Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007772

Physical Location & Seq

Genomic Coordinates Reverse (-)

32061709 .. 32077665

Gene Structure 15957 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1232200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6054 bp

ATGTCGGAGGAGGAGAAGTTGTTGAAGGAGGCGAAGAAACTGCCGTGGGATGATCGGCTCTTCCACAAGAACTGGAAGGTGAGGAACGAGGCCAACATTGACTTGGCCGCTCTCTGCGACTCCGTTGCCGATCCCAAAGACTCTCGCCTCCGTGAATTTGGTCCGCTTTTTCGGAAGACTTTGGCGGATTCCAATTCGCCTGTGCAAGAGAAGGCCCTCGATGCATTGATTGCATTTTTACGAGCTGCGGATGCTGATGCCGGAAGATTTGCCAAGGAAGTTTGTGATGCCATTGTGGCGAAATGCCTCACTGGTAGGCCGAAGACGGTGGAAAAGGCTCAAGCTGCATTTATGCTTTGGGTTGAATTGGAGGCCGTGGACGCTTTCTTGGATGCTATGGAGAAGGCAATAAAAAACAAAGTTTCCAAAGCTGTTGTGCCTGCAATTGATGTCATGTTTCAGGCCTTAAGTGAATTTGGCGCAAAAGTTGTTCCTCCAAAAAGGATTTTGAAGATGCTCCCCGAATTATTTGACCATCAAGATCAGAATGTTCGTGCATGTTCCAAAGGGCTTACTCTTGAGCTCTGCCGTTGGATAGGAAAGGACCCTGTTAAATCAATATTGTTTGAGAAGATGAGGGATACAATGAAAAAAGAGTTGGAGGCTGAGCTTGTAAATGTTACAGGGACAGCCAGGCCATCTCGCAAAATCAGATCGGAGCAAGACAAGGAGCCAGAAAAGGAAGCTGTTTCTGAAGTAGTTTGCCCTGGTCCTTTGGAGGAATCTGCAGCAGATGCTCCTCAGGAAATAGATGAATATGAGCTTGTTGATCCGGTTGATATATTGACTCCTCTGGAGAAGTCTGGATTTTGGGACGGAGTGAAAGCTACAAAGTGGTCAGAGCGAAAGGAGGCTGTTGCAGAGCTAACCAAGCTTGCTTCAACTAAAAGAATTGCCCCTGGCGATTTTACAGAAATCTGTCGGACATTAAAGAAGCTTGTAACAGATGTGAACATAGCTGTTGCAGTTGAAGCTATTCAAGCTATTGGTAATCTTGCTAAGGGTCTAAGGACTCATTTTTCTGGAAGTTCACGATTTCTATTGCCAGGTTTACTTGAAAAATTGAAAGAAAAAAAGCCAACTATGACTGAGGCACTCTCTCAAACTCTTCAAGCAATGCATACTGCTGGCTGCTTAAATCTCATTGACATTGCTGAAGATATTAAGACAGCAGTAAAAAATAAAGTACCCCTTGTGCGTTCATTGACTCTGAACTGGGTGACATTTTGTATTGAAACAAGCAACAAGGCTGTTGTTTTAAAGTTACACAAGGACTACGTTCCTATATTTATGGAGTGCCTCAATGATGGAACCCCAGAAGTGAGGGATGCATCCTTTTCAGCTTTGACAGCAATAGCTAAGGTAATGGTTGGTATGAGGCCCTTGGAAAGGTCACTTGAGAAACTTGATGATGTCAGAAGAAAGAAGCTCTCTGAAATGATTATGGGTTCTGAAGGTGGTGCATCCACTACTGCAAACTCAGCCCAAAGCTCTGGTGTGACTGCACCCTCTTTGGAGACTACGGACAGTGCGTTTGTTCGAAGGTCAGCTGCAAGCATGCTTAGTGGGAAGAGGCCTGTTCAAGCAGCTCCTGCAAAACAAAAAGCGGGATCTGTTAAATTGGGTGGCAGTAAGAAAGGAGATGCAACTGTGCAGCCAAAAGCTTCTAAGTTAGTTGAAACCCCTGAAGATGTTGAGCCAGGAGAAATGAGTCTTGAAGAGATTGAAAGCAGATTAGGCTCTCTTATACAAGAAGAAACTGTCTCTCAATTAAAAAGTAGCGCATGGAAAGAGCGGCTTGAAGCCATATCCTCATTTAGACAGCAAGTAGAAGGTTTACAGGACATTGACCTGTCAATTGAGATGTTAGTTCGTTTACTTTGTGCTGTCCCTGGTTGGAGTGAAAAAAATGTTCAGGTTCAGCAACAGGTTATTGAAGTTATTACCTATATGGCGTCCACTGCAAAGAAGTTTCCTAAGAAATGTGTAGTACTTTGTCTTCTGGGTATTAGTGAACGGGTTGCAGATATCAAGACTCGGACTCATGCCATGAAGTGCCTCACTGCTTTTTCTGAAGCAATAGGTCCAGGATTAGTTTTTGAAAGACTTTATAAAATTATGAAAGAGCACAAGAACCCTAAGGTTCTCAGCGAGGGAGTACTGTGGATGGTTTCGGCAGTTGAGGACTTTGGTGTCTCACATGTGAAACTTAAGGATTTGATTGATTTTTGTAAAGAAACTGGACTTCAGTCCAGTGTTGCTGCCACTAGGAACTCTACTATTAAGCTTTTGGGTGCTATACATAAATTTGTTGGTCCAGATATTAAAGGGTTTCTTACTGATGTTAAACCTGCACTACTCAGTGCACTGGACACGGAGTATGAAAAGAATCCATTTGAGGGTGCTTCTGTGGTTCTGAAGAGAAGTGTTAGGGCAACGGAATCTACTTCATCTGTCTCTGTAGGCGGGTTAGATGGCCTTCCACGTGAAGATATTAGTGGAAAGGTCACACCTACTCTGCTGAAGAGCTTGGAGAGTCCTGATTGGAAGGTTCGCTTGGAGTCAATAGAAGCCGTCGATAAGATTTTAGAAGAGGCTAATAAGCGCATCCAGCCAACTGGAACTGCGGAGTTGTTTGGTGCCTTAAGGGGGCGCTTATATGATAGCAATAAGAACTTGGTCGCTGCAACCTTAACGGTTGTTGGTCATGTTGCTTCTGCAATGGGCGCACCAGTTGAAAAGTTCAGCAAGGGCTTTCTGTCGGATGTTTTGAAATGTCTAGGAGACAATAAGAAGCATATGAGAGAATGCACTTTAACTGCTTTAGACTCGTGGCTCTCTGCTGTTCATCTTGATAAAATGGTTCCTTATATTACAGCAGCTATATCAGATGCCAAACTTGGTGCAGAAGGGCGGAAAGATCTTTTCGAGTGGTTGACAAGACAGCTTTCTGGGTTAAGTGAATTCTCTGATGCTGCTCATCTTTTGAAACCAGCTTCCTCTGCTTTGACGGATAAATCCTCAGATGTTCGTAAAGCAGCAGAGACATGTGTTTCTGAAATTTTGAGAGTTAGTGGGCAAGAAACGGTTGAGAAGATTTTGAGAGATATTCATGGGCCAACTTTAGCTCTTGTTGAACGATTGAAGCCTAATGGATCTTTTCAAGAATCTTTTGAGTCAGCCAAAGCAATATCGATGGCCCCAACTTCCAAAAGCATTACAAAGGCTGGAAAGCCTGCTTCCAATGGAGTTTTGAAGCATGGAGCCAAAGCTACTTCAAGGACCATTCCCACAAAGGGATTAAATTCAATGATGTCCGTCCAAGATATAGCTGTGCAGTCACAGGCCTTGATAAATGTTAAAGATTCAATTAAGGACGATAGAGAGCGATTGGTAGCTAAGAAGTTTAAGTTTGAAGAGCCTCGGATAGAACAGATTCAAGATCTTGAGAATGATATGACGAAGTACTTTAGAGAGGATGTGCATAGGCGGCTTCTTTCTACTGATTTTAAGAAGCAAGTTGATGGCCTTGAGATACTACAGAAGGCACTTCCAACCATTAAAAAGGAAATTATTGAAGTATTGGATATACTGCTAAGGTGGTTTGTTTTACAATTTTGTAAATCGAACACAACATGCCTGTTAAAGGTGTTGGAATTTCTTCCAGAACTTTTTGATACATTTAGGGATGAGGCATACATGTTGACTGAATCAGAAGCAGCCATATTTTTTCCATGCTTGATAGAGAAGCTCGGGCATAACATTGAGAAAGTGCGAGAAAAAATGCGGGAGTTGACCAAACAAATTGTGCAGGCATATACCGCTGCAAAAAGTTTCCCTTATATTTTGGAGGGATTGCGTTCTAAGAACAACCGTACTCGAATTGAGTGTGCTGATCTTGTTGGATATTTGATTGATCACCATGTAGCTGAGATTAGTGGACAACTAAAGTCCTTGCAAATTGTTGCGAGCTTGACAGCAGAAAGAGATGGTGAAATTAGGAAAGCTGCTTTAAATACGCTAGCTACTGGTTATAAGATTCTTGGTGAGGACATATGGAGATATGTCGGAAAGTTAACAGATGCTCAGAAAAGCATGCTAGATGATCGATTTAAGTGGAAGGTTCGAGAAATGGAGAAAAGGAAGGAAGGAAAGCCTGGCGAAGCTAGGGCTTCTCTGAGGCGTTCTGTCAGGGAAATTGGGCTGGATGTAGCAGAGCAAAGCGGGGAGGTTGCACGATCTGTTTCTGGTCCAATGCTTTCTAGGAGAAACTATGGCCATCCTGAGCCTCACATGGAGAGGCAGCTGGTGCCACCCAATGTACTCAGTAGTGCCAATGGCCCCACAGACTGGAATGAAGCTTTGGATATCATTTCTTTTGGTTCTCCTGAGCAGTCAGTTGAAGGAATGAAAGTTGTGTGTCATGAGTTGGCACAGGCGACTAATGATCCAGAAGGCAGTGCTATGGATGAACTAGTGCGGGATTCTGATAGACTTGTTTCTTGCTTGGCAAATAAGGTAGCAAAGACTTTTGACTTCAGTTTGACGGGAGCTTCGTCTAGGTCATGTAAATATGTCTTGAACACTCTTATGCAGACATTTCAAAATAAAAGACTTGCTTATGCTGTCAAGGAAAGTACTCTTGACAGTCTAATCACTGAGCTCCTACTGTGGCTTTTGGATGAAAGGGTTCCCCATATGGATGATGGCAGCCAACTCCTGAAAGCCTTGAATGTTTTGATGCTAAAGATTCTGGATAATGCAGATCGGACTTCGTCCTTTGTTGTTCTCATCAACCTCTTACGCCCTTTAGATCCCTCAAGATGGCCATCACCTGCATCCAATGAATCATTTGCTTCCAGAAACCAGAAGTTCTCCGATTTGGTTGTCAAATGTTTAATAAAACTTACAAAGGTTCTTCAAAGCACAATATATGATGTCGATCTGGACCGTATCCTTCAAAGCATCCATTTATACCTACAAGACTTAGGGATGGAAGAGATTAGGAGAAGAGCTGGTGCTGATGACAAACCACTGCGTATGGTGAAGACTGTTCTACATGAGCTTGTGAAGCTTCGTGGAGCTGCAATTAAAGGTCACCTTTCCATGGTTCCTATAGACGTAAAACCGCAGCCAATCATTCTTGCCTACATTGATCTTAATCTTGAGACTTTAGCTGCGGCAAGGATGCTGACATCAACTGGGTCTGGTGGCCAAACTCACTGGGGTGATTCAGCAGCAAACAATCCTTCATCTGCCACACATTCTGCTGATGCACAGTTAAAGCAAGAACTTGCTGCCATATTCAAGAAGATTGGCGACAAGCAAACCTGCACCATTGGTCTGTATGAACTATACCGCATAACCCAACTATACCCAAGAGTTGATATATTCTCTCAACTCCAAAATGCTAGCGAGGCTTTTCGGACATATATTAGAGATGGTTTGGCGCAGATGGAAAAGAATGCCGCAGCTGGAAGGACGCCTTCAAGTTTGCCAATGCCGACTCCTCCCCCAGCTACTCTAAATGCTTCTTCTCCTGAATTTGCACCCCTGTCCCCTGTACACACAAACTCCTTGCTTGATTCAAAATCATTAAATGTGAAATCCGAACCAACTAGCTTTAATCTACCGCCATCATATGATGAAGAAAACAGGCTTAATAATGCCATTACTTCTAGAGGTCTCACCGAAAACTCATTGGTAGACCAAAGGAATGAGAGATATATTGGTGGAGTGACCAACGGAACCTTAGATGCGATTAGAGAGAGGATGAAGAGCATGCAATTGGCAGCGGCTGCTGGGAACCCGGATCAAGAAACTAGGCCACTAATCTATGTGAATGACAACGTAAACCAGAGTCTCTCCGGTCAGATTCCTCGTGCATCAGAGATTCCCCTCCAGGGCGGGGTTCTCCCTATGGATGAGAGGGCATTATCCGGGCTTCAGGCTCGGATGGAGAGACTAAAAAGTGGGACAATCGAACCCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000775 GO:0000776 GO:0000910 GO:0000911 GO:0000919 GO:0000922 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005911 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007163 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009574 GO:0009832 GO:0009920 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0030054 GO:0030863 GO:0030951 GO:0030952 GO:0030981 GO:0031109 GO:0032506 GO:0032991 GO:0034622 GO:0042546 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046785 GO:0048285 GO:0051010 GO:0051258 GO:0051301 GO:0055044 GO:0061640 GO:0065003 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0097435 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT

Protein Analysis

2018

Amino Acids

222.78

Weight (kDa)

7.9

Isoelectric Point (pI)

45.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XMAP215_CLASP_TOG	PF21041	11 - 223	5.3e-61	XMAP215/Dis1/CLASP, TOG domain
XMAP215_CLASP_TOG	PF21041	297 - 499	1.1e-06	XMAP215/Dis1/CLASP, TOG domain
CLASP_N	PF12348	298 - 473	9.1e-09	CLASP N terminal
XMAP215_CLASP_TOG	PF21041	610 - 813	9.7e-12	XMAP215/Dis1/CLASP, TOG domain
XMAP215_CLASP_TOG	PF21041	1184 - 1391	2.4e-06	XMAP215/Dis1/CLASP, TOG domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0007772)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35630 AT2G35630
fragaria_vesca	FvH4_7g33390 FvH4_7g33390 FvH4_7g33390
malus_domestica	MD01G1232200.v1.1 MD07G1304000.v1.1
prunus_persica	Prupe.1G083500_v2.0.a1 Prupe.1G083500_v2.0.a1 Prupe.1G083500_v2.0.a1 Prupe.1G083500_v2.0.a1
