Rh2AG001500

Glucose-1-phosphate adenylyltransferase small subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016577
Physical Location & Seq
Reverse (-)
170357 .. 177542
7186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG001500.1

Sequence Viewer

Length: 1479 bp
ATGGTGATCTTGCAGGTTTGTCTTCCCCCCCTTGTTAGTAGTAGTACTCAAACAAAGCGTCCCATTATCGTTCGATCAGACTATTGTAATCATGAAAAACGTTCACTGAAGCTTCATTCTACTGTTCCTCCAAGCTTGTTGGTATCCAATTCACAGCAAACTCAAAATCCCGCTGTCTTGTTTCCCCCAGCTAATCAGAGTGTGGCAGCTATTGTGTTTGGGGATGGGCCAGAAACACAACTCTATCCATTGACAAAGAGAAGATCAGAAGGAGCCATACCTATCGCAGCAAGTTATAGACTGATTGATGCAGTTTTGAGCAACTGCATTAGCAGCAATATACACAGGATATATGCTCTGACACAATTTAACTCGACTTCTCTTAATTCCCACATTTCCAGGGCTTATTCTGGACTAGGTTTTGGAAATGAGGGGTTTGTTGAAGTAATTGCAGCATATCAAAGCTCTGATAATAATGGATGGTTTCAGGGAACTGCTGATGCTGTTAGAAGATGCCTATGGGTGCTGGAAGAGTATCCAATGTCTGAGTTTTTGGTCCTTCCTGGTTACCATCTCTACAAAATGGACTACCAGGAACTCATCAAGGCCCATCGAAACAATAAGGCAGACATCACAGTTGCAGCATCAGTTGCTAGGAGAATTCATGATCCGGGTTTTGGCTTTTTAGATGTGAATTCTGAAAATCAAGTTCTAGAGTTCAGATTGAAGTTGGAGGGAGGGCCAATTGATAATCAATCAGCCAAAGGCTCAAGGAAATCCAACGATACTGCTCTAAGTAGCATCACAAGTATGGGAATCTTTCTCATCAACAGAGATGCATTGAAAAGGCTCCTAGAAGAACATTTTCCCGAGGCAAATGACTTTAGAACTGAAGTGATTCCCGGTGCCATATCCATTGGAATGAAGATTCAAGCTTATGCATTTCATGGATACTGGGAGGACATGAGGAATATTGAAGCATTCTACCAAGCAAATATGCAAAGTACAAAGAGTACAGACGTGGGATATAACTTTTGTGATAAAGAAAGTCCAGTCCACACTTTGCGTCGTTGTCTGCCTCCAACTCTTATAACTGATTCTGTAATAACAAATAGTGTAGTTGGAGATGGTTGCATTCTTAATGGTTGCAGGATCAAAGGTACAGTAATTGGTATGAGGACAAGAATCGGAAATGGGTCAATAATCAAGGATTCAGTGATCATGGGTTCAACTTTATATCAACCAAACACAGAAGATAGTGAAAACAGGAAAGGCACAGACATTCCCATCGGAATTGGGGAAGATACTCAAATAAGGAAGGCTATTGTAGACAAGAATGCAAGGATTGGCAAGAATGTAGTGATCATTAACAAAGACAATGCCCATGAAGGGGACAGAGAAACCGATGGCTATGTCATTCGTCAGGGAATAGTAGTTGTTCTTCGAAGTGCTGTAATCCCAGATGGCACCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko00520 ko01100 ko01110 ko02026 map00500 map00520 map01100 map01110 map02026
Pfam Domains
Protein Families
NTP_transferase

Protein Analysis

492

Amino Acids

54.31

Weight (kDa)

