Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005199

Physical Location & Seq

Genomic Coordinates Forward (+)

30562234 .. 30567083

Gene Structure 4850 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1212200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGCAGTATGCTGAGGAGCTTGTGAGGGAGTTTCTTGTGTTTAGAGGATTCACCAACACTTTGCAAGCTTTTGATTCCGAATTATCTACGGATATCGGTAAAGGGTTTCAAGTGGATAGGATAATGGAATTAATCTTCTCAGTGTATGTGCCTAAGTTTCAGGCAGATAAATTAGTTGGCCTGTTCAGTTTTTTCAAGCTGTGTCTCTCTTCATCATCGGAGACCGTACTTTTGTCTACTCTTTCTAAATTGGAGGTGTCAATTCTACGGTACTACGTTGTTAATGCCATCCAGTCAGGGAGGAGGGACAAAGTTTTAGAGTTCTTTGGAATGAATGGGAATGATTTGTCGCAAAGGAGCGAGGATTGGACTGCATGGTTTGCCATTCCATATGTAAAGAAACCAAACTCTGATCCTGAGTTTCGTATATACTTTTCAAAGGAATGGTACGAGGCCTTGCGTTTATCTGTGAGGAATTTCTTTAGTGAGATCTTCAATGGGGAAGCAGAGGTGGATCCAGATTGGGTTGTTGCTGGAAACATGGGAAGTAGACCAGAATTTAACATATCTAAGTCTGATCCTAATTTAAGTTCTCAATCAAGTCCTCGCATGAGAGATGGTGGAACTGCTGATGGTATTCAGCTGTTCGAGGATGGCACCTATAATGAAAATGGCAGGGAAAGCCATGTAGAAGACTTCCCTGAAGTTAAGGTTGACTTCCAGGAGACATTTTTGGGCCACACAAGTCCAATAACTCGCTGTCGCTTTTCTGCATCTGGAAACAATATAGCCAGCGCATCTGAAGATGGCACAGTAAGGATATGGACGTATGACTCATCAACTCCGTCATCTAGAAATGCAACAATCTATTGTGGAGCCAAGATATTGTCACTTGACTGGGAGTGTAAATCTGACCGATTGCTTCTCATAGGCACTGCTGATGGAGGCATTAAAGCATGGAATGTCGATGCAAAGAGAGTTGTCTGTGATCTTAGCACGAGTCAAGAATTTCCTAGTATCTTGGATATAAAGTGCAGCCCTGTAGAGCCAATTTTTGTTACTGCAGCAGCATCCAAGGGGCACGGATCAAGTTTTGTTGATAGTATGGGGTTTGCTTCATTAACCGTGTGGAACATGAAGACGTGGAAGGCTATGACAGTTCTTCCTCTTGGTAAAGATCCACCTGCAATTACATCCCTTAGTTTCAATCACAATGGGAAAATTTTAGCGGCCGCTGCAACTGATGGAATGATTCACATGTTTGATATGTCTGCCTGTCTACAAATAACTGGGTGGCCTGCACATGACTCTGCTATAAGCTCCATTCTTTTCGGGCCTGATGAGACAAGTATTTTCAGTTTGGGAGTAGACGGAAAGGTGTTTGAATGGAGCTTGCAAAACCAAGGTCAAATCCTTTGGTCAAGGAACTGTAGCAGGTTCTGTGACCCCGAGAGCTCAAAAAATTGCAGACATGAAATGGCTTTAGATGCCAATGGGAGGAGACTGTTGGTCACATCCGGTTCTGTAAGAGCACCCATATACCAGGTCCGGGGTCATGCGAATGGGTTGAGAACTCTCCCACACAGTGCTGCCGTAACTACTGTAGATTGGCACCCAACTTTACCCATCTTCTTAACAGGATCAGCTGATAACTCCGTCCGCGTAACATCTTTGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005829 GO:0006810 GO:0008150 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016482 GO:0019207 GO:0019216 GO:0019220 GO:0019222 GO:0019898 GO:0030234 GO:0031090 GO:0031312 GO:0031313 GO:0031323 GO:0031410 GO:0031901 GO:0031902 GO:0031982 GO:0035014 GO:0042325 GO:0043226 GO:0043227 GO:0043229 GO:0043549 GO:0043550 GO:0043551 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045022 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051174 GO:0051179 GO:0051234 GO:0051338 GO:0051641 GO:0051649 GO:0065007 GO:0065009 GO:0080090 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098927 GO:1903725