pyrus_communis	pycom01g24060
rosa_chinensis	RchiOBHm_Chr1g0383361
rosa_laevigata	RLG00000026109
rosa_multiflora	Rmu_sc0007173.1_g000012
rosa_roxburghii	Rroxscaffold_4G00277360
rosa_rugosa	Rorug01G0440800 Rorug01G0440800 Rorug01G0440900
rosa_samantha	Rh1AG466800 Rh1BG424100 Rh1CG437200 Rh1DG456800
rosa_wichuraiana	Rw1G040580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2172, 4095
AarI	CACCTGC	1 cut(s)	4903
AasI	GACNNNNNNGTC	1 cut(s)	2252
Acc36I	ACCTGC	3 cut(s)	2419, 4903, 5399
AccB1I	GGYRCC	2 cut(s)	2699, 4369
AccB7I	CCANNNNNTGG	1 cut(s)	4410
AccBSI	CCGCTC	2 cut(s)	110, 1855
AccI	GTMKAC	1 cut(s)	5766
AclWI	GGATC	7 cut(s)	125, 824, 1678, 3223, 4502, 4868, 5880
AcoI	YGGCCR	4 cut(s)	105, 4336, 4886, 5272
AcsI	RAATTY	8 cut(s)	155, 473, 2366, 3023, 3120, 3364, 3716, 5603
AcvI	CACGTG	1 cut(s)	2546
AcyI	GRCGYC	2 cut(s)	2015, 5543
AfaI	GTAC	8 cut(s)	1248, 2052, 2220, 3527, 3937, 4383, 4699, 5625
AflII	CTTAAG	3 cut(s)	466, 2270, 2704
AflIII	ACRYGT	3 cut(s)	2260, 3107, 3759
AhlI	ACTAGT	1 cut(s)	4534
AjnI	CCWGG	9 cut(s)	692, 766, 958, 1105, 1759, 1951, 2146, 4216, 5961
AjuI	GAANNNNNNNTTGG	6 cut(s)	641, 673, 2600, 2632, 4406, 4438
AleI	CACNNNNGTG	1 cut(s)	5286
Alw21I	GWGCWC	4 cut(s)	585, 2190, 2428, 4725
Alw44I	GTGCAC	1 cut(s)	2424
AlwI	GGATC	7 cut(s)	125, 824, 1678, 3223, 4502, 4868, 5880
AlwNI	CAGNNNCTG	2 cut(s)	1652, 5858
Ama87I	CYCGRG	1 cut(s)	3812
ApaLI	GTGCAC	1 cut(s)	2424
ApoI	RAATTY	8 cut(s)	155, 473, 2366, 3023, 3120, 3364, 3716, 5603
ArsI	GACNNNNNNTTYG	4 cut(s)	1577, 1609, 4597, 4629
Asp700I	GAANNNNTTC	4 cut(s)	1035, 3495, 3720, 4749
AspLEI	GCGC	6 cut(s)	482, 1844, 2667, 2715, 2789, 5512
AsuC2I	CCSGG	2 cut(s)	5870, 6001
AsuII	TTCGAA	1 cut(s)	1600
AsuNHI	GCTAGC	2 cut(s)	4081, 5471
AvaI	CYCGRG	1 cut(s)	3812
AvaII	GGWCC	8 cut(s)	161, 604, 770, 2144, 2375, 3342, 4310, 5008
AxyI	CCTNAGG	2 cut(s)	801, 2199
BaeGI	GKGCMC	1 cut(s)	2428
BalI	TGGCCA	3 cut(s)	4338, 4888, 5274
BanI	GGYRCC	2 cut(s)	2699, 4369
BanII	GRGCYC	2 cut(s)	585, 4725
BarI	GAAGNNNNNNTAC	2 cut(s)	2043, 2075
BauI	CACGAG	2 cut(s)	2890, 5940
BbrPI	CACGTG	1 cut(s)	2546
BbsI	GAAGAC	4 cut(s)	182, 329, 2051, 5114
Bbv12I	GWGCWC	4 cut(s)	585, 2190, 2428, 4725
BceAI	ACGGC	4 cut(s)	28, 359, 573, 2618
BcgI	CGANNNNNNTGC	4 cut(s)	22, 56, 107, 141
BciT130I	CCWGG	9 cut(s)	694, 768, 960, 1107, 1761, 1953, 2148, 4218, 5963
BciVI	GTATCC	2 cut(s)	634, 5024
BclI	TGATCA	1 cut(s)	3976
BcnI	CCSGG	2 cut(s)	5870, 6001
BcuI	ACTAGT	1 cut(s)	4534
BfmI	CTRYAG	4 cut(s)	786, 2520, 3599, 5175
BfoI	RGCGCY	1 cut(s)	2716
BfrI	CTTAAG	3 cut(s)	466, 2270, 2704
BfuAI	ACCTGC	3 cut(s)	2419, 4903, 5399
BfuI	GTATCC	2 cut(s)	634, 5024
