Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0004084

Physical Location & Seq

Genomic Coordinates Reverse (-)

27606490 .. 27607581

Gene Structure 1092 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g28190.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGATCCAAGCACAAAGTCACTAGTATCAGAATCAGAAGCAGCACCGGTCCGTCGCTACCGCATCGGGTACGCCTTCGCACCCAAAAAAGAGCAGACCTTCATCCAGCCCTCTCTCCTCCACCATGCCTCCCGATACGGCATCGATTTCGTCCCCATTTCAATCCCCGATTCCACCACCACCGCCAAGACCCTGATCGATCAAGTCCCCTTCGATTGCATCATCCACAAGATCTACGGCCCCCACTGGAACCAACACCTCAAGGAATACTCGTCCCGGTATCCACAAACGCTCATTCTTGACCCGCCCGAGTCCATCGAGCGCCTCCACAATCGGGTCTCCATGCTCGACGTCTTCGCCGCCTTTGAATTGAAGCCGCGTCAGTCAGGCGACAAGTTCCAATCGAAGATCTCGGTGCCCAAACAGGTGCCGGTTGATCATTACCCGGAAGGCGAAGAAGAAACTAAGTTGTTGGCCGGGTTGGAGTTTCCGGTGATTGCAAAGCCATTACTGGCGAATGGCAGCACCAAGTCGCACGAGATGTGTTTGATTTTTAGCCCCAAGGGGTTGCGCAGTCTTCTAGCGGATACTAATAGTCCCATTTTGCTGCAACAGTTTGCGAACCACGGCGGGGTGGTGTTCAAGGGTTACGCGATTGGGGAGCACGTCCAGTGCGTCAAGCGGAGGTCGCTGCCGGACATTTCGAAGGAGCGGGTGACCGCATTAGAAGAAGCGGTGCTTCCGTTTTCGCAGATATCAAATTCGGCGAATGACGAGCATAATCTGGCTCATGATCCTCACGAAATGCCTCCTCTGGAGTTTGTTGAGGAGGCGGCGAAGAGGATACGCGTGGGCCTCAAACTCAACTTCTTCAACTTTGACATGATAAGAGATTCGAAGGACCCCAACAGCTATTTTGTGATCGACATCAACTACTTTCCTGGGTATGCCAAATTGCCTAACTATGAGCATGCCTTTACAGACTTTTTGCTGAATCTGCTTTCAACTAGTAGCCATCCCCAAAAGCAAAAGGAGCTGCTGCAGCTCAAGGAAGAAGAAGGTGCTACCAGTTTCGAGTCCGATGTCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006020 GO:0006066 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010264 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0019637 GO:0019751 GO:0032958 GO:0033517 GO:0043647 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046173 GO:0046835 GO:0051766 GO:0052725 GO:0052726 GO:0052746 GO:0071704 GO:0090407 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Ins134_P3_kin

Protein Analysis

364

Amino Acids

41.1

Weight (kDa)

6.11

Isoelectric Point (pI)

50.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ins134_P3_kin_N	PF17927	21 - 106	4.8e-22	Inositol 1,3,4-trisphosphate 5/6-kinase pre-ATP-grasp domain
Ins134_P3_kin	PF05770	134 - 331	1.4e-64	Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004084)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16760
fragaria_vesca	FvH4_5g22490 FvH4_7g34131
malus_domestica	MD01G1237400.v1.1 MD03G1144600.v1.1 MD06G1037200.v1.1 MD07G1309900.v1.1 MD16G1271300.v1.1
prunus_persica	Prupe.2G327800_v2.0.a1 Prupe.5G031800_v2.0.a1
pyrus_communis	pycom06g03060 pycom07g28190 pycom16g24080
rosa_chinensis	RchiOBHm_Chr1g0384451 RchiOBHm_Chr7g0210271
rosa_laevigata	RLG00000003053 RLG00000026039
rosa_multiflora	Rmu_co8221066.1_g000001 Rmu_sc0017046.1_g000001
rosa_roxburghii	Rroxscaffold_3G00248470 Rroxscaffold_4G00276690
rosa_rugosa	Rorug01G0447800 Rorug07G0120400
rosa_samantha	Rh1AG473100 Rh1BG431100 Rh1CG444200 Rh1DG463600 Rh7AG253200 Rh7BG248200 Rh7CG270100 Rh7DG260700
rosa_wichuraiana	Rw1G039950 Rw7G021580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	354
Acc16I	TGCGCA	1 cut(s)	572
AccB1I	GGYRCC	2 cut(s)	415, 427
AccBSI	CCGCTC	1 cut(s)	712
AccII	CGCG	3 cut(s)	379, 653, 849
AclWI	GGATC	2 cut(s)	12, 788
AcoI	YGGCCR	1 cut(s)	474
AcsI	RAATTY	1 cut(s)	760
AcyI	GRCGYC	1 cut(s)	351
AfaI	GTAC	1 cut(s)	71
AfiI	CCNNNNNNNGG	2 cut(s)	334, 631
AflIII	ACRYGT	1 cut(s)	847
AgeI	ACCGGT	1 cut(s)	46
AgsI	TTSAA	6 cut(s)	162, 368, 373, 643, 874, 1005
AhlI	ACTAGT	2 cut(s)	22, 1007
AjiI	CACGTC	1 cut(s)	667
AjnI	CCWGG	1 cut(s)	940
AluBI	AGCT	3 cut(s)	912, 1036, 1045
AluI	AGCT	3 cut(s)	912, 1036, 1045
Alw21I	GWGCWC	1 cut(s)	666
Alw26I	GTCTC	1 cut(s)	343
AlwI	GGATC	2 cut(s)	12, 788
Ama87I	CYCGRG	1 cut(s)	308
AoxI	GGCC	3 cut(s)	238, 474, 853
ApeKI	GCWGC	7 cut(s)	41, 522, 607, 691, 1036, 1039, 1042
ApoI	RAATTY	1 cut(s)	760
AsiGI	ACCGGT	1 cut(s)	46
AspLEI	GCGC	2 cut(s)	324, 573
AspS9I	GGNCC	4 cut(s)	49, 239, 853, 901
AsuC2I	CCSGG	3 cut(s)	277, 446, 478
AsuHPI	GGTGA	2 cut(s)	505, 727
AsuII	TTCGAA	2 cut(s)	704, 896
AvaI	CYCGRG	1 cut(s)	308
AvaII	GGWCC	2 cut(s)	49, 901
BaeGI	GKGCMC	1 cut(s)	420
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	2 cut(s)	415, 427
BauI	CACGAG	1 cut(s)	536
BbsI	GAAGAC	2 cut(s)	346, 569
Bbv12I	GWGCWC	1 cut(s)	666
BbvI	GCAGC	7 cut(s)	53, 534, 594, 678, 1023, 1026, 1054
