Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007772

Physical Location & Seq

Genomic Coordinates Reverse (-)

36139504 .. 36155236

Gene Structure 15733 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1304000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6042 bp

ATGTCGGAGGAGGAGAAATTGCTCAAGGAGGCGAAGAAACTGCCGTGGGACGATCGGCTCTTCCACAAGAACTGGAAGGTGAGGAACGAGGCCAACATTGACTTGGCCGCTCTCTGCGACTCCATCGCCGATCCCAAAGACTCTCGCCTTCGTGAATTTGGTCCGCTTTTTAGGAAGACTCTGGCGGATTCCAATTCGCCAGTGCAAGAGAAGGCGCTCGATGCCTTGATTGCATTTTTACGAGCTGCGGATGCTGATGCCGGAAGATATGCCAAGGAAGTTTGTGATGCCATTGTGGCCAAATGCCTCACTGGTAGGCCGAAGACGGTGGAAAAGGCTCAAGCTGCATTTATGCTTTGGATTGAATTGGAGGCCGTGGACGCTTTCTTGGATGCTATGGAGAAGGCAATAAAAAACAAAGTTTCCAAAGCTGTTGTGCCTGCAATTGATGTCATGTTTCAAGCCTTAAGTGAATTTGGCGCAAAAGTTGTTCCTCCGAAAAGGGTTTTGAAGATGCTCCCCGAACTATTTGACCATCAAGATCAAAATGTTCGTGCATGTTCCAAAGGGCTTACTCTTGAGCTTTGCCGTTGGATAGGAAAAGACCCTGTTAAATCAATATTGTTTGAGAAGATGAGGGATACAATGAAAAAAGAGTTGGAGGCTGAGCTTATAAATGTTACAGGGATAGCCAGGCCATCTCGCAAAATCAGATTGGAGCAAGACAAGGAGCCAGAAAAGGAAGCTGTTTCTGAAGTAGTTGGTCCTAGTCCTTCGGAGGAATCTGCAGCAGATGCTCCTCAGGAAATAGATGAATATGAGCTTGTTGATCCTGTTGATATATTGACTCCTTTGGAGAAGTCTGGATTTTGGGATGGAGTGAAAGCTACAAAGTGGTCAGAACGAAAGGAGGCTGTTGCAGAGTTAACCAAGCTTGCTTCAACTAAAAGAATTGCCCCTGGCGATTTTACAGAAATCTGTCGGACATTAAAGAAGCTTATAACAGATGTGAACATAGCTGTTGCAGTTGAAGCTATTCAAGCTATTGGTAATCTTGCTAAGGGTCTAAGAACTCATTTTTCTGGGAGTTCACGATTTCTATTGCCAGGTTTACTTGAAAAATTGAAAGAAAAAAAACCAACTATGACTGAGGCACTCTCACAAGCTCTTCAAACAATGCATACTGCTGGCTGCTTAAATCTCATTGACATTGTTGAAGATATTAAGACAGCAGTAAAAAATAAAGTACCACTTGTGCGTTCATTGACTTTGAACTGGGTGACATTTTGTATTGAAACAAGCAACAAGGCTGTTGTTTTAAAGTTACACAAGGATTACGTTCCTATATTAATGGAGTGCCTCAACGATGGAACCCCAGACGTGAGGGATGCATCCTTTTCAGCTTTGGCGGCAATAGCTAAGGTAATGGTTGGTATGAGGCCCTTGGAAAGGTCACTTGAGAAACTTGATGATGTCAGAAGAAAGAAGCTCTCTGAAATGATTATGGGTTCTGAAGGTGGTGCATCCACTACTGTGAGCTCAGCCCAAAGCTCTGGTGTGACTGCACCCTCTTTGGAGACTACAGAGAGTTCATTTGTTCGAAGGTCAGCTGCAAGCATGCTTAGTGGGAAGAGGCCTGTTCAGGCAGCTCCTGCAAAACAAAAAGGGGGATCTGTTAAATCGGGTGGCAGTAAGAAAGGAGATGCAACTGTTCAGCCAAAAGCTTCTAAGTTAATTGAAATACCTGAAGATGTTGAGCCAGGTGATATGAGTCTTGAAGAGATTGAAAGCAGATTAGGTTCTCTTATACAAGAAGATACTGTCTCTCAATTAAAGAGTAGTGCATGGAAAGAACGGCTTGAAGCCATATCCTCATTTAAACAGCAAGTCGAAGGTTTACAGGACATTGACCATTCAATTGAGATGTTAGTTCGTTTACTTTGTGCTGTCCCTGGTTGGAGTGAAAAAAATGTTCAGGTTCAGCAACAGGTTATTGAAGTTATTACCTATATGGCGTCCACTGCAAAGAAGTTTCCTAAGAAATGTGTAGTACTTTGTCTTCTGGGTATTAGTGAACGGGTTGCAGATATCAAGACCCGGACTCATGCCATGAAGTGCCTCACTGCTTTTTCTGAAGCCGTAGGTCCAGGATTAATTTTTGAAAGACTTTACAAAATTATGAAAGAGCACAAGAACCCTAAGGTTCTCAGTGAGGGAGTATTGTGGATGGTTTCAGCAGTAGAAGACTTTGGTGTCGCACATGTGAAACTCAAGGATTTGATTGATTTTTGTAAAGAAACTGGACTGCAGTCCAGTGCTGCTGCCACTAGGAACTCTACTATTAAGCTTTTGGGTGCTATACATAAATTTGTTGGTCCAGATATTAAAGGGTTTCTTACTGATGTTAAACCTGCACTACTCAGTGCACTGGACGCAGAGTATGAAAAGAATCCATTTGAGGGTGCTTCTGTGGTTCTGAAGAGAAGTGTTAGGGCAACAGAATCTACTTCATCTGTCTCTGTTGGTGGGTTAGATGGCCTTCCACGTGAAGATATTAGTGGAAAGGTCACCCCTACGCTGCTGAAGAGTTTGGAGAGTCCTGACTGGAAGGTTCGCTTGGAGTCGATAGAAGCTGTCAATAAAATTTTAGAAGAAGCTAATAAGCGCATCCAGCCAACTGGAATGGCGGAGTTGTTTGGTGCCTTAAGGGGGCGCTTATATGATAGCAACAAGAACTTGGTCGCTGCAACCTTAACGGTTGTTGGTAATGTTGCATCTGCAATGGGCGCACAAGTTGAAAAGTTCAGCAAGGGCATTCTGTCGGATGTTTTGAAATGTCTAGGAGACAATAAGAAGCATATGAGAGAATGCACTTTAACTGCTTTAGACTCATGGCTCTCCGCAGTTCATCTTGATAAAATGGTTCCTTATATTACAGCAGCTATATCAGACACCAAGCTTGGTGCAGAAGGGCGGAAAGATCTTTTCGAGTGGTTGACTAGACAGCTTTCTGGGTTAAGTGATTTCTCTGATGCTGCTCATCTTCTGAAACCAGCTTCCTCTGCTTTGACGGATAAATCCTCAGATGTTCGTAAAGCAGCAGAGACATGCGTTTCGGAAATTTTGAGAGTTAGTGGGCCTGAAACGGTTGAGAAGATTTTGAGAGATATTCATGGGCCAACTTTAGCTCTTGTTGAACGGTTGAGGCCTCATGGATCTTTTCAAGAATCTTTTGAGTCGGCCAAAGCAATACCGATGGCCCCAACTTCCAAAAGCATTTCAAAGGCTGGAAAGTCTGCTTCCAATGGAGTTTTGAAGCATGGAGCCAAAGCTACTTCAAGGACCATTCCCACGAAGGGATCAAATTCAATGCTGTCTGTCCAAGATATAGCTGTCCAGTCACAGGCCTTGATAAATGTTAAAGATTCAATTAAGGAGGATAGAGAGCGACTGGTAGCTAAAAAGTTTAAGTTTGAAGAGCCACGAATAGAACAGATTCAAGATCTTGAGAATGATATGACGAAGTACTTTAGAGAGGATGTGCATAGGCGGCTTCTTTCTACAGATTTTAAGAAGCAAGTTGATGGGCTTGAGATACTACAGAAGGCGCTTCCGACCATTAAAAAGGAAATTATTGAAGTATTGGATATACTGCTGAGGTGGTTTGTTTTACAATTTTGTAAATCGAACACAACGTGCCTGTTAAAGGTGCTGGAATTTCTGCCAGACCTTTTTGATACATTTAGGGATGAGGCGTACATTTTGACTGAATCAGAAGCGGCCATATTTTTTCCATGCTTGATAGAGAAGCTTGGGCATAACATTGAGAAAGTACGAGAAAAAATGCGGGAGCTGACCAAACAAATTGTGCAGGCATATACTGCTGCAAAAAGCTTCCCTTATATTTTGGAGGGATTACGTTCTAAGAACAACCGTACTCGAATTGAGTGTGCTGATCTTGTTGGATATTTGATTGATCACCACGTAGCTGAGATTAGTGGACAACTAAAGTCTTTGCAAATTGTTGCGAGCTTGACAGCAGAAAGAGATGGTGAAATTAGGAAAGCTGCTTTAAATACGCTAGCTACTGGTTATAAGATTCTTGGTGAGGACATATGGAGATACGTCGGAAAGTTAACAGATGCTCAGAAAAGCATGCTAGATGATCGATTTAAGTGGAAGGTTCGAGAAATGGAGAAAAGGAAGGAAGGAAAGCCTGGCGAAGCTAGGGCTTCTCTGAGGCGTTCTGTCAGGGAAATTGGGCCTGATGTAGCAGAGCAAAGTGGGGAGGTTGCTCGATCTGTTTCTGGTCCAATGCTTTCTAGGAGAAACTATGGCCATCCTGAGCCTCACTTGGAGAGGCAACTGATGCCACCCCATGTACTCACTGGTGCCAATGGCCCAACAGACTGGAATGAAGCTTTGGATATCATTTCTTTTGGTTCTCCTGAACAGTCAGTTGAAGGAATGAAAGTAGTGTGTCATGAGTTGGCACAGGCGACTAATGATCCAGAAGGCAGTGCTATGGATGAACTAGTGCGTGATGCAGATAGACTGGTTTCTTGCTTGGCAAATAAGGTAGCAAAGACTTTTGACTTCAGTTTGACAGGAGCTTCATCTAGGTCTTGTAAATATGTCCTGAACACTCTTATGCAGACATTTCAAAATAAAGGACTTGCTTATGCTGTCAAGGAAAGTACTCTTGACAGTCTAATCACTGAGCTCCTTCTGTGGCTTTTGGATGAAAGGGTTCCCCATATGGACGATGGCAGCCAACTCCTGAAAGCCTTGAATGTTTTGATGCTAAAGATTCTGGATAATGCAGATCGGACTTCGTCCTTTGTTGTTCTCATCAACCTCTTACATCCTTTAGATCCCTCAAGATGGCCATCACCTGCATCCAATGAATCATTTACTTCCAGAAATCAGAAGTTCTCCGATCTGGTTGTCAAATGTTTAATAAAACTTACAAAGGTTCTTCAAAGCACAATATATGATGTCGAATTGGACCGTATCCTTCAAAGCATCCATTTATACCTACAAGACTTAGGGATGGAAGAGATTAGGAGAAGAGCTGGTGCTGATGACAAACCACTGCGTATGGTGAAGACCATTCTGCATGAGCTTGTGAAGCTTCGTGGAGCTGCAATTAAAGGTCACCTTTCCATGGTTCCTATAGACGTAAAACCGCAGCCCATCATTCTTGCCTACATTGATCTTAATCTTGAGACTTTAGCTGCGGCAAGGATGCTGACATCAACTGGGTCTGGGGGCCAAACTCATTGGGGTGATTCAGCAGCCAACAATCCTTCATCTGCCACACATTCTGCTGATGCACAGTTAAAGCAAGAACTTGCTGCCATATTCAAGAAGATTGGCGACAAGCAAACTTGCAGCATTGGTCTATATGAACTATATCGCATAACCCAACTGTACCCAAGAGTTGATATATTCTCTCAACTCCAAAATGCTAGCGAGGCTTTCCGGACATATATTAGAGATGGTTTGGCGCAGATGGAGAAGAATGCCGCAGCTGGAAGGACGCCTTCAAGTTTGCCAATGCCAACTCCTCCCCCAGCTACTCTAAATGCTTCTTCACCTGAATTTGCACCCCTGTCCCCTGTACACACGAACTCCTTGGTTGATTCGAAATCATTAAATGTGAAATCCGAACCAACTAGCTTTAATCTACCGCCATCATATACTGAAGAATACAGGCTGAATAATGCCAGAGGTCTCACCGAAAACTCATTGGTAGACCAAAGGAATGAGAGATATATTGGAGTGACCAACGGAACCTTAGATGCAATTAGAGAGAGAATGAAGAGCATGCAGTTGGCCGCAGCTGCTGGGAACCCGGATCAAGAAACCAGGCCATTAATGTATGCGAATGACAACATAAACCAGAGCCTCTCTGGTCAGATTCCTCGTGCATCAGAGAACCCACTCCAGAGTGCGGTTCACCCCATGGATGAGAGGGCATTATCCGGACTTCAAGCCCGTATGGAGAGACTAAAAAGTGGGACAATCGAACCCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000775 GO:0000776 GO:0000910 GO:0000911 GO:0000919 GO:0000922 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005911 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007163 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009574 GO:0009832 GO:0009920 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0030054 GO:0030863 GO:0030951 GO:0030952 GO:0030981 GO:0031109 GO:0032506 GO:0032991 GO:0034622 GO:0042546 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046785 GO:0048285 GO:0051010 GO:0051258 GO:0051301 GO:0055044 GO:0061640 GO:0065003 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0097435 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT

Protein Analysis

2014

Amino Acids

222.31

Weight (kDa)

7.92

Isoelectric Point (pI)

45.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XMAP215_CLASP_TOG	PF21041	11 - 223	4.4e-61	XMAP215/Dis1/CLASP, TOG domain
XMAP215_CLASP_TOG	PF21041	297 - 499	2.7e-07	XMAP215/Dis1/CLASP, TOG domain
CLASP_N	PF12348	298 - 472	3.5e-09	CLASP N terminal
XMAP215_CLASP_TOG	PF21041	610 - 813	3.2e-12	XMAP215/Dis1/CLASP, TOG domain
XMAP215_CLASP_TOG	PF21041	1184 - 1391	6.5e-06	XMAP215/Dis1/CLASP, TOG domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0007772)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35630 AT2G35630
fragaria_vesca	FvH4_7g33390 FvH4_7g33390 FvH4_7g33390
malus_domestica	MD01G1232200.v1.1 MD07G1304000.v1.1
prunus_persica	Prupe.1G083500_v2.0.a1 Prupe.1G083500_v2.0.a1 Prupe.1G083500_v2.0.a1 Prupe.1G083500_v2.0.a1
pyrus_communis	pycom01g24060
rosa_chinensis	RchiOBHm_Chr1g0383361
rosa_laevigata	RLG00000026109
rosa_multiflora	Rmu_sc0007173.1_g000012
rosa_roxburghii	Rroxscaffold_4G00277360
rosa_rugosa	Rorug01G0440800 Rorug01G0440800 Rorug01G0440900
rosa_samantha	Rh1AG466800 Rh1BG424100 Rh1CG437200 Rh1DG456800
