Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010643

Physical Location & Seq

Genomic Coordinates Reverse (-)

389673 .. 394905

Gene Structure 5233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1005600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3210 bp

ATGGAAGCCAGCGCCGGACTTGTTGCCGGGTCTCACAACCGCAATGAACTCGTCGTCATTCGTGGCCATGAAGAGCACAAGCCATTAAGGAACTTGGACGGACAGGTCTGCGAGATATGCGGCGACGACGTGGGGTTGACGGTGGACGGAGACTTGTTCGTGGCGTGCAACGAGTGTGGCTTCCCAGTGTGCAGGCCATGCTACGAGTACGAGCGCAGGGAAGGCTCCCAGAACTGCCCTCAGTGCAAAACCCGATACAAGCGTCTCAAAGGCAGTCCCCGTGTCGAAGGAGACGATGACGAAGAGGACCTTGATGACATTGAACACGAATTCAACATGAATCACGATCATCCGGAAGATGACCATGATCAAACTATCAGCAACGCTGCTGCCAGATCGCCGTATTACAAGCCTAATAATGAGCAGATGGCTGAAGCCATGCTCCATGGAAAGATGAGCTACGGAAGAGGCCCCGATGATCCTGATCAGGAGTCACATTTCCCACCACCTGTAATCGCCGGAGGCAGATCCCGCCCTGTAAGCGGTGAGCTCACATACTCATCGCACGCTCACGGAGATCCACAGATGTTATCATCTTCGTTGCATAAACGAGTGCATCCATATCCGGCTTCTGAGACTGGAAGTGAAAGGTGGGACGCTGAGAAGAGAGAGGGAGGTGGATGGAAAGAAAGAATGGACGACTGGAAAATGCAGCAAGGCAACCTCGGTGGCAATGATCAACCCGATGATTTGAATGATGCCGACATGGCCATGATAGATGAAGCTAGGCAGCCACTCTCAAGGAAAGTACCAATTGCTTCAAGCAAGATCAACCCTTATCGGATGATCATTGTGGCTCGGCTTTTCATTTTGGCCTTCTTCCTTCGATACAGGCTTTTGAATCCGGTCACTGACGCATTCGGTCTCTGGTTAGTGTCTGTCACATGTGAAATATGGTTTGCAGTTTCATGGATCCTTGATCAGTTCCCAAAGTGGTACCCCATTGATCGCGAGACCTACCTCGATCGCCTTTCCCTCAGGTATGAGAGGGAAGGTGAACCGAACATGCTTTGTCCGGTAGATGTGTTTGTGAGTACTGTGGATCCCATGAAGGAACCTCCACTTAATACAGCCAACACAGTTCTTTCAATTTTGTCCATGGATTACCCTGTCGATAAGATCTCATGCTACATCTCTGATGATGGAGCTTCCATGCTGACCTTCGAAGCCTTGTCAGAAACTGCAGAGTTTGCGCGCAAATGGGTACCTTTCTGCAAGAAGTTTGCGATTGAGCCTCGAGCGCCCGAGTTCTACTTCTGCGAGAAGATTGATTATCTCAAGGACAAAGTGCAGCCAACATTTGTCAAGGAACGCCGAGCTATGAAGAGAGAGTATGAGGAATTCAAGGTTAGGATAAATGCGCTTGTTGCAAAAGCCACAAAGGTTCCGCCAGAAGGGTGGATTATGCAAGATGGGACACCGTGGCCCGGAAACAACACCAAGGATCACCCCGGCATGATTCAAGTCTTTCTTGGTCACAGCGGAGGTCTTGATACTGAAGGAAATGAGCTTCCCCGTCTTGTCTACGTGTCTCGTGAGAAGAGACCGGGATTTCAACATCACAAGAAGGCCGGTGCCATGAATGCCCTGGTTCGTGTCTCCGGAGTACTTACAAATGCTCCTTTCATGCTCAACTTGGATTGTGACCATTACGTAAACAACAGCAAGGCTGCAAGAGAGGCAATGTGTTTCTTGATGGACCCCCAAATCGGAAGGAAGGTTTGCTATGTCCAGTTCCCTCAAAGATTCGATGGCATTGACAGAAATGATCGTTATGCGAACAGAAATACAGTCTTCTTCGATATTAACATGAAAGGTCTGGATGGAATTCAAGGTCCTGTGTATGTGGGAACAGGATGTGTGTTCAGAAGGCAAGCTTTATATGGCTACGATCCTCCGAAGGCTCCTAAGCGCCCGAAGATGATAAGCTGTGACTGCTGCCCGTGCTTTGGACGGCGCAAGAAGCTTCCAAAGTACTCCAAGCATGATGCAAATGGAGATGGTACAAACCTACAAGGAAGGGATGATGACAAAGAGCTACTGATGTCCCATATGAATTTTGAAAAGAAATTCGGACAGTCTGCAATTTTCGTGACTTCAACTTTGATGGAACAAGGCGGTGTCCCTCCTTCCTCAAGCCCAGCAGCGATGCTTAAAGAAGCCATTCATGTGATCAGTTGCGGTTATGAAGACAAAACCGAGTGGGGTTTAGAGCTAGGTTGGATTTACGGATCTATCACAGAGGATATCCTAACGGGATTCAAGATGCATTGCCGTGGTTGGAGATCAATTTACTGCATGCCGGATAGACCTGCATTCAAGGGTACAGCTCCCATCAACTTGTCAGATCGGCTCAACCAGGTTCTTCGTTGGGCACTTGGTTCGATTGAGATCTTTTTCAGTCGCCATAGTCCACTCTGGTATGGCTACAAGGGAGGGAAACTCAAGTGGCTAGAGCGTTTTGCTTATGTCAACACTACTGTCTACCCTTTCACCTCCCTCCCTCTTCTTGCCTATTGTATCCTCCCTGCCGTCTGCTTGCTCACCGATAAATTCATCATGCCACAGATAAGCACTTTTGCAAGTCTCTTCTTCATTGCGCTGTTTCTCTCAATCTTTGGAACCGGCATTCTTGAGCTGAGGTGGAGCGGAGTGAGCATTGAAGAATGGTGGAGAAACGAGCAGTTCTGGGTGATTGGTGGTGTCTCAGCTCACCTCTTCGCCGTTGTGCAAGGTCTTCTCAAGGTTTTAGCCGGCATTGACACCAACTTCACAGTCACAGCCAAGTCATCAGACGATGAGGATTTCGGGGAATTATACGCGTTTAAATGGACAACCCTCCTTATCCCTCCAACCACAATCTTAGTCATCAACCTTGTTGGAGTCGTTGCTGGGGTTTCGGACGCCATAAACAACGGGTACCAATCATGGGGGCCTCTGTTTGGGAAGCTCTTCTTTGCATTTTGGGTGATTGTCCATCTCTATCCCTTCCTTAAAGGTCTCATGGGGAGGCAGAACCGGACGCCAACCATTGTTGTCATTTGGTCAATTCTCCTGGCTTCCATCTTCTCTTTACTCTGGGTCCGAATCGATCCATTTGTGATGAAAACCAAGGGACCCAACACGAAGCAATGCGGAATCAACTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009507 GO:0009536 GO:0009664 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0010330 GO:0010393 GO:0010395 GO:0010400 GO:0012505 GO:0016020 GO:0016043 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0017144 GO:0022402 GO:0030243 GO:0030244 GO:0032991 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0046527 GO:0051273 GO:0051274 GO:0051301 GO:0052546 GO:0061640 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1070

Amino Acids

121.08

Weight (kDa)

6.19

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	27 - 103	1.5e-42	Zinc-binding RING-finger
Cellulose_synt	PF03552	360 - 1063	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	746 - 865	1.6e-06	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17420
fragaria_vesca	FvH4_1g00670
malus_domestica	MD02G1005600.v1.1 MD15G1150200.v1.1 MD15G1150300.v1.1
prunus_persica	Prupe.7G265400_v2.0.a1
pyrus_communis	pycom02g00450 pycom15g13420 pycom15g13430 pycom15g13440
rosa_chinensis	RchiOBHm_Chr2g0085301 RchiOBHm_Chr2g0175181
rosa_laevigata	RLG00000015676
rosa_multiflora	Rmu_sc0008510.1_g000012
rosa_rugosa	Rorug01G0459400
rosa_samantha	Rh2AG007700 Rh2BG008400 Rh2CG009100 Rh2DG009600
rosa_wichuraiana	Rw2G000740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	104
Acc36I	ACCTGC	1 cut(s)	2380
Acc65I	GGTACC	3 cut(s)	996, 1264, 2979
AccB1I	GGYRCC	4 cut(s)	996, 1264, 1634, 2979
AccBSI	CCGCTC	1 cut(s)	2709
AccI	GTMKAC	2 cut(s)	1584, 2544
AccII	CGCG	3 cut(s)	1011, 1255, 2882
AccIII	TCCGGA	2 cut(s)	352, 1661
AcoI	YGGCCR	2 cut(s)	64, 768
AcsI	RAATTY	6 cut(s)	329, 1400, 1887, 2116, 2129, 2612
AcuI	CTGAAG	2 cut(s)	453, 1578