BccI	CCATC	2 cut(s)	323, 1023
BceAI	ACGGC	3 cut(s)	154, 253, 643
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciT130I	CCWGG	1 cut(s)	942
BciVI	GTATCC	3 cut(s)	291, 580, 837
BclI	TGATCA	1 cut(s)	436
BcnI	CCSGG	3 cut(s)	277, 446, 478
BcoDI	GTCTC	1 cut(s)	343
BcuI	ACTAGT	2 cut(s)	22, 1007
BfaI	CTAG	3 cut(s)	23, 581, 1008
BfmI	CTRYAG	1 cut(s)	1040
BfoI	RGCGCY	1 cut(s)	325
BfuI	GTATCC	3 cut(s)	291, 580, 837
BglII	AGATCT	2 cut(s)	231, 408
Bme1390I	CCNGG	4 cut(s)	277, 446, 478, 942
Bme18I	GGWCC	2 cut(s)	49, 901
BmeT110I	CYCGRG	1 cut(s)	308
BmgBI	CACGTC	1 cut(s)	667
BmgT120I	GGNCC	4 cut(s)	49, 239, 853, 901
BmiI	GGNNCC	6 cut(s)	6, 241, 251, 417, 429, 903
BmrFI	CCNGG	4 cut(s)	277, 446, 478, 942
BmsI	GCATC	3 cut(s)	72, 150, 228
BpiI	GAAGAC	2 cut(s)	346, 569
BpmI	CTGGAG	1 cut(s)	836
Bpu14I	TTCGAA	2 cut(s)	704, 896
BpuEI	CTTGAG	2 cut(s)	245, 1031
BpuMI	CCSGG	3 cut(s)	277, 446, 478
Bsa29I	ATCGAT	2 cut(s)	144, 198
BsaBI	GATNNNNATC	1 cut(s)	926
BsaHI	GRCGYC	1 cut(s)	351
BsaI	GGTCTC	1 cut(s)	343
BsaJI	CCNNGG	3 cut(s)	561, 625, 941
BsaWI	WCCGGW	2 cut(s)	46, 490
BsaXI	ACNNNNNCTCC	2 cut(s)	113, 143
Bsc4I	CCNNNNNNNGG	2 cut(s)	334, 631
Bse118I	RCCGGY	2 cut(s)	46, 430
Bse1I	ACTGG	4 cut(s)	251, 516, 670, 1068
Bse8I	GATNNNNATC	1 cut(s)	926
BseBI	CCWGG	1 cut(s)	942
BseCI	ATCGAT	2 cut(s)	144, 198
BseDI	CCNNGG	3 cut(s)	561, 625, 941
BseGI	GGATG	3 cut(s)	102, 222, 1015
BseJI	GATNNNNATC	1 cut(s)	926
BseLI	CCNNNNNNNGG	2 cut(s)	334, 631
BseNI	ACTGG	4 cut(s)	251, 516, 670, 1068
BseRI	GAGGAG	3 cut(s)	107, 801, 842
BseSI	GKGCMC	1 cut(s)	420
BseXI	GCAGC	7 cut(s)	53, 534, 594, 678, 1023, 1026, 1054
Bsh1236I	CGCG	3 cut(s)	379, 653, 849
BshFI	GGCC	3 cut(s)	240, 476, 855
BshNI	GGYRCC	2 cut(s)	415, 427
BshTI	ACCGGT	1 cut(s)	46
BshVI	ATCGAT	2 cut(s)	144, 198
BsiHKAI	GWGCWC	1 cut(s)	666
BsiHKCI	CYCGRG	1 cut(s)	308
BsiSI	CCGG	7 cut(s)	47, 277, 431, 446, 477, 491, 695
BslFI	GGGAC	4 cut(s)	137, 191, 259, 582
BslI	CCNNNNNNNGG	2 cut(s)	334, 631
BsmAI	GTCTC	1 cut(s)	343
BsmFI	GGGAC	4 cut(s)	137, 191, 259, 582
BsnI	GGCC	3 cut(s)	240, 476, 855
Bso31I	GGTCTC	1 cut(s)	343
BsoBI	CYCGRG	1 cut(s)	308
Bsp119I	TTCGAA	2 cut(s)	704, 896
Bsp1286I	GDGCHC	2 cut(s)	420, 666
Bsp143I	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
BspANI	GGCC	3 cut(s)	240, 476, 855
BspDI	ATCGAT	2 cut(s)	144, 198
