Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0005199

Physical Location & Seq

Genomic Coordinates Forward (+)

19931783 .. 19935806

Gene Structure 4024 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g22250.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGCAGTATGCTGAGGAGCTTGTGAGGGAGTTTCTTGTGTTTAGAGGATTCACCAACACTTTGCAAACTTTTGATTCCGAATTATCTACGGATATCGGTAAAGGGTTTCAAGTGGATAGGATAATGGAATTAATCTTCTCAGTGTATGTACCTAAGTTTCAGGCAGATAAATTAGTTGGCCTGTTCAGTTTTTTCAAGCTGTGTCTCTCTTCATCATCCGAGACCGTACTTTTGGCTACTCTTTCTAAATTGGAGGTGTCAATTCTACGGTACTACGTTGTTAATGCCATCCAGTCAGGGAGGAGGGACAAAGTTTTAGAGTTCTTTGGAATGAATGGGAATGATTTGTTGCAAAGGGACAAGGATTGGACTGCATGGTTTGCCATTCCATATGTAAAGAAACCAAACTCTGATCCTGAGTTTCGTATTTACTTTTCAAAGGAATGGTACGAGGCCTTGCGTTTATCTGTGAGGAATTTCTTTAGTGAGATCTTCAATGCACGCATGCCAGCCCTTTTGAAAATCAGTTCAGAGAAGACTACTGTCAACCGTCTGAAAAAAGACATCAAGCAACTCAATCTCAAGCTGTCAGAACTCCAGGCTTTGTTGGAGGAAAAAGATGGTGAATTATGCCAGTTAAGAAGTAATTGTTCATCAGTAGCCGATGCAAGCACTGAACAAACCAGGAGTTCATCAAGTGTAGTGCATGAGCAAAATCGTCTTATATCTAAAGATACTCAGGAAACTTGCCCTCCTGATACTTTTCAAATAGAAGAAGCAGAGGTGGATCGATATTGGGTTGTTGCTGGAAACATGGGAAGTAGACCAGAATTTAACATATCTAAGTCTGATCCTTATTTAAGTTCTCAGTCAAGTCCTCGCGTGAGAGATGGTGGAACTGCTGATAGTATTCAGCTGTTCGAGGATGGCACCTATAATGAAAATGGCAGGGAAAGCCATGTAGAAGACTTCCCTGAAGTTAAGGTTGACTTCCAGGAGACATTTTTGGGCCACACAAGTCCAATAACTCGCTGTCGTTTTTCTGCATCTGGAAACAATATAGCCAGCGCCTCTGAAGATGGCACAGTAAGGATATGGACGTATGACTCATCAACTCCGTCATCTAGAAATGCAACCATTTATTGTGGAGCCAAGATCATGTCACTTGACTGGGAGTGTAAATCTGACCGATTGCTTCTCATAGGCACTGCTGATGGAGGCATTAAAGCATGGAATGTCGATGCAAAAAGAGTTGTCTGTGATCTTAGCACGAGTGAAGAATTTCCTAGTATCTTGGATATAAAGTGCAGCCCTGTAGAGCCAATTTTTGTTACTGCAGCAGCATCCAAGGGGTACGGATCAAGTTACGCTGATAATATGGGGTTCGCTTCATTAACCGTGTGGAACATGAAGACGTGGAAGGCCATGACAGTTCTTCCTCTTGGTAAAGATCCACCTGCAATAACATCCCTCAGTTTCAATCACAATGGGAAAATTTTAGCGGCCGCTGCAACTGATGGAATGATTCACATGTTTGATATGTCTGCCTGTCTACAAATAACCGGTTGGCCTGCACATGACTCTGCTATAAGCTCCATTCTTTTCGGGCCTGATGAGACAAGTATTTTCAGTTTGGGAGTAGATGGAAAGGTGTTTGAATGGAGCTTGCAAAACCAAGGTCAAATCCTTTGGTCAAGGAACTGTAGCAGGTTCTGTGACCCCGAGAGCTCAAAAAATTGCAGACATGAAATGGCTTTAGATGCCAATGGGAGGAGACTGTTGGTCACATCCGGTTCTGTAAGAGCACCCATATACCAGGTCCGGGGTCATGCGAATGGGTTGAGAACTCTCCCACACAGTGCTGCCATAACTACTGTAGATTGGCACCCGACGTTACCCATCTTCTTAACAGGATCAGCTGATAACTCTGTCCGAGTGACATCTTTGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005829 GO:0006810 GO:0008150 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016482 GO:0019207 GO:0019216 GO:0019220 GO:0019222 GO:0019898 GO:0030234 GO:0031090 GO:0031312 GO:0031313 GO:0031323 GO:0031410 GO:0031901 GO:0031902 GO:0031982 GO:0035014 GO:0042325 GO:0043226 GO:0043227 GO:0043229 GO:0043549 GO:0043550 GO:0043551 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045022 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051174 GO:0051179 GO:0051234 GO:0051338 GO:0051641 GO:0051649 GO:0065007 GO:0065009 GO:0080090 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098927 GO:1903725

