Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp02

Orthogroup ID

OG0004084

Physical Location & Seq

Genomic Coordinates Reverse (-)

30172721 .. 30174873

Gene Structure 2153 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.2G327800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGAAAATTGAATCATCATCAGACGCAGCAGCAGCCCAACGCTTCCGCATCGGGTACGCCTTTGCACCCAAGAAGGAGCAGAGCTTCATCCAGCCCTCTCTCCTCGACCATGCCTCCCAAAACGGCATTGATTTCGTCCCAATTTCAATAACCGACTCCTCGTCGGAGGAGGAGGCGGCCGAGGCCTTGATCCAACAAGGCCCATTCCACTGCATCATCCACAAGCTCTACGGCCACCGCTGGAACCACCAGTTGAAGCAATACTCCTCCAAGTATCCACAAACTCTCATACTTGACCCGCCCGACTCCATTGAGCGCCTCCACAACAGGGTCTCCATGCTCCAAGTCGTCAGCGCCATCAAATTGAATTCCCAATTTAACATCTCTGTCTCTGTGCCTAAACAGGTGGTGCTTCAAACCCCCCAGTCGCCTGATTCTATTCATAATAATACTAATGATGGTGCGGTGGAGTTTCCGGTGATTGCCAAGCCCTTGCTGGCCAATGGCAGCGCCAAGTCGCATGAGATGTATTTGGTTTTTGACCCCAAGGGCTTGCAGACTCTTCTTCCCACTAATACTACTACTAGTGCTAGTACGCAACCCATTTTGCTTCAACAGTTTGTGAACCACGGCGAGGTCGTCTTCAAGGTTTACGTCATTGGCGAATACGTCCAGTGCGTCAAGCGGAGCTCCTTGCCCGATATTTCTGAGCAGCAGCTGACCGCATCGGAAGGGCAAGTGTTGCGGTTTTCGCAGATATCGAATTCCCCTCAGCCTCAGGAGGAGGATGAACATGGTCTGGGTCTGGCTCATGATCCTCATGAAATGCCTCCTTCTGACTTTGTGGAGGAGCTGGCCAGGGGGATACGCCTCGGCCTCAAACTCAACTTCTTTAACTTTGATGTGATTCGGGATTCTGGGAACCCTCACTCCTATTTTGTCATTGACATCAATTACTTTCCTGGCTATGCCAAGTTGCCCTCCTATGAGCAGGTCTTGACCAACTTTCTGTTGGCTCTTCTTACCACTCCAACCCAAAAGGACCACCAGCTGGAACCAGCTACCACTGAGGAGGAGGAGGAGGAGGAGGAGAAGGAGGAGTCTGATCACGACCACTTAGGAGACGGAGAAGGAGGAGGAGATGCTTCCCTCCTACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006020 GO:0006066 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010264 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0019637 GO:0019751 GO:0032958 GO:0033517 GO:0043647 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046173 GO:0046835 GO:0051766 GO:0052725 GO:0052726 GO:0052746 GO:0071704 GO:0090407 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Ins134_P3_kin

Protein Analysis

387

Amino Acids

42.97

Weight (kDa)

4.89

Isoelectric Point (pI)

