MD02G1000500.v1.1

Glucose-1-phosphate adenylyltransferase small subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016577
Physical Location & Seq
Forward (+)
12009 .. 14479
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1000500.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGGTTATCTTGCAGCGTTGTTTTCCCTCCGTTGTTAGCATCCGAGCAAAGTTCCCCGTTACTGCATCAGATGGTAGTATTCACGAAAACCGTTCCCTCAAGCTTCCTTCTACTACTCTTCCTCCGAGCTTCTTGATATCCAATTCACACCAGTCTCAGACTCCAACTTTGTTGTTTCCTCCAGCCAATCAGAGTGTGGCAGCTGTTGTGTTCGGGGATGGACACGAGTCACAACTTTACCCGCTGACAAAGAGAAGATCAGAAGGGGCGATACCTATTGCAGCAAATTACAGGCTCATTGATTCAGTTGTGAGCAACTGCATTAGCAGCAACATAAGCCATATATACGCTCTAACACAATTTAACTCTACTTCTCTCAATTCCCACCTTTCCAGAGCTTACTCTGGAGTTTGTCTTGGGAATGACGGCTTTGTTCAAGTCATTGCCGCGTATCAGAGCCCTGGCAATAACGGATGGTTTCAGGGAACTGCTGATGCTGTGAGAAGATGTCTGTGGATGCTGGAAGAGCATCCAATGACTGAGTTTTTGGTCCTTCCTGGTCACCATCTCTACAGAATGGACTACCAGAAACTCATCAAGGCCCACCGAGACAACGACGCTGACATTACAATTGCTGTATCTGTTGCCAGGAGACTTCATGATCCGGGATTTGGCTTTCTAAATGTGAATTCTCAAAATCAAGTTGTAGATTTCAGATTGAAGTTGGAGGGAAAGCCATTTAATGTTGCTTCAGCCAAAAGCTCAAGTAACTCCAACGATACTGCTCTAAGTAGCATGGCAAGTATGGGAATCTTTCTCATCAACAGAGGTGCAATGAAAAGGCTACTGGAAGAACATTTTCCCAAGGCAAATGACTTCACAAGTGAAGTAATTCCGGGTGCCATATCTATTGGAATGAAGGTTCAAGCTTATGCATTTGATGGATATTGGGAGGACCTGAGGAATATTAAAGCTTTCTACGAAGCAAATATGCAAAGCACAAAGGATGCAGATGTGGGATACAACTTCTACGACAGAGAGAGCCCAGTCTACACATTGCCTCGGTATCTGCCTCCAACTCAAATAACAGACTCTGAAATTACAAACAGTGTAATTGGAGATGGTTGCATCCTCAATGGATGCAGGATCAAAGGCACAGTACTTGGTATGAGGACGAGAATCGGAAATGGGGCTATAGTCGAGGATTCAATTATCATGGGTTCAACTATGTACCAAACAGAAGGGAAGAGGAAAGGCATGGCGGGTGCGATCCCTGGGATGGGAATTGGCGAAGATACTCAAGTAAGGAAGGCTATTATAGACAAGAATGCAAGAATTGGCAACAATGTTATGATCATTAATAAAGATAATGCGAAAGAAGCGGACAGAGAAGGTGATGGATATGTCATCCGTGACGGAATACTAGTCCTTCTTCCGAGTGCAGTGATTCCAGATGGCACCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko00520 ko01100 ko01110 ko02026 map00500 map00520 map01100 map01110 map02026
Pfam Domains
Protein Families
NTP_transferase

Protein Analysis

490

Amino Acids

53.56

Weight (kDa)

7.16

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 68 - 334 1.6e-40 Nucleotidyl transferase
LbH_GLGC PF25247 355 - 488 3.2e-47 GLGC left-handed parallel beta-helix domain
Hexapep_GlmU PF24894 364 - 408 1.9e-06 GlmU-like, C-terminal hexapeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05610 AT1G05610
malus_domestica MD02G1000500.v1.1 MD15G1142300.v1.1
prunus_persica Prupe.7G270900_v2.0.a1
pyrus_communis pycom15g12840 pycom290g00110 pycom290g00120
rosa_chinensis RchiOBHm_Chr2g0084401
rosa_multiflora Rmu_co8406699.1_g000001