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

560

Amino Acids

61.97

Weight (kDa)

5.36

Isoelectric Point (pI)

36.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CTLH_Armc9	PF23138	51 - 166	4.5e-19	Armc9, CTLH-like domain
WD40_Prp19	PF24814	226 - 423	2e-14	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	229 - 338	1.5e-09	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	238 - 330	2.8e-08	TEP-1 second beta-propeller
Beta-prop_EML	PF23409	241 - 465	7e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	241 - 354	8.6e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	241 - 337	8.5e-09	WDHD1 first WD40 domain
WD40	PF00400	242 - 275	2.5e-09	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	243 - 278	4.6e-06	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	247 - 466	9.1e-20	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	247 - 468	1.9e-07	Aladin seven-bladed propeller
Beta-prop_WDR3_2nd	PF25172	366 - 467	6.3e-07	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	369 - 471	2.9e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	373 - 556	7.2e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	373 - 468	2.1e-10	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	373 - 555	1.2e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	396 - 502	5.7e-11	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005199)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700
malus_domestica	MD01G1212200.v1.1 MD07G1282600.v1.1
prunus_persica	Prupe.2G304700_v2.0.a1 Prupe.2G304700_v2.0.a1 Prupe.2G304700_v2.0.a1 Prupe.2G304700_v2.0.a1
pyrus_communis	pycom01g22250
rosa_chinensis	RchiOBHm_Chr1g0379661
rosa_laevigata	RLG00000026372
rosa_multiflora	Rmu_sc0013028.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279980
rosa_rugosa	Rorug01G0413900 Rorug01G0414000.1 Rorug01G0414100.1 Rorug01G0414200.1
rosa_samantha	Rh1AG437700 Rh1BG393900 Rh1CG406800 Rh1DG424800
rosa_wichuraiana	Rw1G038140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1192
Acc36I	ACCTGC	2 cut(s)	1192, 1425
AccB1I	GGYRCC	2 cut(s)	656, 1611
AccI	GTMKAC	4 cut(s)	236, 550, 1279, 1368
AccII	CGCG	1 cut(s)	1662
AciI	CCGC	3 cut(s)	1229, 1233, 1660
AclWI	GGATC	7 cut(s)	407, 509, 522, 572, 1093, 1172, 1648
AcoI	YGGCCR	1 cut(s)	1230
