cut(s) 76, 118, 257, 428, 609, 612
Bme1390I CCNGG 2 cut(s) 213, 598
BmiI GGNNCC 4 cut(s) 87, 133, 210, 384
BmrFI CCNGG 2 cut(s) 213, 598
BmsI GCATC 2 cut(s) 447, 469
BpiI GAAGAC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 438
BsaAI YACGTR 2 cut(s) 361, 740
BsaHI GRCGYC 1 cut(s) 732
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 75, 127, 597
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 488
BseBI CCWGG 2 cut(s) 213, 598
BseDI CCNNGG 3 cut(s) 75, 127, 597
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 566
BseSI GKGCMC 2 cut(s) 136, 604
BseXI GCAGC 4 cut(s) 128, 242, 619, 622
BsgI GTGCAG 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 77
BshNI GGYRCC 2 cut(s) 131, 208
BsiSI CCGG 2 cut(s) 180, 399
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 2 cut(s) 396, 629
Bso31I GGTCTC 1 cut(s) 629
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 136, 604
Bsp143I GATC 3 cut(s) 85, 172, 685
BspACI CCGC 6 cut(s) 75, 77, 426, 586, 613, 679
BspCNI CTCAG 1 cut(s) 565
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 4 cut(s) 87, 133, 210, 384
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 3 cut(s) 80, 93, 180
BspT104I TTCGAA 1 cut(s) 438
BspT107I GGYRCC 2 cut(s) 131, 208
BspTNI GGTCTC 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 488
BssECI CCNNGG 3 cut(s) 75, 127, 597
BssMI GATC 3 cut(s) 85, 172, 685
BssNI GRCGYC 1 cut(s) 732
Bst2UI CCWGG 2 cut(s) 213, 598
Bst4CI ACNGT 6 cut(s) 131, 411, 515, 532, 559, 634
BstACI GRCGYC 1 cut(s) 732
BstAPI GCANNNNNTGC 1 cut(s) 208
BstBAI YACGTR 2 cut(s) 361, 740
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 2 cut(s) 22, 681
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 2 cut(s) 75, 127
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 3 cut(s) 88, 175, 688
BstMAI GTCTC 2 cut(s) 396, 629
BstMBI GATC 3 cut(s) 85, 172, 685
BstMWI GCNNNNNNNGC 2 cut(s) 208, 731
BstNI CCWGG 2 cut(s) 213, 598
BstSCI CCNGG 2 cut(s) 211, 596
BstSLI GKGCMC 2 cut(s) 136, 604
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 4 cut(s) 128, 242, 619, 622
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BtgI CCRYGG 2 cut(s) 75, 127
BtsIMutI CAGTG 3 cut(s) 258, 407, 537
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 2 cut(s) 22, 681
CciI TCATGA 1 cut(s) 520
Cfr42I CCGCGG 1 cut(s) 78
CseI GACGC 1 cut(s) 740
Csp6I GTAC 2 cut(s) 63, 526
CviAII CATG 2 cut(s) 176, 521
CviQI GTAC 2 cut(s) 63, 526
DdeI CTNAG 1 cut(s) 552
DpnI GATC 3 cut(s) 87, 174, 687
DpnII GATC 3 cut(s) 85, 172, 685
Eco31I GGTCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 683
EcoRII CCWGG 2 cut(s) 211, 596
EcoT22I ATGCAT 1 cut(s) 462
FaeI CATG 2 cut(s) 179, 524
FaiI YATR 5 cut(s) 43, 177, 228, 350, 522
FatI CATG 2 cut(s) 175, 520
FauI CCCGC 2 cut(s) 606, 672
Fnu4HI GCNGC 6 cut(s) 75, 117, 256, 427, 608, 611
Fsp4HI GCNGC 6 cut(s) 75, 117, 256, 427, 608, 611
FspBI CTAG 1 cut(s) 482
GluI GCNGC 6 cut(s) 75, 117, 256, 427, 608, 611
HapII CCGG 2 cut(s) 180, 399
HgaI GACGC 1 cut(s) 740
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 2 cut(s) 179, 524
HpaII CCGG 2 cut(s) 180, 399