AcyI	GRCGYC	2 cut(s)	2964, 3083
AfiI	CCNNNNNNNGG	7 cut(s)	542, 1454, 1487, 1769, 2009, 2989, 3002
AflIII	ACRYGT	3 cut(s)	944, 1587, 2880
AjiI	CACGTC	1 cut(s)	130
AjnI	CCWGG	3 cut(s)	1647, 2418, 3114
Alw21I	GWGCWC	2 cut(s)	78, 552
AlwNI	CAGNNNCTG	1 cut(s)	1241
Ama87I	CYCGRG	2 cut(s)	1296, 1304
Aor13HI	TCCGGA	2 cut(s)	352, 1661
AoxI	GGCC	8 cut(s)	64, 194, 469, 768, 873, 1484, 1629, 2993
ApeKI	GCWGC	8 cut(s)	386, 389, 712, 790, 1351, 1730, 1998, 2204
ApoI	RAATTY	6 cut(s)	329, 1400, 1887, 2116, 2129, 2612
ArsI	GACNNNNNNTTYG	2 cut(s)	2146, 2178
Asp700I	GAANNNNTTC	2 cut(s)	2223, 3011
Asp718I	GGTACC	3 cut(s)	996, 1264, 2979
AspLEI	GCGC	9 cut(s)	14, 216, 1255, 1257, 1303, 1423, 1974, 2019, 2662
AspS9I	GGNCC	8 cut(s)	307, 470, 1485, 1759, 1895, 2993, 3142, 3176
AsuC2I	CCSGG	4 cut(s)	28, 1488, 1512, 1608
AsuHPI	GGTGA	8 cut(s)	557, 1067, 1499, 2545, 2596, 2764, 2765, 3040
AsuII	TTCGAA	1 cut(s)	1224
AvaI	CYCGRG	2 cut(s)	1296, 1304
AvaII	GGWCC	5 cut(s)	307, 1759, 1895, 3142, 3176
AxyI	CCTNAGG	1 cut(s)	1037
BaeGI	GKGCMC	1 cut(s)	2437
BaeI	ACNNNNGTAYC	2 cut(s)	247, 280
BalI	TGGCCA	2 cut(s)	66, 770
BamHI	GGATCC	2 cut(s)	972, 1102
BanI	GGYRCC	4 cut(s)	996, 1264, 1634, 2979
BanII	GRGCYC	1 cut(s)	552
BauI	CACGAG	1 cut(s)	1593
BbsI	GAAGAC	3 cut(s)	1846, 2256, 2789
Bbv12I	GWGCWC	2 cut(s)	78, 552
BbvCI	CCTCAGC	1 cut(s)	2699
BbvI	GCAGC	8 cut(s)	373, 376, 724, 802, 1363, 1717, 1985, 2216
BceAI	ACGGC	5 cut(s)	385, 2030, 2319, 2576, 2768
BcgI	CGANNNNNNTGC	4 cut(s)	31, 65, 2424, 2458
BciT130I	CCWGG	3 cut(s)	1649, 2420, 3116
BciVI	GTATCC	1 cut(s)	2591
BclI	TGATCA	6 cut(s)	367, 484, 736, 846, 979, 2232
BcnI	CCSGG	4 cut(s)	28, 1488, 1512, 1608
BfaI	CTAG	3 cut(s)	786, 2276, 2513
BfmI	CTRYAG	1 cut(s)	1242
BfoI	RGCGCY	3 cut(s)	15, 1304, 1975
BfuAI	ACCTGC	1 cut(s)	2380
BfuI	GTATCC	1 cut(s)	2591
BglI	GCCNNNNNGGC	1 cut(s)	767
BglII	AGATCT	2 cut(s)	1179, 2451
BisI	GCNGC	9 cut(s)	121, 387, 390, 713, 791, 1352, 1731, 1999, 2205
BlsI	GCNGC	9 cut(s)	122, 388, 391, 714, 792, 1353, 1732, 2000, 2206
BmcAI	AGTACT	3 cut(s)	1096, 1668, 2036
Bme1390I	CCNGG	7 cut(s)	28, 1488, 1512, 1608, 1649, 2420, 3116
Bme18I	GGWCC	5 cut(s)	307, 1759, 1895, 3142, 3176
BmeT110I	CYCGRG	2 cut(s)	1296, 1304
BmgBI	CACGTC	1 cut(s)	130
BmgT120I	GGNCC	8 cut(s)	307, 470, 1485, 1759, 1895, 2993, 3142, 3176
BmrFI	CCNGG	7 cut(s)	28, 1488, 1512, 1608, 1649, 2420, 3116
BmrI	ACTGGG	1 cut(s)	179
BmsI	GCATC	5 cut(s)	625, 748, 2038, 2199, 2316
BmuI	ACTGGG	1 cut(s)	179
BpiI	GAAGAC	3 cut(s)	1846, 2256, 2789
BplI	GAGNNNNNCTC	2 cut(s)	2487, 2519
Bpu10I	CCTNAGC	2 cut(s)	1968, 2699
Bpu14I	TTCGAA	1 cut(s)	1224