rosa_wichuraiana	Rw1G040580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	674, 1001, 4095
AarI	CACCTGC	1 cut(s)	4903
AasI	GACNNNNNNGTC	1 cut(s)	2252
Acc36I	ACCTGC	2 cut(s)	2419, 4903
AccB1I	GGYRCC	2 cut(s)	2699, 4391
AccBSI	CCGCTC	1 cut(s)	110
AccI	GTMKAC	1 cut(s)	5757
AccIII	TCCGGA	2 cut(s)	5484, 5987
AclWI	GGATC	8 cut(s)	125, 824, 1678, 3223, 3367, 4502, 4868, 5868
AcoI	YGGCCR	7 cut(s)	105, 297, 3240, 3780, 4336, 4886, 5838
AcsI	RAATTY	8 cut(s)	155, 473, 2366, 2643, 3120, 3364, 3716, 5603
AcuI	CTGAAG	8 cut(s)	774, 1533, 1767, 2154, 2498, 2603, 4582, 5727
AcvI	CACGTG	1 cut(s)	2546
AcyI	GRCGYC	2 cut(s)	2015, 5543
AdeI	CACNNNGTG	1 cut(s)	3696
AfiI	CCNNNNNNNGG	9 cut(s)	1449, 2459, 2709, 3356, 3403, 3706, 4884, 5167, 5956
AflII	CTTAAG	2 cut(s)	466, 2704
AflIII	ACRYGT	1 cut(s)	2260
AhlI	ACTAGT	1 cut(s)	4534
AjiI	CACGTC	1 cut(s)	1381
AjnI	CCWGG	8 cut(s)	692, 958, 1105, 1759, 1951, 2146, 4216, 5870
AjuI	GAANNNNNNNTTGG	6 cut(s)	641, 673, 2600, 2632, 4406, 4438
AleI	CACNNNNGTG	1 cut(s)	1532
Alw21I	GWGCWC	4 cut(s)	1541, 2190, 2428, 4725
Alw26I	GTCTC	8 cut(s)	1571, 1828, 2521, 2838, 3098, 5222, 5741, 6004
Alw44I	GTGCAC	1 cut(s)	2424
AlwI	GGATC	8 cut(s)	125, 824, 1678, 3223, 3367, 4502, 4868, 5868
AlwNI	CAGNNNCTG	2 cut(s)	1652, 5849
Aor13HI	TCCGGA	2 cut(s)	5484, 5987
ApaLI	GTGCAC	1 cut(s)	2424
ApoI	RAATTY	8 cut(s)	155, 473, 2366, 2643, 3120, 3364, 3716, 5603
ArsI	GACNNNNNNTTYG	2 cut(s)	3097, 3129
AseI	ATTAAT	3 cut(s)	1349, 2153, 5879
Asp700I	GAANNNNTTC	3 cut(s)	1035, 3495, 4749
AspLEI	GCGC	7 cut(s)	217, 482, 2667, 2715, 2789, 3610, 5512
AsuC2I	CCSGG	2 cut(s)	2098, 5858
AsuII	TTCGAA	2 cut(s)	1600, 5648
AsuNHI	GCTAGC	2 cut(s)	4081, 5471
AvaII	GGWCC	7 cut(s)	161, 764, 2144, 2375, 3342, 4310, 5008
AxyI	CCTNAGG	2 cut(s)	801, 2199
BaeGI	GKGCMC	1 cut(s)	2428
BalI	TGGCCA	3 cut(s)	299, 4338, 4888
BanI	GGYRCC	2 cut(s)	2699, 4391
BanII	GRGCYC	2 cut(s)	1541, 4725
BarI	GAAGNNNNNNTAC	2 cut(s)	2043, 2075
BauI	CACGAG	1 cut(s)	5928
BbrPI	CACGTG	1 cut(s)	2546
BbsI	GAAGAC	5 cut(s)	182, 329, 2051, 2250, 5114
Bbv12I	GWGCWC	4 cut(s)	1541, 2190, 2428, 4725
BbvCI	CCTCAGC	1 cut(s)	3656
BceAI	ACGGC	5 cut(s)	28, 359, 573, 1871, 2123
BcgI	CGANNNNNNTGC	2 cut(s)	22, 56
BciT130I	CCWGG	8 cut(s)	694, 960, 1107, 1761, 1953, 2148, 4218, 5872
BciVI	GTATCC	2 cut(s)	634, 5024
BclI	TGATCA	1 cut(s)	3976
BcnI	CCSGG	2 cut(s)	2098, 5858
BcoDI	GTCTC	8 cut(s)	1571, 1828, 2521, 2838, 3098, 5222, 5741, 6004
BcuI	ACTAGT	1 cut(s)	4534
BfmI	CTRYAG	6 cut(s)	786, 1581, 2306, 3561, 3599, 5175
BfoI	RGCGCY	3 cut(s)	218, 2716, 3611
BfrI	CTTAAG	2 cut(s)	466, 2704
BfuAI	ACCTGC	2 cut(s)	2419, 4903
BfuI	GTATCC	2 cut(s)	634, 5024
BglI	GCCNNNNNGGC	1 cut(s)	296
BglII	AGATCT	2 cut(s)	2980, 3502
BlpI	GCTNAGC	2 cut(s)	666, 1540
BmcAI	AGTACT	3 cut(s)	2052, 3527, 4699