BglI	GCCNNNNNGGC	1 cut(s)	296
BglII	AGATCT	2 cut(s)	2980, 3502
BlpI	GCTNAGC	1 cut(s)	666
BmcAI	AGTACT	4 cut(s)	2052, 2220, 3527, 4699
Bme18I	GGWCC	8 cut(s)	161, 604, 770, 2144, 2375, 3342, 4310, 5008
BmeT110I	CYCGRG	1 cut(s)	3812
BmrI	ACTGGG	3 cut(s)	1285, 5271, 5293
BmtI	GCTAGC	2 cut(s)	4085, 5475
BmuI	ACTGGG	3 cut(s)	1285, 5271, 5293
BoxI	GACNNNNGTC	1 cut(s)	2308
BpiI	GAAGAC	4 cut(s)	182, 329, 2051, 5114
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
BpmI	CTGGAG	2 cut(s)	875, 5945
Bpu10I	CCTNAGC	5 cut(s)	1059, 1419, 3656, 4344, 4449
Bpu1102I	GCTNAGC	1 cut(s)	666
Bpu14I	TTCGAA	1 cut(s)	1600
BpuEI	CTTGAG	7 cut(s)	324, 599, 1478, 3527, 3611, 4864, 5246
BpuMI	CCSGG	2 cut(s)	5870, 6001
Bsa29I	ATCGAT	2 cut(s)	3254, 4168
BsaAI	YACGTR	1 cut(s)	2546
BsaBI	GATNNNNATC	3 cut(s)	4888, 5001, 5220
BsaHI	GRCGYC	2 cut(s)	2015, 5543
BsaI	GGTCTC	1 cut(s)	5750
BsaWI	WCCGGW	2 cut(s)	832, 5927
BsaXI	ACNNNNNCTCC	5 cut(s)	5, 32, 35, 5618, 5648
Bse21I	CCTNAGG	2 cut(s)	801, 2199
Bse3DI	GCAATG	3 cut(s)	1182, 1209, 2787
Bse8I	GATNNNNATC	3 cut(s)	4888, 5001, 5220
BseBI	CCWGG	9 cut(s)	694, 768, 960, 1107, 1761, 1953, 2148, 4218, 5963
BseCI	ATCGAT	2 cut(s)	3254, 4168
BseJI	GATNNNNATC	3 cut(s)	4888, 5001, 5220
BseMI	GCAATG	3 cut(s)	1182, 1209, 2787
BseRI	GAGGAG	5 cut(s)	23, 26, 789, 840, 5559
BseSI	GKGCMC	1 cut(s)	2428
BseYI	CCCAGC	2 cut(s)	5575, 5861
BsgI	GTGCAG	7 cut(s)	1548, 1733, 2397, 2985, 3416, 3890, 5377
BshNI	GGYRCC	2 cut(s)	2699, 4369
BshVI	ATCGAT	2 cut(s)	3254, 4168
BsiHKAI	GWGCWC	4 cut(s)	585, 2190, 2428, 4725
BsiHKCI	CYCGRG	1 cut(s)	3812
BsiSI	CCGG	5 cut(s)	261, 833, 5870, 5928, 6000
BslFI	GGGAC	5 cut(s)	700, 887, 1934, 5602, 6049
BsmFI	GGGAC	5 cut(s)	700, 887, 1934, 5602, 6049
BsmI	GAATGC	2 cut(s)	2873, 5530
Bso31I	GGTCTC	1 cut(s)	5750
BsoBI	CYCGRG	1 cut(s)	3812
Bsp119I	TTCGAA	1 cut(s)	1600
Bsp1286I	GDGCHC	4 cut(s)	585, 2190, 2428, 4725
Bsp1407I	TGTACA	1 cut(s)	5623
Bsp1720I	GCTNAGC	1 cut(s)	666
Bsp19I	CCATGG	1 cut(s)	5166
BspDI	ATCGAT	2 cut(s)	3254, 4168
BspHI	TCATGA	1 cut(s)	4483
BspMAI	CTGCAG	1 cut(s)	790
BspMI	ACCTGC	3 cut(s)	2419, 4903, 5399
BspOI	GCTAGC	2 cut(s)	4085, 5475
BspPI	GGATC	7 cut(s)	125, 824, 1678, 3223, 4502, 4868, 5880
BspQI	GCTCTTC	5 cut(s)	65, 2579, 3471, 5065, 5831
BspT104I	TTCGAA	1 cut(s)	1600
BspT107I	GGYRCC	2 cut(s)	2699, 4369
BspTI	CTTAAG	3 cut(s)	466, 2270, 2704
BspTNI	GGTCTC	1 cut(s)	5750
BsrBI	CCGCTC	2 cut(s)	110, 1855
BsrDI	GCAATG	3 cut(s)	1182, 1209, 2787
BsrGI	TGTACA	1 cut(s)	5623
BssNI	GRCGYC	2 cut(s)	2015, 5543
BssSI	CACGAG	2 cut(s)	2890, 5940
BssT1I	CCWWGG	3 cut(s)	273, 1443, 5166
Bst2BI	CACGAG	2 cut(s)	2890, 5940
Bst2UI	CCWGG	9 cut(s)	694, 768, 960, 1107, 1761, 1953, 2148, 4218, 5963