BspFNI	CGCG	3 cut(s)	379, 653, 849
BspHI	TCATGA	1 cut(s)	790
BspLI	GGNNCC	6 cut(s)	6, 241, 251, 417, 429, 903
BspMAI	CTGCAG	1 cut(s)	1044
BspPI	GGATC	2 cut(s)	12, 788
BspT104I	TTCGAA	2 cut(s)	704, 896
BspT107I	GGYRCC	2 cut(s)	415, 427
BspTNI	GGTCTC	1 cut(s)	343
BsrBI	CCGCTC	1 cut(s)	712
BsrFI	RCCGGY	2 cut(s)	46, 430
BsrI	ACTGG	4 cut(s)	251, 516, 670, 1068
BssAI	RCCGGY	2 cut(s)	46, 430
BssECI	CCNNGG	3 cut(s)	561, 625, 941
BssMI	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
BssNI	GRCGYC	1 cut(s)	351
BssSI	CACGAG	1 cut(s)	536
BssT1I	CCWWGG	1 cut(s)	561
Bst2BI	CACGAG	1 cut(s)	536
Bst2UI	CCWGG	1 cut(s)	942
Bst4CI	ACNGT	1 cut(s)	615
Bst6I	CTCTTC	1 cut(s)	833
BstACI	GRCGYC	1 cut(s)	351
BstBI	TTCGAA	2 cut(s)	704, 896
BstC8I	GCNNGC	1 cut(s)	972
BstDEI	CTNAG	1 cut(s)	465
BstDSI	CCRYGG	1 cut(s)	625
BstEII	GGTNACC	1 cut(s)	715
BstF5I	GGATG	3 cut(s)	102, 222, 1015
BstFNI	CGCG	3 cut(s)	379, 653, 849
BstH2I	RGCGCY	1 cut(s)	325
BstHHI	GCGC	2 cut(s)	324, 573
BstKTI	GATC	8 cut(s)	7, 198, 202, 234, 411, 439, 796, 924
BstMAI	GTCTC	1 cut(s)	343
BstMBI	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
BstMWI	GCNNNNNNNGC	4 cut(s)	688, 997, 1033, 1042
BstNI	CCWGG	1 cut(s)	942
BstNSI	RCATGY	1 cut(s)	974
BstPI	GGTNACC	1 cut(s)	715
BstSCI	CCNGG	4 cut(s)	275, 444, 476, 940
BstSFI	CTRYAG	1 cut(s)	1040
BstSLI	GKGCMC	1 cut(s)	420
BstUI	CGCG	3 cut(s)	379, 653, 849
BstV1I	GCAGC	7 cut(s)	53, 534, 594, 678, 1023, 1026, 1054
BstV2I	GAAGAC	2 cut(s)	346, 569
BstX2I	RGATCY	3 cut(s)	4, 231, 408
BstYI	RGATCY	3 cut(s)	4, 231, 408
Bsu15I	ATCGAT	2 cut(s)	144, 198
BsuI	GTATCC	3 cut(s)	291, 580, 837
BsuRI	GGCC	3 cut(s)	240, 476, 855
BsuTUI	ATCGAT	2 cut(s)	144, 198
BtgI	CCRYGG	1 cut(s)	625
BtrI	CACGTC	1 cut(s)	667
BtsCI	GGATG	3 cut(s)	102, 222, 1015
BtsIMutI	CAGTG	2 cut(s)	244, 677
Cac8I	GCNNGC	1 cut(s)	972
CciI	TCATGA	1 cut(s)	790
CfoI	GCGC	2 cut(s)	324, 573
Cfr10I	RCCGGY	2 cut(s)	46, 430
Cfr13I	GGNCC	4 cut(s)	49, 239, 853, 901
ClaI	ATCGAT	2 cut(s)	144, 198
CpoI	CGGWCCG	1 cut(s)	49
CseI	GACGC	2 cut(s)	368, 664
Csp6I	GTAC	1 cut(s)	70
CspAI	ACCGGT	1 cut(s)	46
CspI	CGGWCCG	1 cut(s)	49
CviAII	CATG	5 cut(s)	125, 343, 791, 883, 971
CviQI	GTAC	1 cut(s)	70
DdeI	CTNAG	1 cut(s)	465
DpnI	GATC	8 cut(s)	6, 197, 201, 233, 410, 438, 795, 923
DpnII	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
EaeI	YGGCCR	1 cut(s)	474
