MD15G1142300.v1.1

Glucose-1-phosphate adenylyltransferase small subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016577
Physical Location & Seq
Reverse (-)
10531522 .. 10534673
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1142300.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGGTGATCTTGCAGCGTTGTTTTCCCTCCGTTGTTAGCATCCGAGCAAGGTGCTCCGTTACTGCATCAGATGGTAGTATTCACGAAAACCGTTCCCTCAAGCTTCATTCTACCACTCTTCCTCCGATGTTCTTGGTATCCAAGTCACACCAGTCTCAGACTCCAACTTTGTTGTTTCCTCCGGCCAATCAGAGTGTGGCAGCTGTTGTGTTCGGGGATGGGCACGAGTCTCAACTTTACCCGCTGACAAAGAGAAGATCAGAAGGGGCGATACCTATTGCAGCAAATTACAGGCTCATTGATTCGGTTGTGAGCAACTGCATTAGCAGCAACATAAGCCACATATATGCTCTCACACAGGTTAACTCTACTTCTCTCAATTCCCACCTTTCCAGAGCTTACTCTGGAGTTGGCCTTGGGAATGAGGGCTTTGTTCAGCTCATTGCCGCGTATCAGAGCCCCGGCAATAACACATGGTTTCAGGGAACTGCTGATGCTGTGAGAAGATGTCTGTGGGTGCTGGAAGAGTATCCCGTGACTGAGTTTTTGGTCCTTCCCGGTCACCATCTCTACAGTATGGACTACCAGAAACTCATCATGTCGCACCGAGACAATGGCGCTGACATTACCATTGCTACATCTGTTGCCAGGAGACTTCATGATCCGGGATTTGGCTTTCTAAATGTGAACTCTGAAAATCAAGTTACAGATTTCAGACTGAATTTGGAGGGAAAGTCAGTTGTGGTTGCTTCACCCAAAAGCTCAAGAAAATCCAACGATACTGCCCAAAGTAGTATGGCAAGTATGGGAATCTATCTCATCAACAGAGGTGTAATGAAAAGGCTTCTGGAAGAACATTTTCCAAAGGGAAATGACTTCACAAGTGAAGTAATTCCGGGTGCCATATCTTTCGGAATGAAGGTTCAAGCTTATGCATTTGATGGATATTGGGAGGACATGAGGAATATTAAAGCATTCTACGAAGCAAATATGCAAAGCACAAGGGATGCAGACGTAGGATATAACTTCTACGACAGAGAAAGTCCGGTCTACACACTGCCTCGGCGTCTGCCTCCAACTCAAATAGCAAACGCTGTAATTACAAACAGTGTAATTGGAGATGGTTGCATCCTCAATGGATGCAGGATCAAAGGGGCAGTAATTGGTATGAGGACGAGAATCGGAAATGGGGCTATAGTCGAGGATTCAGTCATCATGGGTTCAACCACGTACCAAACAGAAGATGGGGGGAAGAGGAAAGGCATGGCGGGTGCGATCCCCGTGATGGGAATTGGCGAAGATACTCAAGTAAGGAAGGCTATTATCGACAAGAATGCAAGAATTGGCAACAATGTTATGATCATCAATAAAGATAATGTGAAAGAAGCGGACAGAGAAGGTGATGGATATGTTATTCGTGACGGAATACTAGTCCTCCTTCCGAGTGCAGTAATTCCAGATGGCACCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko00520 ko01100 ko01110 ko02026 map00500 map00520 map01100 map01110 map02026
Pfam Domains
Protein Families
NTP_transferase

Protein Analysis

492

Amino Acids

53.69

Weight (kDa)