Bme18I	GGWCC	7 cut(s)	161, 764, 2144, 2375, 3342, 4310, 5008
BmgBI	CACGTC	1 cut(s)	1381
BmrI	ACTGGG	2 cut(s)	1285, 5271
BmtI	GCTAGC	2 cut(s)	4085, 5475
BmuI	ACTGGG	2 cut(s)	1285, 5271
BoxI	GACNNNNGTC	1 cut(s)	2308
BpiI	GAAGAC	5 cut(s)	182, 329, 2051, 2250, 5114
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
BpmI	CTGGAG	1 cut(s)	5933
Bpu10I	CCTNAGC	4 cut(s)	1059, 1419, 3656, 4344
Bpu1102I	GCTNAGC	2 cut(s)	666, 1540
Bpu14I	TTCGAA	2 cut(s)	1600, 5648
BpuEI	CTTGAG	9 cut(s)	8, 324, 599, 1478, 2255, 3527, 3611, 4864, 5246
BpuMI	CCSGG	2 cut(s)	2098, 5858
Bsa29I	ATCGAT	1 cut(s)	4168
BsaAI	YACGTR	2 cut(s)	2546, 3985
BsaBI	GATNNNNATC	2 cut(s)	4888, 5220
BsaHI	GRCGYC	2 cut(s)	2015, 5543
BsaI	GGTCTC	1 cut(s)	5741
BsaJI	CCNNGG	9 cut(s)	44, 273, 375, 958, 1443, 1951, 5166, 5637, 5967
BsaWI	WCCGGW	2 cut(s)	5484, 5987
BsaXI	ACNNNNNCTCC	2 cut(s)	5618, 5648
Bsc4I	CCNNNNNNNGG	9 cut(s)	1449, 2459, 2709, 3356, 3403, 3706, 4884, 5167, 5956
Bse21I	CCTNAGG	2 cut(s)	801, 2199
Bse3DI	GCAATG	1 cut(s)	2787
Bse8I	GATNNNNATC	2 cut(s)	4888, 5220
BseAI	TCCGGA	2 cut(s)	5484, 5987
BseBI	CCWGG	8 cut(s)	694, 960, 1107, 1761, 1953, 2148, 4218, 5872
BseCI	ATCGAT	1 cut(s)	4168
BseDI	CCNNGG	9 cut(s)	44, 273, 375, 958, 1443, 1951, 5166, 5637, 5967
BseJI	GATNNNNATC	2 cut(s)	4888, 5220
BseLI	CCNNNNNNNGG	9 cut(s)	1449, 2459, 2709, 3356, 3403, 3706, 4884, 5167, 5956
BseMI	GCAATG	1 cut(s)	2787
BseRI	GAGGAG	4 cut(s)	23, 26, 789, 5559
BseSI	GKGCMC	1 cut(s)	2428
BseYI	CCCAGC	2 cut(s)	5575, 5849
BsgI	GTGCAG	4 cut(s)	1548, 2397, 2985, 3890
Bsh1285I	CGRYCG	1 cut(s)	55
BshNI	GGYRCC	2 cut(s)	2699, 4391
BshVI	ATCGAT	1 cut(s)	4168
BsiEI	CGRYCG	1 cut(s)	55
BsiHKAI	GWGCWC	4 cut(s)	1541, 2190, 2428, 4725
BsiSI	CCGG	5 cut(s)	261, 2098, 5485, 5858, 5988
BslFI	GGGAC	4 cut(s)	62, 1934, 5602, 6037
BslI	CCNNNNNNNGG	9 cut(s)	1449, 2459, 2709, 3356, 3403, 3706, 4884, 5167, 5956
BsmAI	GTCTC	8 cut(s)	1571, 1828, 2521, 2838, 3098, 5222, 5741, 6004
BsmFI	GGGAC	4 cut(s)	62, 1934, 5602, 6037
BsmI	GAATGC	3 cut(s)	2814, 2873, 5530
Bso31I	GGTCTC	1 cut(s)	5741
Bsp119I	TTCGAA	2 cut(s)	1600, 5648
Bsp1286I	GDGCHC	4 cut(s)	1541, 2190, 2428, 4725
Bsp13I	TCCGGA	2 cut(s)	5484, 5987
Bsp1407I	TGTACA	1 cut(s)	5623
Bsp1720I	GCTNAGC	2 cut(s)	666, 1540
Bsp19I	CCATGG	2 cut(s)	5166, 5967
BspDI	ATCGAT	1 cut(s)	4168
BspEI	TCCGGA	2 cut(s)	5484, 5987
BspHI	TCATGA	1 cut(s)	4483
BspMAI	CTGCAG	2 cut(s)	790, 2310
BspMI	ACCTGC	2 cut(s)	2419, 4903
BspOI	GCTAGC	2 cut(s)	4085, 5475
BspPI	GGATC	8 cut(s)	125, 824, 1678, 3223, 3367, 4502, 4868, 5868
BspQI	GCTCTTC	5 cut(s)	65, 1173, 3471, 5065, 5819
BspT104I	TTCGAA	2 cut(s)	1600, 5648
BspT107I	GGYRCC	2 cut(s)	2699, 4391
BspTI	CTTAAG	2 cut(s)	466, 2704
BspTNI	GGTCTC	1 cut(s)	5741
BsrBI	CCGCTC	1 cut(s)	110