rosa_roxburghii Rroxscaffold_2G00156130
rosa_samantha Rh2AG001500 Rh2CG001400
rosa_wichuraiana Rw2G000120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 899, 1457
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 1 cut(s) 449
AciI CCGC 4 cut(s) 242, 447, 1262, 1382
AclWI GGATC 3 cut(s) 656, 1154, 1264
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 1 cut(s) 735
AfaI GTAC 2 cut(s) 1161, 1232
AfiI CCNNNNNNNGG 3 cut(s) 671, 1279, 1280
AgsI TTSAA 5 cut(s) 437, 721, 926, 1209, 1224
AhlI ACTAGT 1 cut(s) 1423
AjnI CCWGG 4 cut(s) 460, 556, 647, 1273
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 7 cut(s) 103, 129, 203, 398, 762, 929, 974
AluI AGCT 7 cut(s) 103, 129, 203, 398, 762, 929, 974
Alw26I GTCTC 3 cut(s) 159, 603, 646
AlwI GGATC 3 cut(s) 656, 1154, 1264
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 1 cut(s) 600
ApeKI GCWGC 4 cut(s) 13, 200, 281, 327
ApoI RAATTY 1 cut(s) 688
ArsI GACNNNNNNTTYG 4 cut(s) 151, 183, 529, 561
AseI ATTAAT 1 cut(s) 1359
Asp700I GAANNNNTTC 2 cut(s) 858, 891
AspS9I GGNCC 3 cut(s) 550, 601, 955
AsuC2I CCSGG 2 cut(s) 666, 897
AsuHPI GGTGA 2 cut(s) 554, 1406
AvaII GGWCC 2 cut(s) 550, 955
AxyI CCTNAGG 1 cut(s) 959
BaeI ACNNNNGTAYC 2 cut(s) 1287, 1320
BanI GGYRCC 2 cut(s) 899, 1457
BanII GRGCYC 2 cut(s) 461, 1046
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
BauI CACGAG 1 cut(s) 224
BbvI GCAGC 4 cut(s) 25, 212, 293, 339
BccI CCATC 9 cut(s) 65, 212, 468, 573, 935, 1115, 1273, 1391, 1448
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 4 cut(s) 462, 558, 649, 1275
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 1 cut(s) 1353
BcnI CCSGG 2 cut(s) 666, 897
BcoDI GTCTC 3 cut(s) 159, 603, 646
BcuI ACTAGT 1 cut(s) 1423
BfaI CTAG 1 cut(s) 1424
BfmI CTRYAG 2 cut(s) 571, 1194
BfuI GTATCC 1 cut(s) 1013
BisI GCNGC 5 cut(s) 14, 201, 282, 328, 447
BlsI GCNGC 5 cut(s) 15, 202, 283, 329, 448
BmcAI AGTACT 1 cut(s) 1161
Bme1390I CCNGG 6 cut(s) 462, 558, 649, 666, 897, 1275
Bme18I GGWCC 2 cut(s) 550, 955
BmgT120I GGNCC 3 cut(s) 550, 601, 955
BmiI GGNNCC 2 cut(s) 901, 1459
BmrFI CCNGG 6 cut(s) 462, 558, 649, 666, 897, 1275
BmrI ACTGGG 1 cut(s) 1040
BmsI GCATC 8 cut(s) 48, 74, 484, 507, 538, 997, 1130, 1137
BmuI ACTGGG 1 cut(s) 1040
BoxI GACNNNNGTC 1 cut(s) 226
BpmI CTGGAG 2 cut(s) 165, 426
BpuEI CTTGAG 3 cut(s) 83, 748, 1284
BpuMI CCSGG 2 cut(s) 666, 897
BsaJI CCNNGG 5 cut(s) 460, 864, 1061, 1273, 1274
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 3 cut(s) 671, 1279, 1280
Bse1I ACTGG 3 cut(s) 151, 852, 1046
Bse21I CCTNAGG 1 cut(s) 959
Bse3DI GCAATG 3 cut(s) 441, 840, 1055
