MD01G1210300.v1.1

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010215
Physical Location & Seq
Forward (+)
30418498 .. 30420067
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1210300.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTTCCTGCGAGCGATCGGACGGCCGTTGTTGGCCAGGGTGAAGCAGACGACAGGGATTGTAGGGCTGGACGTGGTCCCCAATGCGAGGGAGGTCCTAATCGAGCTCTACTCCAAAACCCTAAAGGAGGTCCAGGCCATCCCCGAGGACGAAGGCTACCGCAAGGCCGTCGAGAGCTTCACGCGCCACCGCCTCAATGTCTGCCGCGAAGAGGAGGACTGGGAGGCCATCGAGAAGAAGCTCGGCTGCGGCCAGGTCGAGGAGCTCATCGAGGAGGCGCGCGACGAGCTCACTCTCACCGGCAAGATGATCGAGTGGGATCCATGGGGTGTTCCTGATGATTACGAATGTGAGGTGATCGAGAATGATGCTCCAGTTCCCAAGCACGTTCCTCTGCACCGACCTGGTCCTCTTCCTGAGGAGTTTTACAAAACGCTGGAAGGCCTTGCTACAAACCAACCAAAGTTGGATGAGGCTAAAGTCACCTCTATTGGTTCAGACGCAAAGGAGTAA

Protein Analysis

171

Amino Acids

19.26

Weight (kDa)