BpuEI	CTTGAG	6 cut(s)	784, 1322, 2179, 2489, 2714, 2786
BpuMI	CCSGG	4 cut(s)	28, 1488, 1512, 1608
Bsa29I	ATCGAT	1 cut(s)	3150
BsaAI	YACGTR	2 cut(s)	1588, 1714
BsaBI	GATNNNNATC	2 cut(s)	483, 2450
BsaHI	GRCGYC	2 cut(s)	2964, 3083
BsaI	GGTCTC	5 cut(s)	36, 929, 1007, 1597, 3065
BsaJI	CCNNGG	9 cut(s)	445, 724, 1158, 1481, 1500, 1510, 1647, 2335, 3171
BsaWI	WCCGGW	5 cut(s)	352, 904, 1075, 1661, 3078
BsaXI	ACNNNNNCTCC	2 cut(s)	1663, 1693
Bsc4I	CCNNNNNNNGG	7 cut(s)	542, 1454, 1487, 1769, 2009, 2989, 3002
Bse118I	RCCGGY	3 cut(s)	1631, 2684, 2813
Bse1I	ACTGG	4 cut(s)	185, 643, 707, 1792
Bse21I	CCTNAGG	1 cut(s)	1037
Bse3DI	GCAATG	6 cut(s)	49, 739, 1749, 2329, 2655, 3197
Bse8I	GATNNNNATC	2 cut(s)	483, 2450
BseAI	TCCGGA	2 cut(s)	352, 1661
BseBI	CCWGG	3 cut(s)	1649, 2420, 3116
BseCI	ATCGAT	1 cut(s)	3150
BseDI	CCNNGG	9 cut(s)	445, 724, 1158, 1481, 1500, 1510, 1647, 2335, 3171
BseGI	GGATG	7 cut(s)	349, 616, 686, 849, 1888, 1922, 2089
BseJI	GATNNNNATC	2 cut(s)	483, 2450
BseLI	CCNNNNNNNGG	7 cut(s)	542, 1454, 1487, 1769, 2009, 2989, 3002
BseMI	GCAATG	6 cut(s)	49, 739, 1749, 2329, 2655, 3197
BseMII	CTCAG	6 cut(s)	254, 624, 651, 1051, 2690, 2781
BseNI	ACTGG	4 cut(s)	185, 643, 707, 1792
BsePI	GCGCGC	1 cut(s)	1253
BseSI	GKGCMC	1 cut(s)	2437
BseXI	GCAGC	8 cut(s)	373, 376, 724, 802, 1363, 1717, 1985, 2216
BseYI	CCCAGC	2 cut(s)	2200, 2951
BsgI	GTGCAG	2 cut(s)	211, 1370
Bsh1236I	CGCG	3 cut(s)	1011, 1255, 2882
Bsh1285I	CGRYCG	1 cut(s)	1027
BshFI	GGCC	8 cut(s)	66, 196, 471, 770, 875, 1486, 1631, 2995
BshNI	GGYRCC	4 cut(s)	996, 1264, 1634, 2979
BshVI	ATCGAT	1 cut(s)	3150
BsiEI	CGRYCG	1 cut(s)	1027
BsiHKAI	GWGCWC	2 cut(s)	78, 552
BsiHKCI	CYCGRG	2 cut(s)	1296, 1304
BslFI	GGGAC	6 cut(s)	261, 668, 1489, 2092, 2168, 3189
BslI	CCNNNNNNNGG	7 cut(s)	542, 1454, 1487, 1769, 2009, 2989, 3002
BsmBI	CGTCTC	2 cut(s)	269, 285
BsmFI	GGGAC	6 cut(s)	261, 668, 1489, 2092, 2168, 3189
BsmI	GAATGC	4 cut(s)	917, 1648, 2375, 2688
BsnI	GGCC	8 cut(s)	66, 196, 471, 770, 875, 1486, 1631, 2995
Bso31I	GGTCTC	5 cut(s)	36, 929, 1007, 1597, 3065
BsoBI	CYCGRG	2 cut(s)	1296, 1304
Bsp119I	TTCGAA	1 cut(s)	1224
Bsp1286I	GDGCHC	3 cut(s)	78, 552, 2437
Bsp13I	TCCGGA	2 cut(s)	352, 1661
Bsp19I	CCATGG	2 cut(s)	445, 1158
Bsp68I	TCGCGA	1 cut(s)	1011
BspANI	GGCC	8 cut(s)	66, 196, 471, 770, 875, 1486, 1631, 2995
BspCNI	CTCAG	6 cut(s)	253, 625, 652, 1050, 2691, 2780
BspDI	ATCGAT	1 cut(s)	3150
BspEI	TCCGGA	2 cut(s)	352, 1661
BspFNI	CGCG	3 cut(s)	1011, 1255, 2882
BspMAI	CTGCAG	1 cut(s)	1246
BspMI	ACCTGC	1 cut(s)	2380
BspQI	GCTCTTC	2 cut(s)	66, 3017
BspT104I	TTCGAA	1 cut(s)	1224
BspT107I	GGYRCC	4 cut(s)	996, 1264, 1634, 2979
BspTNI	GGTCTC	5 cut(s)	36, 929, 1007, 1597, 3065
BsrBI	CCGCTC	1 cut(s)	2709
BsrDI	GCAATG	6 cut(s)	49, 739, 1749, 2329, 2655, 3197
BsrFI	RCCGGY	3 cut(s)	1631, 2684, 2813
BsrI	ACTGG	4 cut(s)	185, 643, 707, 1792
BssAI	RCCGGY	3 cut(s)	1631, 2684, 2813
BssECI	CCNNGG	9 cut(s)	445, 724, 1158, 1481, 1500, 1510, 1647, 2335, 3171
BssHII	GCGCGC	1 cut(s)	1253
BssNI	GRCGYC	2 cut(s)	2964, 3083
BssSI	CACGAG	1 cut(s)	1593
BssT1I	CCWWGG	4 cut(s)	445, 1158, 1500, 3171
Bst2BI	CACGAG	1 cut(s)	1593
Bst2UI	CCWGG	3 cut(s)	1649, 2420, 3116
Bst4CI	ACNGT	8 cut(s)	142, 1099, 1141, 1482, 1852, 2139, 2542, 2836
BstACI	GRCGYC	2 cut(s)	2964, 3083
BstAPI	GCANNNNNTGC	2 cut(s)	198, 1250
BstBAI	YACGTR	2 cut(s)	1588, 1714
BstBI	TTCGAA	1 cut(s)	1224
BstC8I	GCNNGC	9 cut(s)	10, 166, 194, 567, 1255, 1935, 2360, 2600, 2815
BstDEI	CTNAG	8 cut(s)	240, 633, 660, 1037, 1968, 2699, 2767, 2923
BstDSI	CCRYGG	4 cut(s)	445, 1158, 1481, 2335
BstF5I	GGATG	7 cut(s)	349, 616, 686, 849, 1888, 1922, 2089
BstFNI	CGCG	3 cut(s)	1011, 1255, 2882
BstH2I	RGCGCY	3 cut(s)	15, 1304, 1975
BstHHI	GCGC	9 cut(s)	14, 216, 1255, 1257, 1303, 1423, 1974, 2019, 2662
BstMCI	CGRYCG	1 cut(s)	1027
BstNI	CCWGG	3 cut(s)	1649, 2420, 3116
BstNSI	RCATGY	3 cut(s)	948, 1069, 2362
BstSCI	CCNGG	7 cut(s)	26, 1486, 1510, 1606, 1647, 2418, 3114
BstSFI	CTRYAG	1 cut(s)	1242
BstSLI	GKGCMC	1 cut(s)	2437
BstSNI	TACGTA	1 cut(s)	1714
BstUI	CGCG	3 cut(s)	1011, 1255, 2882
BstV1I	GCAGC	8 cut(s)	373, 376, 724, 802, 1363, 1717, 1985, 2216
BstV2I	GAAGAC	3 cut(s)	1846, 2256, 2789
BstX2I	RGATCY	7 cut(s)	527, 577, 972, 1102, 1179, 2291, 2451
BstXI	CCANNNNNNTGG	1 cut(s)	1458
BstYI	RGATCY	7 cut(s)	527, 577, 972, 1102, 1179, 2291, 2451
Bsu15I	ATCGAT	1 cut(s)	3150
Bsu36I	CCTNAGG	1 cut(s)	1037
BsuI	GTATCC	1 cut(s)	2591
BsuRI	GGCC	8 cut(s)	66, 196, 471, 770, 875, 1486, 1631, 2995
BsuTUI	ATCGAT	1 cut(s)	3150
BtgI	CCRYGG	4 cut(s)	445, 1158, 1481, 2335
BtgZI	GCGATG	2 cut(s)	546, 2222
BtrI	CACGTC	1 cut(s)	130
BtsCI	GGATG	7 cut(s)	349, 616, 686, 849, 1888, 1922, 2089
BtsIMutI	CAGTG	3 cut(s)	192, 248, 909
BtuMI	TCGCGA	1 cut(s)	1011
BveI	ACCTGC	1 cut(s)	2380
Cac8I	GCNNGC	9 cut(s)	10, 166, 194, 567, 1255, 1935, 2360, 2600, 2815
CaiI	CAGNNNCTG	1 cut(s)	1241
CfoI	GCGC	9 cut(s)	14, 216, 1255, 1257, 1303, 1423, 1974, 2019, 2662
Cfr10I	RCCGGY	3 cut(s)	1631, 2684, 2813
Cfr13I	GGNCC	8 cut(s)	307, 470, 1485, 1759, 1895, 2993, 3142, 3176
ClaI	ATCGAT	1 cut(s)	3150
CseI	GACGC	5 cut(s)	251, 665, 923, 2972, 3091
CsiI	ACCWGGT	1 cut(s)	2418
CspCI	CAANNNNNGTGG	4 cut(s)	157, 192, 709, 744
DdeI	CTNAG	8 cut(s)	240, 633, 660, 1037, 1968, 2699, 2767, 2923
DraI	TTTAAA	1 cut(s)	2887