Eam1104I	CTCTTC	1 cut(s)	833
EarI	CTCTTC	1 cut(s)	833
Eco130I	CCWWGG	1 cut(s)	561
Eco31I	GGTCTC	1 cut(s)	343
Eco32I	GATATC	1 cut(s)	756
Eco47I	GGWCC	2 cut(s)	49, 901
Eco88I	CYCGRG	1 cut(s)	308
Eco91I	GGTNACC	1 cut(s)	715
EcoO109I	RGGNCCY	1 cut(s)	901
EcoO65I	GGTNACC	1 cut(s)	715
EcoRII	CCWGG	1 cut(s)	940
EcoRV	GATATC	1 cut(s)	756
EcoT14I	CCWWGG	1 cut(s)	561
ErhI	CCWWGG	1 cut(s)	561
FaeI	CATG	5 cut(s)	128, 346, 794, 886, 974
FaiI	YATR	8 cut(s)	126, 344, 780, 792, 884, 948, 966, 972
FalI	AAGNNNNNCTT	2 cut(s)	723, 755
FaqI	GGGAC	4 cut(s)	137, 191, 259, 582
FatI	CATG	5 cut(s)	124, 342, 790, 882, 970
FauI	CCCGC	3 cut(s)	312, 623, 705
FbaI	TGATCA	1 cut(s)	436
FokI	GGATG	3 cut(s)	89, 209, 1002
FspBI	CTAG	3 cut(s)	23, 581, 1008
FspI	TGCGCA	1 cut(s)	572
GlaI	GCGC	2 cut(s)	323, 572
GsuI	CTGGAG	1 cut(s)	836
HaeII	RGCGCY	1 cut(s)	325
HaeIII	GGCC	3 cut(s)	240, 476, 855
HapII	CCGG	7 cut(s)	47, 277, 431, 446, 477, 491, 695
HgaI	GACGC	2 cut(s)	368, 664
HhaI	GCGC	2 cut(s)	324, 573
Hin1I	GRCGYC	1 cut(s)	351
Hin1II	CATG	5 cut(s)	128, 346, 794, 886, 974
Hin6I	GCGC	2 cut(s)	322, 571
HinP1I	GCGC	2 cut(s)	322, 571
HinfI	GANTC	6 cut(s)	32, 170, 311, 893, 994, 1076
HpaII	CCGG	7 cut(s)	47, 277, 431, 446, 477, 491, 695
HphI	GGTGA	2 cut(s)	505, 727
Hpy188I	TCNGA	3 cut(s)	31, 37, 1081
Hpy188III	TCNNGA	5 cut(s)	132, 299, 791, 800, 815
Hpy99I	CGWCG	2 cut(s)	57, 353
HpyAV	CCTTC	7 cut(s)	85, 109, 220, 443, 700, 892, 1052
HpyCH4III	ACNGT	1 cut(s)	615
HpyCH4IV	ACGT	2 cut(s)	351, 666
HpyCH4V	TGCA	4 cut(s)	219, 500, 610, 1042
HpyF10VI	GCNNNNNNNGC	4 cut(s)	688, 997, 1033, 1042
HpyF3I	CTNAG	1 cut(s)	465
HpySE526I	ACGT	2 cut(s)	351, 666
Hsp92I	GRCGYC	1 cut(s)	351
Hsp92II	CATG	5 cut(s)	128, 346, 794, 886, 974
HspAI	GCGC	2 cut(s)	322, 571
Ksp22I	TGATCA	1 cut(s)	436
Kzo9I	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
LmnI	GCTCC	3 cut(s)	661, 709, 1033
Lsp1109I	GCAGC	7 cut(s)	53, 534, 594, 678, 1023, 1026, 1054
LweI	GCATC	3 cut(s)	72, 150, 228
MaeI	CTAG	3 cut(s)	23, 581, 1008
MaeII	ACGT	2 cut(s)	351, 666
MaeIII	GTNAC	3 cut(s)	18, 647, 715
MalI	GATC	8 cut(s)	6, 197, 201, 233, 410, 438, 795, 923
MbiI	CCGCTC	1 cut(s)	712
MboI	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
MflI	RGATCY	3 cut(s)	4, 231, 408
MhlI	GDGCHC	2 cut(s)	420, 666
MluCI	AATT	3 cut(s)	368, 760, 953
MluI	ACGCGT	1 cut(s)	847