BstACI	GRCGYC	2 cut(s)	2015, 5543
BstAFI	CTTAAG	3 cut(s)	466, 2270, 2704
BstAPI	GCANNNNNTGC	5 cut(s)	230, 794, 1652, 4369, 5858
BstAUI	TGTACA	1 cut(s)	5623
BstBAI	YACGTR	1 cut(s)	2546
BstBI	TTCGAA	1 cut(s)	1600
BstDSI	CCRYGG	3 cut(s)	44, 375, 5166
BstEII	GGTNACC	1 cut(s)	5156
BstENI	CCTNNNNNAGG	2 cut(s)	1447, 3704
BstH2I	RGCGCY	1 cut(s)	2716
BstHHI	GCGC	6 cut(s)	482, 1844, 2667, 2715, 2789, 5512
BstNI	CCWGG	9 cut(s)	694, 768, 960, 1107, 1761, 1953, 2148, 4218, 5963
BstNSI	RCATGY	8 cut(s)	561, 1621, 2264, 3111, 3699, 3763, 4159, 5845
BstPAI	GACNNNNGTC	1 cut(s)	2308
BstPI	GGTNACC	1 cut(s)	5156
BstSFI	CTRYAG	4 cut(s)	786, 2520, 3599, 5175
BstSLI	GKGCMC	1 cut(s)	2428
BstV2I	GAAGAC	4 cut(s)	182, 329, 2051, 5114
BstX2I	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
BstXI	CCANNNNNNTGG	2 cut(s)	1553, 2678
BstYI	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
Bsu15I	ATCGAT	2 cut(s)	3254, 4168
Bsu36I	CCTNAGG	2 cut(s)	801, 2199
BsuI	GTATCC	2 cut(s)	634, 5024
BsuTUI	ATCGAT	2 cut(s)	3254, 4168
BtgI	CCRYGG	3 cut(s)	44, 375, 5166
BtsI	GCAGTG	4 cut(s)	2019, 2121, 4525, 5093
BveI	ACCTGC	3 cut(s)	2419, 4903, 5399
CaiI	CAGNNNCTG	2 cut(s)	1652, 5858
CciI	TCATGA	1 cut(s)	4483
CfoI	GCGC	6 cut(s)	482, 1844, 2667, 2715, 2789, 5512
ClaI	ATCGAT	2 cut(s)	3254, 4168
CseI	GACGC	3 cut(s)	389, 2004, 5551
Csp6I	GTAC	8 cut(s)	1247, 2051, 2219, 3526, 3936, 4382, 4698, 5624
CviQI	GTAC	8 cut(s)	1247, 2051, 2219, 3526, 3936, 4382, 4698, 5624
DraI	TTTAAA	2 cut(s)	1320, 4074
DrdI	GACNNNNNNGTC	1 cut(s)	2252
DseDI	GACNNNNNNGTC	1 cut(s)	2252
EaeI	YGGCCR	4 cut(s)	105, 4336, 4886, 5272
EciI	GGCGGA	2 cut(s)	200, 2989
Ecl136II	GAGCTC	2 cut(s)	583, 4723
Eco130I	CCWWGG	3 cut(s)	273, 1443, 5166
Eco147I	AGGCCT	3 cut(s)	464, 1636, 3407
Eco24I	GRGCYC	2 cut(s)	585, 4725
Eco31I	GGTCTC	1 cut(s)	5750
Eco32I	GATATC	2 cut(s)	2089, 4429
Eco47I	GGWCC	8 cut(s)	161, 604, 770, 2144, 2375, 3342, 4310, 5008
Eco53kI	GAGCTC	2 cut(s)	583, 4723
Eco72I	CACGTG	1 cut(s)	2546
Eco81I	CCTNAGG	2 cut(s)	801, 2199
Eco88I	CYCGRG	1 cut(s)	3812
Eco91I	GGTNACC	1 cut(s)	5156
EcoICRI	GAGCTC	2 cut(s)	583, 4723
EcoNI	CCTNNNNNAGG	2 cut(s)	1447, 3704
EcoO109I	RGGNCCY	3 cut(s)	214, 604, 1440
EcoO65I	GGTNACC	1 cut(s)	5156
EcoRI	GAATTC	1 cut(s)	3023
EcoRII	CCWGG	9 cut(s)	692, 766, 958, 1105, 1759, 1951, 2146, 4216, 5961
EcoRV	GATATC	2 cut(s)	2089, 4429
EcoT14I	CCWWGG	3 cut(s)	273, 1443, 5166
EcoT22I	ATGCAT	3 cut(s)	226, 1182, 1393
EcoT38I	GRGCYC	2 cut(s)	585, 4725
ErhI	CCWWGG	3 cut(s)	273, 1443, 5166
FaqI	GGGAC	5 cut(s)	700, 887, 1934, 5602, 6049
FauI	CCCGC	6 cut(s)	1660, 2519, 3840, 4277, 4533, 5960
FauNDI	CATATG	4 cut(s)	2859, 4115, 4758, 5701
