Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011053

Physical Location & Seq

Genomic Coordinates Reverse (-)

32441365 .. 32445817

Gene Structure 4453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1237500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGTCTGTGGGACCCATGAAAACCTTTGCACCCCTTTCCTTCCCTACCACTTTTGAGGGCGGATGCGGAGTCGATTTGAATTTCCCCACCACCACCACCGTCATGGATTCCTCTGGTCCTGCCCTCGCCCTCGCCACCACTTCAATTGCAGAGCCTCATGACGCCATGGAGTTTGAATCTCATGAAGCCGCATACACCTTCTACAAAGCATATGCCAAGTCCGTGGGTTTCGGCACTGCTAAGTTGAGTAGTCGTCGCTCTAGAGCATCCAAGGAGTTCATTGATGCCAAATTTTCATGCATCCGGTACGGAAACAAGCAGCAGTCCGATGATGCTATCAATCCTCGACCTTCTCCCAAAATTGGCTGCAAGGCGAGCATGCATGTCAAGCGCAGGCCAAATGGTAATTGGTATGTTTATAGTTTCGTAAAGGACCACAACCATGAGCTTTTACCAGATCAAGCACATTTCTTTCGCAGCCACAGAAACACTGACCCCCTTAACAATGATGTTAGAATCCGGAGACGCAAGAGTTTAGCTGCAGTGTCCAGCCTCTTCAGTGCCTATCAAAATGTTGATTGTCTAGAGAGTTACTTGAGAAATCAGCATGACAAGGGACGGATTTTGGTTTTAGAAGCAGGGGATGCTCAACTGCTGCTTGAATATTTTATGCGCCTACAGGAAGACAACCCAAAATTCTTCTATGCAGTTGATCTGAATGAAGAGCATAGGTTGAGAAATGTGTTCTGGGTTGATGCCAAAGCAATGGAAGATTACACTAACTTCAACGATGTTGTTTTCTTTGACACCACATACTTCACAAACAAGTATAAAATACCGCTGGTTCTTTTTATAGGAGTGAACCATCACATTCAGCCCACATTACTTGGCTGTGCTTTGATTGCAGATGATACGGTTTATACTTTTATGTGGCTAATGCAAACATGGTTTATAGCAATGGGTGAACAAGCTCCACGAGTAATACTCACCGACCAGAACGACGCCATCAAAGCGGCTATTCCAGCAGTATTTCCAGGCACACGCCATTGCTTTTCTTTATGGAACATAATGGAGAAGATGCACAGGCAGCTCGAGTTTCTAAGCATGTGGCATGATAGTTTTTTGGAAAAATTTACTAAGTGTATATTCAAGTCCTGGTCAGAACAGCAATTTGAAAAGAGATGGTGGAAACTTATTGATAGATTTAATCTTAGGGAGGTTGGATGGATGCAGTCATTGTATGAAGATCGTACTCATTGGGCCCCTACGTTCATGAGAGATATATCCTTCGCTGGGTTGTCTCCAACTTCACGGTCAGAAAGTTTGAACTCTTCGTTTGACAAATATATTCAAGGGGATACTTCACTGCAAGAGTTTATGGAAAGATACACAGCAATTCTTGAAGATAGGTATGAAGAGGAAGCGAAAGCAAATTTTGATGCTTGGCATGGAACACCTGAGTTAAAGTCTCCATCGCCGTTTGAGAAACAAATGTCACTAGTGTACACACACGAGATTTTCAAAAACTTTCAAGTTGAGGTTTTGGGAGCGGCTGCTTGTCATCTTAAGAAAGAAAATGAAGATGAGACAGCAACAACGTTCAGTGTCAAAGACTTTGAAGACAATCAGAATTATACCGTAGAGTGGAATGAATCAAAATCAGATATATATTGTTCTTGCCACTCATTTGAGTATAAAGGCTATCTCTGTAGACATGCTATTGTTGTTCTCCAAATGTCTGGTGTTTTCATCATCCCTCCCAAATATATATTGCAGCGATGGACAAATGCTGCTATGAGCAGGCATACCATTGGTGAAAGAGTGGATGAGGTTCAGTCTAAAGCCCGTCGCTACAATGATTTATGTCGACGGGCCATAATATTGGGTGAAGAGGGGTCACTCTCTCAAGAAAGTTATGATGTAGCATTATCGGCCATAAAAGAAGCGTTGAAGCAATGTGCAAGTTTAAACAACTGTCTTGAGAACAATGCAAAACCTCATGATTCAGCAATACATGGTGTTTGCGGTGTTGATGAAGAGAATCAATGCAGCACTACATCTGGTGCCAACAAGACTCCTAGGAGAGCCGGGTCCGGGAAGGAGGTGGCTAGGAATGGGAATAGCACAAGCAAGAAAGGAAAGGTACCTCAGCTGGAAGCTATGAGCTTTGGAATGCAAGACGGCCATTTCCAAATGCAACTTTCTGACTCAAGGCCCATGCAGTTGCATAATGCGGTGCCAACAATGTTTCAGAGTGTTGCATCAACACAGCTCCATAATGTGGCATCAACACATGTGCATGAAAATCTCCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009628 GO:0009639 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010017 GO:0010018 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042752 GO:0042753 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051606 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0080090 GO:0104004 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAR1 MULE SWIM

Protein Analysis

773

Amino Acids

88.38

Weight (kDa)

6.35

Isoelectric Point (pI)

48.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAR1	PF03101	66 - 152	1.8e-28	FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like	PF21056	235 - 348	8.9e-06	Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE	PF10551	265 - 359	1.2e-25	MULE transposase domain
SWIM	PF04434	545 - 579	5.3e-09	SWIM zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76320 AT1G76320 AT1G76320 AT1G76320
fragaria_vesca	FvH4_7g34132
malus_domestica	MD01G1237500.v1.1 MD07G1310000.v1.1
prunus_persica	Prupe.2G327900_v2.0.a1
pyrus_communis	pycom01g24520
rosa_chinensis	RchiOBHm_Chr1g0384461
rosa_multiflora	Rmu_co8407315.1_g000001
rosa_roxburghii	Rroxscaffold_4G00276680
rosa_rugosa	Rorug01G0447900 Rorug01G0448000 Rorug05G0131300
rosa_samantha	Rh1AG473200 Rh1BG431200 Rh1CG444400
rosa_wichuraiana	Rw1G039940 Rw4G020470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2143
AccB1I	GGYRCC	3 cut(s)	2063, 2143, 2236
AccB7I	CCANNNNNTGG	1 cut(s)	2281
AccBSI	CCGCTC	1 cut(s)	1550
AccI	GTMKAC	2 cut(s)	1711, 1867
AccIII	TCCGGA	1 cut(s)	519
AciI	CCGC	8 cut(s)	60, 66, 189, 839, 1013, 1550, 2025, 2234
AclI	AACGTT	1 cut(s)	1598
AcoI	YGGCCR	2 cut(s)	1932, 2182
AcsI	RAATTY	5 cut(s)	79, 290, 695, 1130, 1432
AcuI	CTGAAG	1 cut(s)	541
AcyI	GRCGYC	2 cut(s)	162, 1002
AfaI	GTAC	4 cut(s)	308, 1252, 1505, 2145
AfiI	CCNNNNNNNGG	5 cut(s)	362, 1293, 1644, 2094, 2281
AflII	CTTAAG	1 cut(s)	1565
AflIII	ACRYGT	1 cut(s)	2293
AhlI	ACTAGT	1 cut(s)	1498
AjnI	CCWGG	2 cut(s)	1033, 1154