4.77

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETC_C1_NDUFA5 PF04716 22 - 88 4.1e-29 ETC complex I subunit conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 184, 207, 280, 282
AciI CCGC 4 cut(s) 160, 190, 205, 249
AclWI GGATC 2 cut(s) 314, 327
AcoI YGGCCR 3 cut(s) 23, 33, 250
AfiI CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 4 cut(s) 35, 132, 252, 403
AluBI AGCT 5 cut(s) 106, 177, 241, 265, 289
AluI AGCT 5 cut(s) 106, 177, 241, 265, 289
Alw21I GWGCWC 3 cut(s) 108, 267, 291
AlwI GGATC 2 cut(s) 314, 327
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 7 cut(s) 23, 33, 135, 165, 225, 250, 442
ApeKI GCWGC 1 cut(s) 246
AspLEI GCGC 3 cut(s) 186, 280, 282
AspS9I GGNCC 4 cut(s) 76, 94, 130, 407
AsuHPI GGTGA 4 cut(s) 52, 289, 367, 475
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 4 cut(s) 76, 94, 130, 407
AxyI CCTNAGG 1 cut(s) 417
BalI TGGCCA 1 cut(s) 35
BamHI GGATCC 1 cut(s) 319
BanII GRGCYC 3 cut(s) 108, 267, 291
Bbv12I GWGCWC 3 cut(s) 108, 267, 291
BbvI GCAGC 1 cut(s) 233
BccI CCATC 2 cut(s) 146, 236
BceAI ACGGC 3 cut(s) 10, 38, 152
BcgI CGANNNNNNTGC 6 cut(s) 151, 185, 292, 326, 350, 384
BciT130I CCWGG 4 cut(s) 37, 134, 254, 405
BisI GCNGC 3 cut(s) 205, 247, 250
BlsI GCNGC 3 cut(s) 206, 248, 251
Bme1390I CCNGG 4 cut(s) 37, 134, 254, 405
Bme18I GGWCC 4 cut(s) 76, 94, 130, 407
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 4 cut(s) 76, 94, 130, 407
BmiI GGNNCC 2 cut(s) 78, 321
BmrFI CCNGG 4 cut(s) 37, 134, 254, 405
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 36, 144, 323
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 224, 374
Bse21I CCTNAGG 1 cut(s) 417
BseBI CCWGG 4 cut(s) 37, 134, 254, 405
BseDI CCNNGG 3 cut(s) 36, 144, 323
BseGI GGATG 2 cut(s) 138, 475
BseLI CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 224, 374
BsePI GCGCGC 1 cut(s) 278
BseRI GAGGAG 4 cut(s) 227, 275, 287, 434
BseX3I CGGCCG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 233
BsgI GTGCAG 1 cut(s) 380
Bsh1236I CGCG 4 cut(s) 184, 207, 280, 282
Bsh1285I CGRYCG 2 cut(s) 18, 26
BshFI GGCC 7 cut(s) 25, 35, 137, 167, 227, 252, 444
BsiEI CGRYCG 2 cut(s) 18, 26
BsiHKAI GWGCWC 3 cut(s) 108, 267, 291
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 7 cut(s) 25, 35, 137, 167, 227, 252, 444
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 3 cut(s) 108, 267, 291
Bsp143I GATC 4 cut(s) 15, 309, 319, 357
Bsp19I CCATGG 1 cut(s) 323
BspACI CCGC 4 cut(s) 160, 190, 205, 249
BspANI GGCC 7 cut(s) 25, 35, 137, 167, 227, 252, 444
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 4 cut(s) 184, 207, 280, 282
BspLI GGNNCC 2 cut(s) 78, 321
BspPI GGATC 2 cut(s) 314, 327
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 2 cut(s) 224, 374
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 36, 144, 323
BssHII GCGCGC 1 cut(s) 278
BssMI GATC 4 cut(s) 15, 309, 319, 357
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 4 cut(s) 37, 134, 254, 405
Bst6I CTCTTC 2 cut(s) 204, 417
BstC8I GCNNGC 2 cut(s) 12, 280
BstDEI CTNAG 1 cut(s) 417
BstDSI CCRYGG 1 cut(s) 323
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 138, 475
BstFNI CGCG 4 cut(s) 184, 207, 280, 282
BstHHI GCGC 3 cut(s) 186, 280, 282
BstKTI GATC 4 cut(s) 18, 312, 322, 360
BstMBI GATC 4 cut(s) 15, 309, 319, 357
BstMCI CGRYCG 2 cut(s) 18, 26
BstMWI GCNNNNNNNGC 2 cut(s) 183, 286
BstNI CCWGG 4 cut(s) 37, 134, 254, 405
BstSCI CCNGG 4 cut(s) 35, 132, 252, 403
BstUI CGCG 4 cut(s) 184, 207, 280, 282
BstV1I GCAGC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BstZI CGGCCG 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 7 cut(s) 25, 35, 137, 167, 227, 252, 444
BtgI CCRYGG 1 cut(s) 323
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 2 cut(s) 138, 475
Cac8I GCNNGC 2 cut(s) 12, 280
CfoI GCGC 3 cut(s) 186, 280, 282
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 4 cut(s) 76, 94, 130, 407
CsiI ACCWGGT 1 cut(s) 403
CviAII CATG 1 cut(s) 324
DdeI CTNAG 1 cut(s) 417