BsrDI	GCAATG	1 cut(s)	2787
BsrGI	TGTACA	1 cut(s)	5623
BssECI	CCNNGG	9 cut(s)	44, 273, 375, 958, 1443, 1951, 5166, 5637, 5967
BssNI	GRCGYC	2 cut(s)	2015, 5543
BssSI	CACGAG	1 cut(s)	5928
BssT1I	CCWWGG	5 cut(s)	273, 1443, 5166, 5637, 5967
Bst2BI	CACGAG	1 cut(s)	5928
Bst2UI	CCWGG	8 cut(s)	694, 960, 1107, 1761, 1953, 2148, 4218, 5872
BstACI	GRCGYC	2 cut(s)	2015, 5543
BstAFI	CTTAAG	2 cut(s)	466, 2704
BstAPI	GCANNNNNTGC	4 cut(s)	794, 1652, 3881, 4369
BstAUI	TGTACA	1 cut(s)	5623
BstBAI	YACGTR	2 cut(s)	2546, 3985
BstBI	TTCGAA	2 cut(s)	1600, 5648
BstDSI	CCRYGG	4 cut(s)	44, 375, 5166, 5967
BstEII	GGTNACC	2 cut(s)	2566, 5156
BstENI	CCTNNNNNAGG	2 cut(s)	1447, 3704
BstH2I	RGCGCY	3 cut(s)	218, 2716, 3611
BstHHI	GCGC	7 cut(s)	217, 482, 2667, 2715, 2789, 3610, 5512
BstMAI	GTCTC	8 cut(s)	1571, 1828, 2521, 2838, 3098, 5222, 5741, 6004
BstMCI	CGRYCG	1 cut(s)	55
BstNI	CCWGG	8 cut(s)	694, 960, 1107, 1761, 1953, 2148, 4218, 5872
BstNSI	RCATGY	6 cut(s)	561, 1621, 2264, 3111, 4159, 5833
BstPAI	GACNNNNGTC	1 cut(s)	2308
BstPI	GGTNACC	2 cut(s)	2566, 5156
BstSFI	CTRYAG	6 cut(s)	786, 1581, 2306, 3561, 3599, 5175
BstSLI	GKGCMC	1 cut(s)	2428
BstV2I	GAAGAC	5 cut(s)	182, 329, 2051, 2250, 5114
BstX2I	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
BstXI	CCANNNNNNTGG	3 cut(s)	1553, 2678, 4410
BstYI	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
Bsu15I	ATCGAT	1 cut(s)	4168
Bsu36I	CCTNAGG	2 cut(s)	801, 2199
BsuI	GTATCC	2 cut(s)	634, 5024
BsuTUI	ATCGAT	1 cut(s)	4168
BtgI	CCRYGG	4 cut(s)	44, 375, 5166, 5967
BtgZI	GCGATG	1 cut(s)	109
BtrI	CACGTC	1 cut(s)	1381
BtsI	GCAGTG	4 cut(s)	2019, 2121, 4525, 5093
BveI	ACCTGC	2 cut(s)	2419, 4903
CaiI	CAGNNNCTG	2 cut(s)	1652, 5849
CciI	TCATGA	1 cut(s)	4483
CfoI	GCGC	7 cut(s)	217, 482, 2667, 2715, 2789, 3610, 5512
ClaI	ATCGAT	1 cut(s)	4168
CseI	GACGC	4 cut(s)	389, 2004, 2441, 5551
DraI	TTTAAA	3 cut(s)	1320, 1879, 4074
DraIII	CACNNNGTG	1 cut(s)	3696
DrdI	GACNNNNNNGTC	1 cut(s)	2252
DseDI	GACNNNNNNGTC	1 cut(s)	2252
EaeI	YGGCCR	7 cut(s)	105, 297, 3240, 3780, 4336, 4886, 5838
EciI	GGCGGA	3 cut(s)	200, 2702, 2989
Ecl136II	GAGCTC	2 cut(s)	1539, 4723
Eco130I	CCWWGG	5 cut(s)	273, 1443, 5166, 5637, 5967
Eco147I	AGGCCT	3 cut(s)	1636, 3208, 3407
Eco24I	GRGCYC	2 cut(s)	1541, 4725
Eco31I	GGTCTC	1 cut(s)	5741
Eco32I	GATATC	2 cut(s)	2089, 4429
Eco47I	GGWCC	7 cut(s)	161, 764, 2144, 2375, 3342, 4310, 5008
Eco53kI	GAGCTC	2 cut(s)	1539, 4723
Eco57I	CTGAAG	8 cut(s)	774, 1533, 1767, 2154, 2498, 2603, 4582, 5727
Eco72I	CACGTG	1 cut(s)	2546
Eco81I	CCTNAGG	2 cut(s)	801, 2199
Eco91I	GGTNACC	2 cut(s)	2566, 5156
EcoICRI	GAGCTC	2 cut(s)	1539, 4723
EcoNI	CCTNNNNNAGG	2 cut(s)	1447, 3704
EcoO109I	RGGNCCY	1 cut(s)	1440
EcoO65I	GGTNACC	2 cut(s)	2566, 5156
EcoRII	CCWGG	8 cut(s)	692, 958, 1105, 1759, 1951, 2146, 4216, 5870