AcsI	RAATTY	4 cut(s)	475, 557, 1007, 1221
AcuI	CTGAAG	2 cut(s)	723, 822
AdeI	CACNNNGTG	1 cut(s)	1586
AfaI	GTAC	3 cut(s)	228, 272, 449
AfiI	CCNNNNNNNGG	2 cut(s)	1497, 1549
AflIII	ACRYGT	1 cut(s)	1257
AgsI	TTSAA	6 cut(s)	110, 196, 438, 496, 1207, 1385
AhdI	GACNNNNNGTC	1 cut(s)	1508
AjiI	CACGTC	1 cut(s)	1143
AjnI	CCWGG	2 cut(s)	720, 1542
AluBI	AGCT	8 cut(s)	19, 68, 199, 643, 1320, 1392, 1455, 1646
AluI	AGCT	8 cut(s)	19, 68, 199, 643, 1320, 1392, 1455, 1646
Alw21I	GWGCWC	2 cut(s)	1457, 1534
Alw26I	GTCTC	5 cut(s)	209, 215, 719, 1337, 1495
AlwI	GGATC	7 cut(s)	407, 509, 522, 572, 1093, 1172, 1648
AlwNI	CAGNNNCTG	1 cut(s)	1440
Ama87I	CYCGRG	1 cut(s)	1448
AoxI	GGCC	6 cut(s)	178, 453, 736, 1230, 1295, 1334
ApeKI	GCWGC	5 cut(s)	1035, 1064, 1067, 1235, 1589
ApoI	RAATTY	4 cut(s)	475, 557, 1007, 1221
ArsI	GACNNNNNNTTYG	4 cut(s)	214, 246, 1390, 1422
AseI	ATTAAT	1 cut(s)	131
AspLEI	GCGC	1 cut(s)	797
AspS9I	GGNCC	3 cut(s)	736, 1334, 1546
AsuC2I	CCSGG	1 cut(s)	1550
AsuHPI	GGTGA	1 cut(s)	43
AvaI	CYCGRG	1 cut(s)	1448
AvaII	GGWCC	1 cut(s)	1546
BaeGI	GKGCMC	1 cut(s)	1083
BamHI	GGATCC	1 cut(s)	514
BanI	GGYRCC	2 cut(s)	656, 1611
BanII	GRGCYC	1 cut(s)	1457
BauI	CACGAG	1 cut(s)	997
BbsI	GAAGAC	2 cut(s)	699, 1145
Bbv12I	GWGCWC	2 cut(s)	1457, 1534
BbvCI	CCTCAGC	1 cut(s)	12
BbvI	GCAGC	5 cut(s)	1047, 1076, 1079, 1222, 1576
BccI	CCATC	8 cut(s)	296, 611, 626, 647, 800, 935, 1238, 1634
BceAI	ACGGC	1 cut(s)	1577
BciT130I	CCWGG	2 cut(s)	722, 1544
BcnI	CCSGG	1 cut(s)	1550
BcoDI	GTCTC	5 cut(s)	209, 215, 719, 1337, 1495
BfaI	CTAG	2 cut(s)	852, 1014
BfmI	CTRYAG	4 cut(s)	1041, 1062, 1429, 1602
BfuAI	ACCTGC	2 cut(s)	1192, 1425
BglII	AGATCT	1 cut(s)	489
BisI	GCNGC	7 cut(s)	1036, 1065, 1068, 1230, 1233, 1236, 1590
BlsI	GCNGC	7 cut(s)	1037, 1066, 1069, 1231, 1234, 1237, 1591
Bme1390I	CCNGG	3 cut(s)	722, 1544, 1550
Bme18I	GGWCC	1 cut(s)	1546
BmeRI	GACNNNNNGTC	1 cut(s)	1508
BmeT110I	CYCGRG	1 cut(s)	1448
BmgBI	CACGTC	1 cut(s)	1143
BmgT120I	GGNCC	3 cut(s)	736, 1334, 1546
BmiI	GGNNCC	4 cut(s)	516, 658, 877, 1613
BmrFI	CCNGG	3 cut(s)	722, 1544, 1550
BmrI	ACTGGG	2 cut(s)	907, 1299
BmsI	GCATC	5 cut(s)	782, 806, 958, 1079, 1477
BmuI	ACTGGG	2 cut(s)	907, 1299
BpiI	GAAGAC	2 cut(s)	699, 1145
Bpu10I	CCTNAGC	1 cut(s)	12
BpuMI	CCSGG	1 cut(s)	1550