8.12

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 68 - 334 1.1e-38 Nucleotidyl transferase
LbH_GLGC PF25247 355 - 490 2.2e-46 GLGC left-handed parallel beta-helix domain
Hexapep_GlmU PF24894 364 - 409 2.5e-06 GlmU-like, C-terminal hexapeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05610 AT1G05610
malus_domestica MD02G1000500.v1.1 MD15G1142300.v1.1
prunus_persica Prupe.7G270900_v2.0.a1
pyrus_communis pycom15g12840 pycom290g00110 pycom290g00120
rosa_chinensis RchiOBHm_Chr2g0084401
rosa_multiflora Rmu_co8406699.1_g000001
rosa_roxburghii Rroxscaffold_2G00156130
rosa_samantha Rh2AG001500 Rh2CG001400
rosa_wichuraiana Rw2G000120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 899, 1463
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 1 cut(s) 449
AciI CCGC 4 cut(s) 242, 447, 1268, 1388
AclWI GGATC 3 cut(s) 656, 1154, 1270
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 721
AcyI GRCGYC 1 cut(s) 1066
AfaI GTAC 1 cut(s) 1232
AfiI CCNNNNNNNGG 3 cut(s) 671, 1285, 1286
AgsI TTSAA 2 cut(s) 926, 1224
AhlI ACTAGT 1 cut(s) 1429
AjnI CCWGG 1 cut(s) 647
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 6 cut(s) 103, 203, 398, 439, 762, 929
AluI AGCT 6 cut(s) 103, 203, 398, 439, 762, 929
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 4 cut(s) 159, 234, 603, 646
AlwI GGATC 3 cut(s) 656, 1154, 1270
AoxI GGCC 2 cut(s) 183, 412
ApeKI GCWGC 4 cut(s) 13, 200, 281, 327
ApoI RAATTY 1 cut(s) 721
ArsI GACNNNNNNTTYG 4 cut(s) 151, 183, 529, 561
Asp700I GAANNNNTTC 2 cut(s) 858, 891
AspLEI GCGC 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 550
AsuC2I CCSGG 4 cut(s) 462, 558, 666, 897
AsuHPI GGTGA 4 cut(s) 16, 554, 744, 1412
AvaII GGWCC 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 225
BaeI ACNNNNGTAYC 2 cut(s) 1293, 1326
BanI GGYRCC 2 cut(s) 899, 1463
BanII GRGCYC 1 cut(s) 461
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
BauI CACGAG 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 4 cut(s) 25, 212, 293, 339
BccI CCATC 9 cut(s) 65, 212, 573, 935, 1115, 1238, 1279, 1397, 1454
BcgI CGANNNNNNTGC 4 cut(s) 33, 67, 1316, 1350
BciT130I CCWGG 1 cut(s) 649
BciVI GTATCC 2 cut(s) 148, 540
BclI TGATCA 1 cut(s) 1359
BcnI CCSGG 4 cut(s) 462, 558, 666, 897
BcoDI GTCTC 4 cut(s) 159, 234, 603, 646
BcuI ACTAGT 1 cut(s) 1429
BfaI CTAG 1 cut(s) 1430
BfmI CTRYAG 2 cut(s) 571, 1194
BfoI RGCGCY 1 cut(s) 621
BfuI GTATCC 2 cut(s) 148, 540
BisI GCNGC 5 cut(s) 14, 201, 282, 328, 447
BlsI GCNGC 5 cut(s) 15, 202, 283, 329, 448
Bme1390I CCNGG 5 cut(s) 462, 558, 649, 666, 897
Bme18I GGWCC 1 cut(s) 550
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 2 cut(s) 901, 1465
BmrFI CCNGG 5 cut(s) 462, 558, 649, 666, 897
BmsI GCATC 6 cut(s) 48, 74, 484, 997, 1130, 1137
BpmI CTGGAG 1 cut(s) 426
BpuEI CTTGAG 3 cut(s) 83, 748, 1290
BpuMI CCSGG 4 cut(s) 462, 558, 666, 897
BsaAI YACGTR 1 cut(s) 1230
BsaHI GRCGYC 1 cut(s) 1066
BsaJI CCNNGG 3 cut(s) 415, 460, 1061
BsaWI WCCGGW 1 cut(s) 1045
BsaXI ACNNNNNCTCC 6 cut(s) 106, 136, 719, 749, 1419, 1449
Bsc4I CCNNNNNNNGG 3 cut(s) 671, 1285, 1286
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 2 cut(s) 441, 630
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 3 cut(s) 415, 460, 1061
BseGI GGATG 5 cut(s) 39, 223, 1012, 1128, 1145
BseLI CCNNNNNNNGG 3 cut(s) 671, 1285, 1286
BseMI GCAATG 2 cut(s) 441, 630
BseMII CTCAG 2 cut(s) 170, 531
BseNI ACTGG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 4 cut(s) 25, 212, 293, 339
BsgI GTGCAG 1 cut(s) 1467
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 2 cut(s) 185, 414
BshNI GGYRCC 2 cut(s) 899, 1463