7.16

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 70 - 337 1.3e-38 Nucleotidyl transferase
LbH_GLGC PF25247 357 - 491 3.3e-49 GLGC left-handed parallel beta-helix domain
Hexapep_GlmU PF24894 367 - 410 6.8e-06 GlmU-like, C-terminal hexapeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05610 AT1G05610
malus_domestica MD02G1000500.v1.1 MD15G1142300.v1.1
prunus_persica Prupe.7G270900_v2.0.a1
pyrus_communis pycom15g12840 pycom290g00110 pycom290g00120
rosa_chinensis RchiOBHm_Chr2g0084401
rosa_multiflora Rmu_co8406699.1_g000001
rosa_roxburghii Rroxscaffold_2G00156130
rosa_samantha Rh2AG001500 Rh2CG001400
rosa_wichuraiana Rw2G000120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
Acc36I ACCTGC 1 cut(s) 4
AccB1I GGYRCC 2 cut(s) 905, 1466
AccI GTMKAC 1 cut(s) 1329
AciI CCGC 1 cut(s) 171
AclI AACGTT 1 cut(s) 100
AclWI GGATC 2 cut(s) 662, 1160
AcsI RAATTY 2 cut(s) 660, 694
AcuI CTGAAG 2 cut(s) 128, 912
AfaI GTAC 4 cut(s) 46, 1006, 1015, 1162
AfiI CCNNNNNNNGG 3 cut(s) 677, 1389, 1390
AgsI TTSAA 6 cut(s) 443, 727, 844, 932, 977, 1230
AjiI CACGTC 1 cut(s) 1021
AjnI CCWGG 3 cut(s) 398, 562, 591
AluBI AGCT 6 cut(s) 112, 135, 191, 209, 465, 935
AluI AGCT 6 cut(s) 112, 135, 191, 209, 465, 935
AlwI GGATC 2 cut(s) 662, 1160
Ama87I CYCGRG 1 cut(s) 869
AoxI GGCC 3 cut(s) 227, 606, 740
ApeKI GCWGC 5 cut(s) 206, 287, 333, 452, 641
ApoI RAATTY 2 cut(s) 660, 694
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
Asp700I GAANNNNTTC 2 cut(s) 864, 897
AspS9I GGNCC 4 cut(s) 227, 556, 607, 740
AsuC2I CCSGG 2 cut(s) 672, 903
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 1444
AvaI CYCGRG 1 cut(s) 869
AvaII GGWCC 1 cut(s) 556
BanI GGYRCC 2 cut(s) 905, 1466
BarI GAAGNNNNNNTAC 4 cut(s) 261, 293, 1425, 1457
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 5 cut(s) 218, 299, 345, 464, 653
BccI CCATC 8 cut(s) 218, 474, 579, 618, 1121, 1295, 1400, 1457
BciT130I CCWGG 3 cut(s) 400, 564, 593
BciVI GTATCC 3 cut(s) 154, 546, 944
BclI TGATCA 2 cut(s) 1218, 1362
BcnI CCSGG 2 cut(s) 672, 903
BfaI CTAG 4 cut(s) 416, 654, 713, 854
BfuAI ACCTGC 1 cut(s) 4
BfuI GTATCC 3 cut(s) 154, 546, 944
BisI GCNGC 5 cut(s) 207, 288, 334, 453, 642
BlsI GCNGC 5 cut(s) 208, 289, 335, 454, 643
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 5 cut(s) 400, 564, 593, 672, 903
Bme18I GGWCC 1 cut(s) 556
BmeT110I CYCGRG 1 cut(s) 869
BmgBI CACGTC 1 cut(s) 1021
BmgT120I GGNCC 4 cut(s) 227, 556, 607, 740
BmiI GGNNCC 4 cut(s) 274, 851, 907, 1468