BsaI	GGTCTC	1 cut(s)	215
BsaJI	CCNNGG	3 cut(s)	1074, 1402, 1549
BsaWI	WCCGGW	1 cut(s)	1517
Bsc4I	CCNNNNNNNGG	2 cut(s)	1497, 1549
Bse1I	ACTGG	3 cut(s)	292, 902, 1294
BseBI	CCWGG	2 cut(s)	722, 1544
BseDI	CCNNGG	3 cut(s)	1074, 1402, 1549
BseGI	GGATG	5 cut(s)	288, 658, 1070, 1193, 1514
BseLI	CCNNNNNNNGG	2 cut(s)	1497, 1549
BseMII	CTCAG	2 cut(s)	153, 408
BseNI	ACTGG	3 cut(s)	292, 902, 1294
BseRI	GAGGAG	3 cut(s)	29, 316, 1513
BseSI	GKGCMC	1 cut(s)	1083
BseX3I	CGGCCG	1 cut(s)	1230
BseXI	GCAGC	5 cut(s)	1047, 1076, 1079, 1222, 1576
BsgI	GTGCAG	2 cut(s)	1054, 1284
Bsh1236I	CGCG	1 cut(s)	1662
Bsh1285I	CGRYCG	1 cut(s)	1233
BshFI	GGCC	6 cut(s)	180, 455, 738, 1232, 1297, 1336
BshNI	GGYRCC	2 cut(s)	656, 1611
BsiEI	CGRYCG	1 cut(s)	1233
BsiHKAI	GWGCWC	2 cut(s)	1457, 1534
BsiHKCI	CYCGRG	1 cut(s)	1448
BsiSI	CCGG	2 cut(s)	1518, 1549
BslFI	GGGAC	1 cut(s)	320
BslI	CCNNNNNNNGG	2 cut(s)	1497, 1549
BsmAI	GTCTC	5 cut(s)	209, 215, 719, 1337, 1495
BsmFI	GGGAC	1 cut(s)	320
BsnI	GGCC	6 cut(s)	180, 455, 738, 1232, 1297, 1336
Bso31I	GGTCTC	1 cut(s)	215
BsoBI	CYCGRG	1 cut(s)	1448
Bsp1286I	GDGCHC	3 cut(s)	1083, 1457, 1534
Bsp143I	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
BspACI	CCGC	3 cut(s)	1229, 1233, 1660
BspANI	GGCC	6 cut(s)	180, 455, 738, 1232, 1297, 1336
BspCNI	CTCAG	3 cut(s)	4, 152, 409
BspFNI	CGCG	1 cut(s)	1662
BspLI	GGNNCC	4 cut(s)	516, 658, 877, 1613
BspMAI	CTGCAG	1 cut(s)	1066
BspMI	ACCTGC	2 cut(s)	1192, 1425
BspPI	GGATC	7 cut(s)	407, 509, 522, 572, 1093, 1172, 1648
BspT107I	GGYRCC	2 cut(s)	656, 1611
BspTNI	GGTCTC	1 cut(s)	215
BsrI	ACTGG	3 cut(s)	292, 902, 1294
BssECI	CCNNGG	3 cut(s)	1074, 1402, 1549
BssMI	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
BssSI	CACGAG	1 cut(s)	997
BssT1I	CCWWGG	2 cut(s)	1074, 1402
Bst2BI	CACGAG	1 cut(s)	997
Bst2UI	CCWGG	2 cut(s)	722, 1544
Bst4CI	ACNGT	9 cut(s)	226, 270, 814, 1126, 1159, 1430, 1506, 1586, 1603
Bst6I	CTCTTC	1 cut(s)	214
BstAPI	GCANNNNNTGC	1 cut(s)	380
BstC8I	GCNNGC	4 cut(s)	66, 793, 1299, 1394
BstDEI	CTNAG	7 cut(s)	12, 139, 153, 417, 570, 992, 1199
BstF5I	GGATG	5 cut(s)	288, 658, 1070, 1193, 1514
BstFNI	CGCG	1 cut(s)	1662
BstHHI	GCGC	1 cut(s)	797
BstKTI	GATC	8 cut(s)	415, 492, 517, 580, 991, 1088, 1180, 1643
BstMAI	GTCTC	5 cut(s)	209, 215, 719, 1337, 1495