57.66

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004084)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16760
fragaria_vesca	FvH4_5g22490 FvH4_7g34131
malus_domestica	MD01G1237400.v1.1 MD03G1144600.v1.1 MD06G1037200.v1.1 MD07G1309900.v1.1 MD16G1271300.v1.1
prunus_persica	Prupe.2G327800_v2.0.a1 Prupe.5G031800_v2.0.a1
pyrus_communis	pycom06g03060 pycom07g28190 pycom16g24080
rosa_chinensis	RchiOBHm_Chr1g0384451 RchiOBHm_Chr7g0210271
rosa_laevigata	RLG00000003053 RLG00000026039
rosa_multiflora	Rmu_co8221066.1_g000001 Rmu_sc0017046.1_g000001
rosa_roxburghii	Rroxscaffold_3G00248470 Rroxscaffold_4G00276690
rosa_rugosa	Rorug01G0447800 Rorug07G0120400
rosa_samantha	Rh1AG473100 Rh1BG431100 Rh1CG444200 Rh1DG463600 Rh7AG253200 Rh7BG248200 Rh7CG270100 Rh7DG260700
rosa_wichuraiana	Rw1G039950 Rw7G021580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	982
AccB7I	CCANNNNNTGG	1 cut(s)	1051
AciI	CCGC	8 cut(s)	46, 176, 238, 299, 464, 685, 723, 745
AclWI	GGATC	2 cut(s)	184, 809
AcoI	YGGCCR	4 cut(s)	177, 232, 498, 855
AcsI	RAATTY	2 cut(s)	367, 763
AfaI	GTAC	2 cut(s)	56, 595
AfiI	CCNNNNNNNGG	3 cut(s)	328, 634, 1051
AgsI	TTSAA	7 cut(s)	11, 147, 256, 367, 416, 614, 646
AhdI	GACNNNNNGTC	1 cut(s)	160
AhlI	ACTAGT	1 cut(s)	584
AjnI	CCWGG	2 cut(s)	857, 961
AloI	GAACNNNNNNTCC	2 cut(s)	914, 946
AluBI	AGCT	7 cut(s)	84, 226, 690, 718, 853, 1051, 1061
AluI	AGCT	7 cut(s)	84, 226, 690, 718, 853, 1051, 1061
Alw21I	GWGCWC	1 cut(s)	692
Alw26I	GTCTC	3 cut(s)	337, 394, 1116
AlwI	GGATC	2 cut(s)	184, 809
AlwNI	CAGNNNCTG	1 cut(s)	718
AoxI	GGCC	7 cut(s)	177, 183, 199, 232, 498, 855, 874
ApeKI	GCWGC	6 cut(s)	26, 29, 32, 507, 712, 715
ApoI	RAATTY	2 cut(s)	367, 763
AspLEI	GCGC	3 cut(s)	318, 356, 512
AspS9I	GGNCC	2 cut(s)	200, 1042
AsuHPI	GGTGA	1 cut(s)	490
AvaII	GGWCC	1 cut(s)	1042
AxyI	CCTNAGG	1 cut(s)	777
BalI	TGGCCA	2 cut(s)	500, 857
BanII	GRGCYC	1 cut(s)	692
BbsI	GAAGAC	1 cut(s)	634
Bbv12I	GWGCWC	1 cut(s)	692
BbvCI	CCTCAGC	1 cut(s)	771
BbvI	GCAGC	6 cut(s)	38, 41, 44, 519, 724, 727
BccI	CCATC	2 cut(s)	365, 452
BceAI	ACGGC	3 cut(s)	139, 247, 646
BcgI	CGANNNNNNTGC	2 cut(s)	115, 149
BciT130I	CCWGG	2 cut(s)	859, 963
BciVI	GTATCC	2 cut(s)	285, 858
