pycom01g22080

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010215
Physical Location & Seq
Forward (+)
19814741 .. 19816183
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22080.1

Sequence Viewer

Length: 513 bp
ATGTTCCTGCGAGCGATCGGACGGCCGTTGTTGGCCAGGGTGAAGCAGACGACGGGGATTGTAGGGCTGGACGTGGTCCCCAATGCGAGGGAGGTCCTAATCGAGCTCTACTCCAAAACCCTAAAGGAGGTCCAGGCCGTCCCCGAGGACGAAGGCTACCGCAAGGCCGTCGAGAGCTTCACGCGCCACCGCCTCAATGTCTGCCGCGAAGAGGAGGACTGGGAGGCCATCGAGAAGAAGCTCGGCTGCGGCCAGGTCGAGGAGCTCATCGAGGAGGCGCGCGACGAGCTTACTCTCATCGGCAAGATGATCGAGTGGGATCGATCGGGTGTTCCTGATGATTACGAATGTGAGGTGATCGAGAACAATGCTCCAGTTCCCAAACATGTTCCTCTGCACCGACCTGGTCCTCTTCCTGAGGAGTTTTACAAAACGCTGGAAGGACTTACTACAAACCAACCAAAGTTGGATGAGGCTAAAGTCACCTCTATTGGTTCAGAAGCAAAGGAGTAA

Protein Analysis

171

Amino Acids

19.27

Weight (kDa)