BmrFI CCNGG 5 cut(s) 400, 564, 593, 672, 903
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 6 cut(s) 298, 490, 503, 653, 810, 826
BmuI ACTGGG 1 cut(s) 964
BpiI GAAGAC 1 cut(s) 14
Bpu14I TTCGAA 1 cut(s) 1444
BpuEI CTTGAG 1 cut(s) 754
BpuMI CCSGG 2 cut(s) 672, 903
BsaJI CCNNGG 2 cut(s) 399, 870
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 3 cut(s) 677, 1389, 1390
Bse1I ACTGG 2 cut(s) 959, 1052
BseBI CCWGG 3 cut(s) 400, 564, 593
BseDI CCNNGG 2 cut(s) 399, 870
BseGI GGATG 2 cut(s) 229, 485
BseLI CCNNNNNNNGG 3 cut(s) 677, 1389, 1390
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 2 cut(s) 959, 1052
BseXI GCAGC 5 cut(s) 218, 299, 345, 464, 653
BseYI CCCAGC 1 cut(s) 187
BshFI GGCC 3 cut(s) 229, 608, 742
BshNI GGYRCC 2 cut(s) 905, 1466
BsiHKCI CYCGRG 1 cut(s) 869
BsiSI CCGG 2 cut(s) 671, 903
BslFI GGGAC 2 cut(s) 45, 1406
BslI CCNNNNNNNGG 3 cut(s) 677, 1389, 1390
BsmFI GGGAC 2 cut(s) 45, 1406
BsmI GAATGC 3 cut(s) 980, 1134, 1342
BsnI GGCC 3 cut(s) 229, 608, 742
BsoBI CYCGRG 1 cut(s) 869
Bsp119I TTCGAA 1 cut(s) 1444
Bsp143I GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
BspACI CCGC 1 cut(s) 171
BspANI GGCC 3 cut(s) 229, 608, 742
BspCNI CTCAG 1 cut(s) 538
BspHI TCATGA 2 cut(s) 91, 664
BspLI GGNNCC 4 cut(s) 274, 851, 907, 1468
BspMI ACCTGC 1 cut(s) 4
BspPI GGATC 2 cut(s) 662, 1160
BspT104I TTCGAA 1 cut(s) 1444
BspT107I GGYRCC 2 cut(s) 905, 1466
BsrI ACTGG 2 cut(s) 959, 1052
BssECI CCNNGG 2 cut(s) 399, 870
BssMI GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
Bst2UI CCWGG 3 cut(s) 400, 564, 593
Bst4CI ACNGT 3 cut(s) 124, 637, 1165
Bst6I CTCTTC 1 cut(s) 525
BstAPI GCANNNNNTGC 1 cut(s) 650
BstBI TTCGAA 1 cut(s) 1444
BstDEI CTNAG 2 cut(s) 546, 794
BstEII GGTNACC 1 cut(s) 566
BstF5I GGATG 2 cut(s) 229, 485
BstKTI GATC 7 cut(s) 9, 77, 266, 670, 1155, 1221, 1365
BstMBI GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
BstMWI GCNNNNNNNGC 2 cut(s) 333, 650
BstNI CCWGG 3 cut(s) 400, 564, 593
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 5 cut(s) 398, 562, 591, 670, 901
BstV1I GCAGC 5 cut(s) 218, 299, 345, 464, 653
BstV2I GAAGAC 1 cut(s) 14
BsuI GTATCC 3 cut(s) 154, 546, 944
BsuRI GGCC 3 cut(s) 229, 608, 742
BtrI CACGTC 1 cut(s) 1021
BtsCI GGATG 2 cut(s) 229, 485
BtsIMutI CAGTG 2 cut(s) 104, 1221
BveI ACCTGC 1 cut(s) 4
CciI TCATGA 2 cut(s) 91, 664
Cfr13I GGNCC 4 cut(s) 227, 556, 607, 740
CseI GACGC 2 cut(s) 47, 1055
Csp6I GTAC 4 cut(s) 45, 1005, 1014, 1161
CviAII CATG 6 cut(s) 92, 665, 947, 964, 1222, 1385