BclI	TGATCA	1 cut(s)	1105
BcoDI	GTCTC	3 cut(s)	337, 394, 1116
BcuI	ACTAGT	1 cut(s)	584
BfaI	CTAG	2 cut(s)	585, 591
BfoI	RGCGCY	3 cut(s)	319, 357, 513
BfuAI	ACCTGC	1 cut(s)	982
BfuI	GTATCC	2 cut(s)	285, 858
BisI	GCNGC	7 cut(s)	27, 30, 33, 177, 508, 713, 716
BlsI	GCNGC	7 cut(s)	28, 31, 34, 178, 509, 714, 717
Bme1390I	CCNGG	2 cut(s)	859, 963
Bme18I	GGWCC	1 cut(s)	1042
BmeRI	GACNNNNNGTC	1 cut(s)	160
BmgT120I	GGNCC	2 cut(s)	200, 1042
BmiI	GGNNCC	3 cut(s)	245, 923, 1056
BmrFI	CCNGG	2 cut(s)	859, 963
BmrI	ACTGGG	1 cut(s)	418
BmsI	GCATC	4 cut(s)	57, 222, 734, 1132
BmuI	ACTGGG	1 cut(s)	418
BpiI	GAAGAC	1 cut(s)	634
Bpu10I	CCTNAGC	1 cut(s)	771
BsaI	GGTCTC	1 cut(s)	337
BsaJI	CCNNGG	5 cut(s)	180, 546, 628, 858, 871
BsaWI	WCCGGW	1 cut(s)	475
BsaXI	ACNNNNNCTCC	6 cut(s)	98, 128, 317, 347, 914, 944
Bsc4I	CCNNNNNNNGG	3 cut(s)	328, 634, 1051
Bse1I	ACTGG	3 cut(s)	250, 424, 673
Bse21I	CCTNAGG	1 cut(s)	777
BseBI	CCWGG	2 cut(s)	859, 963
BseDI	CCNNGG	5 cut(s)	180, 546, 628, 858, 871
BseGI	GGATG	3 cut(s)	87, 216, 793
BseLI	CCNNNNNNNGG	3 cut(s)	328, 634, 1051
BseMII	CTCAG	4 cut(s)	699, 785, 791, 1059
BseNI	ACTGG	3 cut(s)	250, 424, 673
BseX3I	CGGCCG	1 cut(s)	177
BseXI	GCAGC	6 cut(s)	38, 41, 44, 519, 724, 727
Bsh1285I	CGRYCG	1 cut(s)	180
BshFI	GGCC	7 cut(s)	179, 185, 201, 234, 500, 857, 876
BsiEI	CGRYCG	1 cut(s)	180
BsiHKAI	GWGCWC	1 cut(s)	692
BsiSI	CCGG	1 cut(s)	476
BslFI	GGGAC	1 cut(s)	122
BslI	CCNNNNNNNGG	3 cut(s)	328, 634, 1051
BsmAI	GTCTC	3 cut(s)	337, 394, 1116
BsmBI	CGTCTC	1 cut(s)	1116
BsmFI	GGGAC	1 cut(s)	122
BsnI	GGCC	7 cut(s)	179, 185, 201, 234, 500, 857, 876
Bso31I	GGTCTC	1 cut(s)	337
Bsp1286I	GDGCHC	1 cut(s)	692
Bsp143I	GATC	3 cut(s)	189, 814, 1105
BspACI	CCGC	8 cut(s)	46, 176, 238, 299, 464, 685, 723, 745
BspANI	GGCC	7 cut(s)	179, 185, 201, 234, 500, 857, 876
BspCNI	CTCAG	4 cut(s)	700, 784, 790, 1060
BspHI	TCATGA	2 cut(s)	811, 820
BspLI	GGNNCC	3 cut(s)	245, 923, 1056
BspMI	ACCTGC	1 cut(s)	982
BspPI	GGATC	2 cut(s)	184, 809
BspQI	GCTCTTC	1 cut(s)	1023
BspTNI	GGTCTC	1 cut(s)	337
BsrI	ACTGG	3 cut(s)	250, 424, 673
BssECI	CCNNGG	5 cut(s)	180, 546, 628, 858, 871
BssMI	GATC	3 cut(s)	189, 814, 1105
BssT1I	CCWWGG	1 cut(s)	546