DrdI	GACNNNNNNGTC	1 cut(s)	104
DseDI	GACNNNNNNGTC	1 cut(s)	104
EaeI	YGGCCR	2 cut(s)	64, 768
EciI	GGCGGA	1 cut(s)	1437
Ecl136II	GAGCTC	1 cut(s)	550
Eco105I	TACGTA	1 cut(s)	1714
Eco130I	CCWWGG	4 cut(s)	445, 1158, 1500, 3171
Eco24I	GRGCYC	1 cut(s)	552
Eco31I	GGTCTC	5 cut(s)	36, 929, 1007, 1597, 3065
Eco32I	GATATC	1 cut(s)	2308
Eco47I	GGWCC	5 cut(s)	307, 1759, 1895, 3142, 3176
Eco53kI	GAGCTC	1 cut(s)	550
Eco57I	CTGAAG	2 cut(s)	453, 1578
Eco81I	CCTNAGG	1 cut(s)	1037
Eco88I	CYCGRG	2 cut(s)	1296, 1304
EcoICRI	GAGCTC	1 cut(s)	550
EcoO109I	RGGNCCY	5 cut(s)	307, 470, 1895, 2993, 3176
EcoRI	GAATTC	3 cut(s)	329, 1400, 1887
EcoRII	CCWGG	3 cut(s)	1647, 2418, 3114
EcoRV	GATATC	1 cut(s)	2308
EcoT14I	CCWWGG	4 cut(s)	445, 1158, 1500, 3171
EcoT22I	ATGCAT	1 cut(s)	2331
EcoT38I	GRGCYC	1 cut(s)	552
ErhI	CCWWGG	4 cut(s)	445, 1158, 1500, 3171
Esp3I	CGTCTC	2 cut(s)	269, 285
FalI	AAGNNNNNCTT	8 cut(s)	294, 326, 1515, 1547, 1921, 1953, 2999, 3031
FaqI	GGGAC	6 cut(s)	261, 668, 1489, 2092, 2168, 3189
FauI	CCCGC	1 cut(s)	539
FauNDI	CATATG	1 cut(s)	2112
FbaI	TGATCA	6 cut(s)	367, 484, 736, 846, 979, 2232
FblI	GTMKAC	2 cut(s)	1584, 2544
Fnu4HI	GCNGC	9 cut(s)	121, 387, 390, 713, 791, 1352, 1731, 1999, 2205
FokI	GGATG	7 cut(s)	336, 603, 693, 856, 1895, 1929, 2096
FriOI	GRGCYC	1 cut(s)	552
Fsp4HI	GCNGC	9 cut(s)	121, 387, 390, 713, 791, 1352, 1731, 1999, 2205
FspBI	CTAG	3 cut(s)	786, 2276, 2513
GlaI	GCGC	9 cut(s)	13, 215, 1254, 1256, 1302, 1422, 1973, 2018, 2661
GluI	GCNGC	9 cut(s)	121, 387, 390, 713, 791, 1352, 1731, 1999, 2205
GsaI	CCCAGC	2 cut(s)	2204, 2955
HaeII	RGCGCY	3 cut(s)	15, 1304, 1975
HaeIII	GGCC	8 cut(s)	66, 196, 471, 770, 875, 1486, 1631, 2995
HgaI	GACGC	5 cut(s)	251, 665, 923, 2972, 3091
HhaI	GCGC	9 cut(s)	14, 216, 1255, 1257, 1303, 1423, 1974, 2019, 2662
Hin1I	GRCGYC	2 cut(s)	2964, 3083
Hin6I	GCGC	9 cut(s)	12, 214, 1253, 1255, 1301, 1421, 1972, 2017, 2660
HinP1I	GCGC	9 cut(s)	12, 214, 1253, 1255, 1301, 1421, 1972, 2017, 2660
HincII	GTYRAC	2 cut(s)	138, 2533
HindII	GTYRAC	2 cut(s)	138, 2533
HindIII	AAGCTT	2 cut(s)	1935, 2024
HinfI	GANTC	9 cut(s)	340, 491, 901, 1519, 1806, 2319, 2943, 3147, 3198
HphI	GGTGA	8 cut(s)	557, 1067, 1499, 2545, 2596, 2764, 2765, 3040
Hpy166II	GTNNAC	8 cut(s)	138, 145, 1058, 1585, 1717, 2474, 2533, 2545
Hpy8I	GTNNAC	8 cut(s)	138, 145, 1058, 1585, 1717, 2474, 2533, 2545
Hpy99I	CGWCG	3 cut(s)	56, 128, 131
HpyCH4III	ACNGT	8 cut(s)	142, 1099, 1141, 1482, 1852, 2139, 2542, 2836
HpyCH4IV	ACGT	3 cut(s)	129, 1587, 1713
HpyF3I	CTNAG	8 cut(s)	240, 633, 660, 1037, 1968, 2699, 2767, 2923
HpySE526I	ACGT	3 cut(s)	129, 1587, 1713