4.9

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETC_C1_NDUFA5 PF04716 22 - 88 9.8e-29 ETC complex I subunit conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 184, 207, 280, 282
AciI CCGC 4 cut(s) 160, 190, 205, 249
AclWI GGATC 1 cut(s) 327
AcoI YGGCCR 3 cut(s) 23, 33, 250
AfiI CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
AflIII ACRYGT 1 cut(s) 385
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 4 cut(s) 35, 132, 252, 403
AluBI AGCT 5 cut(s) 106, 177, 241, 265, 289
AluI AGCT 5 cut(s) 106, 177, 241, 265, 289
Alw21I GWGCWC 2 cut(s) 108, 267
AlwI GGATC 1 cut(s) 327
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 6 cut(s) 23, 33, 135, 165, 225, 250
ApeKI GCWGC 1 cut(s) 246
AspLEI GCGC 3 cut(s) 186, 280, 282
AspS9I GGNCC 4 cut(s) 76, 94, 130, 407
AsuHPI GGTGA 3 cut(s) 52, 367, 475
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 4 cut(s) 76, 94, 130, 407
AxyI CCTNAGG 1 cut(s) 417
BalI TGGCCA 1 cut(s) 35
BanII GRGCYC 2 cut(s) 108, 267
Bbv12I GWGCWC 2 cut(s) 108, 267
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 236
BceAI ACGGC 4 cut(s) 10, 38, 122, 152
BcgI CGANNNNNNTGC 6 cut(s) 151, 185, 292, 326, 350, 384
BciT130I CCWGG 4 cut(s) 37, 134, 254, 405
BisI GCNGC 3 cut(s) 205, 247, 250
BlsI GCNGC 3 cut(s) 206, 248, 251
Bme1390I CCNGG 4 cut(s) 37, 134, 254, 405
Bme18I GGWCC 4 cut(s) 76, 94, 130, 407
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 4 cut(s) 76, 94, 130, 407
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 4 cut(s) 37, 134, 254, 405
BmrI ACTGGG 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 1 cut(s) 357
Bsa29I ATCGAT 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 36, 144
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
Bse1I ACTGG 2 cut(s) 224, 374
Bse21I CCTNAGG 1 cut(s) 417
BseBI CCWGG 4 cut(s) 37, 134, 254, 405
BseCI ATCGAT 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 36, 144
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 224, 374
BsePI GCGCGC 1 cut(s) 278
BseRI GAGGAG 4 cut(s) 227, 275, 287, 434
BseX3I CGGCCG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 233
BsgI GTGCAG 1 cut(s) 380
Bsh1236I CGCG 4 cut(s) 184, 207, 280, 282
Bsh1285I CGRYCG 3 cut(s) 18, 26, 326
BshFI GGCC 6 cut(s) 25, 35, 137, 167, 227, 252
BshVI ATCGAT 1 cut(s) 322
BsiEI CGRYCG 3 cut(s) 18, 26, 326
BsiHKAI GWGCWC 2 cut(s) 108, 267
BsiHKCI CYCGRG 1 cut(s) 143
BslFI GGGAC 2 cut(s) 62, 125
BslI CCNNNNNNNGG 4 cut(s) 87, 127, 211, 259
BsmFI GGGAC 2 cut(s) 62, 125
BsnI GGCC 6 cut(s) 25, 35, 137, 167, 227, 252
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 108, 267
Bsp143I GATC 5 cut(s) 15, 309, 319, 323, 357
BspACI CCGC 4 cut(s) 160, 190, 205, 249
BspANI GGCC 6 cut(s) 25, 35, 137, 167, 227, 252
BspCNI CTCAG 1 cut(s) 409
BspDI ATCGAT 1 cut(s) 322
BspFNI CGCG 4 cut(s) 184, 207, 280, 282
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 327
BsrI ACTGG 2 cut(s) 224, 374
BssECI CCNNGG 2 cut(s) 36, 144
BssHII GCGCGC 1 cut(s) 278
BssMI GATC 5 cut(s) 15, 309, 319, 323, 357
Bst2UI CCWGG 4 cut(s) 37, 134, 254, 405
Bst6I CTCTTC 2 cut(s) 204, 417
BstC8I GCNNGC 2 cut(s) 12, 280
BstDEI CTNAG 1 cut(s) 417
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 475
BstFNI CGCG 4 cut(s) 184, 207, 280, 282
BstHHI GCGC 3 cut(s) 186, 280, 282
BstKTI GATC 5 cut(s) 18, 312, 322, 326, 360
BstMBI GATC 5 cut(s) 15, 309, 319, 323, 357
BstMCI CGRYCG 3 cut(s) 18, 26, 326
BstMWI GCNNNNNNNGC 2 cut(s) 183, 286
BstNI CCWGG 4 cut(s) 37, 134, 254, 405
BstNSI RCATGY 1 cut(s) 389
BstSCI CCNGG 4 cut(s) 35, 132, 252, 403
BstUI CGCG 4 cut(s) 184, 207, 280, 282
BstV1I GCAGC 1 cut(s) 233
BstZI CGGCCG 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 322
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 6 cut(s) 25, 35, 137, 167, 227, 252