Bst2UI	CCWGG	2 cut(s)	859, 963
Bst4CI	ACNGT	1 cut(s)	618
Bst6I	CTCTTC	2 cut(s)	567, 1023
BstAPI	GCANNNNNTGC	1 cut(s)	742
BstC8I	GCNNGC	3 cut(s)	498, 554, 855
BstDEI	CTNAG	5 cut(s)	708, 771, 777, 1068, 1117
BstDSI	CCRYGG	1 cut(s)	628
BstF5I	GGATG	3 cut(s)	87, 216, 793
BstH2I	RGCGCY	3 cut(s)	319, 357, 513
BstHHI	GCGC	3 cut(s)	318, 356, 512
BstKTI	GATC	3 cut(s)	192, 817, 1108
BstMAI	GTCTC	3 cut(s)	337, 394, 1116
BstMBI	GATC	3 cut(s)	189, 814, 1105
BstMCI	CGRYCG	1 cut(s)	180
BstMWI	GCNNNNNNNGC	4 cut(s)	32, 182, 742, 751
BstNI	CCWGG	2 cut(s)	859, 963
BstSCI	CCNGG	2 cut(s)	857, 961
BstV1I	GCAGC	6 cut(s)	38, 41, 44, 519, 724, 727
BstV2I	GAAGAC	1 cut(s)	634
BstZI	CGGCCG	1 cut(s)	177
Bsu36I	CCTNAGG	1 cut(s)	777
BsuI	GTATCC	2 cut(s)	285, 858
BsuRI	GGCC	7 cut(s)	179, 185, 201, 234, 500, 857, 876
BtgI	CCRYGG	1 cut(s)	628
BtsCI	GGATG	3 cut(s)	87, 216, 793
BtsI	GCAGTG	1 cut(s)	208
BtsIMutI	CAGTG	3 cut(s)	208, 680, 1065
BveI	ACCTGC	1 cut(s)	982
Cac8I	GCNNGC	3 cut(s)	498, 554, 855
CaiI	CAGNNNCTG	1 cut(s)	718
CciI	TCATGA	2 cut(s)	811, 820
CfoI	GCGC	3 cut(s)	318, 356, 512
Cfr13I	GGNCC	2 cut(s)	200, 1042
CseI	GACGC	2 cut(s)	32, 667
Csp6I	GTAC	2 cut(s)	55, 594
CviAII	CATG	6 cut(s)	110, 337, 521, 794, 812, 821
CviQI	GTAC	2 cut(s)	55, 594
DdeI	CTNAG	5 cut(s)	708, 771, 777, 1068, 1117
DpnI	GATC	3 cut(s)	191, 816, 1107
DpnII	GATC	3 cut(s)	189, 814, 1105
DriI	GACNNNNNGTC	1 cut(s)	160
EaeI	YGGCCR	4 cut(s)	177, 232, 498, 855
EagI	CGGCCG	1 cut(s)	177
Eam1104I	CTCTTC	2 cut(s)	567, 1023
Eam1105I	GACNNNNNGTC	1 cut(s)	160
EarI	CTCTTC	2 cut(s)	567, 1023
Ecl136II	GAGCTC	1 cut(s)	690
EclXI	CGGCCG	1 cut(s)	177
Eco130I	CCWWGG	1 cut(s)	546
Eco147I	AGGCCT	1 cut(s)	185
Eco24I	GRGCYC	1 cut(s)	692
Eco31I	GGTCTC	1 cut(s)	337
Eco32I	GATATC	1 cut(s)	759
Eco47I	GGWCC	1 cut(s)	1042
Eco52I	CGGCCG	1 cut(s)	177
Eco53kI	GAGCTC	1 cut(s)	690
Eco81I	CCTNAGG	1 cut(s)	777
EcoICRI	GAGCTC	1 cut(s)	690
EcoRI	GAATTC	2 cut(s)	367, 763
EcoRII	CCWGG	2 cut(s)	857, 961
EcoRV	GATATC	1 cut(s)	759
EcoT14I	CCWWGG	1 cut(s)	546
EcoT38I	GRGCYC	1 cut(s)	692
ErhI	CCWWGG	1 cut(s)	546
Esp3I	CGTCTC	1 cut(s)	1116
FaeI	CATG	6 cut(s)	113, 340, 524, 797, 815, 824
FaqI	GGGAC	1 cut(s)	122