BstMBI	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
BstMCI	CGRYCG	1 cut(s)	1233
BstMWI	GCNNNNNNNGC	4 cut(s)	380, 681, 1235, 1487
BstNI	CCWGG	2 cut(s)	722, 1544
BstNSI	RCATGY	1 cut(s)	1261
BstSCI	CCNGG	3 cut(s)	720, 1542, 1548
BstSFI	CTRYAG	4 cut(s)	1041, 1062, 1429, 1602
BstSLI	GKGCMC	1 cut(s)	1083
BstUI	CGCG	1 cut(s)	1662
BstV1I	GCAGC	5 cut(s)	1047, 1076, 1079, 1222, 1576
BstV2I	GAAGAC	2 cut(s)	699, 1145
BstX2I	RGATCY	3 cut(s)	489, 514, 1177
BstYI	RGATCY	3 cut(s)	489, 514, 1177
BstZI	CGGCCG	1 cut(s)	1230
BsuRI	GGCC	6 cut(s)	180, 455, 738, 1232, 1297, 1336
BtrI	CACGTC	1 cut(s)	1143
BtsCI	GGATG	5 cut(s)	288, 658, 1070, 1193, 1514
BtsI	GCAGTG	1 cut(s)	933
BtsIMutI	CAGTG	3 cut(s)	147, 933, 1591
BveI	ACCTGC	2 cut(s)	1192, 1425
Cac8I	GCNNGC	4 cut(s)	66, 793, 1299, 1394
CaiI	CAGNNNCTG	1 cut(s)	1440
CciNI	GCGGCCGC	1 cut(s)	1230
CfoI	GCGC	1 cut(s)	797
Cfr13I	GGNCC	3 cut(s)	736, 1334, 1546
CsiI	ACCWGGT	1 cut(s)	1542
Csp6I	GTAC	3 cut(s)	227, 271, 448
CviQI	GTAC	3 cut(s)	227, 271, 448
DdeI	CTNAG	7 cut(s)	12, 139, 153, 417, 570, 992, 1199
DpnI	GATC	8 cut(s)	414, 491, 516, 579, 990, 1087, 1179, 1642
DpnII	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
DraIII	CACNNNGTG	1 cut(s)	1586
DriI	GACNNNNNGTC	1 cut(s)	1508
EaeI	YGGCCR	1 cut(s)	1230
EagI	CGGCCG	1 cut(s)	1230
Eam1104I	CTCTTC	1 cut(s)	214
Eam1105I	GACNNNNNGTC	1 cut(s)	1508
EarI	CTCTTC	1 cut(s)	214
Ecl136II	GAGCTC	1 cut(s)	1455
EclXI	CGGCCG	1 cut(s)	1230
Eco130I	CCWWGG	2 cut(s)	1074, 1402
Eco147I	AGGCCT	1 cut(s)	455
Eco24I	GRGCYC	1 cut(s)	1457
Eco31I	GGTCTC	1 cut(s)	215
Eco32I	GATATC	1 cut(s)	94
Eco47I	GGWCC	1 cut(s)	1546
Eco52I	CGGCCG	1 cut(s)	1230
Eco53kI	GAGCTC	1 cut(s)	1455
Eco57I	CTGAAG	2 cut(s)	723, 822
Eco88I	CYCGRG	1 cut(s)	1448
EcoICRI	GAGCTC	1 cut(s)	1455
EcoRII	CCWGG	2 cut(s)	720, 1542
EcoRV	GATATC	1 cut(s)	94
EcoT14I	CCWWGG	2 cut(s)	1074, 1402
EcoT38I	GRGCYC	1 cut(s)	1457
ErhI	CCWWGG	2 cut(s)	1074, 1402
FalI	AAGNNNNNCTT	2 cut(s)	701, 733
FaqI	GGGAC	1 cut(s)	320
FauNDI	CATATG	1 cut(s)	391
FblI	GTMKAC	4 cut(s)	236, 550, 1279, 1368
Fnu4HI	GCNGC	7 cut(s)	1036, 1065, 1068, 1230, 1233, 1236, 1590
FokI	GGATG	5 cut(s)	275, 665, 1057, 1180, 1501
FriOI	GRGCYC	1 cut(s)	1457
Fsp4HI	GCNGC	7 cut(s)	1036, 1065, 1068, 1230, 1233, 1236, 1590
FspBI	CTAG	2 cut(s)	852, 1014
