Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004084

Physical Location & Seq

Genomic Coordinates Reverse (-)

32440166 .. 32441287

Gene Structure 1122 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1237400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGGCGCCAAGCACAAACTCACTCGTATCACAATCAGAAGCAGCACCGGTCCGTCGCTACCGCATCGGGTATGCCTTCGCACCCAAAAAAGAGCAGACCTTCATCCAGCCCTCTCTCCTCCACCATGCCTCTCGTTACGGCATCGATTTCGTCCCCATTTCAATAATCGATTCCACCGCTACCGCCAAGACCCTGATCGAGCAAGGCCCCTTCGATTGCATCATCCACAAGCTCTACGGCCCCCACTGGAACCAACAGCTCAAGGAATACTCGTTAAAGTATCCACAAACGCTCATTCTTGACCCGCCCGAGTCCATCGAGCGCCTCCACAATCGGGTCTCCATGCTCGACGTCGTCACCGCCTTTGAATCGAAGCCGCATCAGTCAGGCGACAGATTCCAATCGAAGATCTCGGTGCCCAAACAGGTGCTGGTTGACCATTACCCGGAAGGCGAAGAAGCAACTAATTTGTTGGCGGGGTTGGAGTTTCCGGTGATTGCAAAGCCGTTGTTGGCGAATGGCAGCGCCAAGTCGCACGAGATGTGTTTGATTTTTAACCCCAAGGGGTTGCGCAGTCTTCTGGCCGATACTGATAGTCCCATTTTGCTGCAACAGTTTGTGAACCACGGCGGGGTGGTGTTCAAGGGTTACTCAATCGGAGAGCACGTCCAGTGCGTCAAGCGGAGGTCACTGCCGGACATTTCGGAGGAGCAGGTGACCGCATTGGAAGGGGCAGTGCTTCCTTTTTCCCAGATATCAAATTCTGCGAATGACGAGCATAATCTGGCTCATGATCCTCACGAAATGCCTCCGCTGGAGTTTGTTGAGGAGGTGGCGAAGGGGATACGCGTGGGCCTCAAACTCAACTTTTTCAACTTTGACGTGATAAGGGATTCGAAGGACCCCAGCAGCTATTTTGTGATCGACATCAATTACTTTCCTGGGTATGCCAAATTGCCTAACTACGAGCATGCCTTGACTGACTTTTTGCTGAATCTGCTTGCAACAAGTAGTCATCCCCAAAGCCAAAACCAAAAGGAGCTCCTGCAGCTCAAGGAAGAAGAAGCTGCCACAAGTTTGGAGTCCGATGTCAAACAACACAGGGGTCATAAGGAGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006020 GO:0006066 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010264 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0019637 GO:0019751 GO:0032958 GO:0033517 GO:0043647 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046173 GO:0046835 GO:0051766 GO:0052725 GO:0052726 GO:0052746 GO:0071704 GO:0090407 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Ins134_P3_kin

Protein Analysis

374

Amino Acids

41.83

Weight (kDa)

5.78

Isoelectric Point (pI)