FatI	CATG	6 cut(s)	109, 336, 520, 793, 811, 820
FauI	CCCGC	1 cut(s)	306
FbaI	TGATCA	1 cut(s)	1105
Fnu4HI	GCNGC	7 cut(s)	27, 30, 33, 177, 508, 713, 716
FokI	GGATG	3 cut(s)	74, 203, 800
FriOI	GRGCYC	1 cut(s)	692
Fsp4HI	GCNGC	7 cut(s)	27, 30, 33, 177, 508, 713, 716
FspBI	CTAG	2 cut(s)	585, 591
GlaI	GCGC	3 cut(s)	317, 355, 511
GluI	GCNGC	7 cut(s)	27, 30, 33, 177, 508, 713, 716
HaeII	RGCGCY	3 cut(s)	319, 357, 513
HaeIII	GGCC	7 cut(s)	179, 185, 201, 234, 500, 857, 876
HapII	CCGG	1 cut(s)	476
HgaI	GACGC	2 cut(s)	32, 667
HhaI	GCGC	3 cut(s)	318, 356, 512
Hin1II	CATG	6 cut(s)	113, 340, 524, 797, 815, 824
Hin6I	GCGC	3 cut(s)	316, 354, 510
HinP1I	GCGC	3 cut(s)	316, 354, 510
HinfI	GANTC	8 cut(s)	11, 155, 305, 434, 559, 907, 914, 1100
HpaII	CCGG	1 cut(s)	476
HphI	GGTGA	1 cut(s)	490
Hpy166II	GTNNAC	2 cut(s)	625, 652
Hpy188I	TCNGA	6 cut(s)	22, 166, 709, 730, 838, 1105
Hpy188III	TCNNGA	6 cut(s)	779, 812, 821, 911, 997, 1109
Hpy8I	GTNNAC	2 cut(s)	625, 652
Hpy99I	CGWCG	1 cut(s)	166
HpyAV	CCTTC	5 cut(s)	67, 725, 843, 1087, 1124
HpyCH4III	ACNGT	1 cut(s)	618
HpyCH4IV	ACGT	2 cut(s)	654, 669
HpyCH4V	TGCA	3 cut(s)	65, 213, 556
HpyF10VI	GCNNNNNNNGC	4 cut(s)	32, 182, 742, 751
HpyF3I	CTNAG	5 cut(s)	708, 771, 777, 1068, 1117
HpySE526I	ACGT	2 cut(s)	654, 669
Hsp92II	CATG	6 cut(s)	113, 340, 524, 797, 815, 824
HspAI	GCGC	3 cut(s)	316, 354, 510
Ksp22I	TGATCA	1 cut(s)	1105
Kzo9I	GATC	3 cut(s)	189, 814, 1105
LguI	GCTCTTC	1 cut(s)	1023
LmnI	GCTCC	5 cut(s)	76, 345, 687, 695, 850
Lsp1109I	GCAGC	6 cut(s)	38, 41, 44, 519, 724, 727
LweI	GCATC	4 cut(s)	57, 222, 734, 1132
MaeI	CTAG	2 cut(s)	585, 591
MaeII	ACGT	2 cut(s)	654, 669
MalI	GATC	3 cut(s)	191, 816, 1107
MboI	GATC	3 cut(s)	189, 814, 1105
MboII	GAAGA	4 cut(s)	554, 557, 634, 1010
MhlI	GDGCHC	1 cut(s)	692
MlsI	TGGCCA	2 cut(s)	500, 857
MluCI	AATT	7 cut(s)	6, 141, 362, 367, 374, 763, 952
MluNI	TGGCCA	2 cut(s)	500, 857
MlyI	GAGTC	4 cut(s)	149, 299, 553, 1109
MmeI	TCCRAC	3 cut(s)	144, 217, 1055
Mox20I	TGGCCA	2 cut(s)	500, 857
MscI	TGGCCA	2 cut(s)	500, 857
MseI	TTAA	2 cut(s)	378, 894
Msp20I	TGGCCA	2 cut(s)	500, 857
MspA1I	CMGCKG	3 cut(s)	240, 718, 1051
MspI	CCGG	1 cut(s)	476
MspR9I	CCNGG	2 cut(s)	859, 963