AluBI	AGCT	8 cut(s)	448, 539, 971, 1090, 2152, 2159, 2166, 2272
AluI	AGCT	8 cut(s)	448, 539, 971, 1090, 2152, 2159, 2166, 2272
Alw26I	GTCTC	4 cut(s)	517, 1305, 1473, 1580
Ama87I	CYCGRG	1 cut(s)	1091
Aor13HI	TCCGGA	1 cut(s)	519
AoxI	GGCC	6 cut(s)	395, 1260, 1872, 1932, 2182, 2213
ApaI	GGGCCC	1 cut(s)	1264
ApoI	RAATTY	5 cut(s)	79, 290, 695, 1130, 1432
Asp718I	GGTACC	1 cut(s)	2143
AspA2I	CCTAGG	1 cut(s)	2078
AspLEI	GCGC	2 cut(s)	393, 675
AspS9I	GGNCC	8 cut(s)	11, 116, 433, 1260, 1261, 1872, 2091, 2214
AsuC2I	CCSGG	2 cut(s)	2089, 2095
AsuHPI	GGTGA	4 cut(s)	974, 979, 1826, 1898
AvaI	CYCGRG	1 cut(s)	1091
AvaII	GGWCC	4 cut(s)	11, 116, 433, 2091
AvrII	CCTAGG	1 cut(s)	2078
BaeGI	GKGCMC	1 cut(s)	1264
BanI	GGYRCC	3 cut(s)	2063, 2143, 2236
BanII	GRGCYC	1 cut(s)	1264
BauI	CACGAG	2 cut(s)	975, 1511
BbsI	GAAGAC	2 cut(s)	690, 1626
BbvCI	CCTCAGC	1 cut(s)	2148
BccI	CCATC	6 cut(s)	873, 1013, 1176, 1218, 1480, 1773
BceAI	ACGGC	2 cut(s)	1462, 2197
BciT130I	CCWGG	2 cut(s)	1035, 1156
BciVI	GTATCC	1 cut(s)	1351
BcnI	CCSGG	2 cut(s)	2089, 2095
BcoDI	GTCTC	4 cut(s)	517, 1305, 1473, 1580
BcuI	ACTAGT	1 cut(s)	1498
BfaI	CTAG	5 cut(s)	261, 584, 1499, 2079, 2109
BfmI	CTRYAG	3 cut(s)	540, 677, 1708
BfrI	CTTAAG	1 cut(s)	1565
BfuI	GTATCC	1 cut(s)	1351
BlnI	CCTAGG	1 cut(s)	2078
Bme1390I	CCNGG	4 cut(s)	1035, 1156, 2089, 2095
Bme18I	GGWCC	4 cut(s)	11, 116, 433, 2091
BmeT110I	CYCGRG	1 cut(s)	1091
BmgT120I	GGNCC	8 cut(s)	11, 116, 433, 1260, 1261, 1872, 2091, 2214
BmiI	GGNNCC	8 cut(s)	12, 13, 1262, 1263, 2065, 2092, 2145, 2238
BmrFI	CCNGG	4 cut(s)	1035, 1156, 2089, 2095
BpiI	GAAGAC	2 cut(s)	690, 1626
BplI	GAGNNNNNCTC	2 cut(s)	969, 1001
Bpu10I	CCTNAGC	1 cut(s)	2148
BpuEI	CTTGAG	4 cut(s)	616, 1890, 2000, 2194
BpuMI	CCSGG	2 cut(s)	2089, 2095
BsaHI	GRCGYC	2 cut(s)	162, 1002
BsaJI	CCNNGG	4 cut(s)	165, 222, 270, 2078
BsaWI	WCCGGW	2 cut(s)	303, 519
Bsc4I	CCNNNNNNNGG	5 cut(s)	362, 1293, 1644, 2094, 2281
Bse3DI	GCAATG	4 cut(s)	771, 963, 1045, 1961
BseAI	TCCGGA	1 cut(s)	519
BseBI	CCWGG	2 cut(s)	1035, 1156
BseDI	CCNNGG	4 cut(s)	165, 222, 270, 2078
BseGI	GGATG	8 cut(s)	68, 266, 300, 649, 1229, 1233, 1752, 1831
BseLI	CCNNNNNNNGG	5 cut(s)	362, 1293, 1644, 2094, 2281
BseMI	GCAATG	4 cut(s)	771, 963, 1045, 1961
BseMII	CTCAG	2 cut(s)	1449, 2162
BseSI	GKGCMC	1 cut(s)	1264
BseYI	CCCAGC	1 cut(s)	1292