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

651

Amino Acids

72.49

Weight (kDa)

5.5

Isoelectric Point (pI)

40.64

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005199)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700 FvH4_7g30700
malus_domestica	MD01G1212200.v1.1 MD07G1282600.v1.1
prunus_persica	Prupe.2G304700_v2.0.a1 Prupe.2G304700_v2.0.a1 Prupe.2G304700_v2.0.a1 Prupe.2G304700_v2.0.a1
pyrus_communis	pycom01g22250
rosa_chinensis	RchiOBHm_Chr1g0379661
rosa_laevigata	RLG00000026372
rosa_multiflora	Rmu_sc0013028.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279980
rosa_rugosa	Rorug01G0413900 Rorug01G0414000.1 Rorug01G0414100.1 Rorug01G0414200.1
rosa_samantha	Rh1AG437700 Rh1BG393900 Rh1CG406800 Rh1DG424800
rosa_wichuraiana	Rw1G038140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1465
AasI	GACNNNNNNGTC	1 cut(s)	1945
Acc36I	ACCTGC	2 cut(s)	1465, 1698
AccB1I	GGYRCC	2 cut(s)	929, 1884
AccI	GTMKAC	2 cut(s)	823, 1552
AccII	CGCG	1 cut(s)	882
AciI	CCGC	2 cut(s)	1502, 1506
AclWI	GGATC	6 cut(s)	407, 795, 845, 1366, 1445, 1921
AcoI	YGGCCR	1 cut(s)	1503
AcsI	RAATTY	4 cut(s)	475, 830, 1280, 1494
AcuI	CTGAAG	2 cut(s)	996, 1095
AdeI	CACNNNGTG	1 cut(s)	1859
AfaI	GTAC	5 cut(s)	150, 228, 272, 449, 1355
AfiI	CCNNNNNNNGG	2 cut(s)	1770, 1822
AflIII	ACRYGT	1 cut(s)	1530
AgeI	ACCGGT	1 cut(s)	1562
AgsI	TTSAA	8 cut(s)	110, 196, 438, 496, 520, 767, 1480, 1658
AhdI	GACNNNNNGTC	1 cut(s)	1781
AjiI	CACGTC	1 cut(s)	1416
AjnI	CCWGG	4 cut(s)	597, 683, 993, 1815
AluBI	AGCT	8 cut(s)	19, 199, 586, 916, 1593, 1665, 1728, 1919
AluI	AGCT	8 cut(s)	19, 199, 586, 916, 1593, 1665, 1728, 1919
Alw21I	GWGCWC	2 cut(s)	1730, 1807
Alw26I	GTCTC	5 cut(s)	209, 215, 992, 1610, 1768
AlwI	GGATC	6 cut(s)	407, 795, 845, 1366, 1445, 1921
AlwNI	CAGNNNCTG	1 cut(s)	1713
Ama87I	CYCGRG	1 cut(s)	1721
AoxI	GGCC	7 cut(s)	178, 453, 1009, 1422, 1503, 1568, 1607
ApeKI	GCWGC	5 cut(s)	1308, 1337, 1340, 1508, 1862
ApoI	RAATTY	4 cut(s)	475, 830, 1280, 1494
ArsI	GACNNNNNNTTYG	4 cut(s)	214, 246, 1663, 1695
AseI	ATTAAT	1 cut(s)	131
AsiGI	ACCGGT	1 cut(s)	1562
Asp700I	GAANNNNTTC	1 cut(s)	1281
AspLEI	GCGC	1 cut(s)	1070
AspS9I	GGNCC	3 cut(s)	1009, 1607, 1819
AsuC2I	CCSGG	1 cut(s)	1823
AsuHPI	GGTGA	2 cut(s)	43, 635
AvaI	CYCGRG	1 cut(s)	1721