FbaI	TGATCA	1 cut(s)	3976
FblI	GTMKAC	1 cut(s)	5766
FriOI	GRGCYC	2 cut(s)	585, 4725
GlaI	GCGC	6 cut(s)	481, 1843, 2666, 2714, 2788, 5511
GsaI	CCCAGC	2 cut(s)	5579, 5865
GsuI	CTGGAG	2 cut(s)	875, 5945
HaeII	RGCGCY	1 cut(s)	2716
HapII	CCGG	5 cut(s)	261, 833, 5870, 5928, 6000
HgaI	GACGC	3 cut(s)	389, 2004, 5551
HhaI	GCGC	6 cut(s)	482, 1844, 2667, 2715, 2789, 5512
Hin1I	GRCGYC	2 cut(s)	2015, 5543
Hin6I	GCGC	6 cut(s)	480, 1842, 2665, 2713, 2787, 5510
HinP1I	GCGC	6 cut(s)	480, 1842, 2665, 2713, 2787, 5510
HincII	GTYRAC	4 cut(s)	2997, 3765, 3855, 4137
HindII	GTYRAC	4 cut(s)	2997, 3765, 3855, 4137
HindIII	AAGCTT	6 cut(s)	932, 995, 1722, 2345, 4419, 5132
HpaI	GTTAAC	1 cut(s)	4137
HpaII	CCGG	5 cut(s)	261, 833, 5870, 5928, 6000
Hpy99I	CGWCG	1 cut(s)	2639
HpyCH4IV	ACGT	4 cut(s)	1338, 2545, 5181, 5910
HpySE526I	ACGT	4 cut(s)	1338, 2545, 5181, 5910
Hsp92I	GRCGYC	2 cut(s)	2015, 5543
HspAI	GCGC	6 cut(s)	480, 1842, 2665, 2713, 2787, 5510
Ksp22I	TGATCA	1 cut(s)	3976
KspAI	GTTAAC	1 cut(s)	4137
LguI	GCTCTTC	5 cut(s)	65, 2579, 3471, 5065, 5831
LmnI	GCTCC	9 cut(s)	522, 718, 730, 802, 1654, 3323, 4610, 4728, 5141
MaeII	ACGT	4 cut(s)	1338, 2545, 5181, 5910
MbiI	CCGCTC	2 cut(s)	110, 1855
MfeI	CAATTG	3 cut(s)	444, 1917, 5846
MflI	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
MhlI	GDGCHC	4 cut(s)	585, 2190, 2428, 4725
MlsI	TGGCCA	3 cut(s)	4338, 4888, 5274
MluNI	TGGCCA	3 cut(s)	4338, 4888, 5274
MmeI	TCCRAC	9 cut(s)	572, 639, 962, 1937, 2802, 3638, 3693, 3943, 4108
Mox20I	TGGCCA	3 cut(s)	4338, 4888, 5274
Mph1103I	ATGCAT	3 cut(s)	226, 1182, 1393
MroXI	GAANNNNTTC	4 cut(s)	1035, 3495, 3720, 4749
MscI	TGGCCA	3 cut(s)	4338, 4888, 5274
MslI	CAYNNNNRTG	3 cut(s)	4649, 4761, 5286
Msp20I	TGGCCA	3 cut(s)	4338, 4888, 5274
MspA1I	CMGCKG	5 cut(s)	1610, 3884, 4366, 5534, 5855
MspCI	CTTAAG	3 cut(s)	466, 2270, 2704
MspI	CCGG	5 cut(s)	261, 833, 5870, 5928, 6000
MunI	CAATTG	3 cut(s)	444, 1917, 5846
Mva1269I	GAATGC	2 cut(s)	2873, 5530
MvaI	CCWGG	9 cut(s)	694, 768, 960, 1107, 1761, 1953, 2148, 4218, 5963
NciI	CCSGG	2 cut(s)	5870, 6001
NcoI	CCATGG	1 cut(s)	5166
NdeI	CATATG	4 cut(s)	2859, 4115, 4758, 5701
NheI	GCTAGC	2 cut(s)	4081, 5471
NmuCI	GTSAC	7 cut(s)	1279, 1452, 1558, 2566, 3399, 5156, 5797
NsiI	ATGCAT	3 cut(s)	226, 1182, 1393
NspI	RCATGY	8 cut(s)	561, 1621, 2264, 3111, 3699, 3763, 4159, 5845
NspV	TTCGAA	1 cut(s)	1600
OliI	CACNNNNGTG	1 cut(s)	5286
PaeI	GCATGC	3 cut(s)	1621, 4159, 5845
PagI	TCATGA	1 cut(s)	4483
PaqCI	CACCTGC	1 cut(s)	4903
PceI	AGGCCT	3 cut(s)	464, 1636, 3407
PciI	ACATGT	3 cut(s)	2260, 3107, 3759
PciSI	GCTCTTC	5 cut(s)	65, 2579, 3471, 5065, 5831
PcsI	WCGNNNNNNNCGW	2 cut(s)	1589, 4613