51.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ins134_P3_kin_N	PF17927	21 - 106	2e-25	Inositol 1,3,4-trisphosphate 5/6-kinase pre-ATP-grasp domain
Ins134_P3_kin	PF05770	135 - 331	1.3e-65	Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004084)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16760
fragaria_vesca	FvH4_5g22490 FvH4_7g34131
malus_domestica	MD01G1237400.v1.1 MD03G1144600.v1.1 MD06G1037200.v1.1 MD07G1309900.v1.1 MD16G1271300.v1.1
prunus_persica	Prupe.2G327800_v2.0.a1 Prupe.5G031800_v2.0.a1
pyrus_communis	pycom06g03060 pycom07g28190 pycom16g24080
rosa_chinensis	RchiOBHm_Chr1g0384451 RchiOBHm_Chr7g0210271
rosa_laevigata	RLG00000003053 RLG00000026039
rosa_multiflora	Rmu_co8221066.1_g000001 Rmu_sc0017046.1_g000001
rosa_roxburghii	Rroxscaffold_3G00248470 Rroxscaffold_4G00276690
rosa_rugosa	Rorug01G0447800 Rorug07G0120400
rosa_samantha	Rh1AG473100 Rh1BG431100 Rh1CG444200 Rh1DG463600 Rh7AG253200 Rh7BG248200 Rh7CG270100 Rh7DG260700
rosa_wichuraiana	Rw1G039950 Rw7G021580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	703
AatII	GACGTC	1 cut(s)	354
Acc16I	TGCGCA	1 cut(s)	572
Acc36I	ACCTGC	1 cut(s)	703
AccB1I	GGYRCC	2 cut(s)	4, 415
AccII	CGCG	1 cut(s)	849
AclWI	GGATC	1 cut(s)	788
AcoI	YGGCCR	1 cut(s)	582
AcsI	RAATTY	1 cut(s)	760
AcyI	GRCGYC	2 cut(s)	5, 351
AfiI	CCNNNNNNNGG	3 cut(s)	334, 445, 631
AflIII	ACRYGT	1 cut(s)	847
AgeI	ACCGGT	1 cut(s)	46
AgsI	TTSAA	4 cut(s)	162, 368, 643, 874
AjiI	CACGTC	2 cut(s)	667, 883
AjnI	CCWGG	1 cut(s)	940
AluBI	AGCT	6 cut(s)	232, 259, 912, 1042, 1051, 1067
AluI	AGCT	6 cut(s)	232, 259, 912, 1042, 1051, 1067
Alw21I	GWGCWC	2 cut(s)	666, 1044
Alw26I	GTCTC	1 cut(s)	343
AlwI	GGATC	1 cut(s)	788
AlwNI	CAGNNNCTG	1 cut(s)	430
Ama87I	CYCGRG	1 cut(s)	308
AoxI	GGCC	4 cut(s)	205, 238, 582, 853
ApeKI	GCWGC	6 cut(s)	41, 522, 607, 909, 1048, 1067
ApoI	RAATTY	1 cut(s)	760
ArsI	GACNNNNNNTTYG	2 cut(s)	970, 1002
AsiGI	ACCGGT	1 cut(s)	46
AspLEI	GCGC	4 cut(s)	7, 324, 527, 573
AspS9I	GGNCC	5 cut(s)	49, 206, 239, 853, 901
AsuC2I	CCSGG	1 cut(s)	446
AsuHPI	GGTGA	3 cut(s)	349, 505, 727
AsuII	TTCGAA	1 cut(s)	896
AvaI	CYCGRG	1 cut(s)	308
AvaII	GGWCC	2 cut(s)	49, 901
BaeGI	GKGCMC	1 cut(s)	420
BanI	GGYRCC	2 cut(s)	4, 415
BanII	GRGCYC	1 cut(s)	1044
BauI	CACGAG	1 cut(s)	536
BbsI	GAAGAC	1 cut(s)	569
Bbv12I	GWGCWC	2 cut(s)	666, 1044
BbvI	GCAGC	6 cut(s)	53, 534, 594, 921, 1054, 1060
BccI	CCATC	1 cut(s)	323
BceAI	ACGGC	4 cut(s)	154, 253, 490, 643
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciT130I	CCWGG	1 cut(s)	942