EcoRV	GATATC	2 cut(s)	2089, 4429
EcoT14I	CCWWGG	5 cut(s)	273, 1443, 5166, 5637, 5967
EcoT22I	ATGCAT	2 cut(s)	1182, 1393
EcoT38I	GRGCYC	2 cut(s)	1541, 4725
ErhI	CCWWGG	5 cut(s)	273, 1443, 5166, 5637, 5967
FaqI	GGGAC	4 cut(s)	62, 1934, 5602, 6037
FauI	CCCGC	1 cut(s)	3840
FauNDI	CATATG	3 cut(s)	2859, 4115, 4758
FbaI	TGATCA	1 cut(s)	3976
FblI	GTMKAC	1 cut(s)	5757
FriOI	GRGCYC	2 cut(s)	1541, 4725
GlaI	GCGC	7 cut(s)	216, 481, 2666, 2714, 2788, 3609, 5511
GsaI	CCCAGC	2 cut(s)	5579, 5853
GsuI	CTGGAG	1 cut(s)	5933
HaeII	RGCGCY	3 cut(s)	218, 2716, 3611
HapII	CCGG	5 cut(s)	261, 2098, 5485, 5858, 5988
HgaI	GACGC	4 cut(s)	389, 2004, 2441, 5551
HhaI	GCGC	7 cut(s)	217, 482, 2667, 2715, 2789, 3610, 5512
Hin1I	GRCGYC	2 cut(s)	2015, 5543
Hin6I	GCGC	7 cut(s)	215, 480, 2665, 2713, 2787, 3608, 5510
HinP1I	GCGC	7 cut(s)	215, 480, 2665, 2713, 2787, 3608, 5510
HincII	GTYRAC	3 cut(s)	927, 2997, 4137
HindII	GTYRAC	3 cut(s)	927, 2997, 4137
HindIII	AAGCTT	9 cut(s)	932, 995, 1722, 2345, 2957, 3809, 3892, 4419, 5132
HpaI	GTTAAC	2 cut(s)	927, 4137
HpaII	CCGG	5 cut(s)	261, 2098, 5485, 5858, 5988
Hpy99I	CGWCG	1 cut(s)	4130
HpyCH4IV	ACGT	8 cut(s)	1338, 1380, 2545, 3695, 3919, 3984, 4125, 5181
HpySE526I	ACGT	8 cut(s)	1338, 1380, 2545, 3695, 3919, 3984, 4125, 5181
Hsp92I	GRCGYC	2 cut(s)	2015, 5543
HspAI	GCGC	7 cut(s)	215, 480, 2665, 2713, 2787, 3608, 5510
Kpn2I	TCCGGA	2 cut(s)	5484, 5987
Ksp22I	TGATCA	1 cut(s)	3976
KspAI	GTTAAC	2 cut(s)	927, 4137
LguI	GCTCTTC	5 cut(s)	65, 1173, 3471, 5065, 5819
MaeII	ACGT	8 cut(s)	1338, 1380, 2545, 3695, 3919, 3984, 4125, 5181
MaeIII	GTNAC	9 cut(s)	679, 1279, 1323, 1452, 1558, 2566, 3399, 5156, 5785
MbiI	CCGCTC	1 cut(s)	110
MfeI	CAATTG	2 cut(s)	444, 1917
MflI	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
MhlI	GDGCHC	4 cut(s)	1541, 2190, 2428, 4725
MlsI	TGGCCA	3 cut(s)	299, 4338, 4888
MluNI	TGGCCA	3 cut(s)	299, 4338, 4888
MmeI	TCCRAC	8 cut(s)	572, 639, 962, 1937, 2802, 3638, 3943, 4108
Mox20I	TGGCCA	3 cut(s)	299, 4338, 4888
Mph1103I	ATGCAT	2 cut(s)	1182, 1393
MroI	TCCGGA	2 cut(s)	5484, 5987
MroXI	GAANNNNTTC	3 cut(s)	1035, 3495, 4749
MscI	TGGCCA	3 cut(s)	299, 4338, 4888
MslI	CAYNNNNRTG	3 cut(s)	1532, 4649, 5286
Msp20I	TGGCCA	3 cut(s)	299, 4338, 4888
MspA1I	CMGCKG	3 cut(s)	1610, 5534, 5846
MspCI	CTTAAG	2 cut(s)	466, 2704
MspI	CCGG	5 cut(s)	261, 2098, 5485, 5858, 5988
MunI	CAATTG	2 cut(s)	444, 1917
Mva1269I	GAATGC	3 cut(s)	2814, 2873, 5530
MvaI	CCWGG	8 cut(s)	694, 960, 1107, 1761, 1953, 2148, 4218, 5872
NciI	CCSGG	2 cut(s)	2098, 5858
NcoI	CCATGG	2 cut(s)	5166, 5967
NdeI	CATATG	3 cut(s)	2859, 4115, 4758
NheI	GCTAGC	2 cut(s)	4081, 5471
NmuCI	GTSAC	7 cut(s)	1279, 1452, 1558, 2566, 3399, 5156, 5785
NsiI	ATGCAT	2 cut(s)	1182, 1393
NspI	RCATGY	6 cut(s)	561, 1621, 2264, 3111, 4159, 5833