MvaI	CCWGG	2 cut(s)	859, 963
MwoI	GCNNNNNNNGC	4 cut(s)	32, 182, 742, 751
NdeII	GATC	3 cut(s)	189, 814, 1105
NlaIII	CATG	6 cut(s)	113, 340, 524, 797, 815, 824
NlaIV	GGNNCC	3 cut(s)	245, 923, 1056
NmeAIII	GCCGAG	2 cut(s)	205, 852
PagI	TCATGA	2 cut(s)	811, 820
PceI	AGGCCT	1 cut(s)	185
PciSI	GCTCTTC	1 cut(s)	1023
PcsI	WCGNNNNNNNCGW	4 cut(s)	636, 660, 675, 758
PfeI	GAWTC	4 cut(s)	11, 434, 907, 914
PflMI	CCANNNNNTGG	1 cut(s)	1051
PkrI	GCNGC	7 cut(s)	28, 31, 34, 178, 509, 714, 717
PleI	GAGTC	4 cut(s)	149, 299, 553, 1108
PpsI	GAGTC	4 cut(s)	149, 299, 553, 1108
Psp124BI	GAGCTC	1 cut(s)	692
Psp6I	CCWGG	2 cut(s)	857, 961
PspGI	CCWGG	2 cut(s)	857, 961
PspN4I	GGNNCC	3 cut(s)	245, 923, 1056
PspPI	GGNCC	2 cut(s)	200, 1042
PstNI	CAGNNNCTG	1 cut(s)	718
PvuII	CAGCTG	2 cut(s)	718, 1051
RsaI	GTAC	2 cut(s)	56, 595
RsaNI	GTAC	2 cut(s)	55, 594
SacI	GAGCTC	1 cut(s)	692
SapI	GCTCTTC	1 cut(s)	1023
SaqAI	TTAA	2 cut(s)	378, 894
SatI	GCNGC	7 cut(s)	27, 30, 33, 177, 508, 713, 716
Sau3AI	GATC	3 cut(s)	189, 814, 1105
Sau96I	GGNCC	2 cut(s)	200, 1042
SchI	GAGTC	4 cut(s)	149, 299, 553, 1109
ScrFI	CCNGG	2 cut(s)	859, 963
SduI	GDGCHC	1 cut(s)	692
SfaNI	GCATC	4 cut(s)	57, 222, 734, 1132
SinI	GGWCC	1 cut(s)	1042
SpeI	ACTAGT	1 cut(s)	584
Sse9I	AATT	7 cut(s)	6, 141, 362, 367, 374, 763, 952
SseBI	AGGCCT	1 cut(s)	185
SsiI	CCGC	8 cut(s)	46, 176, 238, 299, 464, 685, 723, 745
SspMI	CTAG	2 cut(s)	585, 591
SstI	GAGCTC	1 cut(s)	692
StuI	AGGCCT	1 cut(s)	185
StyD4I	CCNGG	2 cut(s)	857, 961
StyI	CCWWGG	1 cut(s)	546
TaaI	ACNGT	1 cut(s)	618
TaiI	ACGT	2 cut(s)	657, 672
TaqI	TCGA	2 cut(s)	105, 761
TasI	AATT	7 cut(s)	6, 141, 362, 367, 374, 763, 952
TauI	GCSGC	1 cut(s)	179
TfiI	GAWTC	4 cut(s)	11, 434, 907, 914
Tru1I	TTAA	2 cut(s)	378, 894
Tru9I	TTAA	2 cut(s)	378, 894
TscAI	CASTG	3 cut(s)	215, 680, 1072
TseI	GCWGC	6 cut(s)	26, 29, 32, 507, 712, 715
TspDTI	ATGAA	5 cut(s)	17, 76, 431, 804, 837
TspGWI	ACGGA	1 cut(s)	1140
TspRI	CASTG	3 cut(s)	215, 680, 1072
Van91I	CCANNNNNTGG	1 cut(s)	1051
VpaK11BI	GGWCC	1 cut(s)	1042
XapI	RAATTY	2 cut(s)	367, 763
XcmI	CCANNNNNNNNNTGG	2 cut(s)	493, 1009
XspI	CTAG	2 cut(s)	585, 591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.