BciVI	GTATCC	2 cut(s)	291, 837
BcnI	CCSGG	1 cut(s)	446
BcoDI	GTCTC	1 cut(s)	343
BfaI	CTAG	1 cut(s)	1120
BfmI	CTRYAG	1 cut(s)	1046
BfoI	RGCGCY	3 cut(s)	8, 325, 528
BfuAI	ACCTGC	1 cut(s)	703
BfuI	GTATCC	2 cut(s)	291, 837
BglII	AGATCT	1 cut(s)	408
BisI	GCNGC	7 cut(s)	42, 377, 523, 608, 910, 1049, 1068
BlsI	GCNGC	7 cut(s)	43, 378, 524, 609, 911, 1050, 1069
Bme1390I	CCNGG	2 cut(s)	446, 942
Bme18I	GGWCC	2 cut(s)	49, 901
BmeT110I	CYCGRG	1 cut(s)	308
BmgBI	CACGTC	2 cut(s)	667, 883
BmgT120I	GGNCC	5 cut(s)	49, 206, 239, 853, 901
BmiI	GGNNCC	6 cut(s)	6, 208, 241, 251, 417, 903
BmrFI	CCNGG	2 cut(s)	446, 942
BmsI	GCATC	4 cut(s)	72, 150, 228, 388
BpiI	GAAGAC	1 cut(s)	569
BpmI	CTGGAG	1 cut(s)	836
Bpu14I	TTCGAA	1 cut(s)	896
BpuEI	CTTGAG	2 cut(s)	245, 1037
BpuMI	CCSGG	1 cut(s)	446
Bsa29I	ATCGAT	2 cut(s)	144, 168
BsaBI	GATNNNNATC	2 cut(s)	400, 926
BsaHI	GRCGYC	2 cut(s)	5, 351
BsaI	GGTCTC	1 cut(s)	343
BsaJI	CCNNGG	3 cut(s)	561, 625, 941
BsaWI	WCCGGW	2 cut(s)	46, 490
BsaXI	ACNNNNNCTCC	2 cut(s)	651, 681
Bsc4I	CCNNNNNNNGG	3 cut(s)	334, 445, 631
Bse118I	RCCGGY	1 cut(s)	46
Bse1I	ACTGG	2 cut(s)	251, 670
Bse8I	GATNNNNATC	2 cut(s)	400, 926
BseBI	CCWGG	1 cut(s)	942
BseCI	ATCGAT	2 cut(s)	144, 168
BseDI	CCNNGG	3 cut(s)	561, 625, 941
BseGI	GGATG	3 cut(s)	102, 222, 1015
BseJI	GATNNNNATC	2 cut(s)	400, 926
BseLI	CCNNNNNNNGG	3 cut(s)	334, 445, 631
BseNI	ACTGG	2 cut(s)	251, 670
BseRI	GAGGAG	3 cut(s)	107, 722, 842
BseSI	GKGCMC	1 cut(s)	420
BseXI	GCAGC	6 cut(s)	53, 534, 594, 921, 1054, 1060
BseYI	CCCAGC	1 cut(s)	905
Bsh1236I	CGCG	1 cut(s)	849
BshFI	GGCC	4 cut(s)	207, 240, 584, 855
BshNI	GGYRCC	2 cut(s)	4, 415
BshTI	ACCGGT	1 cut(s)	46
BshVI	ATCGAT	2 cut(s)	144, 168
BsiHKAI	GWGCWC	2 cut(s)	666, 1044
BsiHKCI	CYCGRG	1 cut(s)	308
BsiSI	CCGG	4 cut(s)	47, 446, 491, 695
BslFI	GGGAC	2 cut(s)	137, 582
BslI	CCNNNNNNNGG	3 cut(s)	334, 445, 631
BsmAI	GTCTC	1 cut(s)	343
BsmFI	GGGAC	2 cut(s)	137, 582
BsnI	GGCC	4 cut(s)	207, 240, 584, 855
Bso31I	GGTCTC	1 cut(s)	343
BsoBI	CYCGRG	1 cut(s)	308
Bsp119I	TTCGAA	1 cut(s)	896
Bsp1286I	GDGCHC	3 cut(s)	420, 666, 1044
Bsp143I	GATC	4 cut(s)	195, 408, 793, 921
BspANI	GGCC	4 cut(s)	207, 240, 584, 855
BspDI	ATCGAT	2 cut(s)	144, 168
BspFNI	CGCG	1 cut(s)	849
BspHI	TCATGA	1 cut(s)	790
BspLI	GGNNCC	6 cut(s)	6, 208, 241, 251, 417, 903
BspMAI	CTGCAG	1 cut(s)	1050
BspMI	ACCTGC	1 cut(s)	703
BspPI	GGATC	1 cut(s)	788
BspT104I	TTCGAA	1 cut(s)	896
BspT107I	GGYRCC	2 cut(s)	4, 415
BspTNI	GGTCTC	1 cut(s)	343
BsrFI	RCCGGY	1 cut(s)	46