MlyI	GAGTC	2 cut(s)	320, 1085
MmeI	TCCRAC	1 cut(s)	462
MspI	CCGG	7 cut(s)	47, 277, 431, 446, 477, 491, 695
MspR9I	CCNGG	4 cut(s)	277, 446, 478, 942
MvaI	CCWGG	1 cut(s)	942
MvnI	CGCG	3 cut(s)	379, 653, 849
MwoI	GCNNNNNNNGC	4 cut(s)	688, 997, 1033, 1042
NciI	CCSGG	3 cut(s)	277, 446, 478
NdeII	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
NlaIII	CATG	5 cut(s)	128, 346, 794, 886, 974
NlaIV	GGNNCC	6 cut(s)	6, 241, 251, 417, 429, 903
NmuCI	GTSAC	2 cut(s)	18, 715
NsbI	TGCGCA	1 cut(s)	572
NspI	RCATGY	1 cut(s)	974
NspV	TTCGAA	2 cut(s)	704, 896
PaeI	GCATGC	1 cut(s)	974
PagI	TCATGA	1 cut(s)	790
PcsI	WCGNNNNNNNCGW	1 cut(s)	672
PfeI	GAWTC	4 cut(s)	32, 170, 893, 994
PinAI	ACCGGT	1 cut(s)	46
PleI	GAGTC	2 cut(s)	319, 1084
PpsI	GAGTC	2 cut(s)	319, 1084
PpuMI	RGGWCCY	1 cut(s)	901
Psp5II	RGGWCCY	1 cut(s)	901
Psp6I	CCWGG	1 cut(s)	940
PspEI	GGTNACC	1 cut(s)	715
PspGI	CCWGG	1 cut(s)	940
PspN4I	GGNNCC	6 cut(s)	6, 241, 251, 417, 429, 903
PspPI	GGNCC	4 cut(s)	49, 239, 853, 901
PspPPI	RGGWCCY	1 cut(s)	901
PstI	CTGCAG	1 cut(s)	1044
PsuI	RGATCY	3 cut(s)	4, 231, 408
RsaI	GTAC	1 cut(s)	71
RsaNI	GTAC	1 cut(s)	70
Rsr2I	CGGWCCG	1 cut(s)	49
RsrII	CGGWCCG	1 cut(s)	49
Sau3AI	GATC	8 cut(s)	4, 195, 199, 231, 408, 436, 793, 921
Sau96I	GGNCC	4 cut(s)	49, 239, 853, 901
SchI	GAGTC	2 cut(s)	320, 1085
ScrFI	CCNGG	4 cut(s)	277, 446, 478, 942
SduI	GDGCHC	2 cut(s)	420, 666
SfaNI	GCATC	3 cut(s)	72, 150, 228
SfcI	CTRYAG	1 cut(s)	1040
SfuI	TTCGAA	2 cut(s)	704, 896
SinI	GGWCC	2 cut(s)	49, 901
SmlI	CTYRAG	2 cut(s)	260, 1046
SmoI	CTYRAG	2 cut(s)	260, 1046
SpeI	ACTAGT	2 cut(s)	22, 1007
SphI	GCATGC	1 cut(s)	974
Sse9I	AATT	3 cut(s)	368, 760, 953
SspMI	CTAG	3 cut(s)	23, 581, 1008
StyD4I	CCNGG	4 cut(s)	275, 444, 476, 940
StyI	CCWWGG	1 cut(s)	561
TaaI	ACNGT	1 cut(s)	615
TaiI	ACGT	2 cut(s)	354, 669
TasI	AATT	3 cut(s)	368, 760, 953
TauI	GCSGC	3 cut(s)	362, 379, 836
TfiI	GAWTC	4 cut(s)	32, 170, 893, 994
TscAI	CASTG	2 cut(s)	251, 677
TseFI	GTSAC	2 cut(s)	18, 715
TseI	GCWGC	7 cut(s)	41, 522, 607, 691, 1036, 1039, 1042
Tsp45I	GTSAC	2 cut(s)	18, 715
TspDTI	ATGAA	1 cut(s)	91
TspGWI	ACGGA	2 cut(s)	41, 732
TspRI	CASTG	2 cut(s)	251, 677
VpaK11BI	GGWCC	2 cut(s)	49, 901
XapI	RAATTY	1 cut(s)	760
XceI	RCATGY	1 cut(s)	974
XspI	CTAG	3 cut(s)	23, 581, 1008
ZraI	GACGTC	1 cut(s)	352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.