BshFI	GGCC	6 cut(s)	397, 1262, 1874, 1934, 2184, 2215
BshNI	GGYRCC	3 cut(s)	2063, 2143, 2236
BsiHKCI	CYCGRG	1 cut(s)	1091
BsiSI	CCGG	4 cut(s)	304, 520, 2088, 2094
BslFI	GGGAC	2 cut(s)	24, 630
BslI	CCNNNNNNNGG	5 cut(s)	362, 1293, 1644, 2094, 2281
BsmAI	GTCTC	4 cut(s)	517, 1305, 1473, 1580
BsmBI	CGTCTC	1 cut(s)	517
BsmFI	GGGAC	2 cut(s)	24, 630
BsmI	GAATGC	1 cut(s)	2178
BsnI	GGCC	6 cut(s)	397, 1262, 1874, 1934, 2184, 2215
BsoBI	CYCGRG	1 cut(s)	1091
Bsp120I	GGGCCC	1 cut(s)	1260
Bsp1286I	GDGCHC	1 cut(s)	1264
Bsp13I	TCCGGA	1 cut(s)	519
Bsp1407I	TGTACA	1 cut(s)	1503
Bsp143I	GATC	3 cut(s)	457, 712, 1246
Bsp19I	CCATGG	1 cut(s)	165
BspACI	CCGC	8 cut(s)	60, 66, 189, 839, 1013, 1550, 2025, 2234
BspANI	GGCC	6 cut(s)	397, 1262, 1874, 1934, 2184, 2215
BspCNI	CTCAG	2 cut(s)	1450, 2161
BspEI	TCCGGA	1 cut(s)	519
BspHI	TCATGA	4 cut(s)	157, 181, 1272, 1999
BspLI	GGNNCC	8 cut(s)	12, 13, 1262, 1263, 2065, 2092, 2145, 2238
BspMAI	CTGCAG	1 cut(s)	544
BspQI	GCTCTTC	1 cut(s)	717
BspT107I	GGYRCC	3 cut(s)	2063, 2143, 2236
BspTI	CTTAAG	1 cut(s)	1565
BsrBI	CCGCTC	1 cut(s)	1550
BsrDI	GCAATG	4 cut(s)	771, 963, 1045, 1961
BsrGI	TGTACA	1 cut(s)	1503
BssECI	CCNNGG	4 cut(s)	165, 222, 270, 2078
BssMI	GATC	3 cut(s)	457, 712, 1246
BssNI	GRCGYC	2 cut(s)	162, 1002
BssSI	CACGAG	2 cut(s)	975, 1511
BssT1I	CCWWGG	3 cut(s)	165, 270, 2078
Bst2BI	CACGAG	2 cut(s)	975, 1511
Bst2UI	CCWGG	2 cut(s)	1035, 1156
Bst4CI	ACNGT	5 cut(s)	100, 916, 1314, 1639, 1976
Bst6I	CTCTTC	6 cut(s)	560, 717, 1336, 1410, 1884, 2031
BstACI	GRCGYC	2 cut(s)	162, 1002
BstAFI	CTTAAG	1 cut(s)	1565
BstAPI	GCANNNNNTGC	1 cut(s)	644
BstAUI	TGTACA	1 cut(s)	1503
BstC8I	GCNNGC	4 cut(s)	376, 380, 395, 1802
BstDEI	CTNAG	7 cut(s)	240, 1100, 1137, 1211, 1458, 2148, 2313
BstDSI	CCRYGG	2 cut(s)	165, 222
BstF5I	GGATG	8 cut(s)	68, 266, 300, 649, 1229, 1233, 1752, 1831
BstHHI	GCGC	2 cut(s)	393, 675
BstKTI	GATC	3 cut(s)	460, 715, 1249
BstMAI	GTCTC	4 cut(s)	517, 1305, 1473, 1580
BstMBI	GATC	3 cut(s)	457, 712, 1246
BstMWI	GCNNNNNNNGC	8 cut(s)	375, 388, 644, 902, 1010, 1022, 1087, 1931
BstNI	CCWGG	2 cut(s)	1035, 1156
BstNSI	RCATGY	5 cut(s)	382, 386, 1108, 1718, 2297
BstSCI	CCNGG	4 cut(s)	1033, 1154, 2087, 2093
BstSFI	CTRYAG	3 cut(s)	540, 677, 1708
BstSLI	GKGCMC	1 cut(s)	1264
BstV2I	GAAGAC	2 cut(s)	690, 1626