NspV	TTCGAA	2 cut(s)	1600, 5648
OliI	CACNNNNGTG	1 cut(s)	1532
PaeI	GCATGC	3 cut(s)	1621, 4159, 5833
PagI	TCATGA	1 cut(s)	4483
PaqCI	CACCTGC	1 cut(s)	4903
PceI	AGGCCT	3 cut(s)	1636, 3208, 3407
PciI	ACATGT	1 cut(s)	2260
PciSI	GCTCTTC	5 cut(s)	65, 1173, 3471, 5065, 5819
PcsI	WCGNNNNNNNCGW	1 cut(s)	3692
PctI	GAATGC	3 cut(s)	2814, 2873, 5530
PdmI	GAANNNNTTC	3 cut(s)	1035, 3495, 4749
PflFI	GACNNNGTC	1 cut(s)	4834
PfoI	TCCNGGA	1 cut(s)	2146
Ple19I	CGATCG	1 cut(s)	55
PmaCI	CACGTG	1 cut(s)	2546
PmlI	CACGTG	1 cut(s)	2546
Ppu21I	YACGTR	2 cut(s)	2546, 3985
PscI	ACATGT	1 cut(s)	2260
PshAI	GACNNNNGTC	1 cut(s)	2308
PshBI	ATTAAT	3 cut(s)	1349, 2153, 5879
PsiI	TTATAA	3 cut(s)	674, 1001, 4095
Psp124BI	GAGCTC	2 cut(s)	1541, 4725
Psp6I	CCWGG	8 cut(s)	692, 958, 1105, 1759, 1951, 2146, 4216, 5870
PspCI	CACGTG	1 cut(s)	2546
PspEI	GGTNACC	2 cut(s)	2566, 5156
PspFI	CCCAGC	2 cut(s)	5575, 5849
PspGI	CCWGG	8 cut(s)	692, 958, 1105, 1759, 1951, 2146, 4216, 5870
PstI	CTGCAG	2 cut(s)	790, 2310
PstNI	CAGNNNCTG	2 cut(s)	1652, 5849
PsuI	RGATCY	5 cut(s)	1670, 2980, 3215, 3502, 4873
PsyI	GACNNNGTC	1 cut(s)	4834
PvuI	CGATCG	1 cut(s)	55
PvuII	CAGCTG	3 cut(s)	1610, 5534, 5846
RseI	CAYNNNNRTG	3 cut(s)	1532, 4649, 5286
SacI	GAGCTC	2 cut(s)	1541, 4725
SapI	GCTCTTC	5 cut(s)	65, 1173, 3471, 5065, 5819
ScaI	AGTACT	3 cut(s)	2052, 3527, 4699
SduI	GDGCHC	4 cut(s)	1541, 2190, 2428, 4725
SfcI	CTRYAG	6 cut(s)	786, 1581, 2306, 3561, 3599, 5175
SfuI	TTCGAA	2 cut(s)	1600, 5648
SinI	GGWCC	7 cut(s)	161, 764, 2144, 2375, 3342, 4310, 5008
SmiMI	CAYNNNNRTG	3 cut(s)	1532, 4649, 5286
SpeI	ACTAGT	1 cut(s)	4534
SphI	GCATGC	3 cut(s)	1621, 4159, 5833
SseBI	AGGCCT	3 cut(s)	1636, 3208, 3407
SspI	AATATT	1 cut(s)	621
SstI	GAGCTC	2 cut(s)	1541, 4725
StuI	AGGCCT	3 cut(s)	1636, 3208, 3407
StyI	CCWWGG	5 cut(s)	273, 1443, 5166, 5637, 5967
TaiI	ACGT	8 cut(s)	1341, 1383, 2548, 3698, 3922, 3987, 4128, 5184
TatI	WGTACW	5 cut(s)	2050, 3525, 4381, 4697, 5623
TauI	GCSGC	7 cut(s)	110, 1412, 3553, 3782, 5243, 5531, 5843
TseFI	GTSAC	7 cut(s)	1279, 1452, 1558, 2566, 3399, 5156, 5785
Tsp45I	GTSAC	7 cut(s)	1279, 1452, 1558, 2566, 3399, 5156, 5785
TspGWI	ACGGA	2 cut(s)	3086, 5808
Tth111I	GACNNNGTC	1 cut(s)	4834
Vha464I	CTTAAG	2 cut(s)	466, 2704
VneI	GTGCAC	1 cut(s)	2424
VpaK11BI	GGWCC	7 cut(s)	161, 764, 2144, 2375, 3342, 4310, 5008
VspI	ATTAAT	3 cut(s)	1349, 2153, 5879
XagI	CCTNNNNNAGG	2 cut(s)	1447, 3704
XapI	RAATTY	8 cut(s)	155, 473, 2366, 2643, 3120, 3364, 3716, 5603
XceI	RCATGY	6 cut(s)	561, 1621, 2264, 3111, 4159, 5833
XcmI	CCANNNNNNNNNTGG	4 cut(s)	100, 4385, 4763, 5912
XmiI	GTMKAC	1 cut(s)	5757
XmnI	GAANNNNTTC	3 cut(s)	1035, 3495, 4749
ZrmI	AGTACT	3 cut(s)	2052, 3527, 4699
Zsp2I	ATGCAT	2 cut(s)	1182, 1393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.