PctI	GAATGC	2 cut(s)	2873, 5530
PdmI	GAANNNNTTC	4 cut(s)	1035, 3495, 3720, 4749
PflFI	GACNNNGTC	1 cut(s)	4834
PflMI	CCANNNNNTGG	1 cut(s)	4410
PfoI	TCCNGGA	1 cut(s)	2146
PmaCI	CACGTG	1 cut(s)	2546
PmlI	CACGTG	1 cut(s)	2546
Ppu21I	YACGTR	1 cut(s)	2546
PpuMI	RGGWCCY	1 cut(s)	604
PscI	ACATGT	3 cut(s)	2260, 3107, 3759
PshAI	GACNNNNGTC	1 cut(s)	2308
PsiI	TTATAA	2 cut(s)	2172, 4095
Psp124BI	GAGCTC	2 cut(s)	585, 4725
Psp5II	RGGWCCY	1 cut(s)	604
Psp6I	CCWGG	9 cut(s)	692, 766, 958, 1105, 1759, 1951, 2146, 4216, 5961
PspCI	CACGTG	1 cut(s)	2546
PspEI	GGTNACC	1 cut(s)	5156
PspFI	CCCAGC	2 cut(s)	5575, 5861
PspGI	CCWGG	9 cut(s)	692, 766, 958, 1105, 1759, 1951, 2146, 4216, 5961
PspPPI	RGGWCCY	1 cut(s)	604
PstI	CTGCAG	1 cut(s)	790
PstNI	CAGNNNCTG	2 cut(s)	1652, 5858
PsuI	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
PsyI	GACNNNGTC	1 cut(s)	4834
PvuII	CAGCTG	3 cut(s)	1610, 4366, 5534
RsaI	GTAC	8 cut(s)	1248, 2052, 2220, 3527, 3937, 4383, 4699, 5625
RsaNI	GTAC	8 cut(s)	1247, 2051, 2219, 3526, 3936, 4382, 4698, 5624
RseI	CAYNNNNRTG	3 cut(s)	4649, 4761, 5286
SacI	GAGCTC	2 cut(s)	585, 4725
SapI	GCTCTTC	5 cut(s)	65, 2579, 3471, 5065, 5831
ScaI	AGTACT	4 cut(s)	2052, 2220, 3527, 4699
SduI	GDGCHC	4 cut(s)	585, 2190, 2428, 4725
SfcI	CTRYAG	4 cut(s)	786, 2520, 3599, 5175
SfuI	TTCGAA	1 cut(s)	1600
SinI	GGWCC	8 cut(s)	161, 604, 770, 2144, 2375, 3342, 4310, 5008
SmiMI	CAYNNNNRTG	3 cut(s)	4649, 4761, 5286
SpeI	ACTAGT	1 cut(s)	4534
SphI	GCATGC	3 cut(s)	1621, 4159, 5845
SseBI	AGGCCT	3 cut(s)	464, 1636, 3407
SspI	AATATT	1 cut(s)	621
SstI	GAGCTC	2 cut(s)	585, 4725
StuI	AGGCCT	3 cut(s)	464, 1636, 3407
StyI	CCWWGG	3 cut(s)	273, 1443, 5166
TaiI	ACGT	4 cut(s)	1341, 2548, 5184, 5913
TatI	WGTACW	6 cut(s)	2050, 2218, 3525, 4381, 4697, 5623
TauI	GCSGC	6 cut(s)	110, 1858, 3553, 5243, 5531, 5858
TseFI	GTSAC	7 cut(s)	1279, 1452, 1558, 2566, 3399, 5156, 5797
Tsp45I	GTSAC	7 cut(s)	1279, 1452, 1558, 2566, 3399, 5156, 5797
TspGWI	ACGGA	9 cut(s)	112, 140, 891, 1598, 2450, 2513, 3086, 3367, 5820
Tth111I	GACNNNGTC	1 cut(s)	4834
Van91I	CCANNNNNTGG	1 cut(s)	4410
Vha464I	CTTAAG	3 cut(s)	466, 2270, 2704
VneI	GTGCAC	1 cut(s)	2424
VpaK11BI	GGWCC	8 cut(s)	161, 604, 770, 2144, 2375, 3342, 4310, 5008
XagI	CCTNNNNNAGG	2 cut(s)	1447, 3704
XapI	RAATTY	8 cut(s)	155, 473, 2366, 3023, 3120, 3364, 3716, 5603
XbaI	TCTAGA	1 cut(s)	5738
XceI	RCATGY	8 cut(s)	561, 1621, 2264, 3111, 3699, 3763, 4159, 5845
XcmI	CCANNNNNNNNNTGG	2 cut(s)	100, 4763
XmiI	GTMKAC	1 cut(s)	5766
XmnI	GAANNNNTTC	4 cut(s)	1035, 3495, 3720, 4749
ZrmI	AGTACT	4 cut(s)	2052, 2220, 3527, 4699
Zsp2I	ATGCAT	3 cut(s)	226, 1182, 1393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.