BsrI	ACTGG	2 cut(s)	251, 670
BssAI	RCCGGY	1 cut(s)	46
BssECI	CCNNGG	3 cut(s)	561, 625, 941
BssMI	GATC	4 cut(s)	195, 408, 793, 921
BssNI	GRCGYC	2 cut(s)	5, 351
BssSI	CACGAG	1 cut(s)	536
BssT1I	CCWWGG	1 cut(s)	561
Bst2BI	CACGAG	1 cut(s)	536
Bst2UI	CCWGG	1 cut(s)	942
Bst4CI	ACNGT	1 cut(s)	615
BstACI	GRCGYC	2 cut(s)	5, 351
BstBI	TTCGAA	1 cut(s)	896
BstC8I	GCNNGC	2 cut(s)	972, 1002
BstDSI	CCRYGG	1 cut(s)	625
BstEII	GGTNACC	1 cut(s)	715
BstF5I	GGATG	3 cut(s)	102, 222, 1015
BstFNI	CGCG	1 cut(s)	849
BstH2I	RGCGCY	3 cut(s)	8, 325, 528
BstHHI	GCGC	4 cut(s)	7, 324, 527, 573
BstKTI	GATC	4 cut(s)	198, 411, 796, 924
BstMAI	GTCTC	1 cut(s)	343
BstMBI	GATC	4 cut(s)	195, 408, 793, 921
BstMWI	GCNNNNNNNGC	2 cut(s)	997, 1048
BstNI	CCWGG	1 cut(s)	942
BstNSI	RCATGY	1 cut(s)	974
BstPI	GGTNACC	1 cut(s)	715
BstSCI	CCNGG	2 cut(s)	444, 940
BstSFI	CTRYAG	1 cut(s)	1046
BstSLI	GKGCMC	1 cut(s)	420
BstUI	CGCG	1 cut(s)	849
BstV1I	GCAGC	6 cut(s)	53, 534, 594, 921, 1054, 1060
BstV2I	GAAGAC	1 cut(s)	569
BstX2I	RGATCY	1 cut(s)	408
BstXI	CCANNNNNNTGG	1 cut(s)	1078
BstYI	RGATCY	1 cut(s)	408
Bsu15I	ATCGAT	2 cut(s)	144, 168
BsuI	GTATCC	2 cut(s)	291, 837
BsuRI	GGCC	4 cut(s)	207, 240, 584, 855
BsuTUI	ATCGAT	2 cut(s)	144, 168
BtgI	CCRYGG	1 cut(s)	625
BtrI	CACGTC	2 cut(s)	667, 883
BtsCI	GGATG	3 cut(s)	102, 222, 1015
BtsI	GCAGTG	2 cut(s)	689, 741
BtsIMutI	CAGTG	4 cut(s)	244, 677, 689, 741
BveI	ACCTGC	1 cut(s)	703
Cac8I	GCNNGC	2 cut(s)	972, 1002
CaiI	CAGNNNCTG	1 cut(s)	430
CciI	TCATGA	1 cut(s)	790
CfoI	GCGC	4 cut(s)	7, 324, 527, 573
Cfr10I	RCCGGY	1 cut(s)	46
Cfr13I	GGNCC	5 cut(s)	49, 206, 239, 853, 901
ClaI	ATCGAT	2 cut(s)	144, 168
CpoI	CGGWCCG	1 cut(s)	49
CseI	GACGC	1 cut(s)	664
CspAI	ACCGGT	1 cut(s)	46
CspI	CGGWCCG	1 cut(s)	49
CviAII	CATG	4 cut(s)	125, 343, 791, 971
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	4 cut(s)	197, 410, 795, 923
DpnII	GATC	4 cut(s)	195, 408, 793, 921
EaeI	YGGCCR	1 cut(s)	582
Ecl136II	GAGCTC	1 cut(s)	1042
Eco130I	CCWWGG	1 cut(s)	561
Eco24I	GRGCYC	1 cut(s)	1044
Eco31I	GGTCTC	1 cut(s)	343
Eco32I	GATATC	1 cut(s)	756
Eco47I	GGWCC	2 cut(s)	49, 901
Eco53kI	GAGCTC	1 cut(s)	1042
Eco88I	CYCGRG	1 cut(s)	308
Eco91I	GGTNACC	1 cut(s)	715
EcoICRI	GAGCTC	1 cut(s)	1042
EcoO109I	RGGNCCY	2 cut(s)	206, 901
EcoO65I	GGTNACC	1 cut(s)	715
EcoRII	CCWGG	1 cut(s)	940
EcoRV	GATATC	1 cut(s)	756
EcoT14I	CCWWGG	1 cut(s)	561
EcoT38I	GRGCYC	1 cut(s)	1044
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	561
FaeI	CATG	4 cut(s)	128, 346, 794, 974