Hsp92I	GRCGYC	2 cut(s)	2964, 3083
HspAI	GCGC	9 cut(s)	12, 214, 1253, 1255, 1301, 1421, 1972, 2017, 2660
KflI	GGGWCCC	1 cut(s)	3176
Kpn2I	TCCGGA	2 cut(s)	352, 1661
KpnI	GGTACC	3 cut(s)	1000, 1268, 2983
KroI	GCCGGC	1 cut(s)	2813
KroNI	GCCGGC	1 cut(s)	2815
Ksp22I	TGATCA	6 cut(s)	367, 484, 736, 846, 979, 2232
LguI	GCTCTTC	2 cut(s)	66, 3017
LmnI	GCTCC	7 cut(s)	230, 447, 1205, 1684, 1969, 2395, 2706
Lsp1109I	GCAGC	8 cut(s)	373, 376, 724, 802, 1363, 1717, 1985, 2216
LweI	GCATC	5 cut(s)	625, 748, 2038, 2199, 2316
MabI	ACCWGGT	1 cut(s)	2418
MaeI	CTAG	3 cut(s)	786, 2276, 2513
MaeII	ACGT	3 cut(s)	129, 1587, 1713
MaeIII	GTNAC	8 cut(s)	492, 907, 940, 1535, 1703, 1991, 2152, 2836
MbiI	CCGCTC	1 cut(s)	2709
MfeI	CAATTG	1 cut(s)	813
MflI	RGATCY	7 cut(s)	527, 577, 972, 1102, 1179, 2291, 2451
MhlI	GDGCHC	3 cut(s)	78, 552, 2437
MlsI	TGGCCA	2 cut(s)	66, 770
MluI	ACGCGT	1 cut(s)	2880
MluNI	TGGCCA	2 cut(s)	66, 770
MlyI	GAGTC	2 cut(s)	500, 2952
MmeI	TCCRAC	4 cut(s)	2261, 2321, 2920, 2936
Mox20I	TGGCCA	2 cut(s)	66, 770
Mph1103I	ATGCAT	1 cut(s)	2331
MroI	TCCGGA	2 cut(s)	352, 1661
MroNI	GCCGGC	1 cut(s)	2813
MroXI	GAANNNNTTC	2 cut(s)	2223, 3011
MscI	TGGCCA	2 cut(s)	66, 770
MseI	TTAA	6 cut(s)	86, 1125, 1866, 2214, 2886, 3054
MslI	CAYNNNNRTG	3 cut(s)	770, 2228, 2334
Msp20I	TGGCCA	2 cut(s)	66, 770
MspA1I	CMGCKG	1 cut(s)	1542
MspR9I	CCNGG	7 cut(s)	28, 1488, 1512, 1608, 1649, 2420, 3116
MunI	CAATTG	1 cut(s)	813
Mva1269I	GAATGC	4 cut(s)	917, 1648, 2375, 2688
MvaI	CCWGG	3 cut(s)	1649, 2420, 3116
MvnI	CGCG	3 cut(s)	1011, 1255, 2882
NaeI	GCCGGC	1 cut(s)	2815
NciI	CCSGG	4 cut(s)	28, 1488, 1512, 1608
NcoI	CCATGG	2 cut(s)	445, 1158
NdeI	CATATG	1 cut(s)	2112
NgoMIV	GCCGGC	1 cut(s)	2813
NmeAIII	GCCGAG	2 cut(s)	838, 1400
NmuCI	GTSAC	8 cut(s)	492, 907, 940, 1535, 1703, 1991, 2152, 2836
NruI	TCGCGA	1 cut(s)	1011
NsiI	ATGCAT	1 cut(s)	2331
NspI	RCATGY	3 cut(s)	948, 1069, 2362
NspV	TTCGAA	1 cut(s)	1224
PaeI	GCATGC	1 cut(s)	2362
PaeR7I	CTCGAG	1 cut(s)	1296
PauI	GCGCGC	1 cut(s)	1253
PciI	ACATGT	1 cut(s)	944
PciSI	GCTCTTC	2 cut(s)	66, 3017
PcsI	WCGNNNNNNNCGW	1 cut(s)	291
PctI	GAATGC	4 cut(s)	917, 1648, 2375, 2688
PdiI	GCCGGC	1 cut(s)	2815
PdmI	GAANNNNTTC	2 cut(s)	2223, 3011
PfeI	GAWTC	7 cut(s)	340, 901, 1519, 1806, 2319, 3147, 3198
PkrI	GCNGC	9 cut(s)	122, 388, 391, 714, 792, 1353, 1732, 2000, 2206
Ple19I	CGATCG	1 cut(s)	1027
PleI	GAGTC	2 cut(s)	499, 2951
PpsI	GAGTC	2 cut(s)	499, 2951
Ppu21I	YACGTR	2 cut(s)	1588, 1714
PpuMI	RGGWCCY	3 cut(s)	307, 1895, 3176
PscI	ACATGT	1 cut(s)	944
Psp124BI	GAGCTC	1 cut(s)	552
Psp5II	RGGWCCY	3 cut(s)	307, 1895, 3176
Psp6I	CCWGG	3 cut(s)	1647, 2418, 3114