GlaI	GCGC	1 cut(s)	796
GluI	GCNGC	7 cut(s)	1036, 1065, 1068, 1230, 1233, 1236, 1590
HaeIII	GGCC	6 cut(s)	180, 455, 738, 1232, 1297, 1336
HapII	CCGG	2 cut(s)	1518, 1549
HhaI	GCGC	1 cut(s)	797
Hin6I	GCGC	1 cut(s)	795
HinP1I	GCGC	1 cut(s)	795
HincII	GTYRAC	1 cut(s)	715
HindII	GTYRAC	1 cut(s)	715
HindIII	AAGCTT	1 cut(s)	66
HinfI	GANTC	6 cut(s)	48, 74, 833, 1000, 1252, 1307
HpaII	CCGG	2 cut(s)	1518, 1549
HphI	GGTGA	1 cut(s)	43
Hpy166II	GTNNAC	5 cut(s)	237, 551, 715, 1280, 1369
Hpy188I	TCNGA	6 cut(s)	79, 220, 412, 577, 802, 913
Hpy188III	TCNNGA	5 cut(s)	416, 518, 777, 852, 1004
Hpy8I	GTNNAC	5 cut(s)	237, 551, 715, 1280, 1369
HpyAV	CCTTC	1 cut(s)	1141
HpyCH4III	ACNGT	9 cut(s)	226, 270, 814, 1126, 1159, 1430, 1506, 1586, 1603
HpyCH4IV	ACGT	3 cut(s)	276, 827, 1142
HpyF10VI	GCNNNNNNNGC	4 cut(s)	380, 681, 1235, 1487
HpyF3I	CTNAG	7 cut(s)	12, 139, 153, 417, 570, 992, 1199
HpySE526I	ACGT	3 cut(s)	276, 827, 1142
HspAI	GCGC	1 cut(s)	795
Kzo9I	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
LmnI	GCTCC	5 cut(s)	16, 357, 875, 1325, 1389
Lsp1109I	GCAGC	5 cut(s)	1047, 1076, 1079, 1222, 1576
LweI	GCATC	5 cut(s)	782, 806, 958, 1079, 1477
MabI	ACCWGGT	1 cut(s)	1542
MaeI	CTAG	2 cut(s)	852, 1014
MaeII	ACGT	3 cut(s)	276, 827, 1142
MaeIII	GTNAC	6 cut(s)	888, 1057, 1442, 1510, 1594, 1663
MalI	GATC	8 cut(s)	414, 491, 516, 579, 990, 1087, 1179, 1642
MboI	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
MboII	GAAGA	8 cut(s)	127, 201, 484, 704, 815, 1150, 1154, 1621
MflI	RGATCY	3 cut(s)	489, 514, 1177
MhlI	GDGCHC	3 cut(s)	1083, 1457, 1534
MlyI	GAGTC	3 cut(s)	827, 1009, 1301
MseI	TTAA	8 cut(s)	131, 282, 561, 587, 708, 951, 1121, 1634
MslI	CAYNNNNRTG	1 cut(s)	1560
MspA1I	CMGCKG	3 cut(s)	643, 1235, 1646
MspI	CCGG	2 cut(s)	1518, 1549
MspR9I	CCNGG	3 cut(s)	722, 1544, 1550
MvaI	CCWGG	2 cut(s)	722, 1544
MvnI	CGCG	1 cut(s)	1662
MwoI	GCNNNNNNNGC	4 cut(s)	380, 681, 1235, 1487
NciI	CCSGG	1 cut(s)	1550
NdeI	CATATG	1 cut(s)	391
NdeII	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
NlaIV	GGNNCC	4 cut(s)	516, 658, 877, 1613
NmuCI	GTSAC	3 cut(s)	888, 1442, 1510
NotI	GCGGCCGC	1 cut(s)	1230
NspI	RCATGY	1 cut(s)	1261
PaqCI	CACCTGC	1 cut(s)	1192
PceI	AGGCCT	1 cut(s)	455
PciI	ACATGT	1 cut(s)	1257
PfeI	GAWTC	3 cut(s)	48, 74, 1252
PfoI	TCCNGGA	1 cut(s)	720