BseBI CCWGG 4 cut(s) 462, 558, 649, 1275
BseDI CCNNGG 5 cut(s) 460, 864, 1061, 1273, 1274
BseLI CCNNNNNNNGG 3 cut(s) 671, 1279, 1280
BseMI GCAATG 3 cut(s) 441, 840, 1055
BseMII CTCAG 3 cut(s) 170, 531, 950
BseNI ACTGG 3 cut(s) 151, 852, 1046
BseXI GCAGC 4 cut(s) 25, 212, 293, 339
BsgI GTGCAG 1 cut(s) 1461
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 1 cut(s) 602
BshNI GGYRCC 2 cut(s) 899, 1457
BsiSI CCGG 2 cut(s) 665, 896
BslI CCNNNNNNNGG 3 cut(s) 671, 1279, 1280
BsmAI GTCTC 3 cut(s) 159, 603, 646
BsmI GAATGC 1 cut(s) 1333
BsnI GGCC 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 461, 1046
Bsp143I GATC 5 cut(s) 257, 661, 1146, 1269, 1353
BspACI CCGC 4 cut(s) 242, 447, 1262, 1382
BspANI GGCC 1 cut(s) 602
BspCNI CTCAG 3 cut(s) 169, 532, 951
BspFNI CGCG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 901, 1459
BspPI GGATC 3 cut(s) 656, 1154, 1264
BspQI GCTCTTC 1 cut(s) 519
BspT107I GGYRCC 2 cut(s) 899, 1457
BsrDI GCAATG 3 cut(s) 441, 840, 1055
BsrI ACTGG 3 cut(s) 151, 852, 1046
BssECI CCNNGG 5 cut(s) 460, 864, 1061, 1273, 1274
BssMI GATC 5 cut(s) 257, 661, 1146, 1269, 1353
BssSI CACGAG 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 864
Bst2BI CACGAG 1 cut(s) 224
Bst2UI CCWGG 4 cut(s) 462, 558, 649, 1275
Bst4CI ACNGT 3 cut(s) 92, 1109, 1159
Bst6I CTCTTC 3 cut(s) 123, 519, 1241
BstDEI CTNAG 4 cut(s) 156, 540, 788, 959
BstEII GGTNACC 1 cut(s) 560
BstFNI CGCG 1 cut(s) 449
BstKTI GATC 5 cut(s) 260, 664, 1149, 1272, 1356
BstMAI GTCTC 3 cut(s) 159, 603, 646
BstMBI GATC 5 cut(s) 257, 661, 1146, 1269, 1353
BstMWI GCNNNNNNNGC 4 cut(s) 327, 336, 526, 1379
BstNI CCWGG 4 cut(s) 462, 558, 649, 1275
BstPAI GACNNNNGTC 1 cut(s) 226
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 6 cut(s) 460, 556, 647, 664, 895, 1273
BstSFI CTRYAG 2 cut(s) 571, 1194
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 4 cut(s) 25, 212, 293, 339
Bsu36I CCTNAGG 1 cut(s) 959
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 1 cut(s) 602
BtsI GCAGTG 1 cut(s) 1449
BtsIMutI CAGTG 2 cut(s) 1114, 1449
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 1 cut(s) 658
Cfr13I GGNCC 3 cut(s) 550, 601, 955
CseI GACGC 1 cut(s) 626
Csp6I GTAC 2 cut(s) 1160, 1231
CviAII CATG 4 cut(s) 659, 796, 1216, 1258
CviQI GTAC 2 cut(s) 1160, 1231
DdeI CTNAG 4 cut(s) 156, 540, 788, 959
DpnI GATC 5 cut(s) 259, 663, 1148, 1271, 1355
DpnII GATC 5 cut(s) 257, 661, 1146, 1269, 1353
Eam1104I CTCTTC 3 cut(s) 123, 519, 1241
EarI CTCTTC 3 cut(s) 123, 519, 1241
Eco130I CCWWGG 1 cut(s) 864
Eco24I GRGCYC 2 cut(s) 461, 1046