CviQI GTAC 4 cut(s) 45, 1005, 1014, 1161
DdeI CTNAG 2 cut(s) 546, 794
DpnI GATC 7 cut(s) 8, 76, 265, 669, 1154, 1220, 1364
DpnII GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 556
Eco57I CTGAAG 2 cut(s) 128, 912
Eco88I CYCGRG 1 cut(s) 869
Eco91I GGTNACC 1 cut(s) 566
EcoO65I GGTNACC 1 cut(s) 566
EcoRI GAATTC 2 cut(s) 660, 694
EcoRII CCWGG 3 cut(s) 398, 562, 591
EcoT22I ATGCAT 2 cut(s) 841, 943
FaeI CATG 6 cut(s) 95, 668, 950, 967, 1225, 1388
FaqI GGGAC 2 cut(s) 45, 1406
FatI CATG 6 cut(s) 91, 664, 946, 963, 1221, 1384
FauI CCCGC 1 cut(s) 178
FbaI TGATCA 2 cut(s) 1218, 1362
FblI GTMKAC 1 cut(s) 1329
Fnu4HI GCNGC 5 cut(s) 207, 288, 334, 453, 642
FokI GGATG 2 cut(s) 236, 492
Fsp4HI GCNGC 5 cut(s) 207, 288, 334, 453, 642
FspBI CTAG 4 cut(s) 416, 654, 713, 854
GluI GCNGC 5 cut(s) 207, 288, 334, 453, 642
GsaI CCCAGC 1 cut(s) 191
HaeIII GGCC 3 cut(s) 229, 608, 742
HapII CCGG 2 cut(s) 671, 903
HgaI GACGC 2 cut(s) 47, 1055
Hin1II CATG 6 cut(s) 95, 668, 950, 967, 1225, 1388
HindIII AAGCTT 3 cut(s) 110, 133, 933
HinfI GANTC 6 cut(s) 816, 898, 928, 1097, 1185, 1211
HpaII CCGG 2 cut(s) 671, 903
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 104, 1057, 1330
Hpy188III TCNNGA 5 cut(s) 92, 411, 665, 713, 869
Hpy8I GTNNAC 3 cut(s) 104, 1057, 1330
Hpy99I CGWCG 1 cut(s) 1071
HpyAV CCTTC 4 cut(s) 263, 569, 1312, 1382
HpyCH4III ACNGT 3 cut(s) 124, 637, 1165
HpyCH4IV ACGT 2 cut(s) 100, 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 650
HpyF3I CTNAG 2 cut(s) 546, 794
HpySE526I ACGT 2 cut(s) 100, 1020
Hsp92II CATG 6 cut(s) 95, 668, 950, 967, 1225, 1388
Ksp22I TGATCA 2 cut(s) 1218, 1362
Kzo9I GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
LmnI GCTCC 2 cut(s) 272, 855
Lsp1109I GCAGC 5 cut(s) 218, 299, 345, 464, 653
LweI GCATC 6 cut(s) 298, 490, 503, 653, 810, 826
MaeI CTAG 4 cut(s) 416, 654, 713, 854
MaeII ACGT 2 cut(s) 100, 1020
MaeIII GTNAC 1 cut(s) 566
MalI GATC 7 cut(s) 8, 76, 265, 669, 1154, 1220, 1364
MboI GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
MboII GAAGA 9 cut(s) 14, 273, 522, 542, 869, 937, 1265, 1313, 1433
MfeI CAATTG 1 cut(s) 744
MluCI AATT 9 cut(s) 148, 365, 385, 447, 660, 694, 744, 1167, 1293
MmeI TCCRAC 4 cut(s) 711, 804, 1102, 1106
MnlI CCTC 9 cut(s) 138, 424, 727, 731, 865, 952, 960, 1089, 1170
Mph1103I ATGCAT 2 cut(s) 841, 943
MroXI GAANNNNTTC 2 cut(s) 864, 897
MseI TTAA 4 cut(s) 369, 384, 1140, 1368
MslI CAYNNNNRTG 2 cut(s) 809, 920
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 671, 903