AvaII	GGWCC	1 cut(s)	1819
BanI	GGYRCC	2 cut(s)	929, 1884
BanII	GRGCYC	1 cut(s)	1730
BauI	CACGAG	1 cut(s)	1270
BbsI	GAAGAC	3 cut(s)	542, 972, 1418
Bbv12I	GWGCWC	2 cut(s)	1730, 1807
BbvCI	CCTCAGC	1 cut(s)	12
BbvI	GCAGC	5 cut(s)	1320, 1349, 1352, 1495, 1849
BccI	CCATC	9 cut(s)	296, 614, 884, 920, 1073, 1208, 1511, 1637, 1907
BciT130I	CCWGG	4 cut(s)	599, 685, 995, 1817
BcnI	CCSGG	1 cut(s)	1823
BcoDI	GTCTC	5 cut(s)	209, 215, 992, 1610, 1768
BfaI	CTAG	2 cut(s)	1125, 1287
BfmI	CTRYAG	4 cut(s)	1314, 1335, 1702, 1875
BfoI	RGCGCY	1 cut(s)	1071
BfuAI	ACCTGC	2 cut(s)	1465, 1698
BglII	AGATCT	1 cut(s)	489
BisI	GCNGC	7 cut(s)	1309, 1338, 1341, 1503, 1506, 1509, 1863
BlsI	GCNGC	7 cut(s)	1310, 1339, 1342, 1504, 1507, 1510, 1864
Bme1390I	CCNGG	5 cut(s)	599, 685, 995, 1817, 1823
Bme18I	GGWCC	1 cut(s)	1819
BmeRI	GACNNNNNGTC	1 cut(s)	1781
BmeT110I	CYCGRG	1 cut(s)	1721
BmgBI	CACGTC	1 cut(s)	1416
BmgT120I	GGNCC	3 cut(s)	1009, 1607, 1819
BmiI	GGNNCC	3 cut(s)	931, 1150, 1886
BmrFI	CCNGG	5 cut(s)	599, 685, 995, 1817, 1823
BmrI	ACTGGG	1 cut(s)	1180
BmsI	GCATC	5 cut(s)	655, 1055, 1231, 1352, 1750
BmuI	ACTGGG	1 cut(s)	1180
BoxI	GACNNNNGTC	1 cut(s)	542
BpiI	GAAGAC	3 cut(s)	542, 972, 1418
BpmI	CTGGAG	1 cut(s)	581
Bpu10I	CCTNAGC	1 cut(s)	12
BpuEI	CTTGAG	1 cut(s)	566
BpuMI	CCSGG	1 cut(s)	1823
Bsa29I	ATCGAT	1 cut(s)	790
BsaI	GGTCTC	1 cut(s)	215
BsaJI	CCNNGG	3 cut(s)	1347, 1675, 1822
BsaWI	WCCGGW	2 cut(s)	1562, 1790
BsaXI	ACNNNNNCTCC	2 cut(s)	736, 766
Bsc4I	CCNNNNNNNGG	2 cut(s)	1770, 1822
Bse118I	RCCGGY	1 cut(s)	1562
Bse1I	ACTGG	3 cut(s)	292, 634, 1175
BseBI	CCWGG	4 cut(s)	599, 685, 995, 1817
BseCI	ATCGAT	1 cut(s)	790
BseDI	CCNNGG	3 cut(s)	1347, 1675, 1822
BseGI	GGATG	6 cut(s)	215, 288, 931, 1343, 1466, 1787
BseLI	CCNNNNNNNGG	2 cut(s)	1770, 1822
BseMII	CTCAG	5 cut(s)	153, 408, 752, 881, 1486
BseNI	ACTGG	3 cut(s)	292, 634, 1175
BseRI	GAGGAG	3 cut(s)	29, 316, 1786
BseX3I	CGGCCG	1 cut(s)	1503
BseXI	GCAGC	5 cut(s)	1320, 1349, 1352, 1495, 1849
BsgI	GTGCAG	2 cut(s)	1327, 1557
Bsh1236I	CGCG	1 cut(s)	882
Bsh1285I	CGRYCG	1 cut(s)	1506
BshFI	GGCC	7 cut(s)	180, 455, 1011, 1424, 1505, 1570, 1609
BshNI	GGYRCC	2 cut(s)	929, 1884
BshTI	ACCGGT	1 cut(s)	1562
BshVI	ATCGAT	1 cut(s)	790
BsiEI	CGRYCG	1 cut(s)	1506
BsiHKAI	GWGCWC	2 cut(s)	1730, 1807