PspFI	CCCAGC	2 cut(s)	2200, 2951
PspGI	CCWGG	3 cut(s)	1647, 2418, 3114
PspPI	GGNCC	8 cut(s)	307, 470, 1485, 1759, 1895, 2993, 3142, 3176
PspPPI	RGGWCCY	3 cut(s)	307, 1895, 3176
PspXI	VCTCGAGB	1 cut(s)	1296
PstI	CTGCAG	1 cut(s)	1246
PstNI	CAGNNNCTG	1 cut(s)	1241
PsuI	RGATCY	7 cut(s)	527, 577, 972, 1102, 1179, 2291, 2451
PteI	GCGCGC	1 cut(s)	1253
PvuI	CGATCG	1 cut(s)	1027
RruI	TCGCGA	1 cut(s)	1011
RseI	CAYNNNNRTG	3 cut(s)	770, 2228, 2334
SacI	GAGCTC	1 cut(s)	552
SapI	GCTCTTC	2 cut(s)	66, 3017
SaqAI	TTAA	6 cut(s)	86, 1125, 1866, 2214, 2886, 3054
SatI	GCNGC	9 cut(s)	121, 387, 390, 713, 791, 1352, 1731, 1999, 2205
Sau96I	GGNCC	8 cut(s)	307, 470, 1485, 1759, 1895, 2993, 3142, 3176
ScaI	AGTACT	3 cut(s)	1096, 1668, 2036
SchI	GAGTC	2 cut(s)	500, 2952
ScrFI	CCNGG	7 cut(s)	28, 1488, 1512, 1608, 1649, 2420, 3116
SduI	GDGCHC	3 cut(s)	78, 552, 2437
SexAI	ACCWGGT	1 cut(s)	2418
SfaNI	GCATC	5 cut(s)	625, 748, 2038, 2199, 2316
SfcI	CTRYAG	1 cut(s)	1242
Sfr274I	CTCGAG	1 cut(s)	1296
SfuI	TTCGAA	1 cut(s)	1224
SinI	GGWCC	5 cut(s)	307, 1759, 1895, 3142, 3176
SlaI	CTCGAG	1 cut(s)	1296
SmiMI	CAYNNNNRTG	3 cut(s)	770, 2228, 2334
SmlI	CTYRAG	7 cut(s)	799, 1296, 1337, 2194, 2504, 2693, 2801
SmoI	CTYRAG	7 cut(s)	799, 1296, 1337, 2194, 2504, 2693, 2801
SnaBI	TACGTA	1 cut(s)	1714
SphI	GCATGC	1 cut(s)	2362
SspMI	CTAG	3 cut(s)	786, 2276, 2513
SstI	GAGCTC	1 cut(s)	552
StyD4I	CCNGG	7 cut(s)	26, 1486, 1510, 1606, 1647, 2418, 3114
StyI	CCWWGG	4 cut(s)	445, 1158, 1500, 3171
TaaI	ACNGT	8 cut(s)	142, 1099, 1141, 1482, 1852, 2139, 2542, 2836
TaiI	ACGT	3 cut(s)	132, 1590, 1716
TaqII	GACCGA	1 cut(s)	911
TatI	WGTACW	3 cut(s)	1094, 1666, 2034
TauI	GCSGC	1 cut(s)	123
TfiI	GAWTC	7 cut(s)	340, 901, 1519, 1806, 2319, 3147, 3198
Tru1I	TTAA	6 cut(s)	86, 1125, 1866, 2214, 2886, 3054
Tru9I	TTAA	6 cut(s)	86, 1125, 1866, 2214, 2886, 3054
TscAI	CASTG	3 cut(s)	192, 248, 916
TseFI	GTSAC	8 cut(s)	492, 907, 940, 1535, 1703, 1991, 2152, 2836
TseI	GCWGC	8 cut(s)	386, 389, 712, 790, 1351, 1730, 1998, 2204
Tsp45I	GTSAC	8 cut(s)	492, 907, 940, 1535, 1703, 1991, 2152, 2836
TspGWI	ACGGA	5 cut(s)	114, 162, 477, 588, 2304
TspRI	CASTG	3 cut(s)	192, 248, 916
VpaK11BI	GGWCC	5 cut(s)	307, 1759, 1895, 3142, 3176
XapI	RAATTY	6 cut(s)	329, 1400, 1887, 2116, 2129, 2612
XceI	RCATGY	3 cut(s)	948, 1069, 2362
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1645
XhoI	CTCGAG	1 cut(s)	1296
XmiI	GTMKAC	2 cut(s)	1584, 2544
XmnI	GAANNNNTTC	2 cut(s)	2223, 3011
XspI	CTAG	3 cut(s)	786, 2276, 2513
ZrmI	AGTACT	3 cut(s)	1096, 1668, 2036
Zsp2I	ATGCAT	1 cut(s)	2331

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.