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 6 cut(s) 182, 462, 558, 665, 896, 1046
BslI CCNNNNNNNGG 3 cut(s) 671, 1285, 1286
BsmAI GTCTC 4 cut(s) 159, 234, 603, 646
BsmI GAATGC 2 cut(s) 974, 1339
BsnI GGCC 2 cut(s) 185, 414
Bsp1286I GDGCHC 3 cut(s) 56, 225, 461
Bsp143I GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
BspACI CCGC 4 cut(s) 242, 447, 1268, 1388
BspANI GGCC 2 cut(s) 185, 414
BspCNI CTCAG 2 cut(s) 169, 532
BspFNI CGCG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 901, 1465
BspPI GGATC 3 cut(s) 656, 1154, 1270
BspT107I GGYRCC 2 cut(s) 899, 1463
BsrDI GCAATG 2 cut(s) 441, 630
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 3 cut(s) 415, 460, 1061
BssMI GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
BssNI GRCGYC 1 cut(s) 1066
BssSI CACGAG 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 415
Bst2BI CACGAG 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 3 cut(s) 92, 575, 1109
Bst6I CTCTTC 3 cut(s) 123, 519, 1247
BstACI GRCGYC 1 cut(s) 1066
BstBAI YACGTR 1 cut(s) 1230
BstDEI CTNAG 2 cut(s) 156, 540
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 5 cut(s) 39, 223, 1012, 1128, 1145
BstFNI CGCG 1 cut(s) 449
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 1 cut(s) 620
BstKTI GATC 6 cut(s) 9, 260, 664, 1149, 1278, 1362
BstMAI GTCTC 4 cut(s) 159, 234, 603, 646
BstMBI GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
BstMWI GCNNNNNNNGC 2 cut(s) 327, 336
BstNI CCWGG 1 cut(s) 649
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 5 cut(s) 460, 556, 647, 664, 895
BstSFI CTRYAG 2 cut(s) 571, 1194
BstSLI GKGCMC 1 cut(s) 225
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 4 cut(s) 25, 212, 293, 339
BsuI GTATCC 2 cut(s) 148, 540
BsuRI GGCC 2 cut(s) 185, 414
BtsCI GGATG 5 cut(s) 39, 223, 1012, 1128, 1145
BtsI GCAGTG 1 cut(s) 1055
BtsIMutI CAGTG 2 cut(s) 1055, 1114
CciI TCATGA 1 cut(s) 658
CfoI GCGC 1 cut(s) 620
Cfr13I GGNCC 1 cut(s) 550
CseI GACGC 1 cut(s) 1055
Csp6I GTAC 1 cut(s) 1231
CviAII CATG 6 cut(s) 474, 598, 659, 958, 1216, 1264
CviQI GTAC 1 cut(s) 1231
DdeI CTNAG 2 cut(s) 156, 540
DpnI GATC 6 cut(s) 8, 259, 663, 1148, 1277, 1361
DpnII GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 3 cut(s) 123, 519, 1247
EarI CTCTTC 3 cut(s) 123, 519, 1247
Eco130I CCWWGG 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 550
Eco91I GGTNACC 1 cut(s) 560
EcoO65I GGTNACC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 415
EcoT22I ATGCAT 1 cut(s) 937
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 6 cut(s) 477, 601, 662, 961, 1219, 1267
FatI CATG 6 cut(s) 473, 597, 658, 957, 1215, 1263
FauI CCCGC 2 cut(s) 249, 1261
FbaI TGATCA 1 cut(s) 1359
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 5 cut(s) 14, 201, 282, 328, 447
FokI GGATG 5 cut(s) 26, 230, 1019, 1115, 1152
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 5 cut(s) 14, 201, 282, 328, 447
FspBI CTAG 1 cut(s) 1430
GlaI GCGC 1 cut(s) 619
GluI GCNGC 5 cut(s) 14, 201, 282, 328, 447
GsuI CTGGAG 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 2 cut(s) 185, 414
HapII CCGG 6 cut(s) 182, 462, 558, 665, 896, 1046
HgaI GACGC 1 cut(s) 1055
HhaI GCGC 1 cut(s) 620
Hin1I GRCGYC 1 cut(s) 1066
Hin1II CATG 6 cut(s) 477, 601, 662, 961, 1219, 1267
Hin6I GCGC 1 cut(s) 618
HinP1I GCGC 1 cut(s) 618
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 101, 927
HinfI GANTC 6 cut(s) 160, 227, 302, 810, 1179, 1205
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 6 cut(s) 182, 462, 558, 665, 896, 1046
HphI GGTGA 4 cut(s) 16, 554, 744, 1412
Hpy166II GTNNAC 3 cut(s) 364, 688, 1051