BsiHKCI	CYCGRG	1 cut(s)	1721
BsiSI	CCGG	3 cut(s)	1563, 1791, 1822
BslFI	GGGAC	2 cut(s)	320, 371
BslI	CCNNNNNNNGG	2 cut(s)	1770, 1822
BsmAI	GTCTC	5 cut(s)	209, 215, 992, 1610, 1768
BsmFI	GGGAC	2 cut(s)	320, 371
BsnI	GGCC	7 cut(s)	180, 455, 1011, 1424, 1505, 1570, 1609
Bso31I	GGTCTC	1 cut(s)	215
BsoBI	CYCGRG	1 cut(s)	1721
Bsp1286I	GDGCHC	2 cut(s)	1730, 1807
Bsp143I	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
BspACI	CCGC	2 cut(s)	1502, 1506
BspANI	GGCC	7 cut(s)	180, 455, 1011, 1424, 1505, 1570, 1609
BspCNI	CTCAG	6 cut(s)	4, 152, 409, 751, 880, 1485
BspDI	ATCGAT	1 cut(s)	790
BspFNI	CGCG	1 cut(s)	882
BspLI	GGNNCC	3 cut(s)	931, 1150, 1886
BspMAI	CTGCAG	1 cut(s)	1339
BspMI	ACCTGC	2 cut(s)	1465, 1698
BspPI	GGATC	6 cut(s)	407, 795, 845, 1366, 1445, 1921
BspT107I	GGYRCC	2 cut(s)	929, 1884
BspTNI	GGTCTC	1 cut(s)	215
BsrFI	RCCGGY	1 cut(s)	1562
BsrI	ACTGG	3 cut(s)	292, 634, 1175
BssAI	RCCGGY	1 cut(s)	1562
BssECI	CCNNGG	3 cut(s)	1347, 1675, 1822
BssMI	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
BssSI	CACGAG	1 cut(s)	1270
BssT1I	CCWWGG	2 cut(s)	1347, 1675
Bst2BI	CACGAG	1 cut(s)	1270
Bst2UI	CCWGG	4 cut(s)	599, 685, 995, 1817
Bst6I	CTCTTC	1 cut(s)	214
BstAPI	GCANNNNNTGC	1 cut(s)	380
BstC8I	GCNNGC	7 cut(s)	502, 506, 510, 670, 1066, 1572, 1667
BstDEI	CTNAG	9 cut(s)	12, 139, 153, 417, 738, 843, 867, 1265, 1472
BstF5I	GGATG	6 cut(s)	215, 288, 931, 1343, 1466, 1787
BstFNI	CGCG	1 cut(s)	882
BstH2I	RGCGCY	1 cut(s)	1071
BstHHI	GCGC	1 cut(s)	1070
BstKTI	GATC	9 cut(s)	415, 492, 790, 853, 1158, 1264, 1361, 1453, 1916
BstMAI	GTCTC	5 cut(s)	209, 215, 992, 1610, 1768
BstMBI	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
BstMCI	CGRYCG	1 cut(s)	1506
BstMWI	GCNNNNNNNGC	4 cut(s)	380, 954, 1508, 1760
BstNI	CCWGG	4 cut(s)	599, 685, 995, 1817
BstNSI	RCATGY	2 cut(s)	508, 1534
BstPAI	GACNNNNGTC	1 cut(s)	542
BstSCI	CCNGG	5 cut(s)	597, 683, 993, 1815, 1821
BstSFI	CTRYAG	4 cut(s)	1314, 1335, 1702, 1875
BstUI	CGCG	1 cut(s)	882
BstV1I	GCAGC	5 cut(s)	1320, 1349, 1352, 1495, 1849
BstV2I	GAAGAC	3 cut(s)	542, 972, 1418
BstX2I	RGATCY	2 cut(s)	489, 1450
BstYI	RGATCY	2 cut(s)	489, 1450
BstZI	CGGCCG	1 cut(s)	1503
Bsu15I	ATCGAT	1 cut(s)	790
BsuRI	GGCC	7 cut(s)	180, 455, 1011, 1424, 1505, 1570, 1609