HphI GGTGA 4 cut(s) 145, 152, 290, 388
Hpy166II GTNNAC 4 cut(s) 271, 511, 528, 730
Hpy188I TCNGA 2 cut(s) 296, 555
Hpy188III TCNNGA 3 cut(s) 521, 628, 701
Hpy8I GTNNAC 4 cut(s) 271, 511, 528, 730
HpyAV CCTTC 4 cut(s) 244, 434, 700, 751
HpyCH4III ACNGT 6 cut(s) 131, 411, 515, 532, 559, 634
HpyCH4IV ACGT 3 cut(s) 15, 360, 739
HpyCH4V TGCA 3 cut(s) 258, 460, 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 731
HpyF3I CTNAG 1 cut(s) 552
HpySE526I ACGT 3 cut(s) 15, 360, 739
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 2 cut(s) 179, 524
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 3 cut(s) 85, 172, 685
LmnI GCTCC 1 cut(s) 296
Lsp1109I GCAGC 4 cut(s) 128, 242, 619, 622
LweI GCATC 2 cut(s) 447, 469
MaeI CTAG 1 cut(s) 482
MaeII ACGT 3 cut(s) 15, 360, 739
MaeIII GTNAC 5 cut(s) 332, 344, 361, 465, 475
MalI GATC 3 cut(s) 87, 174, 687
MboI GATC 3 cut(s) 85, 172, 685
MboII GAAGA 3 cut(s) 41, 205, 547
MfeI CAATTG 1 cut(s) 763
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 2 cut(s) 136, 604
MluCI AATT 5 cut(s) 34, 48, 431, 545, 763
MnlI CCTC 7 cut(s) 161, 176, 367, 373, 649, 747, 762
Mph1103I ATGCAT 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 2 cut(s) 111, 219
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 4 cut(s) 77, 389, 449, 613
MspI CCGG 2 cut(s) 180, 399
MspR9I CCNGG 2 cut(s) 213, 598
MunI CAATTG 1 cut(s) 763
MvaI CCWGG 2 cut(s) 213, 598
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 208, 731
NdeII GATC 3 cut(s) 85, 172, 685
NlaIII CATG 2 cut(s) 179, 524
NlaIV GGNNCC 4 cut(s) 87, 133, 210, 384
NmuCI GTSAC 4 cut(s) 332, 344, 361, 465
NsiI ATGCAT 1 cut(s) 462
NspV TTCGAA 1 cut(s) 438
PagI TCATGA 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 105
PkrI GCNGC 6 cut(s) 76, 118, 257, 428, 609, 612
Ppu21I YACGTR 2 cut(s) 361, 740
Psp6I CCWGG 2 cut(s) 211, 596
PspGI CCWGG 2 cut(s) 211, 596
PspN4I GGNNCC 4 cut(s) 87, 133, 210, 384
PsuI RGATCY 1 cut(s) 85
PvuII CAGCTG 2 cut(s) 389, 449
RsaI GTAC 2 cut(s) 64, 527
RsaNI GTAC 2 cut(s) 63, 526
RseI CAYNNNNRTG 1 cut(s) 221
SacII CCGCGG 1 cut(s) 78
SaqAI TTAA 2 cut(s) 111, 219
SatI GCNGC 6 cut(s) 75, 117, 256, 427, 608, 611
Sau3AI GATC 3 cut(s) 85, 172, 685
ScrFI CCNGG 2 cut(s) 213, 598
SduI GDGCHC 2 cut(s) 136, 604
SfaNI GCATC 2 cut(s) 447, 469
Sfr303I CCGCGG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 438
SgrBI CCGCGG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 221
Sse9I AATT 5 cut(s) 34, 48, 431, 545, 763
SsiI CCGC 6 cut(s) 75, 77, 426, 586, 613, 679
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 2 cut(s) 211, 596
TaaI ACNGT 6 cut(s) 131, 411, 515, 532, 559, 634
TaiI ACGT 3 cut(s) 18, 363, 742
TaqI TCGA 1 cut(s) 438
TasI AATT 5 cut(s) 34, 48, 431, 545, 763
TatI WGTACW 1 cut(s) 525
TauI GCSGC 2 cut(s) 77, 429
Tru1I TTAA 2 cut(s) 111, 219
Tru9I TTAA 2 cut(s) 111, 219
TscAI CASTG 3 cut(s) 265, 414, 537
TseFI GTSAC 4 cut(s) 332, 344, 361, 465
TseI GCWGC 4 cut(s) 116, 255, 607, 610
Tsp45I GTSAC 4 cut(s) 332, 344, 361, 465
TspDTI ATGAA 2 cut(s) 17, 477
TspRI CASTG 3 cut(s) 265, 414, 537
XapI RAATTY 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.