Hpy188III TCNNGA 8 cut(s) 83, 393, 405, 659, 765, 848, 1418, 1457
Hpy8I GTNNAC 3 cut(s) 364, 688, 1051
HpyAV CCTTC 6 cut(s) 257, 563, 913, 1309, 1391, 1448
HpyCH4III ACNGT 3 cut(s) 92, 575, 1109
HpyCH4IV ACGT 2 cut(s) 1014, 1229
HpyF10VI GCNNNNNNNGC 2 cut(s) 327, 336
HpyF3I CTNAG 2 cut(s) 156, 540
HpySE526I ACGT 2 cut(s) 1014, 1229
Hsp92I GRCGYC 1 cut(s) 1066
Hsp92II CATG 6 cut(s) 477, 601, 662, 961, 1219, 1267
HspAI GCGC 1 cut(s) 618
Ksp22I TGATCA 1 cut(s) 1359
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 4 cut(s) 25, 212, 293, 339
LweI GCATC 6 cut(s) 48, 74, 484, 997, 1130, 1137
MaeI CTAG 1 cut(s) 1430
MaeII ACGT 2 cut(s) 1014, 1229
MaeIII GTNAC 6 cut(s) 58, 144, 535, 560, 703, 1418
MalI GATC 6 cut(s) 8, 259, 663, 1148, 1277, 1361
MboI GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
MboII GAAGA 8 cut(s) 110, 267, 516, 536, 863, 1253, 1264, 1310
MhlI GDGCHC 3 cut(s) 56, 225, 461
MlyI GAGTC 2 cut(s) 154, 236
MmeI TCCRAC 3 cut(s) 188, 798, 1100
Mph1103I ATGCAT 1 cut(s) 937
MroXI GAANNNNTTC 2 cut(s) 858, 891
MseI TTAA 2 cut(s) 363, 969
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 2 cut(s) 203, 244
MspI CCGG 6 cut(s) 182, 462, 558, 665, 896, 1046
MspR9I CCNGG 5 cut(s) 462, 558, 649, 666, 897
Mva1269I GAATGC 2 cut(s) 974, 1339
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 327, 336
NciI CCSGG 4 cut(s) 462, 558, 666, 897
NdeII GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
NlaIII CATG 6 cut(s) 477, 601, 662, 961, 1219, 1267
NlaIV GGNNCC 2 cut(s) 901, 1465
NmeAIII GCCGAG 1 cut(s) 1042
NmuCI GTSAC 4 cut(s) 144, 535, 560, 1418
NsiI ATGCAT 1 cut(s) 937
PagI TCATGA 1 cut(s) 658
PctI GAATGC 2 cut(s) 974, 1339
PdmI GAANNNNTTC 2 cut(s) 858, 891
PfeI GAWTC 4 cut(s) 302, 810, 1179, 1205
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 5 cut(s) 15, 202, 283, 329, 448
PleI GAGTC 2 cut(s) 154, 235
PpsI GAGTC 2 cut(s) 154, 235
Ppu21I YACGTR 1 cut(s) 1230
Psp6I CCWGG 1 cut(s) 647
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 2 cut(s) 901, 1465
PspPI GGNCC 1 cut(s) 550
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 1232
RsaNI GTAC 1 cut(s) 1231
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 363, 969
SatI GCNGC 5 cut(s) 14, 201, 282, 328, 447
Sau3AI GATC 6 cut(s) 6, 257, 661, 1146, 1275, 1359
Sau96I GGNCC 1 cut(s) 550
SchI GAGTC 2 cut(s) 154, 236
ScrFI CCNGG 5 cut(s) 462, 558, 649, 666, 897
SduI GDGCHC 3 cut(s) 56, 225, 461
SfaNI GCATC 6 cut(s) 48, 74, 484, 997, 1130, 1137
SfcI CTRYAG 2 cut(s) 571, 1194
SinI GGWCC 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 3 cut(s) 98, 763, 1305
SmoI CTYRAG 3 cut(s) 98, 763, 1305
SpeI ACTAGT 1 cut(s) 1429
SsiI CCGC 4 cut(s) 242, 447, 1268, 1388
SspI AATATT 1 cut(s) 967
SspMI CTAG 1 cut(s) 1430
StyD4I CCNGG 5 cut(s) 460, 556, 647, 664, 895
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 3 cut(s) 92, 575, 1109
TaiI ACGT 2 cut(s) 1017, 1232
TaqI TCGA 2 cut(s) 1200, 1326
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 4 cut(s) 302, 810, 1179, 1205
Tru1I TTAA 2 cut(s) 363, 969
Tru9I TTAA 2 cut(s) 363, 969
TscAI CASTG 2 cut(s) 1062, 1114
TseFI GTSAC 4 cut(s) 144, 535, 560, 1418
TseI GCWGC 4 cut(s) 13, 200, 281, 327
Tsp45I GTSAC 4 cut(s) 144, 535, 560, 1418
TspDTI ATGAA 4 cut(s) 95, 647, 851, 932
TspGWI ACGGA 3 cut(s) 19, 46, 1437
TspRI CASTG 2 cut(s) 1062, 1114
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 1 cut(s) 721
XcmI CCANNNNNNNNNTGG 2 cut(s) 193, 1241
XmiI GTMKAC 1 cut(s) 1050
XmnI GAANNNNTTC 2 cut(s) 858, 891
XspI CTAG 1 cut(s) 1430
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.