BsuTUI	ATCGAT	1 cut(s)	790
BtrI	CACGTC	1 cut(s)	1416
BtsCI	GGATG	6 cut(s)	215, 288, 931, 1343, 1466, 1787
BtsI	GCAGTG	1 cut(s)	1206
BtsIMutI	CAGTG	4 cut(s)	147, 672, 1206, 1864
BveI	ACCTGC	2 cut(s)	1465, 1698
Cac8I	GCNNGC	7 cut(s)	502, 506, 510, 670, 1066, 1572, 1667
CaiI	CAGNNNCTG	1 cut(s)	1713
CciNI	GCGGCCGC	1 cut(s)	1503
CfoI	GCGC	1 cut(s)	1070
Cfr10I	RCCGGY	1 cut(s)	1562
Cfr13I	GGNCC	3 cut(s)	1009, 1607, 1819
ClaI	ATCGAT	1 cut(s)	790
CsiI	ACCWGGT	1 cut(s)	1815
Csp6I	GTAC	5 cut(s)	149, 227, 271, 448, 1354
CspAI	ACCGGT	1 cut(s)	1562
CviQI	GTAC	5 cut(s)	149, 227, 271, 448, 1354
DdeI	CTNAG	9 cut(s)	12, 139, 153, 417, 738, 843, 867, 1265, 1472
DpnI	GATC	9 cut(s)	414, 491, 789, 852, 1157, 1263, 1360, 1452, 1915
DpnII	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
DraIII	CACNNNGTG	1 cut(s)	1859
DrdI	GACNNNNNNGTC	1 cut(s)	1945
DriI	GACNNNNNGTC	1 cut(s)	1781
DseDI	GACNNNNNNGTC	1 cut(s)	1945
EaeI	YGGCCR	1 cut(s)	1503
EagI	CGGCCG	1 cut(s)	1503
Eam1104I	CTCTTC	1 cut(s)	214
Eam1105I	GACNNNNNGTC	1 cut(s)	1781
EarI	CTCTTC	1 cut(s)	214
Ecl136II	GAGCTC	1 cut(s)	1728
EclXI	CGGCCG	1 cut(s)	1503
Eco130I	CCWWGG	2 cut(s)	1347, 1675
Eco147I	AGGCCT	1 cut(s)	455
Eco24I	GRGCYC	1 cut(s)	1730
Eco31I	GGTCTC	1 cut(s)	215
Eco32I	GATATC	1 cut(s)	94
Eco47I	GGWCC	1 cut(s)	1819
Eco52I	CGGCCG	1 cut(s)	1503
Eco53kI	GAGCTC	1 cut(s)	1728
Eco57I	CTGAAG	2 cut(s)	996, 1095
Eco88I	CYCGRG	1 cut(s)	1721
EcoICRI	GAGCTC	1 cut(s)	1728
EcoRII	CCWGG	4 cut(s)	597, 683, 993, 1815
EcoRV	GATATC	1 cut(s)	94
EcoT14I	CCWWGG	2 cut(s)	1347, 1675
EcoT38I	GRGCYC	1 cut(s)	1730
ErhI	CCWWGG	2 cut(s)	1347, 1675
FalI	AAGNNNNNCTT	2 cut(s)	974, 1006
FaqI	GGGAC	2 cut(s)	320, 371
FauNDI	CATATG	1 cut(s)	391
FblI	GTMKAC	2 cut(s)	823, 1552
Fnu4HI	GCNGC	7 cut(s)	1309, 1338, 1341, 1503, 1506, 1509, 1863
FokI	GGATG	6 cut(s)	202, 275, 938, 1330, 1453, 1774
FriOI	GRGCYC	1 cut(s)	1730
Fsp4HI	GCNGC	7 cut(s)	1309, 1338, 1341, 1503, 1506, 1509, 1863
FspBI	CTAG	2 cut(s)	1125, 1287
GlaI	GCGC	1 cut(s)	1069
GluI	GCNGC	7 cut(s)	1309, 1338, 1341, 1503, 1506, 1509, 1863
GsuI	CTGGAG	1 cut(s)	581
HaeII	RGCGCY	1 cut(s)	1071
HaeIII	GGCC	7 cut(s)	180, 455, 1011, 1424, 1505, 1570, 1609
HapII	CCGG	3 cut(s)	1563, 1791, 1822
HhaI	GCGC	1 cut(s)	1070
Hin6I	GCGC	1 cut(s)	1068