DpnI GATC 4 cut(s) 17, 311, 321, 359
DpnII GATC 4 cut(s) 15, 309, 319, 357
EaeI YGGCCR 3 cut(s) 23, 33, 250
EagI CGGCCG 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 204, 417
EarI CTCTTC 2 cut(s) 204, 417
Ecl136II GAGCTC 3 cut(s) 106, 265, 289
EclXI CGGCCG 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 323
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 3 cut(s) 108, 267, 291
Eco47I GGWCC 4 cut(s) 76, 94, 130, 407
Eco52I CGGCCG 1 cut(s) 23
Eco53kI GAGCTC 3 cut(s) 106, 265, 289
Eco81I CCTNAGG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 3 cut(s) 106, 265, 289
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 4 cut(s) 35, 132, 252, 403
EcoT14I CCWWGG 1 cut(s) 323
EcoT38I GRGCYC 3 cut(s) 108, 267, 291
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 1 cut(s) 327
FaiI YATR 1 cut(s) 325
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 323
Fnu4HI GCNGC 3 cut(s) 205, 247, 250
FokI GGATG 2 cut(s) 125, 482
FriOI GRGCYC 3 cut(s) 108, 267, 291
Fsp4HI GCNGC 3 cut(s) 205, 247, 250
GlaI GCGC 3 cut(s) 185, 279, 281
GluI GCNGC 3 cut(s) 205, 247, 250
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 7 cut(s) 25, 35, 137, 167, 227, 252, 444
HapII CCGG 1 cut(s) 300
HhaI GCGC 3 cut(s) 186, 280, 282
Hin1II CATG 1 cut(s) 327
Hin6I GCGC 3 cut(s) 184, 278, 280
HinP1I GCGC 3 cut(s) 184, 278, 280
HpaII CCGG 1 cut(s) 300
HphI GGTGA 4 cut(s) 52, 289, 367, 475
Hpy188I TCNGA 2 cut(s) 20, 499
Hpy188III TCNNGA 5 cut(s) 172, 232, 335, 361, 416
Hpy99I CGWCG 2 cut(s) 173, 287
HpyAV CCTTC 2 cut(s) 146, 434
HpyCH4IV ACGT 2 cut(s) 72, 387
HpyCH4V TGCA 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 286
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 2 cut(s) 72, 387
Hsp92II CATG 1 cut(s) 327
HspAI GCGC 3 cut(s) 184, 278, 280
Kzo9I GATC 4 cut(s) 15, 309, 319, 357
LmnI GCTCC 2 cut(s) 262, 376
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 1 cut(s) 358
MabI ACCWGGT 1 cut(s) 403
MaeII ACGT 2 cut(s) 72, 387
MaeIII GTNAC 1 cut(s) 481
MalI GATC 4 cut(s) 17, 311, 321, 359
MboI GATC 4 cut(s) 15, 309, 319, 357
MboII GAAGA 3 cut(s) 221, 247, 404
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 3 cut(s) 108, 267, 291
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 4 cut(s) 37, 134, 254, 405
MvaI CCWGG 4 cut(s) 37, 134, 254, 405
MvnI CGCG 4 cut(s) 184, 207, 280, 282
MwoI GCNNNNNNNGC 2 cut(s) 183, 286
NcoI CCATGG 1 cut(s) 323
NdeII GATC 4 cut(s) 15, 309, 319, 357
NlaIII CATG 1 cut(s) 327
NlaIV GGNNCC 2 cut(s) 78, 321
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 481
PauI GCGCGC 1 cut(s) 278
PceI AGGCCT 1 cut(s) 444
PflFI GACNNNGTC 2 cut(s) 74, 405
PkrI GCNGC 3 cut(s) 206, 248, 251
Ple19I CGATCG 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 94
Psp124BI GAGCTC 3 cut(s) 108, 267, 291
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 4 cut(s) 35, 132, 252, 403
PspGI CCWGG 4 cut(s) 35, 132, 252, 403
PspN4I GGNNCC 2 cut(s) 78, 321
PspPI GGNCC 4 cut(s) 76, 94, 130, 407
PspPPI RGGWCCY 1 cut(s) 94
PsuI RGATCY 1 cut(s) 319
PsyI GACNNNGTC 2 cut(s) 74, 405
PteI GCGCGC 1 cut(s) 278
PvuI CGATCG 1 cut(s) 18
SacI GAGCTC 3 cut(s) 108, 267, 291
SatI GCNGC 3 cut(s) 205, 247, 250
Sau3AI GATC 4 cut(s) 15, 309, 319, 357
Sau96I GGNCC 4 cut(s) 76, 94, 130, 407
ScrFI CCNGG 4 cut(s) 37, 134, 254, 405
SduI GDGCHC 3 cut(s) 108, 267, 291
SexAI ACCWGGT 1 cut(s) 403
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 4 cut(s) 76, 94, 130, 407
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 4 cut(s) 160, 190, 205, 249
SstI GAGCTC 3 cut(s) 108, 267, 291
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 4 cut(s) 35, 132, 252, 403
StyI CCWWGG 1 cut(s) 323
TaiI ACGT 2 cut(s) 75, 390
TaqI TCGA 7 cut(s) 102, 171, 231, 258, 270, 312, 360
TauI GCSGC 2 cut(s) 207, 252
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 1 cut(s) 481
Tth111I GACNNNGTC 2 cut(s) 74, 405
VpaK11BI GGWCC 4 cut(s) 76, 94, 130, 407
XagI CCTNNNNNAGG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.