Eco32I GATATC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 550, 955
Eco57I CTGAAG 1 cut(s) 735
Eco81I CCTNAGG 1 cut(s) 959
Eco91I GGTNACC 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 955
EcoO65I GGTNACC 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 4 cut(s) 460, 556, 647, 1273
EcoRV GATATC 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 864
EcoT22I ATGCAT 1 cut(s) 937
EcoT38I GRGCYC 2 cut(s) 461, 1046
ErhI CCWWGG 1 cut(s) 864
FaeI CATG 4 cut(s) 662, 799, 1219, 1261
FatI CATG 4 cut(s) 658, 795, 1215, 1257
FauI CCCGC 2 cut(s) 249, 1255
FbaI TGATCA 1 cut(s) 1353
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 5 cut(s) 14, 201, 282, 328, 447
FriOI GRGCYC 2 cut(s) 461, 1046
Fsp4HI GCNGC 5 cut(s) 14, 201, 282, 328, 447
FspBI CTAG 1 cut(s) 1424
GluI GCNGC 5 cut(s) 14, 201, 282, 328, 447
GsuI CTGGAG 2 cut(s) 165, 426
HaeIII GGCC 1 cut(s) 602
HapII CCGG 2 cut(s) 665, 896
HgaI GACGC 1 cut(s) 626
Hin1II CATG 4 cut(s) 662, 799, 1219, 1261
HindIII AAGCTT 3 cut(s) 101, 927, 972
HinfI GANTC 8 cut(s) 160, 227, 302, 810, 1091, 1179, 1205, 1447
HpaII CCGG 2 cut(s) 665, 896
HphI GGTGA 2 cut(s) 554, 1406
Hpy166II GTNNAC 1 cut(s) 1051
Hpy188III TCNNGA 6 cut(s) 83, 133, 393, 405, 659, 1451
Hpy8I GTNNAC 1 cut(s) 1051
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 8 cut(s) 117, 257, 563, 913, 1235, 1303, 1385, 1439
HpyCH4III ACNGT 3 cut(s) 92, 1109, 1159
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 336, 526, 1379
HpyF3I CTNAG 4 cut(s) 156, 540, 788, 959
Hsp92II CATG 4 cut(s) 662, 799, 1219, 1261
Ksp22I TGATCA 1 cut(s) 1353
Kzo9I GATC 5 cut(s) 257, 661, 1146, 1269, 1353
LguI GCTCTTC 1 cut(s) 519
Lsp1109I GCAGC 4 cut(s) 25, 212, 293, 339
LweI GCATC 8 cut(s) 48, 74, 484, 507, 538, 997, 1130, 1137
MaeI CTAG 1 cut(s) 1424
MaeIII GTNAC 5 cut(s) 58, 228, 560, 767, 1412
MalI GATC 5 cut(s) 259, 663, 1148, 1271, 1355
MboI GATC 5 cut(s) 257, 661, 1146, 1269, 1353
MboII GAAGA 8 cut(s) 110, 267, 516, 536, 863, 1258, 1304, 1424
MfeI CAATTG 1 cut(s) 630
MhlI GDGCHC 2 cut(s) 461, 1046
MlyI GAGTC 3 cut(s) 154, 236, 1085
MmeI TCCRAC 4 cut(s) 188, 705, 798, 1100
Mph1103I ATGCAT 1 cut(s) 937
MroXI GAANNNNTTC 2 cut(s) 858, 891
MseI TTAA 4 cut(s) 363, 741, 969, 1359
MspA1I CMGCKG 2 cut(s) 203, 244
MspI CCGG 2 cut(s) 665, 896
MspR9I CCNGG 6 cut(s) 462, 558, 649, 666, 897, 1275
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 1333
MvaI CCWGG 4 cut(s) 462, 558, 649, 1275
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 4 cut(s) 327, 336, 526, 1379
NciI CCSGG 2 cut(s) 666, 897