HinP1I	GCGC	1 cut(s)	1068
HincII	GTYRAC	2 cut(s)	547, 988
HindII	GTYRAC	2 cut(s)	547, 988
HinfI	GANTC	5 cut(s)	48, 74, 1106, 1525, 1580
HpaII	CCGG	3 cut(s)	1563, 1791, 1822
HphI	GGTGA	2 cut(s)	43, 635
Hpy166II	GTNNAC	4 cut(s)	547, 824, 988, 1553
Hpy188III	TCNNGA	5 cut(s)	416, 740, 755, 1050, 1125
Hpy8I	GTNNAC	4 cut(s)	547, 824, 988, 1553
Hpy99I	CGWCG	1 cut(s)	1894
HpyAV	CCTTC	1 cut(s)	1414
HpyCH4IV	ACGT	4 cut(s)	276, 1100, 1415, 1892
HpyF10VI	GCNNNNNNNGC	4 cut(s)	380, 954, 1508, 1760
HpyF3I	CTNAG	9 cut(s)	12, 139, 153, 417, 738, 843, 867, 1265, 1472
HpySE526I	ACGT	4 cut(s)	276, 1100, 1415, 1892
HspAI	GCGC	1 cut(s)	1068
Kzo9I	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
LmnI	GCTCC	4 cut(s)	16, 1148, 1598, 1662
Lsp1109I	GCAGC	5 cut(s)	1320, 1349, 1352, 1495, 1849
LweI	GCATC	5 cut(s)	655, 1055, 1231, 1352, 1750
MabI	ACCWGGT	1 cut(s)	1815
MaeI	CTAG	2 cut(s)	1125, 1287
MaeII	ACGT	4 cut(s)	276, 1100, 1415, 1892
MaeIII	GTNAC	7 cut(s)	1161, 1330, 1364, 1715, 1783, 1893, 1936
MalI	GATC	9 cut(s)	414, 491, 789, 852, 1157, 1263, 1360, 1452, 1915
MboI	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
MflI	RGATCY	2 cut(s)	489, 1450
MhlI	GDGCHC	2 cut(s)	1730, 1807
MlyI	GAGTC	2 cut(s)	1100, 1574
MmeI	TCCRAC	1 cut(s)	588
MroXI	GAANNNNTTC	1 cut(s)	1281
MseI	TTAA	9 cut(s)	131, 282, 638, 834, 860, 981, 1224, 1394, 1907
MslI	CAYNNNNRTG	1 cut(s)	1833
MspA1I	CMGCKG	3 cut(s)	916, 1508, 1919
MspI	CCGG	3 cut(s)	1563, 1791, 1822
MspR9I	CCNGG	5 cut(s)	599, 685, 995, 1817, 1823
MvaI	CCWGG	4 cut(s)	599, 685, 995, 1817
MvnI	CGCG	1 cut(s)	882
MwoI	GCNNNNNNNGC	4 cut(s)	380, 954, 1508, 1760
NciI	CCSGG	1 cut(s)	1823
NdeI	CATATG	1 cut(s)	391
NdeII	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
NlaIV	GGNNCC	3 cut(s)	931, 1150, 1886
NmuCI	GTSAC	4 cut(s)	1161, 1715, 1783, 1936
NotI	GCGGCCGC	1 cut(s)	1503
NspI	RCATGY	2 cut(s)	508, 1534
PaeI	GCATGC	1 cut(s)	508
PaqCI	CACCTGC	1 cut(s)	1465
PceI	AGGCCT	1 cut(s)	455
PciI	ACATGT	1 cut(s)	1530
PdmI	GAANNNNTTC	1 cut(s)	1281
PfeI	GAWTC	3 cut(s)	48, 74, 1525
PfoI	TCCNGGA	1 cut(s)	993
PinAI	ACCGGT	1 cut(s)	1562
PkrI	GCNGC	7 cut(s)	1310, 1339, 1342, 1504, 1507, 1510, 1864
PleI	GAGTC	2 cut(s)	1100, 1574
PpsI	GAGTC	2 cut(s)	1100, 1574
PscI	ACATGT	1 cut(s)	1530
PshAI	GACNNNNGTC	1 cut(s)	542