BsuTUI ATCGAT 1 cut(s) 322
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 12, 280
CfoI GCGC 3 cut(s) 186, 280, 282
Cfr13I GGNCC 4 cut(s) 76, 94, 130, 407
ClaI ATCGAT 1 cut(s) 322
CsiI ACCWGGT 1 cut(s) 403
CviAII CATG 1 cut(s) 386
DdeI CTNAG 1 cut(s) 417
DpnI GATC 5 cut(s) 17, 311, 321, 325, 359
DpnII GATC 5 cut(s) 15, 309, 319, 323, 357
EaeI YGGCCR 3 cut(s) 23, 33, 250
EagI CGGCCG 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 204, 417
EarI CTCTTC 2 cut(s) 204, 417
Ecl136II GAGCTC 2 cut(s) 106, 265
EclXI CGGCCG 1 cut(s) 23
Eco24I GRGCYC 2 cut(s) 108, 267
Eco47I GGWCC 4 cut(s) 76, 94, 130, 407
Eco52I CGGCCG 1 cut(s) 23
Eco53kI GAGCTC 2 cut(s) 106, 265
Eco81I CCTNAGG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 2 cut(s) 106, 265
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 4 cut(s) 35, 132, 252, 403
EcoT38I GRGCYC 2 cut(s) 108, 267
FaeI CATG 1 cut(s) 389
FaiI YATR 1 cut(s) 387
FaqI GGGAC 2 cut(s) 62, 125
FatI CATG 1 cut(s) 385
Fnu4HI GCNGC 3 cut(s) 205, 247, 250
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 2 cut(s) 108, 267
Fsp4HI GCNGC 3 cut(s) 205, 247, 250
GlaI GCGC 3 cut(s) 185, 279, 281
GluI GCNGC 3 cut(s) 205, 247, 250
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 6 cut(s) 25, 35, 137, 167, 227, 252
HhaI GCGC 3 cut(s) 186, 280, 282
Hin1II CATG 1 cut(s) 389
Hin6I GCGC 3 cut(s) 184, 278, 280
HinP1I GCGC 3 cut(s) 184, 278, 280
HphI GGTGA 3 cut(s) 52, 367, 475
Hpy188I TCNGA 2 cut(s) 20, 499
Hpy188III TCNNGA 5 cut(s) 172, 232, 335, 361, 416
Hpy99I CGWCG 3 cut(s) 55, 173, 287
HpyAV CCTTC 2 cut(s) 146, 434
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 286
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 1 cut(s) 389
HspAI GCGC 3 cut(s) 184, 278, 280
Kzo9I GATC 5 cut(s) 15, 309, 319, 323, 357
LmnI GCTCC 2 cut(s) 262, 376
Lsp1109I GCAGC 1 cut(s) 233
MabI ACCWGGT 1 cut(s) 403
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 481
MalI GATC 5 cut(s) 17, 311, 321, 325, 359
MboI GATC 5 cut(s) 15, 309, 319, 323, 357
MboII GAAGA 3 cut(s) 221, 247, 404
MhlI GDGCHC 2 cut(s) 108, 267
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 35
MspR9I CCNGG 4 cut(s) 37, 134, 254, 405
MvaI CCWGG 4 cut(s) 37, 134, 254, 405
MvnI CGCG 4 cut(s) 184, 207, 280, 282
MwoI GCNNNNNNNGC 2 cut(s) 183, 286
NdeII GATC 5 cut(s) 15, 309, 319, 323, 357
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 1 cut(s) 78
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 481
NspI RCATGY 1 cut(s) 389
PauI GCGCGC 1 cut(s) 278
PciI ACATGT 1 cut(s) 385
PflFI GACNNNGTC 2 cut(s) 74, 405
PkrI GCNGC 3 cut(s) 206, 248, 251
Ple19I CGATCG 2 cut(s) 18, 326
PpuMI RGGWCCY 1 cut(s) 94
PscI ACATGT 1 cut(s) 385
Psp124BI GAGCTC 2 cut(s) 108, 267
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 4 cut(s) 35, 132, 252, 403
PspGI CCWGG 4 cut(s) 35, 132, 252, 403
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 4 cut(s) 76, 94, 130, 407
PspPPI RGGWCCY 1 cut(s) 94
PsyI GACNNNGTC 2 cut(s) 74, 405
PteI GCGCGC 1 cut(s) 278
PvuI CGATCG 2 cut(s) 18, 326
SacI GAGCTC 2 cut(s) 108, 267
SatI GCNGC 3 cut(s) 205, 247, 250
Sau3AI GATC 5 cut(s) 15, 309, 319, 323, 357
Sau96I GGNCC 4 cut(s) 76, 94, 130, 407
ScrFI CCNGG 4 cut(s) 37, 134, 254, 405
SduI GDGCHC 2 cut(s) 108, 267
SexAI ACCWGGT 1 cut(s) 403
SinI GGWCC 4 cut(s) 76, 94, 130, 407
SsiI CCGC 4 cut(s) 160, 190, 205, 249
SstI GAGCTC 2 cut(s) 108, 267
StyD4I CCNGG 4 cut(s) 35, 132, 252, 403
TaiI ACGT 1 cut(s) 75
TaqI TCGA 8 cut(s) 102, 171, 231, 258, 270, 312, 322, 360
TauI GCSGC 2 cut(s) 207, 252
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 1 cut(s) 481
Tth111I GACNNNGTC 2 cut(s) 74, 405
VpaK11BI GGWCC 4 cut(s) 76, 94, 130, 407
XagI CCTNNNNNAGG 1 cut(s) 125
XceI RCATGY 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.