BstXI	CCANNNNNNTGG	5 cut(s)	103, 223, 766, 1739, 1882
BsuI	GTATCC	1 cut(s)	1351
BsuRI	GGCC	6 cut(s)	397, 1262, 1874, 1934, 2184, 2215
BtgI	CCRYGG	2 cut(s)	165, 222
BtgZI	GCGATG	2 cut(s)	1458, 1792
BtsCI	GGATG	8 cut(s)	68, 266, 300, 649, 1229, 1233, 1752, 1831
BtsI	GCAGTG	3 cut(s)	234, 549, 1364
BtsIMutI	CAGTG	6 cut(s)	234, 489, 549, 565, 1364, 1609
Cac8I	GCNNGC	4 cut(s)	376, 380, 395, 1802
CciI	TCATGA	4 cut(s)	157, 181, 1272, 1999
CfoI	GCGC	2 cut(s)	393, 675
Cfr13I	GGNCC	8 cut(s)	11, 116, 433, 1260, 1261, 1872, 2091, 2214
CseI	GACGC	3 cut(s)	170, 534, 1010
Csp6I	GTAC	4 cut(s)	307, 1251, 1504, 2144
CspCI	CAANNNNNGTGG	4 cut(s)	127, 162, 868, 903
CviQI	GTAC	4 cut(s)	307, 1251, 1504, 2144
DdeI	CTNAG	7 cut(s)	240, 1100, 1137, 1211, 1458, 2148, 2313
DpnI	GATC	3 cut(s)	459, 714, 1248
DpnII	GATC	3 cut(s)	457, 712, 1246
DraI	TTTAAA	1 cut(s)	1968
EaeI	YGGCCR	2 cut(s)	1932, 2182
Eam1104I	CTCTTC	6 cut(s)	560, 717, 1336, 1410, 1884, 2031
EarI	CTCTTC	6 cut(s)	560, 717, 1336, 1410, 1884, 2031
EciI	GGCGGA	1 cut(s)	75
Eco130I	CCWWGG	3 cut(s)	165, 270, 2078
Eco24I	GRGCYC	1 cut(s)	1264
Eco47I	GGWCC	4 cut(s)	11, 116, 433, 2091
Eco57I	CTGAAG	1 cut(s)	541
Eco88I	CYCGRG	1 cut(s)	1091
EcoO109I	RGGNCCY	2 cut(s)	11, 1261
EcoRII	CCWGG	2 cut(s)	1033, 1154
EcoT14I	CCWWGG	3 cut(s)	165, 270, 2078
EcoT22I	ATGCAT	2 cut(s)	302, 384
EcoT38I	GRGCYC	1 cut(s)	1264
ErhI	CCWWGG	3 cut(s)	165, 270, 2078
Esp3I	CGTCTC	1 cut(s)	517
FaqI	GGGAC	2 cut(s)	24, 630
FauNDI	CATATG	1 cut(s)	211
FblI	GTMKAC	2 cut(s)	1711, 1867
FokI	GGATG	8 cut(s)	75, 253, 287, 656, 1236, 1240, 1739, 1838
FriOI	GRGCYC	1 cut(s)	1264
FspBI	CTAG	5 cut(s)	261, 584, 1499, 2079, 2109
GlaI	GCGC	2 cut(s)	392, 674
GsaI	CCCAGC	1 cut(s)	1296
HaeIII	GGCC	6 cut(s)	397, 1262, 1874, 1934, 2184, 2215
HapII	CCGG	4 cut(s)	304, 520, 2088, 2094
HgaI	GACGC	3 cut(s)	170, 534, 1010
HhaI	GCGC	2 cut(s)	393, 675
Hin1I	GRCGYC	2 cut(s)	162, 1002
Hin6I	GCGC	2 cut(s)	391, 673
HinP1I	GCGC	2 cut(s)	391, 673
HincII	GTYRAC	1 cut(s)	1868
HindII	GTYRAC	1 cut(s)	1868
HinfI	GANTC	9 cut(s)	69, 107, 176, 516, 1652, 2003, 2041, 2074, 2207
HpaII	CCGG	4 cut(s)	304, 520, 2088, 2094
HphI	GGTGA	4 cut(s)	974, 979, 1826, 1898
Hpy166II	GTNNAC	6 cut(s)	862, 965, 1504, 1506, 1712, 1868
Hpy188I	TCNGA	8 cut(s)	328, 717, 1162, 1318, 1629, 1663, 2206, 2253