NdeII GATC 5 cut(s) 257, 661, 1146, 1269, 1353
NlaIII CATG 4 cut(s) 662, 799, 1219, 1261
NlaIV GGNNCC 2 cut(s) 901, 1459
NmuCI GTSAC 3 cut(s) 228, 560, 1412
NsiI ATGCAT 1 cut(s) 937
PagI TCATGA 1 cut(s) 658
PasI CCCWGGG 1 cut(s) 1274
PciSI GCTCTTC 1 cut(s) 519
PctI GAATGC 1 cut(s) 1333
PdmI GAANNNNTTC 2 cut(s) 858, 891
PfeI GAWTC 5 cut(s) 302, 810, 1179, 1205, 1447
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 5 cut(s) 15, 202, 283, 329, 448
PleI GAGTC 3 cut(s) 154, 235, 1085
PpsI GAGTC 3 cut(s) 154, 235, 1085
PpuMI RGGWCCY 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 226
PshBI ATTAAT 1 cut(s) 1359
Psp5II RGGWCCY 1 cut(s) 955
Psp6I CCWGG 4 cut(s) 460, 556, 647, 1273
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 4 cut(s) 460, 556, 647, 1273
PspN4I GGNNCC 2 cut(s) 901, 1459
PspPI GGNCC 3 cut(s) 550, 601, 955
PspPPI RGGWCCY 1 cut(s) 955
PstNI CAGNNNCTG 1 cut(s) 1094
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 2 cut(s) 1161, 1232
RsaNI GTAC 2 cut(s) 1160, 1231
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 4 cut(s) 363, 741, 969, 1359
SatI GCNGC 5 cut(s) 14, 201, 282, 328, 447
Sau3AI GATC 5 cut(s) 257, 661, 1146, 1269, 1353
Sau96I GGNCC 3 cut(s) 550, 601, 955
ScaI AGTACT 1 cut(s) 1161
SchI GAGTC 3 cut(s) 154, 236, 1085
ScrFI CCNGG 6 cut(s) 462, 558, 649, 666, 897, 1275
SduI GDGCHC 2 cut(s) 461, 1046
SfaNI GCATC 8 cut(s) 48, 74, 484, 507, 538, 997, 1130, 1137
SfcI CTRYAG 2 cut(s) 571, 1194
SinI GGWCC 2 cut(s) 550, 955
SmlI CTYRAG 3 cut(s) 98, 763, 1299
SmoI CTYRAG 3 cut(s) 98, 763, 1299
SpeI ACTAGT 1 cut(s) 1423
SsiI CCGC 4 cut(s) 242, 447, 1262, 1382
SspI AATATT 1 cut(s) 967
SspMI CTAG 1 cut(s) 1424
StyD4I CCNGG 6 cut(s) 460, 556, 647, 664, 895, 1273
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 3 cut(s) 92, 1109, 1159
TaqI TCGA 1 cut(s) 1200
TatI WGTACW 1 cut(s) 1159
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 5 cut(s) 302, 810, 1179, 1205, 1447
Tru1I TTAA 4 cut(s) 363, 741, 969, 1359
Tru9I TTAA 4 cut(s) 363, 741, 969, 1359
TscAI CASTG 2 cut(s) 1114, 1449
TseFI GTSAC 3 cut(s) 228, 560, 1412
TseI GCWGC 4 cut(s) 13, 200, 281, 327
Tsp45I GTSAC 3 cut(s) 228, 560, 1412
TspDTI ATGAA 3 cut(s) 647, 851, 932
TspGWI ACGGA 4 cut(s) 19, 486, 1400, 1431
TspRI CASTG 2 cut(s) 1114, 1449
VpaK11BI GGWCC 2 cut(s) 550, 955
VspI ATTAAT 1 cut(s) 1359
XapI RAATTY 1 cut(s) 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 1050
XmnI GAANNNNTTC 2 cut(s) 858, 891
XspI CTAG 1 cut(s) 1424
ZrmI AGTACT 1 cut(s) 1161
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.