PkrI	GCNGC	7 cut(s)	1037, 1066, 1069, 1231, 1234, 1237, 1591
PleI	GAGTC	3 cut(s)	827, 1008, 1301
PpsI	GAGTC	3 cut(s)	827, 1008, 1301
PscI	ACATGT	1 cut(s)	1257
PshBI	ATTAAT	1 cut(s)	131
Psp124BI	GAGCTC	1 cut(s)	1457
Psp6I	CCWGG	2 cut(s)	720, 1542
PspGI	CCWGG	2 cut(s)	720, 1542
PspN4I	GGNNCC	4 cut(s)	516, 658, 877, 1613
PspPI	GGNCC	3 cut(s)	736, 1334, 1546
PstI	CTGCAG	1 cut(s)	1066
PstNI	CAGNNNCTG	1 cut(s)	1440
PsuI	RGATCY	3 cut(s)	489, 514, 1177
PvuII	CAGCTG	2 cut(s)	643, 1646
RsaI	GTAC	3 cut(s)	228, 272, 449
RsaNI	GTAC	3 cut(s)	227, 271, 448
RseI	CAYNNNNRTG	1 cut(s)	1560
SacI	GAGCTC	1 cut(s)	1457
SaqAI	TTAA	8 cut(s)	131, 282, 561, 587, 708, 951, 1121, 1634
SatI	GCNGC	7 cut(s)	1036, 1065, 1068, 1230, 1233, 1236, 1590
Sau3AI	GATC	8 cut(s)	412, 489, 514, 577, 988, 1085, 1177, 1640
Sau96I	GGNCC	3 cut(s)	736, 1334, 1546
SchI	GAGTC	3 cut(s)	827, 1009, 1301
ScrFI	CCNGG	3 cut(s)	722, 1544, 1550
SduI	GDGCHC	3 cut(s)	1083, 1457, 1534
SexAI	ACCWGGT	1 cut(s)	1542
SfaNI	GCATC	5 cut(s)	782, 806, 958, 1079, 1477
SfcI	CTRYAG	4 cut(s)	1041, 1062, 1429, 1602
SinI	GGWCC	1 cut(s)	1546
SmiMI	CAYNNNNRTG	1 cut(s)	1560
SseBI	AGGCCT	1 cut(s)	455
SsiI	CCGC	3 cut(s)	1229, 1233, 1660
SspMI	CTAG	2 cut(s)	852, 1014
SstI	GAGCTC	1 cut(s)	1457
StuI	AGGCCT	1 cut(s)	455
StyD4I	CCNGG	3 cut(s)	720, 1542, 1548
StyI	CCWWGG	2 cut(s)	1074, 1402
TaaI	ACNGT	9 cut(s)	226, 270, 814, 1126, 1159, 1430, 1506, 1586, 1603
TaiI	ACGT	3 cut(s)	279, 830, 1145
TaqI	TCGA	2 cut(s)	648, 966
TaqII	GACCGA	1 cut(s)	930
TauI	GCSGC	2 cut(s)	1232, 1235
TfiI	GAWTC	3 cut(s)	48, 74, 1252
Tru1I	TTAA	8 cut(s)	131, 282, 561, 587, 708, 951, 1121, 1634
Tru9I	TTAA	8 cut(s)	131, 282, 561, 587, 708, 951, 1121, 1634
TscAI	CASTG	3 cut(s)	147, 940, 1591
TseFI	GTSAC	3 cut(s)	888, 1442, 1510
TseI	GCWGC	5 cut(s)	1035, 1064, 1067, 1235, 1589
Tsp45I	GTSAC	3 cut(s)	888, 1442, 1510
TspDTI	ATGAA	6 cut(s)	201, 347, 681, 1107, 1151, 1488
TspGWI	ACGGA	5 cut(s)	104, 834, 1098, 1386, 1645
TspRI	CASTG	3 cut(s)	147, 940, 1591
VpaK11BI	GGWCC	1 cut(s)	1546
VspI	ATTAAT	1 cut(s)	131
XapI	RAATTY	4 cut(s)	475, 557, 1007, 1221
XbaI	TCTAGA	1 cut(s)	851
XceI	RCATGY	1 cut(s)	1261
XmiI	GTMKAC	4 cut(s)	236, 550, 1279, 1368
XspI	CTAG	2 cut(s)	852, 1014

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.