Hpy8I	GTNNAC	6 cut(s)	862, 965, 1504, 1506, 1712, 1868
Hpy99I	CGWCG	4 cut(s)	258, 1004, 1851, 1872
HpyAV	CCTTC	5 cut(s)	49, 208, 360, 1297, 2092
HpyCH4III	ACNGT	5 cut(s)	100, 916, 1314, 1639, 1976
HpyCH4IV	ACGT	2 cut(s)	1268, 1598
HpyF10VI	GCNNNNNNNGC	8 cut(s)	375, 388, 644, 902, 1010, 1022, 1087, 1931
HpyF3I	CTNAG	7 cut(s)	240, 1100, 1137, 1211, 1458, 2148, 2313
HpySE526I	ACGT	2 cut(s)	1268, 1598
Hsp92I	GRCGYC	2 cut(s)	162, 1002
HspAI	GCGC	2 cut(s)	391, 673
KflI	GGGWCCC	1 cut(s)	11
Kpn2I	TCCGGA	1 cut(s)	519
KpnI	GGTACC	1 cut(s)	2147
Kzo9I	GATC	3 cut(s)	457, 712, 1246
LguI	GCTCTTC	1 cut(s)	717
LmnI	GCTCC	3 cut(s)	976, 1547, 2277
MaeI	CTAG	5 cut(s)	261, 584, 1499, 2079, 2109
MaeII	ACGT	2 cut(s)	1268, 1598
MaeIII	GTNAC	3 cut(s)	590, 1494, 1896
MalI	GATC	3 cut(s)	459, 714, 1248
MbiI	CCGCTC	1 cut(s)	1550
MboI	GATC	3 cut(s)	457, 712, 1246
MfeI	CAATTG	1 cut(s)	144
MhlI	GDGCHC	1 cut(s)	1264
MlyI	GAGTC	3 cut(s)	78, 2068, 2201
MmeI	TCCRAC	2 cut(s)	1201, 1328
Mph1103I	ATGCAT	2 cut(s)	302, 384
MroI	TCCGGA	1 cut(s)	519
MseI	TTAA	5 cut(s)	501, 1206, 1463, 1566, 1967
MslI	CAYNNNNRTG	3 cut(s)	101, 441, 2298
MspA1I	CMGCKG	2 cut(s)	841, 2152
MspCI	CTTAAG	1 cut(s)	1565
MspI	CCGG	4 cut(s)	304, 520, 2088, 2094
MspR9I	CCNGG	4 cut(s)	1035, 1156, 2089, 2095
MssI	GTTTAAAC	1 cut(s)	1968
MunI	CAATTG	1 cut(s)	144
Mva1269I	GAATGC	1 cut(s)	2178
MvaI	CCWGG	2 cut(s)	1035, 1156
MwoI	GCNNNNNNNGC	8 cut(s)	375, 388, 644, 902, 1010, 1022, 1087, 1931
NciI	CCSGG	2 cut(s)	2089, 2095
NcoI	CCATGG	1 cut(s)	165
NdeI	CATATG	1 cut(s)	211
NdeII	GATC	3 cut(s)	457, 712, 1246
NlaIV	GGNNCC	8 cut(s)	12, 13, 1262, 1263, 2065, 2092, 2145, 2238
NmuCI	GTSAC	2 cut(s)	1494, 1896
NsiI	ATGCAT	2 cut(s)	302, 384
NspI	RCATGY	5 cut(s)	382, 386, 1108, 1718, 2297
PaeI	GCATGC	1 cut(s)	382
PaeR7I	CTCGAG	1 cut(s)	1091
PagI	TCATGA	4 cut(s)	157, 181, 1272, 1999
PciI	ACATGT	1 cut(s)	2293
PciSI	GCTCTTC	1 cut(s)	717
PctI	GAATGC	1 cut(s)	2178
PfeI	GAWTC	6 cut(s)	107, 176, 516, 1652, 2003, 2041
PflMI	CCANNNNNTGG	1 cut(s)	2281
PfoI	TCCNGGA	1 cut(s)	2093
PleI	GAGTC	3 cut(s)	77, 2068, 2201
PmeI	GTTTAAAC	1 cut(s)	1968
PpsI	GAGTC	3 cut(s)	77, 2068, 2201
PpuMI	RGGWCCY	1 cut(s)	11
PscI	ACATGT	1 cut(s)	2293
Psp1406I	AACGTT	1 cut(s)	1598
Psp5II	RGGWCCY	1 cut(s)	11
Psp6I	CCWGG	2 cut(s)	1033, 1154
PspFI	CCCAGC	1 cut(s)	1292
PspGI	CCWGG	2 cut(s)	1033, 1154