FaiI	YATR	8 cut(s)	72, 126, 344, 780, 792, 948, 972, 1110
FaqI	GGGAC	2 cut(s)	137, 582
FatI	CATG	4 cut(s)	124, 342, 790, 970
FauI	CCCGC	3 cut(s)	312, 469, 623
Fnu4HI	GCNGC	7 cut(s)	42, 377, 523, 608, 910, 1049, 1068
FokI	GGATG	3 cut(s)	89, 209, 1002
FriOI	GRGCYC	1 cut(s)	1044
Fsp4HI	GCNGC	7 cut(s)	42, 377, 523, 608, 910, 1049, 1068
FspBI	CTAG	1 cut(s)	1120
FspI	TGCGCA	1 cut(s)	572
GlaI	GCGC	4 cut(s)	6, 323, 526, 572
GluI	GCNGC	7 cut(s)	42, 377, 523, 608, 910, 1049, 1068
GsaI	CCCAGC	1 cut(s)	909
GsuI	CTGGAG	1 cut(s)	836
HaeII	RGCGCY	3 cut(s)	8, 325, 528
HaeIII	GGCC	4 cut(s)	207, 240, 584, 855
HapII	CCGG	4 cut(s)	47, 446, 491, 695
HgaI	GACGC	1 cut(s)	664
HhaI	GCGC	4 cut(s)	7, 324, 527, 573
Hin1I	GRCGYC	2 cut(s)	5, 351
Hin1II	CATG	4 cut(s)	128, 346, 794, 974
Hin6I	GCGC	4 cut(s)	5, 322, 525, 571
HinP1I	GCGC	4 cut(s)	5, 322, 525, 571
HincII	GTYRAC	1 cut(s)	436
HindII	GTYRAC	1 cut(s)	436
HinfI	GANTC	7 cut(s)	170, 311, 368, 396, 893, 994, 1082
HpaII	CCGG	4 cut(s)	47, 446, 491, 695
HphI	GGTGA	3 cut(s)	349, 505, 727
Hpy166II	GTNNAC	2 cut(s)	436, 622
Hpy188I	TCNGA	4 cut(s)	37, 659, 706, 1087
Hpy188III	TCNNGA	3 cut(s)	299, 791, 800
Hpy8I	GTNNAC	2 cut(s)	436, 622
Hpy99I	CGWCG	3 cut(s)	57, 353, 356
HpyAV	CCTTC	7 cut(s)	85, 109, 220, 443, 722, 832, 892
HpyCH4III	ACNGT	1 cut(s)	615
HpyCH4IV	ACGT	3 cut(s)	351, 666, 882
HpyCH4V	TGCA	5 cut(s)	219, 500, 610, 1004, 1048
HpyF10VI	GCNNNNNNNGC	2 cut(s)	997, 1048
HpySE526I	ACGT	3 cut(s)	351, 666, 882
Hsp92I	GRCGYC	2 cut(s)	5, 351
Hsp92II	CATG	4 cut(s)	128, 346, 794, 974
HspAI	GCGC	4 cut(s)	5, 322, 525, 571
KasI	GGCGCC	1 cut(s)	4
Kzo9I	GATC	4 cut(s)	195, 408, 793, 921
LmnI	GCTCC	3 cut(s)	709, 1039, 1047
Lsp1109I	GCAGC	6 cut(s)	53, 534, 594, 921, 1054, 1060
LweI	GCATC	4 cut(s)	72, 150, 228, 388
MaeI	CTAG	1 cut(s)	1120
MaeII	ACGT	3 cut(s)	351, 666, 882
MaeIII	GTNAC	5 cut(s)	134, 355, 647, 687, 715
MalI	GATC	4 cut(s)	197, 410, 795, 923
MboI	GATC	4 cut(s)	195, 408, 793, 921
MboII	GAAGA	5 cut(s)	418, 467, 569, 1070, 1073
MflI	RGATCY	1 cut(s)	408
MhlI	GDGCHC	3 cut(s)	420, 666, 1044
MluCI	AATT	4 cut(s)	466, 760, 931, 953
MluI	ACGCGT	1 cut(s)	847
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	320, 1091
MmeI	TCCRAC	1 cut(s)	462
MseI	TTAA	2 cut(s)	275, 555
MspA1I	CMGCKG	1 cut(s)	814
MspI	CCGG	4 cut(s)	47, 446, 491, 695
MspR9I	CCNGG	2 cut(s)	446, 942
MvaI	CCWGG	1 cut(s)	942
MvnI	CGCG	1 cut(s)	849
MwoI	GCNNNNNNNGC	2 cut(s)	997, 1048
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	446
NdeII	GATC	4 cut(s)	195, 408, 793, 921