MspR9I CCNGG 5 cut(s) 400, 564, 593, 672, 903
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 3 cut(s) 980, 1134, 1342
MvaI CCWGG 3 cut(s) 400, 564, 593
MwoI GCNNNNNNNGC 2 cut(s) 333, 650
NciI CCSGG 2 cut(s) 672, 903
NdeII GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
NlaIII CATG 6 cut(s) 95, 668, 950, 967, 1225, 1388
NlaIV GGNNCC 4 cut(s) 274, 851, 907, 1468
NsiI ATGCAT 2 cut(s) 841, 943
NspV TTCGAA 1 cut(s) 1444
PagI TCATGA 2 cut(s) 91, 664
PctI GAATGC 3 cut(s) 980, 1134, 1342
PdmI GAANNNNTTC 2 cut(s) 864, 897
PfeI GAWTC 6 cut(s) 816, 898, 928, 1097, 1185, 1211
PkrI GCNGC 5 cut(s) 208, 289, 335, 454, 643
PsiI TTATAA 1 cut(s) 1091
Psp1406I AACGTT 1 cut(s) 100
Psp6I CCWGG 3 cut(s) 398, 562, 591
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 3 cut(s) 398, 562, 591
PspN4I GGNNCC 4 cut(s) 274, 851, 907, 1468
PspPI GGNCC 4 cut(s) 227, 556, 607, 740
RsaI GTAC 4 cut(s) 46, 1006, 1015, 1162
RsaNI GTAC 4 cut(s) 45, 1005, 1014, 1161
RseI CAYNNNNRTG 2 cut(s) 809, 920
SaqAI TTAA 4 cut(s) 369, 384, 1140, 1368
SatI GCNGC 5 cut(s) 207, 288, 334, 453, 642
Sau3AI GATC 7 cut(s) 6, 74, 263, 667, 1152, 1218, 1362
Sau96I GGNCC 4 cut(s) 227, 556, 607, 740
ScaI AGTACT 1 cut(s) 46
ScrFI CCNGG 5 cut(s) 400, 564, 593, 672, 903
SfaNI GCATC 6 cut(s) 298, 490, 503, 653, 810, 826
SfuI TTCGAA 1 cut(s) 1444
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 2 cut(s) 809, 920
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
Sse9I AATT 9 cut(s) 148, 365, 385, 447, 660, 694, 744, 1167, 1293
SsiI CCGC 1 cut(s) 171
SspI AATATT 1 cut(s) 973
SspMI CTAG 4 cut(s) 416, 654, 713, 854
StyD4I CCNGG 5 cut(s) 398, 562, 591, 670, 901
TaaI ACNGT 3 cut(s) 124, 637, 1165
TaiI ACGT 2 cut(s) 103, 1023
TaqI TCGA 4 cut(s) 73, 374, 613, 1444
TasI AATT 9 cut(s) 148, 365, 385, 447, 660, 694, 744, 1167, 1293
TatI WGTACW 3 cut(s) 44, 1004, 1013
TfiI GAWTC 6 cut(s) 816, 898, 928, 1097, 1185, 1211
Tru1I TTAA 4 cut(s) 369, 384, 1140, 1368
Tru9I TTAA 4 cut(s) 369, 384, 1140, 1368
TscAI CASTG 2 cut(s) 111, 1221
TseI GCWGC 5 cut(s) 206, 287, 333, 452, 641
TspDTI ATGAA 6 cut(s) 104, 108, 653, 935, 938, 1401
TspRI CASTG 2 cut(s) 111, 1221
VpaK11BI GGWCC 1 cut(s) 556
XapI RAATTY 2 cut(s) 660, 694
XbaI TCTAGA 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 1329
XmnI GAANNNNTTC 2 cut(s) 864, 897
XspI CTAG 4 cut(s) 416, 654, 713, 854
ZrmI AGTACT 1 cut(s) 46
Zsp2I ATGCAT 2 cut(s) 841, 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.