PspN4I	GGNNCC	8 cut(s)	12, 13, 1262, 1263, 2065, 2092, 2145, 2238
PspOMI	GGGCCC	1 cut(s)	1260
PspPI	GGNCC	8 cut(s)	11, 116, 433, 1260, 1261, 1872, 2091, 2214
PspPPI	RGGWCCY	1 cut(s)	11
PspXI	VCTCGAGB	1 cut(s)	1091
PstI	CTGCAG	1 cut(s)	544
PvuII	CAGCTG	1 cut(s)	2152
RsaI	GTAC	4 cut(s)	308, 1252, 1505, 2145
RsaNI	GTAC	4 cut(s)	307, 1251, 1504, 2144
RseI	CAYNNNNRTG	3 cut(s)	101, 441, 2298
SalI	GTCGAC	1 cut(s)	1866
SapI	GCTCTTC	1 cut(s)	717
SaqAI	TTAA	5 cut(s)	501, 1206, 1463, 1566, 1967
Sau3AI	GATC	3 cut(s)	457, 712, 1246
Sau96I	GGNCC	8 cut(s)	11, 116, 433, 1260, 1261, 1872, 2091, 2214
SchI	GAGTC	3 cut(s)	78, 2068, 2201
ScrFI	CCNGG	4 cut(s)	1035, 1156, 2089, 2095
SduI	GDGCHC	1 cut(s)	1264
SfcI	CTRYAG	3 cut(s)	540, 677, 1708
Sfr274I	CTCGAG	1 cut(s)	1091
SinI	GGWCC	4 cut(s)	11, 116, 433, 2091
SlaI	CTCGAG	1 cut(s)	1091
SmiMI	CAYNNNNRTG	3 cut(s)	101, 441, 2298
SmlI	CTYRAG	6 cut(s)	595, 1091, 1565, 1905, 1979, 2209
SmoI	CTYRAG	6 cut(s)	595, 1091, 1565, 1905, 1979, 2209
SpeI	ACTAGT	1 cut(s)	1498
SphI	GCATGC	1 cut(s)	382
SsiI	CCGC	8 cut(s)	60, 66, 189, 839, 1013, 1550, 2025, 2234
SspI	AATATT	2 cut(s)	665, 1881
SspMI	CTAG	5 cut(s)	261, 584, 1499, 2079, 2109
StyD4I	CCNGG	4 cut(s)	1033, 1154, 2087, 2093
StyI	CCWWGG	3 cut(s)	165, 270, 2078
TaaI	ACNGT	5 cut(s)	100, 916, 1314, 1639, 1976
TaiI	ACGT	2 cut(s)	1271, 1601
TaqI	TCGA	4 cut(s)	72, 346, 1092, 1867
TatI	WGTACW	1 cut(s)	1503
TauI	GCSGC	3 cut(s)	191, 1016, 1553
TfiI	GAWTC	6 cut(s)	107, 176, 516, 1652, 2003, 2041
Tru1I	TTAA	5 cut(s)	501, 1206, 1463, 1566, 1967
Tru9I	TTAA	5 cut(s)	501, 1206, 1463, 1566, 1967
TscAI	CASTG	6 cut(s)	241, 496, 549, 565, 1371, 1609
TseFI	GTSAC	2 cut(s)	1494, 1896
Tsp45I	GTSAC	2 cut(s)	1494, 1896
TspGWI	ACGGA	3 cut(s)	211, 324, 634
TspRI	CASTG	6 cut(s)	241, 496, 549, 565, 1371, 1609
Van91I	CCANNNNNTGG	1 cut(s)	2281
Vha464I	CTTAAG	1 cut(s)	1565
VpaK11BI	GGWCC	4 cut(s)	11, 116, 433, 2091
XapI	RAATTY	5 cut(s)	79, 290, 695, 1130, 1432
XbaI	TCTAGA	2 cut(s)	260, 583
XceI	RCATGY	5 cut(s)	382, 386, 1108, 1718, 2297
XcmI	CCANNNNNNNNNTGG	2 cut(s)	100, 405
XhoI	CTCGAG	1 cut(s)	1091
XmaJI	CCTAGG	1 cut(s)	2078
XmiI	GTMKAC	2 cut(s)	1711, 1867
XspI	CTAG	5 cut(s)	261, 584, 1499, 2079, 2109
Zsp2I	ATGCAT	2 cut(s)	302, 384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.