NlaIII	CATG	4 cut(s)	128, 346, 794, 974
NlaIV	GGNNCC	6 cut(s)	6, 208, 241, 251, 417, 903
NmuCI	GTSAC	3 cut(s)	355, 687, 715
NsbI	TGCGCA	1 cut(s)	572
NspI	RCATGY	1 cut(s)	974
NspV	TTCGAA	1 cut(s)	896
PaeI	GCATGC	1 cut(s)	974
PagI	TCATGA	1 cut(s)	790
PaqCI	CACCTGC	1 cut(s)	703
PcsI	WCGNNNNNNNCGW	2 cut(s)	663, 672
PfeI	GAWTC	5 cut(s)	170, 368, 396, 893, 994
PflFI	GACNNNGTC	1 cut(s)	353
PinAI	ACCGGT	1 cut(s)	46
PkrI	GCNGC	7 cut(s)	43, 378, 524, 609, 911, 1050, 1069
PleI	GAGTC	2 cut(s)	319, 1090
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	319, 1090
PpuMI	RGGWCCY	1 cut(s)	901
Psp124BI	GAGCTC	1 cut(s)	1044
Psp5II	RGGWCCY	1 cut(s)	901
Psp6I	CCWGG	1 cut(s)	940
PspEI	GGTNACC	1 cut(s)	715
PspFI	CCCAGC	1 cut(s)	905
PspGI	CCWGG	1 cut(s)	940
PspN4I	GGNNCC	6 cut(s)	6, 208, 241, 251, 417, 903
PspPI	GGNCC	5 cut(s)	49, 206, 239, 853, 901
PspPPI	RGGWCCY	1 cut(s)	901
PstI	CTGCAG	1 cut(s)	1050
PstNI	CAGNNNCTG	1 cut(s)	430
PsuI	RGATCY	1 cut(s)	408
PsyI	GACNNNGTC	1 cut(s)	353
Rsr2I	CGGWCCG	1 cut(s)	49
RsrII	CGGWCCG	1 cut(s)	49
SacI	GAGCTC	1 cut(s)	1044
SaqAI	TTAA	2 cut(s)	275, 555
SatI	GCNGC	7 cut(s)	42, 377, 523, 608, 910, 1049, 1068
Sau3AI	GATC	4 cut(s)	195, 408, 793, 921
Sau96I	GGNCC	5 cut(s)	49, 206, 239, 853, 901
SchI	GAGTC	2 cut(s)	320, 1091
ScrFI	CCNGG	2 cut(s)	446, 942
SduI	GDGCHC	3 cut(s)	420, 666, 1044
SfaNI	GCATC	4 cut(s)	72, 150, 228, 388
SfcI	CTRYAG	1 cut(s)	1046
SfoI	GGCGCC	1 cut(s)	6
SfuI	TTCGAA	1 cut(s)	896
SinI	GGWCC	2 cut(s)	49, 901
SmlI	CTYRAG	2 cut(s)	260, 1052
SmoI	CTYRAG	2 cut(s)	260, 1052
SphI	GCATGC	1 cut(s)	974
Sse9I	AATT	4 cut(s)	466, 760, 931, 953
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	1 cut(s)	1120
SstI	GAGCTC	1 cut(s)	1044
StyD4I	CCNGG	2 cut(s)	444, 940
StyI	CCWWGG	1 cut(s)	561
TaaI	ACNGT	1 cut(s)	615
TaiI	ACGT	3 cut(s)	354, 669, 885
TasI	AATT	4 cut(s)	466, 760, 931, 953
TauI	GCSGC	1 cut(s)	379
TfiI	GAWTC	5 cut(s)	170, 368, 396, 893, 994
Tru1I	TTAA	2 cut(s)	275, 555
Tru9I	TTAA	2 cut(s)	275, 555
TscAI	CASTG	4 cut(s)	251, 677, 696, 741
TseFI	GTSAC	3 cut(s)	355, 687, 715
TseI	GCWGC	6 cut(s)	41, 522, 607, 909, 1048, 1067
Tsp45I	GTSAC	3 cut(s)	355, 687, 715
TspDTI	ATGAA	1 cut(s)	91
TspGWI	ACGGA	1 cut(s)	41
TspRI	CASTG	4 cut(s)	251, 677, 696, 741
Tth111I	GACNNNGTC	1 cut(s)	353
VpaK11BI	GGWCC	2 cut(s)	49, 901
XapI	RAATTY	1 cut(s)	760
XceI	RCATGY	1 cut(s)	974
XcmI	CCANNNNNNNNNTGG	1 cut(s)	427
XspI	CTAG	1 cut(s)	1120
ZraI	GACGTC	1 cut(s)	352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.