PshBI	ATTAAT	1 cut(s)	131
Psp124BI	GAGCTC	1 cut(s)	1730
Psp6I	CCWGG	4 cut(s)	597, 683, 993, 1815
PspGI	CCWGG	4 cut(s)	597, 683, 993, 1815
PspN4I	GGNNCC	3 cut(s)	931, 1150, 1886
PspPI	GGNCC	3 cut(s)	1009, 1607, 1819
PstI	CTGCAG	1 cut(s)	1339
PstNI	CAGNNNCTG	1 cut(s)	1713
PsuI	RGATCY	2 cut(s)	489, 1450
PvuII	CAGCTG	2 cut(s)	916, 1919
RsaI	GTAC	5 cut(s)	150, 228, 272, 449, 1355
RsaNI	GTAC	5 cut(s)	149, 227, 271, 448, 1354
RseI	CAYNNNNRTG	1 cut(s)	1833
SacI	GAGCTC	1 cut(s)	1730
SaqAI	TTAA	9 cut(s)	131, 282, 638, 834, 860, 981, 1224, 1394, 1907
SatI	GCNGC	7 cut(s)	1309, 1338, 1341, 1503, 1506, 1509, 1863
Sau3AI	GATC	9 cut(s)	412, 489, 787, 850, 1155, 1261, 1358, 1450, 1913
Sau96I	GGNCC	3 cut(s)	1009, 1607, 1819
SchI	GAGTC	2 cut(s)	1100, 1574
ScrFI	CCNGG	5 cut(s)	599, 685, 995, 1817, 1823
SduI	GDGCHC	2 cut(s)	1730, 1807
SexAI	ACCWGGT	1 cut(s)	1815
SfaNI	GCATC	5 cut(s)	655, 1055, 1231, 1352, 1750
SfcI	CTRYAG	4 cut(s)	1314, 1335, 1702, 1875
SinI	GGWCC	1 cut(s)	1819
SmiMI	CAYNNNNRTG	1 cut(s)	1833
SmlI	CTYRAG	1 cut(s)	581
SmoI	CTYRAG	1 cut(s)	581
SphI	GCATGC	1 cut(s)	508
SseBI	AGGCCT	1 cut(s)	455
SsiI	CCGC	2 cut(s)	1502, 1506
SspMI	CTAG	2 cut(s)	1125, 1287
SstI	GAGCTC	1 cut(s)	1730
StuI	AGGCCT	1 cut(s)	455
StyD4I	CCNGG	5 cut(s)	597, 683, 993, 1815, 1821
StyI	CCWWGG	2 cut(s)	1347, 1675
TaiI	ACGT	4 cut(s)	279, 1103, 1418, 1895
TaqI	TCGA	3 cut(s)	790, 921, 1239
TaqII	GACCGA	1 cut(s)	1203
TauI	GCSGC	2 cut(s)	1505, 1508
TfiI	GAWTC	3 cut(s)	48, 74, 1525
Tru1I	TTAA	9 cut(s)	131, 282, 638, 834, 860, 981, 1224, 1394, 1907
Tru9I	TTAA	9 cut(s)	131, 282, 638, 834, 860, 981, 1224, 1394, 1907
TscAI	CASTG	4 cut(s)	147, 679, 1213, 1864
TseFI	GTSAC	4 cut(s)	1161, 1715, 1783, 1936
TseI	GCWGC	5 cut(s)	1308, 1337, 1340, 1508, 1862
Tsp45I	GTSAC	4 cut(s)	1161, 1715, 1783, 1936
TspDTI	ATGAA	8 cut(s)	201, 347, 642, 681, 954, 1380, 1424, 1761
TspGWI	ACGGA	3 cut(s)	104, 1107, 1371
TspRI	CASTG	4 cut(s)	147, 679, 1213, 1864
VpaK11BI	GGWCC	1 cut(s)	1819
VspI	ATTAAT	1 cut(s)	131
XapI	RAATTY	4 cut(s)	475, 830, 1280, 1494
XbaI	TCTAGA	1 cut(s)	1124
XceI	RCATGY	2 cut(s)	508, 1534
XmiI	GTMKAC	2 cut(s)	823, 1552
XmnI	GAANNNNTTC	1 cut(s)	1281
XspI	CTAG	2 cut(s)	1125, 1287

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.